LOCUS       DAYYOA010000001        81742 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000001 DAYYOA010000000
VERSION     DAYYOA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.001"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..526
                     /locus_tag="ACHBKA_000001"
     CDS             8..526
                     /locus_tag="ACHBKA_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9443734.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            654..1337
                     /locus_tag="ACHBKA_000002"
     CDS             654..1337
                     /locus_tag="ACHBKA_000002"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM9443735.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1646..1719
                     /locus_tag="ACHBKA_000003"
     tRNA            1646..1719
                     /locus_tag="ACHBKA_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1680..1682,aa:Arg,seq:tct)
     gene            1878..2237
                     /locus_tag="ACHBKA_000004"
     CDS             1878..2237
                     /locus_tag="ACHBKA_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM9443736.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2360..3991
                     /locus_tag="ACHBKA_000005"
     CDS             2360..3991
                     /locus_tag="ACHBKA_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM9443737.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4053..4694)
                     /locus_tag="ACHBKA_000006"
     CDS             complement(4053..4694)
                     /locus_tag="ACHBKA_000006"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9443738.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4880..4956
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4976..5284
                     /gene="rplU"
                     /locus_tag="ACHBKA_000007"
     CDS             4976..5284
                     /gene="rplU"
                     /locus_tag="ACHBKA_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM9443739.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5311..5655
                     /locus_tag="ACHBKA_000008"
     CDS             5311..5655
                     /locus_tag="ACHBKA_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM9443740.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5668..5961
                     /gene="rpmA"
                     /locus_tag="ACHBKA_000009"
     CDS             5668..5961
                     /gene="rpmA"
                     /locus_tag="ACHBKA_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM9443741.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6395..9202
                     /locus_tag="ACHBKA_000010"
     CDS             6395..9202
                     /locus_tag="ACHBKA_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9443742.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9393..9884
                     /locus_tag="ACHBKA_000011"
     CDS             9393..9884
                     /locus_tag="ACHBKA_000011"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9443743.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9987..10976
                     /locus_tag="ACHBKA_000012"
     CDS             9987..10976
                     /locus_tag="ACHBKA_000012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9443744.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11009..11812
                     /locus_tag="ACHBKA_000013"
     CDS             11009..11812
                     /locus_tag="ACHBKA_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM9443745.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11833..12747
                     /locus_tag="ACHBKA_000014"
     CDS             11833..12747
                     /locus_tag="ACHBKA_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9443746.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12830..13204
                     /locus_tag="ACHBKA_000015"
     CDS             12830..13204
                     /locus_tag="ACHBKA_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM9443747.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13343..13588
                     /locus_tag="ACHBKA_000016"
     CDS             13343..13588
                     /locus_tag="ACHBKA_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9443748.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13760..14824
                     /locus_tag="ACHBKA_000017"
     CDS             13760..14824
                     /locus_tag="ACHBKA_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM9443749.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15070..15495
                     /locus_tag="ACHBKA_000018"
     CDS             15070..15495
                     /locus_tag="ACHBKA_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9443750.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15488..15937
                     /gene="nusB"
                     /locus_tag="ACHBKA_000019"
     CDS             15488..15937
                     /gene="nusB"
                     /locus_tag="ACHBKA_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM9443751.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16034..16888
                     /locus_tag="ACHBKA_000020"
     CDS             16034..16888
                     /locus_tag="ACHBKA_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM9443752.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16889..18226
                     /gene="xseA"
                     /locus_tag="ACHBKA_000021"
     CDS             16889..18226
                     /gene="xseA"
                     /locus_tag="ACHBKA_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM9443753.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18231..18455
                     /locus_tag="ACHBKA_000022"
     CDS             18231..18455
                     /locus_tag="ACHBKA_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM9443754.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18457..19347
                     /locus_tag="ACHBKA_000023"
     CDS             18457..19347
                     /locus_tag="ACHBKA_000023"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9443755.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19356..20174
                     /locus_tag="ACHBKA_000024"
     CDS             19356..20174
                     /locus_tag="ACHBKA_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM9443756.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20281..20730
                     /locus_tag="ACHBKA_000025"
     CDS             20281..20730
                     /locus_tag="ACHBKA_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9443757.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20748..22430
                     /gene="recN"
                     /locus_tag="ACHBKA_000026"
     CDS             20748..22430
                     /gene="recN"
                     /locus_tag="ACHBKA_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM9443758.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22485..22601)
                     /locus_tag="ACHBKA_000027"
     CDS             complement(22485..22601)
                     /locus_tag="ACHBKA_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM9443759.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22674..23630)
                     /locus_tag="ACHBKA_000028"
     CDS             complement(22674..23630)
                     /locus_tag="ACHBKA_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9443760.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23801..24169
                     /locus_tag="ACHBKA_000029"
     CDS             23801..24169
                     /locus_tag="ACHBKA_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM9443761.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24339..24770
                     /gene="mraZ"
                     /locus_tag="ACHBKA_000030"
     CDS             24339..24770
                     /gene="mraZ"
                     /locus_tag="ACHBKA_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM9443762.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24791..25750
                     /gene="rsmH"
                     /locus_tag="ACHBKA_000031"
     CDS             24791..25750
                     /gene="rsmH"
                     /locus_tag="ACHBKA_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM9443763.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25755..26156
                     /gene="ftsL"
                     /locus_tag="ACHBKA_000032"
     CDS             25755..26156
                     /gene="ftsL"
                     /locus_tag="ACHBKA_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM9443764.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26157..28349
                     /locus_tag="ACHBKA_000033"
     CDS             26157..28349
                     /locus_tag="ACHBKA_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9443765.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28375..29337
                     /gene="mraY"
                     /locus_tag="ACHBKA_000034"
     CDS             28375..29337
                     /gene="mraY"
                     /locus_tag="ACHBKA_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM9443766.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29351..30712
                     /gene="murD"
                     /locus_tag="ACHBKA_000035"
     CDS             29351..30712
                     /gene="murD"
                     /locus_tag="ACHBKA_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM9443767.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30738..31826
                     /gene="murG"
                     /locus_tag="ACHBKA_000036"
     CDS             30738..31826
                     /gene="murG"
                     /locus_tag="ACHBKA_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9443768.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31841..33061
                     /locus_tag="ACHBKA_000037"
     CDS             31841..33061
                     /locus_tag="ACHBKA_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM9443769.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33217..34542
                     /gene="ftsA"
                     /locus_tag="ACHBKA_000038"
     CDS             33217..34542
                     /gene="ftsA"
                     /locus_tag="ACHBKA_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM9443770.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34565..35806
                     /gene="ftsZ"
                     /locus_tag="ACHBKA_000039"
     CDS             34565..35806
                     /gene="ftsZ"
                     /locus_tag="ACHBKA_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM9443771.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35823..36500
                     /locus_tag="ACHBKA_000040"
     CDS             35823..36500
                     /locus_tag="ACHBKA_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM9443772.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36514..37113
                     /locus_tag="ACHBKA_000041"
     CDS             36514..37113
                     /locus_tag="ACHBKA_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM9443773.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37194..37415
                     /locus_tag="ACHBKA_000042"
     CDS             37194..37415
                     /locus_tag="ACHBKA_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM9443774.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37464..38246
                     /locus_tag="ACHBKA_000043"
     CDS             37464..38246
                     /locus_tag="ACHBKA_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM9443775.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38267..38974
                     /locus_tag="ACHBKA_000044"
     CDS             38267..38974
                     /locus_tag="ACHBKA_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM9443776.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39236..42034
                     /gene="ileS"
                     /locus_tag="ACHBKA_000045"
     CDS             39236..42034
                     /gene="ileS"
                     /locus_tag="ACHBKA_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM9443777.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42083..43609)
                     /gene="zwf"
                     /locus_tag="ACHBKA_000046"
     CDS             complement(42083..43609)
                     /gene="zwf"
                     /locus_tag="ACHBKA_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM9443778.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43624..44271)
                     /locus_tag="ACHBKA_000047"
     CDS             complement(43624..44271)
                     /locus_tag="ACHBKA_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9443779.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44455..44784)
                     /locus_tag="ACHBKA_000048"
     CDS             complement(44455..44784)
                     /locus_tag="ACHBKA_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM9443780.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44961..45689
                     /gene="sfsA"
                     /locus_tag="ACHBKA_000049"
     CDS             44961..45689
                     /gene="sfsA"
                     /locus_tag="ACHBKA_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM9443781.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45705..46718
                     /locus_tag="ACHBKA_000050"
     CDS             45705..46718
                     /locus_tag="ACHBKA_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9443782.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46718..47995
                     /locus_tag="ACHBKA_000051"
     CDS             46718..47995
                     /locus_tag="ACHBKA_000051"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9443783.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48058..50760
                     /locus_tag="ACHBKA_000052"
     CDS             48058..50760
                     /locus_tag="ACHBKA_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9443784.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50912..51229
                     /locus_tag="ACHBKA_000053"
     CDS             50912..51229
                     /locus_tag="ACHBKA_000053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9443785.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51259..51579
                     /locus_tag="ACHBKA_000054"
     CDS             51259..51579
                     /locus_tag="ACHBKA_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9443786.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51687..53147
                     /locus_tag="ACHBKA_000055"
     CDS             51687..53147
                     /locus_tag="ACHBKA_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9443787.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53215..53436
                     /locus_tag="ACHBKA_000056"
     CDS             53215..53436
                     /locus_tag="ACHBKA_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443788.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53467..53648
                     /locus_tag="ACHBKA_000057"
                     /pseudo
     CDS             53467..53648
                     /locus_tag="ACHBKA_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
     gene            53648..54061
                     /locus_tag="ACHBKA_000058"
     CDS             53648..54061
                     /locus_tag="ACHBKA_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM9443789.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54184..55365
                     /locus_tag="ACHBKA_000059"
     CDS             54184..55365
                     /locus_tag="ACHBKA_000059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9443790.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55436..55600)
                     /locus_tag="ACHBKA_000060"
     CDS             complement(55436..55600)
                     /locus_tag="ACHBKA_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443791.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55754..55827
                     /locus_tag="ACHBKA_000061"
     tRNA            55754..55827
                     /locus_tag="ACHBKA_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55788..55790,aa:Arg,seq:cct)
     gene            complement(55873..55986)
                     /locus_tag="ACHBKA_000062"
                     /pseudo
     CDS             complement(55873..55986)
                     /locus_tag="ACHBKA_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(56369..57601)
                     /locus_tag="ACHBKA_000063"
     CDS             complement(56369..57601)
                     /locus_tag="ACHBKA_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM9443792.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57855..58424
                     /locus_tag="ACHBKA_000064"
     CDS             57855..58424
                     /locus_tag="ACHBKA_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9443793.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58602..59042)
                     /locus_tag="ACHBKA_000065"
     CDS             complement(58602..59042)
                     /locus_tag="ACHBKA_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM9443794.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59200..59964
                     /gene="map"
                     /locus_tag="ACHBKA_000066"
     CDS             59200..59964
                     /gene="map"
                     /locus_tag="ACHBKA_000066"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM9443795.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            59996..60919
                     /locus_tag="ACHBKA_000067"
     CDS             59996..60919
                     /locus_tag="ACHBKA_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM9443796.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            60995..62134
                     /locus_tag="ACHBKA_000068"
     CDS             60995..62134
                     /locus_tag="ACHBKA_000068"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM9443797.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62127..62927
                     /locus_tag="ACHBKA_000069"
     CDS             62127..62927
                     /locus_tag="ACHBKA_000069"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9443798.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62927..63754
                     /locus_tag="ACHBKA_000070"
     CDS             62927..63754
                     /locus_tag="ACHBKA_000070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9443799.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63759..64466
                     /locus_tag="ACHBKA_000071"
     CDS             63759..64466
                     /locus_tag="ACHBKA_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9443800.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            64566..65432
                     /gene="rfbA"
                     /locus_tag="ACHBKA_000072"
     CDS             64566..65432
                     /gene="rfbA"
                     /locus_tag="ACHBKA_000072"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM9443801.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65446..66018
                     /gene="rfbC"
                     /locus_tag="ACHBKA_000073"
     CDS             65446..66018
                     /gene="rfbC"
                     /locus_tag="ACHBKA_000073"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM9443802.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66040..67068
                     /gene="rfbB"
                     /locus_tag="ACHBKA_000074"
     CDS             66040..67068
                     /gene="rfbB"
                     /locus_tag="ACHBKA_000074"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM9443803.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67166..68017
                     /gene="rfbD"
                     /locus_tag="ACHBKA_000075"
     CDS             67166..68017
                     /gene="rfbD"
                     /locus_tag="ACHBKA_000075"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM9443804.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68052..70085
                     /locus_tag="ACHBKA_000076"
     CDS             68052..70085
                     /locus_tag="ACHBKA_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443805.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70129..71409
                     /locus_tag="ACHBKA_000077"
     CDS             70129..71409
                     /locus_tag="ACHBKA_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM9443806.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71619..72425
                     /locus_tag="ACHBKA_000078"
     CDS             71619..72425
                     /locus_tag="ACHBKA_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9443807.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72437..73657
                     /locus_tag="ACHBKA_000079"
     CDS             72437..73657
                     /locus_tag="ACHBKA_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9443808.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73647..75233
                     /locus_tag="ACHBKA_000080"
     CDS             73647..75233
                     /locus_tag="ACHBKA_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9443809.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75272..77410
                     /locus_tag="ACHBKA_000081"
     CDS             75272..77410
                     /locus_tag="ACHBKA_000081"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9443810.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77778..78761)
                     /locus_tag="ACHBKA_000082"
     CDS             complement(77778..78761)
                     /locus_tag="ACHBKA_000082"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9443811.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79139..80530
                     /locus_tag="ACHBKA_000083"
     CDS             79139..80530
                     /locus_tag="ACHBKA_000083"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9443812.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80546..81586
                     /locus_tag="ACHBKA_000084"
     CDS             80546..81586
                     /locus_tag="ACHBKA_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9443813.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81605..>81742
                     /locus_tag="ACHBKA_000085"
     CDS             81605..>81742
                     /locus_tag="ACHBKA_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM9443814.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQ"
BASE COUNT        26665 a        13822 c        17330 g        23925 t
ORIGIN      
        1 gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt tgattatatt
       61 tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat
      121 tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc
      181 gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa
      241 aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttatacaaa
      301 agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata ttatcggtag
      361 tcccatcttt cagattccat atattggact agtcctttca aaaacggcat ttgcatggat
      421 tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat ggaatacctt
      481 tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat gttaaaaaca
      541 taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa aacactcatg
      601 cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt agtatgcgca
      661 atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg cgtacccagc
      721 aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta gcagaagtac
      781 cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag cttcagcctg
      841 aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg gccacgacaa
      901 tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc gagaaagcta
      961 aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa ggtcaagcag
     1021 cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat agtgcaaaat
     1081 caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt ggcgttttga
     1141 tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag attccccgtg
     1201 gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg gatcatcccg
     1261 atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca aaggaatcag
     1321 aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt gaatcgaggg
     1381 ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag gatacaggaa
     1441 atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat tgataacctt
     1501 agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg ggccaaaata
     1561 attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt aattatctat
     1621 aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag catccgcctt
     1681 ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg ttagaaactg
     1741 aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc aaaggcaaat
     1801 aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata aaactcatca
     1861 gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta gttgcaattg
     1921 aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg acaagtaggg
     1981 tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg tttaagaacc
     2041 aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat ctttcttccg
     2101 catctaaaga aatcacaagt atgctattaa tatctatttt ttttgttgca ttatatggca
     2161 gtttgactag cgatcgttgg attttactca aacaaggtgg gctagctctg ttggcattgc
     2221 tttctatttt cttataaata tagaaaaaat aagaatttat ctaacaggaa actttgtgaa
     2281 aaatattact ttatctcatg aaatatgata ttctaatgaa tggtatttta gaaaatttca
     2341 tgaaaaagga gttagatagg tgttcaaaaa ttatgtttcc tcactaaaaa aagaattttc
     2401 aggttatgat ctttcaagat ttcagaaaga tcttttggct gggatcacag tagctgctgt
     2461 agcattgcct ttagccttgg cttttggggt atcaagcgga gctgatgcgg ccgcaggttt
     2521 gattacggct atcattgccg gtatcgttat tggcggttta tcaggcggtt tttatcaaat
     2581 atcaggtccg acaggtgcga tggcagctat cttgatgtct attgcggcaa caaagggaat
     2641 gcaaggtgta ttgcttgcta cttttttagc gggtctattt ttacttgcag caggactttt
     2701 ccgattagga acactgactt cttttatccc agcacccgta attacggggt ttacttcagg
     2761 tattgctatc attattgcat taggtcagat cgataattta tttggtgttc actcggaagg
     2821 tacaaacgtt attgaaaaac taagtagtta tcaaacgata ggatttccga taaacacagc
     2881 ctctttgatc atgggagggc tagtcatttt agggatgttt atctatccaa aaaaatgggc
     2941 gaaacgaatg cctagttcgc tgctggctat cattttggca acagcagtta tgctattgtt
     3001 ccatttacca gtagcaactg tgggtaagat tccacaaaca ttgatcagta gtaatcgttt
     3061 gaatatggga gattttagct tttctgcctt gcaacaagtg gcagttccag ctattagcat
     3121 tgctttatta ggaatgattg aaagcttgct atgtggtgct tcggctggcc gaatggcaaa
     3181 taagccctta gatagcaatc aagaattaat ggcgcaagga attggaaatc tgttgcttcc
     3241 tttctttggc gggattcctg ctactgcggc catcgcccgt acaagcgtgg ccatcaaatc
     3301 tggtgcacaa actagattag ctggtatgat acatgcagtt gtcttactgc tctccatgct
     3361 tgtttttgcg ccagtgatgt cacagattcc aatgccagca cttgcaggtg ttttgattgt
     3421 gacggcttgg cgtatgaatg aatgggaaac gatcaaggag ctatttacta ataaatattg
     3481 gtcagcagtt ctattgttta tccttacgat gatttgtaca gttatctttg atttaagtat
     3541 tgctatcgtg atcggggtga tcggcggctg tgtatttttt gttgtcaaaa gcgctgctat
     3601 cacgatttca ttagaaacga tcgattggca aagaatgggg ctgccagaaa cggaaaagct
     3661 gaataactgg acagtagtgt atattagtgg tccactgttt tttatgtctt cagaaaaact
     3721 caaaaataaa ttggaagaac taaatcatca cgatggtgtc attttctcca tgcgaggcgt
     3781 accaagtatt gatttgacgg cacgtgcagt ttttgatgaa tttcaagaaa aagcagagca
     3841 aagaggacaa acgattatct atacttcctt gcagccagaa gtagaaaaac aactttctca
     3901 cttgtgggaa catacattca cgcatcaaca tcaaacagtt gctcatgcgt tgacagcttt
     3961 acatgcgaaa tatactgcaa ttagccatta aaacaatgaa aaagaacaga caagactgta
     4021 aactgaagat tttatggtct tgtctgttct ttttaatatt gctgtaaaag tgaagctagt
     4081 acttgccagt cttgatggat aacaggtaca agggagcgat tgtagaaaat agctgctgga
     4141 tgaaaaagtg gaagaattcg atagtttttt tctgaccatt cgtaggcatc cttttgttct
     4201 gttaattgta agacaggccc tgtatagagt tgaccatgat ttttggtaac cgtatattct
     4261 ttgccaagta aacgttgaag accaatgttt ccaagtgtcg caattaattt tggctggacc
     4321 ttcatcagtt cgtaatccaa tattggtgca tgcgcaaata cttcggaacg agtaggtgtc
     4381 cgatttggat aaacagtttc tgactgttgt gtccgtttat tgatccgatg agtcacgcga
     4441 tacgggcggc tccttacagc actggtgata tacacctctt ctcgtgttaa gccagttgaa
     4501 gaaagtgcct gcatcaattc ttttccagct tgaccgctaa aaggaacatg cgtatcaatc
     4561 tctgttttcc ctggggcttc tcccacgagc atcagtttag gatgaagagg tccttgccca
     4621 gcgataaatc cttctacagg acggccgttc attcttaaag aagcttcttg tatcagttct
     4681 tttgggtatt ccatttatgc tcctcctttt tcttttatta taagaataaa agaaacacaa
     4741 tgcgactatt ttatttggta gaactgtgag gatagaaaag cagaagtatt gcttgaattt
     4801 ttgttttact ttttgatttt aaatttagtt gactaacggt tgctgttctg gtaagctagt
     4861 actgttgtaa ttgtggactc cacagctaca accgctcaga acaagtaata agcacagcat
     4921 atgctgtcac ttcggatggc gagtctaagt atatcagaag gaggtgcttt atagcatgta
     4981 cgcaattatt aaaactggtg gtaaacaagt aaaagttgaa gtgggacaag caatctatgt
     5041 tgaaaaactt gacgttgaag caggagaaaa agttgttttt gacgaagtta tcttagtagg
     5101 cggagaatct acaaaagtag gcgctccaac tgttgcaggt gcaactgttg aaggtactgt
     5161 tgaaaaacac ggcaaacaaa agaaagtcgt tactttcaaa tacaaaccta aaaaacattc
     5221 acaccgtaaa caaggtcatc gtcaaccata tacaaaagtg gttatcgacg cgatcaacgc
     5281 ttaatagcgt ttgagaaaga aggcctatat atgatcaaag gaacatttaa gcgaaatgac
     5341 gcaggtcaga tcgtttcatt cactttgacc gggcatgctg atgctggtcc ttatggaagt
     5401 gatatcgttt gtgcgggtgt ttctgcgtta gcgatcagca ctgtcaacgg gattgcctca
     5461 ttagccggtt ttgagccgat tgttgaaatg aatgaagaag aaggcggtta tctttatacc
     5521 gaagtcactt caggaatgac acaagaacaa aacaatattg ctgagattct attagaaaat
     5581 cttttattag gattacaatc gatagaagct gaaaactcag aatatatcca aattaaaaca
     5641 attaatgata aataggaggt gcagaccatg ttgttatcta tgaatctaca attatttgcc
     5701 cacaaaaaag gtggcggttc tacatctaac ggccgtgact cagaatcaaa acgtctaggt
     5761 gctaaacgtg ctgatggaca aacagttaca ggtggatcaa ttctttaccg tcaacgcggt
     5821 acaaaaatct atccaggtgt aaacgtgggt atcggtggag acgacacttt atttgcaaaa
     5881 gttgatggcg tagtacgttt cgaacgtaaa ggccgtaaca aaaaacaagt gtctgtttat
     5941 ccagtagctc aagaagctta attgaatctt atgattaaag aggttgtgac aagacttttg
     6001 tcacagcctt ttttgtttat aaaaataaaa gttgtagtgg aggctacgaa gtttactttg
     6061 tctttttaca cttaatattt ttttgccaat ttggaatcag gatgttttga taactatcgt
     6121 tttcaattga tggaagctat attgtttgct atcagtgaaa taattatatg gtaaacagtg
     6181 ttctttttct tgtttccatt ataaaaatcg tatcgggatt tttcgttctt ttaaattgaa
     6241 catttctatc tatccgcgtg ttatcaacaa aacaaaatca ggtaaatagt tgacgaaaca
     6301 gaaataaata catagtatta tgttttgaag cctaagtaca atagtctata gaaaaggagc
     6361 gataccattg gcaaaacgca ttgaccgaat ttatatgtat gtcaaagaaa aaaccgctca
     6421 tttaacaaag gcagaatatg atcaaggagt aacaacacag gaaattgctg aagtgttagg
     6481 gattcagcga acaaatagca gtaaagatct gaatcagcta gtgcgcgaag gaaaactgat
     6541 aaagacagac ggacgaccag tacgttacat atatcaaaca ctcgttacgc acgtaaagcc
     6601 agttacaaaa catgtagtca gttacaaaga aacgccggtc tctgaagaaa aaaatacact
     6661 ccaaatagat actaaggata tctttgcaaa aatcattgga gctaacggca gcatgaaaaa
     6721 ttcagtggaa caggcaaaag ctgcgatttt gtatccgcca agaggattaa attgtttgat
     6781 tacaggacct accggatcag gaaagactta ttttgcacac gcgatgtatc attttgcaaa
     6841 agcgcatcat gtaatagatg aggaaacgga actgattgtt ttcaattgtg ctgattatgc
     6901 caataaccct gagcttttga tgagtcattt atttgggtac gcaaaaggtg cttttacagg
     6961 cgcagatgaa gaaaaaatgg ggattatcga tcaagcggat ggcggtatgc tgtttttgga
     7021 tgagattcac cggttgccgc ctgaaggtca agaaatgatc ttttacttta tggatcatgg
     7081 aacttacagt cgtcttgggg aaacaacgaa atcccatgaa gcaaatgtac ggatcgtagg
     7141 tgcgaccaca gaagacccag gttcttctct attagaaact tttgttcggc gtatcccaat
     7201 caatatcaaa cttccggctt ttgaaaaacg tcctgcaaat gagaaagtcg atttagtaaa
     7261 aatcatgatt gcccacgaag caaaccgcac gcagcgaaag atttctttga cagaagatgt
     7321 agcaaaagca ttgatcggca gtgtgaccta tggaaatata ggacaattga agtcaaatat
     7381 ccagttagta tgtgcacgag gatttctaaa tcatatgcaa tcacccgaaa tctcgatcac
     7441 aatcgatgat ttgacagaag ggattcgtag cggattgatt caattagcaa gcaaccgcag
     7501 tgcaatggca gagctttcta aaatattgga gccaaagata actgtagcac caaatgatac
     7561 gatcatgaaa atccaatcgg actcctatga gttaccttat aatctatatg atatcatcgg
     7621 agataaagca gctttactga agtcggatgg tttggatcaa gaagcaatca atcatttcat
     7681 ttctacagat atcaacatcc atctgaagtc tttctataaa gaccatggtt tttcttttaa
     7741 tgcagacagc aaattagcag aatttgtaga tccgaaaatc atagaagtga ccaatcagat
     7801 ttatgtaatg gtcaaaagat ctttaccata tgagtttcag caaaacttta tttatgctat
     7861 gagcttgcat atcagttctt ttttgaaaaa aatcaactta ggagaagaac ggcatacgaa
     7921 tgataacatc cgtgagatgg cttttgattt tccagatgaa tatgctgttg cgaaagaagt
     7981 gcgtagttat atcgaaacgt attttcaagt ggagattcca gaaagtgaag actattactt
     8041 gaccgttcta ctcgtctcgt tacgggcaaa tcaagcaagc ggcagaatcg gtgtagtcgt
     8101 tgcagctcac ggaaacagca cagcaagcag tatggtccaa gttgtccaac agttgttaga
     8161 tgcagagaac gtacgagcag tagacatgcc tttggatatg gatccgaaaa cagcattgga
     8221 ccgaattgaa cgcaatgtac aagaagtaga tgaaggaagc ggggtcatac tcttagtaga
     8281 tatggggtcc cttgcgtcgt ttaattcgca aattcaaaga gacactggaa ttccagtacg
     8341 tactgttgat atggtaacaa cttctctagt tttggaaact gtacgaaagg tctcagtatt
     8401 agggacagat ctagatatgt tgcatgaatc attgaaaaat tttcgaggtt acgcagaaat
     8461 atcttctgta gaatctgttc aggaagcaaa acctattcat aagaaaaaag ctgttttggc
     8521 tatctgcgca tctggcgaag gtacagcaca aagaatcaaa gaattgatcg aacgagcggt
     8581 aaacaagcgt caagaaacag aattagaagt tctagctttg tcgattgtgg aagtgaaaga
     8641 agaactgcca gttatccaaa aagaatatca gatcatagct actactggga ttactgattt
     8701 gaatatccaa gccccattta ttcctttaga acgttttatt gatcaaaata tcgaagtcat
     8761 tttggatcag ttgctgatgg aatcagaatt ggaggaaact gaattcatct ccctagatga
     8821 agagtcagct aaaaatacgt gtgttgagtt tatctctgat aacttcatct ttattaatgg
     8881 tagtaagtta atcgatccaa tgtggcaatt ttctacacaa atctctcaga cgacgggaat
     8941 cggcgatgaa gaatatggat tcaaaatcaa cctagtgatg cacacagcag gaatgatcga
     9001 acgtattatt cggaatgaac cattgactgt ggaagaaaac gaattgacaa atacaacgaa
     9061 tgatcctctt tatagccaat tggcagcctc agttgttctg ttggaagatc agataaaagt
     9121 aaaagtgccg atagaagaaa tgtattatct gctacgtcta gtccataatc agttagataa
     9181 aaaagaatac actgtgccat gaatacacaa aaacgataca cttttatagt gtatcgtttt
     9241 tgtgtattgg ttaagaatcc tttaaattca attagtgtat cgtaaaaaag ttggcacgct
     9301 ttttgcttat agtagtgtgt gctaggaggt ggttgttcac acagaaatga taggggtttc
     9361 tgtgaagtag tgaggaagaa ggagttttgc agatggatat tcgattggca cgtatagatg
     9421 atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa gcaaacaggt gttgaacgta
     9481 tcattgtgat cagcgatgta gtcgctcaag ataaattgag aaagtttcta ctaaaagaag
     9541 ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca gaaaatgatt caaatctatc
     9601 atagtggatt attatcacga gcaaaagtga tgcttttatt caccaatccc caagatgttg
     9661 aagcggttgt ccgctgcggt gtacagttga agtcgttgaa tatcggcggt atgagtttta
     9721 ctgaaggcaa atcaatgatt accaattttg tttcagtaaa tcaacaagat attgattcct
     9781 ttcgttactt gaacagtcaa gaagtggagt tagagattcg aaaagtacct gctgacagga
     9841 aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt gtagcaagaa tggttgttgg
     9901 aaaaggtcaa ataattctgt tgtaatggtt agcaattatt aatatttttg aatttgttac
     9961 aacaaaagta aataggaggt ataaatatgg tagggattat cctagcaagt catggacaat
    10021 ttgctgaagg aatcttacaa tccggttcaa tgatctttgg cgaacaagaa aacgtcaaag
    10081 ccgtgatttt gaaaccaagt gaaggtccag atgacttgag agccaaattg gaagaagcgg
    10141 ttgcttcttt tgataatcaa gacgaagtgt tattcttagt tgatttatgg ggcggaacac
    10201 cgttcaatca atcaaacaca ttattcgaag agcacaaaga taaatgggca atcgtcagcg
    10261 gtttgaattt accaatgttg attgaagcct acgcatcacg cttttcaatg gaatctgctc
    10321 atgaaattgc agcacacatc attgaaacag caaaagacgg tgtaaaagta aaaccggaag
    10381 aattagaacc ggcagaagca ccgaaagcag cagttgaaga cgcacaacct aaaggagcac
    10441 ttccagaagg aactgtggtc ggagacggaa aaatcaaata tgtattagca cgtgttgact
    10501 cacgtctgtt acatggacaa gttgcgacag cttggacaaa agcagtgcaa ccaaaccgta
    10561 ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg taagagacta atcgaacaag
    10621 ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag caaaatgatc gaagtagcaa
    10681 aagaccctcg ttttggaaat accaaagcat tgcttttatt tgaaaatcct gaagacgtat
    10741 tgacagctgt tgaaggtggc gtagacatca aagaattaaa tgttgggtca atggcgcatt
    10801 cggtcggtaa agtcgttgta agtaaagtat tgtctatggg acaagaagac gttgaagcgt
    10861 ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg taaagtacct aatgattcac
    10921 gcgataacat ggatgatatt ttgaaaaaag caaaagctga attagcaaaa gcgtaataaa
    10981 taatattaaa atttatagga ggcttatcat gtctattatt tcaataattt tagtcgtatt
    11041 agttgccttt ctagctggta tggaaggtat tcttgatgaa tttcaatttc atcaaccact
    11101 agtggcatgt acattgatcg gtttagttac cggtaacctc gaagctggta tcgtacttgg
    11161 cggtacgctt cagatgatcg ctttaggttg ggcaaatatc ggtgccgctg tagcaccaga
    11221 tgctgcatta gcatcagtag catcagcaat tattttagtt ttaggtggac aaggtgttaa
    11281 aggtgttcct tctgcgatcg cgattgccgt tcctttggct gttgcagggt tgttcttaac
    11341 aatgatcgta cgtacaatcg ctgttccaat cgttcatttg atggatgcgg ctgctgaaga
    11401 aggaaacatc cgtaaggttg aaatgtggca catcattgct gtatgtttac aaggggttcg
    11461 tatcgcggtc ccagcagcag cgttgctatt tatcccagca caaactgtgc aatcattcct
    11521 tgaatcaatg ccagcatggt taacagatgg tatggctatc ggtgggggaa tggtcgtagc
    11581 tgtaggttac gctttggtaa tcaacatgat ggctacaaaa gaagtatggc cattcttcgt
    11641 tattggtttc gttgtagctg caatttctca attaacattg attgctctag gtgctttagg
    11701 tgttgcatta gctcttattt acttgaacct ttctaaaatg ggtggttcat caaataatgg
    11761 aggcggaagc aactctggtg accctttagg cgacattttg aatgactatt aatcttgaag
    11821 gaggaaaata aaatggcaga agaaaaaatc aaattatcaa aaaaagatcg tttagctgtt
    11881 gcgtggcgct ctaccttcat tcaaggttca tggaactacg aacgtatgca aaacggtggc
    11941 tgggcatttt ctatgattcc agcaatcaaa aaattataca aaacaaaaga agaacgttct
    12001 gcagcattaa aacgtcactt ggaattcttt aatacacacc catacattgc ttctccaatt
    12061 ttaggggtta cattggctct ggaagaagaa cgtgcgaatg gcgcgcctgt tgatgatgta
    12121 gcgattcaag gggttaaagt aggtatgatg ggtcctttag ctggtgttgg tgacccagtg
    12181 ttctggttta cagtacgtcc aatgctaggt gcactaggtg cttcattagc tatgggagga
    12241 aatatccttg gaccaatcat cttcttctta ggttggaaca ttattcgttg ggcattcatg
    12301 tggtatacac aagaatttgg ctacaaagct ggttctaaaa ttactgatga cctttctggc
    12361 ggattgttac aagacgtaac aaaaggtgct tcaattttag ggatgttcgt attagctgcc
    12421 cttgtacaaa gatgggtatc aatcaaattc ttaccagttg tatcaactgt aaaattagat
    12481 aaaggtgctt acatcgaatg ggataaattg ccttcaggtg gtgaaggaat caagagtgca
    12541 tttgaacaag ttaataatgg cttagccctt tctcctgaaa aggtaacaac attacaagac
    12601 aacttagacc aattgattcc tggtttagct gcattaggat taacattgtt atgtatgtgg
    12661 ttgcttaaga aaaaggtttc tccaatcgtt atcatccttg gtttattcgt agtcggtgtt
    12721 gttggacacg tcatcggact tttataatcc ataaaaatat agctcgggcg ttgtcccgag
    12781 ctttttttat atagtatact gttcataaac aaatatctgg agggagaaaa tggttcaatc
    12841 aatcaataca aaagtagact tggtaattga tgctacttcg tttacgggtc tgacagatta
    12901 cgggcaaatc atgataggtg ataatggttt tgagttttac catgcgcgag atagacgcaa
    12961 atatatccaa attccatggg aagaagtgga ttatgtacta gcatcagtca tgctaaaggg
    13021 aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat ggtacataca cattttcttc
    13081 caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat gtagatgaag atagaatggt
    13141 acaatcattg agtttctttg atgtagtgaa acgtggtatt cgtagtatgt ttaaacgcaa
    13201 gtaaaaaata aagaaaactt ctaagaggag gttttcttta tttttttact ttgcagaaga
    13261 aaatatgaaa tagcaaaacc aattatttgt cccctttaaa tcccatatgc taaaattgat
    13321 tagcaaagaa ggaggttata ttatgagttg gttatggaca ttgattgttg gagccgtgat
    13381 cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg taacgaatat
    13441 cattgctgga ctagtcggtt caagtattgg acaagcgatt ttcggcgaat ggggaccaaa
    13501 agctgcaggt atggcactta ttccatctat tttaggcgca gtgattttgg tagcagttgt
    13561 ctcatttttc tttggaaaac gatcataagt ttttacaaaa tagtaaacta aaaggagttg
    13621 cagcgagact gtgactcctt tttggcttta tttatttaaa ataaaaagaa gttttccgga
    13681 aatttttatt taactgtcgt gtacgaagaa aagtttgcta taatggaaaa aagcgtgcaa
    13741 agaaagaggg agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa aaatgcaaga
    13801 agaaaatttg gattcatttt tggttaccag tccgtacaat cttcgttact taacgaactt
    13861 tacaggtacg acaggattag ctgttattac tttggaaaaa gcatttttta ttactgattt
    13921 tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt gaaattatta aaaatgtcgg
    13981 acctattttt gatgaggtag caaacttagt acgcaaagaa gaactgcact cattagcttt
    14041 tgaggaaaca acagtctcat ttttagaata ttcggtttta gaagaaatca tcgatgcgga
    14101 attagtacct gtttcacaga tgatcgaaga actcagagaa gtgaaagatg aagaagaaat
    14161 cgctattatc gaaaaagcat gtcatattgc ggatatggca tatgatcaca tcctgaaaat
    14221 gatccaaccg ggcatgactg agattgaagt cgcaaatcaa ctagactttt atatgcgttc
    14281 tcttggcgct tcaggtgttt cttttgatac gatcgttgct agcggattgc gttcggctat
    14341 gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt gacttgatca cattggactt
    14401 tggttgctat tacgaagggt acgtttccga tatgacacgt acattcgcta ttggtgatcc
    14461 tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat gctcaattag cagttattga
    14521 tgctgcaaaa ccaggaatga ctggtgtcca attagatgca gttgctagag attatatttc
    14581 aaaacatggt tacggggaag catttggaca ttctacagga catgggatcg gtttagagat
    14641 ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag gaaatgtgat
    14701 cactgatgaa ccagggatct acttaccagg aatcggtgga gtacgtattg aggatgactt
    14761 gttgatcaca gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat tgatcatctt
    14821 atagtttcat caatagaaga agtaagcgta aaaatcagtg ttcacagact ttgttataga
    14881 aaatcactcg atttaatgat acactataga agaactttta agctgtaaga gataataaag
    14941 ttgggtgttt tctccaattc atagcagttt gcagctgatc ggcggcagtt ttcttaccac
    15001 gtgatcacta tgccgcatca tagagaattc ggatgtatct cgtgcttagg caaagggagg
    15061 aataaataaa tggcagatga aaaaaatttg attttaaacg caaaccaaga tttaggagag
    15121 atcgtgatcg ctcctgaagt catcgaagta attatcggga ttgcagcttc taaagtagaa
    15181 ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt aggacgtgca
    15241 gctcacggaa aaggtgttta cttagcaaac gaggaagatg gcttgaaagt agatctttac
    15301 tgttatctag aatatggtgt ttcagttcct aaagtagcaa tggaaatgca ggaacgggtc
    15361 agccagcaag tccttttcat gacggatatc gatttatctg aagtcaatat ccatgtagta
    15421 gctgttgttc ctgaaaaatt agaacaacca acaatcgaag aactttttga agacgaagag
    15481 gaagagaatg aataaagaat tatcaagaca cgagattcgg gaaatggcat tgcaggcttt
    15541 atttccatta gacttcaatg cagacctaac aaaagaagat gcgattttca acgcaatcga
    15601 attagaccac cgcgacatga tcaacgaaga tgaatcagag tttgttcctg tttatcttga
    15661 tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga ttgaaaaaca
    15721 tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg gatttggtga tcttgcgtat
    15781 cgcgattttt gaaatgactt atgtgacaga tgtaccagcg gctgttgctt taaatgaagc
    15841 aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca acggtgtttt
    15901 atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt tctatgaatc
    15961 atcataaacc tgttataaat cgggaaaatt ctatggtaga atgaaaacac aaaaaatatc
    16021 tgggagtggt tagatggcag aattgattaa cggaaaagaa ttagctgaaa aaatgcaggc
    16081 agagattgct gaaaaaatcc aaaaattgaa agataatggt atccatcccg gattagtcgt
    16141 tttattggta ggggaaaatc cagccagcca aatctatgta agaaataaag aacgtgtagc
    16201 aaaagaaatc ggaatccatt cattggtaga gcgttatcct gagacgattt ctgaagacga
    16261 actacttgca gaaattgaaa aatacaatca agatcctagt tttcatggta tattggtcca
    16321 attaccattg ccaaagcata tagatgaaga aaagatcctg ttagctatcc gccctgaaaa
    16381 agacgtagac ggttttcatc cgatgaattt aggcagactc cttgcgggta aaccggacaa
    16441 gattccttgt actccatacg gcatcatgaa aatgtttgaa gcttattcta tcgatccggc
    16501 gggtaaacga gcagtagtta tcggacgaag caatatcgtc ggtaaaccga tggctcagct
    16561 actcttgatg gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa atctggctga
    16621 actggcacaa gaagcagata ttttagtcgt agcaatagga cgtggacatt ttgtcacaaa
    16681 agaattcgta aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg atgctaacgg
    16741 taagctgatc ggtgatgtga aatttgatga agtggagcct attgcaggct ggatcactcc
    16801 tgtgccaaag ggtgtagggc caatgactat cacgatgctg atgtatcaaa ccgtaaaatc
    16861 tgccgaagct tttggagcag gtgaatagat ggcacaagaa tatttaactg tcactacatt
    16921 aacgaagtat ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg tatatttaac
    16981 aggcgaaata tcgaatttcc gtttacgtgc caatgcccat caatatttca gcttaaaaga
    17041 tgaccatgca aaaatatctg ctattatgtt caaaggagca ttccaaaaat tacgattcca
    17101 gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg aagcgggagg
    17161 ttcttatcaa atctatgtag agcacatgga gcctgatgga ataggtgctt tatatcaaga
    17221 attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc ctaaaaaaat
    17281 gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag cggttatccg
    17341 cgatatcatc acgacggtga aaagaagata tccaattgtc caattggttt tgttcccaac
    17401 agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg aatatccaga gagtagaaga
    17461 gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg aggatttatg
    17521 gccgtttaat gaagagcggg ttgctcgggc gatttatcaa gctaatacgc cggtgatttc
    17581 atctgtagga catgaaacag ataccacgat cgctgattta gtagcagatg ttcgggctgc
    17641 cactccaaca gcagctgcag aattagctgt tcctgttttg tcagaagagt tgatgcgcat
    17701 tcaagaacgg caagctagat tgcagcaggc gtttgtacgc cagatccagc gcaaacaaga
    17761 acggttcgag cgtgctcgac gttcctatgt atttcggcag cctgaaagat tgtatgaagg
    17821 tcagaccatc aaattagacc agttgaaaca gcgattatat caagctacac agcaaattta
    17881 tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag tggctccgct
    17941 ttatcgagtt aaagcagctc gccaagaaac tgcttatcta gaaaaacgct tagaagagaa
    18001 aatccaacaa tatatgaaac aacagcagca gcgttttgct caagctgttc aatctcttga
    18061 tttgttgagt ccattgaaaa tcatgggaag aggctacagc tatacgacgt taaatgatca
    18121 agtagtaaaa agtgtcaccg agattcatca aggggatttg ctgacgatcc attatcaaga
    18181 tggaatcgtt aaaggacaag tacaagagat agacaaagga gaataaaaat atggaaaatc
    18241 caacttttga agagtcactg caagaattag aaaaaattgt catgcagtta gaacaaggag
    18301 acgttccatt agaatcagct ctagattcgt ttaaacgagg aatggaatta agcaaacatt
    18361 gccaagatac attagctaaa gcagaagaga ccttgacgaa aatgatgaca gaatcaaatg
    18421 aagaagttga ttttgacgga aacgaggaaa gctgagatga gattaactga cttttcagct
    18481 attcatctac ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca tacaacggat
    18541 agaggactct atgatgcgat gagttattca gtgaaagcag gcgggaaaag aatacgtccc
    18601 ttattacttt taacggccgt tgcttccttt gatgagccta ttgatgttcc cgtctatcaa
    18661 gtagcagctg ctttagaaat ggtgcatacg tattccttga tccacgatga tttgcctgcg
    18721 atggacaatg atgatcttcg tagaggaaaa cctaccaatc ataaagtatt cggcgaagca
    18781 ttagctatat tggccggaga tggtttgctt acaggagcat tccagctaat cagtatggca
    18841 cacttaggta attctcctaa gcttctgctg cttcagcaat tagctgtttg tgcgggatca
    18901 caaggtatgg ttgctgggca agctgcagat atcgagggcg aatcgaaaaa gctttcttta
    18961 gaagaattag catttattca tgaaagaaag acagggcatt tgattcgtta tgccttgctg
    19021 gctggtggca tattagcgaa gcaacctgaa gaaatcttat tgctacttca acgattagcc
    19081 gaacatttag gtttagcttt tcagataaga gatgatctac tcgatgtgat tggtacaacg
    19141 aaaacattag ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac ttacccgcgt
    19201 ttattaggcc tagaaaagac acgtgaggcg ttggagattg agctgttatc cgccaacaag
    19261 atcatagaca aattagaaga ggacgttttt tcttttgatg gcgggctgat gcgccagatg
    19321 atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc gtagatgtat
    19381 tagcggtcaa acaaggatta tttgaaacaa gagaacaagc caaacgttca gtgatggctg
    19441 gtttgattta caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa attccagtga
    19501 gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg ggattaaaat
    19561 tagaaaaagc actcaaagca tttgatctat ctgttgaagg aaaaacattg ttagatattg
    19621 gtgcatccac tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa atgagttatg
    19681 ctttagacgt tggctacaat cagctagctt ggaaaataag acaagatcct agagtcgtcg
    19741 taatggaaag agtgaatttt cgttatgcaa aacccgaaga ctttacctta ggaattccag
    19801 aagttgctgt tatcgatgtt tcttttattt cactaaaact gatgctgcct ccattacatg
    19861 ctattttaaa agaagaggga gaagtgattg ctttaatcaa accccaattc gaagcaggca
    19921 gagaagctgt agggaaaaac ggaattgttc gtgaccctca gacccatcaa aaagttttag
    19981 aagacatttt gtcatttgcg gcacaaggtg gatatgatgt gctggaatta agttattctc
    20041 cgatcacggg gggagaagga aatatcgagt tcttggctca cttgaggaaa gtacccgaaa
    20101 gcggaacaat caattcagct attaatatgg cagaagtggt ttcaaatgca cacgaacagt
    20161 ttgaccataa ataaattgta actgtgaaaa tcaagtgaga agtatgcgga ttcttcttta
    20221 ttataccaag gaaataagct atcattgatt aagaagaata tgctaaggag cgaagacgta
    20281 atgagaaaaa aagatcggca tcgtttgatt actcgattgt tgagtgagca agatatccga
    20341 aagcaggaag aatttgttga aatcctgaaa agcaaaggaa tatctgttac tcaagctacg
    20401 atttccagag atataaagga actgaaactg atcaaggtac cggcttcaga tggcggctac
    20461 cgttatagca taccagctga aacaaacgaa gatgtaaata ctaaattgga aaaactatta
    20521 aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa aacattgccg
    20581 ggaaatgctt cggcagcagc aaatttgatt gataaacgtt acaagaaaga attattttcg
    20641 attatcaatg atgacgataa tgttttgatg attacacgaa ctgaagacca ggcagtttta
    20701 ttgaagaaag acttcctgca ttacctttaa gttgaggagc gaatgtgatg ctacaagaaa
    20761 tcagtattac taactttgcg attattccgg aattgcggct ttcttttcac gaaggaatga
    20821 ccgcgctgac cggagagact ggtgcgggta aatccattat tatcgatgct ttaggtctgt
    20881 tagcaggagg aagaggttca agcgattata tacgtcaagg agcagaaaaa tgtgtgcttg
    20941 aagggttgtt tgaactacca aaacaagaag gattctctga actgatggtt gaattaggga
    21001 tcgaaacaga tgaggacaat ctgattgttc gacgggatat gtccttaact ggaaaaaatg
    21061 tttgtcgagt gaatggtcat attatcacat tagctaatct gagaagaatc ggcagttatt
    21121 tagtagatat ccaaggacaa aacgaacatc aggaactgct ccagcctgaa tcacatcttg
    21181 ctttattgga tcgttttgga gatgtagctt tccaacagaa gaaaaagagt tatcagcaag
    21241 aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa aatgaaaaat
    21301 cctatgttca gagaatagat atgctccatt tccagcaaga agaaatagct gctgctgatt
    21361 tgcaagtcgg agaagaagag aaattaaaag aagaaagaga aaaacttagc aattatcaaa
    21421 aaattgctga tggattagct gcaggttacg gagcactgac gaataatgaa caaaacagtg
    21481 tagatggtgt tggcttggcc gtttcagaac tgcaagggat cgcccatcta gatgtggagt
    21541 atgaagcaat ttatgaaaat atccaaagtg cttactattt gctgcaagat gcaatcggcg
    21601 atatgagtcg tcagattgat ttattggagt tagatgagag tcgtttagaa gaagttacgc
    21661 aacgtttaga attaatccga cagttgaaac gtaaatacgg cgaatccatt gagtcaatat
    21721 tggcttacta tgacgaaatc accgaagagc tagcttcttc tgatttttca gaaggtcaat
    21781 tggacaaaat gaaagaggaa ctagagcaaa aagagcttct acttcagcaa caagccgcag
    21841 atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt ctgcatgaat
    21901 tgaaaagtct gtatatggaa aacactgagt ttgaggtacg tttcttaaaa gaagaaaata
    21961 gacaattgaa tcatgatgga tttgaccaaa tcgaatttta cattacgacc aatccaggag
    22021 agccactgaa gcctttggtg aaagtcgctt ctggtggcga actgtctcgg atgttgcttg
    22081 ctttgaaaac tattttttct tctgagcaag gtgtgacaag tattattttt gatgaagtgg
    22141 atacaggtgt gagcggaaga gttgcacagg ccatcgcaga taaaatttct caaatatcga
    22201 aatactctca agttttgtgc atcactcatc tgcctcaagt tgctgcagtc gccgactatc
    22261 aatattatat tgtgaaagca gtgattgatg gacgtacgca aacttctgtt tcagagttga
    22321 aaacaaaaga aagagaagaa gaaattgcac gtatgctggc tggaagtgag attacaaaac
    22381 tcacactcga acatgcaaaa gaactattga aactcgcaaa aaggacctga gccacctcgc
    22441 agtggttcag gtccttttca gcagctgata gctatatttt atttttacat gacactcatt
    22501 acagcagtca atacaacaga tccaatcgtt aaaatcagca ttgtccaaat gacaaatttt
    22561 gttactttag caaaagtact tgtttgtttt ttttcacgca ttgttttcaa cctcgtcatt
    22621 aattagttga ttcaagtgta caatcttttt tccatttcca caagagggct tttttataaa
    22681 aacttccctt tttttaaata ataggtagtt acgatacaaa taaataatgt taatagaact
    22741 agccatataa aggcatcatc cctttcagca ataggtaact taatgttcat tccatagata
    22801 ctgccgacga ttgtcggtat agttaaaata atcgttaaag aagttaaaat tttcataata
    22861 ttattcagat tattcgatac gattgctgaa aaagtctgat tcatttgttc tactagtttt
    22921 aattgaatgc gagaagtagt cattgcctgc tctgtttcca ccaatacatc acgtaaatga
    22981 tttttcaaag catgattttc ttggaaaagt ttattttgcg ctagttcttt caatgcatct
    23041 aaattagcag aagtcgcttc tctgaaataa acgaggcttt tttggatatc catgatttga
    23101 taaagttgtt tgttttcagt ggatacttga agtttggctt ccaaagtatc gcattgagat
    23161 tgaagcgtag ctaaataact gttgtaagag agtgtgacct gccaaatgaa ttgaaagaag
    23221 atattgagat taggcgaaat atcacttttt ggaaaggtct ggctaaagac tttctttaaa
    23281 aacagtggtt catgattcgt aattgtgata agcttctttt tgggcgtcaa aataatagag
    23341 agcgggtatg tatccacttg aaaataaccg ctaggacttg ttttagcaaa aggatattgt
    23401 aacaataata acgctggctt tgtcaacttt gattgattga attcttctgt acgggaattt
    23461 tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa tttcaaattg atgtgtcagc
    23521 tgatgaatct cttcttgatt aggacgttct acacaatacc agatcgtttg gtcagtttcc
    23581 tttccggtat tacgcagttt ctcattatct aatgtaaaat atttgatcat gattttcctc
    23641 ctgttgcctg tttcttttat tataacggat agggaattgg aatacatgga atgtgtttgt
    23701 aagatataga atataactgc ataaatttaa ataaaatgaa acagttgtgt aaagtatata
    23761 cagtatactt gaagcaacga taaaaaagga gtttacgttc atgggcacat tcacaccact
    23821 tatcttgttt tggtatattt acccggtaat cgttttgttt ggttgccaat ttcttgtaaa
    23881 attattttcg ttaaatcgtc gttttaaaat aaaagcccct gatttggcga ttcctttttt
    23941 gtggggcggt atgcatgcat tgtctagaaa tacagggaca atttctatct taccgttttt
    24001 ctttatttct gttttgctaa tgggaatttg tattgctgtt ttccaagcat attattatga
    24061 agaaattatt tatccgcgtt atttcaagat gacttggcgt ttggtttttc tgttgacaat
    24121 cgtcttgtac gctgtcttga tcattgtgaa catactttct tacctatgaa aaaaatagaa
    24181 aagctgtaaa aagtctttta aaaggctttt tgcagcttga tttttaaaaa aatgtttatg
    24241 aataaaaaaa gttgttcccg gatttcccag aaaaggtggt agaaagtggg ggattgtggt
    24301 agactaattt tagttagtgg aggaatgggg ggctttcaat gttgatgggt gaattccaac
    24361 ataatattga tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg
    24421 aaaagtttgt tctgacacgc ggattggatg gctgtctttt cggctatccg atgagcgaat
    24481 gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt
    24541 ttgtgcgatt cttctattcg gctgccactg aatgcgaact tgataaacaa ggacgaatca
    24601 atatacccag cacgttaaga aattatgcag ctttgacaaa aggttgcgta gtcatcggtg
    24661 tatctaaccg aattgaaatt tgggatgaga cacgatggca agatttttct gcagcagcag
    24721 aagagaactt tgacgaaatt gctgaaagta tgattgattt cggactataa gaataggaag
    24781 aggctgacta atgactgaaa catttcaaca ctatacagtc atgttgaaag aaacggtaga
    24841 tggattgaac atcaaaccgg atggcgttta tgtagactgt acactcggcg gtgccggaca
    24901 tagtgaatat ttattaagcc aactaagttc taaagggcat ctgtatgcgt ttgaccaaga
    24961 tcaaaaagca atcgatcacg ccaagctgcg tttagcttct tatgtcgaac aaggtatggt
    25021 cacatttatc aaagcgaact tcagagaatt agaagaacgc ttgagtgagc atgtagataa
    25081 ggtagacggc attttgtatg atctaggggt ttcctctcct cagctagatg aagcagaacg
    25141 tggatttagt tatcatcaag atgcaccgtt ggatatgcgc atggatcagt cagctccttt
    25201 atccgcttat cacgtaatta atgaatattc ctaccatgaa ttagtaaaga ttttcttccg
    25261 ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac
    25321 aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc
    25381 cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt caagctgtgc ggattgcagt
    25441 aaatgacgaa ctcggtgccg aagaggcctc tttagaacag gcaatccggt tattgaaagt
    25501 caatggaaga atcagcgtaa tcacattcca ttcattggaa gacagaattg tcaaaagtat
    25561 gtttaaagaa tatagtacgg tacaagatct accaccggga cttcctgtca tacctgacga
    25621 attccaacca gaactgaaag tcattactcg taaaccaatc cttccttcag aggaagagct
    25681 aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa
    25741 agaggagtag aatgatggca gaattaaaga aaatgcaaga ttacgaatat gatttacctg
    25801 aattggtgga tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc
    25861 ttccacaatc accggcaaaa cgattaaagc agatttctgc tttggaaaaa attgctgtgg
    25921 caagcgtggc gattgctatt gttgctttat gtgttttaac agttatgctg cgcacaaata
    25981 tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag
    26041 aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg tactgaacgg attaaacaaa
    26101 tcgcagaaga aaaaggattg tctattaatg acgacaattt aaggaaagtg aagtaaatga
    26161 gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg atgaacaatc
    26221 gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg attgttcttt ttatttgccg
    26281 tccgtttctc gtatatcgtt attggaggtc atgttgctgg tacctcatta gctgaaaaaa
    26341 caaaacagtt gtaccaagga agtgaagtag taaaagcaaa aagaggaacg atttatgatc
    26401 gtaacggtgt ggcgctcgca gaagatgcct cctcttattc aattaaagcg atactctcaa
    26461 aaacatatac ctctggggat aaaaaactgt atgttgaaga aaagaatttt gataaaatag
    26521 cagaaatcct gcataagaat ctttctatag acaaaaaaga tgcactgaat attttagagg
    26581 acggcgcaaa aaaagagctg tatcaagttg aatttggtag ctatgggaaa aatatctccc
    26641 aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt
    26701 ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt ttcttctcat tttattggat
    26761 acgcagttcc agacaaagat gagaatggct tagttggtaa actaggtttg gagtctgctt
    26821 acaatgatat cttgagcggt aaagatggta agattattta tcaaaaagat aattttcaaa
    26881 atcctctgcc aggtacagtg gcagaagaag aaaaagctgt cgacggacaa gatatctaca
    26941 cgactttaga cagcagacta caaagttatt tagaaacact gatggatcaa gtaaatgaag
    27001 aatatcagcc agaagaattg acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg
    27061 caatgggaca acgacctact ttcaatcctg aaacaatgga aggattgact ggaaaagatg
    27121 ctatctggcg taatttcttg gtacaagata gttatgaacc aggatcaacc atgaaagttt
    27181 tcactactgc agctgctatc gaagaaggag aattcaacga gaatgaaacc ttccaatctg
    27241 gaaaaatcca agtagaagat gctacgatca atgaccatga ctttggtgaa aaaggtgtgc
    27301 tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc
    27361 gattaggcgg acgatggtat aactatttac aaaaactggg atttggacaa agtactcatt
    27421 ctggattaga tgatgaagta aacggtgctt tacctacatt aaatattgtc gaccgagcaa
    27481 tgagtgctta tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa gctttcactt
    27541 ctattgcgaa taacggtaca atgattcaac ctcgatatat cagcaaagtg gtggatcccc
    27601 aaactggaga agagcgaaca actcaaacag aagttctagg gcaacctttt tcaaaagaaa
    27661 caacagaaaa agtccgtgaa tatatgagag atgttgtcga aagcgaaaac tacggtagtg
    27721 cttatggtgt atatagtgta cctggataca atgtatcggc aaaaaacgga acagcccaaa
    27781 tcgcttctga tacaggggga tatcaaacag gagatacagc ctacctgtat tcgattgtgg
    27841 agatggttcc ttctgaagat ccagactatg tcttgtatct aacaatgaaa caccctaaga
    27901 catatgatcg aatggcattg gctaaaatcg caaatccatt gatgaaacgg gcaatggatt
    27961 ttaaagaaac tgaagaagat tcagatacag aaacaaaaac agaaaaagta tccgtagccg
    28021 attatcgtaa tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac
    28081 ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt
    28141 tgatctctgg agaaaaactg atcctatata ctggtggtga taagctgatg cctgacgtaa
    28201 caggatggtc aaaagcagat atcatgaagc tggggaaaat cctcggtatt gaagttagtt
    28261 ttgatggaga tggttattgt gtcaaacaag aacttgctcc atatgaaaaa atcactgaag
    28321 acaaacttag ttttacttta gaagaataag aagagaaata ggagagaaaa gagcatggaa
    28381 tggacacaag cattgattcc cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg
    28441 ctatttattg gttattttca aatgaaaaag caaggtcagg cgattcgtga ggagggacca
    28501 aagtggcaca atagcaaagc cggaactcct acaatgggcg gcttagtgtt tctgatcggc
    28561 tctattttga caggaatttg ggttggagct tggcaaaaac agctaactcc tactcttttc
    28621 atcttgcttt ttgtattagc gttatacgga gtaatcggct ttcttgatga ttttataaaa
    28681 atattcaaga aaagaaacat gggactgaac tcgaagcaga aattgattgg acagattcta
    28741 gggggaatca tcttttatct tgtttaccgt tctgaaggtt atccaggtgt attgaatttc
    28801 ttcggactag atctaccttt aggaatcatt tatggtattt tcgctctctt ctggcttgtt
    28861 ggattttcaa atgcagtaaa tctgacagat ggcatcgatg gattagttgc tggcttagga
    28921 agtatctctt ttgcagctta cggtctaatt gcttggcatc agcatcagta tgatgtatta
    28981 atcatttgct taagtgtttt aggaggttta ctgggtttct tcgtctacaa cagaaaacct
    29041 gccaagattt ttatgggaga tgtcggttca ttagcgttgg gcggactttt agcggcaatt
    29101 tctataatgc taaaccaaga atggacattg cttttgattg gtttgatcta tgttatggaa
    29161 acagcaagtg tgatgcttca agttacttcc tttaagctta caggaaaacg aatttttaaa
    29221 atgtctccaa ttcatcatca ctttgaaatg tgcggatggt cggagtggaa gattgataca
    29281 gttttttggt tgatcagcat tgtgacttcg ttgatcacat tatggatcgt ttggtaggag
    29341 atgacgggaa atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg tcttaggact
    29401 cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt
    29461 taatgatgga aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt ctttagggat
    29521 aaaagtaata acaggtagtc atccaatcga attgttggat gaagaatttt cattgatggt
    29581 caaaaatcca gggattccct acagtcatcc tttagtagca aaagcccaag aaatgggtat
    29641 tccggtaatt acggaagttg agctagctta cgaagtagcg gaatgcccaa ttattgggat
    29701 tacaggaacg aacggaaaaa caacgacaac aacgatgaca ggactactac tgaatgcagg
    29761 agcagatcaa ggaattgcac gtttagcggg taatattggg tatccagcaa gtggtgtagc
    29821 gcaagaagca aaatcagaag ataaaattgt gatggaattg tctagcttcc aactcatggg
    29881 aatcacagat ttccgccctc atatagcggt gattaccaat atctatgaag cacatattga
    29941 ttatcatggt acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac
    30001 agaaaaagat tatctgatcc ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag
    30061 aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc
    30121 tgacgattac tcaatatact ataaaaaaga aaaaataatg gaaatatctg aacttggcgt
    30181 accaggtaag cataatgtag aaaatgcact agctgctatt tctgtagcaa aattgtatgg
    30241 tatatcaaat gaagctatac gagaaacgtt gcattttttc catggcgtac cacatcgtac
    30301 gcaatatgta ggagaaatcc aaggcagaaa attttataat gattcaaaag caaccaatat
    30361 tttagcgact aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt tattggctgg
    30421 cggattagac agaggaaata catttgatga attaattcct tcattaaaag gaatcaaagc
    30481 aatggtcgta tttggccaaa caaaagaaaa gttgatggat gctggaaaaa aagcaggcat
    30541 cgagacaata gtaacagccg attctgtaga acaagcagtt cctttagcat tagaaaacag
    30601 tacagatggt gatgtagttt tattatcacc agcgaatgcc agctgggatc aatacccaaa
    30661 ttttgaaact agaggaaaca gattcatgga agctgtgaac cgattaaaat agaaaaagta
    30721 gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg ggacatattt
    30781 atccagctct tgcctttgtc aattatgtaa agactcaaga accgaatgct gaattcatgt
    30841 atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga atgcctttcc
    30901 ataccttgga gattcaagga tttaaacgaa aaatttccat gcataacatc aagacggtcc
    30961 gattgtttct taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt caaccagata
    31021 tcgttatcgg tactggagga tatgtttccg gagcggtagt gtacgctgca gccaaaatgg
    31081 gtattccaac agttatccat gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa
    31141 gccgatatgt ggatcggatc gctctttcat ttaaagatgc ggcttccttt tttcctgaaa
    31201 acaaagcagt tcttgtaggt aatcctcgag cacaagaagt agcagatact aaaaaatcag
    31261 atattttgaa aacatttgat ttggatccag aaaagaaaac agtcttgatt tttggcggta
    31321 gtcaaggagc acttaaaatc aatcaagcag tatcagtttt tttaagtgaa tatattcctt
    31381 cttcttacca agttttgtat gcttcaggag atagatatta tgaagagatc ctaaataaaa
    31441 taaatcctga aatacaaaat attagtgtcc agccttatat caaaaatatg gcagaggtca
    31501 tggcttccag tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag
    31561 ctctggggtt accggcagtg ttggttccca gcccatatgt tacgaatgat catcagacta
    31621 aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat gattgcagat aatgaactaa
    31681 caggtgaatc actaagtcag acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc
    31741 aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact
    31801 tagtaaaaca aataatcaaa aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc
    31861 aaaaaaattg tctcatccgt cttctgatga aaacaatttg actccttggc aaaaggctaa
    31921 ccgaaaatat ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag aagacataac
    31981 attagataca cccctttcag attctgaaga agaaaaaacg gttcaagagg aacagcaaga
    32041 agaaagccag tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg
    32101 tggaccatat aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa
    32161 agtactattt cgtcggttag ctttgatcat cactgtattg ggtataccgc tgattttttt
    32221 gatctattat gtttctcctt atagcaaatt acaagcagtt gcagtatcag gcaataagac
    32281 agtcaacagc caagaaatca tctctgatac aaaattgtca ttaggagaaa atgtttgggg
    32341 acagtatttt catcgatcta cttatatcga tcgccttaaa aaagcacagc caagaatcga
    32401 aacagcaaat atacatttca aagggatgaa tgaattcgat ttggatgtaa cagaatataa
    32461 agagattgct ttgatcgcaa aaaacaatca atactaccca gtgattgaaa atggaacagt
    32521 attagatgaa aaagtagcaa atcctacaaa aaatctccct atcttggaag agttcaaaga
    32581 ctctgcaaaa atcaaagaat taacaaagca atacaatcag ttatcatcag aattacaaaa
    32641 agcaatttct gaaatcaagt acacgccaag agcaagcaat aaaaatctga tccaattgaa
    32701 tatgaatgac ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc aaatgaaata
    32761 ttattcgcaa gtagccaaag atatggatga aaaaggtgtg atcgacatgg aagttggtat
    32821 cttctcgcat ccttacggta gcactcaaga aacggaagaa tcgggccaat cggaagaaac
    32881 aactgaaagt gaagaaaatg cggatcaaac aaacgaatca agtgaagagg cagaaacgca
    32941 aaaagcacaa acacctggat cgtctagcac aaacggatca tctgattcaa atgattcaag
    33001 tgaaacaagt caaatagaag ataatccaag tacaactccg tcaagtttaa atgaaaattg
    33061 ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt atggtagaat
    33121 tggaagcagt gaatatatct attaataagt atattttaaa acggacgcta gttgttcgct
    33181 tgtatatgga atagtaagta ggagggaatc ccattcatgg caaaaacagg aatgtatgta
    33241 ggccttgata ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa
    33301 atgaatatta ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt
    33361 gatatcgata aaacggtcca agcgatacaa cgagcagtac gacaagcaga agaaaaagca
    33421 ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga agtagaaaat
    33481 tgccagggaa tgattgcagt taacggagat tcaaaggaaa tcaccgatga agatgtcaga
    33541 aatgtggctt cagctgccct tgttcgttca atacctcctg aacgtcaaat cgtgtcaata
    33601 ttgccacaag attttactgt cgatgggttc gagggaatca aagatcctcg tggaatgatc
    33661 ggcgtaagat tagaaatgta cggtctgttg tttacaggac ctaaaacgat catccataac
    33721 atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat tactccttta
    33781 gcgctgactg aatcaattct ttctgacgga gaaaaagatt ttggtacgat cgtcattgat
    33841 atgggtggcg gccaaacaac aacggctgtt atgcatgata agcaattgaa gtttaccaat
    33901 cttgaccaag aaggtggcga atttgtcact aaagatatct ctattgtatt aaatacgtca
    33961 tttaacaatg cagaagcgtt gaaaataaac tacggggatg catacccaga acggacatca
    34021 ccagatgaag aatttccagt agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa
    34081 cgctacttgt cagaaattat ttcagctcga atggaacaga ttttcaacaa agcaaaagat
    34141 gcattggacc aaatcgaagc attagagttg ccaggtggcg tcgtattgac aggcggagct
    34201 gctagtcttc ctggtgttgt ggacttggca caagaaatct tcggcgtaaa tgtaaaattg
    34261 tatgttccaa atcacatggg gctgagaaac ccggtattta caaatgtaat aagtatcgtg
    34321 gattattcag caaacttgag tgaagtgtat caattggcaa aaattgctgt tacgggtgaa
    34381 acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa caaacacata tgagagctat
    34441 gaagcacctg aagaaacaat ctacgatgag ccggaaccaa aagagtccgg agaaaatgta
    34501 aaaaataaaa tcaagggttt ctttacaaat atttttgatt aattcatcct gggaggaata
    34561 agatatggaa ttctcaattg ataataatat caatgacggc gcagtaatca aagtcatcgg
    34621 agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt
    34681 tgaattcatt acagccaaca cagacgtgca agcattaaaa aattcaaaag ctgaaacagt
    34741 tatccaatta ggccccaaat atactcgtgg tttaggtgct ggttctcagc cagaagttgg
    34801 acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg gtgcagatat
    34861 gattttcatc actgcaggaa tgggtggagg aaccggtaca ggtgctgcac caatcgttgc
    34921 aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc cattcacttt
    34981 tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc gctcgtttga aagaaaatgt
    35041 ggatacatta ttaattatct caaataaccg tttattagaa gttgtagata aaaagacacc
    35101 gatgcttgaa gctttccgtg aagcagataa tgttttacga caaggtgtac aaggtatctc
    35161 tgatttgatc acagcgccag gttacgttaa ccttgacttt gctgacgtga aaacagtgat
    35221 ggaaaaccaa ggtacagcat tgatggggat cggtgtggct agtggagaag atcgcgtagt
    35281 agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa acatctattg atggagctga
    35341 acaagtactt cttaacatca caggcggttt ggatatgaca ttgttcgaag cacaagatgc
    35401 ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat atcattctag gtacatctat
    35461 caatgaagaa atgggcgatg aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc
    35521 aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca caaatgcaat cacctgctca
    35581 aaagactgta ttagatatgg atcaagctaa accaacttct tcagaagaag aaaacagttt
    35641 tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct agagttgatg attccaattt
    35701 tgacaatatc gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa aatctcatgg
    35761 cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga gaggaagagc
    35821 acatgattgc tgacaacttg cagaaaatca agcaagaaat tcaggattcg tgtgctcttg
    35881 ttcatcgtga tccgaaagaa gttacattag tagctgtaac aaaatctgtt gaatccgctc
    35941 aagctgaaga attggttaga gctggcgtta tgaattgtgc agaaaaccga gtagataaat
    36001 tattagaaaa gaaacaagat ttatttgatt ttcctgaaat aaagtggcat ttaattggta
    36061 atttacagcg cagaaaggta aaattgattg taaatgaaat tgattacttt catgcgttgg
    36121 acagtttaag attagcagaa gaaatccaga agcggttaga gaaagaatta tcctgtttta
    36181 ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat ttcaccagat gagcttttac
    36241 cgtttgtgga atctttagct gcctgcacaa aaatcaaggt tgttgggttg atgactatgg
    36301 cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc
    36361 gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta agtatgggga
    36421 tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac atttatccgt gtaggaacag
    36481 cgctattcaa agacgaataa aggaggaaca aaaatgccgc ttttaaacag aggagcaatt
    36541 gctaattttt tcggattagc tgatgaggaa gagtacgaat accaagatta tcctgaaaca
    36601 acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac agaatcaatc aactcattct
    36661 gtatctgctg cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat
    36721 gaaagtattc agccggctga aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa
    36781 actgtagcga aagaaagaac agcttcaaga acctctacac aacctaaagc aaatcttgct
    36841 ggaaaaatca ctgtaatgga accaagagct tattctgaag ctatggcgat tgctaaacga
    36901 atcattgctg gagaagcaac cttagtgaat tttcatttag tagaagaaaa acaagcgaga
    36961 agaatcgttg attttctaac aggaacagtt tatgcactag atggagatat caaacgtgtt
    37021 ggagatgaga tttttctttg tacaccgact gggatggaga ttgacagttc aaccgctcaa
    37081 tcttttgcag atgcgaattt gtttgatctg taagaggagg aactacgatt tatactattg
    37141 cactattact tcatttactt caacgggcag cctatttcta tagcattatc ttgatggtat
    37201 atgcattgct ctcttggttt ccaggcgggt atcaatcagg gctaggacga ttattagcta
    37261 agatctgcga accgtatatc agtttgttcg atcgtctgcc tttgaggatt ggacccttgg
    37321 atttcacaat tttaggggca atcctgatct tgaacttagc cgtacaaggt ttagcacaga
    37381 tcgttatatg gataactgtc catttagttt attgattgaa gcaacaaaat ctttattctc
    37441 agaatagata ggaggggaaa attttggatg caaatgtata ccagcacttt cgggcagatg
    37501 aacgtacatt tatcgattcg gtaggcgatt ggattgaaca ggttcaagga caatatgcac
    37561 cctatctgac cgaatttctt gacccaagac aatcctatat tttagaaaca ctgattcgac
    37621 aaagcagtga tttgcgtttc atgttttatg gcggatatga acaggcagaa agaaaaagat
    37681 gtttgatttt ccccgattat tacgaacctg tagaggaaga ttttgaaaca gcattgtatg
    37741 aaatccatta tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt ggaactttag
    37801 tgggaacggg gatcaaacgc tcttactttg gcgatatcat ctcggacggt gatcgctggc
    37861 aagtatttat tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca aagattggaa
    37921 aggttgctgt acgcttggaa ccaatcaaat acacagaaat gattatgccg aaagatagct
    37981 ggacacaaga aagaggaaca gttagttcca tgcgactaga cagtgtgatc tctgagattt
    38041 ataatatttc cagacagcgg tccaagcagc tgatcgaagc tggtaaagta aaagtgaact
    38101 ggacagaaaa tacaagacct gattttgtac ttgaactatt agatattatt tcagtcagag
    38161 gttatggcag agtccagatc caagaacttg aggggaagac gaaaaaagaa aagtacaggg
    38221 tactgttcgg tgtattacgt aaataaaata aaaaggtggt ttactcatgg ctttaactcc
    38281 attagatatc caaaataaga cattcccgac aaaaatgcgg ggatataatc aagacgaagt
    38341 agatgatttc ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga
    38401 attagaaaaa gcagtcaaac attctgaaga aaaacttgag tacttcaatg aattaaaaga
    38461 tgctttgaac caatcaatca tcgtagctca agatacggct gataaagtaa aaacaagtgc
    38521 aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac aaagcagatg agttagttgc
    38581 gaacgctgaa aaacgtgcac atcaattaac aactgatgca gaagaaaaag caagaaaaat
    38641 cttgacagat gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag atcttaagaa
    38701 aaaaacacga gtattccatc aacgtatcag cttgatgctt gagtctcaat tagaacaagt
    38761 gaaaagtccg gaatgggatg aaattttaca accattctcc agctacgtga ctgacagcca
    38821 tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa
    38881 ctcagacaca ggagtcatag aagcacctat gacggcattt gatacacagg ctattgatat
    38941 ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga aaagcacttt gttattctta
    39001 aagcgagccg atgagagtgg aagttcggta agacactgca tggtgaaaga tcctctactt
    39061 gagacctgct tttccgaaat tagagtaaga agcagcggct gacctcgtta ttggtcacag
    39121 agagtttgca ctagatagat gcaactaaat ttgggtggta ccacggcatg ttcgtccctt
    39181 gcggctgaac atgctttttt gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa
    39241 aatgaaagaa accttgcaat taggaaaaac aaaattccct atgcgtggta atttgccaaa
    39301 ccgagaaggc gagtggcaaa aagaatggga agaaaatcga atctaccaaa aaagacaaga
    39361 gttgaacgaa ggaaaaccta gctttgttct tcatgatgga cctccatatg ccaatggtaa
    39421 tatccattta ggacattcat tgaataaaat cagtaaagac attatcattc gttcaaaatc
    39481 aatgtctggc tatcgtgcac catatgttcc aggatgggat acacatggat tgccgattga
    39541 acaagtattg gctaataaag gcgtgaaacg taaagaaatg tccatggcag aatacttgga
    39601 aaaatgtcgt gaatatgcac tttcccaagt agataagcaa cgagcagatt tcaaacgcct
    39661 aggagttgca ggagattggg aacatcctta tgttacatta gatccttctt atgaagctgc
    39721 acaagtacgc gtatttggta aaatggctga aaaaggctat atttacaaag gattgaaacc
    39781 aatttactgg tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg aatataaaga
    39841 tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa gacggcaaag atattcttga
    39901 tgaagataca gcttttgtca tttggacgac aacaccatgg acattgccag caaaccaagg
    39961 tattgctgta aatccaaaat ttacgtatgt attggtggaa gccgatggcc gtaagtttgt
    40021 cgtagcgaaa gatttgctag aaacagtcca atcagaaatc ggttgggaaa atgtaacagt
    40081 tctaaaagaa tttgctggac aagaaatgga atatatgact gcaagtcatc cattctatga
    40141 acgtgaatca cttgtgattt taggcgatca tgttacttta gacgctggta ctggattagt
    40201 ccatactgct ccaggacatg gggaagatga ctatatcgct ggtagcaaat ataaactatc
    40261 tgtagtgtca cctgtagact caaaaggatt gtttacagaa gaagcacctg gatttgaagg
    40321 tatcttttat gacaaagcaa atcctatgat cactgatcta ttaaaagaaa aaggagcttt
    40381 gctgaagcta gatttcttta ctcatagtta tcctcacgat tggcggacga aaaagcctgt
    40441 gatctatcgg gcaactccac aatggtttgc atctatcgat aaattccgtc agaatatttt
    40501 ggatgaagta gaaaaagtag agtgggttat cccatggggt aaaacacgtt tgtataatat
    40561 gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag taccacttcc
    40621 gattttctat gctgaaaatg gagaagcaat catcacacca gaaacaatcg aacatgtggc
    40681 aaaattattt gctgaacatg gatcaattat ctggtttgaa aaagaagcga aagaacttct
    40741 tcctgaagga tttacccacc caggttctcc taatggcgaa tttacaaaag aaaaagatat
    40801 catggatgtc tggtttgatt ctggttcttc tcatgaagct gttttacgtc agcgaccaga
    40861 attatcattc ccagcagata tgtatttaga aggatcagac caatatcgtg gctggttcaa
    40921 ctcaagtatc acgactagtg tggctatcaa tggtgtagca ccatacaaat cagttatttc
    40981 tcaaggattt gctttagatg gtgaaggccg taagatgagt aaatctttag gaaacatcat
    41041 tgcaccagaa aaagtgatca aacaatttgg tgcagatatc cttcgcttat gggtatctag
    41101 tgtagattat gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt
    41161 atatcgtaaa atcagaaata cgatgcgttt cttattagcg aatacagaag atttcgatcc
    41221 taaaaaagat actgtttcgt acaatgattt acgttctgta gacaaatata tgaccgttcg
    41281 attgaaccaa gtaatcaaag aaatccgaga agaagggtac gacaaatata acttcatgca
    41341 tatttaccgt acagttatga acttcttgac ggttgatcta tcgtcattct acctggattt
    41401 tgctaaagat gtagtttata ttgaagcaga agatgattat caacgtcgtt gcatgcaaac
    41461 tgttttctat caaacagctg tagctttgac aaaattattg actcctatca ttccgcatac
    41521 agcagaagaa atctggtcat tcctgaaaga agaagaggag tacgttcagt taagcgagtt
    41581 tcctggttat gaagagtttg caaaccaaga agaattgatg gatacctgga ctgctttcat
    41641 ggacttccgt gataatgtct taaaagcatt ggaagaagca agaaacagca aattgattgg
    41701 taaatctttg gaagcaaaac taactgttta tccaaaacaa caagttcgtg aaatgttaaa
    41761 agctttggat gcagatattg cacaattatt gatcgtttca ccagactact ttgaagttat
    41821 ggcagcagat gaacaagtgc cagataacgc tatggtattt gatgatgttg ctattacagt
    41881 ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt
    41941 agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc
    42001 tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc
    42061 tttgtcacac ccccttttgt tatcaatcgt ttaattttcc acgttcacga taccagttat
    42121 ctggttgcca gatccattcg aatccgtctt tttcaagtag ttcgaaagct gctttaggtc
    42181 ccattgatcc agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa
    42241 tgacatccac gatatgccaa gattgagcga cttcttccca atgagagaag ttcgttccat
    42301 caccattcaa cgcatctaat agtaattttt cgtaagcttc tgggctattt tcaaccattt
    42361 ctgcactatt tctaaattcc aatttgattg gttccgtttc aaatccttgc cctacttctt
    42421 tcccattcaa ggataaagaa aatccttcag ttggctgaat atagatcgtt agaacattag
    42481 gtggaagtgt cgtatcgtca catggttcat cgacagatgt tttgaataca ttaacaggaa
    42541 cttgtttgaa tacgatgttg atccgagtcc ctttttcagt caaacgtttg cctgtacgta
    42601 catagaaagg aacacctgac caacggaagt tatcgatagt gaatttgcct gcgacgaatg
    42661 tttcagtctc tgattgagca tccactttgt cttcttcgcg atagcccttg aattgtacac
    42721 catccaattc accttgagca tattgcccgc gaacgaagtt ttctaacact tcttcttcgt
    42781 tataaatgcg gatagctttc aatgctttga tcttttctgt acggatttct ttttcggaaa
    42841 atgctactgg cggttccatt gcaagtaatg ctacaacctg taaaatatga ttttgcacca
    42901 tgtctttaag tgcgccacta tgatcgtagt agcccccgcg atcttctaca ccgaggtttt
    42961 cgccaaaagt aatttggata ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat
    43021 tattggcaaa acggatagct gagatgtttt ggatcatttc ttttcctaaa taatgatcaa
    43081 tcctgaaaat atcttgttct gggaatactt ctctgatttg ctggttcaat tcaaaagcag
    43141 actggtaatc agaaccgaac ggtttttcta tgatcaaacg attgaagcca ttttttgtca
    43201 taatattttg tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt gccatagcca
    43261 aataatagac acggttaccg tttaattgat agtgtccatc taatcggtca gacaattctt
    43321 ttaatgtctg ataatgcgtt gaatcattta catcatggga ttgatagtaa aaatggcttg
    43381 caaaggtagt tgcttcttca tctgaaggat tcaatgcacg gattgtttct ttgacaactt
    43441 cacggtagtg ctcatcgctc cagggacggc gagcggttcc gattacagca aaatgctcgc
    43501 ctaaatttcc ttttctgtaa aggcggaaca aagacgggta caatttacgc tgggctaggt
    43561 cacctgtacc gccgaatatc gtaaacaata catttttttc attagacatg tggatacact
    43621 cctttatatt ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg ggattttctt
    43681 tctatcattt tggatagtga tcggtccttc ataagctgcg atgtcaacga tctcatattc
    43741 ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac aattcttttt catcaagcac
    43801 tcgtgcgata cgcacttttg taccgacagg ataggactcc aatgtttgtc ggcgttcttc
    43861 atggacaact tcctcttttt taggaatcga tcctccatga ggacaatgtt caggatgatt
    43921 caaatagttt tctaaatgat ctgcaagatg ttccgaagtc acatgttcca atacttcagc
    43981 atcatcatgg acttcattcc agctatagtc taaatgttca actaaaaaaa cttcccataa
    44041 gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg cttgcagtca attgtacacc
    44101 ttgataaggg gaatgttcta caagctcttc tttcacaagt ttagaaatca tttcactgac
    44161 agatgcagct gagacatcta gactagatac gatttgttta ttgttgactt tcgttgtatc
    44221 tccacctagt tctaaaatga ttttcaaata atcttctcga tttggtgtca tattcgatca
    44281 tcctttcatg gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt aattgactat
    44341 ttttctaaat aaaaaacaaa ggatttttag acgtttttac aacaaaaaac gaaagatgat
    44401 agagaagact cattctttcg tttataaagg atatatatat acattgggag ggatctattt
    44461 ttcagataac aacttatatg aagtgtctgc agattcgtca taaagagttt gttcgggata
    44521 agaaaagata agataaaaac catactgatc acgtacgact aaacgatatt catctatttc
    44581 taccacagat gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg
    44641 taattgttgt ttttgctggc tatcactaaa gaaccatgta ccttgatact ttgagagtcc
    44701 ttctgacttt tccgcttgtt ttttcttttt caagtaaata aaacttgaga gaacggtaat
    44761 gagtgaacca agaaaataga tcattttttt tatcaccaaa tcactccttt atgagaaaat
    44821 aatcgcatag acatattctt tcttctttat ttcattgtac cgaataaaag ctaaaatttc
    44881 tctaagactt tcgactggtt tttttactgt ttttctacaa attatgatag gatacatagt
    44941 aggaagaaag gtgacataat ttgaagtatc agcaagtgga aatagctttt tttattgacc
    45001 gcccgaatcg gtttatagca cattgtatga aaaaagacgg agaaataatt attgcgcatg
    45061 tcaaaaatac aggtagagga aaagaggtcc tgataccagg cgctgaagtg gctattgtat
    45121 ttgctcctgg acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg
    45181 actggtttaa tatcgatagt caattgccta atcggctggc aattgatggt attttagacg
    45241 gcacgatcct tttaccagga ttaactggtc catttacggt atacaagcgt gaagtaactt
    45301 atcttcactc caaatttgat atttatttag agacggtggt cggacaaaaa gcatttgttg
    45361 aagtcaaagg tatgacactt gaaaataaag cgattggtgc ttttcctgat gcgcccacac
    45421 tcagaggatt gaaacatata ggagaattaa cgtatgccgc acaagatggt tatgccgctt
    45481 atgtcttgtt tattgctcag ttcgaacatc ttcatctggc aacgatccat gaagaaatgc
    45541 aaccagcact agctgatatg gttcgacatg cgcagcaaag cggagttcaa atcctcgcct
    45601 ataattgtca agtatcggca gatgaggtaa ctgtcaagca ggccattcca tttgatgtag
    45661 atatcccttt tgaagatcct atacaataga aagaaggtaa aataatgatc aacttaggta
    45721 ttatcggaac aaattggatt actcatcaat ttgtccaagc agctttaagc acaaaaaaat
    45781 atgaattgac agcggtatat tctagacatt tggaaaaagc acaggaattt ggttctcatt
    45841 atgaagggga tatcgaattc gcaacagatt tgaagacttt tttcaatatt actcatatgg
    45901 acaccattta tattgcttct ccgaatagtc ttcactttag tcaagctaag caagcaattt
    45961 tggcaggaaa aaatgtgata gttgaaaagc cagctttttc gacgccaaaa gaaatggatg
    46021 aaatcatcca tttggcaaat gaacacaatg tgtatttttt tgaagcagca agaaatatcc
    46081 atgaatcagg ttttaaaaag attgccgatt acttaccatt aaaagacgag ataatcggtg
    46141 caaatttcag ctatatgaaa tattcttcgc gctatgacca agtattagat ggagaagaac
    46201 cgaatatctt ctcgactcat ttctcaggtg gtgcattgat ggatttaggt gtttaccttg
    46261 tatatgcagc tgttgcttgg tttggaatgc caaaagaagt ccattatttc ccaagaaaga
    46321 tttctactgg tgtagatggt cttggatggg ggatcttacg ttatgagaat tttgatgtat
    46381 cgatccagcc aggtaaaatc ggtgattctt atttaccgtc agagatttat tttgactcag
    46441 gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc
    46501 ataaagaaat cattgatttg gcggtaacag ctgcagaaaa tccaatggca gatgaagcag
    46561 aggattttgc caatgtcatg gctcatccga aagatccagc ttggggattg ttgtatgaag
    46621 agtggatcga gctagctaga aatgtgaacc aagtgattta tgatttaaga aaaaatggcg
    46681 gcatcaaatt cgatgcagat aatgaggaag atttttaatg aacgaatttg tacaagtatt
    46741 tcccgaaaat tggcagaaaa aatatgagga taaagggttt atctcacctt ctcttattca
    46801 acgagcagtt tttcagccac taagtaataa acaaagtata gtagcggttt ctcctacagg
    46861 ttcaggtaaa acactggctt atctttggcc tctactacta aacgtggaac caggtgaagc
    46921 aagtgcgttg gtcatttttg cttcttctca agaactagct attcaagtag cagatgttgc
    46981 acgtgaatgg ggaaaagaca aagagttgaa agtccaatct ttggtgggcg gagccaacgt
    47041 caaacgtcag attgagggtt tgaaggaaaa accagaaatc ttgattggta caccaggaag
    47101 aatattggaa ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa caatggtatt
    47161 tgatgaagca gaccagctat ttgacgctgg gaacagccaa atcattgatc aaattcttca
    47221 tcaagctcca acagagtatc agctagcttt tttttcagcg acagcagatc gttcccttga
    47281 aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag cagaggacga
    47341 ttctcgaaaa ggactgagac attacttttt acgtgtaccg atacgaaaga aagatgacta
    47401 cttgagacgt ctcacgcata tagaagattt tcatggtctg attttcttta atcaactgac
    47461 tgaattaggc gtaatggaag aaaaattgct gtatagaggt gtaccggtag gaagtctggc
    47521 ctctgatcag aacaagctac tccgaaaagc agcgctagaa caattcaaaa agaaaatggt
    47581 cagagcactt ttgaccacag atgtggcggc tagaggttta gatattacag gtcttcctta
    47641 tgtagtgaat gcagaagttc cgttaagtga agaagcttat ttgcatcgtt cgggaagaac
    47701 tggtcgaatg ggtaatgaag gaatagtaat tacactcgta caagataata atctaagaga
    47761 tttaaaacgg cttttacgtc catcaaatat aacacttgaa gagatttttt tatacggagg
    47821 taagctgcaa actgagcagc caaccaaaga agatgcagct cctgcagtaa ataaaaagta
    47881 tgcgaaaact ttttctggaa aggagcagaa cccagaatat aacaaagaaa aaataagaaa
    47941 aaaagttaaa aataaatcaa aaaaagagag aaataaagga gcaaggcgca aataagcgcc
    48001 tttttatgct atgattatga aaacgggaac aaaattggtt gccagaggag gggttatgtg
    48061 aaagaacgtc aagaagagat cttgcagcta ttgacagaaa accaacaagg attgacagca
    48121 agtgatgtag ctgagcgctt aactatcgat cgaagcaatg caagccgata tttaagtgaa
    48181 ctatacaagg cccatcatat tgtaaaaacc gctggccgac cagtcgttta ctctttgcca
    48241 acggaaaagt caaaatctga tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa
    48301 acactcgttg gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt
    48361 ctttatccac cgcgggggtt gcatacgatt atttttggag aaactggtac agggaagtcg
    48421 atgtttgctg aatgtatgta tcattttgca atcgattcag agatgctttc tgcagatgcg
    48481 ccatttgttt ctttcaattg tgcggattat gctcaaaatc cgcaactgct ctttggacac
    48541 atctttggga tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc cggtctaata
    48601 gccaaagcag acggcggtat attgttttta gatgagatcc accgtttacc gccggaaggg
    48661 caggaaatgc tgtttacttt cattgataaa ggtatttacc gtccgcttgg agaaagtgca
    48721 caagtacatg aagcatctgt tcagatcatt ggggcgacga ctgaatcttc agaaagtttc
    48781 ttatctacgt tcaatcgacg gattcctatg gcaatcacct tgccgaatct tgcttcacgt
    48841 tcactggatg agcgttatga aatcatctct ctttttatca aacaagaagc gaatcgtttg
    48901 aatcaacgta tcgatatcga aaaagaagcg attcttgcct tcatgcttta tgatgcggaa
    48961 ggaaatatcg gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc ttttttacat
    49021 taccgtactc atcatgagaa aaagctaatc attcgaaaag aagaactttc ccttcaagta
    49081 caaaaaggat tactgaaaat caaggaaata ccggaacgtc tcgaccgatt catggacagc
    49141 aaaagtcagt atttgacatt tgaaccagga tttgctgatg ttgtctggtc acaagatcca
    49201 gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag tcacttctct ttccgaaacc
    49261 ggtgtggaga acattgatct agagaccctc atttcaaaag atgtggatgc ttatttccag
    49321 acatatgtca aagaattgac taaaagttcg attcccaaag atcttttgcc agatgaaatc
    49381 tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg tatttataat
    49441 gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta cattggatcg tgtgagagaa
    49501 ggtcatatga tcgtccaccc agatttgaat aacgtacgga aaaattttaa gagggaattc
    49561 caggtagcaa tcgatctttc aacgatcatt gaagaagaag aacaagttga gattcctttt
    49621 gatgaaatcg gctttattag tatgtttctt tctatcaatc taggagaaag tgagcctgtt
    49681 aaagaaaaca aagtagatgt tctcatattg atgcacggca gacagactgc ttcaagcatg
    49741 ttggagatgg cacaagaact attaggaaca aaagtaggga aagcttttaa tatgccactg
    49801 gttatggaag ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag
    49861 catttgacga atggcctact cttgttaaca gatatgggat cattaaacac cttcagtgat
    49921 ttgatttatg aagagactgg cattcggacg aaagctatca cgatgacaag taccatgatt
    49981 gtgatagaag cgatacggat ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat
    50041 atccagcttt cctttgaatc gatcgtacgt gatcagttca aagcggaaaa gtctgaaact
    50101 ttacagcgta aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt agctgccaaa
    50161 ttatatcagc gtattatgcc agtgattaat caaaataagg tagaactgat ccagatgcag
    50221 tttttagaac gtgaatcatt caaaaaacac atcgatgcct tattggaaga atatgagatc
    50281 aaagcaattg cgggaacagt tgatgttgat tatcaaaata tcccattttt ttcagcctat
    50341 gacgtttttg atgatgaaaa actaaatatt ttaaagcgta tagctgatga tgagatcccg
    50401 acagctaaaa ttgtcgattc acttgctgga tcattaagcg atgtcttttc tattgatgaa
    50461 ctgatccgtt cattgcaaaa gacagttcag cagatccaga ccgatttgtc gattatcgtg
    50521 gaacctggag tagatactgg aatcgttatc catttagcct tcatgttaga cggattgatc
    50581 aaacaagaaa gccatcggca atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg
    50641 gaaatggata tcgttcgggc aaacttgatg gcactagaac gaaaatatca ggtaaaaatt
    50701 ccagaagatg atatcgcatt tcttacacaa atgtttcttg aaaataaaat aaaaaaatga
    50761 acacactaat catactgttt acacactaaa catctgtgtg cgcactataa aaaagagcta
    50821 aaaagcatga aaacgctatt tcagctcttt tttttattgg catgcttctt gcgttatata
    50881 aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg
    50941 ttcagcaggg atgagtacga gtttattggt cacaaaaatg caaaaagcag cagaagaaaa
    51001 agggatggaa gccgatattt ttgcggtctc tgcctcagat gcagataata atttagaatc
    51061 taagaatgtc gatgtattat tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga
    51121 acaaaaactt gcgcctaaag gtattccatt agatgtcatc aatatgcaag actatggcat
    51181 gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc
    51241 agttgggggt ataacaacgt ggaagatcaa caaaatctag aagccatcat ggggttaatc
    51301 atgtttggcg gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc taaaatcggt
    51361 gattttgagt tagcggatag gaaaatcgct gatgcagaag aatcacttat acaagcgcat
    51421 catgcacaaa cagacatgtt gacacaagaa gcacaaggaa atcatatgca agtgacatta
    51481 ttgactgttc acagtcaaga tcacttaatg acatctattg cgtttactga tttagcaaaa
    51541 gaaatcatcg aactttatcg tcgcgtatac agcgaataaa tacaacttct tttttgaacg
    51601 gaagaagaaa aatttttgaa cggatttttt attttgttat aaggagaact ctctttatat
    51661 aaaaaactta taggaggaag taaaaaatga acggactatc aagttggatg gagaaataca
    51721 tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt agttgctttg cgtgatgctt
    51781 ttatcggcac actgcccgca acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta
    51841 ttcgtgattt accgcaacag tttaacagta cctatgctga cagtttagca gcagacaaag
    51901 gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac
    51961 tagcaatcgc cggattgatc tttgcctttt cttgggggta taatcttgcc cgagcttatg
    52021 gagtaaatga tctagctggg gggatcgttg gtttagctac cttgattcaa ggtatttcct
    52081 ttggtccagc attatcagca gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa
    52141 tcaatagttc tgagattggt gcaacgattg cagatggcac actgtcagtt ggattatggg
    52201 gttggctaaa actagatcat ttgaatggaa acgcttattt tacagttatg atcatgggag
    52261 ctatttcagt cattattttt tgtaaattga tgcttgccaa cattacgatc aaaatgcctg
    52321 attcagtacc accagcagta tctaaagcat ttgcagccat tttaccagcg acgatcgctt
    52381 tatacgtcat tgctattatc aattatatcg taggtcgtgt agctgatggg caactgatca
    52441 ttgatttggt gcaaaaatat attgcagagc cattcctagg attatctcaa gggttgggag
    52501 cagtattgat cgtcacagta tttgttcaag tattctggtt ctttggtatt catggaccaa
    52561 atgtattggc accagtgcta gagggaatct ggggacaagc acaactaatc aatattgata
    52621 ttttccaaaa aggttataac ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag
    52681 atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg tttggcggat
    52741 caggcggaac gatcgtctta ctgatagcta tcttgatttt ctctaaacgt gcagattatc
    52801 taacagtagc taaattatcc attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt
    52861 ttggtctgcc aatcgtattg aatgcaatca tgtttatccc attcttgatt gctccagtag
    52921 tagcaacagc aattggatgg acagctactt atcttggcct agtcgctccg gtatcgcagc
    52981 aagtcacatg ggtagtaccg ccgttcttat tgtcattctt agcaacagga gcggattggc
    53041 gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg
    53101 tcatctctgc aaacaaaatg gaccctgccg caggcgaaca acaataagat gaacgtgagg
    53161 agcgggacgc tttaagtttc gttcctcata atgaattttc gaggaggaac tgaaatgatt
    53221 acaattgcca gtgcaatgca acaagatgaa atcaaagaat tgttagaagg atacaatgaa
    53281 gaaggaatga cttttacatt taaagaaaaa caaggaatca agctgttctt tgaaacaagt
    53341 gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg
    53401 ggaacagtcc tttattttca atcaacagct gtttagaaaa tggtttaaaa agataggatg
    53461 gttgagatga taaaaaacgg tttattttta tgtagtattg gttctttgat cgtgttgtat
    53521 agtttgaatc cacattcgca aaggtatgat ttgtatagta tggtcacagg tctttttcta
    53581 acaggattag gaatctattt cttcatgaag gggaaaaaag agaagaagca aaggagaaaa
    53641 ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt acgataaagt
    53701 tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa ctagaaaaca
    53761 gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc gcataaaaat
    53821 cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga agaatgaatt
    53881 cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc attacacaga
    53941 aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag gaacaatctt
    54001 gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag caacgtatta
    54061 aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag aaggaatttt
    54121 tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg aaggggcagc
    54181 aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat gtttgattta
    54241 gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt tcgtacactt
    54301 gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac aagtcaagtc
    54361 atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc cggcattggc
    54421 tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat catggaaaag
    54481 atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc attctcctat
    54541 cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta tcaactaaac
    54601 agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat ggattttgaa
    54661 gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca acgagctggt
    54721 ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa ttaccagttt
    54781 gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca gcttgcaacg
    54841 tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg cagccttgtt
    54901 cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg cggctacggc
    54961 ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat cacaccttat
    55021 atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt taaaaagaga
    55081 caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa tgaaggaatc
    55141 gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca aacaaaactc
    55201 ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta tcgtaaagtg
    55261 gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga aacgttaagc
    55321 tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa aacgggtgat
    55381 gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga aaatgttaaa
    55441 gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta gggttttctt
    55501 ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta gatgaatcta
    55561 agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac ctccctacat
    55621 tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt tttgcctaac
    55681 ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa gctacaattg
    55741 atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct aagcgatagg
    55801 tcgctggttc aattccagtc aggaacgtac aaataacccc tgtatccttt gcggtacagg
    55861 gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
    55921 tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat gtcttagtcc
    55981 atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa aacttactag
    56041 gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat ttcgattggc
    56101 ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt ataacttgaa
    56161 gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt ttagtaatag
    56221 ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa acggtggggt
    56281 tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta tgctttatat
    56341 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
    56401 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
    56461 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
    56521 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
    56581 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
    56641 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
    56701 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
    56761 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
    56821 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
    56881 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
    56941 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
    57001 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
    57061 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
    57121 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
    57181 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
    57241 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
    57301 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
    57361 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
    57421 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
    57481 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
    57541 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
    57601 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
    57661 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
    57721 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
    57781 tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa
    57841 ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg
    57901 cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca
    57961 aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac
    58021 aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata
    58081 cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga
    58141 agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct
    58201 cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag
    58261 agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga
    58321 tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc caagacagta
    58381 tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc
    58441 cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag
    58501 cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata
    58561 cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt
    58621 tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta
    58681 acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc
    58741 gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc
    58801 aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca
    58861 tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct
    58921 acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca
    58981 atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc
    59041 atgatgtgcc tcctcgttta taattattta acaattatga attataccaa aacatcattg
    59101 aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt tggctaaatg tatgccgcta
    59161 tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca
    59221 cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg
    59281 cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat
    59341 atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact
    59401 tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac
    59461 ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt
    59521 tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag
    59581 aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt
    59641 catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt
    59701 catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga
    59761 aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga
    59821 acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt
    59881 tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca
    59941 caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa
    60001 agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa
    60061 gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt
    60121 gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca
    60181 agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg
    60241 tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt
    60301 agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc
    60361 gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat
    60421 tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat
    60481 tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac
    60541 tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat
    60601 tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt
    60661 cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat
    60721 cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc
    60781 tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca
    60841 tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt
    60901 ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttata
    60961 ataattatga ctcttaatta aagtaggtgc ccatatgctt gtttctttta gtatggttat
    61021 aagatttttt ctgacaattt tattatcact gatattaaca cctgtattca aaatcatttc
    61081 agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag ttccaatgcc
    61141 ttcagcgggc ggactgccta tctttatttc atttgttata tcgactttat tcttattccg
    61201 taaaattatc cctcaatcgt atattgtacc gatcttgatt gcttctttag taatcatagt
    61261 cacaggagtg ctcgatgata aatatgagct gacacctaaa caaaaaagta tagggatctt
    61321 gattgcttct cttattattt attttgtagc agatatacgt attgattcct tcacgcttcc
    61381 ttttattgga tatatccaac taggctggct tagttttccc gtaacgattt tttggatctt
    61441 cggtatcacg aatgcggtca atctgattga cggtttggat gggctggcgg cagggatttc
    61501 attgatcggt ttaattacga taggaatcat tggatatttt ttcctccatg cttctacagt
    61561 gtatattcca gttgttattt tctgtcttgt agcaagtatt atcggttttt ttccttacaa
    61621 tttttatccg gcaaagattt atttaggcga tacaggagct ctattcctag ggtttatgat
    61681 ggccgtcctt tcactacaag ggctgaagaa cgtgactttt gtttcatcga tttctttgct
    61741 gatcgtcatg ggagtgccgg taacagacac gttttttgct atcattagga gaaaagcaaa
    61801 ccgtgtttct ttttctacag cagataaaaa acatcttcat caccgactaa tcagtttagg
    61861 ctttactcat aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt tttctttcac
    61921 agctatggtc atgaattata ctggaagaat cggaacgatc gtattgatta ttgccatgtt
    61981 gtttgctgca attttattat ttgaattgat tggtctgatc aatgagaaac atcagtttat
    62041 tttaaaaaca ttgcgatttt tagggaataa agagtaccgt acgcaagtaa aacagaaata
    62101 tttgaagaaa tggaaccgtc atatagatga ataacaaatt aaaccagccg atccttatta
    62161 gtattgtgac ggcaaacagt gataaaattt ttcggacact agataatttt atttcaactg
    62221 taaaagatga aacagcagta aaaatccgaa tttttgacaa caattcactt cccgagtaca
    62281 aaaaaagact aacagagtat aggaaatatc cctacatcga tatctatttt catgatgaaa
    62341 attccggatt tggttatgga cataatcaca atctgatagc tagcgattct cgctatggca
    62401 ttatttgtaa cccagatatt ttagtggatg aagagacagt tacagcattg gtcttgttat
    62461 tgaaagaaca tccggaatgt gcgatgttag cacctaagat tcttaatgaa gatggaacaa
    62521 ctcagcacct catccgtgag cagctggctg tttttgatta cgttttgcgc tttgtgcctt
    62581 tcaaatgggt caaaaaaaag tttgacaaac gactggctgt gtttgaatgc cgaggattgc
    62641 ctactgatgc taatagttat gtacggatga tctcaggtag ttttatggta gctgatattg
    62701 ataaatttaa agagataaag gggtttgatg agcgtttctt catgtacttt gaagacaacg
    62761 acttgtgttt gagttttgaa aaagcaggtt acaagttatt gtacacaccc ttttatgcag
    62821 ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc gtgtttttga
    62881 cttctatgat gaaatttttc aataagtggg ggtggcgttt tttctgatga aaccagaaat
    62941 attagtagtc gttgtgcttt atcagcaact attcagtcaa tctccagctt atggaccatt
    63001 aagcaaagca cttagcgaaa agcaaattca gcttattatt tacgataata gtcctcaaag
    63061 gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat catcatgatc cgcaaaatcc
    63121 tggtttagcg gtggcttata attttgcgtt gtctcaagca gatgaaggaa cacgttgtct
    63181 cgtcactctg gatcaggata cgaggctagt cgacgattat tttgaactat tgcgtaaagt
    63241 aacctttaca gatgaatgtg ttgctgcagt tccgatgatt ttttctggga gccgacaagt
    63301 ttctccccta tattcagatc gttatatcaa tcgtacagct caagcagtag aagttgaagg
    63361 cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg tcaaatttct
    63421 aaaaaagatt ggcgggttta atcctgcttt ttctttagat tttttggatc attggctgtt
    63481 ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa tggaacatga
    63541 tctttcggtt ttagactatg aaaaagtttc aagtaatcga taccgttcta ttttacaagc
    63601 agaaaatcga ttttatcatg aatatgatca aaggcattta tccagtcacc gacgacaatt
    63661 attgttaaga acagctaaac agttcttgac cgtcaaaaac cggcatatct ggcggatgac
    63721 tttgcgctca tatgttaaga attggaaggt gtgaattcgt gcgttcggtt tgtatagcta
    63781 cctttaatgg aggcagatat ttaagagagc agttagatag tattttatct cagctttcag
    63841 cagaagatga agtagtcatt tctgatgatg ggtcagtaga tgatacatgg gatatattaa
    63901 ctgaatacgc agaaaaagat aagcgaatcc agcttttttc ggggcctcgt caaggattga
    63961 tcgctaattt tggttatgcg atccagcaga cgaaaggaga tctaatcttt ttggcagacc
    64021 aagatgatgt ttggttggag acaaaagtga aaaagatcac tgactatttt gagcagaatc
    64081 cggatcagct agtagtagtt tctgatttaa tcattgtgga cagcgagctg caagagatcc
    64141 atgattcgta tttttcgttt cgaaattcca aagaaggatg gcttaataat gtgataagaa
    64201 gtcactatat tggtgctggt atggctttcc ggtcttcttt aaaacccgat attttgccga
    64261 tcccgtcaga agtgccgatg catgatatgt ggattggttt gctgggagga aagaagactg
    64321 ggttcctaag agaaccgtta actctgtacc gaagacatga tttaaacgcg agtgagattg
    64381 aaacacattc taaattacgg caaaaaattt cttggcgtgt tcatctgatt acggcgttga
    64441 tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa atccttaata tttccatgac
    64501 aaatataaca attgatgaga taataacaaa gtgaaatttg aaatcaaaaa ggagagcaga
    64561 tatccatgaa gggaatcatt cttgctgggg gcagcggaac acgtctgtac cctttaacaa
    64621 aggcaacatc gaaacaattg atgccgattt atgacaaacc aatgatttat tatccaatgt
    64681 ctactctgat gttggcggga atcaaagaaa ttctgattat ttcaacgcca caagatacac
    64741 cacgtttcaa ggaattattt ggaaacggaa atgatttagg actgcagatc gaatatgcag
    64801 tacaagaaag tccagatgga ttggcacaag cgtttatcat tggagaagaa tttattggag
    64861 atgatagtgt ctgcttggta ttaggggaca atatttacta tggtggcggt ctgtctaaaa
    64921 tgttgcaacg agcagcttca aaagatacag gcgcgacagt atttggctat catgtcaatg
    64981 atccagaacg ctttggtgta gtagaatttg atgagaatat gcaagctttg tcgattgaag
    65041 aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg cctttatttt tatgacaatg
    65101 aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg tggagaacta gagattacgg
    65161 atgtcaataa agcatatttg gataaaggaa aactatccgt agaacttatg ggacgcggat
    65221 ttgcttggct tgatacagga acacatgaat cattgcttga agcatctacc tttatcgaaa
    65281 caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga agaaattgct taccgtatgg
    65341 gctacatcga taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa aatcaatacg
    65401 gtcaatattt attgaatttg gccgctgaat aaggagtcct tacagatgaa agtaacagat
    65461 acaaaattaa cagatgtcaa aatcgtagaa atggacgtct ttggcgatca tcgcggtttc
    65521 ttcacagaaa gttattcaaa agcaaaattt gcagaacatg gattgaaata tgactttgtt
    65581 caagacaacc attcactatc agctgaagct ggtgtccttc gggggcttca tttccaaaaa
    65641 ggagaagctg cacagacaaa attgattcgg gtagtcactg gagcagtttt agatgtagta
    65701 gtggatatcc gcaaaggtag cccgacttat ggtcaatggg acggatttat cttatctgaa
    65761 cataatcatc gacagttgct tgtacctaaa ggatatgccc atggattcgt tactttgaca
    65821 ccaaatgtga acttccttta taagtgtgat aattactata atgcagaagc agatgggggc
    65881 atcgcatttg atgatccaga tttagcgatt gattggccaa ttgatccatc aagagcgatt
    65941 atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga aaatccattt
    66001 gtttacggag aaatctaaga attaaggaga aattttctta tgaaaaacat cattgttacc
    66061 ggaggtgccg gattcatcgg ctctaatttc gttcattacg tggtaaacaa tcatccagaa
    66121 gtgcatgtca ctgttttgga taaactgact tatgcaggaa acaaagagaa cttggccgga
    66181 cttcctagcg atcgcgtaga attggtcgta ggagatgttg cagatgcaga attagtggac
    66241 cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg cagaatccca taacgataat
    66301 tcattaaaag atcctttccc atttgtacaa acgaatatca ttggtacgta tacactttta
    66361 gaagcttgcc gcaagtatga tgtacgctat catcacgtat ctactgacga ggtttacggc
    66421 gatcttccat tacgtgaaga cctgccagga catggggaag gcgaaggaga aaaattcact
    66481 gcggaaacac catataatcc ttcaagtcca tattcttcta caaaagctgg ttcagatctg
    66541 ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa ctgttcaaac
    66601 aactatggtc cttatcagca tattgaaaaa tttattcctc gccaagtaac gagtatctta
    66661 agcggtattc gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg gatccatacg
    66721 aatgatcatt cttcagcagt gtggctgatt ttgacgaaag gcagaattgg cgaaacctat
    66781 ctgatcggtg cagacggtga agaagataat aagaccgtga tggaattgat cttagaaatg
    66841 atgggccaac caaaagacgc atacgaccat gtaaacgacc gcgcagggca cgatttgcgt
    66901 tatgcaatcg attcgacaaa attgcgagaa gagctaggtt ggcagcctga gtttacgaac
    66961 ttccgtgatg ggttggctga cacgatcaaa tggtacgaag aacatgaaga ctggtggaaa
    67021 aaagaaaaag aggccgttga agcagcatat gccaaaaatg gacaataata cagaggaaat
    67081 ttcactgtat taatggaaaa acacatgtga ggcaggcgct cgcctgtctc ttttttaaaa
    67141 taaaaatcaa ggagcggata accatatgtt tttattaact ggaggaaatg gacaattagg
    67201 tacagaattg cgccatttat tagatgaaaa aaatctgcca tatgtttcaa cagatgcaaa
    67261 agacttggat atcacagatg ctgagaaaac aatggcttat attacagaat taaaacccga
    67321 tgttattttc cattgcgcag cttacacagc agtagataaa gcagaagaag aagcaaaaga
    67381 attagatgag aaaatcaatg tcgatggcac aagaaatgta gcacttgcag caaaagctgt
    67441 gaatgcacaa ttcgtctata tcagtacaga ctatgttttc gatggaaaaa acaaaaatgt
    67501 agaatacgaa gtagacgacc aaacgaatcc actaaatgaa tatggtcgta cgaaattatt
    67561 aggcgaaaaa gcagtagaag agattttaga agactattat atcatacgaa cttcttgggt
    67621 tttcgggatc tatggacaca atttcgtctt cacgatgcaa cgcctagcgg aaacccgcga
    67681 caaactgact gtagtaaatg accagtttgg gcgaccaact tggacacgta ctttggcaga
    67741 atttatggta tatgtcatcg aagaaaaagc accatttggt atttatcatt tgtcgaacga
    67801 aaatagctgt tcttggtatg agttcgcaaa agaaatattg aaagatacag atgtagaagt
    67861 tgcacctgtt acgtcagaag aatttccgca aaaagcaaca cgtccgcagt attcagtcat
    67921 gagcctgaaa aagacagaag cgttaggttt tgtcatacca acttggcaag aagcacttgc
    67981 gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt cctttttatc aaatagaatt
    68041 tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag ccaaaagcga
    68101 atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat cttattggcg
    68161 ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt ggtgtttggg
    68221 ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt ggacagaatc
    68281 gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca caagctgaag
    68341 aggacttttc gttggtgaac gaggattatt cattaaacgg acaaaacatg atcattgcgt
    68401 ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg ggattctttt
    68461 tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc gtcatggtct
    68521 tgcttggatt cgaagtactg atgatattaa cgaagaggaa aaaagctcta tcgctaatcg
    68581 gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa catgtgggcg
    68641 atttgatttt ctttactttg ggactgatga ttgcttttta tcattatttc tacaatcatg
    68701 ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt attgggttta
    68761 ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt ttttattttg
    68821 ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat gtagtgttga
    68881 ttggaggggc agttttattt attggcggag tcatggttca tttctggcta acgtcaaaag
    68941 acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt actggaggaa
    69001 agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct tttgaagata
    69061 tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca actgtattcc
    69121 tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta ctgggacgta
    69181 ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg ccaaaatggc
    69241 tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt acttttagtt
    69301 ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa gatgttattc
    69361 ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc tatgcgatta
    69421 cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt gctgtgttgg
    69481 gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac ctcgcttttg
    69541 tcgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt acaggagcga
    69601 tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat cctggtaaag
    69661 tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg aaatccttca
    69721 atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg aaagaagaat
    69781 acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag gaagatacaa
    69841 agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa gacgcagaaa
    69901 aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac caattatatg
    69961 aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt tttaaagtag
    70021 aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag ctagtcgtac
    70081 agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt gaagataatt
    70141 caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct tttgttgctg
    70201 acgctagtct tttttgtttg gacagcaggg gtaggttttc cagcaacttt gtttatatat
    70261 aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt aaatgtttat
    70321 aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt cattttcttt
    70381 tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt tcctttggtt
    70441 attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg cggtatttat
    70501 ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac agagatccaa
    70561 gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat
    70621 acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt
    70681 tcttttgaac gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc agccttttta
    70741 ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg
    70801 gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ctacacggtt
    70861 acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg
    70921 tttaccgatc gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa
    70981 caagttttac taggaatgaa aacatttatc ttccagcgag gaaaccaatt tttaggaaac
    71041 cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg gattgctgct
    71101 ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag
    71161 atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg
    71221 acttcatacc catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat
    71281 gatgaaccag aagttgcgga ggaattctat gttatggaag aggcatatga cgatatgact
    71341 tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc
    71401 tatcattgat aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc cttttttggt
    71461 ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat
    71521 agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact
    71581 tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat
    71641 ttatgaagga catttatcag aacagaaaat tattgctaca gttttcgatc aatgacttca
    71701 aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta
    71761 ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa
    71821 aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg
    71881 catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat
    71941 tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct
    72001 tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt
    72061 tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga
    72121 cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt
    72181 tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa
    72241 gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa
    72301 gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag
    72361 taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc
    72421 tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca caaaatctta
    72481 taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc
    72541 ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat
    72601 gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg
    72661 agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga
    72721 actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat
    72781 ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc
    72841 tgacatcgga gaccatattt atcaacctgt aaaaacttat tcaagcggga tgttcgttcg
    72901 tctagctttt gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg atgaagcatt
    72961 ggcagtaggc gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa
    73021 ctcgggaaag acgattttat tcgtttctca tgatatcagt tcgattcgtc gtttttgtga
    73081 tcgttcctat tggttgaaaa acggagaagt cgtcgaatat ggcgatacga tggaagtaac
    73141 gacgaattat gaaaactttt taaagaaaaa atcagttaaa acagtggatc ggaataaaaa
    73201 ccaagtagaa cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg aattgttgga
    73261 taaagacaaa aatccggtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac
    73321 ttatgaagta aaagatgata caatcaaaaa tccggtttta ggaattgcgc tacgaacagt
    73381 cgacaatttt tatgtatgcg gacttaatac tctactagat ggtatcaaga ttccttggaa
    73441 aaaaggagag aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata
    73501 ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat ataagacaaa
    73561 gtacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca
    73621 taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac
    73681 aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg
    73741 gtcccggaaa cggccgatta acgaaacatt taatcggagc aaaacaatgt gaagtgagca
    73801 ttgttgagtt agataaggaa ttgttcgatt ttgtcagcga atttgctcaa gatggatttt
    73861 atggtgatat cgaaagtttt gagtgggcaa actattatgc tggacagaca tttgattacg
    73921 tcttgtttgc ggatgtttta gaacatttgg tagatcctgg aaaaacattg aagaaagtca
    73981 gagaattttt gaatgaagaa ggagagatcc tgattacatt ccctaacttg gcgcataatt
    74041 ctgtcatgat cgatttgttc aataaccaat tgccgtgggc ttcttatggt ctcctagatg
    74101 aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcgggat
    74161 tattcattaa tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa
    74221 cttatgaaga attgccggaa gctgtacgct atgatttcaa aatgcgacca tttggtgaag
    74281 tctaccaata ctttttctca ttgatgaaac atccagtagc acaaagttcc attgcagagc
    74341 cacaaaattc taattatgta aaagtgttag aagtaaccca gcaaacgaaa caagatgaaa
    74401 caatccaaca aataccattc aataatttca caggggaaaa tgagacacta agtttccccg
    74461 tatcagaaac aacagaaaga atggtgtttc gcttcgcgca acaacctagc tttatcgaat
    74521 tcagcgcaga agctgccgga gaaaaactaa catttatcga ttcgaatgct gtagtcaaaa
    74581 cagtaaatga ttgttatttg tttgatggga aagaattacc agagttcgtt ctaacggata
    74641 tttctgggaa agaagtgact atccattgtc actatcgctt tatcggggaa ttgacaccaa
    74701 caatgaaaga attgttggaa acgattcgtc ccatggctga agtcacaaag cagctttctg
    74761 agaaaaatga cgaattagaa agagaaaatc atcatcttct tgaggaaaat acaagactag
    74821 accatacgtt aaaaacaacc acaaatcgct attgcacatt gttggaaagt gatgaatgga
    74881 caataaaaca tagactagga cgacgtaaga aagaaacttc gaaaaaaatc caagaaaaag
    74941 aactttctat atgtattgac gagaaaatct gggatgcaga aacaaaaata ttaaaaatca
    75001 tcggatgggg aatcgctaat tcggatcgcc agcccttgtc ctataagtta tcagctgacc
    75061 aatcgccatt tttcgaagca attcctgttt cacgagaaga agtcaatcaa gcagaacagc
    75121 tcgcaaaagg aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa gaacgttcat
    75181 tcttagtgga agcagtagct caaaatggcc aatcatggat cattgaaatg taaataatga
    75241 ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat
    75301 cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga
    75361 tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc
    75421 aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag
    75481 cggtttcaag gtacgtttat cagggaaaat gagaggaaaa gccatcttag acttccaaac
    75541 agcgagacat caaaatggca tagctgtgaa attaaacggt aaataccctt atgatgacgg
    75601 cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag
    75661 gactcatggc gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc caggatcgat
    75721 cgactatgca gagtggatca gtcgtcatga gaaaccagat ttgaaagagc aaaggaaaga
    75781 agcacaagga tttgcgtacc gtccattgat ttccatactt atgcctgtct ataacgttga
    75841 aatcaaatgg ctcgaaaaat gcattgattc ggtactggat caaacgtatg atcattggga
    75901 actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc tggaatccta
    75961 tcaagcaaaa gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac acatcagtct
    76021 ggcaactaat tctgcattgg aaatagcaga aggagaattt atagcactct tagataatga
    76081 tgatgaatta ccgccatttg cattatatga agtggcaaaa gtgctaaacg tacatccaga
    76141 actggatgtg atctacagtg acgaagataa aatcgatgca gatggcaatc gctttgatcc
    76201 gcatttcaaa gcagactggt cgcctgatac attgatggga aataattata tctctcactt
    76261 aggcgtatat cgaacaagca ttgttaaaga acttggcggt ttccgtaaag gctatgaagg
    76321 ttctcaagat tatgatttgg tccttcgtgt gacagaacaa atccctgcag atcatattta
    76381 ccacattgac cgagtcttgt atcattggcg gacgatccca ggatcaactg caagtaatgg
    76441 cgaagccaaa agttatatct atgattccgg agtgaaagca ttgacagatg ccctgtctag
    76501 aagaaacatc aaaggaagcg tacgtcctgg cagaatttct ggattttatg agattgctta
    76561 tgacgtcttg caagaggatt tggtcagtgt gatcattcca acgaagaatg gctatgaaga
    76621 tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt atgaaatcat
    76681 tattgcggat aacggaagta cagatccaaa aatgcaagaa ttatttgcag aatacaagca
    76741 tcagctgaaa gaccgcttta tcgtagaatt gattgacatt ccattcaatt attctcgaat
    76801 taacaattta gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac
    76861 agaagtgatt gagccagatt ggatgacggt gatggtttct tatgcacagt ttgatcgaat
    76921 cggctgtgta ggagcaaaac tgttttatcc agatgataca acgcagcatg caggagtatt
    76981 attaggaatc ggtggtgttg cagggcatgc attgaataac tatgatcgaa cacattgtgg
    77041 ttatttcggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat
    77101 ggtcaaggca gctgacttca atgctgttaa cgggtttgat gaaacattag aagtagcatt
    77161 caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca
    77221 tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata ctccagaaaa
    77281 acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat aaatggccag catatattgc
    77341 gcatgatcca ttttacaatg ataatttaac aaaagacgga atcggcgact tttcgttgag
    77401 accagattaa actataataa aaagctgtta tgttgtgatt cgctcgcaca taacggcttt
    77461 ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa gtcgaggttg
    77521 ggacagaagt gtttaactcc gagaaataag aagaaattca cgaaaattgc ttttcaaatt
    77581 tttgtgaatt tcggcttatt taagaagaag ttgcttctgc tcccaccgtt tattcgtttt
    77641 taaagaatga gacaaaagtg tttcttactt ttgtcccacg ctcttattca tgctcagagc
    77701 gccaatagat acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa agaatcgata
    77761 ttttatttta atataggcta ttttaaaata aatggtttaa ggtattcctc tacaaaagcc
    77821 actctatcaa ttatcatggg tttaaaataa gaagtgatgg agataaccag attgttctta
    77881 gcgtcaatat aaagaacatt tccactatta ccaattgctg aatatatttc gttctcagaa
    77941 tctaataccc accataaata gccatatctc atattttgta agtcttctaa agcactggtt
    78001 ctaggtttga acatctcgtt tatccacatg ggtggtacga tttgttttcc tttatacatc
    78061 cctttattta ggcataatac accaattttt gccagatcac ttgccgataa acacaaacca
    78121 tatcctgccg tcccgatatt tagtggatca caaaaccaga cattggtttt aggagactta
    78181 tccattgtaa actgcttatg ttcttcagct gtttcagcat aataattgac atggtttggt
    78241 atccctatag gatcaaatag atacttattg gcaaaatcaa cagttgttag cttacttgtc
    78301 tgatacaaca agccagttaa tatgtgtaaa cagacagttt gatagttgaa ttcgccggtt
    78361 ataccttttc ttcctcccaa taaatctagt gatgtttttg tccaatcttc acttgaacag
    78421 acttttgtcc atggatcgcc tttacatttg tagggtgctt tcattgttag caagtgcttg
    78481 attgtaatag cgtaaatagt tttctccccg cgtttcactt tatactctgg aaaataatct
    78541 aatactttat catcgatact tttgattaat ccttgctcta aagcaatgcc aatcagcaaa
    78601 gatacaatac ttttcgttgc tgacatgata tgacaagtat cgtctttttt ataaccattc
    78661 cattcatctg tataaacttc taccccatcc ttatatgcga caatttgaca tatatttggt
    78721 tgtcgaaaac caataaaatc atgcaactct tgtttattca taagatatta cctcactttc
    78781 ttctataagc ataatgtcat taaaaatcga tttcaatttt ttcgattctt cacgtaatac
    78841 aggtattctt atggactaga cagcgctttg ggaagatgga ttctctgaaa aatataaagg
    78901 ttcactttat gagtggataa aaaaagcagc aaatacttcg acttctttaa tttaccaaca
    78961 ttagtgaaaa tcgattcatc tggagatttg taatttagag tccttgtctt gattagaggt
    79021 gtataaagct ttttattaga catttttaaa ttccattaca atttgcttat aaactaggtt
    79081 tttaaaggtg actatggtaa gataatagag atattatttt gtgattagga gtgtatatat
    79141 gaataaaaat ggagaatgga gtgctgcacg acggatattc attatattaa tggatgttat
    79201 tgtatacaat gcagcaatat atagtagttt cttactgaag tttcgtggag agatcccatt
    79261 acgaaattta gaaactttcc aacactcagc aattattatt tcagtgattt ttattgcgtt
    79321 gaacattttg ttgggtgcat atgtttttta taatcgaatg atcagtgaca tcgtgtttgt
    79381 cacagtcatc ggtcaggtat tgatgacact agggatcatg gtagtgactt ttgcgggccg
    79441 ctggtttgct tttccacgtc ttgttatctt actttcattt gttctcggta cgcttttctt
    79501 aagtatctat cgtattctcg tatataaact ttatttacgc gtaagtcgag ataaaaaggt
    79561 cgtcattatt gggttagaaa aggatgtcgc accagcaatc caaaattttg gttcaaagaa
    79621 aaacaacaaa cataaagtcg aagcagtcat tcttgataac tattatgcaa acatcaaaaa
    79681 tatgattaaa gacatcgata ttgtctatct tgcttctcat atcgaagaaa ctgaaaaaat
    79741 caaaatctat caattagtta ctaaaaatga aaaaaaattg tttttgaata caacatttga
    79801 aaacttgaca ttgatcaatc caaatattat gaatttcgaa gatgaaagta ttattgaagc
    79861 atctggattt agaatccaac ctgaatatga actattcaaa cgtttgtttg atatcatcgt
    79921 ttcacttgtt ttattagtta ttgcttcacc atttatgtta ttcacagcaa ttttagtcaa
    79981 agtgacatca ccgggaccaa tcatctataa acaagtgcgg atcacaaaaa atcaaaagga
    80041 attttcaatc tataaattcc gctcgatgac cgccacagcc gaagcaaaat ctggaccagt
    80101 tttagctaag agcaatgatg cacgagtaac gcctgtaggt aaatttattc gagctgtccg
    80161 ctttgatgaa ttaccacaaa tcttcaatgt gctgaaaggc gatatgtcga tcgtaggacc
    80221 acgtcctgaa cgttctttct tcgtcgatca gttcaatgca gaaaaccctt attactactt
    80281 acggcataat gttcgtgctg gtatcactgg gtatgcacaa gtctatggaa aatatgtctc
    80341 tgattacaac agtaaattac gttttgattt actttatatc aaaaaatatt cattgatgct
    80401 tgatttaaag atcttgctgc aaaccatcaa gatcctattt gataaaatgt cctctcaagg
    80461 acttgaagaa gacgaaaatg tgatctcatt ggcagaaatt gcttttccga atgaaaaaat
    80521 ctttcgctag ttgggaggcg taaaaatgaa caatccgaaa ttcagcataa tcgtaccagt
    80581 atacaatgca gaagcaacta ttggtcgaac gattgatatt ctaaaagaat tggattatga
    80641 agagtacgaa gtcgttttag taaatgatgg atcaacagat caaacacagg aaatcattca
    80701 agaactgatt gaaaatgatg aacgttttga attgattact acgaccaatc aagggcccgg
    80761 attagcaaga aacgaaggaa taagacagtc aaaaggagaa tatctcttat tcttcgatgc
    80821 agatgacacg ccagtaaaaa cgatcttgtc agattatgat catttgctag aaaagagtcc
    80881 aaaagcggat cttgttgtta gttcgtttgt ttttcgtaca atggacgagg gaagaatcgt
    80941 ttccgaaaaa gaaaacttag ttacagaatg tcactatcta actcacgaag agtttataaa
    81001 agacatgtat cagttaatga atgaacagtt gatgtatgtt gtatggaaca aatgctatcg
    81061 tagcgatatt ctgaaaaata accaaattct atttaaaact tataatagtt gtgaggatcg
    81121 aatatttaac ttacattatt atgaatactg tcatcaagtg atcatgaacc caaaagtaga
    81181 atatatttat gaatttgaag gtgggaaggg gatcactaat caataccgac ctaacaagtt
    81241 cgaaacattt aaagaattct attcattagc aaatactgta acagacgata ttaataaaga
    81301 cggtatggcg gcgcttcttt taaaagggac aacttcagtt attttttcta tttatagtac
    81361 ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag cagatattga gtgatccagt
    81421 gatccgagaa gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa taacaaagtt
    81481 gctttacaat atgccagctc cactattttt atctgctgta aaagttggaa gcttcgttga
    81541 agtgaaaatg ccagggttaa tggctgcttt gaagcgagta tattagttaa tggaggaaaa
    81601 tataatgaaa agaattttgc attttcaggg acgaatgggt ttaggtggag cagagtcttt
    81661 catgatgaat ttatatcgta aaattgatcg tacaaactat caatttgatt ttttgattta
    81721 tgaagattat gcagatgttc aa
//
LOCUS       DAYYOA010000002        73951 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000002 DAYYOA010000000
VERSION     DAYYOA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.002"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..564
                     /locus_tag="ACHBKA_000086"
     CDS             <1..564
                     /locus_tag="ACHBKA_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9443815.1"
                     /translation="ILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVT
                     KHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTT
                     AHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFS
                     EVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            764..1078
                     /locus_tag="ACHBKA_000087"
     CDS             764..1078
                     /locus_tag="ACHBKA_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9443816.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1099..1476
                     /locus_tag="ACHBKA_000088"
     CDS             1099..1476
                     /locus_tag="ACHBKA_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9443817.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1486..2259
                     /locus_tag="ACHBKA_000089"
     CDS             1486..2259
                     /locus_tag="ACHBKA_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443818.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2281..3684
                     /locus_tag="ACHBKA_000090"
     CDS             2281..3684
                     /locus_tag="ACHBKA_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9443819.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3866..5050
                     /gene="mobT"
                     /locus_tag="ACHBKA_000091"
     CDS             3866..5050
                     /gene="mobT"
                     /locus_tag="ACHBKA_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9443820.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5047..5337
                     /locus_tag="ACHBKA_000092"
     CDS             5047..5337
                     /locus_tag="ACHBKA_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443821.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5334..5555
                     /locus_tag="ACHBKA_000093"
     CDS             5334..5555
                     /locus_tag="ACHBKA_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443822.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5597..6376
                     /locus_tag="ACHBKA_000094"
     CDS             5597..6376
                     /locus_tag="ACHBKA_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM9443823.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6437..6937
                     /locus_tag="ACHBKA_000095"
     CDS             6437..6937
                     /locus_tag="ACHBKA_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9443824.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6993..7631
                     /locus_tag="ACHBKA_000096"
     CDS             6993..7631
                     /locus_tag="ACHBKA_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM9443825.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7703..8098
                     /locus_tag="ACHBKA_000097"
     CDS             7703..8098
                     /locus_tag="ACHBKA_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9443826.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8082..10535
                     /locus_tag="ACHBKA_000098"
     CDS             8082..10535
                     /locus_tag="ACHBKA_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9443827.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10532..12559
                     /locus_tag="ACHBKA_000099"
     CDS             10532..12559
                     /locus_tag="ACHBKA_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9443828.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12556..13578
                     /locus_tag="ACHBKA_000100"
     CDS             12556..13578
                     /locus_tag="ACHBKA_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM9443829.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13595..14524
                     /locus_tag="ACHBKA_000101"
     CDS             13595..14524
                     /locus_tag="ACHBKA_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9443830.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14838..14885
                     /locus_tag="ACHBKA_000102"
                     /pseudo
     CDS             14838..14885
                     /locus_tag="ACHBKA_000102"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14901..15701
                     /locus_tag="ACHBKA_000103"
                     /pseudo
     CDS             14901..15701
                     /locus_tag="ACHBKA_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            15939..16175
                     /locus_tag="ACHBKA_000104"
     CDS             15939..16175
                     /locus_tag="ACHBKA_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443831.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16182..18134
                     /locus_tag="ACHBKA_000105"
     CDS             16182..18134
                     /locus_tag="ACHBKA_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9443832.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18206..18703)
                     /gene="dfrG"
                     /locus_tag="ACHBKA_000106"
     CDS             complement(18206..18703)
                     /gene="dfrG"
                     /locus_tag="ACHBKA_000106"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9443833.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18978..20105
                     /locus_tag="ACHBKA_000107"
                     /pseudo
     CDS             18978..20105
                     /locus_tag="ACHBKA_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            20203..20391
                     /locus_tag="ACHBKA_000108"
     CDS             20203..20391
                     /locus_tag="ACHBKA_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM9443834.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20449..20802)
                     /locus_tag="ACHBKA_000109"
     CDS             complement(20449..20802)
                     /locus_tag="ACHBKA_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9443835.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21333..21755
                     /locus_tag="ACHBKA_000110"
     CDS             21333..21755
                     /locus_tag="ACHBKA_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9443836.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21752..21982
                     /locus_tag="ACHBKA_000111"
     CDS             21752..21982
                     /locus_tag="ACHBKA_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9443837.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22480..22680
                     /locus_tag="ACHBKA_000112"
     CDS             22480..22680
                     /locus_tag="ACHBKA_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM9443838.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22707..23900
                     /locus_tag="ACHBKA_000113"
     CDS             22707..23900
                     /locus_tag="ACHBKA_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9443839.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23968..25533)
                     /gene="guaA"
                     /locus_tag="ACHBKA_000114"
     CDS             complement(23968..25533)
                     /gene="guaA"
                     /locus_tag="ACHBKA_000114"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9443840.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25592..26515)
                     /gene="coaA"
                     /locus_tag="ACHBKA_000115"
     CDS             complement(25592..26515)
                     /gene="coaA"
                     /locus_tag="ACHBKA_000115"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM9443841.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26711..27316
                     /locus_tag="ACHBKA_000116"
     CDS             26711..27316
                     /locus_tag="ACHBKA_000116"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9443842.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27624..28640
                     /locus_tag="ACHBKA_000117"
     CDS             27624..28640
                     /locus_tag="ACHBKA_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9443843.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28781..30082
                     /locus_tag="ACHBKA_000118"
     CDS             28781..30082
                     /locus_tag="ACHBKA_000118"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM9443844.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30128..30790
                     /gene="deoC"
                     /locus_tag="ACHBKA_000119"
     CDS             30128..30790
                     /gene="deoC"
                     /locus_tag="ACHBKA_000119"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM9443845.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30811..31209
                     /locus_tag="ACHBKA_000120"
     CDS             30811..31209
                     /locus_tag="ACHBKA_000120"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM9443846.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31275..32357
                     /locus_tag="ACHBKA_000121"
     CDS             31275..32357
                     /locus_tag="ACHBKA_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9443847.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32569..34131
                     /locus_tag="ACHBKA_000122"
     CDS             32569..34131
                     /locus_tag="ACHBKA_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9443848.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34124..35257
                     /locus_tag="ACHBKA_000123"
     CDS             34124..35257
                     /locus_tag="ACHBKA_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9443849.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35257..36213
                     /locus_tag="ACHBKA_000124"
     CDS             35257..36213
                     /locus_tag="ACHBKA_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9443850.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36312..37478
                     /gene="deoB"
                     /locus_tag="ACHBKA_000125"
     CDS             36312..37478
                     /gene="deoB"
                     /locus_tag="ACHBKA_000125"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM9443851.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37502..38320
                     /locus_tag="ACHBKA_000126"
     CDS             37502..38320
                     /locus_tag="ACHBKA_000126"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9443852.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38337..39044
                     /gene="deoD"
                     /locus_tag="ACHBKA_000127"
     CDS             38337..39044
                     /gene="deoD"
                     /locus_tag="ACHBKA_000127"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9443853.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39342..40028)
                     /gene="gpmA"
                     /locus_tag="ACHBKA_000128"
     CDS             complement(39342..40028)
                     /gene="gpmA"
                     /locus_tag="ACHBKA_000128"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9443854.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40200..40781)
                     /locus_tag="ACHBKA_000129"
     CDS             complement(40200..40781)
                     /locus_tag="ACHBKA_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9443855.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40914..41597)
                     /gene="rpiA"
                     /locus_tag="ACHBKA_000130"
     CDS             complement(40914..41597)
                     /gene="rpiA"
                     /locus_tag="ACHBKA_000130"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM9443856.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41916..42329
                     /gene="rpsL"
                     /locus_tag="ACHBKA_000131"
     CDS             41916..42329
                     /gene="rpsL"
                     /locus_tag="ACHBKA_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM9443857.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42413..42883
                     /gene="rpsG"
                     /locus_tag="ACHBKA_000132"
     CDS             42413..42883
                     /gene="rpsG"
                     /locus_tag="ACHBKA_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM9443858.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42977..45064
                     /gene="fusA"
                     /locus_tag="ACHBKA_000133"
     CDS             42977..45064
                     /gene="fusA"
                     /locus_tag="ACHBKA_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9443859.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45236..46423
                     /gene="tuf"
                     /locus_tag="ACHBKA_000134"
     CDS             45236..46423
                     /gene="tuf"
                     /locus_tag="ACHBKA_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9443860.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46830..47138
                     /gene="rpsJ"
                     /locus_tag="ACHBKA_000135"
     CDS             46830..47138
                     /gene="rpsJ"
                     /locus_tag="ACHBKA_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM9443861.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47169..47798
                     /gene="rplC"
                     /locus_tag="ACHBKA_000136"
     CDS             47169..47798
                     /gene="rplC"
                     /locus_tag="ACHBKA_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM9443862.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47826..48449
                     /gene="rplD"
                     /locus_tag="ACHBKA_000137"
     CDS             47826..48449
                     /gene="rplD"
                     /locus_tag="ACHBKA_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM9443863.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48449..48739
                     /gene="rplW"
                     /locus_tag="ACHBKA_000138"
     CDS             48449..48739
                     /gene="rplW"
                     /locus_tag="ACHBKA_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM9443864.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48778..49611
                     /gene="rplB"
                     /locus_tag="ACHBKA_000139"
     CDS             48778..49611
                     /gene="rplB"
                     /locus_tag="ACHBKA_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM9443865.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49654..49932
                     /gene="rpsS"
                     /locus_tag="ACHBKA_000140"
     CDS             49654..49932
                     /gene="rpsS"
                     /locus_tag="ACHBKA_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM9443866.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49955..50302
                     /gene="rplV"
                     /locus_tag="ACHBKA_000141"
     CDS             49955..50302
                     /gene="rplV"
                     /locus_tag="ACHBKA_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM9443867.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50316..50972
                     /gene="rpsC"
                     /locus_tag="ACHBKA_000142"
     CDS             50316..50972
                     /gene="rpsC"
                     /locus_tag="ACHBKA_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM9443868.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50975..51409
                     /gene="rplP"
                     /locus_tag="ACHBKA_000143"
     CDS             50975..51409
                     /gene="rplP"
                     /locus_tag="ACHBKA_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM9443869.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51399..51587
                     /gene="rpmC"
                     /locus_tag="ACHBKA_000144"
     CDS             51399..51587
                     /gene="rpmC"
                     /locus_tag="ACHBKA_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM9443870.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51612..51878
                     /gene="rpsQ"
                     /locus_tag="ACHBKA_000145"
     CDS             51612..51878
                     /gene="rpsQ"
                     /locus_tag="ACHBKA_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM9443871.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51936..52304
                     /gene="rplN"
                     /locus_tag="ACHBKA_000146"
     CDS             51936..52304
                     /gene="rplN"
                     /locus_tag="ACHBKA_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM9443872.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52341..52649
                     /gene="rplX"
                     /locus_tag="ACHBKA_000147"
     CDS             52341..52649
                     /gene="rplX"
                     /locus_tag="ACHBKA_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM9443873.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52677..53216
                     /gene="rplE"
                     /locus_tag="ACHBKA_000148"
     CDS             52677..53216
                     /gene="rplE"
                     /locus_tag="ACHBKA_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM9443874.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53235..53420
                     /locus_tag="ACHBKA_000149"
     CDS             53235..53420
                     /locus_tag="ACHBKA_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM9443875.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53456..53854
                     /gene="rpsH"
                     /locus_tag="ACHBKA_000150"
     CDS             53456..53854
                     /gene="rpsH"
                     /locus_tag="ACHBKA_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM9443876.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53886..54422
                     /gene="rplF"
                     /locus_tag="ACHBKA_000151"
     CDS             53886..54422
                     /gene="rplF"
                     /locus_tag="ACHBKA_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM9443877.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54583..54939
                     /gene="rplR"
                     /locus_tag="ACHBKA_000152"
     CDS             54583..54939
                     /gene="rplR"
                     /locus_tag="ACHBKA_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM9443878.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54960..55460
                     /gene="rpsE"
                     /locus_tag="ACHBKA_000153"
     CDS             54960..55460
                     /gene="rpsE"
                     /locus_tag="ACHBKA_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM9443879.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55475..55654
                     /gene="rpmD"
                     /locus_tag="ACHBKA_000154"
     CDS             55475..55654
                     /gene="rpmD"
                     /locus_tag="ACHBKA_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM9443880.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55698..56138
                     /gene="rplO"
                     /locus_tag="ACHBKA_000155"
     CDS             55698..56138
                     /gene="rplO"
                     /locus_tag="ACHBKA_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM9443881.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56138..57433
                     /gene="secY"
                     /locus_tag="ACHBKA_000156"
     CDS             56138..57433
                     /gene="secY"
                     /locus_tag="ACHBKA_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM9443882.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57495..58142
                     /locus_tag="ACHBKA_000157"
     CDS             57495..58142
                     /locus_tag="ACHBKA_000157"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM9443883.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58333..58551
                     /gene="infA"
                     /locus_tag="ACHBKA_000158"
     CDS             58333..58551
                     /gene="infA"
                     /locus_tag="ACHBKA_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM9443884.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58584..58700
                     /gene="rpmJ"
                     /locus_tag="ACHBKA_000159"
     CDS             58584..58700
                     /gene="rpmJ"
                     /locus_tag="ACHBKA_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM9443885.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58718..59083
                     /gene="rpsM"
                     /locus_tag="ACHBKA_000160"
     CDS             58718..59083
                     /gene="rpsM"
                     /locus_tag="ACHBKA_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM9443886.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59111..59500
                     /gene="rpsK"
                     /locus_tag="ACHBKA_000161"
     CDS             59111..59500
                     /gene="rpsK"
                     /locus_tag="ACHBKA_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM9443887.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59580..60518
                     /locus_tag="ACHBKA_000162"
     CDS             59580..60518
                     /locus_tag="ACHBKA_000162"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM9443888.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60546..60926
                     /gene="rplQ"
                     /locus_tag="ACHBKA_000163"
     CDS             60546..60926
                     /gene="rplQ"
                     /locus_tag="ACHBKA_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM9443889.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61198..62037
                     /locus_tag="ACHBKA_000164"
     CDS             61198..62037
                     /locus_tag="ACHBKA_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9443890.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62013..62882
                     /locus_tag="ACHBKA_000165"
     CDS             62013..62882
                     /locus_tag="ACHBKA_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9443891.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62872..63669
                     /locus_tag="ACHBKA_000166"
     CDS             62872..63669
                     /locus_tag="ACHBKA_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9443892.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63872..64618
                     /gene="truA"
                     /locus_tag="ACHBKA_000167"
     CDS             63872..64618
                     /gene="truA"
                     /locus_tag="ACHBKA_000167"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9443893.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64951..67620)
                     /locus_tag="ACHBKA_000168"
     CDS             complement(64951..67620)
                     /locus_tag="ACHBKA_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM9443894.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67858..68046)
                     /gene="rpmB"
                     /locus_tag="ACHBKA_000169"
     CDS             complement(67858..68046)
                     /gene="rpmB"
                     /locus_tag="ACHBKA_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM9443895.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68316..68678
                     /locus_tag="ACHBKA_000170"
     CDS             68316..68678
                     /locus_tag="ACHBKA_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9443896.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68730..70412
                     /locus_tag="ACHBKA_000171"
     CDS             68730..70412
                     /locus_tag="ACHBKA_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM9443897.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70529..72565
                     /gene="recG"
                     /locus_tag="ACHBKA_000172"
     CDS             70529..72565
                     /gene="recG"
                     /locus_tag="ACHBKA_000172"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM9443898.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72614..73615
                     /gene="plsX"
                     /locus_tag="ACHBKA_000173"
     CDS             72614..73615
                     /gene="plsX"
                     /locus_tag="ACHBKA_000173"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM9443899.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73737..>73951
                     /gene="acpP"
                     /locus_tag="ACHBKA_000174"
     CDS             73737..>73951
                     /gene="acpP"
                     /locus_tag="ACHBKA_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9443900.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAV"
BASE COUNT        24324 a        13153 c        15496 g        20978 t
ORIGIN      
        1 atattaaaaa atgaatttgg aatagaagtt aaagattatt ttattgactt agatttatta
       61 cagctaataa acagtgtgaa aagaatgtat agtggagacg acaacggtgt tgatttatac
      121 aaatttgtta ccaaacatat atttgttaat cttaatattg acgagaaaaa ttacccgtct
      181 atttttaagc actatctatt ttttattaac gaagtagaag atcggataaa aaataattca
      241 ttatctagta gtgtatctag ttttaagaag atgtttaaat ctcgagatgg tatcacactt
      301 actacagcac ataaagttaa aggaaatgag tatgatactg ttatcgctat taatcttcta
      361 aacgggaaaa ttcctcattg gaatgaaatt tttaatgttt ctgataaagg aattagttca
      421 gcgagaaaac tactatacgt aatttgttca agagcaaaaa ataatttatt tcttttctca
      481 gaggtgggtt attttacgag taaaggagac ttgctttatc ccactcaatt attagataaa
      541 atagaatttg attataatat ttaaaatacc taggtagtcg ttgttttaaa tatagtagga
      601 atcggacgct cagtacggag cgtctatttt tttacccaat tttgaaagga agtgaactta
      661 tgaaaataaa aaatcaagaa tcaaaaggtc gctctccccc ctttaaaacc atcacacaaa
      721 taagattcat tcaaacataa aaacagaaag gataggtaaa aatatggaac ttaaatatgt
      781 cattcccaat atggaaaaaa catttggaca attagaatat gctggtgaag ggaacattga
      841 acaacgtcga ataaatggca gaccaaccat tctctctcgt agttacaatt tatactcaac
      901 gattcaacga gcagatgata ttgttgtcat tcttccctcc gaagctggcg agaaacattt
      961 tgaatccgac gaaaaagtca aactcatcaa cccacatatt accgcagaag gttataaaat
     1021 tggcaatcga ggatttacca attacattat gcacgcagac gatatggtga aagcataaga
     1081 aaggaaaggt gaaaaaatat gagattagca gaaggtattg tgattgaaaa agaccagaca
     1141 tttggcacat tgaaattttc cgccttgcgt cgtgaggttc gtatccagaa tgaagatggt
     1201 acggtttccg aagaaattaa agaacgtacc tatgacttaa aatcaaaagg acaaggacgc
     1261 atgatacaag tcagtatccc cgcttctgtt cctttgaaag aatttgacta caacgcagaa
     1321 gtggaattaa tcaatcctgt ggcagatacc gtagcgacgg ctacttttcg aggggcagac
     1381 gttgattggt acgtgaaagc agacgatctt gtattaaaag gacagacaaa acctcatcag
     1441 aaatataaaa aagaagcgtc cgaaagtgaa aaataggtgt tgcttatgac aaacacacca
     1501 ttgattttga aagaaattcc gaaggatgaa gccatttctt ttattcggca ataccattat
     1561 tcaaagatac tccctagatt gtgtaaatac tttctaggga ttttttcaga agaaaaatta
     1621 ttaggcgttg tggaattagg ctggggtacg caacccctac aaaccatacg caaactcttt
     1681 cccgacagtt cccttcaaac cacagattat ttagaaattg gaaagatgtg ttttcttcca
     1741 gaaatgaatc aaaccaatta ttttggcagt caagctcttt cagctctaat taagtggttg
     1801 aaagaacata cggactgtca ttttttatat acactggcag atggaattga gggaaaatgt
     1861 ggctatgtct atcaagcgtc caacttcttt tactgcgggt atttcaagac aagtgtctat
     1921 cgtgataagc agtcatggga aaagatacac cctcgaagtg ctcgcctttt attagaggaa
     1981 aatgctcgct ttgaacaggt agagaaaaag cattggcttt cccaggcatt ttgcgaatac
     2041 aaagggattg agaaaatcaa tggacgcatg tttcgctatc tctatccgtt gacaaaagaa
     2101 gcgaaaaaac tgttaggaca cactctctat cgacgtcatt attatccgaa agaaaaaaat
     2161 ttacgctttg aaaagcgaat cgcttatcag aaatatgaag ccatttctca accaactttt
     2221 gataaacagg ctcgtatcta caatacacaa ttattttgat aggtggaaag gaggattttt
     2281 atgccattgc ttcatgacag aggagagcga atcaaagcca acgataagca cttggtctat
     2341 cattttgtac taatttcatt gctggtagtt tttctagtcg tcctactccc ttttcattgg
     2401 agagcgttac aatcggtgaa ctggcaagca gttcagttag atacattttt ctatcaactt
     2461 catacgccat acttttggag cagtattctc actgctcttt ttgtatgcgg acttatcagc
     2521 tggctttatt atcactatcg tatagattct ataaaacagt tagagcaccg tcaaaagtta
     2581 gctcgtatga ttttagaaaa tcattggtat gaagccgaag aaactcaatc agagggattc
     2641 tttaaagatt tacccagcaa gaaaaccaaa caacgaatcc gccattttcc taaaatatat
     2701 tatcgaatga aagatggact aatccatatt catgtagaaa tcaccttagg gaaatatcaa
     2761 gatcagcttt tgcacctaga aaacaaacta gaaacaggat tgtattgtga attagtggaa
     2821 aaaacgttga aagattctta tgtagagtat gtcctacttt atgatactat tggcaatcga
     2881 attggcattg aagacgtggt agctcaagat ggacgtgtca aattgatgga atcggtctat
     2941 tgggagttcg attctctccc tcacatgtta attgctgggg gcactggtag tggaaaatcc
     3001 tattttattc tcacattgat tgaatctctc ctgcatacaa atgcaaagct ttatattctt
     3061 gaccctaaaa atgctgattt ggcagactta ggggcggtca tgaataatgt ctattaccgt
     3121 aaagaagata tgttggcatg tatcaatcaa ttttacgaag atatgattga tcgcagtgaa
     3181 accatgaaac aacaccctaa ctataaaaca ggagaaaatt atgcttatct tggtttgtct
     3241 gccaattttc ttatttttga tgaatatgtg gcaatgatgg atatgctagg tcgtgaaagt
     3301 accaccgtta ttcataaact caaacaaatt gtaatgttag gacgacaagc aggcttcttt
     3361 ttaattcttg cctgtcaacg tccagacgca aaatatttta gtgatgggat tcgggacaac
     3421 tttaacttcc gtgtggcttt gggtcgtatg tctgaaatgg gctttggtat gatgttcgga
     3481 agcgatacac aaaaacaatt tttcttaaaa cccatcaagg gaagaggtta cgtggataca
     3541 ggaaaaagtg tgattagtga gttttataca ccccttgtac caaaaaggta cgatttctta
     3601 ggagaaatag gaaaagtcat tcaaaaaaag caatctgagc ctgtaccgca cgaagtgaaa
     3661 ggtacaggtt cagacttgct ttgattggtg tggcgttagc cacgccaatc ctttagcccc
     3721 ctctatctaa caggggggca caaatacaac tgaaaacagg caattctcat caaccaatgt
     3781 tgatggtaca aaggattgcg gaaaatatga aaggtgtaaa ctcaagggtc atagtttaca
     3841 tcttttttag attggaggtt tgcgaatgga aaacgaaaaa atttggatta aggaattgaa
     3901 agaaaagcga ctggcttatg gcgtttctca aaataaactg gcggttgctt ctcatattac
     3961 cagaccttac ctatcggata ttgaaacagg caaggcagtc cctactcaac aggtgaaaga
     4021 agatttactg aacgctttgg aacgcttcaa tcccgataac ccattggaga tgttattcga
     4081 ctatgtacgg attcgattcc ctaccaatga tgtggctcaa attatcggtg aggttttacg
     4141 tttgaatatg gattatatgc tacacgaaga ctttggctat tattcttatc ccgaacacta
     4201 tcgtttcggt gatattgttg tcctagtctc tcatgatgta agtaaaggtg ttttattaga
     4261 actgaaagga aaaggctgtc ggcaatttga aaactttcta ttggctcaac atcggagctg
     4321 gtatgatttc tttcaagatt gcatggaaca taaaggtatt ttcaaacgct tggatttagc
     4381 tatcaacgat aaaacaggga ttttgaatat tccagatttg gcaaagaaat gtaaacagga
     4441 agaatgcatt tccgtctttc gaagctttaa aaattatcgc agtggtgaat tggttcaccg
     4501 tgatgaaaaa ccatacatgg gaaacacact ttatattggc tcattaaaaa gtgaagtcta
     4561 tttctgtata tatgagaaag attatgagca atacgtgaaa aatgatgttc ccttagaaga
     4621 cgcagaagta aagaatcggt ttgaaattcg gttaaaaaat gaccgagcca cccaagccat
     4681 gaaagattta ttggcacatc aacaggcgga aaaaacagcc tttgaaatta tcaatcgcta
     4741 tatccgcttt gcagataaag acgacactaa acgtcggagt gattggaaaa ctaatgaacg
     4801 ttgggaatgg tttatcggta aaaatcgtgg agcattacgc ctaaccacac agccagagcc
     4861 ttattctttt gaacgaacat tgaactggtt acatcatcaa gtcgccccta cattaaaaat
     4921 cgcaagcatt ttagatgttc tcaacggtac aaccattatc tccaccatga ttcaggaagc
     4981 caaactgaca gaaaaacatg agaaattaat tgaacaacaa catctagcca tggaggattt
     5041 gattacatga gccttttaac aaaagaatta aagaagttag ggtttcagtg tggcattgag
     5101 tttcaagcgt atatacaaaa tacagggaaa tatacgtctc tcattattga gggaaaacgg
     5161 caagctggtg ataccatcta tacctatgat ttttacaagg tgagatttta taataattat
     5221 accaatcgag taaccgtcta tggagagcat ttaacccctt ttcaattatt aaggcgtgta
     5281 aaaagctata tctattatcg agaaaaatat cttaaggaaa ggaggacaat cacatgaatt
     5341 ttgggcagaa tttgtacaac tggttcttgg ataatgcaca gtcattggta ttgttagcga
     5401 ttgttgtcat tggactgttt ttaggtttta aacgagaatt ttcaaaactc attggcttct
     5461 tgattattgc tctaatcgcc gtcggacttg tgttcaatgc agctggcgtg aaagatgtgt
     5521 tattaaactt atttaaccga atcattggtg cttaacagat atttttcatg ataaaatagg
     5581 aagtatatat tttgttatga aaggatttat gaatatgttt acaaatgatc aatttaaatc
     5641 tcttttaatt aaaacattag aaagaaagag ctcttcaaat tattatgaag gaggtaacga
     5701 gattgtttct aaaatgaaaa tatcagaggt aactttagat gagactgacg attttactta
     5761 ttataaagga tataaaaagg gctggaatac gctttgcaca tatcttaaaa taagagttcc
     5821 ttttgatgat ttagactttt ttgaaagtca taaagatttg attacccaga cagcaagctc
     5881 catatatgat aaacaaggtg ataatgtatt agtggatact attttagtac cacttccaga
     5941 gaattacgaa gtaattaact ttagtcagtt gaaaattagt gatgtggttt cacaagctat
     6001 tgaagacgct gagtctttta tgagtaatgg tgaatatcaa cgtgcctttg acagagttca
     6061 cactgctttt catggatatt taattgaaat actaaaaaaa tatgaaatta cagttcctag
     6121 agacgaaaat ctatctaaat tatatagtag gatacaacaa ttaattgaaa aagaaattca
     6181 acctactgaa cttgctgata tagtgaagac aacgataagg tcatcaaatg gaatgattag
     6241 ctctcttaat gaagcaagaa ataggcattc acttgcacac cctaatacaa atattattgg
     6301 aaagagagaa gcaaaactaa taataggtat ttcaagtaca gttacagatt atattagcgg
     6361 ataccttgat aagtagcgtc attaactgat tgctactttt tttatgacta tttttataaa
     6421 aaggatgtga taagaattgg aaatgaaagt ctatgtggca aatttaggac ggtataatga
     6481 aggtgaatta gtcggagcat ggtttactcc accaattgat gaggaagaaa tggcagaaag
     6541 aatcggttta aatgaggact acgaagaata tgcaatacat gattttgagc ttccttttga
     6601 tgtggacgaa tacacgccga ttagtgaaat caaccgtcta tgtgaagcca ttcaagaaat
     6661 cgaaggtaca cctatttata atgaactcaa agaaattcaa ggcatgtggt ttagtagctt
     6721 ggaagaatta ctggaaaaca aagaggatat tcattgttat agtgattgtg attcgatgga
     6781 agatgtcgcc cgttattacg tggaagaaac aggacagctt ggtgaagtcc cttccaattt
     6841 acaaaattat attgactacc aatctttggg acgtgatatg gaaatagagg gtaattacct
     6901 agttacctct catggggtat ttgaatattg tcagtaagtt taacaatccg tgttataatt
     6961 aaattaataa atagcacgga gaggtataaa ttatggtaaa tgtatttgaa aatcttaaac
     7021 tattaaaact agacatgaaa gataaaggct gggtgataga ttccttttac tttcgttaca
     7081 aacagcaaaa ttatattgta cttgttaagc tatttgaaaa agaggaaaaa gttccagaat
     7141 atgctctgtt aaaactagaa tttttaaagg aaaacgattt ttcagatatg ctagctgtct
     7201 atgctaattc ggtaaaatta tatacagaca cgaaaacaat aagagaatat tttggtatcg
     7261 aatacagtag taacttggga gatgttttat ttcaatttag ccaaactctt gctcgattta
     7321 ttccgacaga agttagtgag aagaaaaatg aagaccaaaa agaagccatg tgtttctcat
     7381 taagtcaaag tgattcagaa gatcccagaa aaaaatattg tttttcagtt agaagaaatc
     7441 ctttaaaggg aaatggtgag ctgggaaaac gaagtccttt taatgacaat aaaacgagat
     7501 tgtatagacc cagcttatat gaacgattag ggtcagatac caatttaagt ttcagatatt
     7561 caatgaatcc agatgatgaa gaaactgatg aaggtattat tgcaaaatgg acaaaaaata
     7621 aaattgaata gttaaagcat tgaaagagca atcacctatt ttgattgttc tttttctatt
     7681 tttacggaaa ggacgtgaag acatgaaaaa aataagaagt tacaccagta tttggtcagt
     7741 agaaaaagtg atttatgcga ttaatgattt acaactccct ttcccaatta cctttacgca
     7801 aatgacatgg tttatcgttt ctttgtttgt cgtcatactc tttggcagtt taccaccact
     7861 ttctctgatt gacggagcac tactcaaata cttagggatt cccattgttc tcactgggtt
     7921 catgtgccaa aagacctttg acggaaagaa accctatgga tttctaaagt ctatcattac
     7981 ctatgctcta cgccctaaag taacctacgc tggcaaacaa atacgattaa aaaaagaaac
     8041 aatgaatgaa tcgattacga ttgtaaggag tgaacaacta gatggcatat ccgattaaat
     8101 atatggaaga taatctcgta tttaatcatg atggcgaagt attcgcttat tatgaattaa
     8161 ttccctataa ttacagtttt ttaagttccg atgaaaaaat tcaagttcat gataatttct
     8221 gtcaattgat tgcccaaaat cgtgatggca agattcatgc tctgcaacta gcaaccgaat
     8281 caagtattcg agccacacag gaacaatcga aaaaagaaat cactggacga ttaaaagaga
     8341 tagcttacga aaaaatagat gaacagaccg aagcgttaat ttctatgatt ggtgacaatc
     8401 aagtagacta tcgctttttt attggtttta aactctacct caatgaacag gaagtatccg
     8461 taaaaaatgt tgggaaagaa attcaaaaag catttgcgga attctttcat ggtgtgaatc
     8521 acaaattaat gggcgatttt gtttctatgc ctatggcgga aatcgaacgc ttttctaaga
     8581 tggaacagtt gttagaaagt aaattatcca gacgctttca gattcgtaaa ttggagaaag
     8641 atgattttgg ttatattatt gaacatcttt acgggcaaac aggtgtccct tttgatgact
     8701 acgactacca tttaccgaaa cgatacgaag ataaaaaagt gttggtaaag aaatacgata
     8761 ttctcaaacc cactcgctgt ttaatcgaag aaaatcaacg ctatttacgg attgaacagg
     8821 aagataccac tacttatgtg gcgtatttta caattaatag tattgttggt gaattggatt
     8881 tccctaatag tgaaattttt tattaccagc aacagcagtt tactttcccc gttgatacca
     8941 gtatgaatgt tgaaattgtc gccaataaaa aagccttatc taccgttcgc aataaaaaga
     9001 aagaactgaa agatttagat agccacgcat gggaaagcga caacgaaacc agtactaatg
     9061 tggtggacgc tttagaatct gtcgatgaat tagaaagtaa tttagaccaa accaaagaat
     9121 ctatgtacaa actaagctat gtggtacggg tatcagcaaa gaatttggac gagctgaaac
     9181 aacgttgtga tgaagtaaaa gatttttatg atgatctgtc tatcaaatta gtacgacctt
     9241 ttggcgatat gctaggcttg cataatgaat ttattccctc aagcaagcga tatatcaatg
     9301 actatattca atatgtcacg tcggattttt tggctggtct tggtttcggt gctacacaaa
     9361 tgattggtga aaattctggt atttacgtcg gttacaatct cgatacaggc agaaatattt
     9421 atctccaacc tagccttgca agtcaaggaa ttaaaggctc tgtaacaaat gcattagcgt
     9481 ccgcttttat tggttcatta ggtggcggaa aatcattctc taacaatatg ctggtctatt
     9541 attcagtact ctttggcgga caggcaacca ttgttgaccc aaaagctgaa cgtggtgaat
     9601 ggaaagaaac cttacctgat attgcccatg aaatcaatat tgttaatctc acgagtgaga
     9661 acgacaataa agggttactt gacccttatg tgattttaaa actcccgaaa gattcagaaa
     9721 gccttgccat tgatattctc acgttcttaa caggtatttc cagccgagat agcgagaagt
     9781 ttcctgtcct gcgtaaagct attcggagtg taactcaaag caaacaacga gggttgttat
     9841 tggtgattga tgaattgcga aaagaagata cgcccatcag caatagtatt gctgaccata
     9901 tcgaaagctt tgtcgattat gattttgccc atctgttatt cagtgatggc agtgtcaccc
     9961 aatccattag cttggaaaaa caactcaata ttattcaagt ggcagatttg gtcttacccg
    10021 attccaatag ctcttttgaa gaatacacta ccatggaatt attaagtgtg gcaatgctga
    10081 ttgtcattag tacctttgct ttagacttta tccactctga ccgttccatt tttaaaattg
    10141 ttgaccttga tgaggcttgg agttttttac aagtggcaca aggaaaagct ctatctatgc
    10201 gattggttcg agcaggacgt gccatgaatg caggggtcta tttcgttact caaaacgcgg
    10261 acgatttact agatgaaaag atgaagaata atattggctt gaaatttgcc tttcgttcca
    10321 ctgacctagt ggaaatcaaa aagaccttag aattttttgg tgtggacaag gaagatgaaa
    10381 acaatcagaa acgcttacgt gaattagaaa atggacaatg tttagtttca gacatctatg
    10441 gtcgtgtagg ggttatgcag tttcacccga tttttgaaga gttgcttcat gcctttgata
    10501 ccagaccgcc tgtgcgaaat gaggtggaag aatgacacaa aaacaaaaga aatttcttca
    10561 ctatagttgg ataacgctcc tttgtgtggg cgttatttta cttttattag gcacgctggg
    10621 caatgcagta caagcgacag gcttagttga tgaaaccatt gatacaagca atgaatactc
    10681 caagtatggt ttgaatcaat atcagcttga ttattatgtg gacaatagct ggggctggct
    10741 tccatggaat tggagtgatg gcataggaca atcggtgatg tatggattgt atgccattac
    10801 taattttatt tggacaattt ctctttatct atcgaacgcc acaggttatt tggtgaaaga
    10861 agcctatgcc ttagatttca ttagtcaaac atcccaagca attggcgaaa acatgcagac
    10921 gattgcaggc attacaccga atggcttttc cagttcaggg ttttatgtcg gtttcctcct
    10981 tctttttatt ctcattgttg gaatctatgt tgcctataca ggattggtta agcgagaaac
    11041 gacgaaagtg atacgagctg ttttaaactt tgtcgtggtg tttattctat cggcttcctt
    11101 tattgcgtat gcacccgatt atattgcgaa aatcaatgat ttttccaaag atgtcagtac
    11161 cgccagtctg aatattggga caaaaattgt tcttcctaac acggatagtc aagggaaaga
    11221 cagtgtggac atgatacgtg acagcttatt ctctgttcaa gtaaagcaac catggctatt
    11281 gcttcaatat ggctccactg atgtagaagc gttaggggaa gaacgtgtgg aaagtttatt
    11341 gtctaccagt ccagatacaa acaatggtga agatagagaa aatgtagtga ttgaagaaat
    11401 tgaagatcat gacaatacct atttaaccct accgaagacg atttccagat taggaacagt
    11461 cttctttttg tttgtattca atattggtat ctcgattttt gttttccttc taacaggaat
    11521 catgattttt tcgcaagtgc tatttattat tttcgctatg tttttgccaa tcagtttcct
    11581 attaagtatg ttgccgagtt ttgatggcat gggcaggcat gccattacca agttatttaa
    11641 tgtgattatg acaagggctg gtattacctt gattattacc gttgccttta gtatttccac
    11701 tatgctttat tcgctgtcag caagttctcc ctttttcatg attatgtttt tacaaattgt
    11761 gacctttgca gggatttatt ttaaactggg cgatttaatg agcctattca ctttacaaag
    11821 caacgactct caaaacatgg gcagacaagt gtttcgcaga cctcgtcaaa tgatgaatcg
    11881 acaaaccaga agatttcaac gcagtatgag taagattttt cgtggaaaag cagggtcaac
    11941 gaagcaaaag acaatgacaa caaagcagaa tcaaaccaat aaagctaacc atactcgtca
    12001 aaatgaacgg actacaccaa agaaagaaac agcactttcc aatgtgaaaa agcaacaagg
    12061 cggtatcggg caacgattag gtgaaaagac agcccatgta ttggacacga aaaatcgtct
    12121 agtcaataaa agaaagcagg caaaacaaca gatacaggcg acaccaaccc atgtcaaata
    12181 tgcccttcat caaggaaaag agaatgtcac tcataatgta acagacttta aacaatctat
    12241 ggcagatacc aaagctcaac gcaaacaaca gcgtatgaac cagcaggagc aaaaacgaaa
    12301 gaacattgct caaaagcgtt tggagctgga taaagcaaaa gaaaaaagac gaaaagaaaa
    12361 cccatcatct cctgttcaga ataaccagca gcatgttcgc aagcgtcagc aaaccgtcac
    12421 gcctgttttg tcacaaaaca ctaaacgtga aaatcatcag cgaccattga aaaaagagca
    12481 aaaacaaaag acaccaccga agcaattcaa tcagaggaag ctaaataaaa gaacggataa
    12541 gcgaggacaa caaacatgaa gctaaaaata acagtgattt gtagtgtggt cttctctctc
    12601 ttcttttttc tcttattatt cctagtcatt ttctttgcag atgatacgga cagtggcgaa
    12661 aacaacaagg attcttctat tccacaaggc ggtgtgaccg tttcaccaga agtgttggca
    12721 caccgtccgt tgattgaaaa atatggtaag gaatatggga ttgaagacta tgtttcttat
    12781 atcttagcca ttatgcaagt ggaatcgggc ggtacagcag aagatgttat gcaaagttcc
    12841 gaatctttgg gtttaccgcc caacagtttg agtaccgaag agtccatcaa acaaggggtt
    12901 aaatatttca gtgaattatt aaccagtgca gaacaacaag gagtggatat agattctgtt
    12961 atccaatctt ataattatgg cggtggcttt ttaaattatg tgaggagtca tgggaaaaaa
    13021 tatacctatg aattagccga acagttttct aaagagaagt cgggtggtca aaaagcagac
    13081 tatcccaatc ctatagccat acctgtgaat ggtggttggc gatataacta tggaaatcag
    13141 ttttatgttc agttggtttc acaataccta accgacactt ccccaacaga atttgatgat
    13201 gaaaccgtgc aagtcattat ggacgaagcc ttgaaatacg aagggtttcc ctatgtcttt
    13261 ggaggggcgt cgcctaccac ctcttttgat tgtagtggct taatacaatg ggtgtatgac
    13321 aaagctggca tttctctccc tcgtgtcgcc caagatcagt atgacgctac gcaagaaatc
    13381 tctatggaag aagcccaagc aggcgacctt atcttctttc attcaaccta taatgcaggc
    13441 acatatgtga ctcatgtggc gatatattta gagggaaatc gcttttatca tgcaggcgac
    13501 ccaatcggtt atggcgactt aagcagtcgt tattggcaag accatctgat tggggctaga
    13561 cgtgttattc ataactaaga aaggaatgaa tcaaatgatt ataaaaatcg agcgaaaaga
    13621 aaagaaggag aaaccaaaga aatctaaaaa accgagcaag caaagaaaag ttccgatgat
    13681 taaagttggc acgcataaga aattaacctt tgttttatgg gttttattga ttggtagtgt
    13741 cggatttgga atctataaaa attttactgc cattgacact catactgtcc gtgaaacaga
    13801 aatcattaaa cagcagattg tcgataccaa ccaagtagaa agttttgtga aatcctttgc
    13861 gaaagactac ttttcgtggc aacaatccca agaagccatt gataaacgaa atgaaaaatt
    13921 gacccattat ttgacggaag aattgcaggt attaaatgaa gaaatgattc gtaaggatat
    13981 tcctactagt tctagtgtga acgatataca ggtgtggcag gtttctcaag tgaatgagaa
    14041 cacctttgag gtactgtttt ctgttgaaca agttattacc gaagacaaag acaaggaaac
    14101 tatatcgtct agctttcatg tggttgtcca tatagatgaa tcagataata tggttattat
    14161 caaaaatccg acaatgagta agaaacctca aaaatcggac taccaaccca aacagctaga
    14221 aagtgaccat acagtagata cagaaacgat ggacgaaatc actagctttt tagaaacgtt
    14281 cttccagctc tatcctaccg ccacagaaaa agaattgact tactatgtaa gtaatcatgt
    14341 gttacctatg attaacaagg aatatgtttt cgaggaattg gtgaatccta tctttaccag
    14401 aaaagataac caagtaatcg tgaatgtcgc tgtgaaatat ttagatcaag aaacaaaggc
    14461 aacccaaatc tcgcaatttg agttgatatt agaaaaacaa gataattgga agattgtaaa
    14521 ataacaaata ttggtacatg attacagata ctttgtaatc atgtactctt tttgataaaa
    14581 aattggagat tcctttacaa atatgctctt acgtgctatt atttaagtga ctatttaaaa
    14641 ggagttaata aatatgcggc aaggtattct taaataaact gtcaatttga tagcgggaac
    14701 aaataattag atgtcctttt ttaggagggc ttagtttttt gtacccagtt taagaatacc
    14761 tttatcatgt gattctaaag tatccagaga atatctgtat gctttgtata cctatggtta
    14821 tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc cttttgggtt
    14881 tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct catgttgatg
    14941 caggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt acagaattag
    15001 gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt cagagaggaa
    15061 ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg aacatcatag
    15121 acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca gttttagatg
    15181 gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt atattatttc
    15241 atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt gaccaaaatg
    15301 gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc gaaattgtaa
    15361 tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc gaatctgaac
    15421 aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg tccggtaaat
    15481 cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat tgttctctgt
    15541 tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt atagaagtta
    15601 ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt
    15661 tcaaaattga atatacaaaa aaaagacaac gtcttgcata ttaagtagtt cagttttgga
    15721 gtacaaaaag tgaaaatgta aacctaaatg agtatcaaat tgcaactgaa tttgatactc
    15781 gttttttatt gttaaatcaa cgtttgttgt taatttacac cacaaaatgt aagttaaaat
    15841 gtatataaaa atgcgaacgt gaaataatta ttgtaggaat gcaaattggt gtgttaaaat
    15901 ggttacaatt tactaagcaa aaaggggtgc attttcctat gcaatttctt gagccaaaaa
    15961 ataagaacgc taagtctgtg gattgggaaa tatcagagca agtaagggtg atagttaagc
    16021 aatacgctga atatgcagag cgtactgaaa gcgaagcagt ggatgaattc ttattgaaca
    16081 ttttagacga taagaagttt attgaatgga ttgccaacaa gcgaagtaat aagcggattg
    16141 ttgaaaaaat gggtatcaaa gatagagtgg gttgatagac aatgaccaag cttaaaagat
    16201 tagccacgaa ggaagatgaa attgtagatg ttaaaattcc gatttcaaac gaagaattaa
    16261 aagatagggc taaacaatat gatttattaa ctccaaaacg atttgcaaca aggtataaca
    16321 aaatgttgtt cttgccaacg tcttttaaat ggaacggttc tgaatatccc attcaatata
    16381 attactgcat caatccgttt tgttgtaatt ttgggaaaga acagcacaaa ttcaaagatg
    16441 taaaagggaa accaagtcga tataaaatga ctggtagttc caaagataaa ggtcataaag
    16501 gtatgtactg caatgacaat cctattggca gaggtgtatc gcaaaactgt acagtaaccc
    16561 ctttatctaa ttggtctgtt gttgaagaaa ttaaacgcct tatagaaatc aatagcatcc
    16621 aagatgttga gccagattat caatttcata aagaaggctg ctcggaagaa gaatcaaccc
    16681 cattcaatga gccgaagcaa ttttacaaaa gggggaaaag taggggcaaa tctcaacgct
    16741 atcaatgtaa ggcgtgtaag aagttcacta atgttctacc aaaaagagaa gaaaccacta
    16801 cttatcacca acaaaagaac accattcttc caatgttcgc taagatggtt gttggaaggg
    16861 tttcagtcag tcgtacttgt gatattttag gaattggagt tggaacctac tatcataagc
    16921 tggagtggct gtataggcgt tgtttggagt ttttagaacg atatgagaca caaccccttc
    16981 aaacaaagaa atttaacgag atgtggctta atacggacaa gatgcactac tacctaaata
    17041 acgtccgtaa gaaaggacaa ggttcaaaga aatatacggg gtttgaagac cttaatatgc
    17101 aaacgtatat agttgtatct gctgaggtct tatcaagata tgtgttccgt tctgatgttg
    17161 cctatgattg gaatatttca atggatgaat taaatgaaga tacaagaaaa ttcaaagaag
    17221 accatctcaa tacttttagt cgtaaaaatg ataggttaga ttggtcttat tatccacaag
    17281 aaccatccgc taacgatagc gaaaatagga atgcttatct tcacgaatta gggaaaatta
    17341 ctaataggag tagatttgtc gatggtttaa atgttgatgc tccttatact acaactgctc
    17401 actattggtt aattaaacaa atggttaatg ccgacgaatg gcgaatgatt agtgatgatg
    17461 acttctctat tagaaacgcc ttttatagag tattcacgaa agaattaagg ttgagcgatg
    17521 cacatcattt tatttgccaa gttaacaaga cgaaaagcag aaaacaatgc ttaaaagaat
    17581 tcggacaagc aaaagcagaa ctgctcgatt ggggagatat cagaggattc aaaacaaagt
    17641 ttctacggac tttggcatct cattatctaa ctgaattact aacaagtcat caattccacg
    17701 aagaggctat tagtaaagat ggagaaagat atcgcaaata tgccgataac cctatcaaac
    17761 atccattagc cactaaggat aaaggatttt attcagttga ttgtagaacg gatttatctg
    17821 ctctggaacc aaacgaaatc gcaaagatgc tattaaatgt taatgaccat tcgaccaata
    17881 gctttattca acagataaga cgttatatat catctcttga aagaccatta acaacagctc
    17941 gtggtgataa gaagagctat atttacgcta acttcaatcc gaagtatgct caatttgcga
    18001 ttacaattct aagaacttac tacaactttt gtcgcccttt taaatcagcg gataaaaagg
    18061 ttttgacacc tgctcaaaga ttagggatta cagataaaca atttgattgg aaagatataa
    18121 tttatttcaa atgacaaaaa gagcttggag taatcccaag ctcttgtgct ttatctttac
    18181 tgttcttctt caattaacgc acccgttaac tcaataagtt ttttctttca tatacatgaa
    18241 aatagtagtt atacggattt ttatcattct ttatcccttt ttgggcaaat acctcattcc
    18301 attcctcata attcacttct ggaaaaaaag tatctccttc gaattcatga tgtatttttg
    18361 tgatgtacat tttctcaaca taagggaaaa acaaattata aatctgttct cctccgaaaa
    18421 taaaaatttc ttcttcgttt ttacataact caaaaacatc ttctattgaa tgaacaattt
    18481 cacaaccatt aaaggtaaac cccttatctc tcgtcagaat aatatttctt ctgtcaggta
    18541 aggctcttcc gattgattca aggttcttcc tacctaatat tatcggatgt ccctttgtag
    18601 tatttttaac atattcccag tccttgggaa tcctccaagg aatgtcattc tctttgccaa
    18661 tcactctatt cttatccatc gcagcaatca aagaaacttt catttataac acagcctttt
    18721 taactaattt accatctaat ttcttatttc tatcaaaaaa ccttttctca ttatcctgcc
    18781 cgttagttga ataagcaatt ttttctataa gttatattat cataatacca caaaatcaca
    18841 aattttcatt caattatcat tttcaattgc attttataaa tcattttgta tgtaatttgg
    18901 tatgtaagtt aaaatgaaaa aagtagtcca ttttgaagta cattttgtca ttttgtactc
    18961 caaaactgaa ctacttactt gcatatatac gcctttatag tggagtacta catttacgag
    19021 attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat acttcaataa
    19081 atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt ttgcaaaatg
    19141 agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag agaaaaaaga
    19201 ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct gaacagagag
    19261 aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta cgatattacg
    19321 tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa atggaagtga
    19381 ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag cctacagtca
    19441 tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa gtgccgccaa
    19501 atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga agtggaatgc
    19561 agtatgagag ctcggtttct cttggatact taaatcaatc atttcaaaat gcagttatgg
    19621 aagggatacg ctatggttgc gaacaaggat tatatggttg gaatgtgacg gattgtaaaa
    19681 tctgttttaa gtacggttta tactatagcc ctgttagtac tccagcagat tttcggatgc
    19741 ttactcctat tgtactggag caagccttta gaaaagctgg aacagaattg ttagagccat
    19801 atcttagttt taaagtttat gcaccacagg aatatctttc acgggcatat aacgatgctc
    19861 ccaaatattg tgcaaatatc gtaaatactc aactgaaaaa taatgaggtc attattattg
    19921 gagaaattcc tgctcgatgt attcaagatt atcgcaatga tttaactttt tttacaaatg
    19981 ggcttagtgt ttgtttagca gagctaaaag gatatcaggt taccactggc gaacctgttt
    20041 gccagacccg tcgtctaaat agtcggatag ataaagtaag atatatgttc aataaaataa
    20101 cttagtgcgt tttatgttgt tatataaata tggtttctta ttaaataaga tgaaatattc
    20161 tttaatatag atttgaatta aagtggaaag gaggagattg ttattataaa ctacaagtgg
    20221 atattgtgtc ctatttgtgg aaataaaaca agactacgaa tacgagtgga tactatactt
    20281 aaaaatttcc ctttatactg ccccaaatgt aagaacgaaa ctttaattaa tgttcaaaaa
    20341 atgaatataa taacaatcaa agagccagac gccaagacgc agagccgata atttgagaaa
    20401 tgaaactctc atcttatcgg ctctttttgt ttatctgaat tttactgact agccttcaat
    20461 atttcttgct tcattgatac cttttgctag tgcttccatt aaggatagtt ctttgtctgt
    20521 aaagctatcc atgtattttt ctacctgtaa ccttcgagtg cttttaatca aattatctgt
    20581 aggtaagaaa aattcatcaa tagatacatg gagtaatgat acaagatcat acagcacttg
    20641 tgtacttggg tgttgtcctt tgttttctat atttgttaaa taacgtgggt caatttcaat
    20701 cattgttccc acttgttcac gagtgagacc ccgtttcatt cgagcctctt taatcgctaa
    20761 accaacggct ctaaaatcgt atttatcttc ttttttacgc atactaaatc acctctatac
    20821 attttactgt tcctatataa ccataaacag gtatagaaaa acatgttata tagtttatga
    20881 gttcctatag aacactgtgg aatttattaa tgcgatgaat ccaatttcaa atagaaaggt
    20941 cgctgatttt atttacctat acaactttat aaaatttagt catactccta gaaagtagat
    21001 aaaattggcg acttaatgga gggaaaagtc gccatgagaa agataatctg tcatctagtt
    21061 ctcttaatta agttcttatc aaaaaaactt atccacctgt actggataga tatgaccatc
    21121 aatgtggttt tgcagattac atagatagaa aaatcaaagc atatgaacag agaaaacaat
    21181 ctgtttatgt gcttttttgg tcacttaata aatatctttt aggatttctc ttcatttatg
    21241 caacaaatcc cccgttcgtg tcggggttta gtatgaaggg agataaagaa gcacatgcca
    21301 ctccctttca cgacagaaag ggggtgaaaa gcatgaaacc atcttctttt caagaaacca
    21361 tagaaaatca gtttgactat atctgcaaac aagttattga agatgaacga aaagattact
    21421 tcaaacatct agacagacta tcagcaaaag aagtctcgtt ttctgatata gataactacc
    21481 ttgtcaactc tttttctact gttgatacgt acgtcactga tttccaacta tttaaattat
    21541 ttggattaag aatcaatgtt gaaagtgatt tattaagtga agccctgcgt catctttcag
    21601 agaaaaaacg gaacattatt ttacttcatt actttatgga tatgagtgat gtggagattg
    21661 cggagttact aaatttgaat cgttcgactg tttatcgtca tcgaataagt ggactagcca
    21721 tgattaaaga attcatgaag gagtgtaaag aatgaaaaca gcttatccaa gggttccctt
    21781 ccccctcatt gtaaaggcga cagatggcga cgtggaagca attaatcaga ttgttaaaca
    21841 ttatcgaggg tatacctcaa aacgttcatt acgtcgcatg acagacgaat atggaaattc
    21901 tcatatggtt attgatgaaa ccctacgtgg cagaatggaa acgaaactca tctctaagat
    21961 tttatcgttc gaaattaaat agtcccattt tttcgtggaa gcgtgtcgaa tcgtatccac
    22021 gcttcccgat ttgggaggtt tgttattcca tcaaagcata ttgtgtgttc agtaggtttt
    22081 gataggctaa caagccaaac gttctttgac aaccaaataa aggcaatcga atacgtttcg
    22141 gtaagaaaag agctgatgaa ctaacacgcc atgacctaca aaattagtga gcgattcatg
    22201 ttagtaattc gaaagtaatc tttagcagga ttgctgccat gactttctta tcgtgataat
    22261 gatactcccg tatagccata gtccgagcgt tagaagcgtc acaggcaatg ggtacggcta
    22321 catgagaacc atgcagaagt gaaattcctg tgagctttgc tagagctgtt cgattgctaa
    22381 gatattcttc tcttgtcagt gtattattat aaaactattt gatataatta taggtgaata
    22441 ttggagtttt cctagcctat aattgggaaa ggagtaataa tgataacggt cacaaaaaag
    22501 gatttagttg agctaggata tggaacttct ttttcagcag acatcataaa aaaagcaaaa
    22561 gaattgatga ttacaaaagg gcatatctac tatcaatctc gaaagctaga tagagtccct
    22621 cgtgaagctg ttgaagaaat tctaggaatt cggttttcaa atgaaaacag gtctgagtag
    22681 tttttgcaca gtaaggagta aaaactatgg ttaaagaccc aataaaaaaa gcaaagaatg
    22741 gaacctatta ttttcgtgca aatctaggtt atgattctaa agggaaaaaa attcaaaaat
    22801 atcgtagtgg attcactact aaaaaagaag caagagaaga atattcgaaa ctgctattga
    22861 tgaaaccaga agaattaagt gagaataagg ataaaatgac gtttaaacat tatatctttg
    22921 aaattttcct tccatggtat aagacacagg taaagctcag aacatatgaa aatcgcttac
    22981 caactattaa gaagcatttc tcttattttg ataaaatggc tgtttccgat attgagccta
    23041 ttgatgtgca aaattggcaa ctaaaattat caaagaagtg taagtcttct tatgtaagag
    23101 ctgttcaagg actattttca gttgctatgg atcgagcaat tgtccttggg ataaccacca
    23161 caaatccatc taagattatt ggcaatgtga aaaagcaaaa gtctaaaatt gaattttgga
    23221 caaaagaaga atttgaaaaa gtaatctctc taatttacaa agaagattac tatcaacact
    23281 ttttatttat ctctctgtgg tttttattta tgactggaat gcgaattggt gaagctactg
    23341 ccattcaatg ggaagatatt gattttgact caggagtact aaccatcaac aaaacactct
    23401 attacaagaa tcaatcgaat tatagatttg ttgaaccaaa aacaaaggcg agcgttcgtc
    23461 atattgtatt agataaatgc acattaactt atttatccga atggaaggaa gtacaacaaa
    23521 atctaattca gacagacttt gtaatgagct ataacggtat tcctacccaa aaacatacac
    23581 tatcatatgc tattgaacgc tatgctaagc tggctggtat ccatcgaatc agaatccatg
    23641 ggttacggca ttctcatgct tccttgttaa tcagtatggg agaaaatcca cttatcatta
    23701 aagatcgtct aggacacgaa gatattgaga cgactttagg aacgtatgga catctttatc
    23761 ctaacagtaa tttcgaggta gctaacaaat taaatggagc catttccttt aaagaagcaa
    23821 aacaaaatta tgatacttca ccaaagaatc aatttactgt agactacttg cgaaacaaag
    23881 aaatgaaaaa agtgcaataa aagtgcagta aaaagataag aagagccaga aacccttggt
    23941 ataagaggat ttctagctct cctacaacta ttcccactcc actgttgctg gtggtttaga
    24001 agtgatgtca tacacgatac ggttgacgtg ggctacttcg tttacgatac gtacagagat
    24061 tttttgcaag acttcccatg ggatacgggc aaagtcagcg gtcattccgt cgattgaagt
    24121 tacagcacgg ataccgactg tgtagtcgta agtacgtccg tcacccataa caccgacaga
    24181 gcggatccct ggaagaacag tgaaatattg ccagatatca cggtcaagtc cagcagctgc
    24241 gatttcttca cgcaagatag catcagattc gcgtacgatt tctagcttgt cttcagtgat
    24301 ttcgccaaga acacgaatac caagaccagg accagggaat ggctgacgcc aaacgattga
    24361 atcaggcatg cctaactgag tccctaattc acggacttcg tctttgaata atgtgtttaa
    24421 tggttcgatc aattcaaatt gcatatcttc tggcagtcca cctacattat ggtgagattt
    24481 gatcgtctgt gctgtttctg taccagattc gataacgtca gtatacaacg ttccttgtgc
    24541 taggaaagag acgccttctt caccagcaag ttttgttgct tcgtcatcaa aaacatatac
    24601 gaattcattc ccaatgattt tacgtttttg ttctgggtca gatacacctt tcaacttgtc
    24661 taagaagcga tctcttgcat cgactttgat gatgttcaag ccgaattttc cgcctagact
    24721 ttccattact tgttccgctt cgcctttgcg tagcagaccg tggtcaacaa agatagatgt
    24781 caattggtca ccgatcgctt tttgtaaaag aaccccaacg acactagaat caacgccacc
    24841 tgaaagacca agcaagactt ttttgtcacc aacttgttca cggatttttt ctacttgcat
    24901 atcgataaag ttatccattg tccagttacc ttcacagtga caaacatcaa aggcaaagtg
    24961 gcgcagcaat tcgattccgt attcggaatg acgtacttct gcatggaatt ggataccata
    25021 aaaattacgt gttgtgtctt ggatagaagc aatcggacaa tctttgcttg ttccgactgt
    25081 ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg ctcatccata ctgtttgttg
    25141 ttttggtgtt tctttaaaca agacagcttg atcgcttgtt acttctaaca aggctttccc
    25201 atattcgcgg tttttagcag gctctacgga accgccaagt ttgtacgtga tcaattgcat
    25261 accatagcaa atccctaata caggaatacc taaattgaag atctcaggat cgatcccaaa
    25321 tgcattctca tcatatacac tgtttggtcc gccagaaaga atgattccct ttggattcat
    25381 cgcttttact tcttctgcag taatgcggtg gctcaataat tcggaaaaga ctccaagatc
    25441 acgaatacgt cttgtgatca attggttata ctgactaccg tagtctaata cgataatctt
    25501 ttcaacgttc gtcatatcgg caacgtttgt caaatttatt acccctatcc ctttaaattt
    25561 tatctcaaaa tagtcaaact attttaaaat gctaatattt tcgcatgaaa atcgaatcga
    25621 tgatatgatt ttcagtttta tggagaatta tatcggctct tcctcgtgta ggcaagatat
    25681 attcctgtaa atttttcaaa ttcacatttt tccatacatc ttttgccatt ttaaaggctt
    25741 cttcacgatc tccgatagca aaacgataat aataattctc tggattttgg aaagccgtat
    25801 ccagtaacgc accaaaacga tccaaatacc atttttcgat cagcacggga tcggcatcga
    25861 catagacaga aaaatcaaaa aagtcgctga cgtagatttg ctgattcgca ggtaattgaa
    25921 gcgtattgat cccctcaacg atcaagatat caggctgctg gatcaattcg tacttgtctg
    25981 gaatgatatc atacacatca tgtgagtaaa caggtacttt tatctcttcc tgaccagatt
    26041 ttacacggtt caagaaatcg atcagcatct ccatatcata actttcagga aatcctttac
    26101 ggtccatgat tccacgctct tttaatatac gatttggata aagaaatccg tcagtcgtga
    26161 tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat cgtctgcagc agtctggctg
    26221 ttgtactttt tccaacggct acacttccgg cgatcccgat aataaaaggc ggtacttcta
    26281 cgtatttatg aagaaataaa cctttactca agaacaaaga ttcaaattct ttcatataaa
    26341 gataaatcag atgagcaaga ggtacataga tatctcgaac atcctgcatg gaaatgcggt
    26401 cattcaaact tttgatatta tctagttcat cttgtgtcaa aggtgcttgc ccattacgat
    26461 aaaattctcg ccattcttct cttgccacac ggtagtagtt cattggatca ttcatattag
    26521 cctcctgcgt tctcccatca atggaactat tttaacacaa aataaacgca aaccccacca
    26581 cttaagagag attgccgatc tttttgtata atctttataa tagaaagaaa cggagtctta
    26641 ttcttgactt gcgcacggat acataaaagg atagactgtt gtcatgaaag aaatgagaag
    26701 gatggaagac atgacaaatc attactattc tgaacgccca gatacggcgc acgattttga
    26761 acaatgggct tttgagttaa aaggcagaac ctttcaattt gtaacagatt ctggtgtatt
    26821 ttcacgtgaa acagtggatt atggttcacg tgtcctgatt gatgctttcg attgggaaga
    26881 gttacctgat gaaggaaaga tcttagatgt gggatgcggg tatggaccga ttgggttagc
    26941 tttggcattt gcgacacagc ggtttgttga aatggtcgac attaatgaac gagcggtaag
    27001 tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat gttgatatcc atcaatcgaa
    27061 tatctatgaa gcagttcatg aagaaaccta tgcagctatc gtaagcaatc cgccgatccg
    27121 tgctggaaaa aaagttgtac atgaaatttt gacaggcgca tatcctcggt tgaagaaggg
    27181 cggtacactg acaattgtga tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat
    27241 ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat aaaggatatt atatcatcaa
    27301 aagtgtgaaa gaataactag ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt
    27361 gcatgtacgg gtacgtatca gttctttttt tgttgagaag tttttaaaaa caaagaacta
    27421 ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt tttttacgat aaaataaata
    27481 agatacagaa cttgtataga aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa
    27541 aacatacttt ccttttttct ctattctttt cttgggggtt ttggtacact gatagggaat
    27601 ttagatagaa gggagccaat aggatgaagc tgaccatcag agatatcgca gaaatggcag
    27661 gcgtatcagt aacaactgtc tcacagattt tgaataacaa aggaagcaga tttagtgata
    27721 agacaagaaa aaaagtgttg tcgatcgtga atgaacatca ttataagccc gattatttcg
    27781 cttcaaatgt gatcaatcga cattcaaaga ccatcggaat gatcgtccct gatgtcactg
    27841 atttcttctt ctccaaactg atcgaaggtg tagagagcta tttgaatcct ttgggctatg
    27901 tgatcatgtt atgcaattct cgtcatagcc aagaaaatga gatcaagtat ttacaagagc
    27961 tttcccaccg ctcagtggac ggaatcttgc tggccacacc aaatatttta cctgagaaat
    28021 atgcattaga cagcggtttt tatcaaaaaa tgccaatcat tttaatcgat cgcggattga
    28081 atcgtcggga taacggacgg cttatcgtaa aggaatatga gggcgcctat caagctgttt
    28141 cgctgtttat tgaaaatggc catacgaaga tcggcatgct gaaggaaacc actggatact
    28201 atcaattaga agaacgtttc aacggatatc gtcatgcgct gaaagatcat ggcctgcctt
    28261 ttaatgggaa atttgttgaa catggagagt tgactgtcca aggtggatat actgcatcaa
    28321 aaaaattact caaacataaa gatatcacgg ctattttttg cgggaatgat gccatggcga
    28381 tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat cccagaagat atctcagtca
    28441 taggattcga tggattaaag ctgtcagaat acatgattcc gcaattgacg acagtgcagc
    28501 agccaagttt tgatattgga ttttatgctg cacgtttcct gatcgatacg atcgaatttc
    28561 ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa
    28621 aagtattgac aaagggctaa ctcggcggta tattgcttat gtaagcattt acaaaggcta
    28681 gttagtgctt tttttatttt tagtaaacca gtttactaaa gtggtttacc tttcctaaaa
    28741 ataatgtata ataccgaatg aagacaagga ggatcaggca atgcgaatgg tggatctgat
    28801 tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa
    28861 ctatacgaat ggaaagattc cagattatca agtcagtgca ttattgatgg caatttttta
    28921 tcaagacatg acaaatgaag aaatcacgaa cttaacattg gcgattgcta attctggtga
    28981 tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat aagcattcga caggtggcgt
    29041 tggtgacacg accacattga ttttagcccc cctagtagct agtgtcggag tgacagtggc
    29101 taaaatgtct ggacgcggtc taggttacac aggtggtaca ctagacaagc tagaagcaat
    29161 tcccggattt cagatcgagc tttcggatga agaatttgta cgcattgtta atgaaagtaa
    29221 agtagcggta attggtcaat caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt
    29281 gcgtgacgtc acagcgaccg ttgattctct tccgctgatt gccagttcga ttatgagtaa
    29341 aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg accactggag acggtgcttt
    29401 catgaagaac atcgaagatg caagacgtct ggcaaagacc atgacgagca ttgggaaact
    29461 ggctaaccga gaaacagtgg cggttatctc agatatgtca gaacctcttg gcgaagctat
    29521 cggaaatagt ctagaagttg tcgaggcaat cgaaacatta caaggaaatg gtccagaaga
    29581 tctagtagaa atgtgctacg ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa
    29641 gacaaccgat gaagcaagaa cgttgcttca agaagctctg gaatcaggta aagcgttagc
    29701 aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca gcgatcgtgg aacatccaga
    29761 acgaattttg actgcacgct acacaatgga gcttcctgct aaacaaagcg gagttgtgtc
    29821 aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac
    29881 caaagaagaa gatattgatc acgctgttgg attgaaactg cataaaaaaa ttggtgacac
    29941 tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac gacgaagaaa tcacttctgt
    30001 cattgaatta ttatataaaa atattgaaat tggtgaatcg gcaatgaagc caacgttgat
    30061 tcatgacatc atcacagaat aaaggaaaaa agcggacttt accgttttaa agaaaagggg
    30121 aaaagaaatg gaactaaacc gaatgatcga ccatactatt ttgaaagcag acgcatctaa
    30181 agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac catttttatt ctgtctgcat
    30241 caatccagca tgggttgcgc tagcgaaaga acaattacaa ggtgagccag tagccgtatg
    30301 tacagtaatc gggtttcctc taggagcaaa cacacccgag acaaaagcat ttgaaacaac
    30361 agatgccatc aataacggtg cagatgaagt cgatatggtc atcaatatcg gtgctttgaa
    30421 atcaaaggat gatcaacaag tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga
    30481 tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta gatagagaag aaatcatccg
    30541 agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc
    30601 aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt gaaactgtag gaccagaaat
    30661 gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc
    30721 tggtgctaca agaatcggaa caagcgcagg agtagcaatc gtatctggtt caactggtga
    30781 aggctattaa ttttttcaaa ggagaagctc atggcagaac tcaaacaaga atggatcaac
    30841 acagctattg aagcgttaga taaagcctat gttccttatt cccattttcc ggtaggcgct
    30901 tgtctcgtaa cggaatcagg caagatttat caaggcatca acattgagaa tgcatcattt
    30961 ggattaacga attgtgccga gcgcacagct tttttcaaag cagtatctga aggagaaaaa
    31021 acatttacgc atctagtcgt agcggggcac acccccgatc ctatctctcc ttgtggtgca
    31081 tgtcgacaag taatggcaga attctgttcc cctgatatgc cggttacgct agtaggagac
    31141 aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa
    31201 gatctataac tcatgttcat tcagtcagct gactgttgaa aaataaaaca attattttag
    31261 gaggctttac agaaatgaaa aaagcaaaat tatttggttt aggtgctgtc gcactagcag
    31321 cagggttatt tttaggtgca tgcggcaaca acggctctac tgattcatcc ggtggcaaaa
    31381 gctcatcaga cacaacaact gcagcattga tcacagacac aggtggtgta gacgaccgtt
    31441 cattcaacca gtcagcttgg gaaggactag aaaaatgggg gaaagatcaa ggactttcac
    31501 gaggaaatga tggcttccaa tatttccaat cttcaaatga atcagattac atcccaaata
    31561 ttgaccaagc attgaatgct ggattcaaaa cgatttttgg tattggttat aaactaaaac
    31621 cagcgatcca agaacaagca acaaacaata caggtacgaa ctttgttatt atcgatgatg
    31681 tcatcgatgg attagataac gttgtatctg caactttcaa agacaacgaa gcttcttatc
    31741 ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt agtcggcttt atcggcggtg
    31801 taaaaggtga agtaatcgat cgttttgatg ctggattcaa agccggtgtt gatgcaggtg
    31861 cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata tgctggtgac ttctctgctc
    31921 cagataaagg acgttcaatt gctcaaggta tgtatgcaca aaacgcagat atcatcttcc
    31981 atgcttcagg cggtacaggt aacggtgtat tccaagaagc aaaatcattg aacgaatcag
    32041 gcgacaaaaa agtttgggtt atcggtgttg accgcgacca atcagacgaa ggcgaataca
    32101 cattaaacgg cgaaaagaaa aacttcacat tgacatctac actgaaagca gttggaacag
    32161 tagtagaaga tttggcacaa aaatcagcgg acggtaaatt ccctggcggc gaacacactg
    32221 tttatggatt gaaagaagac ggtgttggtc taacagaagg acaactttct gacgaagcga
    32281 aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa
    32341 ctcctgaaga aaactaaaac catttctata gccttgttag acacatgttt gacaaggcta
    32401 tggtttgatt agaacaagaa ttgttcattt atccaagctg ccttgtgaca gtttggataa
    32461 gggaacaact ccctgaagta atgtttgttt caaaagaaga acgctcacta agcgtgaatc
    32521 ttagtcaatc gttgccctag atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa
    32581 aatattgtca tagaaatgca gaatattacc aaagaatttg gaacattcaa ggcaaatgac
    32641 aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt tgcttggaga aaacggtgct
    32701 ggcaaatcta cattgatgaa cgtgctttcc ggattattag agccgacatc agggaaaatt
    32761 ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca aagccaacca attagggatt
    32821 gggatggtcc atcagcactt tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg
    32881 ttgggaagcg agcctagtcg cgcagggatg cttgaccata aaaaagcacg aaaagagatc
    32941 caaaaagttt ctgaacagta tggattgtca gtcaacccag atgcttacgt tcgtgatatt
    33001 tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac tttaccgagg agcagatgta
    33061 ctgatttttg atgagccgac agctgtattg acccctcaag aaattgatga attaatcgtg
    33121 atcatgaagg aattagtcaa agaaggcaag tcgatcattt tgattacgca taagttagat
    33181 gaaatcaaag cagtagctga ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca
    33241 gtcaacgtta aagacgtaac ctcacaacaa ttagccgata tgatggttgg aagaacggtt
    33301 tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag tcgttttgtc tattgaaaat
    33361 ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa acttgaactt agaggttcgt
    33421 gctggcgaag tacttggtat cgctggaatc gatggaaacg ggcagtcgga gttgatccaa
    33481 gctttgactg gtttgcgaaa ggcagaaagc ggacatatca agctaaaagg ggaagacatc
    33541 actaataaaa aaccccgaaa aatcactgaa catggtgtag gacacgtgcc agaagaccgt
    33601 cataaatacg ggttagtcct agatatgaca ttatctgaaa atatcgcact acaaacctat
    33661 catcaaaaac cttacagtaa aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc
    33721 agagaattga tcgaagaata tgatgttcga acaacgaatg aacttgttcc cgcaaaagct
    33781 ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat
    33841 ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag gtgcgatcga atttattcat
    33901 aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt tactgatcag tttcgaatta
    33961 gaagaaattt taaatgtttc ggatcgtatt gctgttatcc atgaaggaga aattgtcggt
    34021 atcgttgatc cgaaagaaac atctgaaaat gaattaggtc tattgatggc gggctatacc
    34081 ttagaagaag cacgaaaaga gttagaaaag ggggctggcg cacatgaatg atcgcagcga
    34141 aaaattccgt aatttattag tgcctgtact ttcagttgtt ttaggattga tcttaggcgc
    34201 gatattgatg gctgcttttg gttttcagcc catccaaggt tattcttcta tgctagatgc
    34261 agcccttggt tcgcaaagag ggataggtga aacgttaagg caagcaacgc cgttgatttt
    34321 tacagcatta ggtttttctg tagctaactc agctgggttt ttcaatattg ggctttctgg
    34381 acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc
    34441 aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc
    34501 ggctgttcca ggtttacttc gagcattttt tggaacaagt gaagtgatcg taacaatcat
    34561 gatgaactat atcttattat acgtaagcac atttgcttta catgaagtga tgcctgaaag
    34621 ctatcgctca agtttggact cttccaatat gatcagtcaa aatgcctcac tgcggttacc
    34681 atggttaaca caagcttttg gcggctctcg cgtaaactca ggattatttt tagcaatcat
    34741 cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact ttaggatttg aaatccgttc
    34801 tgttggattg aatccttttg cttctgagta tgcaggaatg agtagtaaac gtacgatcgt
    34861 tttgtcgatg gtcatttctg gcgctttggc agggcttggc ggtgtggttg aaggattagg
    34921 aacgtatcaa aacttctttg ttcaaacgac ttcgctatct attggatttg acggaatggc
    34981 tgtttccttg cttggttctg gatcagcaat cggtattctt ttatcagcga tcttgttcag
    35041 tattttgaag atcggtggac ttggcatgca gacaggagca ggtgttccgt ttgagatcgt
    35101 caatgtatcg attgctttga ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt
    35161 catggctaaa gtcttccaag ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa
    35221 accaacgaat caaaaccatg aagggggcga actataatgt cgactatcga attaattgcc
    35281 ttgatcctta cttctacact ggtttattca acaccgctaa ttctaacgtc actaggcgga
    35341 actttttctg aacgaagcgg gatcgtaaac gtagggctag agggaatcat ggtcatgggt
    35401 gcttttagtt ccatcgtttt caacttgacg atgagtccat cacttggttc tatgacacca
    35461 tggatcgcta ttttagtggg tggcgtcgta ggtatcctat tttctctgat tcacgcagta
    35521 gcaacagtca gtttgcgtgc tgaccatatt atcagcggga cagtaattaa tctgatggca
    35581 cctgctttag gtgttttctt gatcaaagca tggtatggta aaggtcaaac tgataacatc
    35641 acgcagaatc tggggtattt ttctttccca ggattagcag atattccggt cattggacca
    35701 attttcttta aaaatacatt cttacctgct tattgtgcta ttttgattgc tgtcctagct
    35761 tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct
    35821 caagctgccg acacattagg tatcaatgta tatgctatgc gttattcagg cgtactgatt
    35881 tctggcttgt taggcggtat gggcggtgct gtttttgctc agtcgatttc cggtaacttt
    35941 tcagctggta cgatcgttgg acaagggttc atctccatgg ctgctatgat ctttggtaag
    36001 tggaatcctt taggggcaat gggtgcctca ttgttctttg gttttgctca aagcttgagt
    36061 atcattggcg cacaattacc agttatttct tcgattccgc cagtattgct gcaaattgct
    36121 ccatatttgc tgacaatcat tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa
    36181 gcaaacggga agaactatat caaatcaaaa taatctttta gcgaataaaa agggtgggtt
    36241 acgggaagtg tttttcgtct cgcccttttt gttttaaagg caataaaaat aaaaagaaag
    36301 aaggaataat catgtttaaa cgcgtacatc tgatcgtcat ggattcagta ggaattggag
    36361 aagcaccaga tgccgataag ttcggtgatg ttggttctga tacattagga cacattgcga
    36421 aagaagcagg attgaacgtt ccacatttag agcagcttgg tttaggaacg atccgtcctc
    36481 ttgaaggtgt ggaatcagtg aaagatcatc aaggctatgc gacgaaatta gaagaagttt
    36541 ctgtagggaa agatacgatg acaggccact gggaaatcat gggattgaat atcaaaactc
    36601 catttcgtgt gtttcctaat ggattccctg atgatctgat caaacaaatc gaagaatttt
    36661 ctggccgtaa agtcgtatgt aacaaacctt atagcggaac agctgttatt gacgattacg
    36721 gtgaacacca aatgaaaaca ggcgatttaa ttgtatatac ttctgccgat cctgtccttc
    36781 agatcgcggc acatgaagaa atcattcctt tagaagaatt atatcggatt tgccaattcg
    36841 ttcgtgatat aacaaaagat gatccgtata tgattggccg aatcattgca cgtccgtacg
    36901 tgggtgaacc aggagacttc acacgaacaa gcaatcgcca tgattatgcg ttgaatccat
    36961 tcggacacac agtccttgat tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa
    37021 agatcaacga tattttcaac ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata
    37081 tggatggcgt tgaccagctg ctgaaagtta tgaaaaacga tttttcagga atcagcttta
    37141 ccaatttagt agattttgat gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc
    37201 atgcgattga agaatttgac ttacgaattc cagaattgct tgaagcgatg gaagaagacg
    37261 acttgctaat gattacagcc gatcacggaa acgatccgac gtttactggt accgatcata
    37321 cgcgagaata tgtaccgtta ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc
    37381 ctcaaggata ttattcagat atttcagcaa cgattgctga gaactttagt gtttcttcga
    37441 ctgaaaatgg cgaaagcttc ttgaaattat taaaataatc tttttaaata ggagaaagca
    37501 aatgaccaag ttaagcgaga tgttacaaga aacgacccag tttatccttg ataaaggagt
    37561 aaaagaaatt gattttggtt tgattctagg ttcaggatta ggtgaattag cagatgaaat
    37621 cgaagatgct gtaatgattt cttatgatga gatcccgttc tttcctgttt cgacagtagt
    37681 tgggcatgca ggaaagctag tttatgggtc tctggcaggc aagaaagtgt tagccatgca
    37741 agggagattc cactactatg aaggtcattc catgcaaaca gtgacctatc cagtacgtgt
    37801 gatggccgga ctaggggccc attcagtcat tgtgactaat gcatgtggtg gtgtcaacga
    37861 atcatttgta ccaggagatt tgatgctgat taccgatcat atcaatttta ctggtgaaaa
    37921 tcctttgatc ggtccgaatg aagatgcaat gggccctaga tttccagata tgagtcaagc
    37981 ttatacgcct gactatcaag caacagcaaa agcagcagca gaaaaactag gtttggatct
    38041 taaagaaggc gtatatatgg gctattctgg accaacttat gaaacgcctg cggagatccg
    38101 aatgtctcgg acaataggag ccgatgcagt agggatgtcg actgttccag aagtcatcgt
    38161 agcagctcac agcggattaa aagtgttagg aatctcttgt attaccaact tagcagcagg
    38221 tatgcaagcg aatctgagcc atgaagaagt tgtagaaaca actcagagag tcaaacaatc
    38281 ttttaaagca ttggttaaag aaacattgac attattgtaa taggaggaac aataaaatga
    38341 gtgtgcatat tgaagcgaaa gaaggcgaga tcgccgataa gattttactt ccgggagatc
    38401 ctttgcgagc taaatatatt gcggagactt ttttggaaga tccagtttgt tacaatcagg
    38461 tacgcggtat gttaggcttt accggcaaat ataaaggaga acgtatttct gttcaaggaa
    38521 caggaatggg aatgccatca gccaccatct atgcccatga attgatccag tcgtatggtg
    38581 taaagaaatt gattcgagta ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg
    38641 atttagtact agcacaaggt gcagcgacca gttcttcgat gatcgaaaaa aatttccaag
    38701 cctttcattt tcctccaatc agtgatttca atctattact gaaagcatat gagatagcta
    38761 aagaaaaggg ctatacggtt catgtaggaa atgttctttc ggaagattct ttctacaaag
    38821 atgatttgac agagacgttc caattagctg aacttggcgt tttaggagtg gaaatggaag
    38881 cagcggctct ttattactta ggcgcgaaat accatgtgca gacattaagt ttgatgaccg
    38941 ttagcgacca tttgatcacc ggtgaagaaa caacagctgc agaacgtcaa tctacgttca
    39001 atgaaatgat cgaagtagga ttggaaacag cgatagcaga ataagaagga aatgaaaaag
    39061 ctgccctgca ttaagtaata agaacatcca agcaaaaata gctcctcatg tttttgactt
    39121 ggctggactt attattgcaa atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat
    39181 ctgccgttta actctgagtc acaaggaaca atttcaaaaa acagcttttc atgtttgatg
    39241 aatattgtga cttgtgaccg agaagttggc aggagaaaac cgtttatacg aaaaaaggga
    39301 ctgtgacaaa tcgtgtcaca gtcccttttt tatagtgttt tttataggta gtatttttta
    39361 gcaactgtta agtcatcatt taattcataa acaagtggtt gacctgttgg aatttcaaga
    39421 tccatgatgt cttcgtcaga gataccttcg atatgtttag ctagggcgcg taatgagtta
    39481 ccatgtgcag ctactaaaac agttttgttg tctagtaatg ctggagcaat ttcatcttgc
    39541 cagaatggca aagcgcgttc taatgtcact ttcaagtttt ctccaccagg aatatcgcgt
    39601 ttgtctaaca ttgcgtaacg acggtcattt gctgcagaac cttcgtcagt ttcttccatt
    39661 aatggaggaa gagtatcgta agaacgacgc cagatgtgca cttgttcgtc gccgtatttt
    39721 tcagctgttt cttttttgtt caaaccttgt aatttaccat agtgacgttc gtttaagcgc
    39781 catgatttga tttgtggtac ccaaagttga tctgaatatt ctaagatcaa gttacatgtt
    39841 ttgattgcac gagttaaaac agaagtgtaa gcaacatcaa attcgattcc agcttctttg
    39901 attttgcgtc cgccttcttt tgcttcttcg attccttctg gtgctagatt tacatcagcc
    39961 caaccagtaa attggttcaa tgcattccat tcactaagac cgtgacgaga aaaaactaat
    40021 tttggcatgt ttagctctcc ttttaagata ggtttatatt tctgaaattt tcagttctgt
    40081 tttattgtac aattttttgg aagagatttc catcgaaaaa ttagataaga ttctatgaga
    40141 aaacaagtag agaatataaa aaaacgaaca gtgcccacgc aactgttcgt ttttaggtat
    40201 tagatgcata attgctcgat ttctttttca ggtaaattga tatatttata acatgttcca
    40261 actgaaacac cgcattttga agaaataaat tggatcgttt cttttttttc ataatagaga
    40321 cgtcggattt ttttgatcgt ttttgcatcg atttttggtc ttccgccgat ttttcctttt
    40381 ttacgagctt catctaatcc aactaatgta cgttctttga tcaatgcgca ctccatgttt
    40441 gctaggtttt ccattaattg gaaatagatt tttcccattt cgcgcgtatc gattccttca
    40501 tcaagactaa tcaagtgcag cccgcgatct ttgaataatt gcgtgagttc tgttaattgc
    40561 cgcgtagatt tgcctaatcg atgaagttgg caaatgacta gtgcatcgcc ttgttgcatg
    40621 tcagcgatca ctgactccaa atgaacagca gtagaatcat tttgttccac ttcatatgtc
    40681 tcatggaaaa tttcttgaca accaaagtca gataattgat caacctgagt tgttaaatca
    40741 acgtcattta tggttgtgcg tgcataacca ataattttca tggtgattcc tttctagcag
    40801 tgattttcta actggatata acagtaaaaa tgtagcataa agtacgtcaa aaagatagtt
    40861 ttttcgcaaa ttttccgctg tcttttttta ttggatgatc aagcagtttt tctttattta
    40921 cgacggattg cgtcaagaat ctcaacgcct ttttctgagc cgacaactac tttagaaacc
    40981 atgtttagga aaagcccatg ttctactacc ccaacaagtt catctaaata ggtagctaag
    41041 ctttctggct gttcgatgac ttctagatgc agatcaatga tgtaatgacc accatctgtg
    41101 gttaagactt ctccgttttc gtctgtacgc aagacgggtt ggaatctttt ttcttcaaaa
    41161 atatgaagta gctgttggga gccataaggg atcacttcaa ctgggagtgg gaacttccca
    41221 agtttatcga ctaatttaga agaatcaacg atccaaatcg tttcttttga gtacgtagcg
    41281 actattttct cgaaaagcaa agcagcgccg ccgcctttga tcccttgaaa atcagcactg
    41341 atttcatcag caccgtctat tgtcaaatcg actacgggga cttcatcaat actctttaac
    41401 ggtattccta atttttccgc ttgttctttt gtcgcatttg aagtagtaac tcctgtgata
    41461 cgtaaacctt cttcacgcat acggcgacca atctcttcta ccatataata ggcggttgaa
    41521 ccggtcccga gacctacgat catcccgtcc tttacgaatt tcgcggcttc aatgccagct
    41581 aattgtttta aattcatctt tgcgtccacc tttttttagt ttgagcatcg tgctttcctc
    41641 actattttag cgtgatttac ggtaaaaaga taccattctt ttttattcca ataaagcttt
    41701 ttcaaaaaaa attgcatctc ttgttgacat gttttttcat tcgtgatatt ctaacaaagg
    41761 tgctttatct acaggtttct gtgaagaaac gtgctgactg tttatcaaag catatttgat
    41821 aaggatgcag acattgcatt tattttttat cacaaaaaaa gaaccacctg gatgtgtgga
    41881 actagacgca aatcgtggga aggaggaaac aaggaatgcc tacaattaac caattagtac
    41941 gtaaacctcg taaatcaaag gtggaaaaat ctaattcacc tgctttgaac aaaggatata
    42001 acagctttaa gaaaactcaa actaacgtaa actcacctca aaaacgtggg gtagcaactc
    42061 gtgtgggtac aatgacacct aaaaaaccga actcagcttt acgtaaatat gcacgtgttc
    42121 gtttgtcaaa cttaattgaa gttactgctt atatcccagg gattggtcat aacttacaag
    42181 aacacagtgt tgtattatta cgcggtggtc gtgtaaaaga cttaccaggg gtacgttacc
    42241 atatcgttcg cggtgcttta gatactgctg gtgttaacga ccgtaaacaa agccgttcta
    42301 aatacggaac taaaaaacct aaagcttaat tttaattatt aaacaaatga aacttgcttc
    42361 tactatatag aagtaattac tatgaacaat atttcggaag gaggagttac ggatgccacg
    42421 taaaggtcct gttgcaaaac gtgatgtttt accagatcct atttataact caaaattagt
    42481 aactcgcttg atcaaccgtg taatggttga cggaaaacgc gggattgcag ctaacattat
    42541 ctataatgcc tttgacttga tcaaagaatc tacaggtaac gatccattag aagtgtttga
    42601 acaagcaatg aaaaacgttc aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc
    42661 aaactaccaa gtaccagttg aagttcgccc tgaacgccgc gttactctag gtcttcgttg
    42721 ggtagtaaac tacgctcgct tgcgcggtga acacacaatg gaagaacgct tagcaaaaga
    42781 aatcatggat gctgcaaaca acacaggtgc ttctgttaaa aaacgtgaag acactcataa
    42841 aatggctgat gccaaccgtg cgtttgctca ctatcgctgg taagattctc tctgtttggt
    42901 cggcttttat aagtcgacct cagatagtgt ccttattttt agcaactaga tagtaaagag
    42961 aggagaaaca aggagaatga ccagagaatt ttctttacaa aacactcgta atatcggtat
    43021 catggcccac atcgatgctg gtaaaacgac tgctactgaa cgtatcttgt actatactgg
    43081 taaaatccat aaaattggtg aaactcatga aggagcttca caaatggact ggatggaaca
    43141 agaacaagaa cgtggtatta cgatcacttc tgctgctact acagcacaat ggaagggtca
    43201 ccgaatcaac atcatcgaca caccaggaca cgtagacttc acagttgaag ttgaacgttc
    43261 attgcgtgta cttgatggtg ctgtaactgt tcttgatgca caatcaggtg ttgaacctca
    43321 aactgaaaca gtttggcgtc aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa
    43381 caaaatggac aagatcggtg ctgacttctt atattcagta tcaactttac atgaccgttt
    43441 gcaagcaaac gctcacccaa tccaattacc aattggtgcg gaagacgact tcacaggaat
    43501 tatcgacctt gtaacaatga aggctgaaat gtatacaaac gatcttggaa cagagatcga
    43561 agaaacagaa attcctgaag aataccgcga attagctgaa gaatggcgcg aaaaattggt
    43621 tgaagctgta gctgaaactg atgaagaact tactttgaaa tacttagaag gtgaagaaat
    43681 cactgaagct gagttaaaag aaggtatccg ccgcgcaaca gttaacgtag aattctaccc
    43741 agtactatgt ggttcagcat ttaagaacaa aggggttcaa ttactattgg acgctgttct
    43801 tgattacttg ccatcaccgt tagatatccc agctatcaaa gggattgacc ctaaaactga
    43861 tgaagaagta gaacgtcctg cagacgacag cgctcctttc tcagctttag catttaaagt
    43921 tatgactgac ccattcgtag gtcgtttaac tttcttccgt gtgtactctg gtgttttaca
    43981 atcaggttca tacgtacaaa acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct
    44041 acagatgcat gccaactctc gttcagaaat ctctgaagtt tacgctggag atatcgctgc
    44101 tgctgttggt ttgaaagaca caactactgg ggacacacta tgtgatgaaa agagtctagt
    44161 tattcttgaa tccatggaat tcccagaacc agttatccaa gtcgctattg aacctaaatc
    44221 aaaagctgac caagataaaa tgggtgtggc tttacaaaaa ctatctgaag aagatcctac
    44281 tttccgtgct gaaacaaacg tggaaacagg cgaaacaatc atcgctggta tgggtgaact
    44341 tcacttagat atcatcgttg accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg
    44401 tgcgcctcaa gtatcttacc gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt
    44461 tgtacgtcag tctggtggta aaggtcaata cggtcacgta tggattgaat ttacaccaaa
    44521 tgaagaaggt gctggtttcg aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga
    44581 atacattcca gctgttgaaa ctggtttgaa agatgctatg gaaaatggtg tcttagctgg
    44641 ctatccatta gtagatatca aagctaaact ttacgatggt tcataccatg atgtcgattc
    44701 aaatgaaact gccttccgtg ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc
    44761 taatccagta atccttgaac caatcatggc tgtagaagtt gttatccctg aagattattt
    44821 aggtgatgtt atgggtcacg taactgctcg tcgtggacgt gttgaaggta tggaagctcg
    44881 cgctggcggc caacaagttg ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc
    44941 tacaactctt cgttctgcta ctcaaggtcg tggtacattt acaatgacat ttgaccacta
    45001 cgaagatgta ccaaaatcag tacaagaaga aatcattaag aaaaacggcg gaaaagctga
    45061 ataatttctt aaaaccggat agtcctgcaa agagattatc caaagagact atatttttaa
    45121 tatagtttcg tgtaaaataa ttatcgatga tatgggcatg tggtgtacct tgtgtacatc
    45181 atatacctat cagacaaata aaaaatatat tgtattttta ggaggacatt ttaaaatggc
    45241 aaaagaaaaa tttgaccgtt ctaaaccaca cgttaacatt ggtactatcg gacacgttga
    45301 ccatggtaaa actacattaa cagctgcaat cacaactgta ctatctaaga aaaacggcgg
    45361 ccaagctatg gcttacgatc aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac
    45421 aatctcaaca gctcacgttg agtatgaaac agatacacgc cactacgctc acgttgactg
    45481 cccaggacac gcggattacg ttaaaaacat gatcactggt gctgctcaaa tggacggagc
    45541 tatcttggta gtttctgctg ctgacggccc aatgcctcaa actcgtgaac acatcctatt
    45601 gtctcgtcaa gttggtgttc cttacatcgt tgtattcttg aacaaagtag acatggttga
    45661 tgacgaagaa ttactagaat tagttgaaat ggaagttcgt gacctattaa cagaatacga
    45721 attccctggt gacgatgttc ctgtagttgc tggatcagct ttgaaagctc tagaaggcga
    45781 cgcttcatac gaagaaaaaa ttcttgaatt aatggctgca gttgacgaat acatcccaac
    45841 tccagaacgt gacaacgaca aaccattcat gatgccagtt gaagacgtgt tctcaattac
    45901 tggacgtggt actgttgcta caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga
    45961 agttgaagtt gttggtattg ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat
    46021 gttccgtaaa ttgttagact acgctgaagc tggagacaac attggtgctt tactacgtgg
    46081 tgttgcacgt gaagacatcc aacgtggaca agttttagct aaaccaggta caatcacacc
    46141 tcatacaaaa ttctctgcag aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac
    46201 tccattcttc actaactacc gtccacaatt ctacttccgt acaactgacg taacaggtgt
    46261 tgttgaatta ccagaaggaa ctgaaatggt tatgcctggc gacaacgtaa caatggaagt
    46321 tgaattaatc cacccaatcg ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg
    46381 acgtacagtt ggtgccggtg tcgttacaga aatcaaagct taattcactt aagttttaaa
    46441 gtaaagcaac atttattcgg acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc
    46501 gagctttttt atttattgca ggatcaggaa aaaagacagg agtattataa atagaagaaa
    46561 cacgaaataa aagagtcagt attttctaaa tgaaacttta tgaaaacaaa atgtttttta
    46621 caagaaaaag ccttgctttg tgagtttata tatattataa tgacaaaggt gcgtttactt
    46681 tagtgaattg cacagacgta atcagaatga tttcgtcggc taagaaacga gaggttgcga
    46741 cacgcccgga cgctttgcca cgaacgagcg tgacggaaat tttcgtggag ctatgtctac
    46801 ttttcaaaat aggcgaagga gggaacaaga tggcaaaaca aaaaattcgt atccgtttaa
    46861 aagcgtatga acaccgtatt ttagatcaat cagcggataa aatcgtggaa actgcaaaaa
    46921 gaactggagc tagcgtgtct ggtccaattc cattaccaac tgaacgcagt ctttacacaa
    46981 tcatccgtgc gactcataaa tacaaagatt cacgcgaaca attcgaaatg cgtacacaca
    47041 aacgtcttat cgacattgtg aacccaacac caaaaactgt tgatgctttg atgaagctag
    47101 acttaccatc tggtgtaaac atcgaaatca aattataaaa ctaaaacaaa aataatggag
    47161 gtgtactcat gaccaaagga atcttaggga aaaaagtggg aatgacacaa atcttcactg
    47221 aatctggtga attaattcca gtaactgttg tagaagctac tccaaacgta gtactacaag
    47281 tgaaaacaat ggaaacggat ggctacgaag ctattcaagt tggttaccaa gatatgcgtg
    47341 aagttttatc aaacaaacct gcgaaaggtc atgttgcaaa agcaaacacg gctcctaagc
    47401 gcttcattcg tgaattcaaa aatgttgagc taggagaata tgaagtagga aaagaaatta
    47461 cagtagatgt tttccaagca ggagacatta ttgatgtaac aggtactacg aaaggtaaag
    47521 gattccaagg ggttatcaaa cgccacggac aaagccgcgg accaatggct cacggttctc
    47581 gttaccatcg tcgtcctggg tcaatgggtc cagttgcacc taaccgtgta tttaaaaaca
    47641 aacgtcttgc tggccgtatg ggtggcaacc gcgtaactat ccaaaacttg gaagttgtac
    47701 gtgtcgatgc agaaagaaat gttatcttga tcaaaggtaa tgttcctgga gctaaaaaat
    47761 ctttaattac tatcaaatca gctgtgaaag ctaaataatt gtaggaagaa aggaggaact
    47821 aaggaatgcc gaatgtagca ttattcaaac aagatggaag ccaaaacggt gaaatcacac
    47881 ttaacgaaga aatcttcgga attgaaccta atgaaagtgt tgtttacgat gcaatcgtaa
    47941 tgcaacgtgc gtcattaaga caaggaacac acgcggttaa aaaccgtagc gctgttcgcg
    48001 gcggcggacg taaaccatgg cgtcaaaaag gaacaggtcg tgcacgtcaa ggatctatcc
    48061 gctcaccaca atggcgtgga ggtggcgtag tctttggacc aacaccacgt tcttacagct
    48121 acaaacttcc taaaaaagtt cgtcgtttag caatgaaatc tgtattgtca gaaaaagttg
    48181 ctgaaaacaa cttggtagca atcgaaggct tgaacttcga cgcaccaaaa acaaaagaat
    48241 tcaaacaagt tcttgctaac ttatctatcg actctaaagt attggttgtt ctagaaaaag
    48301 gtaacgactt tgctgcttta tcagcacgta acttaccaaa cgtttctgta gtaacttctg
    48361 ataatgtgag tgtgctagac gttgtatcaa atacaaaagt gttagcaaca caaactgctc
    48421 ttactcaaat tgaggaggtg cttgcataat gaacttacta gacgtaatca aacgcccagt
    48481 gatcactgaa aaatcaatgc ttgccatgga tgacaagaaa tacacttttg aagtggacac
    48541 tcgcgcaaac aaaactttag taaaacaagc ggtagaagct gcttttgacg ttaaagtcaa
    48601 aaacgtgaac atcgtgaacg tacgtgcgaa attcaaacgt atgggcaaat atgcaggata
    48661 tacaaaaaaa cgtcgcaaag cgatcgttac attgactgaa gattcaaaag aaatccaatt
    48721 attcgaagct gctgaataag cactaataga taactcatta aattaggagg gaaaaacgtg
    48781 gcgattaaaa agtacaaacc tacctcaaat ggtcgtcgta acatgacagg ttctgatttt
    48841 gctgaaatca caacatcaac acctgaaaaa tcattgttac aaccattgaa aaaccatgct
    48901 ggacgtaaca acaacggccg catcactgta cgtcatcaag gtggcggaca caaacgccaa
    48961 tatcgcgtga tcgacttcaa acgtaacaaa gataacgttg ttgcaactgt tcaaacgatc
    49021 gaatatgatc caaaccgttc agctaacatc gcgttagtac actatgaaga tggagtgaaa
    49081 gcttacatct tagcacctaa aggactagaa gtgggtatga aacttgtttc aggtccagaa
    49141 gcagatatca aagtcggaaa tgctcttcca ttagaaaaca ttccagttgg tactgttatc
    49201 cacaacattg aaatgaaacc tggtaaaggt ggacaattga tccgttctgc tggtacaagt
    49261 gctcaagtac ttggtaaaga aggcaaatac gtattgatcc gcttgaactc tggtgaagtt
    49321 cgtatgatct tagctgcatg ccgtgctaca atcggttctg taggtaacga acaacacgaa
    49381 ttaatcaaca tcggtaaagc tggacgctct cgttggatgc gtaaacgccc aactgtacgt
    49441 ggtagcgtaa tgaaccctaa tgatcaccca cacggtggtg gtgaaggtaa agctcctatc
    49501 ggacgtaaag caccagtttc tccttggggt caaccagctc ttggttacaa aactcgtaac
    49561 aaaaaagcta aatcagacaa acttattgtt cgtcgtcgca aaaataaata attactttga
    49621 ccatgtgtcg cacattttga gaggaggttc accatgggtc gtagtttaaa gaaaggacct
    49681 tttgtcgatg agcatttaat gaaaaaagtt gaagctcaag caggggttga aaagaaaaaa
    49741 gttatcaaaa cttggtcacg ccgttcaaca gttttcccaa gttttgttgg atataccatc
    49801 gcagtttatg atggacgtaa acatgtacca gtttacatcc aagaagacat ggtaggacac
    49861 aaattaggtg aatttgcacc tacaagaact tatcgtggac atgctgctga cgataagaaa
    49921 acgaaacgct aatttgaggg gaggataaat ccaaatggca gaacaaatca catcagctaa
    49981 agcaactgca aaaacagttc gcacttcacc tcgaaaagcc cgtttagtaa ttgaccttat
    50041 cagaggtaaa agtgtcgcag atgcaatttc aatcttgaaa ttcacaccaa acaaatctgc
    50101 tggaatcatt gaaaaagtat tgatgtcagc agttgcgaat gcagaaaata actttgactt
    50161 agatgttgaa aacttggtag taagcgaagc ttttgttaac gaaggaccta caatgaaacg
    50221 tttccgtcct cgtgcaaaag gatcagcttc tccaatcaac aaacgtacaa gtcatatcac
    50281 ggtagtcgta tcagaaaaat aaggagggat aatcagtggg tcaaaaagta catccaattg
    50341 gaatgcgtgt aggcatcatc cgcgactggg atgctaaatg gtatgctgaa aaagagtatg
    50401 ctgagttctt acacgaagat ttaagaatcc gtaaatttat ttcaactaaa cttgctgatg
    50461 ctgctgtatc aacagttgaa atcgaacgcg caacaggtcg cgtaaacatt tcaattcaca
    50521 cagctaaacc aggtatggtt attggtaaag gcggttctga agtcgaaagc ctaagaaaag
    50581 aattaaacaa attaactggt aaaagaatcc atatcaatat cgtggaaatc aaaaaaccag
    50641 atctagatgc aaaattagta ggtgaaggaa tcgcacgtca attagaaaac cgtgttgctt
    50701 tccgtcgcgc tcaaaaacaa gccatccaac gctcaatgcg tgctggcgct caagggatca
    50761 aaactcaagt atctggtcgt ctaaacggag cagatatcgc tcgttcagaa ggatactcag
    50821 aaggaactgt tcctcttcat actttacgtg cggacattga ttacgcatgg gaagaagcag
    50881 atacaacata cggaaaacta ggagttaaag tgtggatcta tcgtggagaa attcttccaa
    50941 ctaaaaaaaa cactgagaaa ggagggaaat aatcatgtta gtacctaaac gtgtaaaaca
    51001 ccgtcgtgaa ttccgcggaa aaatgcgcgg ggaagctaaa ggcggaaaag aagtagcatt
    51061 cggtgaatac ggtttgcaag ctgttgattc acattggatc acaaaccgcc aaatcgaagc
    51121 tgctcgtatc gcaatgactc gttacatgaa acgtggtggg aaagtatgga ttaaaatttt
    51181 ccctcacaaa tcttatactg ccaaagcaat tggggtacgt atgggttctg gtaaaggggc
    51241 acctgaagga tgggttgcac cagtaaaacg tggtaaaatc atgtttgaaa tcgcaggcgt
    51301 tcctgaagaa gtagctcgtg aagcgttacg tctagcttct cacaaattac caatgaaaac
    51361 taagatcgta aaacgtgagg aaatgggtgg tgaatcgaat gaaggttaaa gaaatcagag
    51421 aattaaccac tgccgaaatg cttgatcaag aaaaacaatt aaaagaagaa ttgtttaatc
    51481 ttagattcca attagcaaca ggtcaattag aaaacactgc acgtattaaa gaagtacgta
    51541 aatcgattgc acgcatcaaa actgtgttgc gtgaacaagc taagtaatta tggaaggagg
    51601 ccattaagcg tatgactgaa ggaagaaatc aacgcaaagt ttatcaaggt cgcgtggttt
    51661 cagacaaaat ggataaaaca attaccgttg ctgtcgaaac aaagaaaaac caccctatct
    51721 atggcaaacg catgaactat tctaagaaat acaaagtgca tgatgaaaac aacacagcaa
    51781 aagttggcga catcgtgaga attatggaaa ctcgtccatt atcagctaca aaacgtttcc
    51841 gtttgttgga agtagtcgaa gaagcagtta ttatctaata acacgagatt ttcctatata
    51901 gattgaatgc aaaatctgaa aggaggatac acaacgtgat ccaagcagaa agtcgtttaa
    51961 aagtcgctga taactcaggc gcacgtgaaa ttttgacgat caaagtcctt ggtggatctg
    52021 gtcgtaaaac tgctaacatc ggtgatgtga tcgttgctac ggttaaacaa gcaacgccag
    52081 gtggggttgt caaaaaaggt gacgtcgtta aagccgttat cgttcgaaca aaatcaggag
    52141 ctcgtcgtac agacggttca tatattaaat ttgatgaaaa tgctgcagtg attattcgtg
    52201 atgataaaag ccctcgtgga acacgtatct ttgggccagt tgcacgtgaa ctacgtgaaa
    52261 acaacttcat gaagatcgtt tcactagcac cagaagtttt ataatcttga gacacttatc
    52321 aaaggaggtg cgaaacagtt atgtttgtta aaaaaggcga taaagttaaa gttatcactg
    52381 gaaaagacaa aaacaaagaa ggcgttgtac tagcagcgtt cccgaaaaaa gataaagtta
    52441 ttgtcgaagg tgtaaatgtc gttaaaaaac accaaaaacc ttctcaagct gctcctcaag
    52501 gtggaattgt tgagatggaa gcgccaattc atgtttctaa cgtgatggtg gttgactcta
    52561 ctggtgtagc tggccgagtt ggctacaaag aagtagacgg caaaaaagtc cgtgtttcta
    52621 aaaaaaccgg tgaagtttta gataaataaa agtttaagga aggagggtct tactgaatga
    52681 accgcctaaa agaaaaatat cttaaagaag taactccatc attgatggaa aaatttaact
    52741 atagctctgt tatgcaaaca cctaaagttg aaaaaatcgt catcaacatg ggtgttggtg
    52801 atgcggtatc aaacgctaaa aacttagata aagcagttga agaattaact ttgatcactg
    52861 gtcaaaaacc aatgattaca aaagctaaaa aatcaatcgc tggattccgt ttacgtgaag
    52921 gaatgccaat tggtgcaaaa gttacattgc gcggagaaag aatgtacgaa ttcttagaca
    52981 aattggtttc tgtttcacta ccacgtgtac gtgacttcca tggtgtaagt aaaaaagcct
    53041 ttgacggtcg tggaaactat actttaggta ttaaagaaca attgatcttc ccagaagtag
    53101 attatgattt agtagataaa gtacgtggta tggacatcgt tattgtaaca acagcaaact
    53161 ctgatgaaga atctcgtgag ttgttgacac aattaggcat gccattccaa aaataattaa
    53221 aggaggcgaa ctacgtggct aaaaaatcaa tgattgctaa aaacaaacgt cctgcaaaat
    53281 tttcaacaca agcttatact cgttgcgaac gttgcggacg tccacattca gtttatcgta
    53341 agttccatct ttgccgtatt tgcttccgcg aacttgccta taaaggtcaa attcccggcg
    53401 tgaagaaagc tagctggtaa aaagtaaatt cgcataagaa ggaggtaaaa gatcaatggt
    53461 catgacagat ccaattgcag attttctaac tcgtatccgt aacgcaaaca tggttaaaca
    53521 tgagtcttta gaagtacctg catcaaaaat taaacgtgac atcgctgaaa tcttgaaacg
    53581 tgaaggtttc gttcgtgatg ttgaatacat cgaagatgac aaacaaggcg tgatccgtgt
    53641 tttcctaaaa tttggtaaaa acggcgaacg tgttatcaca aacttaaaac gtatctctaa
    53701 accaggtttg cgtgcttatg tcaaagctga cgaagtacca aaagtattga acggtttagg
    53761 tatcgcaatc atttcgactt ctgaaggtgt tgtcactgat aaagaagctc gtgaaaaaaa
    53821 tatcggcggc gaagttatcg cttacgtatg gtaatcaagt agaatctaca aggaggtgtc
    53881 tttaagtgag ccgtatcgga aataaagtcg ttgttatccc tgaaggagta acagttactc
    53941 aagacggaaa caacgttaca gttaaaggac caaaaggtga attgactcgt actttttcag
    54001 cagatatcaa aatgaatatc gaaggaaacg aagtaacttt cactcgtcca aacgacagta
    54061 aagaaatgaa aacaattcat ggaacaactc gtgcgaactt caacaacatg gttgtaggtg
    54121 tcagcgaagg tttccaaaaa gcgttagaac taatcggggt tgggtaccgt gcacaattac
    54181 aaggatcaaa acttgtattg aacgttggtt actcacatcc agtagaaatc acaccaccag
    54241 ctggagtaac tgttgaagta ccagctaaca cacaagtaat tgttaaaggt gcgaacaaag
    54301 aagaagttgg cgaattagct gcaaacattc gtggaactcg tcctccagaa ccttataaag
    54361 gtaaaggtat tcgttatgtt ggcgaatatg tacgccgtaa agaaggtaaa actggtaaat
    54421 aatcgtttag aaaaattgca tagaagccag cagcaggaaa aaggcatttg gctgaagttt
    54481 accttcaaaa aaatacattt ttacttgtgc cgcagcttct atttttcgaa acgtatttac
    54541 tgcttgaaat agtagtacaa taaaaataaa gaggtgacaa ttgtgattac aaaaccagat
    54601 aagaacaaaa cacgtcaaaa gagacatcgt cgtgtacgta acaatatctc tggtactgct
    54661 gagcgcccac gcttgaacgt ttttcgttct aacaaaaaca tctacgctca aattattgat
    54721 gacgtagcgg gtgtaacgct agcaagtgcc tctaccttgg ataaagaaat ttcaggtgga
    54781 acaaaaacag aagcagcaca agctgtcggt aaactagttg ctgaacgtgc agctgaaaaa
    54841 ggcattaaag tagtagtctt tgaccgtggt ggataccttt accatggccg tgtgcaagct
    54901 ttagctgaag ctgctcgcga aaatggacta gaattttaga agaaggagga ataccattca
    54961 tggtttatat cgatccaaaa catttggaat tagaagaccg cgttgttgca attaaccgtg
    55021 taacaaaagt tgttaaaggt ggacgtcgtc tacgttttgc tgctttagtt gttgtcggtg
    55081 acaaaaacgg acacgtaggg tttggtacag gtaaagctca agaagttcct gaagcaatcc
    55141 gtaaagcaat tgaagatgcg aagaaaaact tagttgaagt acctatggtt ggttcaacaa
    55201 tcccacacga agtaatcggt gcttttagtg gtggtagaat cctaatgaaa cctgctgtag
    55261 aaggttctgg ggttgctgct ggtggaccag ttcgtgccgt attagaatta gctggggtag
    55321 cagatatcac ttctaaatct ttaggctcaa atacaccaat caacgttgtt cgcgcaacag
    55381 ttgaaggttt gaaacaattg aaacgtgcag aagaagttgc agaacttcgc ggcaaatcag
    55441 ttgaagaatt aatcggataa ggaggacaac aataatggct gaattaaaag ttactttaaa
    55501 acgcagtatt atcggacgtc ctcaaaacca acgcgataca gttaaagcgt taggtcttac
    55561 aaagattaac agttcagtgg ttaagccttc taatgaagca attaaaggca tgatcaatac
    55621 tgtttctcac ctagtggacg ttgaagaaat ttaatttaga aaaatcataa ctagaacatt
    55681 aaggaggtgc caaacctatg aaacttcatg aattaaaacc tgctgaaggt tcacgccaag
    55741 tacgtaaccg tgttggtcgt ggtacttcat ctggtaacgg aaaaactgcc ggacgcggac
    55801 aaaaaggaca aaaagctcgt tcaggcggcg gtgtgcgttt aggatttgaa gggggacaaa
    55861 caccattgtt ccgtcgttta ccaaaacgag gattcacaaa cgtgaaccgt aaagactatg
    55921 cagtcattaa tcttgatgta ttgaaccgct ttgaagacgg agctgaagta actcctgttg
    55981 ctttagttga agctggaatc gtgaaaaacg aaaaagctgg aatcaaagta ttagctaatg
    56041 gggaactaaa caagaaatta actgtgaaag cagcaaaatt ctctaaagca gctcaagaag
    56101 caattgaagc ggctggtgga tcaatcgagg tgatctaatg tttagactat taaaagatgc
    56161 ttttaaagta aaagacatta gatcgaaaat cctatttact gtatttgtgt tgtttgtatt
    56221 tcgcctaggt gcacatatta ctgtaccagg cgtaaatgct gcttcattgc aaaacctttc
    56281 ttctttgcca tttttcaaca tgttgaactt ggtaagtggt agtgcgatgc aaaacttttc
    56341 cattttctca atgggggttt caccatacat tactgcttct atcgtcattc agcttcttca
    56401 aatggacatc gtgcctcgat ttgttgaatg gtcgaaacaa ggggaagttg gtcgtaaaaa
    56461 actgaatcaa gcgactcgat atctgacatt agtattggca tttatccaat ctgtcggtat
    56521 cactgctgga ttcaatcaat acgctcaatt aggtttcgtg aataatccta acgtagcaac
    56581 ttacgtaacg atcggtttga ttctaacagc cggtacgatg tttgttactt ggctaggaga
    56641 acagatcaca gaaaaaggta tcggaaatgg cgtatcaatg atcatttttg caggtatcat
    56701 ttctagattg cctgaaagta tcagagaaat ttatgaagat tactttataa atattgatcc
    56761 ttcagacata tggaaatctg ctatttttgt ggccatttta ctagtagcgg tcttgttgat
    56821 cgtgacattc gtaacatttt tccaacaagc tgaacgcaag atcccgatcc aatatacgaa
    56881 acgcgtagct ggcgcgccta caagcagcta tctgccgttg aaagtaaatg cagctggagt
    56941 tattccagtt atctttgcta gttcgtttat tgctacgcca aatgctgttt tacaagcatt
    57001 gagcggtaga ttcggttctg aaaactggta caaagtgatg atggagatct ttaactataa
    57061 tacagcacct ggtgctacga tttatacgat tctgatcgtt gccttcactt tcttctacgc
    57121 ctttgttcaa gtaaaccctg agaaattagc ggaaaacttg caaaagcaag gaagttatat
    57181 accaagcgta cgccctggta aggggactga agagtatgtc tcaagattac tgatgcgttt
    57241 aagtacagtt ggtgcagtct tccttggttt agtggcattg cttccaatca ttgctcaaat
    57301 ggtttgggat cttccacaat caatcggatt aggcggtaca agcttactga tcgttatcgg
    57361 tgtagcttta gaaactgcga aacagttaga aggcttaatg ctgaaacgta aatatgttgg
    57421 attcattgag taagaaagcg tgttgaggtt tcccctcaac acacgttctc acacatttat
    57481 taggagggaa aacaatgaac ctcattttaa tggggctgcc aggcgcaggt aaaggaacac
    57541 aagctgaaaa aatcattgat gcttatggta ttccgcacat ctcaacagga gatatgttcc
    57601 gcgcagcgat gcaaaacgaa acagctcttg gcctggaagc aaagtcttat atggataaag
    57661 gtgcattagt tcctgatgaa gtaacaaatg gaatcgtgaa agagcgacta gctgaaccag
    57721 atacagagaa aggcttctta ttagatggct ttccacgtac gctagatcaa gcaaaagctc
    57781 tggacgcaat gctaaaagat ttgaataaaa aaattgatgc tgtcatcgat atccatgttg
    57841 gtgaagaaat cttggtcgaa cgtctagccg gccgatttat ctgcagcaat tgtggagcaa
    57901 cttaccataa aattttcaac cctacaaaag ttgaagacac atgtgatcgt tgcggtgggc
    57961 atgaattcta tcaaagagaa gatgataaac ctgagacggt taaaaatcgt ctggctatca
    58021 acatcaaaaa tagtgaacca atcttggctt attataaaga acaaggtttg ctaaacacta
    58081 ttgatggtgc tcgtgaaatc gatgctgtat ttgcagatgt acaaaaaatc attgaaaaat
    58141 agcacgattg cccgtagccg acttgcatta tgcagttact cttgtcaagt tcaataaatt
    58201 atgatagaat atgtcagtcc gacttctaga gaatcctcta gatgatccat tttgagaatt
    58261 ttgaggtggg aactgcagtt tccgccattt tatcgtgtga aaatgcgaaa caagtacaag
    58321 gaggtactgt gcgtggcaaa agaagatatg attgaagtcg aaggtacagt cgtcgaaact
    58381 ttgccgaatg caatgtttaa agttgaacta gaaaacggac accaagttct tgctactgtt
    58441 tcaggtaaaa ttcgtatgca ttacatccgt atcttacctg gtgacaaagt aacggttgaa
    58501 ttgtctccat acgatcttaa ccgtggccgt attacttatc gctttaaata attgtactcc
    58561 gtaaaactac aggaggtatt atcatgaaag taagaccatc agtaaaacca atgtgcgaac
    58621 attgtaaagt aattcgtcgt aaaggacgcg ttatggtgat ttgcccagca aatccaaaac
    58681 ataaacaacg tcaaggataa ttggaggtgc tataaatatg gctcgtattg caggagtaga
    58741 tattcctcgt gataaacgtg tagtagtttc acttacttat atctatggta ttggtaacac
    58801 tactgctaaa aagatcttag ctgatgctgg tgtatctgaa gatgttcgcg ttcgtgaatt
    58861 aacgaatgaa caaacagatg ctatccgtgc agaagtagat aaattaaaag ttgaagggga
    58921 tcttcgtcgt gaagtgaact tgaacatcaa acgtttgatg gaaatcggtt cttaccgtgg
    58981 aatccgtcat cgccgtggat tgcctgttcg tggacaaaac acgaaaaaca atgcacgtac
    59041 tcgtaaaggc ccagctcgcg ctatcgctgg caagaaaaaa taattcttaa gtgaaggagg
    59101 ttaaaacttc atggcagcaa aaaaagtgaa tcgtaaacgc cgtgtgaaga agaatataga
    59161 agcaggtatt gcacatatcc attctacatt caataacact atcatcatga tcactgatac
    59221 tcacggaaat gcattagcgt ggtcatcagc tggttcatta ggtttcaaag gaagcaaaaa
    59281 atcaactcct tttgccgctc aaatggcagc agaaactgct acaaaagcag caatggaaca
    59341 cggattaaag actgttgaag taacagttaa aggacctggt tctggacgtg aagcagcaat
    59401 tcgttcatta caagctacag gtttagaagt gactgcaatt cgtgacgtga ctccagttcc
    59461 tcataatggt gcccgccctc caaaacgccg tcgtgtttaa tgagtgccgc tcattttgag
    59521 ttttatcagt aacgtcattg aacaagacac ttttcgtttt gaaaggggta aagataagaa
    59581 tgattgagtt cgaaaaacca agaatcgcaa aaattgatga agaaaaagat tatggcaagt
    59641 tcatcgttga acctcttgaa agagggtatg ggactacttt agggaattcc ctacgtcgta
    59701 ttttattatc ttctttgcca ggtgctgcca tcactaatat tcaaatcgat ggtgtactgc
    59761 atgaattctc taccatcaaa ggtgttagag aagacgtcac tcaaatcatc ttgaacatta
    59821 aaggcttagc gctgaaaatg tatggccaag aagaaaaaac ccttgaaatc gatattaccg
    59881 gacctgctac agtaactgct ggcgatatta tcgttgatag tgatgtagaa attcttaaca
    59941 aagatatgta catctgtagt gtctctgaag gagctacgtt ccatgctcgc ttaacagtga
    60001 aacctggacg tggttatgtt caagcagatg aaaataaaaa agaagatatg ccaattggcg
    60061 ttcttccagt cgattcaatc tacacacctg ttcgtcgtgt gaactatcaa gttgagaaca
    60121 cacgtgttgg acaccgtgaa gattttgata aattgacgat ggagatttgg acagatggtt
    60181 caattgaacc gttggaagca atgagtttgt ctgcgaaaat catgactgaa catttggata
    60241 tttttgtgaa cttaactgat gaagcaaaaa atgctgaaat catggtcgaa aaagaagaaa
    60301 cacaaaaaga aaaaatgttg gaaatgacga tcgaagagtt agacttgtct gttcgctctt
    60361 acaattgttt gaaacgtgca ggaatcaata ctgtacaaga acttacaaat aaatctgagc
    60421 ctgaaatgat caaagtccgt aacttaggac gtaaatcatt agaagaagtt aaagcaaaat
    60481 tgcacgactt aggtttaggt ttacgtaaag aagattaaaa ccttttacga aggagggaaa
    60541 ccaacgtgag ttatcgtaaa ctaggacgca catctagcca acgtaaagcg atgttgcgtg
    60601 atttaacaac tgatttatta atcaacgaac gtattgtgac tactgaagct cgtgcgaaag
    60661 aagttcgctc aactgcagaa aaaatgatca ctttaggtaa acgcggagat ttgcatgcac
    60721 gtcgtcaagc tgcggctttc gtacgtaatg aagttgccag cgtgcgtgaa gaaaatgagg
    60781 acatcgtcat tgaatcagct ttacaaaaac tgttcaacga tattgcacct cgttacgcag
    60841 aacgtcaagg cggatacaca cgcattttga agacagaacc aagacgcggt gatggtgcac
    60901 caatggttgt cttagaactt gtctaaaatc acactaataa catcacttta tagatgattc
    60961 ttttagagtg ttatgatgtt ggaagatatt tttccgagtc tagctcagcg ctgctcctca
    61021 agcgaatatc gcttggggag taagcactca tcataaagtg tttttttata ccttcaatta
    61081 cctgtcatga cagatatgtt tgaaggcata aaaaaacatt ttaagcgaga aataattgaa
    61141 aattttgtct ttttttgata gtgttagtag gaactttagg atgaagaagg agtaatcatg
    61201 gagccaatta ttgaattaga aaagataaat tataaatacc agccagatga tcttcgcccc
    61261 gcattgaaag atgtctcctt tacaattgat aaaggtgagt ggatcgctat tattggacac
    61321 aatggttcag gaaaatcgac acttgccaaa acgatcaatg gattgcttct gccagaatca
    61381 gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa atatttggac gatccgacag
    61441 atggttggaa tgatttttca aaatccagac aatcagtttg ttggttcgac agtagaagat
    61501 gacgttgctt ttggtcttga aaatcaaggg attcccagag aagaaatgct agtgcgtgtg
    61561 aaagatgcac ttgagaaagt tagaatggct gagtttgctt cacgcgagcc tgctcgttta
    61621 tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg ttgccttacg acctgatatc
    61681 attattttag atgaagcaac cagtatgcta gatccagaag gtcgtgaaga agtgatctct
    61741 acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta tatctatcac tcatgatatt
    61801 gatgaagcag ctaatgccaa tcggatacta gtgatgagac aaggcgaact tgtccgtgaa
    61861 ggtacaccaa aagaaatttt ttctgctggt cctgaactga tcgacttagg tctagatcta
    61921 ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa
    61981 tatatgacag aagaaaggat ggtggattgg ttatggacat ccgttttgaa caagtagact
    62041 tcacttatca gccgaataca ccatttgaac aaagagcctt atttgacatc aatatgacga
    62101 tcaaagaaaa cagttatact gcattagtag ggcataccgg aagcgggaaa tcaaccttac
    62161 ttcagcattt gaatgcgctt gttaagccaa caagcggtac agtccatatt ggagaacgag
    62221 atattcagcc ggatacggat aataagaatt taaagcccat ccgaaaaaaa gttggcatcg
    62281 tctttcagtt tccagaagca cagctattcg aagaaacggt agcaaaagac attgctttcg
    62341 gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt cctagcaaaa gaaacattag
    62401 aacaagttgg gctggatgaa agctatttgg aacgttcgcc atttgaactt tcaggagggc
    62461 aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag gccggaagta cttgtattgg
    62521 atgaaccaac ggcaggactt gatccccagg gcagaaaaga aatgatggag atgttttggc
    62581 gtcttcataa ggaacagcag atcactattg tattagttac gcatttaatg gatgatgtag
    62641 ccaattttgc agattatgtc tatgtgttgg aaaagggacg aatcgttaat agcggggagc
    62701 cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa acaactaggc gtacctactg
    62761 ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa ctttgctacc ttgcctttga
    62821 cagctgaaga attagcagac gctatcgtcc attatgctgg aggaaatgcc catgatgaat
    62881 aaattgatat ttggtcgtta tatgcctgga gattccttga tccatcgatt ggatccccga
    62941 gctaaattat tagccagttt ttattttatc ggaatcattt ttttagcaaa taattgggaa
    63001 acgtaccttt tgttggcatt tttcacatta ctttctgtct tcttgtcaaa agtagatttg
    63061 aaatttttta tccgaggcat tcgcccattg atctggctaa tcctcttcac tgtagctttg
    63121 caggtcttgt ttacaagtgg cggagaaact tattggagtt ggggtgtttt ccatatcact
    63181 gaatttggtg tgatgaacgg cctctttatt ttctgtcgat tcgtgttgat catttttatg
    63241 tcgactctac tcacgcttac tacaccgcct ttggaattat cggatgcgat tgagtacttg
    63301 cttcgtccat tgaaagccgt acggttccca gtccacgaaa tttcattgat gctgtcgatt
    63361 gctcttcgtt ttgtgcctac attgatggac gaaacagaaa agatcatgaa cgctcagcgc
    63421 gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga aaatgaaagc agtcattcct
    63481 ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg aagatcttgc gactgctatg
    63541 gaagcaagag gctacaaagg tggggaagga agaacgaaat atcgtgtatt gcattggcat
    63601 aatagagaca cgatggttat cattgctttt gtattattaa ctgtgctact gatcttttta
    63661 agaggataaa cgttcagtgg gccgctatct attcttttct tgctgatctg tttgcaagaa
    63721 aggatgcaag cagtgtatga caagaaatat tagggtctgt ggcagactaa ggtcacaggc
    63781 ctctttcttt tatctgaaaa gaaagaacga aaaaatttta tagaaaaatc attattccag
    63841 ataacctttt aggaacaaag gagagaaaaa catggtacgt tacaaagcaa tcattgcgta
    63901 cgacggaaca aattttaatg gattccaaaa acagccaaat gggcgtactg ttcaagaaga
    63961 agtagaaaaa acattacaaa aaatggcaaa tggaaaagaa atcacggtct ttggttcggg
    64021 aaggacagat gccggagtac atgcgatcgg gcaagtaatc cattttgatt atccagagga
    64081 gcgtccgtta gaacgaatgc gtttcgcgct agatacccag tcaccagagg atattgctgt
    64141 aagacaagta gaaattgttt cagaagactt ccacgcaaga tatttggtca aagaaaagac
    64201 gtatcaattt cgagtggata tagggaaacc acgcagtcct tttcgacgcc attatgcaag
    64261 ttattttcct tacccacttg atttggaaaa aattcagcgg gcgcttcctg atttattagg
    64321 gactcacgat ttcacctctt tttgtgcatc aggaagctcg atagaggata aaatacgtac
    64381 gatctacgag gctaagatgg aggtaaatga aacaaaagac gaattgttgt ttacatttag
    64441 gggaaatggc ttcttgtata agatgatacg gatcttagta ggaacgcttt tgaaaattgg
    64501 caatggccga ttgcctgaag atagtatccc agatattatt gcgaaaaaag atcgtaatgc
    64561 agcaggacct acagcacacc ctgaaggact ttatttgtat gaagtgaaat atgaataaga
    64621 ggttatgata gaagtggtca gcttcaagaa ataagaaggt atttacgaaa attacttctc
    64681 aaaattttgc aaaatttcgg cttattttcg aagaagctac ttctggaaca ccgtttaaga
    64741 aagaggtcgt gagcctgtca tttaactctg agtcacaaag agcaatttca aaaaacagct
    64801 tctcatcttt gatgaatatc gtgacttgtg gacgaggcgt tgataagtga atcatctttt
    64861 tgttacctat tttttctgta accaaaaaac gatggaacgt tgttttgatt gcccttggtg
    64921 cggacagtct gaacaggttc catcgtttct ttattttcta cgtcttcttc tgtgtgtgtt
    64981 tcgtggttgt tcttctattg ctggatcaat cgtgccataa aaggggcgat tgcggtcaag
    65041 gtaccagaca taaagaataa acaatgcaat acataggaca aaaaggattg cacctatcgt
    65101 aaatccttca ggcaagtaac tgaaagtgac agtatgttta cctgctggaa cttttatcat
    65161 caagaatgcg tctttgaaat tgctgacctt gactggtttc ccatcaattt tagctttcca
    65221 tcctttgtca taaggaatag ttgtcaccaa catggaatct tcagcggtat ttacagtccc
    65281 agaagctttt ctgccagtgg cttccatttc gacacctttt tctttgatct gattcattgc
    65341 ggcttgatag gctacaacat ctagaccaac gactttaggc tccatgaaac ttatttcttt
    65401 cgtcccataa aaacttgcag taaatgtcac tgttgtctct tgaggataat agccaagatc
    65461 atagtattga ccagaaatgt tgatttgcgt ttttcgcgat gtaccattga cagtgatcgt
    65521 cgcactagaa ctttctaatt gcgcaaaatt ggttgggaac aaacttaagt aagcttgtgt
    65581 atttgcaggc acatggactt cccaagtgag ctcttttggc tggttatctg ctgtttctga
    65641 gtaagtgacg ccagtcgttg tctttgtgat cgtcgtattc aactgattct tcaaagtagg
    65701 tgtatagaat gtaaaatact gttcatttag accagacaat gtattgatca agtttgtctg
    65761 actggtcaaa ttatctgtca ctggctgctc aacatcatta acggacaacg gtgcagtaaa
    65821 tgctagtggc aatgcattga cattctgata cagtgtatag tcgccgcttt gaccagcttg
    65881 gcggaagcca tatttactga acgtgctttc gtttttagaa atgttatatt tgattcctgt
    65941 aaacccatcc atcagcaaag tattattgtt ataacggata ttcaaatttg tccctcttga
    66001 acggaaacct aatttatcca ggtaagagga agaattacga tttcttatag aagaaaacaa
    66061 actgatacca ctgtaaccat agttgatcga atcgtttgca gatactggat ccagattttc
    66121 caagcggtaa aacgagtcgg aatctttttt tgtttcattt acaagagctt tcaagtcagg
    66181 ataaggatca gtatataaac tgcgggaagc ataattccaa tcatcaagaa tgccatggat
    66241 catactgttc gtattgacca gtgcttcagc agacatgatc aataataaca atatactcag
    66301 atgattgaat gaaagttttt tatgctgata aaatcccaca gcaaaagcat aaatccctaa
    66361 gaaaaccact gtcaatacaa aggaagtcat cgtcacatag gtgtagcttc cttcaggttt
    66421 gatgccccat gccaccgcaa agagagcagc tacgaccaaa atgattccta acaacagccc
    66481 accatcgtct tgttctatct tttcccatcc ttttcctgca agcaggagca ctaagaaaga
    66541 gaacagatag gcataacgga ataaaaacat attcggtgca tgcataccat gccagaacaa
    66601 attcaatgga acaagataaa aacttgcgat aagtatggcg aaaagactgc cgtacaaaaa
    66661 tttatttttc aacggtactt tccgactggc aaaataaaac agacagagga ctaatggtag
    66721 cagacctgcg taaacgaacg gaatcgatcc gtattttgtc gtgtcataaa caccaatcat
    66781 atttttcatg attagatcca gataagaagt cgcttctgtt ttcaacgtag tgatctgact
    66841 cagtgcctcg ccatttgacc gaagatccag cactgctggc aatacaatga tcattgaagc
    66901 gccgcctgcc agtaaagaag tgataccata aggaacaatc gtttttttat attctgacca
    66961 gtttgtcaat agacgggcga taaaatataa gaaagaaaat acgccaatca taaaacccat
    67021 ataaaaactg gataaaaaca agagtaaata actgataaat aaaagtttcg gccgtttaag
    67081 atccataaca cgatgaatcc ccaaaatcac taatggaaga taaacaaatg catccagcca
    67141 catgatgatc tccgaatgtg cagtgataaa tgagatcaat gcgtaactga cgctaagtgc
    67201 aacatgcccc cagcgaggaa tcttgaacgt acgtgcaaga aaccaaaaag ccaatccggc
    67261 acagccgacc ttaagcaaag tcagaacata cagtgcatct ggcatcatct ggtttggaaa
    67321 aaagaacacc agtggcgtga agatcccgcc tagatagtaa gaaattaaag ataaataatt
    67381 caatcctaga gaagcattcc atgtataaaa aatactttgc tttccaagaa gaacatttcg
    67441 gaagctggca tgaaaattag aaaactgtga aaaggcatca cttgccaaaa tacttctgct
    67501 gcttccagga tagattccga tacttagata aacaagtgct aaaatcaaaa acggaatcag
    67561 gaaactagct gctgcgtagc gtccgttttc tctaaagaat gactttaaat tttttttcat
    67621 atttattttc caccaaattt ctatgaagtt gtattcagtt tatcatatcc ttgtttttct
    67681 ctgcttaaaa agagtctagt tttcacaaaa aaataaaatt tagaataaat aacttcataa
    67741 tttgttcgta taacaaaaat aaacgcaaaa aaagggtagg aatcaaaagt cccaccctta
    67801 aaaattttta ttaaataaaa aaccgttcac tatctgaacg gtccaagtat cattcaatta
    67861 aacgcgttca acttttcctg atttcaaagc acgagttgac acccaaactt ttttaggttt
    67921 tccgtcgacc ataacgcgaa ctttttgtaa gttaggttta acagtacgtt ttgttgagtt
    67981 catagcgtgt gaacggttgt ttccactttt tgtcttgcga cctgtaaagt aacaaacttt
    68041 tgccatggta atttcctcct ttgccttgat ttggagcaat agaataattc agctcatacc
    68101 agactaacat atcatataga atgggaattt gcaagtacat tttcatagat acaattgaca
    68161 attgagaaga ataattgtaa attaaacaag gttatacctg aaattttgca aggtttttga
    68221 gtactttggg taactgcctt ttatttcaag ttgcgctatg ataaaatatg atagaagaaa
    68281 aatgggagtt ttttacccgt taaggaggct cattcatggc tgtaaaaatt caaacgtcag
    68341 ctggaaccat tgaaattacc aatgatgtaa ttgcaacagt tgttggtggc gccgcgacag
    68401 atgtgttcgg tgttgtagga atggccagca aaaatcaaat taaagataat attactgaga
    68461 ttttgcgtaa agaaaattac tcaagaggag tagttgtccg ccaagaagat aatggtattg
    68521 ctgtagatgt ttatacgatt atcagctatg gtacgaaaat ctctgaagtt tcccgcaacg
    68581 tccaagaaaa agtgaaatac aatctagagg cattattagg cgtaactgcc aattcagtta
    68641 atgtctttgt acaaggtgtt cgtgtactgc cagactaggc aggagactgg ctggtaactc
    68701 agcagtcgat ctgaaggagg attatctagg tgagtgtaac agaaataagt gcaagccagt
    68761 tccaggaaat ggtccaggca ggtgcgaatc gtctgcaagt aaatgcagaa tacgtcaatt
    68821 cattaaatgt attccctgtg ccagatggcg atacgggaac aaatatgaat ctatctatga
    68881 ctagtggagc taaggcagta gtagattcaa cctcagaaaa agtaggagaa ttagcagcaa
    68941 tgctttcaaa aggattgctg atgggtgcgc gcggaaattc cggcgttatt ttgtctcaat
    69001 tgtttcgtgg attttctaag caaattccag atgttgtctc attaaatgct tctgatcttg
    69061 ctgcagcctt cactcatgga gttgaaactg cgtataaagc agtaatgaaa ccagtagaag
    69121 gaacgatttt gacagtagct agagaagcag cgaaagctgg agaaaagaaa gcaaaaagta
    69181 ctgatgatgt cgttgaagta atgacagctg ttgtaaaagg agcaaagcgt gctttagcta
    69241 aaactccaga cttgcttcca gtcctaaaag aagtcggtgt cgtagacagt ggagggcaag
    69301 gactgctgtt tgtttatgag ggctttttaa gtgcattgaa tggagaattc caagcggatg
    69361 aaacatatga accttctcca gcagaaatgg atgatatggt caatgcagaa catcatcgca
    69421 gtgtccaagg tcaaatggcg acagaagata tccattttgg ttactgtaca gaaatcatgg
    69481 taaaaatcgg agaaggacct acagtcgata gtacattcga ttacgaagaa ttccgtaact
    69541 atttagatgg tataggagat tctttacttg tggtcaatga tgacgagatc atcaaagtcc
    69601 acgtgcatac agaaaaccca ggagaagtct tgaattatgg acaaaaattc ggttcattag
    69661 tcaaaatcaa agtcgacaac atgcgcttgc agcatgagac gattttggaa cacgatgaag
    69721 aagtgaacga gtttgcgaaa aaagaaatac cacgtatccc gtatggtatc gtagcgatcg
    69781 ctgctggtaa aggcgtccaa gagctgtttg aaagtttagg cgctaattat gttatcagtg
    69841 gtggacaaac aatgaatcca agtacggaag acattgttaa agcgattcaa gaagtaaacg
    69901 cagataaagt gattgtcttg ccaaacaata agaacatctt catggcagct gatcaagcag
    69961 cagaagtagc agatatccca gtagcagttg ttccttcaag aacgatttcc caaggattga
    70021 cagctatgct ttcattcaat gatcaagctt ctttagaaga aaacaaagaa acgatgacag
    70081 aaatgttgtc tagcgttgtg agcggtcaag tcactcacgc gatcagagat acctcaattg
    70141 acggtgtaga gatcaaagaa ggagactttt tgggcatgat cgatggaaaa atcctgatct
    70201 ctaacccaga tatcacagca acagcgttaa gtacactaga acaaatgatc aatgaggaca
    70261 cagagatcgt aacgatcttg gcaggtgatg aaggaacagt tgaacaagca aatgaatttg
    70321 ctgagaaact aacagaaaaa catcctgatt tggaaatcga gatccaccaa ggagatcagc
    70381 ctgtctatcc ttatctattt tctgctgaat aatttctgtg tcagaatatc tttctaacac
    70441 agtattcatt cataaaaggc cttcggttag tgatcaggta tgatcgctga ccaagtcctt
    70501 tttttgttaa aggagggagg aaaaatttat gcgttcttta gatgatccaa tcactatgct
    70561 ggcaggagtc ggagaaaaaa gagcagctag tcttgccagc ttgggcatcc aaacgattga
    70621 agacttactg acttattatc cttttcgcta cgaagatatt caggaaagaa atctaaacga
    70681 gatccaggat caagaaaagg ttactttgaa aggaatcgta gtctcgcctc ctgtgatgaa
    70741 tcgatttggt tataaaaaaa gccgattgca atttcgtatg atgcaagaac atgatgtgtt
    70801 caacgtttct ttttttaatc aaccttatct aaaagaaaaa gtcattttat cggaggaaat
    70861 cgccgtatac ggtaaatggg atgcaaagcg taaagccttg aacgggatga agattttagg
    70921 ttctcaaaca atggatgact tttcgccgat atatcacgtc aacaaatcaa ttcgtcaaac
    70981 gacattagtt gatctaatca aaaaagggtt tcaagaatac ggcacattga ttgaagaaaa
    71041 tctgccaaac aaccttgtgg agaaatatcg actgcttgac cgagcttctg ctgtaagaag
    71101 tatgcatttt ccaaaagacc acgaagaaaa ccatcaagcc aaacgacgtg tcgtttttga
    71161 agagttcttt ttatttcaaa tgcgtcttca aggattgaaa aaagcagaaa aagcagaaac
    71221 gaatggtttg gagatccttt atgatgtcca aaaattgaaa caatttactc aaaagctacc
    71281 ttttgaactg acaaatgcac aaaaacgtgt aacgaatgaa atctgtcggg atcttcgcag
    71341 tccgcagcat atgcagcgac ttttgcaggg agatgtaggt agcggaaaaa cagtggttgc
    71401 cgcgattgcg ttatacgcta ccgttacagc aggtttccaa ggtgcgttga tggtgcctac
    71461 agaaatcttg gcacaacaac atatggaaag cttgcaacag ctgtttgatc ccaatgaagt
    71521 aaccatcgca ttgattacgg ggtcaacgaa agcaaaagaa cgacgtgagc tccttgaaaa
    71581 actagagcaa ggggaaatta acgtcgtgat cggcacacat gcattgatcc aagatggagt
    71641 cgaatttcag aatttaggtc tagttattac ggatgagcag caccgttttg gcgtgaacca
    71701 gcggaaagta cttcgtgaaa aaggctggcg accagatgtc ttatttatga cagcgactcc
    71761 gattcctaga acattagcta ttacagcata tggtgagatg gatgtatcta tcattgatga
    71821 acttccagct ggacgtatcc cgatcgaaac acgctgggta agaacacctc aattggacag
    71881 tgtactggat tggacgaaaa aagaactggc aagaggacat caaatgtatg tcatctgtcc
    71941 gctgattgaa gaatcagaag cattagatgt aaaaaatgca gtggaaatct atgaaaaact
    72001 tcgtgaacta tttgcaccaa aatatgaagt tggcttgtta catggtaaaa tgaagaatca
    72061 ggaaaaagaa gtaatcatgg aagccttcaa agacaaccag atgcagatat tagtttcgac
    72121 tactgtaatt gaagtagggg taaacgtgcc gaacgcgacc gtcatgctga tcatggatgc
    72181 ggatcgattt ggattggcac agctgcacca acttcgtgga cgtgttggac gcggaagtga
    72241 tgcttcctac tgtatcttag ttgctaatcc taaaaatgaa ctaggtgtgg aacggatgaa
    72301 aatcatgaca gagaccacca atggttttgt actaagcgaa aaagatctgg aattacgtgg
    72361 tcctggtgaa gtatttggct tccgtcaatc tggtctgcct caatttgcga ttgctgactt
    72421 ggtgacggat ggaaatgttt tggaggtagc cagagaagag gctgcagccc tttggcaaat
    72481 aaaggattgg cagatgcttc ctgagtatca gcctttagcg caaagcctcc aaatagaaaa
    72541 tcaggataat cgttatttcg attagcagac tgtgctatac tttatactga tgacggaaaa
    72601 aggagtggaa acaatgaaaa ttgccgtaga tgcaatgggc ggagatcacg cacctcaagc
    72661 aattgtagaa ggcgtggctt tagcgcagca agattttcca gaagtagaat tcttacttta
    72721 cggaaaagaa gcagaaatca agaaatactt aacaaatgaa aaaaacatca cgattatcca
    72781 tacagacgaa aaaatcaata gtgatgatga gccagtaaaa gcaattcgac gaaaaaagac
    72841 tgcatcaatg gtccttgctg cacaagcagt gaaaaacggt gaagcagatg ctgtattttc
    72901 agcagggaat acaggcgcgc tactagcagc agggttgttc atcgtaggac gtatcaaaaa
    72961 tatcgaacgc cctggcttga tgtctacctt gccagtagta gggaaagaag gtgccggttt
    73021 cgatatgctg gatcttggcg ctaacgcaga aaacaaacca gaacatttgt tgcaatacgg
    73081 tattttaggt tctttttatg cacgtaaagt ccgaggtatc ggacgtcctc gtgtcgcact
    73141 cttaaacaac ggaacagaag caacaaaagg tagtgaagtg acgaaaaaag cctatgaact
    73201 ccttcagaat gaaacaagct tgaattttat tggaaacgta gaagctcgtg agctactcaa
    73261 tggtgtggct gatgtagtgg tcacagatgg atttacaggc aacgctgttt tgaaatctat
    73321 tgaaggaaca gcaatgaata tgatgaatct attaaaatct gctattctaa atgaagggat
    73381 caaaggaaaa atgggcgcta tgcttttaaa agacggctta cgttctttga aagcagaaat
    73441 ggattattcc aaacatggcg gtgccgtttt gtttggcttg aaagcaccag tcattaaaac
    73501 tcatggagca acaggccccg atgctgttcg ctatacgatc cgccaaatcc acacaatgct
    73561 tgagacagat gtagttggac aattagtcga acaatttgaa aaaagcgaag aatagtattt
    73621 ggatatctga agcgatccgt tccattgaaa aaaaggtgga cattcctgaa aaatgccagt
    73681 aaaattagcc atggtgtagg taattgacaa gtgtatgatt cttggaggtg aaaaagttga
    73741 ctcgcgaaga agtatttaac aaagtagcga aaattatttc aaatcatttt gagatagata
    73801 ccgataaagt gacggatgaa ttgaacatca aagatgattt aaaagctgat tcaattagta
    73861 ttatggaatt cgttttagag cttgaagatg aattcggtac ggaaatttca gatgaagacg
    73921 ctgaacaaat tgaaacagtc ggtggcgcag t
//
LOCUS       DAYYOA010000003        68392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000003 DAYYOA010000000
VERSION     DAYYOA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.003"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..967
                     /locus_tag="ACHBKA_000175"
     CDS             86..967
                     /locus_tag="ACHBKA_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9443901.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            980..2590
                     /locus_tag="ACHBKA_000176"
     CDS             980..2590
                     /locus_tag="ACHBKA_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9443902.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2667..3326)
                     /locus_tag="ACHBKA_000177"
     CDS             complement(2667..3326)
                     /locus_tag="ACHBKA_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9443903.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3609..4274)
                     /locus_tag="ACHBKA_000178"
     CDS             complement(3609..4274)
                     /locus_tag="ACHBKA_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM9443904.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4421..5026)
                     /gene="dhaL"
                     /locus_tag="ACHBKA_000179"
     CDS             complement(4421..5026)
                     /gene="dhaL"
                     /locus_tag="ACHBKA_000179"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM9443905.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5054..6064)
                     /gene="dhaK"
                     /locus_tag="ACHBKA_000180"
     CDS             complement(5054..6064)
                     /gene="dhaK"
                     /locus_tag="ACHBKA_000180"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM9443906.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6039..6434)
                     /gene="dhaM"
                     /locus_tag="ACHBKA_000181"
     CDS             complement(6039..6434)
                     /gene="dhaM"
                     /locus_tag="ACHBKA_000181"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM9443907.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6588..6908
                     /locus_tag="ACHBKA_000182"
     CDS             6588..6908
                     /locus_tag="ACHBKA_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM9443908.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7020..7877)
                     /locus_tag="ACHBKA_000183"
     CDS             complement(7020..7877)
                     /locus_tag="ACHBKA_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9443909.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7985..9288)
                     /locus_tag="ACHBKA_000184"
                     /pseudo
     CDS             complement(7985..9288)
                     /locus_tag="ACHBKA_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9285..9794)
                     /locus_tag="ACHBKA_000185"
     CDS             complement(9285..9794)
                     /locus_tag="ACHBKA_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443910.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9969..12104)
                     /locus_tag="ACHBKA_000186"
     CDS             complement(9969..12104)
                     /locus_tag="ACHBKA_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM9443911.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12187..13842)
                     /locus_tag="ACHBKA_000187"
     CDS             complement(12187..13842)
                     /locus_tag="ACHBKA_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9443912.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13844..14158)
                     /locus_tag="ACHBKA_000188"
     CDS             complement(13844..14158)
                     /locus_tag="ACHBKA_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9443913.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14328..14588
                     /locus_tag="ACHBKA_000189"
     CDS             14328..14588
                     /locus_tag="ACHBKA_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM9443914.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14602..14916
                     /locus_tag="ACHBKA_000190"
     CDS             14602..14916
                     /locus_tag="ACHBKA_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM9443915.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15646..16200)
                     /locus_tag="ACHBKA_000191"
     CDS             complement(15646..16200)
                     /locus_tag="ACHBKA_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM9443916.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16326..18254)
                     /locus_tag="ACHBKA_000192"
     CDS             complement(16326..18254)
                     /locus_tag="ACHBKA_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9443917.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18560..19276)
                     /locus_tag="ACHBKA_000193"
     CDS             complement(18560..19276)
                     /locus_tag="ACHBKA_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9443918.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19277..21100)
                     /gene="glpO"
                     /locus_tag="ACHBKA_000194"
     CDS             complement(19277..21100)
                     /gene="glpO"
                     /locus_tag="ACHBKA_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM9443919.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21114..22610)
                     /gene="glpK"
                     /locus_tag="ACHBKA_000195"
     CDS             complement(21114..22610)
                     /gene="glpK"
                     /locus_tag="ACHBKA_000195"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM9443920.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22746..24206)
                     /locus_tag="ACHBKA_000196"
     CDS             complement(22746..24206)
                     /locus_tag="ACHBKA_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9443921.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24196..25491)
                     /locus_tag="ACHBKA_000197"
     CDS             complement(24196..25491)
                     /locus_tag="ACHBKA_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9443922.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25823..26500)
                     /locus_tag="ACHBKA_000198"
     CDS             complement(25823..26500)
                     /locus_tag="ACHBKA_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM9443923.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26530..27621)
                     /locus_tag="ACHBKA_000199"
     CDS             complement(26530..27621)
                     /locus_tag="ACHBKA_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM9443924.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27722..28156)
                     /locus_tag="ACHBKA_000200"
     CDS             complement(27722..28156)
                     /locus_tag="ACHBKA_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9443925.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28740..30197)
                     /locus_tag="ACHBKA_000201"
     CDS             complement(28740..30197)
                     /locus_tag="ACHBKA_000201"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9443926.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30194..32089)
                     /locus_tag="ACHBKA_000202"
     CDS             complement(30194..32089)
                     /locus_tag="ACHBKA_000202"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9443927.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32147..33922)
                     /locus_tag="ACHBKA_000203"
     CDS             complement(32147..33922)
                     /locus_tag="ACHBKA_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9443928.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33972..35468)
                     /locus_tag="ACHBKA_000204"
     CDS             complement(33972..35468)
                     /locus_tag="ACHBKA_000204"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9443929.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35662..37284)
                     /locus_tag="ACHBKA_000205"
     CDS             complement(35662..37284)
                     /locus_tag="ACHBKA_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9443930.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37733..39046)
                     /locus_tag="ACHBKA_000206"
     CDS             complement(37733..39046)
                     /locus_tag="ACHBKA_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9443931.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39291..39515)
                     /locus_tag="ACHBKA_000207"
     CDS             complement(39291..39515)
                     /locus_tag="ACHBKA_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443932.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39535..39957)
                     /locus_tag="ACHBKA_000208"
     CDS             complement(39535..39957)
                     /locus_tag="ACHBKA_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9443933.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40101..40811)
                     /locus_tag="ACHBKA_000209"
     CDS             complement(40101..40811)
                     /locus_tag="ACHBKA_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM9443934.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40829..41326)
                     /locus_tag="ACHBKA_000210"
     CDS             complement(40829..41326)
                     /locus_tag="ACHBKA_000210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9443935.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41363..41815)
                     /locus_tag="ACHBKA_000211"
     CDS             complement(41363..41815)
                     /locus_tag="ACHBKA_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9443936.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41950..42555)
                     /locus_tag="ACHBKA_000212"
     CDS             complement(41950..42555)
                     /locus_tag="ACHBKA_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9443937.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(43005..43625)
                     /locus_tag="ACHBKA_000213"
     CDS             complement(43005..43625)
                     /locus_tag="ACHBKA_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9443938.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43627..44496)
                     /locus_tag="ACHBKA_000214"
     CDS             complement(43627..44496)
                     /locus_tag="ACHBKA_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9443939.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44519..44926)
                     /locus_tag="ACHBKA_000215"
     CDS             complement(44519..44926)
                     /locus_tag="ACHBKA_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9443940.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44945..46234)
                     /locus_tag="ACHBKA_000216"
     CDS             complement(44945..46234)
                     /locus_tag="ACHBKA_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9443941.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46261..46563)
                     /locus_tag="ACHBKA_000217"
     CDS             complement(46261..46563)
                     /locus_tag="ACHBKA_000217"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9443942.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46588..48681)
                     /locus_tag="ACHBKA_000218"
     CDS             complement(46588..48681)
                     /locus_tag="ACHBKA_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9443943.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49170..49934)
                     /locus_tag="ACHBKA_000219"
     CDS             complement(49170..49934)
                     /locus_tag="ACHBKA_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM9443944.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50045..50914)
                     /gene="mdcB"
                     /locus_tag="ACHBKA_000220"
     CDS             complement(50045..50914)
                     /gene="mdcB"
                     /locus_tag="ACHBKA_000220"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM9443945.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50939..52015)
                     /locus_tag="ACHBKA_000221"
     CDS             complement(50939..52015)
                     /locus_tag="ACHBKA_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9443946.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(52039..52632)
                     /locus_tag="ACHBKA_000222"
     CDS             complement(52039..52632)
                     /locus_tag="ACHBKA_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM9443947.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52636..54312)
                     /gene="mdcD"
                     /locus_tag="ACHBKA_000223"
     CDS             complement(52636..54312)
                     /gene="mdcD"
                     /locus_tag="ACHBKA_000223"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM9443948.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54302..54601)
                     /locus_tag="ACHBKA_000224"
     CDS             complement(54302..54601)
                     /locus_tag="ACHBKA_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM9443949.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54703..56349)
                     /gene="mdcA"
                     /locus_tag="ACHBKA_000225"
     CDS             complement(54703..56349)
                     /gene="mdcA"
                     /locus_tag="ACHBKA_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM9443950.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56356..57267)
                     /locus_tag="ACHBKA_000226"
     CDS             complement(56356..57267)
                     /locus_tag="ACHBKA_000226"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9443951.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57387..58244
                     /locus_tag="ACHBKA_000227"
     CDS             57387..58244
                     /locus_tag="ACHBKA_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9443952.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58431..58793)
                     /locus_tag="ACHBKA_000228"
     CDS             complement(58431..58793)
                     /locus_tag="ACHBKA_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM9443953.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58982..59164)
                     /locus_tag="ACHBKA_000229"
     CDS             complement(58982..59164)
                     /locus_tag="ACHBKA_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443954.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59139..60578
                     /locus_tag="ACHBKA_000230"
     CDS             59139..60578
                     /locus_tag="ACHBKA_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9443955.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60585..63137)
                     /locus_tag="ACHBKA_000231"
     CDS             complement(60585..63137)
                     /locus_tag="ACHBKA_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM9443956.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63251..64582)
                     /locus_tag="ACHBKA_000232"
     CDS             complement(63251..64582)
                     /locus_tag="ACHBKA_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9443957.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64548..66026)
                     /locus_tag="ACHBKA_000233"
     CDS             complement(64548..66026)
                     /locus_tag="ACHBKA_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9443958.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66331..68166)
                     /gene="tdc"
                     /locus_tag="ACHBKA_000234"
     CDS             complement(66331..68166)
                     /gene="tdc"
                     /locus_tag="ACHBKA_000234"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9443959.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19451 a        14583 c        11703 g        22655 t
ORIGIN      
        1 agttttataa aaaggtataa aaccttttta ttaaactatt taatcttgtt tagtatatat
       61 ttttagaaaa aggagtgggg tttttatggc agaaatcgtc aaagtcacac atttgaaaaa
      121 gagctttggt cgtttcgaag ctttaaaaga tgtctctttc accgtacatt caggtgaagt
      181 cgttggattt atcggaccaa atggttctgg aaaatccacg acgattcgga cacttttagg
      241 gattataaaa aaagacggcg gaacggcaac gatttttggg caagatgtat gggaagatag
      301 tttatctatc catgaacgtc tttcttatgt tccaggcgat atctctttgt ggggaaattt
      361 gactggagga gcgatcatcg atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa
      421 aaaagatgaa ctgattcaac gtttctctct tgatcctaag aaaaaagcaa aaaactattc
      481 aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg gctgttgatt cggatctcta
      541 tttatttgat gagcctactt ctggtttaga tcctttgatg gaaacgattt tccaagaaga
      601 agttgaaaaa ttgaaagaac agggcaaatc catcctactt tcttctcata ttctcagtga
      661 agtcgagcga ctagcggata aagtcgtgat catacgcgaa ggaacagtcg tagaagaagg
      721 aacactcgat gaattgcgtc atttgactcg ttcgaccatc actatagaaa cagaagcaga
      781 gaatagtcac ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc
      841 tcaatttgct gctgataacg aagctttcga tgtgatttta gctgaagcgt ccaaattagg
      901 tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat ttattcatga gacattatga
      961 aggatgagga ggaagcgaaa tgactgataa attttcacgc tggtccaccc ttttttctca
     1021 aaaccttagc cgtgactgga aaaagatcct gatatggcta gtcggtgtca ctgctttttc
     1081 tggtggtttt gtccctgcgt ttgaagaatt gacttccgga caaggattag ctggattata
     1141 cgaaaccttg caaaacccgg caatgattgc aatggtcgga ccaactccag cttcgaatgc
     1201 tgctgagtat actttaggag cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt
     1261 tgctgctgtg atctctgttt tacacgtggt gagccaaacg agaaaaaaag aagaacttgg
     1321 tctgattgaa cttattcgtt cctttcgagt cggacgtcaa gcaaactctt tagctgtcat
     1381 cgctgaaagt attctgatca atttgctttt agcaatatcg atcagctgta cgatgatttt
     1441 tttccaagca gattcgatca ctgcagaagg tgcttggctt tttgggctat ctgtaggtag
     1501 tatggggatt ttggggagtg ccgttgcact gtttgctgca caactgatgc caagttcagc
     1561 cagttcgaat ggtctgtctt tagcgttttt aggtggactt taccttctac gagcaataac
     1621 agatgtagct gattcctccc tttcgatgtg gaatccttta ggctggtctt atttgaccta
     1681 tccatttaca actaacaact ggattccttt gctttatttg tttttgttca gtctaatctt
     1741 gataatcagt tcatttgttt tagaggaaca tcgagatttg aacaccggct atatccccga
     1801 acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt tattattcta
     1861 tctaaataaa gggatgacaa tcagttggct tatcactttt ggtttgatgg gggcggcata
     1921 cggctctatc tacgggaata tgcaacagtt tttagaaagt aacgacttaa tgaaacagat
     1981 gtttatccaa tctggcgtct caattgaaaa aaactttaca agcacgatca tggtcgtctt
     2041 gatttgtcta gctgctattt taccaattgc tagcatcaat cgtttattta cagaagaaac
     2101 ccacggacgt atgaatcagc tgttcgcaac aaaaatttct aggaaccaac tttattggac
     2161 caatatgtta ctagctattc tatttgctat attggcaatc ctgattagtt ctggtgcact
     2221 agggctttct gctttggctt cgatgaagga tgcgaaagac atggatctat tgatgtttct
     2281 taaagccggg ttcaatttcc tgcctgccgt tttagtgttt atcggaatta gtggctttct
     2341 attcggatgg cttcctcgaa tgggtaaact tgcttatatc tacttagctt actcctttgc
     2401 actcaattac tttggcggaa ttttggattt accagaatgg ttttccaaaa cagctgtccc
     2461 tagctgggtt ccacggatgc caaacgattc ttttgatctt tccgtgttcc ttacgttatc
     2521 atgtatcagc cttgcactgt tcgtccttgg ttctattggt tataggcgaa gagacttact
     2581 agaaggataa taaaaaagca tgtacaattt catgctagcc tccaaatctg ggtcaaaaat
     2641 ccaacatttg gaggtcattt ttttatttac aagtcttcta actgctcttt atcaatgatc
     2701 cagacttctc gattctttct ttttacataa ccattctcct ctaataattt aaattttcta
     2761 gataaagttt cgggggtcgt accgataaat gcagcaagtt ctttcatttt cattggcagg
     2821 aaaaagtgat ccttccctgt cgctttagct aagtcaagca gatagacagc gactcgttct
     2881 tctacttttt ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa tttctcaacg
     2941 ttgattgcta aaagtttcag actaagggcg ggataatgca gtaagagttc cgagaaatct
     3001 ttttgtctca atatgcaaat ttccgttttt tctagtgctt cgccaaataa agactcattt
     3061 ggaacgccta gcaactggct ctctccctca tatccgcccg gctctattac tcgtaaaagc
     3121 tgttcctttc cgctagcaga taattgatac actttcatac tgccggaggc cacgatcact
     3181 aattgaggtt ctccattcgg cgcaagaatc tgttctcctt tttccacata gtgatgtttg
     3241 acaagtgcgt gtactttttc ttgatcttgc tgtactaagt gattgaacaa aggaacaaat
     3301 gaaacacata aatgatcatg actcataatc tctcctcctt ctaaacagct atttctaata
     3361 aagaaacata acttccctta ttccaactga taattttctt ttatcatagc ataaacgtca
     3421 agcgaaccag ataccagcaa atagagtgga tttctttttg tcccttgctt tattttcctt
     3481 cttttgagac cttaagtaat tgattcctat caatttttta aatatttact ttttgaataa
     3541 tcaaaaacaa accttcactc gcaagccgag agtaaggttt gtttttcgca ttgcttattt
     3601 cgtgttgtct attgaaattt ttccgcacct agcttttgat atagtggtgc tagttcttgt
     3661 aaggtttcag cggttatttg ctcaacgtgt tcgatttctt gcggatagaa gaatagttta
     3721 cccatttgaa aggttcggtc tctcatcgct tttttcaact cttcttgttt ctttgtatcg
     3781 gacaaatagt cagacaaagc taagtggtcg tcaagttcat gttctggttg cggccatatg
     3841 taataatcct tgatatcgac tgttttgctc cactctttca gtatcgcaag caactggtta
     3901 tacgctgcaa tgctaccata tcgttcttcg cttttataat gttggtacat caaatagatt
     3961 tggtattgtt tttgattcaa caaaactgct agacaggcat tctcgtcttg acgagattcg
     4021 cttcggtacg cgctccaaaa atgactgcgt aaattccagc cattcgtcca gctttcgatt
     4081 ttaggtttgc tcatgccgta catatcaggc aaaagtgcag ctgtctggag ctgaatctct
     4141 ttccattttt gccagtgttc tttatattct ttcttgattt gttcaatctt atctgttgga
     4201 tggctctctt tgagctgttt gaatgcgtag attggtcgat caaacaactg ataatctgat
     4261 tttccaagtt ccattgtttc gcctctttcc gttatctttt gtattctacc tgaattttgc
     4321 gatatggaca aatagaggtt gtgaaaaagc tgttctgcat caagtcataa gaacagccaa
     4381 acaaaaaact gcgatgagca tcaccgcagt tttattgaac ttaaggttga ttgatcacat
     4441 ctaaaagcgt tgaaaataaa atggcactag aagcagctcc tgggtcgata tgtccgattg
     4501 cacgctctcc tagataggaa gctcgtcctt ttttagctac caagactttg gtgtgttctt
     4561 ttgcctggtc aatgatttct tttgttaaat tccctgcctt caatgcctct gtgactggca
     4621 gccaaacatc taacatcgtt ttatcgtcag cttctgcctt tcctcggctt tgaatacccg
     4681 ctgttccctg ctcgatcact tcgcctaatt tttcttgaga atcaatagaa tcgacctctt
     4741 ttgtcgcttt catcatgttc atgaatgcag atccgtataa cggaccagaa gccccaccga
     4801 ctttcgatat catcgccata gagaatactt tgaatgtatc gctgattgtt tcaggatgat
     4861 cggcttgaaa agcttcttca taagctgaaa cccctcttcc catgttattt ccatggtctc
     4921 catcgccaat cggggtatct agatcactga gatattgttt gttctgattg attgcctctg
     4981 caaattgcgt gagccattct tggatttctt gaacggttag tttcatatta tcaccctttt
     5041 atcttgagat tttttaccat gaaatcgtct gtacattgct ttctaaagaa tctttccatt
     5101 gtgtctccgt caagtcgatc agtgttagag aaagaccttg catatctagt gaggtcataa
     5161 aattaccgac ttttcggaaa actacattga cgcctctttc ttccaacaga gctaaaacat
     5221 cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc cattccgttg actagtacac
     5281 ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt agtaacaagt tcctttgcca
     5341 gtactttaga aggctgcatc ttttctcgac gatagcctgg ctcaccgtgg ataccaacac
     5401 catactcgat ctcatcttct cctagttcga atcctggttt ccctacttct ggtacagttg
     5461 ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac gaccttttct cccagttctt
     5521 tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc cacgattttg tgtacaagga
     5581 cagtacctgc aactccgcgt tttccagcag tatacgtact attttcaacg gcgatatcat
     5641 catctacgat cacactttct acttcaatat cgtccatagc agctaattcc tttgccatat
     5701 cgaagttcaa tgcatcccct gtataattct taacgatcag caaaactcct tttccactat
     5761 ccgcttcctt aatagccgtc tgaatctgat ctggagttgg agaagtaaac acatcaccaa
     5821 gcaccgctgc acttaacatc ccttccccta caaacccggc atgggatggt tcatgtccgc
     5881 ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg attcttggct acaacacggg
     5941 aagattctac acgatgaaca agttctggat agctttttac taatccttcc agcatttctt
     6001 cgacaatatt gcttggatca ttcatgattt ttttcaattt attcccatcc tttctttccg
     6061 gcattttgtg cttctgttaa gatttgctga gtatcatctg ttataccggc agtgatggct
     6121 gctgcaaaag ctccttccac taaaggcgca tccaccagat gataacgttg ctgctgctct
     6181 tcttccaaca tgtcttttgc cagttccgag ttcaataccg cgctaccgat atccgcaaaa
     6241 acaaaataac tagctgcatc gcttgattca ttgactgctt ctacgatttt catcgggtct
     6301 gatccgatct cgccatttat tccgccgaga gaaaaaattt gcacctcttc ggatttttgc
     6361 atctgctcaa tcatttcttt aataccatct gttacttttt gactatgaga aataagcaaa
     6421 atactttttt tcacaagaat cttcctttct tttacatagt tgctaacgat tggtttcgct
     6481 ttcattaatg aacaattcaa gtatataata aatttctaat tttgggaaga tatgtgtagt
     6541 gcttcttgaa gtttgttata ctttaattaa aagagaggag tgatcgaatg attacaacaa
     6601 caacgaacag tgtagaggga aaagccgtag tagcttataa agggatcgtt tttggtgagg
     6661 tcattactgg gatccatgca tttaaagact tggaggcggg gttgcgaaat atctttggcg
     6721 gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag agaagaagca cttgccgaaa
     6781 tggccgaacg tgcaaaagca ttaggagctg atgcgatcat tggcgtaaaa atggactatg
     6841 aagtattagg cagcgacaat ggcatgctga tggtcacatg tagtggaaca gctgtctcca
     6901 cgagataagt aggggacatc tgttttaata actaaagaga gatgggacat gtagtggcac
     6961 tcaagaatta gatttttcag tctaacttcg aggtactcta ccttcccatc ctctcttttt
     7021 tatttctttt tgcggatgat tccgtaagct gctgcgacta acacacccgc ccctgctaag
     7081 gaataactga cagaagctgt tttttcattt gtttttggca aagtctgact tgttgatttg
     7141 gatggctctg tcgtttgctg aactgtttct gtcgtgcttc cagaggtcac tgatttttct
     7201 cctgccgaat gcaaaatatc ttttattcgt tggaaactag ctgctaagtc agcttttttt
     7261 gcttcaaaat cattagcaac tgtttgtgct tcgctgatct ttccgtggat tgcttgactg
     7321 gccgctgtga tatccgcttg agtttgactt agctttccag aagctgtttg agcaacctga
     7381 tttaattgct gattgaattc ttttgcttgt tggatcttcc cgtcaagatc tgtttggaca
     7441 gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa gttgtgcacg gtcatttccc
     7501 actttatttt taagatcagc taaatcttga tcgattgctg cttggtcttc tttcaatttg
     7561 ttttttgcag cttctaatgc ttcttcgagt tcttttcttt tattttccaa gtcttgcaaa
     7621 tgatcgattt ggtcagaaga ggtagaacta tttattgatg tatcttctga agatgaaatc
     7681 gtttctcctg aattatctgt cggtacattg gaagtcattt ctgtttccga ggcaccgttt
     7741 tcagcaccag aagagcttat agttgttgaa ggttctgttt gagcaggttc agaagtttcc
     7801 gcaaaagctg tctgtcccaa taagaaaata ctggaaacag caattggcca gattaacggc
     7861 ttcattcttt ttttcatcac ttttcccatc cttactactt tttaactaat ttcttattat
     7921 aagcaaaaat gtttggaaga accataaaca caacaggctg tttgtctaaa aaacggtgca
     7981 tcttttattt ttttgtacgt tccgggcttt tccatgtccc gcttccataa cctaaaatcg
     8041 ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc gttcactatc cagaacaaaa
     8101 gcatatatag tggtgcgaaa agaaggtatt tcaattttct tcgtcgatca tctacaatca
     8161 gcgaagctag caactggaaa aatcctgcga ccatttcaaa gcagatgaac acaaaactga
     8221 atgtaaaagt aatataaatc tgttcgtagt ttccttgata tacataatgc ccgattaatc
     8281 cagcaaaaag aactacggaa atacaaaaga atattgacca gacaatactc aatgtttgat
     8341 caatgaagat gatcgttctt ccaatatgtt taaaaggatg gagcatcact tttttgaaat
     8401 tcgtcagcca gacttctgtt ccaccttttg cccaacgttt tctttgtcga tatagcatct
     8461 tcaacgtcac aggtacttcc atgaaaaaga taatctcagg agcaaaaaca gataaccagt
     8521 catttaactg atgatcccaa gcaatactga tgtcttctgt cgctcgatct tgtctgaatc
     8581 cgcccacatc gatcaaggct tcttttcgat acatcgtatt tgctccactg tacgcataaa
     8641 gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc cacgatactt gagaattcaa
     8701 ccgtttgaga ttttgcaatc aatttggtac gattttggac atccatattt gcagtgaccg
     8761 cagcaatatg tcttgcacca gggcgaatga aataattcac atacctaatc agtgcatctg
     8821 gctccggtac cgtgtctgca tcattgctta aaatcaattt cccttttgca aaggcaagtc
     8881 cgatattaaa cgcgtgggct tttcctttat ttttttcgat ccgaaccaca cgaagattag
     8941 cgtatttttt catcagtcta gctaatattt ctggtgtttg gtccgttgaa ccatcgtctg
     9001 tcactaatac ctcatagttg tgataattca atttcgtcat caaatactcg attgtatctt
     9061 cgatcacaat ttcttcatta tgggctggca ccatgatcgt gatgaacggt tcgacactca
     9121 agggaacatc tacccattct ttttgcttat gtttgtataa aaacacataa caccagacac
     9181 caataaacca tacaaatccg cctaaaatcg gataagaaac gagaaaaaat acataataga
     9241 caaacaagta tctaagaata aattacctgt atgggtgatt gagatcattt cagttccacc
     9301 ccattttctt tatacaaatc tgcaatgaat gtcgtatcta gatttttctc ttcggagaca
     9361 gaataaaaac ggaccgtttc tctctcttct tttgttccaa aacgttccgt atacacttct
     9421 tcaagtaatt cttgtctttt tttcaatttt tcatcttcat attcaaaaga tttatttact
     9481 tttttgtata gatttcgatt gttccaccta gtcaatacaa atgaggtaat caggaaactg
     9541 aaaaaaatca atacaaaaaa gccgattaag aaatacagca ttttcttttc tgataaaaaa
     9601 gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta atgggaaaaa gacccatata
     9661 aaaggaagaa caacgatcat ccatcctaaa agagccacta acgtttgcca aatctttaac
     9721 cagtaattac cttttgaaaa atacttatct ttcaccaaaa ttgatgcaac ttcttctctt
     9781 gttacttttt tcatatcctc acctcacgtt tttttgttac gacgtaggag tttaccatac
     9841 atttcatttc ttttcacata aagtgttcct aaatttttcc taagaaaaag aacaaaacat
     9901 ttctcatcag caaatgaaaa atattttgtt ctttttatca gttgttcttt ttatttttta
     9961 tctcttaatt atgttgctct ttccaatacg acaatgcttt tcattcctct agatatcgcc
    10021 gtgtacattt gtctgcgtct tctttgctca tctccatcgt tttcaaatgg atataagatt
    10081 acatgatcaa attccagtcc ttttgctaga ctcaaaggaa tgatttggca gttttctaat
    10141 ccatatgaat tttttttaat cgattcttgt agcgtttggg tttcctcttc tgtttgggtg
    10201 ataactgccg ctttttctcc ttttagagaa accaaaatct cagctagtat tttcaacaat
    10261 tcttcagagg atcgaaaaga gcggtattcg ggttcttttc cttgcggtcg gatcgatacg
    10321 atgtcgatct tctcttgatc cacagcatag gttttgaaaa gctctgtgat tgctccgcta
    10381 gaacggtaac tatttctaag atcataccga tggatcggca gatggtagtc gtcgaaacaa
    10441 cgcataatat cagcaaaagt agtggaagaa ttaaagattg cctgattctc gtcacctact
    10501 aaagtaaagt gagtttttgg aaataattcg atcaatagac ctaattgtgc cagggtataa
    10561 tcttgtactt catcaatcaa tagatagcga aatgctggta catttacctt ttcaacaagt
    10621 aaatgacgaa tagctaatat aatgactgct tcatcaacag tacgtgtggg ttgccaagca
    10681 taagctgagc catatcgttt ttcataaatt ctttcaaaca actgatgctc atccacccat
    10741 gccatttctt cgatttgtct ggttattttt cgatattttt tttgtaaaag tttccttgca
    10801 taagctacaa tactttgttc cgaatcatca gaaatcaatt taccgaataa ttccaactgc
    10861 ctatcttctg tcaacgagag tacctgattc tgcacagcag gactttttgc ttgtttcaaa
    10921 agatgattct tccattcgct catcagtgcc tttttcgttg cttgaagcct atcgatcaat
    10981 ttaggatgag caggaacttt ctcatatatt ttttcgatcg tttttttaga aaggactgtt
    11041 tttccatttt tagtaagtcc acggataaaa ttcggatgat ctaaaaacaa aggatctgat
    11101 tgttttagat tatcgataaa ctttttgcta cgcaaacgtt ctgtctgctc cgatacattt
    11161 tctcccgtga tacgtttgaa ataagcctct tcaccttcta tttcctctgc tgaaagctgt
    11221 gaaaccagct gtatgatggt tagattcagt ggatttcttt cacctaatga tggtaggaca
    11281 ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa tcagcaagtc atcggatgtc
    11341 atcgtatctc gatataaata gagcagatac gcaatacgct gcatgatagc agaggtttta
    11401 ccacttcctg ctacgccatt taccaagata ttttgacttt tcacatcccg aatgattgtg
    11461 ttttgttccg attggatcgt agcagtgata gccttcattt cattggtctc atcttggcct
    11521 aagacttcta ataatacgtc atcttgaata gctacagacg tatcaaaaaa cttgaccaaa
    11581 cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa ccgtatcgat tttattttga
    11641 cgaaccgtat aagaagatgg accaatcatg ttattgtaaa aaagttcggc aattggtgaa
    11701 cgccaatcat aaacaagatt ttcatctgca tcatctgtga aatttgctgt accgatataa
    11761 aaggattctt ttggctcatt atccagaaag tctacaacga cttttccaaa gtagggactt
    11821 ttcaataaac gttcgatctt tgctagctga ttttctgcgt ttttctgttt gatattcatc
    11881 tgatcgattt ctctattttt catttcaatc atcgaaaaag tatccaggtt atctaagtaa
    11941 ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca acgactggtt tccttctatt
    12001 tttgccgtat ccatcgattc ccgcagttcg ttttttttat caattagctt ttgatacacg
    12061 ttcttcagat atgcttcttc taactgtttt tgtgtgttga ccatacacat tctcctcttt
    12121 ttttaaagtg gtggaaccga ttagtttacc tcttttaaga gtcgaatgca agctttttaa
    12181 cgagtatcat ttgttaggat atatcagttc ttcaggtcgg acagtttgat ataaagaaaa
    12241 tgcaccatca aggtttttta cgtggtagcc agactgcttc aagattcttt ccgctagata
    12301 gctacgtaat ccgctatgac agctgacgat atattcttgt tggctgtcta attcatttaa
    12361 tcggtcccga agttcattca aagggatatg tttggcttcc ttgaatttgc ccttttccaa
    12421 ttcttctgct gttcgcacat ctaataaaat ttttcctttt gctagttctg tcggcaattc
    12481 atgccattgt acggtttcac taagcccttc tgcaatgttc attgctgcat atcctagcat
    12541 gtttactgga tctttggctg agccaaatgg cggtgcatac gtcaattcta attccggtaa
    12601 atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa atatcgatcc gtttatccac
    12661 accttttgcc ccaacacctt gtgcaccata aatctcgcca gtctctggat ggaagatcaa
    12721 cttcagtaaa atatctgttg cttctggata atagcttgca tgatcttttc ctgatacatg
    12781 aatgacttca aaagacagtt cgttttcttt ggctgttctt tcacttaaac cggtggatgc
    12841 ggcagtcatt ccaaacgttc gtataattgc tgttccaata ccgccttgat ttcttcgaga
    12901 aattccggaa atcgtgtccg caacttgtct accttgacga ttggctggtg aagcaagaga
    12961 aataagtgca tcttttccag tgatttgctg ttttacaacg atagcatccc ccaccgcata
    13021 aatatcagga atattcgttt gataacgttc gtctactagg atacccccgc gtatgcctaa
    13081 ttcgattcca gcggtcactg ctagctggtt atcaggacga acaccaatgg ataaaataac
    13141 taaatcagca gggatggtac gcccatcatc cagtcgcaat cgtttcccgt ggtcttcaaa
    13201 tccagcgaca gcatgagaag taatcacttc tactccttga tgagacaatt ctttttctat
    13261 aaaagctgcc atttcttcat ccagtgttgg aagaatatgt ggtgcttgtt cgatcaccat
    13321 gacttctaaa cctcttcttt ttaggttttc tgccatttcc aatccgatga atcctgcacc
    13381 aacgatcacg gcacgctttg gctgtttttc taatgcatgc atcactttat cgatatctgg
    13441 tacatttcta atagaaaaaa cattgtcggc ttctgttatc cctggaatcg atggaacaac
    13501 tggttttgca ccaggagata aaaccaatgc atcgtactgt tcaatgtgtt tttgaccctc
    13561 atgtacgact tctaccgttt tgttttcggg aaagatggct gtcacttcat gatttggacg
    13621 cacatctaaa caaaaacgag cggataatga ttctggcgtt tggacaagaa gattttcccg
    13681 ttcactgatt tctcccgaaa gataataagg caagccgcaa tttgcaaaag aaacatacgg
    13741 tcctttttca aaaacaacga tttctgcatc ttccattaat cggcgaagcc gtgtagcagc
    13801 agacattcca cctgctacac ctccgacaat aacgattttc atcttatttc cctcctactg
    13861 ttcgatcaaa ccattgattc atgccaccac gtacattcac tacatcataa ccagatttct
    13921 ttaattcttt agctgcttgt ttactcctca tccctgattg acaaatcaca tagacggtct
    13981 tttctttgtc tccatcatat ctgtttatac tttgtaatgg aacattttta gctcctttta
    14041 tatgcccgcc ccggtattct gcaggtgttc taacatcaag taacgctgga ttttccaaaa
    14101 gcttttcctt taattctggg actgttatcg attttattga ttgaaatagc aaattcattt
    14161 tctgcacctc atttacattt ttataaatac ctcatggggt atattaaaca ggagctttat
    14221 taatatgtca accagaatga ctcttcattt ttttcactac tttttatatt tgcataaaat
    14281 accgataagg ggtattatct aattataaaa atagaggaga atgtgttatg gagattgata
    14341 ataaaaatat cctgaaccgt ttgaaacgta cagagggaca aattcgtggt atccaaaaaa
    14401 tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca attaagcgct gttcgttcta
    14461 gtattgatcg cgtaatgggc atgatcgttg cagataatct gaaaaattgc tttgagaaac
    14521 ctgatagtaa tccagaagaa caagcggcta aactagaaca agcgatcaag atgattatta
    14581 aaaagtaaag gaatgatttc aatgaaagtc gttttccata ttgatgaatt agaaaaatgg
    14641 tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt ctccagaaac gacgattgtc
    14701 gtatctgtaa atggcatcgc gattaccggt tatctagact ctgccaatgc agaattctta
    14761 gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta tgcgtgctaa tcatatttca
    14821 gaaagttctt tacctgaaca agtaatcgtt gtaccagcag gtgtccttga tttagttgaa
    14881 ctgcaatccc aaggttatgc ttatatcaag ccttagtcag tagaggttgc gacagaagtg
    14941 atcagcttca agaaatttcg gcttattttt cgaagaagct acttctggaa aaccgtgaat
    15001 caaaggttgt gaaaacgctg tcctgcatca agtaataaga acagccaaac aaaaatagct
    15061 tctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
    15121 aaggaagagg ttgtgaaatc agcggtttga cctaagtatt agagaaaaaa acgggaaaat
    15181 agctcctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
    15241 ttaaggaaga gattgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
    15301 atggatttct acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaatac
    15361 cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga aacaaacgga
    15421 aaaatagctg ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac
    15481 accgtttacc aagaggttgt gagcctacca tttaactccg aatcacaaga aataatttca
    15541 aaaacaactt ctcctgtttt tggtttggtt ggcttattat cgagaagtag ctactgggag
    15601 tctatttatc aaaaaaagga atgcgacata ggtcacaccc cttttttatt ttctttgaaa
    15661 catctttcgg tactcgacag catacaagac aaaagattgt aaaagaccgt aagtgatgac
    15721 ggcaaaaatg actctcaccc acagcgttgg taattctggg aaaagcagca aaaggatcat
    15781 gccaacatag atgacagcag tcgctacttt gccataccac ttcgctccac ttaatttgat
    15841 atttttagat agcccgatta atccagctac aaacataaag ccttctttta tcaagaatag
    15901 accaaacaac agccaaagcc acggtcgata ccatgccata gacaaaatca gaaaaagttg
    15961 tgtcaattta tctgcaaacg gatcaagcac cttccctaag tctgttacct gattatatct
    16021 ccgagcaatg accccgtcta gataatcaga caaacctgag acaacaatga ccgctcctgc
    16081 tatataaaat gactgtctca gcacaaaata aagataaatc ggcagcaaaa aaatacggaa
    16141 tatcgataaa agattcggaa tcgtttgtat ttctttacgc cagtctgtgc ttaatttcat
    16201 tttatcgccc tacctcctca gcactctttt ctttttattt tagcttacac ggggaataaa
    16261 accaatgatt tcactgtttt tatttttctt tacagccact gatattatga gctaatttga
    16321 gataatcact tgctgttttc atcgtttact cttctttctt cctgtcgttt gcgcatttct
    16381 tttactagta ctttatgagc tttttttgat cctcgctcta ggtcacgctg ccgtttacgt
    16441 gttttataat cgataaacag actgtctaaa tcgtaacctg tcgggtacag atcttctgct
    16501 gaacgcacca gacgaacacg ctgacgaggc acgtggatta tttttttgtc taaatataca
    16561 tcaatcgtat cttcacctat atcttcataa actaacgctt ctttttggta ttcattgaca
    16621 aatatccgat caccttttgc aaagagctgg cgttcttttt tattctcctt cctatttgta
    16681 actgctgtct taggaagaaa ttcttttttc tgtgtccgat aggctttttc cgccaagtaa
    16741 cgctctgctt gatggatcaa tttcatcgac atatccattt tatgagcaat ccacaaagct
    16801 tggctttctc ctgtttctcc aacccgcaac tgatatttag gacgaagtgc ttcccgatcg
    16861 aaagccatag cagccggcac aaaatcttca tgatctccgg cgaatttctt gatttctcca
    16921 taatgggtcg tggcaatgat caatgcccct tgctcataca tagattccat gattgcgatc
    16981 gctagtgcag ccccttcatt tggctctgtt ccactgccaa tctcatctaa aagtaccaac
    17041 gtattccttt ttatcttttt caaaatagca gcgatgtttt gcatatgacc agaaaatgta
    17101 ctcaacgcat tttctaaatt ttggtggtca ccgacatcga cgaaaatctc atcaaagaca
    17161 gcaagctctg tcccttcttt tgccggaact tgcataccga acatcgccat caaagtcaat
    17221 aaaccgactg tcttcaacac gactgttttg cctccggcat ttgctccggt gataatcaag
    17281 cctcgatagt ctttaccgat ttcaacatcc aatggtaccg catgctcagg taaaaatgga
    17341 tgtctgcctt gcttgataat gatatgttcc gatttattga ttctaggtgt gcttccattg
    17401 atttctcggc taaattttgc ccgagcaaag atgatatcta acaccgtgat cgtttcgatc
    17461 aataaatcga tcacttcttc gttttctgcg atagcaccag tcaatgccgc caggacttga
    17521 tattcttcac tgatttcttc agcttttaac aactgatagt gttcattcaa tttagaaatc
    17581 acagttggtt caataaaaac cgtcgttctt ttatttgatt catcgataat cgttccatct
    17641 attttatttt tatagctggc tttgatagga atcgtatagt attcgccttt ttgaacaatc
    17701 attgcttctt ggatcatttc tttgtttttc ggatgacgca agaatttcag taattttgac
    17761 tggatttctt tttcgattag ttgtaattgt ttgcgtactt tacgcaaatt acgtgacgcg
    17821 tcgtcactga ctttattatt ttttattttc tggtcaatca actcttctac atttatcaaa
    17881 tcaggaagat gtttgctata agcaaacaac aagggtgtct ggtattgatt cttatcaaaa
    17941 aactttgtga tcatccgact gcttctaaga aaatccgcat attcgatcaa atcggttggc
    18001 tgcagaatca accctttttt gacttgttct gtcagtgcat cgatcctcgg caaccccata
    18061 aatggaacat gctgactgct ttctaagatc aagcgtgctt ctcttgtttc ttcttgacga
    18121 tccaagacag tttgtaggtt tgtcgagacc gtcatatcgc tgattcgttt tttgctatag
    18181 tttccaattg ctcttgcttg gatttccttt ttgatccgat caaattgtaa ttgactaatc
    18241 atttcgttgt ccatattctc tccttttctt gaagagatac ctgtcccaaa ataaataagg
    18301 gcctagagat aaataaaagg atagaacaca gcttaaaaaa gtcatgtcct atccttatta
    18361 gaatacaaag atattgtttt cctgacaggt accgtttcat aagcacacca aataaacttt
    18421 gtttattggc tattcgactt actgattaga taccagctac actcacaaaa cataccccat
    18481 tcttctaggt cttatttatt ttccacttaa ctatagcaag acacattttg gttgtcaaac
    18541 tttagtatgt tttatagatt tacaccatta atgaatagac acctaccgcg ataattgctc
    18601 cgatcattgg acctgcgatt ggtacccatg aatatgccca atctgaagtt cctttattag
    18661 ctattggcaa taccgcatgt gctaatcgag gaccaaagtc ccgagcaggg ttgattgcat
    18721 atcctgttgg ccctcctaat gaaagaccga tggctaagat caatccacct acagcgaata
    18781 cgttcgttcc aggtgcaaat tcgttttgtc caaaagctag caacccaaga actaatacaa
    18841 atgtaccaat taattctgtg atcacatttg ctggataatt ccgtatagca ggacctgttg
    18901 caaacgttcc taaaatcgcg ctttcatctt tcgttgcatt ccaatgtggc aaataagaaa
    18961 gccaaacaag aatggcccct gcaaaagctc ctaaaacttg cgcaatgatg aaaggaacga
    19021 caagattcca actaattgcc cctgtcatcg ccatagctag agagactgca ggattcaaat
    19081 gagctgggcc catgaatcca gaaatataga cagccattgt tacagcgagt ccccaaccaa
    19141 atgcgatgac aatccagcca gaagcctgtg ctttactttt gtttaaattc acagccgcac
    19201 acacaccatc tccaagcaaa actaggatca gtgtcccaat aaactctccc atgatttgag
    19261 tcatatcact gttcatttat ttctccccct ttagttcccg cagatctgat tctgcaatca
    19321 atgcttctaa acgtttagtc tgttgcgcct tctcctcttc tgtccattca aaatatccag
    19381 caattgcatc gatcacaggt tgcttgatct catcaagctg atctcgttca aacaacagat
    19441 gatttgtacg acgaatgaga taatcgcctg gggccaaaac catttcttct tctaaaccat
    19501 aacgtaaccg agctgactca gcgagactca aaccaggata aggtgccatt tcttttgcta
    19561 attcaaagat acgtcgggca ttagtcccgt aaaaatcagc gatataagta gcatcttctt
    19621 cctctaaacc ggctgctact ccttcttttg ctaattctgt cactgtttct tctaatttag
    19681 ttggatcgaa gtttccacct gaaatctgat attttttaga gtcgatttct ttcgtctcta
    19741 ttccgtattc ttcttttaat aattggcgta tcaatcgcaa agctccttct gccatcttac
    19801 ggtaatcggt gattttccca ccagataaag tcagcaaacc atctggttct ctttctaaag
    19861 aactacctct ggaaatcgta gaaggtgctt tttcatcacg gctgttttcc aaatggttca
    19921 acacgtcttc tacttcagca cgagaaactt tgttttcctt atattcactt actgtatcga
    19981 caactttatt gaagctcttg tctgaaatcg atccattatc tccaccattg taatcagaac
    20041 cagaattacc aatcaacagt ggacgaagtc ctgcccagct tgcttcgata tcattcaatg
    20101 tgatatttgc ttctggatag cgatgattga tcacatccaa tagataatct acatcttctt
    20161 gtgtgacttt cggatccgta aaatctcctt ggtaatccgt atctgtcgta ccaaagtaag
    20221 tcttgttttc tcttggaata gcaaaaacca tccgcccatc ttgctttcct gtatcgaagt
    20281 atgtgggttg cggtacaggc agttttttcg catctacaac taaatggatc cctttagttg
    20341 gacgcatttt aggagagact ggacgcgtaa aattcaagtt tcgtacttta tctacccaag
    20401 gaccactcgt attgatcact aattttgctt tgatttcgat cacttcatct gtcagcaggt
    20461 cacgagcttt gacgccaacg atttgatcgc catcatataa aaagccaacc gctttcattt
    20521 tacttactag ataagcccca tcttcggcag cctttttgat attatcaatc actaaacggg
    20581 catcattgtt gcggaaatct agatagacac ccgcgccttt tagcccttct tttttcaaaa
    20641 acggttctct ttccaatact tcttctggtg tcagcgtata attctcatat ttggttcctg
    20701 tcacatttgc cagtttgtca taaaggtcca ttgctacttt gaccgagaac atattgaagg
    20761 ttgttgctcc ttcgccctca tagattggca aaagcattgg atctggtttt gggatgtgtg
    20821 gggcaatact ttgtacgact gcacgttcac caactgtgtc agctactact tccacatcaa
    20881 atgtcttcag ataacgaata ccgccatgaa caagtttggt cgagcgagag gaagtccctt
    20941 ctgcaaaatc ctgcatttcg atcaatcctg ttttgattcc tgatgctgct gactggactg
    21001 ccacaccggc accagtgatt ccgccaccga tgatcaacag atccaattct tcttgctgca
    21061 ttttttgaat gctatcttgt cttgtcttgt ttgaaaacat atggatttcc tccttattca
    21121 aatgcttggg tagcttttac tgcttttttc caaccgttgt aaagtttttc tcggcgctct
    21181 tcctccattt gaacttcaaa tagcttgcca gcttcataaa attcgcggat ctcgtctgta
    21241 tctttccaat aaccgacagc taatcctgct aaaaatgctg cccctagcgc cgttgtttcc
    21301 aagttttctg ctcgttggat cggtacattc aagatatctg tttggaattg catcagatat
    21361 tcattgtttg ctgcaccgcc atctactttc aatacaggaa tcttgattcc agtgtcatct
    21421 tgcatcgtat cgatgatatc tctcacttga taagcaatcg actgtaaagt agctttaatg
    21481 atatcttctt ttgtcgtacc acgtgtcaag ccaaacatag cccctcgtgc tgcctgatcc
    21541 caataaggag cacctagccc aacgaatgca ggaacaacat aaacctcatc ttcgcttgta
    21601 gagcgtttcg ctgcgtcttc tgaatcgcta gctttttgca acatttgaag tccatctctc
    21661 agccattgaa tcgaagaacc agccacaaaa atacttcctt ctaaagcata gtaaaccttt
    21721 ccatttatgc cgtaaccaat tgtcgtcaat aaattatttt tggataattg cggtttttca
    21781 ccggtattca tgacaataaa tgaacctgta ccataagtat tcttgaccat gcctggttcg
    21841 aatgccattt gtccgaataa tgcggcttgc tggtctcctg ccatccctga aattggtgct
    21901 tcgcttccat aaaaatgata accttgcgta taaccataaa cttctgagtt agaagtgact
    21961 tttggtaaca tcactcgagg aatatttagt aaatctaaaa tctcctgatc ccaatctagc
    22021 tgatggatat tgaacaacat cgtccgactg gcattcgagt aatcagtaac atgtgcttgt
    22081 ccatcggtca gtttccatac taaccaagaa tcgatcgttc caaacatcaa ttcgcctttt
    22141 tcagctcgtt cttgcgcgcc ttctacatga tcaagaatcc agcgtatctt cgttgctgag
    22201 aaataggcat cgatcactaa ccctgttttt tcgtggaaaa attcttgata gccttcattt
    22261 tttaagttat ctgcaattcc agctgactgt ctggattgcc aaacaactgc atgatagatt
    22321 ggacgtcctg tatctttttc ccatattact gttgtttcac gctggttcgt tatgccaatc
    22381 ccagcaatct gatttggttt gatccctgat tcgatgaacg ctccggcaat aactgactgt
    22441 acagaattcc agatttcatt tgcatcatgt tccacccaac tttcttgcgg gaaatattgc
    22501 gtgaactctt tttgagaact gccgatgtgt cttcctttct tgtcaaagat aattgctcgt
    22561 gagctagttg taccttggtc aatcgccatg atatacgttg attctgtcat tgtgattccc
    22621 tccattatat gcaaacgctt tctttaacta ctcttatcat actactgttt taaaaaaaga
    22681 aactgtcaaa attttattct ttttttcttt ttttgacagt tttttattat gaagctattt
    22741 tttaatcaat tcttttcttt tttcagcgtc tctattcttc ttttcaatcg aataatgtcg
    22801 taaggtgacg caaattctga cagataatat tcacactgtt ttttgtttct ctggcaaaat
    22861 gtgatcacca aatcatattc tttaccttct tcataacttc caatctcaat accgccgatt
    22921 cccctcagtt ctctgatcag atattgttga aaagcaatac gataaatcgg cagactttct
    22981 aaatcgattc caatcactac tggttttgca atataaaaat cgatcatcaa aagaacattg
    23041 gcatactgga tcatcaggta actaagctca ctatcttcag ctcttttcct cggcaattgc
    23101 tgcaccgctg tttcctgtaa tttcgccagt aactgtgtta aggaatgccc gagcattttc
    23161 tgctgctgtt ccaaaagccg atcacgctca tagacttcga tctttccaac aaagaaaaac
    23221 cattcattgt tgatctggga aatttggtag cctactgcct tttcttcttt gattttcaaa
    23281 cgacgaggac gataatgcca taacatcgtt tcccgaatgt aagtatctaa aacagcggtt
    23341 ggtaactttt tgctccgtgt caaatcatac tgataaaata tttcttcgtc tactatcgaa
    23401 aaagaaacaa aaaagctgta aaacatcatt gtttcgtata cattcagttc tgctgctgtt
    23461 cgactaagat gcatcacgat gattggatcg attgcttgat aaagccggtc cgactgatag
    23521 agtaatttct tttctttcaa agttgcgtac gtactttttt cattcaatag tctttttttc
    23581 gtaatcccga gccagcaagc aatctgtgct tctgctgatg ccgtaaacgt tgtttctaat
    23641 acacggtcca atcctagaat gaacggtctt ttttctggtg tattttgcaa aaaaagcggt
    23701 cgctgatctt ccgggatata ttggaatagt tcataataaa aatatcgtat ctgtagctct
    23761 tctccgtata attgaccgtt tttgatttgt aatccaaatt cctctaagag ctggttcaat
    23821 tctttgatct ttcgaaaaac agaagactca ctgatattaa acgctgtcgc taatcgaacg
    23881 atggatactt gctgatgttc taataaatag acgagcatct taaacttcaa gctttcctgc
    23941 aataaaaaca atagaatttt ttccaaactt acatcttcgt ccaacacaag cagcgcctga
    24001 tactcatttc gctgtacttc tacttttttt tccatcattc ggccaatcaa ttcaatatct
    24061 tccaaatatt gatcaaacga ggcttttgac agttggacat gctctctcaa accattgata
    24121 gaggctgttc ccccttgcag aacaaggttt cgcaaaatca ggatctgtaa ctgctctttt
    24181 ctttctaata atgcttcaat cttcataggc agtattcgcc acctttgacc acaccatatc
    24241 tggcacacgg tattctggct ggaaaaatag ctcatgttct tctaactgtt caaacgtcca
    24301 tccttcttct actgcaattg tcagttggtc aagaaggtgg aacatctctt ttttgctttg
    24361 gagctgtgct ccaagaatct ttttttcaga atcaaataga aatttgagtt taactttgct
    24421 ttgatttatt gctgtaagct tgtagtctac gtatcctgtt tcaatttttt ggggataaaa
    24481 aatgctctct tcttctgtca aacctacgct tcctaagaaa tagccaaacc actgattgcc
    24541 gactggacga taggttccct gatcctttgt ctgtgagcca ctaattgtct tgctgacatt
    24601 tcgtgctgta cggacagcgt tatttactag agaaacatac attttttcat ttgttggacg
    24661 aaaagttact tggatcgcat cgccaatcgc ataaacgtct ttttgagacg tttgcaagta
    24721 gtcatctacc cagatcgttc catcatcatt caatgttaat tgttcttccc acatctgatt
    24781 gtcggggcga gaatgattag ccagcaaaac aagatcatag ttctcctttc ttttttccgt
    24841 ctctacaata gcggtttcat tttcggtcaa agacacaact tgttcttcta aaaaaagtga
    24901 aagcccttga tttttcattt cctttactaa cggttctgtc atttcaggat ctagataacg
    24961 aaaaagaatt gactttcggc tttcgtaaag atggatttgt ttttttcttg atgccaaccc
    25021 ttcagctatt tcgattcctg cttgtcctgc accaataatt gcaattttct ttgcattcat
    25081 gatcttttgc tgaagagccg ttacgttttg tatcttttta actgggtgaa taagcgttga
    25141 ttctgcagaa atatttcgaa acttttgatt tgaaccagtc gctagaatca aacgatcaaa
    25201 atccaatttt tttcctcctg ctaaaacgac ttttttttct ttcaatccta ttactgtctt
    25261 ttctgtgtaa acatggattc gatagcggtc agtcagttct tctttcgtga tccaagaaag
    25321 ttgtttttca ctggtaacct ttccttttaa tatcaaattg aacccgctgg gaatccatcc
    25381 taacttcgat tgcttttcaa tcaaatatat ttcacttgtt ggcatcaatt ggcgaagata
    25441 aatagccgca tgaatccctg caaacgaccc gccgacaatt acaatgttca tctgcattcc
    25501 tctttcttat aaggtgttct actgctaact tctcaatcag aaaacacagt attcataact
    25561 cgctttcaac cttatgatac atagcgatgc agcagaatca accctctcca tcataccgtg
    25621 ttctaggtct gcttctcaat aatgtactag tggacctaat tgaagagtat catttctgaa
    25681 gagatgtttc aaaaaatgta ctcttataaa agaaaaaaac tcgcctttta cgtacctctg
    25741 tccaaatagg aaacagaaaa gaacgaaaag acgagtttta gcgtgtgtaa tgtaatagtt
    25801 agaatagaca catcatttat ttttaattct ttaatgcttc aacaactgct tcgcctaatt
    25861 gatgagcaga ttgaggattt tgccctgtaa gaagccgccc atcaatttga accacttctg
    25921 aaaaagctgc tccagactga tagtttgctc ctttttcttt caaagcatct tctgctaaga
    25981 atggtacttc attcgttgta ccatttgctt cctcttcttc attactgaac ccagtcactg
    26041 tacgtccagc taaaatagat tttccagact catctgtaac tggcaacaat cctacaacac
    26101 cgtgacaaac agctgagacc agcccttcat ttcgatagat ttgtgaagcg actttcccaa
    26161 gatctattgc ttctggaaaa tcccacattg ttccgtggcc accagcataa taaaccgctg
    26221 cataatcatt tgcatccaca tcatcaggac gtaaggtatt tcccaaggcc cgatttctga
    26281 aatcttcatc ttcataatag gcccaatcga tttcatccat attcaaaaca ctttgcggtt
    26341 ctaaaggtac atatccgcct ttaggactga caaaatctgc atcgatccca ttttttacta
    26401 atacatcata aaaatgagtc agttcactta accacaaacc tgtaggacgc tcgatctttt
    26461 cgtacttttc tacatttgtt aatacgatca atactttcat tttcccatcc tctttctata
    26521 aaaattgcgt tattgataac gtgctttcaa gaaattatat aatgggcttg gcaaataaag
    26581 ctctttatat gcttcttcaa aaggtttcgt tacaaattca tcctcacggt tttccaataa
    26641 tttctctgcc cagtcaggat cgacgatcat ttgctgtcca attgctgcaa gttctgcata
    26701 tttcaatacc tgctctacat cttcgcgatt gcggatccca ccgacaccaa ctaaagggat
    26761 ctttccttga atcgtgttat ggaccacttc aagaatcgat ttatcgttgt atttatcaga
    26821 acgagctatt cgatcataca tatttaatga aacatgcaga taatctagtt ttgactcacg
    26881 taatcgttca agaagccaga tcgtgtcttc aaaacggata cctggtgttt catattcttc
    26941 tggtgagaaa cgatacccta cgatgaatgg ttttttagca tattgttcaa ccgctgagaa
    27001 aaccgttgtt aataattctt caataaaacg ataacgattt tccaagctac cgccccattg
    27061 atcctctcgt ctattcgaat gcggagagaa aaattgttgg atcaaatacg tatttgctcc
    27121 atgcaactcg attccatcaa atcctgattc aatcgctcta cgtgtggctt tgccaaatgc
    27181 ttggattgtt tcaacgattt cttcttcact cattgctcgt ggtgtttccg catcaggacg
    27241 ttttgctgca actgcacttg cagatacagg ctgttctcca gataaagttg ctcgacttgt
    27301 cattcgtcca ccgtggaaaa tctgaataat cgcttttgcg ccttctcctt ggattgcttt
    27361 cgctagctca cttaatcgag gaatcatttc atcatgagcg atgctgagtt cacctggcca
    27421 gccttttccg atatcttgga catttgcagc accagtaata acagcaccaa gacctttaga
    27481 acgttgttta taataagtga tctcatcttc ggtgatcact ccattgaaaa agctgagttg
    27541 tgtcgtcata ggcgacagca tcaatcggtt cttcaattga actcctgaag gtaaagtaat
    27601 tgattgatca aattgtgaca ttgtcattcc tcccataatt cgctctgatt aacctatcga
    27661 gaatcatcta tcattaaacc actcgattag ttgtacatgc aactaatttg cttaaaaaat
    27721 tttatttttt ttgtaactta tctgttgcat cattcattgt ctgggctaat tctttacttg
    27781 cttcatttcc caaaactgct ttgtatgact gatgaagttc ttcccagcta ccatcgactt
    27841 ccttagcaaa ttggcgtcct tgttctgtaa gataaactaa tgacaagcga ccttcgctaa
    27901 ctgttttgac gtatccttta tattctaatt tttcaacaaa acgagcaatc gttgatgggg
    27961 cggaatagag catttgtgca atatcttttt gtggtattcc atctttttct tttaatatca
    28021 ataaaataaa accataagtt ggtgccagac ctgtccttct aaacgcttca tcagctaatt
    28081 tattcaacat gcgatctagc tttttcacgc taaagaaaag acattcatct aacgcttttt
    28141 caattggctt ttccatttca tcccctccat tggcgtacat atcatagtat gatttttatt
    28201 ttttgtcaaa aaacacggaa taatgtccgc ctcctctgtc tagatttgaa aaaaagaagt
    28261 agccatcttc aaagaatttc ggcttgtttt cgacaaaact ccttctggaa caccgtgaat
    28321 cagaggttgt gaaaaagtcg tttagttttg agtatgaaga aaaattttgt gaaaatggct
    28381 ttccacattt ttgcataaat ttggcttaat accgaaaaac tggcttttga acaccgttta
    28441 tatcagaggt tgtgaaaaag ccgtcctgca tcaagtaata agaacagcca aacaaaaata
    28501 gttcctcata ttttgtcgtt ttttgagcta atgccgatgg tcagctgatt gtacaccgtt
    28561 tattgagagg tcgtgagcct gccgttgaac tccgagtcac aaggagcaat ttcaaaaaac
    28621 agcttctcat gttttatgac tatggtgact tgtgatcgag gagttggcag gagaatactg
    28681 tttattcgta aagaaggggc tgtgacaaga cttttgtcac agtcccttat ttccttttat
    28741 tataggattt cgccatttga ttggatgact tgtctgtacc aatcaaatga ttttttcttt
    28801 aaccgtttct ttgtgccatt cccttcgtca tcttgatcaa catagatata tccataacgt
    28861 tttgacattt gtgaagtaga agcgctgacg atatcaatgc agccccacga cgtataacca
    28921 aataaatcta ctccatcttt gatagcttcc ttcatctgct caatatgctg tttcaaatag
    28981 tcaatgcggt aatcgtcttg aacggttcca tcttcttcta ctttgtctat agctccgagt
    29041 ccgttttcag ctatgaacag tggtttttga tacctatcat ataattgatt caaagcaatt
    29101 ctcaaaccct taggatcaat ctgccagccc cattcacttg atggaagata cggattttta
    29161 attcctccag ttattaagtt tccttggacc ccttcccaat tttctggatg agcactgact
    29221 gcactagaca tgtagtagct aaaagaaaca aaatcaacag ggtaggctgc taagatttct
    29281 tcatcaccta cttctatttt taagttgaca cctttttctt caaacattcg ttgcgtatac
    29341 gctggataat atcctcttgc ttgaacatca ctaaagaaca aagaagttct ttgctgctca
    29401 attgtttttt gaatatcatc cggatcacaa gaatacggaa caagaagctg atagctgatc
    29461 atacaaccga cttgtgaacc tttgataatt tcatgacaag cttttgtagc taaagcactt
    29521 gctataaact gatggtgtgc accttggtat ttcgcttgtt ccaaatgggg atggttttct
    29581 tcaattactc ctacgctaac aaaagggtga tgttttacac agttgatctc attgaaagtc
    29641 atccaatatt ttactttatt tttatagcgt ttgaatactg tttcagcgaa acgaacgtaa
    29701 aactgaataa gctttcgatt aggccatcca ccatattctg taaccaaata taacggcatt
    29761 tcataatggg aaagagaaac gactggctca atcccatatt ttagacattc gtcaaacact
    29821 tgatcataaa aaaataatcc cgcttcattg ggtgtttctt catcaccatg aggaaaaatt
    29881 cttgtccatg cgatagacat ccgatagcat ttaaaaccca tttcagcaaa taaacggatg
    29941 tcttctttaa agtgatgata gaaatcattt ccgtgtcttt taggataata atacttgctg
    30001 ttgggatcct gcgccgcttt tacttgctga tgcgtcatca tatgccattg atctgtccat
    30061 ttttctttag gtaaatcgct gtcgaatgta tacacatcag acacagataa tcctttttca
    30121 tctacatccc atgcaccttc acattgattg gccgctgtcg ctccgcccca taaaaatcct
    30181 tctggaaatt ttttcatatc gatcccccta aacttgctag ttttcttgtt tgattatttt
    30241 tcttatgact agtaatgata tttttgaatc tactactatt tgtaacaata accattgtgg
    30301 tcggatcata tccctctttg attaattctt gcaaatctac ttccatcaat aaatcgcctt
    30361 tacgtacttt ttttccatca gatactgcac tttgaaagta tttgccattt aaattcactg
    30421 tatcaatacc gatatggatc agtatctctg ctccttcttc actgactacc ccaattgcat
    30481 gtttcgttgg aaaaactgtt gaaacttccc cgtttacagg actgtagatt tttccgtccg
    30541 ttggaatgat cgcaaaccct tctcctaaag atccactagc aaatgttgga tcgtttactt
    30601 cagataaatc aattatttca cctgctgcag gagtacttaa cactatatcc tttgaggatt
    30661 cttcttcgat ttcatccttg tagccgaaaa gaaaagttag catacaggaa ctgatcatcg
    30721 ctaagaaata actagcaact agccaaacaa atccttcacc aatataaatc gggaaagaca
    30781 aaatacttct tgtagcaact gccaaagcac cggctcctgc ggcaccagct actccaccaa
    30841 atatagctcc acatatacaa gcaatgtaaa atggtcgttt atatttaaga gtcacaccat
    30901 aaacagcagg ttcagtgatt ccaaataatg ctgtgataaa tgcagataaa gataaacttt
    30961 ttactttctt atttttcgtt ttcaaaaata ccccaagtac tgctcctgct tgcgcagcta
    31021 cggcagtcat acatgctgga cctagcgtat cacgaccgta gaaagaaaga ttgttggtca
    31081 ttactggtac aagtccccaa tgtaacccaa aaatcactaa cacttgccaa aatccgccga
    31141 taacagcacc agataaaaca gggctaaagt tataaacagc taaatatcca gctgcaatca
    31201 gctgacttgc ataatctgca attggaccta caactaataa agtaattggg accatcacga
    31261 tgatacaaat gaatggtgtc aataaatttc gaatactttc gtgcaagctt ttgcgaatct
    31321 taggctcaat ataagaaaga acccatacag ctaaaattgc tggaataacc gtacttgtat
    31381 accttgctag aatcacgggc atccctagaa atcgcaaggt tatgctatca ctatatgcac
    31441 tgacaatcgt aggataaacg agagctgctg caataaccat agcgatgaaa cgatctgtat
    31501 tgaacttttt tgcagcagta tatgccaaaa atataggcaa gaaatagaaa aatgcatcag
    31561 ctgctgcatt caaaatgata taagttcctg attcagtagt taataaattc aaactcgtac
    31621 ataaggcgag tactcctttg atcaaaccac ttcctgccaa gacgcctaaa gtcggggcaa
    31681 aaacagcggc gatcatgtct ataaaagagt ttaaaatgcc ttttttctca gttcctctct
    31741 ttccactaga tgcatcatct tctagtttca tttgtttgga taatacgtca tacacttcat
    31801 ttactgcatt tcctactacg acttgaaaca ttcctccagc ttcgactaca gtgatcacac
    31861 cttctagttg ctctaccttc tccttatctg ccttcttatt atcttttagg gtaaagcgta
    31921 accttgttgc acaatggtaa acattgctga cattttcttc gcctccgaca aatcggacaa
    31981 tatctttcgc taattgtgaa tagtccattt ttacttcccc cttgttaatt aatgccgtag
    32041 caatccttct tacaagataa gtatagttct tagaaaacgg tttctccatt caattagtac
    32101 cccttataat agcgaacgaa actgtacaat agtgtgtatg gtttacttag ccgagcaaat
    32161 cactcactgc ttgaaagaaa acaagtatat cgtttctctt atagtcaaag gagacaaaaa
    32221 aaacacgctt gatcttcctt tgctgatgta taagtggtat ctcaagctga tactctgtga
    32281 tttttgtttc tacttggctt ttgataagac agcacaacaa agtctcggca tttaacggaa
    32341 gcataactag gtcagaacta ggagagatta gttgctgaat ggaatctatc tttttatgga
    32401 tttttatttg attttcttct ttgtagtaat gatcaagcag ttttgttttc tttttttctt
    32461 caactatggt cagcttttca cgcaaaatct gataaatctc ccaagattca aactcatcta
    32521 gaacactagg tagataaatc aaatcatccc actcaaaaat aagctcttgc tcagtagtca
    32581 aaattgcatc atattccgtt attttgtttg attgattttt gaaaagatac actggttcta
    32641 tccggcattc tttgatccac atgttcatac tttgttgaat ctgttttttt agtgtattca
    32701 aaatactaat tggctggtta ctaacaatca gtatctcccc ttgttttcga aaaggattca
    32761 acgcttcggc aagatacaaa acaaaataag cgctttcagc taaattggga cgtaattgaa
    32821 aatgtattgg gaagaaacgc cgaattaaat aaatttctaa aggataacgg gagatgatct
    32881 tttgtacaaa atgattcatg gtattatgtc cggcatccat cctcgttttc atttgtttga
    32941 tatgggttag caaaaattct gtttcatgga acaataacga aacaggaaga tctaaaaaac
    33001 gaataatcgt ttgctcaaat gctcttagat tcttctctat ttctttgttt ttttcatcat
    33061 taatcatcaa ataatttaac tctaatagtc gattttcaat tgcttgtttt tcttgattgg
    33121 aaaaagaata gggtagttct gatgataact tttttgtgag ggtctctacc atcggaataa
    33181 ataatgtata cctgtttgtt ttttctaaaa gttcttctct tcgaagaatg gtcaaaacga
    33241 gataccgaga aaatcgtatg aagtcctctc ctgaaacaat ataattatat gatataagaa
    33301 cctcttttaa tgtcttttgg acagtcggca tgatattgga aaaaattgta ctgatttgtt
    33361 gaggcaacct tgcgtgttgg atcaccgttt ctgttccaac aagagataag aaaattcttt
    33421 ttgaattttc ttcagcaatg atttttagtc cttttgtcga cgaaacttcc agttctaatt
    33481 taggattgcg ctcgatccaa cgcaaaatcg tttttatttg taatgatacg gctgtttttg
    33541 aaagaaagaa aatgtctgct atcttttgca tactgacata ccctttttga aacaataagt
    33601 acgtcacaat agttgaagct cggaaatcca tgctggaata gattttatgt ttcgtcacct
    33661 cttgtatgat ttgctgttta gtttcagatt caattgattc taaaaaataa cctgaacgtt
    33721 tatttgactg gataatgatt ttcttctcgt agatagcaag catatttttg atttcagttt
    33781 ggatcgtttt cttgttggta cctaaagctt cagctaattc ttcgcttgtt atccaatgat
    33841 tcttatttct taatactaca agaatttgga gttgtcgttt atttaagtcc atttgcttac
    33901 acctcctctt tgttataaac atacaggaaa aacctctcaa atgacaatag tcacttgcag
    33961 aggtttaaaa atcatttgaa gaaagatcta tattcaaggt ttttaccatt agactggata
    34021 atttgtttat aatattcaaa actatctttt ttcaatctcc taaagcttcc gctcccatca
    34081 ttttgtctat ccacataaat aaagccatat cgcttcctca tttcccctgt tcctgccgat
    34141 acgatatcga ttggtcccca ccataagtaa ccaatgattt ggcaaccatc ttttattgcc
    34201 tcagcaattt cctgaagatg tatttttaga tactcacaac gttttggatc gtggattcga
    34261 ttcaattcat ctggtgcttc atctagtcca atgccatttt caactatgaa taatggtaat
    34321 tcataacggt ctgtcaattc attcatgaca taacgaaacc ctagaggatc aattggccaa
    34381 ctccaaggtt cgggagattt ttccttcaaa aagggattat cgaaaccatt tgctccgcca
    34441 gtatctacgc gcatttctgc ttctttttta tagacagcgc tacgataata actaaatgca
    34501 atataatctg ccgtgttagc ttgcaagcac tgttcgtctc catctaacat tactggtgca
    34561 cagtcttctt ccttccatgt cctgtaaata taagctggat aatgccctcg gcacatgaca
    34621 tcggagaaaa accatgaatt gcgtttaaat tgcagtgaac caaatacatt ctctggatca
    34681 cagtcatatg cataagtcgc taatgcagaa aagctacaca taatacccat cttagcattt
    34741 ggatcgattt tctttaataa ttgagtagca cgggcgttcg ctagaaacat atgatgacag
    34801 gcttgataaa tcactttttt tgttagtgta gcaatgggtt caagtttatc tctagcaggg
    34861 aatgtaacca atccagcagc tgcataaggc atacgaaaag cattattgat ctcattaaag
    34921 gtcatccaat atttcacttt cccttggtag cgttcaaaga cggtctgcgc ataaaattca
    34981 aaaaagtcga ttaattttcg attttgccac cccccatact ctcctaccaa tgctaagggg
    35041 gtttcataat gactaagagt aattactggc tcgatatcat atcgatttaa tgtattgatt
    35101 agtctgtcat aaaatacgag accagcctca tttggcttct gttcgtctcc tcttggaaat
    35161 attcgtgccc atgagatgct tgttcgaaaa gcttttaaac ctaactcctt cagtaaacga
    35221 atatcctctt caaatcgatg ataaaaatca atcgcttcat gagaaagata atggagtgat
    35281 tcgtctaaag caatctccca aatctttttt tcttcattcc actgaatact aggatgctgg
    35341 ttcatgatcc cgataagcgt atctgtcaca ttaggtaatt tgccgtcttc caaccatgca
    35401 ccttcacatt gattagcagc tactgctccg ccccataaaa aattttctgg aaatatttcc
    35461 tgatccataa ctatctctcc tcgctatcaa caatttctta cttgaagtat agctcgattc
    35521 atcaaaacaa gatattctaa ctatttcggc agaagagaag aaaaaaattg aatgctactc
    35581 ttcattttta atatttatat ataacaaata cataagacga tccccgaaag aaaacactcg
    35641 ttaaccatct aagcaaacga tttattttac ttccaacaca aatgcttcat aaggtcgtaa
    35701 atcaactttg ccgtctggta actgtttcag aggataattt gacacaataa cccttaaagc
    35761 ttcacgtgga acagtgaacg tctgcttttc actggacata ttacaaataa cgagccattg
    35821 ctggtcttca tatatgcgag aataggcaaa gacatgctca ggcgtctttt ccactaattc
    35881 atagtctccc cagacgatga tgtcatgttt tctacgcaaa tcgatcaatt tcttgtacat
    35941 ataaaaaaca gaatcttgat ctgctaacgc ttcttctgca ttgatttctt tataatttgg
    36001 attgactcga agccatggaa ttccagtagt aaacccagca tgttcacggg cgttccattg
    36061 cataggcgtc ctagcattgt ctcttccttt cgcattgatt gattccaaga tttcctcttt
    36121 tgtaaatcca gaagaaatcc tttcgttata catattgatt gtctcaatat cttttgcttc
    36181 cgcgatatca tcgattgggc aattcgtcat gcctaactct tctccttgat agatataagg
    36241 ggtgcctttc atcatatgga gcaagatagc aaacatcttg gcactttctt ttcgatacac
    36301 atcgtcattt ccccaacgag aaacaattcg cggaagatcg tgattattcc aaaataaact
    36361 attccatcct tcatcaccta aagatgtttg ccatttggat aatacttttt tcagttcaga
    36421 gatactcaag ggtttcaaat cccatttgtc tttgccttct tgctgatcta atcctatgtg
    36481 ttcgaattgg aagaccatgg ataattcatt tcttttagga ttggaataaa gtttagcgat
    36541 ttcggtagtt gctccccatg tttcgccaac agtcatcaga tttttgtcac caaaagttgc
    36601 ttgattcatt tcctgaagat actcatgcag tttaggtcca ttacttgtga tttctttatc
    36661 cggttctttg ccaatcatat cgatcacgtc catacgaaag cccccaatac ctttatcgat
    36721 ccagaaattc atcatttgat aaatctcttg gcgaacctgt ttatttttcc aattcaaatc
    36781 aggttgacgc ttgctataca agtgcagaaa atattgcccc gttttttcat ccaactgcca
    36841 agcagaacca ctgaatattg agcgcaatct attaggctct tttccattga ccggatcacg
    36901 ccaaatataa tagtcccgat aaggattttc tttattttta cgggcctcaa tgaaccacgg
    36961 atgttcatca gaagtatgat tcaccaccag atccatgatg atacggatat ttcttttctt
    37021 tgcttcttca atcagttctt ccatttcttc catcgtaccg aattctgcca tgattgcttg
    37081 ataattactg atatcatacc cattgtcatc attcggagac tcgtatactg gagaaagcca
    37141 aattgcatca atacctaaat acgccagata atccaacctg gaaataatcc cccgcaaatc
    37201 tccaatacca tcttgatttg aatcttgaaa acttcttgga tagatttgat aaacaactga
    37261 atggtgccac catttttgtt ccatgatttt cctcctataa atggatgttt ctattttttg
    37321 cttaatcatt tattttagtc tctctcacta aaaattgtaa cgctttcaaa tagctattgc
    37381 accttgttat cggtaacaga ataaaacaaa aaaacagctg aggaaattcc ctcaactgtt
    37441 ttttgaaagg attgatttat gcagggaaga atgctgattt cacaacttct ttataaacgg
    37501 tgttcactca gctgtccctt ggtattagct caaaaaacgg caaaatatga ggaactattt
    37561 tttcgttttt ttctctaata ctcaggccag aacactgatt tcacaacctc tttataaacg
    37621 gtgttcactc agctgtccct cggtattagc tcaaaaaacg gcaaaatata agaaactatt
    37681 ttttcgtttt tttctctaat actcaggcca gaacgctgat ttcacaacct ctttatttca
    37741 cattcaatat ccacatgaat cccacaaata caaagaacag accatacatg attggagata
    37801 cttcttttcc tcttttagct gctaacatgg taattggata gaagataaaa cctaatgcaa
    37861 tcccatctga aatactataa gtcaaaggca tgccgatcaa gatcaagaat gaagggatcg
    37921 caatctctaa gtttccccat tcgatttgtc gcaaagattg tgccatcaat actcctacca
    37981 taatcaatgc tggtgcagtt acttgtgaag taacaactga tagtaatgga gaaaagaaca
    38041 agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat acctgaacgt cctccaacgg
    38101 cgataccagc tgatgattct acaaatgcac caacaggaga agtaccaaat aaggaacccg
    38161 caagcatagc tgttgaatca gaagctaaag ctttccctac acgaggcatc ttgttgtctt
    38221 tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc tgctgtatca aagaaagtca
    38281 caagcaagaa tgtcagcact acgacccaca tttgaagaga attgatgtcc cctacatgtt
    38341 ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc ggcaacaatt tcagaaggca
    38401 ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc tgctatcccg atgaagatgc
    38461 cacctggtac acgccgaaca agcaagatag cagttacgac taatccaaaa atcgtcaacc
    38521 aagtagaacc aacgttgagt gatcctaatc ctactaatgt agattcattc gaaacgatga
    38581 tcccaccttc gctcagacca aggaaagcga taaatagacc gataccgcca gaaatcgcat
    38641 atttcaaatc tgctgggatc gcatcaatga tcatttcccg cagcttgaag attgtgatca
    38701 aaataaaaat caaagaagca acgaatacgc cggctaatgc tgtttcccat ggaatcccca
    38761 ttcctaaaca aacagaataa gcaaaaaaag cattgatccc aagagcaggt gccgttccaa
    38821 ttggataacg ggctaatacc cccattaaaa cacagccgat cgcactggct aatgcagtcg
    38881 cagtaaacac tgctccttca tccattccag aagcacctaa tacagttgga tttacaaata
    38941 agatataagc cattgagata aatgttgtaa atcctgccag tacctcacgt tttaccgtgg
    39001 tgttcaattt ttctatttcg aagtaggaaa gtattttttc cttcatcttc catcccccta
    39061 aatccgaaca ttataaataa agtccgttaa aatttcgatt tattaataaa acaacgtgtt
    39121 ttatcatata ctaaaacaaa acataagtaa acaaaaaatt cggattttaa gcaaaatccg
    39181 aacgttccat ctgattaaat ccctttatct caaagttcat gaacattcac tttggattac
    39241 aagaatttct ttattgtgtt aataaagttt cttcattcta gtctcaaaaa tcaatagtgt
    39301 gatttgattt taccatttcg tttctgttct tctttgatga attcttttgt atcgacagga
    39361 ttcagattat ttagtccgcc aaaacctgta tcgaccattt ttccccagcc ttttgtcttc
    39421 ttttttcctg atttgagatt ctttgctcga agcttctcac tatgttttgc tcgccgatac
    39481 ttcaaataac cttctaatct tcccattaga tccactgttt ttcctccttt ttattcaatc
    39541 agacgactca gcttttccta taacaaaata attgccttca gggtcagaaa aattgaaagt
    39601 caagatgcca ttgttatctg tgatttctcc tggttcttcc atgttctcgt agatagtttc
    39661 aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct aatacctctg gtgaaaactt
    39721 ctgcacaaat acttttggga atacgcctaa ttctaattct tttgagatgc tgagtacaat
    39781 actcttaaat tcttctggca attcgattgt ttcagaaatt tctgcaccaa ttttttctac
    39841 aaagaatcgg caaatcatct ctacgtcatt gacataaagc atcacacgtg ttttttgtat
    39901 catcatttga cccttctttc tatttgattt tctttcctgt ctataaactg attccattat
    39961 atcacgaaag cgcaaacacc ttgtgtttga tactattcaa gaaaaataaa gcaatagttt
    40021 ctaggattgc tttcctccac aaactttttt tacaaaaaaa aaaaacggca atgaatgccg
    40081 ctcttcttat ttcgattctt ttataattct gcttctgatt catctttggt tagtgtgtgt
    40141 ctaacactag ttacgccaaa tgtttgatcg attttttcct tcaacgcttc gattgcctgt
    40201 gcgccacgtg ccgcttgttc agcattgatg gattcaatga ccagcaccgc tgcttgtgtt
    40261 tcttccgtca gcattgttct ttgggcttca cgccagttga gacagcgaca gatcgctcct
    40321 tgttcatcat agtagatgat ttcttccgga agtgccggag catcggaatc tgcacctaaa
    40381 ggcaggaatg attcgccacc ttgggctttt cctaaatgca gatttcctgc gatcttttcg
    40441 atatcttctc cgccgcaagg tacgccaaaa gacaaggaaa tactattata gatatctaca
    40501 agcggattga ttgggaaaaa ttcccgttct tgatttgcac gtttcagtaa agcttcaata
    40561 gaagaacgtg cacctttttt agtcttgaat gtggtaaatg cctgacgcca ctgagcaatg
    40621 acttcgtttt ggctgaatgt ttcttctgta aggtaatttc ttgcttcttt tgctgcctca
    40681 ttcaataatt gatgatacgt ttcttcactt tcttttgtta cgtggttatc gataccattt
    40741 acggataaga tttcgatggt tgcttcagga aatacttccc agaaattggt atccactatt
    40801 acttttttca tctgttcaca cctcttgatt agaataattg cttgatgaat agatagtctg
    40861 tttggggatc atctcccata taaaaaacat gttcgctgta tttgataaat ccttttttac
    40921 gataaaaagc gagcgcaaca tcgttgtgtt cccaaacacc gagccaaatg gctgtttttt
    40981 gcttttcttt tgctttttca atagctgtat ctagtaaaaa tgtaccgata ccttgatgtt
    41041 taaatttttt tcgtacatag attcgctcga tttctaaaaa atgatcagcc atttcctcag
    41101 attgactcgt tcctgtattt agtttcaggt aacccgcagg ctcttgatcg atactcacta
    41161 ggaaaaaatc ggtttcttta tcttttagct cttctgtaag tttttctagt gcataagctt
    41221 gtgtcaagta atcagctaaa tgctccggtg aattatcttt tccaaatgta tctgtaaatg
    41281 tttcgatact aatttgttgt agtaagtcta catctgaagg cgtcattttt cgaatttcgt
    41341 atttattcac ggcttcttcc tttcaataga tccttttatt gccttttttg acaaaattcc
    41401 aatcttcgct aacattcttg catactttat ttaaaagata ttccaattgt tttgcttctt
    41461 cgtcagaaag accatttaat gcgactgcat tggagtagtt gttttctcga atgatgaaag
    41521 gatagatttc tgctcctttt ttagtcggat agagtttctt gattttttta ttttctttat
    41581 cttccaaacg ttcgatcatc ccattggatt ctaatttctt gattgctcgg gcagtcgttg
    41641 tacgatctac tttgatcatt tctgctagtt tttcttggat gattccagga ttttcgcaaa
    41701 tacgtactaa ataaagatac tgtcctcttg ttaaatcgac ttctttgaat tcgatattgc
    41761 taatagaatc tagcgctcgg gcaatcattc cgatgtctcg aaggatttca gccatttcat
    41821 cccaccttta ttttgtttgt aagactagtt tacctctttt tgttgcaaat gcaactattt
    41881 tcaataaaaa aatcaagttc ttttttaata actagtaaag tatattcttt tcttctttta
    41941 cgaaaaagct caatcacaat agatctggtt tttgttttct aaaataagta actgtttctt
    42001 cgttgttcgc ttttctgata gctttggcaa ctcttctata ctaaaaaatc ccatgttgga
    42061 agtttcatta ttctcaataa acttggcctc ttcaccgtgg atcgcacaag caaaaacaag
    42121 tttataatac tgaaaaagct gtggaatatc tttttgttta ttcgtatcaa aaactgctcg
    42181 caactgagta gtttctactg ttaatcctgt ttcttcatag acttctttac gcacattttc
    42241 ttcaggagac aatcctattt cagcaaatcc accaggaagt gaccattctt tcgtccgttt
    42301 atcttcgatt agtaggacct tgccatcttt tttgatatag gcacgaacat ctacttttgg
    42361 tgttggatgt ccttctccaa tcgttgtgag cgcttttatt tcttcgaaag tttcttttcc
    42421 tatttcactg atcagttcca atgaaatcgt ccggatctcc tcaaaacgct ctttatcaaa
    42481 tacatctttt ccataatata gaccagcttg agaaagtgcg agtagtcttt tgtattgagc
    42541 caacttcttg ttcattctct tcccttcttt tttagcaaat gtatgactat gtaatcaagt
    42601 atagaagact ggtttaatta cggcaaataa atataatgaa atctgcaata aataaaagaa
    42661 tgttaaaaaa ctcgagaaca aaagacattc gacttctctg gggctgtgaa taaatggcgt
    42721 tcaaaagctt gcatctgcgg taataagccc caccaaacga aaaatatgac gagctattct
    42781 tgtctggctg ttcttattaa ttgaggcagg acagcttttt cacaacctcc ccactaaacg
    42841 gtgttctcct accaactcct cggtcacaag tcacaatatt catcaaacat gagaagctgt
    42901 ttttttgaaa ttgctccttg tgactcggag ctgaacgaca ggctcacgac ctctcggtaa
    42961 acagcgttca aaagccaatt tttcgatatt aagccatttc tttgttactt attgtcattg
    43021 atttcttttt ctaatttatc caatagtgga gtgtaatcca cttgagtata gtctgttaca
    43081 tcaaattcat atagtgttcc taactggaat ttgtcatatt ttcggtcggc gatgatcttt
    43141 ttgaatgctt ctttcgtaat atgattggtc cctttcgttc gatcttccaa tacgtcacca
    43201 tagtgataat gatccatgat atagctcaaa tgacgatctg attcacgatc acgtttgtac
    43261 cgacgttgac ataacacttc aaagtctgct gttaggcgaa tcgtgatcac ctgatatccg
    43321 tatctatctg ctaattcttg gagtcgtgct ttttgcttat cactaaaagg atactcggac
    43381 acaatcactt tctttcctgc ttccatatac aaatcaagtg ctttgtaata gaaaggataa
    43441 actttcgtcc tttcttgttc cgcccgttct tccaaattgt taaacccgat ggattctgca
    43501 tagtattcct tgatttcatc gagcgtgatg gcatacatat ctggaaatcg ttcccgtagt
    43561 ttattcataa gatatgtttt tcctgtacca ggactaccag ccagaagaat gaaatacttt
    43621 ttcatctcat ttatttccta agttaattgg actttccaag ttcataaaca gtccaatctt
    43681 atgcttgaca atttctttta ctgcttcatt tgctggaatc acattatggc gaagagactt
    43741 gttgatttct gtttcactaa aggtttcttt agctactttc gtccagttga ctaaaagctc
    43801 cgttccaaca ttgaatttgc ggatcccgtt attgatcaat tcaggataat cttcttcttt
    43861 cacacctgtt cctccatgga taactagggg gacatcaact actgcatcga tttctttcag
    43921 caagggaatg tttacttctg tttttgattt gaattgtcca tgattcgtac cgatagcaat
    43981 agctaatgca tccacgcctg tagcttctac aaatttcact gcgtcttccg gtcttgtata
    44041 taccttgtca ttttcagcga catggattcc ttcttctgta ccgccgattg ttccaagttc
    44101 tccttctacg gaaacaccgt gcgcatgggc atattctact actgcttttg tcttcaggat
    44161 attttctttg aaaggcagat gggacccatc atacatgaca gaagtatatc cgctgtcgat
    44221 tgcttctttg atatcatcaa agtctcgggc gtggtctaag tgcaagactg catcaaccat
    44281 ttcttcttct gcaatagctg tcattacatt gactaacaca tgcatgccga tatatttagc
    44341 ggtatcgaca cttgtttgaa taatgatcgg cgaacccatt tcttttgctg cgcctagcat
    44401 agcaggaagc atttctaggt tatgcgtatt aaaagctcca actgtaaaat tcaactcttc
    44461 tgctgttttt gttacttctt tcaatgttgt atacatcttt tattgccacc ttatcttttt
    44521 atttgtttcc tttgatcgtt acttgttttg caatttgtcg catctcatcc attttgccca
    44581 tgtagtaatc gatcccttga tcatcaccag cacgagcagc atctgcacgc ttctggttat
    44641 agaggctcat cagtttttta tacccatctc cgatcgtctg tttattttcc aaactctttt
    44701 ctaaatattg gatggcttga tcgttcacat acaattcaga aaatttaagt ccaacttgtt
    44761 cataaagttt cgctaattta tgcttatctt gggtagtgga aatctcttgt ttcaattgtg
    44821 taatctctga ttgaatcttt tcggtcgctt ctggcgatag ggctttttct tcttttgcca
    44881 gaactttttt cggttctttt tcttttttct taaaaaatcc aaacatcatt ttccctactt
    44941 tcaattaaaa tgctttcaaa actgggctta agatccaggc atataagagt gctgcggcta
    45001 ctgtatctac atcggttgct gtagcattga tgaatcccat cgaattaaag atcgttacta
    45061 gcaaagcggg caataatgta ataaagaaac catgagcaat accgccaatg atcgctcctc
    45121 ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc tccggcaaag aaattggtta
    45181 gcataccagg taaaatcatc gctaagccga acatgggcaa agtaaacatg gcaataatcg
    45241 taccaatcgt tgtcgtaatg aaacctaaaa tgacagcatt tgggctataa ggaaagaaaa
    45301 ctggacaatc taaggcaggg atcgcatcag gaactaattt catagctatc cctcgaaatg
    45361 ccggcacgat ttcacctaac agtagacgta caccagctag caatacatac acacccacta
    45421 cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt ttgatctcct gataattcag
    45481 aagcgaatgg ttcgcctgca aataaaacag tgatgatata tagcgggacc atcacgacca
    45541 tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt cggcaacttg atatcttcga
    45601 tcgacttttt attttcgcct ttctcaccaa aaagcttagc aacaccggct tcaaataagt
    45661 acccgatcgt acaaaaatga cctaaagcaa tatcatttga tccagtgatt ttacgaatga
    45721 tcggttgcgc tactgccggc atagcaatgg cgaaacaagc tccgataaaa ccaccgacca
    45781 agatcaatac gatccctcgc agaccggcaa aatagccaaa gactgtcgtc atcgttgcca
    45841 tccataaaat tgcttgacca gttaaaaaaa tgtacttcca ttttgtgaaa cgagccaaaa
    45901 taatattgaa tacaaaaatg gctaagaacg tcagtgcaat atctctacct aaacccaaat
    45961 cattcattgc ttgaccattg atagattcaa tagaagggat gatcccttgc atatgaaatc
    46021 cttcagtaaa gattttcccg aaataagtta aacttccaac gatgatgctc gaaccggcac
    46081 tcaacacttg aaatcctagg agtgtcttta acgtgccaga gatgacttgt cctgctgatt
    46141 tcttctgcag tagtagaccc aacatagcaa tcaatgcaat ggtgatcgat gcctgagtca
    46201 aaatattttc gatgataaaa ttgataacgc ccatgttttt accctctttt ctttttgtat
    46261 ttagataact cccttatctt gcagaactgg ggtgattttt tcttcaattt cattttttga
    46321 aacaatattt ttcaaataga tcatcgccgt tttaggatca actgcgaact tttcgaactg
    46381 cgaacgaaaa ttttctgctg tgatgatcac atctggtttc atggaaactg cggaagaaat
    46441 atcacaatgg tccagtttgg cttctactcc taatttgttc aaaacatcct cagcagacat
    46501 ctgtgctgca aaactacttc ctaaacctgc accgcatacg aataaaatgt tcaatttgtt
    46561 catctttgtt ctcccccgta tcatttatta tttctcaact actgaaaaaa gcagttgttt
    46621 gaaatcttcg atatttgtgc attcaatcaa tcgatccaac ttctcttcgt tgttgatcaa
    46681 ttcaattagt tctttcatga tgttcagatg cgagtaggag tctactgctg ccaaacaaaa
    46741 aataatcttt actggatcca tttcaggatt accgaaatcg attggttgct caatcgtagc
    46801 tacgctgact cctaatttat tgaccccatc ttctggtcgg gcatgggcta gtgccatgtg
    46861 tttgccaatc acgatatacg gtccgtaatc attcactgca cgaatcatcg cttcgacata
    46921 gcgctcttcg acaatgtttt ctttaaccaa tggtctagca gctagttcaa tcgattcctc
    46981 ccaagtagtg gctttttctt tgatcatgat attgctgtcc gtcaaaatat cttttaacat
    47041 aggctggatc tcccttttgt tgatttctaa attgtttgtt tcgaataatt tttctaactt
    47101 gccatagatg attcgatcta ctcggccacc actttctttc atgacatcta ttatttgagt
    47161 gaataactcc gtcgattcat gactgttagg ctctaaccgc tgtagttctt catttctttt
    47221 caaaaagtct tctataacca agatattcgt ctcagttaaa atcggatcga caactaaaag
    47281 cggcttattt cgataattca attgaaaggt gctgaaaacc agattgacat ccaatttatc
    47341 tactaattcg atttcttttg aacttaaaac tgcagtaatc tcgatctgag gaaacttttc
    47401 tttcagattc tctgctaaca gatttccagt agccatccca tggttacaga caacgactga
    47461 tttataaaca taattcaagt ctcgcttgat tacactggcg atcgtcgaaa agtggatgac
    47521 aagaaaagct atctcgtcct ccagtatctt gccaccaatt acttcttcga tcgtaggaac
    47581 aaaatgagtg actgcgcggt agattgggaa ataatttgtt cggatgtttt ctttcaatgg
    47641 attcatgact tggatatgat tcttgattct tacaatcagt gcagccatgt gcttatagag
    47701 attttcgaat aaagcttctt cctttagatg gaaaggtacg tgtgtctcgt tctctacaaa
    47761 ttggatcaat tggatcgtta gtaattgagc ttgtacccac tcgattgaat tggagatatc
    47821 tctagcattg aacgtctcaa tagtgaaacg aagataattt ctttctactt gcgggacatt
    47881 gtccaattga aattcctcga tcatgtggtc aataaatgca gaaaagctac tgtcttttat
    47941 ttctgtattt ttccaattaa tggcagagat aaattcatgt ttcctgattc gctggatcca
    48001 gatcaatgta aaaagcagga tttgtttttt ataaatatca tcttcttgtt tcaacaactt
    48061 attggaatag atctcctgta tttttttgat ttccaccaaa ggaaaataat ggagaaagat
    48121 tttttgagcc ggtgttaatt gtgcatcttg attcataaag aagtcggtca ttcccagatt
    48181 ctgattgata aaatcataga tcatcgtacg gatagatcgt tctgaacctt tataaaccag
    48241 tccttgcttt ccataactga cgatctctat cccataactg accagtgttg ctcgtaaccg
    48301 tctcatatct tcatctaatg tacttttaga gatttgaaat tcttcttctt ttttgtttag
    48361 atggatcggg attcggctgt aagcaacggc taaaactaga tcaaaaatac gttcgcgccg
    48421 attcaaaaag ccgtctttca catcttttat ttcagtttct aatcgagctt gcttttcctt
    48481 ttccagcttc aacaaaaaaa ctttgttgcg aattgtacgg atctccggaa gctttttact
    48541 gctcaaccag atattcgctt caagaacttc ctgacggatc gtccgctgac tgacattgaa
    48601 ctcacttgct agatgttttg cttttactgg atattgggct gtcatgattc tgtgaagtat
    48661 ttcaattgtt cttgttttca tagaaatgat ctccttctga tttcaagtat atggttccgc
    48721 ttacataata aaaagtagat atttctgcca cttgaagtgg aaagaaagta agcaaagcaa
    48781 acaaaaaata agccatatgc tttttcatct cctcaacgaa aaagacatac gcctattatt
    48841 acaaaaattt tgcccatcta tacaaaaaag aggttgtgaa aaagctgtcc tgcatcaagt
    48901 aataagaaca actaaactaa aatagtgctt cctatttttc gtttggttgt tcttattatc
    48961 gcagatgcaa gcttttaaac accatttagt ggagaggttg tgacagagat gttcagcctt
    49021 gagcgataag aagcgatttt gaaaaatcgc ttcccatatt ttacaaaatc gtgccttatt
    49081 gccgataggc tatctctggc acactgttta ttcagaaaaa ggagctgcga caaggctttt
    49141 gtcacaactc cgttcataaa tatttcctgt caatccagca taatcggctg aataaaatcg
    49201 gcttgccatc ttttttcttt ctcagaaaaa tcaaaatacc atatctgaga aaaatactgt
    49261 cgttgtgtct tacttcctga gatcaatcgc ccatcttttc gatcgactga ataatcacag
    49321 catctgaaat caattcggat ggcaaaacta ttttcgtgga tctgtcgata atttcctact
    49381 tcctgaatag ttattttttt agtatgattt cttacaccgt ctgctttgtt tttttgcaga
    49441 actttctgat gttcggcaaa cagcttgtct gtataacagc ttctggcttt ttcaggagtt
    49501 tcttcttccc aagcggattg gatcttcatg aatatctttt ggatttccag aaactgtgct
    49561 ttcttttctt tagccgttcc tgacatgcgg gacaatatct ccgctttcag ccttttcttt
    49621 tgtcttttat tcttgacgat agaaaaagca gaaacccctc ctactgacaa aacagctaat
    49681 gcaagaaatc ccgtttgctc ataactagaa ggacctgtac ctggaccata atacccataa
    49741 gaatctctgc cgtaagaacg actgccaaaa taactgcccc catatgaacg accaccaaat
    49801 gaacctctgc ccgaacttcg accgctagat gtacctccac catgaccacc cgcgacagcg
    49861 aatccttggg tcggcagagc aaacaaacat aggcccacta acaaaatgat tgctaaataa
    49921 ttgatttttt tcattgtctt cctcgttatc ttttattaca aaaaactatt tttatgatag
    49981 ccttgctaaa aggaagagtc aataaccaga tgcataaact gtttttttag caattttgtc
    50041 ttatttagtc attgagtaac atcggacaaa tgtatgtgtc ggcagtccat aattcctcta
    50101 tcttcagcat gaacaagctt gcagacaaga gatctgcgct tcctccagga gagatttggt
    50161 tttcctgaca aaagtgcaac agtgctgtaa atggacgatt tggtaaagca ctttgactag
    50221 cagcatgagc aagtttttgc atttccacta acgttttctg atcactgcga tgcaatacgc
    50281 atgtgtcatc tagcgaactc atcaaaacta acaacatatg catttgtttc tgtacatatg
    50341 aatcattatc tagctcctta gctgcttgga ttgcaagcct gatatgcgga taccctaata
    50401 tcgcttcttc atatgcacca tttacgccat atttttgctt caccctatga ccatgagtgg
    50461 tttttttatg acgatatttt gtatcaggaa aagcagcaag cttgctgaca tcatcaagta
    50521 ttttcgataa agaaaaaggc tgttccatac tgattttttg tgcaatgaca ctgatcaata
    50581 gccccattgc ccaaatcgct cctttatggg tattgatgcc atttgtagca atcagcattg
    50641 ccttttccgc ttttcgccca atctcagcaa ttctttctct taactcctga tcaatagcat
    50701 gatgccatgc tatttgagcc atttcttcaa aaaaaggagt tagcgtctta gcagacttaa
    50761 gaaataaagc atagtccata tcggaatgtg cacccgcatc cagtgcatcc actaatccag
    50821 gttttggact taatgttact tctgcgatca atgcttcctc taccaacgca gcgagttgct
    50881 gagtcactgc tttttttcgt tcgtcattta tcatgacact cactccttct tcctactatt
    50941 acttgctgaa accttttttg atagctaccc aaatgatatg aagtaaccag cttaaagcaa
    51001 atgcacaact tgccgtcatt agataagcta caagaatcat gactcctgaa ggtaactgca
    51061 gccaactatc tttaaacaat aataacagta cttggaaaac aaatacattg gctaaatacg
    51121 tacgataagc ataaacagac aaccatttga acaatggcaa ttttggcgaa tcgtttacaa
    51181 tcattttgga agcgcctatg aagacagcaa agatgatgac taaactgtat aaactttgga
    51241 tcgtattcaa gtaaggtgca ttcgcaaagc tcaagtctcc tggatgtgcg agcaatgaac
    51301 ggacagaaaa gaacgcgata actaaggcaa caggtaagaa aagataacgt actctgtcca
    51361 aatattgata gactttttcg tgataaacaa atgcggctac cccataaatc atgaaactag
    51421 caacaaaccg atctaataag taccagctgt catgataagg cccagtcaat acttgtgttt
    51481 gatagaacag ataccatgcg acatataaaa tcgttgaacc aatcaatact ggccatacat
    51541 attttttatt agcaaatact ttgtaaccga gccaaacaaa aaacggcatg aacaattgga
    51601 tctgcagcat cataacggta taccataaat gaggcgctcc atcacctgtg acatatttca
    51661 gtaaaaagct tgcagtatcc gtatacggag tttgttgttg gactgctgga aatagccaaa
    51721 gataagccgt cgtccataga agatacggaa cgactagttc aaaaaacttt tctttcaaaa
    51781 attcccaata agacaaatgc tgacttgttt ttaccatatt ataaacaatt gcaaaaataa
    51841 acattggtgc tgtgtattta acgatgacgt aaaaggtacc aagaaaagct gtatttgttg
    51901 cgctgtggct gtttgccaaa gcaaagctca atatcgtttg catcataaca gccgttacag
    51961 ctgatacttt ctggaagtca cttacgttga ttttgatttt tttgttttca aacatctctt
    52021 tctctctctt tttctttatt accaaagttc tttggttaat ttaggtcctt cgactgtttt
    52081 caatagaatt tttttctctg gctctctcaa atactctttt agtgaaaaac cgccttgccg
    52141 agtcactact tgggtatcga attgctcaaa aaaagcagga tgcgattgaa ttacctgaat
    52201 tggcaattca atcggtgaat ttgcgtacag cagcaaatca aaatcactgg tttcttttac
    52261 tgccggaatc ccagtcgata attcaaatcc taagctgccg ccgattcccc atttacatcc
    52321 tgataatatg tttctagcag catgaaattg ctgataaaca ggataatcca tgattttctc
    52381 ttgtctatac gattcttgtt cgatgatgtc ccacggatga atgacttgtt tgacggcatg
    52441 ttttgagatt tctgttgacc atctctgatt gcgggcaggt ccacgtaaac caatcggtag
    52501 tatttctgcg tgatccgtcg acgcacgacg aactgttccg aacatctttt cagttagaac
    52561 gacccattca ggcaaggtga acccttctat ttcatgttta tcaaaaagga caatatcatg
    52621 tgcttgaatc attttttatg catcccattc ttcgtccatt ttggcacgga ctttatttgt
    52681 ttcgacacgt ccgaatgttt tggctgtttc gtttgtgtat cgatttgtca gcatcgtgtc
    52741 atttgtattg cggacatctt gaatggcttc attgattgct tcgatgactt tgctgacatt
    52801 ttgttctgaa gcagcttggt cggtgatatc ttctaggaaa tgatagatcg ctcctagttt
    52861 tgtataatta ttgatatcgt aagcgatagc tggaactttt tcagcagctg cttcgatttc
    52921 agcaaccgaa cgttttgtca cacgtgcagc actttcttta gacatcgctt ggacggtaat
    52981 ttctttattg tttaaagcaa tcaagcggct tgattgtaat ccatgtgcta aaaatgcacc
    53041 tgaaatagca tttcctgcaa tcaatccaat aactggatgt ccagcttgac ggagttttgc
    53101 ataagcagca gcactattag ccaaagccac atggataccg atcaattctt cgttatagcc
    53161 gtatgcttgg cttggtacat cgatcacaac gatgattggc cgtttcactt ttttgtcttt
    53221 atcttcttcg taaacgtgat ccaaaatttg tcccatgcgg aaaccttcaa ctaaaccaac
    53281 ttcaccgttt cgtacacggt acatcgggtt tttaggatca ggtacgattg atgtcacgat
    53341 ggcttcttct atgaatttac ttgatttacc ataatacaca gtactaatag aagaagttgg
    53401 atttttcatg ccagttagtg ccgcaaacca atcgtaacca acacttgctg cagctggttc
    53461 atttccttct gctacttctg gtaagtcaat tgattttgct tgatggttag caattgcttc
    53521 attgtaatcg tcaatggcca ttggttttga aagatctaaa gaatcaagta atgataaata
    53581 aaatgctgag ctttcactgc gatgcgtgtc ttttcgttcg tccattgctg cgatgacagc
    53641 agctttgatt gcttctacac tatcagtcac taattcatca atgatttttg attctacccg
    53701 ttgacgagat cctaatgtat tccaaatcaa gtctttgtca ctagaatcaa attctcgaac
    53761 gcctgcttct tgttcaataa cttctggtcc attcaaaccg actctcgctt tttttgttgc
    53821 gattaaataa ctcattaatg ctgaagtaat cgacataccg ccaaatgatc ctacacgtcc
    53881 tggaatcaaa ccgatgactg gtacatattg ttttagagca atgattacat ttccgatttc
    53941 tgaaatagat aacagtccgt agtttgcttc ttgcaaacga acaccacctg tatctagtac
    54001 aatgactgga aagatctcat ttcctttttt attttcttct aacacatgtt caagtgcagc
    54061 aacgattttt gcaccggaaa cttcaccgat accgccacct tggaattttc cttccatagc
    54121 gattactact gcttgtttgc cattgatcgt gccacggctg acaacgatcc cgtcatcact
    54181 ttcagggacg attccttgtg ctggtaaact tggcgcgatg atttgatcga atggatcaat
    54241 caattcacgt ccattgtcaa gtagagcgtt cactcgttct ctggcgttta attctacaaa
    54301 actatttttc atctgaaagt tcctccattg cttgcgtcaa tcgtaggtac acaactcctg
    54361 gtgtagcacc gaagtcattg atcacgatat ctgctgaaat tggataacga tcaaaaaatc
    54421 tcgttaatac attgttccat acttcgccga agccatcgct tcctgtacaa atttttacag
    54481 tagattctgt tttttctgtt ggaaacatca agatttccaa atctcctgaa ccgaccactc
    54541 ctacatggat tttcttattg atcggctgtt tcgttggata tttataagct aattgttcca
    54601 tggatctctc ccctttttgt aaaacagagc atacacgttc tgcttattcg tctttcttct
    54661 tccatctggg taaatggcga caaaaggttt ttctcgccaa ttttatgacc aagttctgaa
    54721 tttcattggt ggttcataca ggccatctga ccattcgatc aaatcatcga tggtttgtgc
    54781 agctaataat gaacgtttcg cttcactacg tttgatgcct agatcttctg gtaatttcac
    54841 gatgccggct gcacgcaagc tatctagtga ttctttcgtg ctagtcattc caagaggtgt
    54901 aacaccgcca attgctgcaa ttgctgcttg tcgttcttct tttgtttttg ctttgtacaa
    54961 ataagcaatt ccttcttctg tcacgatatg tgtagtatct tcgctataaa tcatcactgg
    55021 tgcattggct aagcctgctt gtttttgaac agctagtgca tctaattctt cgacaaagac
    55081 tggtttttta tttgaaccat atgtttcaac catttgaaca actaattttt ggcctttccc
    55141 taatggatca tcattgtcgc gaagtgattg ccatgctggt gtagaatgac gacgaccgcc
    55201 aggattatgt cccatgttag gcgcaccgcc aaaaccagat aaacgtcctt ttgttacggt
    55261 agaagagttt ccttcgtaat ctaattgcaa ggttgaaccg ataaacatat cgatggcata
    55321 ttgtcctgca gcttgtgata atgttcggtt actgcgcatc gtgccgtctt taccgacaaa
    55381 aaagacatct gggcgagcag caatatattt ttccatacca acttcgccac caaaactatg
    55441 gatgctttca acccaacctg attcgatggc tggaatcatc gttggatgtg ggttcaatac
    55501 ccagttcttc gcgattttcc cttttaaccc tagtgattca ccataagttg gcagtaacaa
    55561 ttcgatagct gctgtattga atccaatacc gtgattcaaa gattgtacgc cgtgttctgc
    55621 gtagattcct ttgattgcca tcattgccat tagaatttgc aattctgtga tgttttgagg
    55681 atctcgtgtg aataaagctt ctaattgata aggtttatct gctttcacga caacgtctac
    55741 ccagtcacca gggatatcta cacgtggtaa agaatcgaca acttcgttga cttgaacgat
    55801 cacgatacca tctttgaaag ctgcagcttc gatgattgtt ggtgtttctt ccgtattgaa
    55861 acctgtgtac aagttgccgt ctttatctgc tttatctgca gctactaaaa caacatttgg
    55921 aatcaaatcg atgaataagc gtccataaag ttccaaataa gtatggatat cacctaattt
    55981 cattgtgccg tcttcaatca tttgggcgac acgcaagctt tgtgcaccag cataagagaa
    56041 atcgatcttg cttgcgattc ctttttcaaa aatattcaaa tgttctggtc gtgagatact
    56101 agacatgatc aaatgcagat cattcacttt ttctgcatct actttttcaa gactttctga
    56161 aagaaaactt gcttgttttt gattgtcgcc ttctaataca actttgtcgc cgggagcaat
    56221 cagtttttcc attacttcta cgattttttc agtttctaca tacttgccat tgagcaagtt
    56281 gctgattttt tccaatcgct gcgctttttt ttcgcgttta gaagcccagt tgcggtttgg
    56341 tttttccata attagtcatc tccatttttg atatagaaac gtggcgtcat caataccatg
    56401 ttggtctaga ttgatcgtac ggatcccttt atctacacca aattttgtat gaatcagctt
    56461 tgtcaaagta ttgccggggg gaaattcaat cgtcagatcc attcctaatt cttttgccaa
    56521 gtccatcatt tggttccact gcacagggaa agctaggttt tccaacaaat ctttacgaat
    56581 ctcttctgcg tcctgagtgg ctcttccatc cgtatttttt atatacaaac atctagcatc
    56641 agatagatgg acagactgca tatacggctt gaatttcttg accgtatcat tcatcaagag
    56701 acagtgagat ggcacaggga ctttcagcag cttcgctgtt tgcgcttggt gccgtttggc
    56761 ttctgccaac gtaacggata aaccttcgat actcccagat aacgtatgtt gcatcggaca
    56821 attttcattt gacagataaa ctggatggtc aggatcaaat gtttttgcta caatttcctc
    56881 agcttcgctt ctcgtcacac ctactactac gcccattgca taaccgcttg gataagcttc
    56941 tttcattaag actgcgcggt gatagactag ttccgctgca ttctcaaatc tcaaagatcg
    57001 acaagcaaca gctgcacaaa aagcaccaat cgaatgtcct gctgcaatat caggctgaat
    57061 accgtttttt tccatttcat ctaaataaaa tactgctttt gtcagtaatg ctagctggat
    57121 aaaaaccgta tcttgatagt tttcttcttt atcaactaac cgataccccg tcacattttc
    57181 taaacgttcc atgtacattt cagggatatc cgctaacatc cctaacttct gactgccttg
    57241 tccaggaaat aagaaacctg tactcatgag cctcgcctcc tcagcactta ctttttccac
    57301 tgctatctta gtcaatttct gctataataa aaaatgccaa aaaaagaaaa aaccataaca
    57361 aaaaggtatg gatgggatgg ataattatga aattgcttca tctgcagtat tttaaaaaag
    57421 tattcgaaac aaaaaatgtt acccaagcag ccaaagaatt atttatttct caacctgcct
    57481 tgagccgtgc gatcaaacat ttggaaaacg aattaggtgt ccctttgttt taccatagcg
    57541 gcagaaacat tgagacgacc gtttatgcag aagaatttta tccatatgca gtcaagacat
    57601 tagacacatt agaagaagga atcaaattat tgtcttcggt caatgagaag atcgttactt
    57661 ccgtcatatt gtatttagag gtagcctctg tttctattcc gaacttggtc cgaattttta
    57721 tggaaaagca tcctgatatc caattaacga tcatgcaaca tcatttacca gatgatatta
    57781 ccgatcctgt actgtacatc acttcggaga tgaagcctgg catgaccaat attcctatca
    57841 tgaaagaacc tgtttatgtc gccattccta agaaacatcc tcttgctaaa aaagaacatc
    57901 tgcaattaga agatatccaa cgcaccccca tcttgatgct gtcaaagagc aacgccttcc
    57961 gtcatacact ggatgcagca cttgaagcga aaagtatcga attgacgatc agttcgacga
    58021 cagatgatcc tgcgacatta cgttctattg tcaagcaagg attagctgtt agctttttcc
    58081 caaaagtttc ttggtcttac gataaaaatg atcctttcgt cttgcgggaa atcaaagatt
    58141 tgcccttgac acgaacaatt tatttatctt ctagcttcac agaagatcat cctttgatca
    58201 aaatgattgc aaaaacgatg cgggaatttt atttaggaaa ataaaaaaaa aagagctggg
    58261 acaaaattct tgtcgcagct cttttttcga acaaacggtg ttccagaagt aatttcttag
    58321 aaaataagcc gaaatttccc aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    58381 cttgaagcta aacatttctg tcacaacctc ttctctattg tcctagacat tcattgcgtt
    58441 gatttaaaac ttccatcaat caaatctgct agttcatcta gcttttctat tttacgtaga
    58501 tcaatagaga tccaatgttc tttattcatg tgataggctg gaaaataccc ctcttttcct
    58561 cgcaaaattc cgccaagttc aggatcgacc ttcaaatcaa gaatattgat gatcctatgg
    58621 tcacttgcat catcatagat tttttcttct ggaacagaca tcaggacccc aaaccatttt
    58681 ttgctttttt gatgtctgaa tacgcaataa tcaggaaatt ttttaaaaat atattctgga
    58741 gacaaattgt atgtttcatt gatgtgatcg attagttctt ttttcgttag catctgcttc
    58801 catctctttc ttattgattc gctttattca aagataccac gtaagaatag aaaaatgaat
    58861 acaagtagtt gctaatcaaa tacttcgata tttttctttt ttatcaactc ttaaatgttc
    58921 aatccgcctg tgttggtctt gctaccatct actattaaaa tgtttattat tgtttgccca
    58981 cctatttttt gattttagtg gtatcatttt tcaagttcat gatgaaattc ttatcatcag
    59041 taaatgctcc agaaaaataa acaatgattg aaaaatcagt tgaaacttga ccaccgttta
    59101 ttttctccgt attcttagaa tagaagggag tctgattgat gtattcacta atgaaaaaaa
    59161 tcattaccga aaaagatatt cgacgtcatg tttctttagt agaacaattg ctgaatcata
    59221 caaagataac cgtaaatgaa ttagctgaaa tcatagggac aaccgaacga acgatttttt
    59281 ctgaccttca atctattcgc tctcagctgc ccgaaggctg ggacatcatc agcgatcaag
    59341 caggaatcag tttgcaaaac cagcaaaatt tattaacaaa tgacctttgg gagatttttt
    59401 tcaaacaatc agtcagtgtc gaactgctga aaaatctgct ttttacaaaa aaagttgctg
    59461 tccctgattt tctagccgac catgggcttt cttatgggac attgaaaaga catgtgacta
    59521 aaatcaatca gcgtttagca agttacgact tgcagatcga tttgacgaaa tatacagctt
    59581 gtattcttgg aaaagagcgt gcgattcgta ctttttatca tcgattgctg attcctttca
    59641 ctcataacaa ctattttttc gaggattatt cgatccatga atcccattat tttcaatttt
    59701 taagaaacct cagtcaaact gaactagcgg ttgaaacaga agagattttc ggaacatgtt
    59761 ggttctttat caatacgatc cgtataaaag cgaattgccg attagattct tctatccata
    59821 ccaacagcac actctcctcc ctgtatgact ctgcattgaa aaaattgtat ttaacggaag
    59881 gtatttatct taaagacact gagctttctt ttgcttcttt ctgctttctg gaaagttgga
    59941 attacaacaa taactatgga caagaaaccg ctcgctgcct gcatcattct ccatttttag
    60001 aggtcctcga aacgtttgta gaggaactag cttcagagct gtctcttgat caactaaaaa
    60061 aaacatctct gactgataac ttggctctcc tcatattaaa atatcatgaa tcgccgatat
    60121 tgattgaagc attggaccgt caataccatc gttttttaga aacatatgat cagcaatatg
    60181 cacacttgta tcaacagaaa gagaatttac tgaacgatct aaaaaaagaa atagaaatca
    60241 atgaaccaaa ttactttctc caactcttgt ctctactgat ccaacaaacg attttttcta
    60301 tcaaaccgaa tttgttcaat gtctatttta ttttccaagg agaaccttca tggaaggcgt
    60361 tcttgcagca ggaattaaaa gattacttag gaaaacgtgt ctgcttctta cctatcgaac
    60421 taaccgatct ttccactatt tcttttgaaa aaacagatat cttgatttcc aatttccctc
    60481 tagattctct agatatccct attgtataca tttcttctat tccaacgaaa aatgagctca
    60541 atcgtttgac tgaactcact ttcaaaactt ttttatagta acggctagcg actgtattct
    60601 ctagctgttt tttctttttt gcgaaacagt ttgttcatga tccctttttc tatttctaca
    60661 actaagaata gaagaatcac atttgctcca atcaataact gttgtgtcat cgttaaactt
    60721 actgtaccga tcagctgctg gccgactggt agaaaaataa ccatcccttg caataaagca
    60781 aggataccta atgaaagaaa aagcgctttg ttttgcaaaa gaccttggtt caatgacggc
    60841 ttggacaact ctctgcaatt gatcatgtaa acagcttgag ctaagacgat attttgcaga
    60901 agaatggttt gctgtaagct ttgcccttca aactgcatag ccaataaata ggcaggaacc
    60961 atgatcagta gggagacata gatgatccga aacaaatgat aaccagatag gattccttgc
    61021 tttggatctc ttggtgggcg cttcatgatt tctggatctg gcgactcgaa ccctagtgca
    61081 taagataaag tgattgtcgt taccatattg acccataaaa tctgtactgg ggacaaaggt
    61141 agcgggcgat tcagcataag tgcccaaatc aagatcaatc cttgagctaa tgcagttggc
    61201 aagaagaaat tgatggtttt ttgcaaatta tccaaaatcc gccggccctc ttttacggct
    61261 tttgcaatcg tatggaaatt atcatctcct aaaaccatat cagctgcctg tttgcttact
    61321 tcactacctt tgattcccat agcaattcct acatcggatc ttttcaaagc aggtgcatca
    61381 tttacgccgt ctccggtcat tccgacgatt tctccatttt tctgcaaagc tttaacaatt
    61441 cgcagtttat gttctggtgt cgttcgagca aagacatcga ctattggcac ttgctgaatc
    61501 aactcttcat cagacataag atccaaatcg atcccttcta aaactttggc agtatgtttt
    61561 aagccaattt gttccgcaat cgcttttgct gtgtctttgt gatcccctgt aatcattttc
    61621 actgagatcc ctgcttgttg gcattccttt acagctttga ctgcactttc ttttggcgga
    61681 tcgatcaatc cggcgatccc tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc
    61741 tgtttcgaat ctactttttt ataagcaaat cctaaaacac gctgaccttt tctcgcaaat
    61801 tctgcagctg tctgactcca tgcttcttgt tctgcagaag aaagagttga tttagcaaat
    61861 aatacttctg gtgcaccttt gacgtaaatc accgctcctt tttcctccgc atgcatcgtt
    61921 gccatatatt tgtattcaga actgaacgga atctttgctt ctattttttt caaaggtaga
    61981 ttggctgttt ctgtgtgtcg aatcagcgca acttctgtcg gattgccact taaattttct
    62041 gttttctgtt cttcttttgt ttttaattcc tgacagtttt tcataatgga caacacgcag
    62101 gcttcttctt ttgcagctac atccacaact gtcatttcat tttttgtcaa tgttcctgtc
    62161 ttatctgaac agatcactgt catagaacct aatgtttcta ccgaaggaag tcctttgatg
    62221 atcgcctttt cttttgccat ttcatgtact ccaacggata gaatcatggt caaaacagct
    62281 ggcaatcctt cgggtaccat tgacaccacc aaagcaatcg tagccgaaag aaggatatgc
    62341 caatccatac catatcgaaa tgtcgtaaaa ataactaaga ataggctcaa acaaagaatg
    62401 ccttgaaaaa tttgtttgtt taatcggttg atttttttaa tcagaggcgt cgtttgttgt
    62461 tcgaccgatt gcaatgcttg attgatttta ccgatttctg tcgtgtttcc tgtttctaca
    62521 acgacaccga ctgcagaacc agattgtaca agcgttcctg aaaaagccat atttttctgg
    62581 tcgccgatcg attcttcttc tgtcaaaacg gcatgacttt tttcagatgc ttctgattct
    62641 cccgtcaaaa tcgattcttc cactaataaa tcatgtgctt cgattaatcg gatatctgct
    62701 ggaacgacat ctccggcacg taagacaatg acatctcctt ttaccagttc tttcgcaggg
    62761 attttttctt gcatcccgtt tgatagtact atcgcttctt gtcccatcag ctgcttcaaa
    62821 ccattcaatg attcttccgc ttttctttct tgccagtaac taacgagtcc attgacgatc
    62881 actacgaata aaataatact tccttcaata tattcactag aagcaaattt caaaaaagag
    62941 gcaatgatca acacgatcat cagcagatca gtaaaatgct ttgctatttt tttccatcgt
    63001 tttgtctgct gccttgcttt tatttcatta tatccttgat cagtcaggcg tttctgtcgt
    63061 tcttcttgcg acaagccttc tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc
    63121 aatctgtacc agttcatcct atcccctcct gtttaattga tactgttatt atacgcaggg
    63181 aagttttttc aggcgtatcg attatttcag gggggtctga aataattcgt tttgcaaaat
    63241 gtatctgggc ctaactgata tttctattgc ttattctttt atatacaaaa tttaagaata
    63301 caaaccagat aaccaataaa aaaggaataa agcgtgtcgt gctttcgaat aacaagctga
    63361 aagcaattga agtaaaaaac accaacaaag caagatcttt atatctttgt gtagtgttcc
    63421 gtttcttttc tttaataaat tgaacatgtg cacacaagat cgaagaccac acaaatagaa
    63481 aacaactcgt tgtcatgcct gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa
    63541 aatgtagaca aagaccggca aaaaataaca ttcctgtaat taaaataccg tgtaaaggta
    63601 cgcctttctt agaaagcgca caaaaccaat ggggcaaact tttttctttt gctgtttgat
    63661 ataaaatcct gctagtactg tagactgcac tatttgctga ggacaaagaa gcaaccaaaa
    63721 tcattagatt gacaattgct gctccgcctt ggcaatggat cacttgcagc atagtcacta
    63781 aaggactttc ttttaaatct aaacaatgcc atggaacaac aaataaaatc gacaaaatcg
    63841 ttcctatata gaaaaagaga atacgccaag gcagacagtt gattgctttt ttcaaattct
    63901 tttgcggatc acttgtctcg ccaacggtta agccgatcat ttcaattcca gcaaaactga
    63961 ataaaaccat aggaaatgag actaccattc cagcaaagcc aaacggtagt aattcatcga
    64021 tctgtccaaa attttctttg attccgatag actgggtatg aaaatctgag acactcaatc
    64081 ctatacccaa taagataaaa aggaaaatcc ccaagatctt gatgattgag aaaaaagatt
    64141 ccaattcacc aaaccacttg actgcaacaa ggttgactgc cattaagatc attagtgcgc
    64201 tacctccgct tatcaaagct ggtacatgta caaaccagaa atggatatac aatcctattg
    64261 ctgttaattc cgcaagtcca gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa
    64321 atgcccactt tttgcctaaa taatagtcaa cgaaatcact gatagagtta aatgtactat
    64381 ttgctaaaaa taaatcaccc atggcccgca tcaaaccata aatagccaaa ccgcatatca
    64441 aataaacgag aataacagct ggccctgctg atgcaattgc ctttgctgaa cctaaaaata
    64501 atcctgttcc aattgcgcca cctaatgcga ttaactggat atttcgatta gttagagtcc
    64561 gtttcattgc cggtttttcc atcaagttca cctttttctt tttcaagcag catggcgact
    64621 tcctgttccc aagaaggttt ctttattttt tcaatacaga gtggtactac aaacaaaatg
    64681 acactgatgg ctgtgactgc aattagattg atccatgttc cagctttcat cgtcagtccg
    64741 acagaaaatc cgattgccgc aaataaaaca ataacgacag tccaaactcc aagattgctc
    64801 tttttctctt tttttccaat tctaaatggt cgattcaact ttgcatgtgt ataacgcaat
    64861 ctcaaaactg cgatccccat gatgacatag acgatacagt agataacagt agttgaattt
    64921 acgagcatca aaaatgcttg attgacgcca ggaatcagca aataaaaaag tgcaaagatt
    64981 gagataatga cagactgagt caaaataacg gatttggata cgtcaaattg attcgtcttc
    65041 caaaaattga attttggtgg atacgcacct tttctagcac tttctgtcac tgtctttgct
    65101 ggtcctgagg cccaagcaga taattggact gctacaccaa tgaagacaag taaactaaac
    65161 agattcacaa taagatgtgg caaaccaaga atttggcaat aaatcgaaat agattgcgcg
    65221 atattattca attccatttg tccttttggt acgacatttg ctacaactaa agcattcaaa
    65281 atattgacga aaaatataaa aattaacgcg gcaaatactc cgcgaatata cgttttaact
    65341 ggattttcca atcgagtgat atacacagag gacatttcga taccagtaaa aataaacatg
    65401 atcggcgcaa aataaacaaa tgaggatgca gtcgtagtat ctggtattaa tttgtcccat
    65461 gaaaaactgc ctaatgttcc ggttggtacg atcccaactt tgatccacgc agcaaaccct
    65521 aataagagca tcatcgtcaa aggcacgtac aatcctaacc aaacaccgac ttttccgccg
    65581 atttttgcca tatcaaattt caaattcaaa agagtgatga tccaataaac gactaaaatg
    65641 acaactaatg taaatttgct gttcaaacca agcggcacat tatcgataat attaccaaac
    65701 aacggagcga gtgctgaagc gaccatcacc atcccaggaa acatttggac ccataacagc
    65761 catgagacaa cgaaccccca tttttttcct agagcattcg atacccaaag ttctggacca
    65821 cctttttggg gaaacattcc agcaaattca ccagagatca atgagattgg aaaagcgtac
    65881 aataatactg caaccagcat gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga
    65941 atatttcgta cacttgctac aagggcgcag gtcattccga taaaggtcat ggtcgtgatt
    66001 tgctttgatg aaccagtatc tttcatcttt attccttctt tcaatagcga catatgtttt
    66061 tgttttcttt tatttttgtt caagaacaga aatgacaagg tttcatcgat tcagtacaaa
    66121 acccagtatc accgaaacat ccattgaacg atcttagtga actgggtttg atacatgaaa
    66181 cgaacgatcc aacttataga acgttttata aaaaagaata tcttggcgag gaatatatga
    66241 aaaacagggg gaaatacaat tgttttgatg aagcaaataa ttgtctgttc tccatatgtc
    66301 tcttgttatt ctttcacaca aaaatcagtt ctattggatg atttcgttta gtttcttttc
    66361 cattgctttg gcgatttttt ttgtataaaa attgaaaatt cgatcatcat tcaaataagg
    66421 agtcatgata gccgcacgta gcagagtgat tttctgttct tttttccatt cttctttcgt
    66481 tagacccatt ctttcaacaa aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt
    66541 agtatgagaa gtaataaacc gttcaccata aacatcgcca tccatataag aagaccgatc
    66601 gaacattttt tcatttaatt cattgatagc gttcaaatct gtacagcctt gttctttgaa
    66661 tacccaattg accatattaa aatcaggatg attcaatgga tagacttcga ttgtttttcc
    66721 tttaacagta aaagtcagat gatcgagaaa ttctctgaat ctttgagctg cctcgattga
    66781 agccccaatc aattgaccat atccggtcac attcaatggc aatacacgat gtgctgtcca
    66841 gactgcggcg gcggttgctc cagcttttga tccttccaaa atataagctc ctaacatatc
    66901 tggagccttt actgattttt caaacacata tggcgcgaaa taagaaatga tatttctcat
    66961 atttttatgt ttgattacga ttcctccagc agcataaggg acatagccca tcttatgagg
    67021 atctatcgtg acagaatccg cttctgaaat agctctgaac ccttcatata cctctttgtc
    67081 aatcgttacg cagtggtgga aaacatgatg ttcttcaaag aattcagcta aggaagcata
    67141 cggcacaaat tctcctgctt cgtttagaaa cagtgaacgc gcatagccgc catatgcggc
    67201 atcaacatgt aaatagaaat agatcccttc atcttttaac ctttctctta attggacgat
    67261 tttatctaca ctgtctactt gaccttcttc tgtcgttcct acgacagcaa ccacacctag
    67321 aattgggatt ttttgtccag ctaattcacg aattgttttt tccaaagcat taatatccat
    67381 tcgataatcc tcttgaactg ggatagcaac catctgatct aagccgactc cgcaaatatc
    67441 taacgccttc atccaagaat agtgttttgt ttgagggacc agccatttcc ctaatctttg
    67501 gatatttttt ccgctacggg aagaagctgc tttgacctca tccatttctt cgtccgtaaa
    67561 gctttcggtc atttccaaaa tttcttcaac agataaattc aaaagttccc actcagacat
    67621 ttttttcact ttttcaggca gcacttcctt gacagctaaa ggaatcgatt tgatgcaacg
    67681 ggcgtaccat aatccttcaa gattagcaat agaaccatct gctgcaatgt gtccccatcc
    67741 gtctgtcata ttgaataatg aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt
    67801 tgccatagaa gattccaatg caacattatt tgggttccac agcatcgcat agttgtaagc
    67861 taataaagca ggcatcaatg tttcagcatt catttgtccc cagtaacggc ctgctgaatg
    67921 ccatgggatc gatcctgcgc gtaatcgctg actgacttca tccaagacct ctatcatatg
    67981 gtcgcgggtg gctagataat ctggagtgag cttatctcct tcgctaattg caggcatatc
    68041 tgaaggaata taattttctc tccatccaag atgttcatct accatcttgt tcaacaacat
    68101 tttataaact ggtccatttt cagctttgtc gcctataaag acggccttga tatccatatc
    68161 tttcattttt cttccctcca tattttgttg gtcaatgtct tgcagataca tgtgtttatc
    68221 tgtaacacct cttcactata gcgccaaaaa tgagaaattt ttttttataa catcaaatcc
    68281 acgtagattt ttacaaacaa aaataaataa agtcggattt ttctgataat tcaacgcaaa
    68341 aaagcgaaca atttttcaat aaatcagtct tctaaaatag ataacctaaa aa
//
LOCUS       DAYYOA010000004        60462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000004 DAYYOA010000000
VERSION     DAYYOA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.004"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1535)
                     /locus_tag="ACHBKA_000235"
     CDS             complement(12..1535)
                     /locus_tag="ACHBKA_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9443960.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ACHBKA_000236"
     CDS             1787..2968
                     /locus_tag="ACHBKA_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9443961.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACHBKA_000237"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACHBKA_000237"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM9443962.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ACHBKA_000238"
     CDS             complement(5053..7764)
                     /locus_tag="ACHBKA_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9443963.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ACHBKA_000239"
     CDS             complement(7774..8475)
                     /locus_tag="ACHBKA_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9443964.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ACHBKA_000240"
     CDS             8661..9260
                     /locus_tag="ACHBKA_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9443965.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ACHBKA_000241"
     CDS             9341..10354
                     /locus_tag="ACHBKA_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM9443966.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ACHBKA_000242"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ACHBKA_000242"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM9443967.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ACHBKA_000243"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ACHBKA_000243"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM9443968.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ACHBKA_000244"
     CDS             11802..13175
                     /locus_tag="ACHBKA_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9443969.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ACHBKA_000245"
     CDS             13532..15676
                     /locus_tag="ACHBKA_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9443970.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ACHBKA_000246"
     CDS             complement(15728..16768)
                     /locus_tag="ACHBKA_000246"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9443971.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ACHBKA_000247"
     CDS             complement(17045..18847)
                     /locus_tag="ACHBKA_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9443972.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ACHBKA_000248"
     CDS             complement(18861..19613)
                     /locus_tag="ACHBKA_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9443973.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ACHBKA_000249"
     CDS             complement(19622..19984)
                     /locus_tag="ACHBKA_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9443974.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ACHBKA_000250"
     CDS             complement(20160..21032)
                     /locus_tag="ACHBKA_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9443975.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ACHBKA_000251"
     CDS             complement(21058..21570)
                     /locus_tag="ACHBKA_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9443976.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ACHBKA_000252"
     CDS             complement(21708..22499)
                     /locus_tag="ACHBKA_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM9443977.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ACHBKA_000253"
     CDS             complement(22554..23162)
                     /locus_tag="ACHBKA_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM9443978.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ACHBKA_000254"
     CDS             complement(23155..23472)
                     /locus_tag="ACHBKA_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9443979.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ACHBKA_000255"
     CDS             complement(23628..24356)
                     /locus_tag="ACHBKA_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM9443980.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ACHBKA_000256"
     CDS             24515..25396
                     /locus_tag="ACHBKA_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9443981.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ACHBKA_000257"
     CDS             complement(25633..26676)
                     /locus_tag="ACHBKA_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9443982.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ACHBKA_000258"
     CDS             complement(26679..27347)
                     /locus_tag="ACHBKA_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9443983.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ACHBKA_000259"
     CDS             27489..29825
                     /locus_tag="ACHBKA_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM9443984.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ACHBKA_000260"
     CDS             complement(29940..30251)
                     /locus_tag="ACHBKA_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443985.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ACHBKA_000261"
     CDS             30432..31907
                     /locus_tag="ACHBKA_000261"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM9443986.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ACHBKA_000262"
     CDS             31954..32538
                     /locus_tag="ACHBKA_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM9443987.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ACHBKA_000263"
     CDS             32540..32959
                     /locus_tag="ACHBKA_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM9443988.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ACHBKA_000264"
     CDS             complement(33240..33524)
                     /locus_tag="ACHBKA_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM9443989.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ACHBKA_000265"
     CDS             complement(33876..34586)
                     /locus_tag="ACHBKA_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM9443990.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ACHBKA_000266"
     CDS             complement(34583..36055)
                     /locus_tag="ACHBKA_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9443991.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ACHBKA_000267"
     CDS             36193..36666
                     /locus_tag="ACHBKA_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9443992.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ACHBKA_000268"
     CDS             complement(36819..37370)
                     /locus_tag="ACHBKA_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9443993.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ACHBKA_000269"
     CDS             37475..38005
                     /locus_tag="ACHBKA_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM9443994.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ACHBKA_000270"
     CDS             complement(38488..38931)
                     /locus_tag="ACHBKA_000270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9443995.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ACHBKA_000271"
     CDS             complement(38947..40107)
                     /locus_tag="ACHBKA_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9443996.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ACHBKA_000272"
     CDS             40614..42314
                     /locus_tag="ACHBKA_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9443997.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ACHBKA_000273"
     CDS             complement(42416..43249)
                     /locus_tag="ACHBKA_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM9443998.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ACHBKA_000274"
     CDS             complement(43553..45718)
                     /locus_tag="ACHBKA_000274"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM9443999.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ACHBKA_000275"
     CDS             45978..48272
                     /locus_tag="ACHBKA_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM9444000.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ACHBKA_000276"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ACHBKA_000276"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9444001.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ACHBKA_000277"
     CDS             48923..49930
                     /locus_tag="ACHBKA_000277"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9444002.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ACHBKA_000278"
     CDS             49952..50971
                     /locus_tag="ACHBKA_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9444003.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACHBKA_000279"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACHBKA_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9444004.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACHBKA_000280"
     CDS             complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACHBKA_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM9444005.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACHBKA_000281"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACHBKA_000281"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9444006.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACHBKA_000282"
     CDS             complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACHBKA_000282"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM9444007.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ACHBKA_000283"
     CDS             58397..59698
                     /locus_tag="ACHBKA_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9444008.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ACHBKA_000284"
     CDS             complement(59767..60366)
                     /locus_tag="ACHBKA_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9444009.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18242 a        12368 c        10834 g        19018 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgcaacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ct
//
LOCUS       DAYYOA010000005        55792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000005 DAYYOA010000000
VERSION     DAYYOA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.005"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            335..655
                     /locus_tag="ACHBKA_000285"
     CDS             335..655
                     /locus_tag="ACHBKA_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM9444010.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            809..1714
                     /locus_tag="ACHBKA_000286"
     CDS             809..1714
                     /locus_tag="ACHBKA_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444011.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1711..2484
                     /locus_tag="ACHBKA_000287"
     CDS             1711..2484
                     /locus_tag="ACHBKA_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9444012.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2665..4089)
                     /locus_tag="ACHBKA_000288"
     CDS             complement(2665..4089)
                     /locus_tag="ACHBKA_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9444013.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4300..4746
                     /gene="nrdI"
                     /locus_tag="ACHBKA_000289"
     CDS             4300..4746
                     /gene="nrdI"
                     /locus_tag="ACHBKA_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9444014.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4811..6793)
                     /locus_tag="ACHBKA_000290"
     CDS             complement(4811..6793)
                     /locus_tag="ACHBKA_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9444015.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6807..7703)
                     /gene="murQ"
                     /locus_tag="ACHBKA_000291"
     CDS             complement(6807..7703)
                     /gene="murQ"
                     /locus_tag="ACHBKA_000291"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9444016.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7706..8767)
                     /locus_tag="ACHBKA_000292"
     CDS             complement(7706..8767)
                     /locus_tag="ACHBKA_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9444017.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8978..9505)
                     /locus_tag="ACHBKA_000293"
     CDS             complement(8978..9505)
                     /locus_tag="ACHBKA_000293"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9444018.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9572..11074)
                     /gene="eat(A)"
                     /locus_tag="ACHBKA_000294"
     CDS             complement(9572..11074)
                     /gene="eat(A)"
                     /locus_tag="ACHBKA_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9444019.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11332..12453)
                     /locus_tag="ACHBKA_000295"
     CDS             complement(11332..12453)
                     /locus_tag="ACHBKA_000295"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9444020.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12579..13520
                     /locus_tag="ACHBKA_000296"
     CDS             12579..13520
                     /locus_tag="ACHBKA_000296"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9444021.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13670..14566)
                     /locus_tag="ACHBKA_000297"
     CDS             complement(13670..14566)
                     /locus_tag="ACHBKA_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9444022.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14835..16175
                     /locus_tag="ACHBKA_000298"
     CDS             14835..16175
                     /locus_tag="ACHBKA_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9444023.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16393..16836
                     /locus_tag="ACHBKA_000299"
     CDS             16393..16836
                     /locus_tag="ACHBKA_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM9444024.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17340..18758)
                     /gene="nhaC"
                     /locus_tag="ACHBKA_000300"
     CDS             complement(17340..18758)
                     /gene="nhaC"
                     /locus_tag="ACHBKA_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9444025.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19161..20384)
                     /locus_tag="ACHBKA_000301"
     CDS             complement(19161..20384)
                     /locus_tag="ACHBKA_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9444026.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20415..21254)
                     /locus_tag="ACHBKA_000302"
     CDS             complement(20415..21254)
                     /locus_tag="ACHBKA_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9444027.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21271..21951)
                     /locus_tag="ACHBKA_000303"
     CDS             complement(21271..21951)
                     /locus_tag="ACHBKA_000303"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9444028.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21953..23026)
                     /locus_tag="ACHBKA_000304"
     CDS             complement(21953..23026)
                     /locus_tag="ACHBKA_000304"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9444029.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23082..23320)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23363..23596)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23673..24344)
                     /locus_tag="ACHBKA_000305"
     CDS             complement(23673..24344)
                     /locus_tag="ACHBKA_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9444030.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24431..27079)
                     /gene="mgtA"
                     /locus_tag="ACHBKA_000306"
     CDS             complement(24431..27079)
                     /gene="mgtA"
                     /locus_tag="ACHBKA_000306"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM9444031.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27421..27595)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27768..28205
                     /gene="ndk"
                     /locus_tag="ACHBKA_000307"
     CDS             27768..28205
                     /gene="ndk"
                     /locus_tag="ACHBKA_000307"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM9444032.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28227..29093)
                     /locus_tag="ACHBKA_000308"
     CDS             complement(28227..29093)
                     /locus_tag="ACHBKA_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9444033.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29253..31346)
                     /locus_tag="ACHBKA_000309"
     CDS             complement(29253..31346)
                     /locus_tag="ACHBKA_000309"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9444034.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32095..32718)
                     /locus_tag="ACHBKA_000310"
     CDS             complement(32095..32718)
                     /locus_tag="ACHBKA_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444035.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32877..34274)
                     /locus_tag="ACHBKA_000311"
     CDS             complement(32877..34274)
                     /locus_tag="ACHBKA_000311"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9444036.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34306..35277)
                     /locus_tag="ACHBKA_000312"
     CDS             complement(34306..35277)
                     /locus_tag="ACHBKA_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9444037.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35651..36076
                     /locus_tag="ACHBKA_000313"
     CDS             35651..36076
                     /locus_tag="ACHBKA_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444038.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36564..37265)
                     /locus_tag="ACHBKA_000314"
     CDS             complement(36564..37265)
                     /locus_tag="ACHBKA_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9444039.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37281..37721)
                     /locus_tag="ACHBKA_000315"
     CDS             complement(37281..37721)
                     /locus_tag="ACHBKA_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9444040.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37721..38296)
                     /locus_tag="ACHBKA_000316"
     CDS             complement(37721..38296)
                     /locus_tag="ACHBKA_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM9444041.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            38537..38746
                     /locus_tag="ACHBKA_000317"
     CDS             38537..38746
                     /locus_tag="ACHBKA_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM9444042.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(39144..39656)
                     /locus_tag="ACHBKA_000318"
                     /pseudo
     CDS             complement(39144..39656)
                     /locus_tag="ACHBKA_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39782..40483)
                     /locus_tag="ACHBKA_000319"
     CDS             complement(39782..40483)
                     /locus_tag="ACHBKA_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9444043.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40591..42034)
                     /locus_tag="ACHBKA_000320"
                     /pseudo
     CDS             complement(40591..42034)
                     /locus_tag="ACHBKA_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(42048..43913)
                     /locus_tag="ACHBKA_000321"
     CDS             complement(42048..43913)
                     /locus_tag="ACHBKA_000321"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9444044.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(44034..44885)
                     /gene="licT"
                     /locus_tag="ACHBKA_000322"
     CDS             complement(44034..44885)
                     /gene="licT"
                     /locus_tag="ACHBKA_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM9444045.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45195..45908)
                     /locus_tag="ACHBKA_000323"
     CDS             complement(45195..45908)
                     /locus_tag="ACHBKA_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM9444046.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45976..46302)
                     /locus_tag="ACHBKA_000324"
     CDS             complement(45976..46302)
                     /locus_tag="ACHBKA_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9444047.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46425..46652)
                     /locus_tag="ACHBKA_000325"
     CDS             complement(46425..46652)
                     /locus_tag="ACHBKA_000325"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9444048.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46974..48227)
                     /locus_tag="ACHBKA_000326"
     CDS             complement(46974..48227)
                     /locus_tag="ACHBKA_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9444049.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48288..49028
                     /locus_tag="ACHBKA_000327"
     CDS             48288..49028
                     /locus_tag="ACHBKA_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9444050.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49114..49872)
                     /locus_tag="ACHBKA_000328"
     CDS             complement(49114..49872)
                     /locus_tag="ACHBKA_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9444051.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49869..50333)
                     /locus_tag="ACHBKA_000329"
                     /pseudo
     CDS             complement(49869..50333)
                     /locus_tag="ACHBKA_000329"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(50352..50849)
                     /locus_tag="ACHBKA_000330"
     CDS             complement(50352..50849)
                     /locus_tag="ACHBKA_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM9444052.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(50917..54084)
                     /locus_tag="ACHBKA_000331"
     CDS             complement(50917..54084)
                     /locus_tag="ACHBKA_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM9444053.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54308..55192)
                     /locus_tag="ACHBKA_000332"
     CDS             complement(54308..55192)
                     /locus_tag="ACHBKA_000332"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9444054.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55287..55724)
                     /locus_tag="ACHBKA_000333"
     CDS             complement(55287..55724)
                     /locus_tag="ACHBKA_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444055.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        16033 a        11739 c         9608 g        18412 t
ORIGIN      
        1 tgtaaggtaa agcttttttt aatttattgc ccgataatat atagaaataa ggtctagtta
       61 ctgttttttt taaatgatta ataaataaca acttctttct atgaaattgc aaagcattca
      121 tcttcttttc cgctaacgat atatccgtca gtgcttggta cactttacta aaaagtatga
      181 gattgccgat atctggatag gtgtgctata ctacagaaaa agatatacaa tctgccaccg
      241 ggtaggataa agaggatgat cattccatta ggtctttgaa gggatgattt tcctcttttt
      301 gtgtattttt ttatagttaa ggaggaatgt aagtatggca tggatggaat taatcatagc
      361 aggaattttg gaagtattct ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat
      421 caattattct ttttacacag tgattggcat gattgctagt ttttactttt tgtcaaaggc
      481 aatcaaaagt ttgccgatga gtctctctta tcctatctgg acagggattg gcgcagttgg
      541 ttcggtcata attggtgtgg tcttgtttca tgataagctg aatctttcca cttggttctt
      601 tgtggggatg ctactcatca gtatcattgg catcaagatt actagcggcc actagacact
      661 tggaataagt actgatcaga aaaaataagc ctttttattt aatattcaat ttttttatga
      721 aaaaataaga aataagtcca atgacctagc gacttctcca ctcatcaggt gtatgcttgt
      781 actatctata gaaaaggagt gaatattttt gaaaagtctt tcggtccaag aattatccaa
      841 gaactttggt cacaaacaag ccctcagcga tgtcagtttt agcttatccc ctggcaaaat
      901 cgttggatta gtaggtccta atggcgctgg aaaaacaacg atcatgaaag ctattttagg
      961 actgatccat tactcagatg gaacaatctg tattggtgaa aatgagattt ctccctctag
     1021 tcataaaggg ttggaacaag taggagcatt gatcgaatac ccgggtatct atccattctt
     1081 aacaggctat gatcatctga agttattctc tgaaaccaac gatgtatctg ctatcgacac
     1141 tatcgtgaat cagctcaaga tggaaaagta cattcacaaa aaagcgaaat cctactcgtt
     1201 gggaatgaaa caaaaattgg gaattgctct tgccttactg aaccatccgt ctatcgttat
     1261 tttagatgag ccgatgaacg gtttggatcc acaagcaaca agagatgtgc gaaaaatcat
     1321 taccgacttg gctactcagg gcacctcttt cttgatctcc agtcacttac tcagcgagct
     1381 tgaaaaaatg gtcgatgagc tgatcctgat caatcaagga aagatcatca aacaatgtac
     1441 aatggctgaa ttgaaccaaa cagcgcaaga tttcatcgtt atctctacaa caaatgatct
     1501 agctgctcga acgatcttga cagaagctgg atatactttg gaagatcctg ataaaatcaa
     1561 gcttttaaaa gaaaacgaag aattattagg aaccgtgatc cagctattgt ctgcacaaaa
     1621 tatccatgtg acagacgtcc agcatctgac gaatgatttg gaatcttctc tattggaaat
     1681 tctggattca gtaaaggggg aagaagaaaa atgacattgc ttgtaaaaca agaattattt
     1741 aaattgatca agaaaaagtc aactgctgtt ttatctgttc tattagttgt tttgctcata
     1801 ggaacagcac ttctagccaa aaaatacacg acgatcattg atccagttga aatgactgct
     1861 cagcttttta gtgccacatc ttggattgtc tttatcatga ttgcagctgc aagcacgatc
     1921 atttcgatgg aagcacaata tggtacattg aaaaatctcc tttatcgaaa gtattctcga
     1981 ggagaaatat tagtaagcaa atggatcaca ttagttattt attctgttta tttatacctt
     2041 ttagctatta ttgtcactgt gttgatgaaa ctgattcttt tcccttcgat ttccttcacg
     2101 gagaaggttt ctactggtca aacactgatc caaagcttgt tcacttatac tttgggtagt
     2161 tatatcggtt tatggctgat tttaagttta gtgttgatga ttgcttgctt tatcaatagt
     2221 tccggcgcat ccatctcagc aggaatcgtc ttttattttg cttcgtcaat catatctggt
     2281 atccttattg cactcatcca aaaatgggag tggatcaaat ggaatccgat cagtatgttg
     2341 aatctgcaaa accaaattgg aaacgaagag atcatgaagc aattgactca tctttctact
     2401 aatcaaatgc tattcggtaa tttagcttac atcgttttgt tcttagcatt aggatatctc
     2461 gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag aggctgtgaa agagacgttc
     2521 agccttattg ccgattggct gtctcttaaa caccgtttat tctgaaaaag gagctgtgac
     2581 aacatttgtg tcacagctcc tttcctagta tgtttttgaa taaacgacag gctcacaacc
     2641 tctctttata cttcttttat acttctatac gtgaagcatc ccagtggcaa ataaaaccat
     2701 tgcaatcact ccgaccatcg tcacacctat aagtaaaatc ttatctaagt ggatatgttg
     2761 tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag
     2821 aacaagaaca agcattactt cattaaaggc tacgactagc atagcccata cttgaaaggc
     2881 acttgctaaa agccctacta aaaattggac ccattctttc cgctgataag aaagtttcat
     2941 ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc
     3001 aaagttataa gcactgtcag tgaataataa tgaaagtaaa aagacattcg tacataacgt
     3061 cgtcaatacg atagcataag ttggtgcccc atttttgttg agttttcccc atgctttagg
     3121 caaaagtccg tcttcagcca tgtttcccat tgtttcaatc ggtaagatcg tccatgagag
     3181 ccaaacacca actaaagaaa tgatcaaacc gccgttgatc acgactgctc cccatttacc
     3241 aaccatcatt tcaaacacat agcccatagc aggctgcgcc ttgatcgttt gcagctgatt
     3301 catcgtcatt gtcccatatg gaagcatgga aaccaagacg taaataataa tcaagccgaa
     3361 tatccctaga atcgttgctt tcccaacatc tgattgtttt tttgctcgtg aacctacgac
     3421 agaggctcct tcaataccaa caaataccca aagcatcacc atgatgcaac tcaatacttg
     3481 actggaaaac gggatcggct gtccattcgc attcaatata atgtcgctag aaaagttatc
     3541 aatgaattgt tggatcgaaa acgtgctgat agctactaca ataaataaaa ataatggcac
     3601 taatttacag aatgtgacga tagcattgac gaaactagca ttttcgactc cccgattcac
     3661 tagccatgca tatccccata aaaagactgt actcatcacg atcgatgcca gattttgccc
     3721 accttcaaat actgggaaga aatatcctaa tgcgctcatc aacaatgttg caaaagctac
     3781 gttgcctaac cagcatgtga gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc
     3841 aaaaccttct tctgcgtaac taaagatccc gccttttaaa tctggacgtt ttttattgat
     3901 attattgatc gacaaggaga gcatcaacat tgctgttcct acgagtaccc aactgaaaat
     3961 agctgggccg ccagccgatc cggagatgtc cgtcataata ccaaatacgc cacctccaat
     4021 cgctgaacca attactaagg cagcaagtgg aattaaacca attccttttt tctgttgctc
     4081 tgtatccatg taagcatcct ccaaaataaa accgctttta tcaaaaagta taacaaacct
     4141 tttttatttt tacttaatta ataaaaactt taatagaaat ataattctac cggctttttt
     4201 ctcattatac aagcaaagcg cctagtccct ttccaaatta caccaccaaa aaacttaaaa
     4261 aagatgctat actaaaagga aagaattgga ggatgatcca tgaacatttt atatatatcg
     4321 atttcaggaa atacgcgtgc ctttgccaaa catctagcag aatatgctga aaaaatgcat
     4381 gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt
     4441 acgaaagaaa cagaaccatt ttttactttc gttcctactt atttagacgg cggaaatggt
     4501 ttagataacg gtgatacaga aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa
     4561 gacaatcata aactttgtct tggtgtagtc ggtagcggaa ataaaaactt caataatcag
     4621 tattgtctaa cagccaaaca atatgcacaa acatttggct ttcctttctt ggcagattat
     4681 gaattacggg gaacaccatc tgatgtgaca cgaatttatc agattttagc agaaaacaat
     4741 aaataataaa aaagaagaag ctgaaaaata gatttcagtt tcttctttcg tttctattgt
     4801 ttctatcata ttaagctttg acatccatca caggtgcttt tgcttttgct ttacctgttt
     4861 tttccaagac aaagtttttt acacgatcca tattggttac caagacgatc atggatgttt
     4921 ctttgcctgc tgctttgatc gcttctaagt cagcttctgc tacaagcgta tcaggtgtca
     4981 cttgctgccc ttctgttact ttcacatcaa atggcgtacc gcctaagtca actgtgttga
     5041 ttcccatatg aagcaagatt tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg
     5101 ttgggaagac agagctgact accccagtaa ctggagaata gatatgtccg ttttctggtt
     5161 ctacaaagta gccttcaccc atcatctttt gtgcaaaaac tggatcactt gctttatcta
     5221 aagaaacaac tgttccgtca gcaactgatg agaggatagt cacactttca gctgaagttt
     5281 gaggtactac tttttcaggc acttcattta ctgtggctgt ttcagtcgct aattcttctt
     5341 tttctaaacg gtctttagga ataccgaaga agaatgtagc aacgaagcca ccagcataag
     5401 ctgccaataa tccaaggatg tagcctaacc atttaccgtc agagatcaat gggatcaaag
     5461 cggctcctga aggtccaata gcgatagccc cgacatttcc gatcattccg atcactgccc
     5521 cgccgatacc tccaccgata caagcagtaa taaatgggcg tcctagaggt aatgttactc
     5581 cgtaaatcaa tggttcgccg attcctagga tcccgactgg taaagctcct ttgatcattt
     5641 ctgttaattc tttatctttt ttgcagcgaa tccataatgc caatgctgca cccacttgtc
     5701 ctgcaccagc catcgcaaga attggaagca atagcgtacg accagtttca tcaatcattt
     5761 gcacatggat tggagtcaaa atttgatgca gtccgaacat aaccatcggc aagaataatg
     5821 tgcctagaac gaagccggca aatgctccac caacgccaag tacccagttg atcccaccga
     5881 ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa gaaaatttca atcagaccga
     5941 tggcaatcaa tgaaagagtt ggtgtaacga tgatatctac cgcatcaggt actactttat
     6001 gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa gatgacacct aggatcccac
     6061 cttgtccagc tgcaagagct tcaccagtaa ataagttttt cagtggtgct tccggattca
     6121 tccctgtcag catcacaacg gccccgataa ctccaccaag agttggtgtt gcaccaaaaa
     6181 cttgtgcagc attgatacct gtatagataa ctaggtagct aaacatcccg tttttcaaga
     6241 tgttcatcac atcgatatat tgttgccatg ttgctgcatc aagtgttccg gctgtgacta
     6301 agtttgctaa aactgcggca atccctgcca cgatcccagt accgacaaat gcaggaatca
     6361 ttggcacaaa gatattagaa atgtctttta ataccgattt gattttagat ggtttttgtt
     6421 tcgatttttg tgccgctttc atttcttgtg cccgttcgtt tacacggtct tttccagatg
     6481 ctgatgaatt tgctgtagct gtacttacag aagaaagttt ttctcctaat ttcacgcctg
     6541 ccaaatcaac catttgctgt gctactttgt tcactttccc aggtccgatg atgacttgaa
     6601 gctgttcatc ttctactaca ccaagcactc ctggaatcgc ttttaattca gagacattta
     6661 caagatcatc attgatgatc ttcatacgca cacgagtcat gcagtggaca atgctttcta
     6721 cattgcccat gccgcctgcc cctttataaa tttgttttgc cagttcttcc aaagtcagtt
     6781 cgttttttgc catggtcttt tccccctcat aaggtccctt ttacaaatcc gtttgcttca
     6841 gctaattttg cttgagcttc ctctttatca ctatttgtca aaatcatgac gatagccagc
     6901 ttaacatttt gatctgcttc ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc
     6961 gcttgcatga tgattcgttt tgagcgttcg attagttttt catttgttgg tttgacatcg
     7021 accatcaagt tattgaatac tttacctata ccaatcatac tgatagtaga aatcatattc
     7081 aagataagtt tttgtgctgt tcctgacttc aatcgagtag agcctgtcaa aaattctgga
     7141 cctgcatcga cttcgattgg gaaatccgcg tgtttactga tttcggcttg tttattgcaa
     7201 gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat ctaatccacc gatcacatat
     7261 ggtgtacgac cgctagctgc gataccaata actatatcat tttttgtcaa attcagcttg
     7321 attagatctt cttgccctag ctcataagaa tcttcggccc cttcgacagc tacagtcatt
     7381 gcttcttctc caccagcaat tagacctatg accatttccg gttctacacc gaatgtcggt
     7441 acgcattcag cagcatctaa cacacctagt cttccacttg ttccggctcc catataaatc
     7501 agacgtccac cttttttaaa ggactcaatc gtgttagcaa tcactggttc gatacttgtc
     7561 agttcttttt caaccgcagc ggccactttc tgatcttctt tgttcattag ttgaacggct
     7621 tgagaaatac tcatctcatc taatccaaaa gtttcttggt ttctgcgttc cgttgttagg
     7681 ttctctaaat tgattttgct catttttatc ttccttcttt tcttataaat tgatagttca
     7741 taccagcttt gatttggttg atcaatggta gctcttcttt gataacttta gctactacat
     7801 tcactttttc atccgctggc aaatcatatt tggttaattg gatctcaccc atatagcgca
     7861 aatacttttc gttatctaaa gtaatagacc ctttcatacg cgtagctgta tttcttgctg
     7921 ggataggcgg aatctctcga aaacgtgcat ctacgcttcg tatgacatct cttgcatcgt
     7981 cttgccgatt cgtatgcttc cctaatatat attcgtaaaa ttcttcatct acaggttccg
     8041 tatggagcaa gactgtctgt tctttttgga aagaagcaaa ttgctcctga acgttttctg
     8101 acagacctgc atctccgatg taaacaatat ccgtgttact tgctaataga tctagagcgc
     8161 ctgcaagagg atggatccct cgatgttctt ccaaagttgg caatccttga tatagtgggc
     8221 ctctcaactc tgtatctcct ggtacaaaac cttggacaag gagaccttga attttgagcc
     8281 attgattttt tttctgatac cattctttgt ccagccctgt ttcgggacgt ggataataat
     8341 tatgccaagc ttcaatctgt gagaaatcag cttctgcttc tttcaattca tcgatatctt
     8401 taggcgtgat cgtgctagca ttcaaactga ttttcaattg atgtgaccag tcggcaatct
     8461 gctgattgga aatatgataa tccattctta aacctgttac accaattgct ttcaaaggcg
     8521 ttagatcttc taatgaaaaa cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca
     8581 attctagttt attctttttc gcttcgcttc ctagttcgat caatcgcttt tgatatgccg
     8641 aaacatcatc ttctggtata tgcatggaag tgaaaatacc gacaaagcca ttggatgcca
     8701 tttggcggat atactgtttc ttctcttctg acaaagaatc gttcataaat attgaaaagc
     8761 caaacatatt atccctcttt ttctgtatcc gtcttcattt ctatgactag agtatatatc
     8821 taaagaaaca aaatttcaat acttttatta aaataattaa atttcatttc taaaggttga
     8881 gtttatatta tcaaaagaat tgtcataacc taaaaaaggc actgctaagc aaaagcagtg
     8941 ccttgaaata ttttatttaa ataacacaaa aacataatta ctcataagaa accaattctg
     9001 tattatctac catgaatgta aagaacccct cattaggcat cggatgaaaa taagcattga
     9061 tcactcttat caaggcatta tggcaaacta acagatagac attttcttcg tcttcgatgc
     9121 attctttcaa taaaggaacg atccgtgcat acacatctaa gactgactcc ccatttggga
     9181 atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc atctttgctt ggcagtccat
     9241 cataatcccc aaaatccatt tctaccaatc gttcatcaac ggtcagcggc aagctgagac
     9301 gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag aggggaatgg atgattttcg
     9361 tgattggaac aggcaaatct actacctttt ctgctagttt ctgtgcctgc atctgtcctt
     9421 tttcagtcag cggcacatct gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt
     9481 ctccgtgtct cgttacataa agcatgtgat tctctccttc gttggcaaag ttccccttaa
     9541 ccataccttg ttgttcttga gattacaagg gttagattct ctcaagagaa ataatctcag
     9601 tagatacttt atccaggaag gtttgatcat gttccactag taacatggca ggtttcacgt
     9661 tcaagatcag ttgttctaat tgttcttgat tgaagacatc caaataattc aatggttcat
     9721 cccatgtata tagttcagct ggctgtgata aagatttagc caattccact tttttccgtt
     9781 ggcccatact catctgctcg atcttgttat gaaaaacatc tctttccatc ccaagctttc
     9841 ggaggttgtt caaaaatgct tggtagtcta cttggttttt ctctgcaaat tccgccaacg
     9901 ttcctcgatt gtcttcataa ttttggcttg cataactaat gctcagatgt tttggctggt
     9961 atttttctcc tataaccttg ccgttgaatg cccccagaag ataatggata atggatgact
    10021 ttcctgcacc gttaggacct gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa
    10081 ttggctcgaa taacagattt tcatacccta attgaagatc ttctacgctt aaaagtcgct
    10141 tatggtgaga cgcttggcta ttcatcgtca aagaatcgat atattcgata tcttttaata
    10201 gtttttcttt ctcgctgatc tgggtctcca tccgattcac gattgctttg gatcgtttca
    10261 tcgtccgtgc agcatcagca cccactgctc ctttattcac tcgtctagat tcagtatcga
    10321 tgaatccgac ttgtttcttt gttttatctc cttctcggga acgagaccat tcggcttttt
    10381 cagctgctgt tttctttagc ctactgactt cttttttcaa tttttcattt tgagccattt
    10441 caaattcatc acgaagtttt ttctgttctt catagataga gaaattccct tgataaagtt
    10501 ccagttgact tttttcgatt gctaaaacat ggtccactac ttcatcgata aatccccggt
    10561 catggctgac cacgatgaag ccttgttttt tctttttcaa ataagccgct acttgttttc
    10621 taccagagat atccaaatga ttcgttggtt catcgattaa cgggaaatga gtgtcatcca
    10681 caaataaaag tgccagtagg acttttgtct tctctccccc cgatagtgtg ctgaattctc
    10741 tccataagat ttcaggatct gtttgcatca attggagctc tctttcgatt tcccatatct
    10801 caaaatccgt aatatcattt aacacgtaat aggttaaacg ttctttatct tttgtctgtt
    10861 gcgggaaata ggcaaattct tgctgatgga ttacttgccc ttgataaggt agtttgtttt
    10921 gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg tccgataagt cctagtttcc
    10981 attgtgtgtc aaaatttaga tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc
    11041 cgaatgtcag attttttatt tcgattttag acattttcca tcacctcgta aaaaaagacc
    11101 agtcctattt tcagaactgg ctaaagagga tgcaagggtt ggatacacac aaaaagaccc
    11161 atacatcgaa aaaaccaagc caatcccgaa aatctgcaca ctgcttcctt gtctccacaa
    11221 ggtacagtct gaatgcagct ttcatcaatc gacttaattt ttcaatgcac ggatccctca
    11281 ctttgtccta tatttgaaat tacgatagca taaagaaaat gattttgtca actacttcgg
    11341 gaggatggtg aaaaatctgc taagtgtctg tcctaagatt tttgtttttt ctaatgtttg
    11401 cctgtgttcc attgcttctt ctaaacctat tggacccaaa tgaatactta agacaattgg
    11461 gagaaaatca gaaagtcctt ctagctcttg gtctctactt ccgcaaatag cgattattgg
    11521 ttttcgatat ttttttgcaa gcactgctac accgtatgga acttttccgt ggatcgtttg
    11581 ctgatcgatc cgcccttctc ctgtaatgat gacatctgct tgttcgatct ttttttccaa
    11641 tccagtgatt tggctgacta aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag
    11701 ggctccacca ataccgcctg ctgctccact tcctggaata tcatctaaat ccacctccca
    11761 gaaatctttt atttgctgag caatctttcc tgcttgttta tctaagaaag ctagcgtcgt
    11821 atctattcct cctttttgtc ttccaaattg ccaagcagct ccttgttgac ctgtatatgg
    11881 attggtaaca tctgcagcga taatcaattt tactttagaa acaagaacgt ttcttcgatc
    11941 aaaatgaagt tttttagtag ataataaagg atttccttct gtgtaccctt ctaaagaagc
    12001 acccaaggat tggagtaatc ctagaccacc atcgctacac ccgcttcctc ctaaagtgac
    12061 aataatttgt tctacttctt gaagcagtgc atctttaata agttctccta aggcataact
    12121 ggaagcaagt cggatagttt gatccgttgg tttatcaagt agatctaacc ccaatgcttg
    12181 tgcagattcg atgactgcgg tgttttttcc ttgccaagtt gtcaataaat agctgatttt
    12241 tcgctgacgg aataagagat ccatcccatc aatggtacgc catgttcccc tgagcgcgta
    12301 atagattgct tctgcagtat tttctccgcc atctgcaatc ggcacattga tttttcttcc
    12361 tttacgatct tcccagccat ttagaactgc ctgatttagt tcaaatgaag aagcacttcc
    12421 tttaaatgaa tcaattgccg ccaaaatatc catctgtatc cctcactttc ttcttaagta
    12481 agacactttt tttcattttt ttcaaacaaa acaatgctat tttcttccat ttttctatat
    12541 actaaaaggg aactcattga ataaaggaga gaaaaaacat gaaaatcgca atagctgggg
    12601 ccggagcaat gggcagccga atcggattga tgctacatca aagcggaaat gaggtcctct
    12661 tgatcgatcg ttggcctgct cacattgaag ccattcgtac aaatgggttg attgctgact
    12721 ttaacggtaa agaagtggtc gcaaaactgc caatctattc accagaagaa atcatcgaaa
    12781 gcaacgaaca cgtggacttg atcgtagcat taacaaaagc caatcaatta gatgatatgt
    12841 tttgttcgat ccaatccatt attactgaca atacttatgt tttgtgttta ctaaatggat
    12901 taggtcatga agatgtactg gaaaaatatg tgccaaagaa aaatatctta tttgggatca
    12961 cgatgtggac agctggtcta gctggtcccg gaaaagtcac attgttaggt gatggagaaa
    13021 tcgaactaga gaacttggaa ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct
    13081 ttcaagaagc aaatttaaat ccgatctata gccataacgt tcgttattcc atttggcgaa
    13141 aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat tttggattgt aatattgctg
    13201 aactaggcgc gcaaaaagcg gcagagagca tggttcgaac aatcgtatct gaatttgctt
    13261 ctatcgcagc aaaagaagga atcatactag atcaagaaga agtgtaccaa catattgctt
    13321 ctacttatga tcctgacaac atcgggttgc attacccatc tatgtaccaa gacctgatca
    13381 aaaaccatcg tttgactgaa atcgactaca tcaatggtgc catttggcga aaaggacaaa
    13441 aatatgatat tgctacacca tattgtgcct ttttgaccca attagtccat gcaaaagaag
    13501 gaatccttgg cgcagaataa aaggctctct gatgagaatc agctgaatca gcagctggtt
    13561 ctcttttttt aagcccaata aaagatcaaa aagaaaacac aactctcatg atgccataag
    13621 aattgtgctt gtttgtttca gtacataatc tcttcaaaat gtaggttttt tatttgtttt
    13681 caacagagga gaggtcatat tcgtattgct cataaatagt ggaatcgtta gtgataaatc
    13741 gttttgccca attttctgca tcataaaatt ccaatagctc tgataggatt ggcttaaatg
    13801 gtgtatgcag aaattccggt aattcatcta aatcacgttc tcgaagcaaa aaaatatttt
    13861 caggacgata aaaatgataa tgagcaatcg cttgattatc cgtcactagt ttcttttgaa
    13921 aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg accctcccgc aacagttcca
    13981 gaatcccgcg cgattcccct gtttttttca ggtactcttc ataagtaaga tatccaggtt
    14041 gttcattccc tcgatgtgga agaatgaaat agttggtcgt aacatctctt tttataaatt
    14101 cctgcttata gctttctgct cgttcttttc tcccattatt cgttgcacca aaaaatagat
    14161 ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga ataaaatgaa ctattatgtt
    14221 tcagcccaag ttcttttgct tccaaaaaat caaaatgata aatctcatcg attgccggtt
    14281 catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt gaagtgattc caaaaaaata
    14341 acacaatttt tgcattggta aatgggcgca cgcgtcgagc catctccata tcaaatccat
    14401 aatctaaaaa aacgatcagc tgataatttg ccaactgttg tctagctgat ttaatgaatt
    14461 ccgactgtag taaatctttt ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat
    14521 aaagtgattt attttttgca actagcagta cgtcattttt gttcatcttt tccgcctcac
    14581 tttctctgtc actttttcct atgatagcct gtttaaatag taaaaagcca gtaaaacgtt
    14641 tcctatattt gtatgaaaat ttaacatttg tatgaaaata tgatgaaata aatcattctt
    14701 ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat tgtctgaaaa tttgttatca
    14761 ttagtcattg ttaaatattg ctatttattc ttaatataaa ttttgattgt gaatttaaaa
    14821 gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc agacatatca
    14881 ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt ggcgaagcga
    14941 ttgggatcgc cggcccttct gtattgattg cttttttgat tggtgggctg attttatata
    15001 tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat cacggattat
    15061 ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat tgggtctact
    15121 ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc tttttgcagt
    15181 tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt ttaactacga
    15241 tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg gcttttgcta
    15301 aaatcagtgt cattatctta ctgatcattt ttgctactta tcttgtcggt cagcaaatgt
    15361 tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg tttactcctc
    15421 atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac ggcggttctg
    15481 aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa
    15541 tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt ttgcttttga
    15601 ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg atggtatttg
    15661 aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgtgattgtc cttgctttgt
    15721 tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg
    15781 atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg
    15841 ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat tttgtcggtg
    15901 acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg atctggatca
    15961 ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg gataaaggaa
    16021 tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg ttcacgaact
    16081 ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc agttatcaaa
    16141 aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt ttattttatt
    16201 tatgtggaat agatggagct attgaagaaa ggagttgtga caaaagtctt gttgcaactc
    16261 ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg
    16321 caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat gaaaaaaagg
    16381 agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt ttcaacggta
    16441 gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt
    16501 acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat gctgttgttc
    16561 atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc tagtcttctt
    16621 gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga cacagcttgg
    16681 atgaaggcca tgattcctca tcattctatc gctatcctgg caagtaaacg agctgattta
    16741 aaagatcctg aagtaaaaaa attagcggaa gaaatcattc aggcacaaga agaagagatc
    16801 gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca aataaaaaaa
    16861 gaggatgttc cagaagttat cagcttcaag aaataagaag aagctacttc tggaacgccg
    16921 tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
    16981 tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac
    17041 cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag tattagagag acaaatggaa
    17101 aaatagttcc tcatattttg ccattttttg agctaatacc gagggtcagc tgattgaaca
    17161 ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg
    17221 aaatggcttt ccacattttt gtataaattt ggcttaatac cgagaaactg gcttttgaat
    17281 accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt tttatgtttt
    17341 tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttttttgag
    17401 attccccaac ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa actgaaaaat
    17461 acgaatggca agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa acttccggca
    17521 attccccaag gcactaagta gttgattact gttccaccat cttctagcgt tctacctaat
    17581 acagttggat caaatcctgc tttttcataa gtttctttaa atgcattacc aggcagaata
    17641 acagatagga attgttcacc aataaaaata tttactccga tacttgtcat caatgttgta
    17701 aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc gttccatcac ggttgagatc
    17761 aagccaaatt cgatcaacaa tccacctaat gacaaagtta gaataatcaa tgacacagta
    17821 ggcatcatac tttcgattcc tccgcggcta agcagtgaat cgatttgttg gtttccagta
    17881 tttgaaacaa accctgattc gatgacccca gccaacttcg ttaaggaagt ttgtgggttt
    17941 tgaataaaaa tcattcctac tgctacagcg atattcaaca aaatcgttaa aattgccggc
    18001 acctttttcc atgcacacag taacattaaa aataaaggaa tcagtgacca gattgaaata
    18061 gtaaagtttg cttctagaac atcagtcact tcacgaatag atgaaagact tgcggcttta
    18121 cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg gacagttgac
    18181 cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctagattt
    18241 gttgattcgg ataatggaga catcttatct ccaaaaacgg cacctgaaac aatagctcca
    18301 acaaccagcg ctggatttaa gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc
    18361 gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca cacaacaaat
    18421 acagaaggga caaaccactg gacactgatc aatttgaacc caatgaccat tagagttgga
    18481 atgatccctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt
    18541 gggatgattc ctggtttgat gccattgatg attccttcgt gcagccaatc tgtcgggtat
    18601 ctttttatca ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt gatattaggt
    18661 gagaccttta atccgacaac gccaatcgca atgcctaaaa caataaccat taatacaagt
    18721 gttgcttcta aaaaacttgg tgttcctttt tttcccatga taatctctcc ttttttagtt
    18781 gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccgcaattc atccgcgatt
    18841 cctcctttat aaaaataaaa agacctgttt taaactaagg acgaatgact ccgcggtacc
    18901 accttaatta aaaacagatc tgtttttcac tttcaaactt ttattcaaat gtatggactt
    18961 tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa
    19021 aacacctaac cgctactgcg tgaaaagttg acttgtacga agtttacctt ccataagatc
    19081 atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc
    19141 acacacaagt ttatttttta ctagaaagta gcgccctctt gtttttttct tgctactatt
    19201 tcttgatgtt cttgcttgat tctttctaat agctttggct ctttcaacag atcaagagct
    19261 gtattggcga gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc acttttagca
    19321 gcagctttga attcttctgt gtgtccggca atatatgtct cagagattgc taccgtaggc
    19381 tggattgttg gtatgacttg gctgacattt cctacatcag aagaaccgag acttgttctt
    19441 tgttccgttt ctatttcttc atctggaaca cctgcttctt ttgcatgctg gaagaataag
    19501 tcatcaaaag aaggagtaac gatgacatca tcaactgcat tttggaacaa tccaaactca
    19561 aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat tttccacttt ttcatatact
    19621 ttatctaatg tttgccgatt tcctgcacgt aaatagaaac gtccagcagc gtaatctgga
    19681 atgacgttgg cagctactcc tccgtctgta atgatcccat ggatattgac atcttttggc
    19741 aattgcaagc gtagagcgtt gattccatta aacacttgga tcaatgcttc caaagcattt
    19801 actccttttt caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact
    19861 ggatcattcg ctaaagattc tgtcgttttc ccataacgaa aagctggatg gacgcataac
    19921 gctacatcga catcctcaaa aaaaccttct cgaacaaagc tgcctttagc tgatccgttc
    19981 tcgccccctt cttcccctgg ggtgccatat acacggattt ctccaccagt ttcatcaatg
    20041 aattgcttca ctatgctggc ggctaatacg gagtaagtac caaacaaatt atgcccgcat
    20101 gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa aggcaattgt gggaccgggt
    20161 ttattggaaa tataacgtgc atcaaaggct gttcggtgtc cagctacttc tttttctacc
    20221 caaaaccttt ctttttctaa ttgtttaatc aacgctgctg aggaataaaa ctcatagtta
    20281 cttacttcag gatgttcgtg gatatctaga gcaagtgctt gatattctgg taaacgatat
    20341 tggattagat ccttcaattg gtttgttgag attgttgttg tcataaaaaa ttcctcctat
    20401 gctagtcatt tttcttattt tctcccgaat gtttcccatg caggcacgct tgatcctttt
    20461 gatgttttct cgattacttt gactgtgtct tctgtttgat aagcgtctac tattttttga
    20521 tacgttttat tgttttcatc ttcttttctt gcaacgatga tattcacata aggacgtgca
    20581 tggtcatcca ctggttctaa gaagatggca tcagaagcag gattaaatcc agcatctaca
    20641 gctacaccgc tattgatcac tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa
    20701 gcatctaatt cagaaatgtc tagatttttt ctattctcgg taatatcaga aacagtgggc
    20761 gcttgtcctt ttgccttatc cactttgatc aatccagcag tttgcaataa cagaagagct
    20821 ctgccgccat ttgtaacatc attagggatt gctacttttg ctccgtcttt gatatcagct
    20881 acatccttca aagtgttaga ataaattccc agcggtgcat tgacagtatt tccgatcgat
    20941 actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa
    21001 ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt gattgtaatc tgtaaatttc
    21061 acgtattcca aattaatgtt ctcctttttc aaacggtctt tcacgttgtc ccatacatct
    21121 gtatcttctc caataatccc tagttttacg atagtttctt ttttgtcagt tgtatctgct
    21181 tttccttggc tacaccctgc caaccaaata attgctacaa atgtaatgac tacaaaacga
    21241 ataatttttt tcattttttc tcctccactt tcaatgtgta atttttttaa tcagttgatt
    21301 actgataaat tgacttaaaa aaactaagat tagaatcaat aatgtagcca caatcgttac
    21361 atctgtttgg aatcgattat accctctcga tatagcaaga ttccctaatc cgcctgcacc
    21421 tactgctcca gccatggtag tcagtccgat cacgttgatg atagtcaacg ccgagacccg
    21481 aatgatacca ggcaaacctt ctaacaaata cacacgtaaa atgataccga ttggactggt
    21541 tcccatcgct tctgccgctt cgatcacccc aggatcaact tctagcagtg cgttctcgat
    21601 ttgacgagca aaaaacggaa caactccaaa aatcaatggc actaaagctg ctttcgcacc
    21661 aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa agagaaagca agataatgaa
    21721 agggatggaa cgaatgatat tgatcagctt atcaagtatt tcaaatagca gacggttagc
    21781 tagtaatcca ttagggcgtg tcactactaa cgtcacacct aagatgattc caatcatccc
    21841 gccaatgaat gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt cgacaaattc
    21901 atccggaatc aaataaacat tgggcaaata tgttttaaag aaatcagtca ttttatcttc
    21961 ctccttcgat cactttgata cgactatcta aatcgttttt ttgtaaaata tttattttta
    22021 caccagattt gatcaaataa tccaatgctt ttttccgctg tttggtttct ccagctaaag
    22081 taacgatcaa actccctaac ggcgtttctt gtaataactc tacattgcca tataaaatat
    22141 tcgctgttac ttgatatttg ctgtacagat gagcgattag aggttcgttc gtctgatttc
    22201 caatataact taattccacc agccatttat tggcgatctg atcggcaaaa gcagaatggg
    22261 caatgatcgt ttctaatgct tggtcaagat gagtagctgt tcgaataaaa tctttggtca
    22321 attcttcttc aggattgctg aagatctgga tgctgctacc tttttcaacg attcttccat
    22381 cttccataac cgctaccttg ttgcagattt ctttgacaac ttgcatctca tgagtgatca
    22441 ggacgattgt taaacctaac tggcgattca atttttttaa caatgcaagg atctgaaggg
    22501 tcgttttcgg atcaagtgca ctggttgctt catcacataa taatacctta ggttcattcg
    22561 ccaaagctcg tgcgattgct actcgttgtt tttgtccccc cgacagttgt ttaggataag
    22621 cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc attcactttt tgacgacgtt
    22681 cctgtttcga ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa atagttcggc
    22741 tttccattag attgaaatgc tgaaagatca taccgatggc ctttctttct tttcgcaatt
    22801 ctttgggaga gagctgactc agattcgtct gattgacaat gacttctcct gctgttggtt
    22861 tctgcagcaa attcatcaca cgaactaacg tacttttccc tgcacctgag taaccgacga
    22921 ttccataaac atcgcctttt tcaatcgtta aatctacaga atctactgcc tgtatctctt
    22981 tttctttttg tttgaaacat acagaaacat ctcgcaattc aatcattaaa attcctcctt
    23041 atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt tcttgaggac gcttttttgc
    23101 gtgttaccac ctcatgttca agctgtgttc acacacaact cctcttcaag tacgatcata
    23161 ctctagcaag atatcggtcg cagccgtcat cgccttacat ctatgctcgg gatgaattgc
    23221 tcgatggcca ttttccaata gtcgttcctt gcttcttctc agctaccgaa gctctctgtc
    23281 aaaggttctt ctatcgtact taccatttca atgcaaataa tattcaaaaa aaaagtcctt
    23341 ttactaacat aaactatgtc agtaaaagga cgacgtatcg cggtaccacc ttttttcgga
    23401 agtttccttc cctcaagccc aacatctcaa aaagagagat tggcgttcgt tttaacggtc
    23461 gaacagttcc gaatttgcct acttatcgac aaattattct ccaaggccat tttcagcttt
    23521 tcttacttga ctcttctcac cagccagagc ctctctgcaa agtttccaaa cttactttcc
    23581 ttttcaacga aatcgatatt ttgttgttgg gattaatcta catgaggaaa actgaaaagt
    23641 caagtacgaa attcaaatta ttttttctat atttattttt ctgttttttt atttaacatt
    23701 tcaataacag ttcgaagatc ttttccttcc gcaagaagtt tcaatttttc cttttcttcg
    23761 acggtataat ttcggacaaa actggaatca ggtatcaaaa caacgcctaa tcctgctcgt
    23821 gaagcagctg ttgctcctac taaagaatct tccactgcaa aggctgaact agcttctaat
    23881 cctgttcgtt ccaaagctgt cagataagga atcggtgaag gcttgttctc cgacacttcg
    23941 tctccgaatg taaacgtgtc caagtaatca gctaatttaa agtgatccaa gatatctgtt
    24001 gctcttttcg taacagtggt cgtagcaatg cctagtttca tatgacgttc ttttaagtct
    24061 gccaatattt cttttgcata tggttttaag tggatggctc cgcttgtcag ctgttgataa
    24121 aaatattctt cacgcttcgc tcggacttcc tgtacttttt cagggctacc tgctgctttc
    24181 actaaaatat caaaactttg tttccagttt tttccgcccc aagcgtcaac gatttctttt
    24241 gggatatgga tatcaaattg ctgaaatccc caaagccagc catcacgata cgttttttct
    24301 gtgtcaacta acaaaccatc taaatcaaat attgcacctt tcatctatac attaatcctt
    24361 tccaaaacaa tacacatagt ttagcacgac tggtccaaaa agaacagaag caaagaacca
    24421 gccttctcgt ttataagagt tcttggaatc gacggatata gattttcttc acagctgtca
    24481 caagcgccag ataagcgatg actgtagcaa ataaccataa ccagtaattg ccaggaagtg
    24541 gcatcaagcc taattcagca ccaaaacttg taaaaggaag gacagaaccg acaataattc
    24601 ctaatgtagt gataacaaac atggcaaatg ttgcgttgct ttggataaat ggtactttcg
    24661 gtgtacgcag tgcatggagc actaaggttt gtgtccatag tgattcgaca aaccatcctg
    24721 catggaataa tgcgatgaaa atcactcgct gtgaactatc caatgtatgg aaacttccgc
    24781 ccactacagc aggacaaata acaaaataca tcaacaagta agtagtaata tcaaatatcg
    24841 aacttgttgg accaaagtaa accatgaatt ccccaatact agatgcattc catttttttg
    24901 gctcgtccaa atattcttta tccatccgat cccatggcaa ggaaatgcag gaaacatcat
    24961 agatcagatt caaaaacaaa agttgtaaag gcagcattgg taaaaatggc aggaacgtac
    25021 ttgcaatcag cacggaaaac atattgccaa aattagaact tgctgtagcc ttgatatatt
    25081 tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat
    25141 ctttttctaa aaggatcacg tctgcagatt ctttcgcaat atccacagct gtatctacag
    25201 aaataccgat atcagccact ttcattgcag gagcgtcatt gataccatct cctaaaaatc
    25261 cgacagtatg accattttta cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca
    25321 attttacgaa tacttcatgg tcttcagcta cttgtgctaa ttcatgatca ctcatttccg
    25381 agattttctc accggtaacc aattcattga tctctagacc tacttgacgg cacactgaac
    25441 gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt tctttcagag
    25501 ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag gaaagcaaga taaccaatca
    25561 aaaccatgtc tgactcatct ttgactgaaa attctccaac gacgcttgga ttggtctttt
    25621 gtgcgacacc aatcactcgt aagccatctt cattaagtga ctgtactttt gcaagtacgg
    25681 cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata aatttagaaa
    25741 tgttcagcat ttcttcaatc gcgccttttg tgatcatttg tgttttacca gctggatcag
    25801 caaccacaac actcatacga cgacgttcaa aatcaaaagg aatttcatcc actttttgat
    25861 aattgatctt atctgtatct aaagtttctg ttgcttcatc aatgatagcc acatctaaca
    25921 aatttttcag ccctgtttga tagtagctat ttagatacgc atggcgcaaa acacgatcat
    25981 cttcatttcc ttcacaatcc atatgatatt caagaatgat tttatcttgt gtcaatgtcc
    26041 ccgttttatc cgtacataac acatcgattg caccaaagtt ctggatggca ttcaaatttt
    26101 tgatcactgt tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga
    26161 tcattggaag catttcaggt gttagaccaa ctgctacaga tagtccaaaa agaaaagctt
    26221 ctagccaatc cccttttgta agcccattga tgacgataac tgctggagcc atcaatgaca
    26281 taaatttgat cagtagcatg gatgttttat ggatgcctaa ttcaaagctt gattgaattg
    26341 gtttttcaga tagcgtttct gctacatgac caaatagtgt ttcatttcca acagaaacaa
    26401 tgattccttc cgcacttcca ctgatgacat tacttcccat aaagacaaga ttttcgtaac
    26461 ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt
    26521 cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc ggacaataaa cgcatatccg
    26581 ctggtatcat atcccctgct gaaagccgaa caagatctcc acagacgatt tcttccatag
    26641 gaatctctgt gtactttcca ttacgtttta cagctgccgt tactttcaca agtgatttca
    26701 gtttttcagc cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga
    26761 taaccatagt gaagataatg gctacaccga aaagatcttt atctccagga gctgctaata
    26821 catattccgt gatgaatgaa ataattccta atgcgatcaa cactaatgta aatggcgtga
    26881 tgtatgcttt caatatttct acaatgaatg gtgttttttt gccatatgaa atcgcatttt
    26941 caccgtaatt ttctcttgaa cgttcgattt gttcttctgt caatccattt gctgatgcat
    27001 ggaatagttc aaataattct tgtttcgttt ttccagcaaa agctgcatag attttttgtg
    27061 tatctttctt tttttccatc tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct
    27121 gcttttggtc tttagctatt catcggatcg atagagaaaa aactgaaagg ggctgagaac
    27181 agatagataa tagccctggc ctttcctcta gatccttgtt tttcgaatga ctgtcatcca
    27241 tttgttctca tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat
    27301 agttattttt ccaattagac cacctccttt ttatttttca ccgatgggga tttgcggcaa
    27361 caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa aaagcatatt aaaaaaagct
    27421 tctcagatct catcgttgag ttttgacact gtacaacgta aaaagcagat acctgctttt
    27481 agctacctta tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga
    27541 cgtttttggg cagtagccta tgtttgtata ggagcctcac ctaacaaatc ttattcggtt
    27601 ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca
    27661 tacttttttt aaaatacaca cgttatattc ctgtctcacg cggttttatt tgtctcattt
    27721 tccacctccc gctataatag aaatatagta ggaaacgagg tgctgttatg gaaagaacac
    27781 ttgttattat caagccagac ggcgtgagac gccatttagt cggaagcatc atccaacgtt
    27841 ttgaagcaaa gggactggcc attgctgaaa tgaagtttga aactatgact cctgaattag
    27901 caaaagaaca ttatcagcac ttgactgagc gcagtttttt tgatgaactg atcgattaca
    27961 tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc
    28021 gtaagatggt tggagcaaca aaagctgccg atgctgttcc aggtacgatc cgaggagact
    28081 acgcgctgcc ggggactgaa aacatcattc acgcttctga ttcccgtgat gctgcggtaa
    28141 aagagatcgc ccgctttttc ccgccattcg atataaacaa accgtctgaa aaagcggcga
    28201 agtgatacaa attctaaact gctggtctag ttgctgggtt cagcaatggc cagcagtttt
    28261 ttctctaagg acctccgttg attctttcct aacatatatt cgagcactgt gaagaattct
    28321 tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa tcaatctttg ataataatct
    28381 gccatcaatg tttcatagat cactgtactt ctttctttat gtgctaagct ccgtttcata
    28441 tcttcattca cactacgtgc ctttttcaac cgaccatttt ctatataaaa cgaagtcagg
    28501 ttcattaaaa aagtaaagag atactgctca tgattgttca actgtgctaa tcgtcgcatt
    28561 ctttttttga ttttcaaata attgttctca atgaaccgat cactgaatat ccaaggaaaa
    28621 tcagctagaa tagatagttc aaattgacca tagtctggta tctgcatcaa gtattcttca
    28681 atgattcggc attcataacg taaaaattca aattctgtct cagatacttc tgttttttcg
    28741 accatgtgaa gattcaaaac caaataatac agatcttctg ttttcttaaa atgcctacgg
    28801 ttattgctga tctctttata aggatcaaag ctgtcttctt gtacccgatc tagcattcgt
    28861 ctttttttct ttaactttat tggttcatcc agctggtttc gcatccattc atattctgca
    28921 agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta caaatgacat ttttttcact
    28981 gaatttgtct caatcccgac tagagaagtc ttaaaacaga ttccttccgc taattgggac
    29041 tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt caccatcata cataatagtt
    29101 ctccttttta atttaaattt ttccacattc aataaataga tacgttaaaa aggaaaaata
    29161 cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac caaccttcaa gcattagaat
    29221 gttgtctaac tactgaaggt tggtttgatt gtttattgat tatttttttg ctgttcaacg
    29281 tctgttcttg ttactggata taagttggtt gttgttttat taccattttt tgaaaagaca
    29341 cttgtcgaat gttcgccttt ttgcttttcg attgcttcat tacgttccgc ttcattttta
    29401 tagtcatatt ttttcggatc aatcgtctca aatccgtttg gcacataaaa ccgcaagaga
    29461 ttttcctgat tcagcttatc tgaaagactt aacgcagtct tgactttttc ctgatctttg
    29521 gagatttcct catcgattcc tgctgctttc gtatcgatga tttctcctgt ctgattattg
    29581 taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa agttcccatt tcgaaaagca
    29641 actacttgat catgctgaga agataataag tctgtaccaa aatgaagata atctttatca
    29701 tcgattccca atagatgtag taatgtcggc aaaacatcga tttctccacc gtattcatga
    29761 ttgactgtcc ctttagtata accaggcata tggatcatca atggcactcg ttgcatctgt
    29821 gcgttgtcaa attcatccca tgtatctgga tctttaccaa gagcgcttgc taaatcttta
    29881 ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca caaagattga attttggtaa
    29941 ataccagatt ttttcaaata cgtaaaaaat tgttccaatg attgatccag ataatgtgcc
    30001 gttcgaacat aattatctac tgtgctattg ccagtattca aagatggaaa gtcgaaattc
    30061 ttgtcatccg tataataagg cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc
    30121 tgttgtagat gctcaagata ttgggcagat tctctgaata aatctttatc taatatccca
    30181 taacctaacg tttcgtcgga ttcatcaaaa taagatcgat caaagaaatt gtcataacct
    30241 aagtttttat atacatgatc tctattccaa aaactagcta cattcccatg gaaaacagcg
    30301 cttgtatagc cttgcttttg tccaaggatc gctggtgcgg cttgaaagac attgtctgac
    30361 ccaagttctg tgaataatga gccttgaggt aaaccaaatg tgccagtttc caacatattt
    30421 tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa gctgatcgtt
    30481 gcttgattct ggaaaatact atttaaaaac ggtgtgactt cttgtccgtc taccttcata
    30541 ttaatcaaaa actgctggaa actttctaaa tggagaacga tcacattttt ccctttagcg
    30601 atcccaaatg tttcgggatt tggctctgca taatgtttct ttgtataatc taaaacttca
    30661 tcaatcccat tactgcttgc atgcgcgcga acactgctag tcattcctgt tttgattccg
    30721 tcataaatag tatacgcatc gattcctaga tatttgacaa tatatgaacg gtcaaatgtt
    30781 cgctgcagca attgtggacg gttggcttcg ctcagcgcta gatttcctga aaaaataagc
    30841 cctgaaagac aggtaacagc gatagccatc ggtttgccga cagggttgct tttgataggt
    30901 attttcttga ccttcaacca aaccagtagc ccaatgaaca cagcgatatc cagccagtag
    30961 atgacatcat ggggcttcat caaagaaaag gagcttcctg acaatccttg cgacactttc
    31021 gaaaagccta atacagattt gactgtaatg aaatctgtga attcccgata aaaaataata
    31081 ttcaagtaca acaaaagcgt atttaaaata tcaatgatca aaagaatagc taacgctggc
    31141 tttggctttt taatatacaa agaaaggcca aaaaacaata aggtagtcgc aattgggttg
    31201 atccacaaga taaagtactg gatcgtacct tccacaccta atgaaaaatc tgcatagtag
    31261 gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca aaacgattgc taaacgattt
    31321 tggcacaatt ttttaagttg tgtcacgtat atcacttctc tctattcaca ttttttctta
    31381 ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt atcttaagat ttttttattc
    31441 tttctaaaga ttattccgtc cacttttcac agcataaaac cgctgcgatg taagcgatta
    31501 aactaatgaa gagataaaag tcttgtggtg accgcttcac tccacggtat tcaaaagcca
    31561 gtttctcggt attcgtacaa aaaaagaggc tgtgacaaaa ttcttgtcgc agctccttat
    31621 ttcttgatgc aggacagctt tttcacaact tcttccttaa acggtgttct cctgccaact
    31681 cctcggccac aagtcacaat atttataaaa catgagaagc tgttttttga aattgttcct
    31741 tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt caatcagctg
    31801 accctcggta ttagattatg tcaggacgct aattttacaa cctcttggta aacggtgttc
    31861 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatataagga gctatttttt
    31921 gaaattgctc cttgtggctc agagttaaac ggcaggctca agacctcttg atacacggtg
    31981 ttcaatcagc tgaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg
    32041 ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctg tttttcattg
    32101 tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg cttgattttc
    32161 ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc ctgtcgactc
    32221 gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt tccattccgc
    32281 taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga tcacttttcc
    32341 atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg tcaaatgctt
    32401 gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga tattaataaa
    32461 ttgattttct tcttttcctt ttggcaaata aatgtccaat tcgccttttt cgtaattggc
    32521 agttatttta tactccgccc accaataagt tgagaattct tttttgaaat tatctactga
    32581 ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg tcatttttcc
    32641 tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa
    32701 cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat tctattatat
    32761 acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt
    32821 tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat tctttcttaa
    32881 gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat gacccgattt tttgattgtt
    32941 ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt atatgcattg
    33001 gtccacgacc agttatccat acatgtccac atatgatagc ctaaacaatt ggcaccttct
    33061 tgaatggctt gatggacata tttcagatga gattggacaa attcgatccg ataatcatcg
    33121 tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat accgttttca
    33181 gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat atcatagatt
    33241 ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg catttcataa
    33301 ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt ttcttttgct
    33361 tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt ttctttgata
    33421 accgtcaaat cctctgcatg gatcacaggc atgagatcaa gttcttttac tatctcaatc
    33481 aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt gaagaacgca
    33541 tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg atagcttggt
    33601 gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta agatttgatg
    33661 gctaacgctg aagccaacgc ttcatgatag ccaacttgga tcgctttttt catatctgac
    33721 tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat tggttcatta
    33781 tgagtaaacc atttttttac tttcgaacca aacaattcaa aacaactttt ggcatatttt
    33841 tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag atacatcggc
    33901 atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa
    33961 taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc aggtatcaaa
    34021 cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat caactgtaca
    34081 tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg atcaaagaac
    34141 ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc atcgcctgaa
    34201 atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt tgctggaaat
    34261 tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt tgattccatg
    34321 tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt tgattcgttt
    34381 taacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga tcgtgatttt
    34441 cggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg caatatcctt
    34501 cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca tattgtaatt
    34561 gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt aatagccgct
    34621 ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat tttggaccgc
    34681 ttctttggct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg cagcaataaa
    34741 tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta cactttctcg
    34801 atcattcact aatattgtat caatacctgc tggtccttct ggacaattca atgcgataac
    34861 tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg ataagaagac
    34921 aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa ttttttctgg
    34981 ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta ctctttcgat
    35041 ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa gtccaataga
    35101 gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt ccaacgctga
    35161 aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat tcaagtaacg
    35221 ggaaactgtt cccactgaaa caccagccat cttagccaca tcacggatcg atgccataca
    35281 ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta tgatgaccgc
    35341 attctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt cagtagagca
    35401 acttcctctc actattaatt atacttaaac aagtatttga accggttcat ttttaatata
    35461 gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg gcggattttt
    35521 tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg tgcgtttttc
    35581 tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc tatcaaatgg
    35641 gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac ttttgacatt
    35701 gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa ccgattcttc
    35761 tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa aagtacgtaa
    35821 agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg aatggacacg
    35881 cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac
    35941 agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag aaaataaaaa
    36001 agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg aggatggcaa
    36061 catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct tcttttctgc
    36121 tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat agtcctgatt
    36181 attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa ggaagcttga
    36241 gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt gaatcttatc
    36301 ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa actcactatg
    36361 gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca tcagtaaaac
    36421 aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat ttcaaatatt
    36481 tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt aatttttctt
    36541 tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg ctggttatcc
    36601 gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg gatctgacgg
    36661 atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc atccacttgt
    36721 attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt caacgtttca
    36781 taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc acgttttttg
    36841 atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc aggccgtttt
    36901 tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc gtactgctgt
    36961 acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag gctatcttca
    37021 tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat tgctaaaaat
    37081 cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt tgaataaaag
    37141 agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc aatctctttt
    37201 gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat caaaatcact
    37261 gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac aggcaatgtt
    37321 tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag actttgtgta
    37381 atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt tttcaacata
    37441 gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc aaagtcaagg
    37501 atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta tacttcctgc
    37561 gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg agagtagaaa
    37621 cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg atcgatttgc
    37681 tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc tccttcaata
    37741 ataactgatt tttaatagat actgctcgta attcattgac cagttccaat ggattgttgg
    37801 aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca aaagtttcta
    37861 ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt ggcttgtcgg
    37921 gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat gtacctagac
    37981 ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt ctttcatcga
    38041 tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt tcaaaaagcc
    38101 cattcggata gatacctgtg acttttccgt tattttccag tactgtttta ctaatcactc
    38161 ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt
    38221 ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg gaaccgcaaa
    38281 aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc attctacacg
    38341 tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata ggatagacgc
    38401 cactatatat agtatttaga aaaaaatcat ttttcagcca ttttatagat aaaacaaaaa
    38461 taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact gggatttcgt
    38521 cctatatgcc aataacgtgt attattttaa acaacaaata cttgtttctt ttattaacga
    38581 aaaaatcatt ttgttctcgt gttatgtttc actgataaat gatgtttctc taaataaaaa
    38641 agccgacgaa ctaaacgatc aaaatttgat catttttgtt catcagcttc tcctaaaaat
    38701 ctttttgtta acgataaata gctggatttg taaaaatatc acttgaatcg tcactgctgg
    38761 aagaaaattc agtgataacc gcacctgttg cgcctgcctg gaactagtgt ttgatattgg
    38821 gctcgaacgt gtactgatct ctagcatgaa gaataatttc tttttttact gtataataac
    38881 tttctttcct ttttggaata gtacaggaaa gtttttttgt ttcctttcct tcgacataag
    38941 gtataccaga ccctttcgac aacgaaaggt tctcattttt ccttcatcgt catttcaagg
    39001 agggtgccga tgctctggac agtttcgatc gctcctaaac caaaatctgt atattcgatt
    39061 ctttctaact ctgattcttt cataccattc ctgtctcaag gtacatctat tgtacttttt
    39121 tcttgtaatc cattctgcct ctcctaacta attgtcaaat catcccaaga tatgtcttct
    39181 tcctcttttt cttcttggat gatttctgca ttcggatcta ctggtttttt caataataaa
    39241 atggttattc ctgtgacaaa agaaccagct aacaatgcac caatgaatgc ccatggtctg
    39301 gtcatcgtag cgactgcaaa cattcccgat gccggaatac ggctatctgc gccccaaaac
    39361 atgcttaatc cgccacctac tgtcccaccg actcccgttg ctatgacatt gcgtgccaag
    39421 tcgttcaata cgatagggaa acatccctct gtgatctgac agatccccat aggaaaagcg
    39481 gtcttcaatg tatcgatttc ttgacgattg agtatatttt tacgaatgat tttccctaag
    39541 aaataagcga tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa tacagtgatt
    39601 ggttcataga ttccttctga aaatcgtgcc acaccccctt atccagaata aatggtaatc
    39661 cagaaacagc cgtgtatctg cggtaataag cctaaccaaa cgaaaaatat gagaagctat
    39721 ttttgtttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttttttta
    39781 tttatgtttc ataatcaata tggtaaggat gcagctccgt atggtagacc gcctccgaat
    39841 attcaatcat ttccccaatt tgcgtagacg atttccgtat ccgtttgatg ccgatctgtg
    39901 cgtctgtttt cagaattcct tgttcttctt ctgttaaaaa tactgccgag aaatcatcag
    39961 aaaatgagtc aatagtatat ccttttttcg tcagcaaacg atacaaagat tctttgccaa
    40021 attcttctct gctgaattgc atcatttctt gtggaagata atgaacaaaa taaatataag
    40081 gctgattgtt caaataatat aagcgcttaa aacaaacagc ttgctcaccg aaaaaggaaa
    40141 acaaaggatc ttccttcttt atatggacca attgcaaatc cagcgtttct ttccggactt
    40201 catatcggga gttctccaag atttgcgtaa agcttgccgc tttggataat cgattgtaag
    40261 gacgattgct tagaaccgtc gtcccacgac cacttttctt tgcgacatat tcatctgatg
    40321 ccagcagttc gattgccttt cggacagtga ttttgctgac ttgaaacagt tcttctaatt
    40381 cactttctgt cggcaacatc gaaccgactg gatattttcc tgagaaaata tcttctttaa
    40441 ttttatctgc gacttccaag tatagtacac gtgtcctttt cataacgtca ccctttgctt
    40501 tactattact aaaagaatag cacataaaaa ataaaaaaag aacacaaatc acatgtgaga
    40561 agtgtgttct ttttcattca gctgctttaa ttacaagttt acgccatttg aggcaattac
    40621 ttctttatac caatcaaaag attttttctt tgatcgattc agtgtcccat ttccttcatc
    40681 gtctagatcc acatagataa agccgtaacg tttactcatt tctcctgttg atgcactgac
    40741 aagatcgata catccccaag gcgtatagcc catcaaatct acaccgtctg ctactgcatt
    40801 agccatcgct tcgatatgac gacgtaaata atcgatccga taatcatcaa taatcgtgcc
    40861 atcctcttcc atatgatcaa tggctccaag accgttttca acaatgaata atggtttctg
    40921 gtaacgattg tataattcat tcaatgcgat tctcagccct tctggatcga tctgccagcc
    40981 ccattcactt gcctcaagaa acggattttt tactcctccc agcaagttcc cttctgaagt
    41041 tgctgcttgt ggatcagtct cattgattgc cgtagacata taataactaa atccgatgta
    41101 gtccactgga tattctttta gcaactggaa gtctttttcc gtttgctcta atttttccgg
    41161 atcgactcca tattttttat gggtacgcgc tgtgtatgca gggtattctc cacgtacttg
    41221 gacatccgtg cagaaaaaat tgaattcttg gttttggatc attgttgctg cctgattgac
    41281 tggattggca tcgatgctgt aagttgttgc atagataatc atgcagccga tttggatatc
    41341 cgaacgcaat tcgtggccga tttttacggc tttgctactt gcaacgaatt gattatgcca
    41401 tgcttggaaa atattttggt actctcctgc tccattgctt tttactaatc cttgacttaa
    41461 agctggaaag tgaaaggccg aattgatttc attgaaagtc atccaatatt tgactttact
    41521 gctgtatctc tctaatactg tctgagcaaa tcgttcatag aagtcgatca gttttcgatt
    41581 tttccagcca ccgtattctt ttgccaaatg aagaggcatt tcgtaatgag aaatcgtgat
    41641 cacaggttca atatcatatt ttaagcactc atcaatcagc tgatcataaa attctaatcc
    41701 cgcttcgtta ggagtacctt cgtctccatt tggaaaaatt cgtgtccagg caatcgaaaa
    41761 acgataacat ttgaatccca tttccgcaaa caaggcaaga tcttctttga aacgatcgta
    41821 atgatcgatt cctcgatgat tcgggtaaat atatttttct tgatcaatcg tccagtcaaa
    41881 ttcttcactg ccgataatcg caaagcgctg cttacctcca ggcatagcat ctgcaacgga
    41941 tagtcctttt ccgtctcgat cataagcccc ttctaattga tttggcagca gttgctccac
    42001 cccataaaaa tttttctgaa aaacgtgatt gcatcctgat ttcctcctta ttttacaatt
    42061 gccagaacat cttcgtttgc aataatctct ttttggtcta attcaagtat atcggcaaat
    42121 gcatcagtat tcgtaatgac aactggagtg attacgctat agccagctgc tctaattgct
    42181 tcgatgtcaa aacgaatcaa taaatctcct gctttgacga gatccccttg tttgactaag
    42241 atctcaaagc cattcccatt taattctact gtatccatcc cgatatgaat caaaatctcc
    42301 gctccgtcag tcgttgtgat ccctacagca tgtccagttg gaaaaatagt cgtgatctct
    42361 ccgtctgcag gtgcaactag ttctcctact tctggatcaa tagcgatacc ttttcccatc
    42421 acgccagaag aaaaaacttg gtctggtact tcactcaatg gcagtacttt acccgttaaa
    42481 ggacttttta aggtatgccg tgctgaagta atcggttttc ctgcattcgc atgtttgttt
    42541 tctaattgat tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc tacgaatgca
    42601 agaacaaaag caattcctgt agctattact gctactgtca catttgactc tacaccatcc
    42661 accgtattaa tgaaagtcgg aatcgtcaat aagctgacca aactgctgga gaaagctttt
    42721 actccggaaa atccgataat agctccacca atacctccag aaatacaagc ggcaatgaat
    42781 ggtttcttca aaggtaatgt cacaccatac acagttggtt cagtgattcc aaataacgaa
    42841 gtcactgtag aagaaatccc taatgctcga agtttcgtat cttttgttcg caatgctaca
    42901 gctaaagcag ccccaccttg tgccaagatg gctggaagaa gcattggcat caatacatca
    42961 aacccatatt gttcgatatt caaaaacatg atcggtacga atccccaatg catgccgaac
    43021 atcacgaata cttgccataa acttcccatg attagacctg ctacgattgg actaaatcca
    43081 taaatcgagt tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat tggtcctaaa
    43141 gcaagaaatg ttaatggaac catcactact aaaacgacaa gcggaactaa aatcatttgc
    43201 agaaattgcg gaatcacttt ttttacgaat ggctctaatt tactttgaac ccatacggct
    43261 agaatgattg gtatgacaga agaagtataa ccagatggtg aaagaatcac tgggatccca
    43321 aagaaactga tagcttctcc cgcaccagca actgctgtga tagctggata cacaagtgcc
    43381 atagcaattg ctactgctaa aaactggttg gctttgaact ttttagctgc tgtaaaagca
    43441 agcatgacag gtaagaaggt aaagacacca tctgctgcag caaacaatat ttgataagta
    43501 ccgctatcag ctgtcagcca gcccatcacc gttgccaatg ataagaatcc tttcaatacc
    43561 ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac tagagataat atcgatcaga
    43621 cgattcaaaa tatttttgga ctcttctttt ccatcctccg aaactgcaag accttcctca
    43681 tccacgatcg attgataaac atctgctaca ttactgccaa tcactacttg atactgaccg
    43741 ccactttgta cgacttggat cacgtccggg tcttctttta acttttgcgt atctgctaaa
    43801 gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact tttgacattc
    43861 ttttgtcctc ctaccgcttc ccatacacga cgaccaactg cttgattatc cattgttttt
    43921 tccttctttc ttttttattt aaaaaataaa aacccagatt gaaaaaaaca gtcacaaaca
    43981 ttcgtgaacc tattttttca atccaggttt tgcctgccat acagtaacaa tccttatgat
    44041 tggatgcttt ttttctcact tactactctt tgaatatgaa tcgttaaata aatctgctct
    44101 gcgatcccca tttcctgttt ttgcgtttgt cgaagatact cattgatccg ttcgacacaa
    44161 tgaaacgctt ttggatactt tccttgaact aatgcgtaga gaaattcatc ttgctcgtca
    44221 gatccttccc ctttgagata acgctgggca aaaaactgca gatgagtaat gatacgctga
    44281 taattcaaag attcttcatc gaacacgatg ccgaagaatt tactgatgat actcagtata
    44341 tctcttacca ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc ttgttggcta
    44401 ttcacaaaat gaaaggcaat aaaaccggct tcatcctcag aaagttccac acccgtcttt
    44461 tgtttcacga acaaaagagc ctctttcgct gcttgatatt ctttcggata aaattttttt
    44521 gtttcaaata ataatggatt aggcagttgg atattttctt ttgctcgtgt gatcgcataa
    44581 tgaagatgat cggtcaatgt cagataaata ttaggcgaaa attcaacttt tagtttatct
    44641 tcagccagtt cgactacttg tttgacccat tcgatctcct ctgccggaat atcagccatc
    44701 agctgctgga attggttttt agcaaaagaa tctcttaaaa caaattcttt ttggaccgct
    44761 tctggcgaaa tctcgtcccc aatctttttt tgaaaagcta gtccttttcc catacaaacg
    44821 acttcttccc cgaatccatt tttcgaaatg acgacattgt tattcaaaat tttatcaatt
    44881 ttcattttgc ttcgccctct caagaaaaat aaaaaaacct agaattcgcc tactgtttct
    44941 tagctgaatt ctggttttgc ctgccgtaca gtaacaatcc atttcttatt caaaatcatc
    45001 ataccatcga atttttattt atgcaagcct tttcatataa aaaataaaaa aactggaaca
    45061 caagtctatc tagaaaacaa ccaaatcaga gatgcacctc tttgcgtttc tctttttttc
    45121 agcaaattgg tttttcctta tcgattcata tcctttggct gtttttaatc tgtaacgact
    45181 tatgtcccag tatcctaaaa tgtttttgtc tctagattga aagcagatct catatcatct
    45241 gtcgcatctt ttatcgatgt aacactttcc agttctcctt gtacaactag tcgcgtgacg
    45301 gcgtccatgt cgccacaagt aattagtgtg acaatttctt ttccctcgac ttcattcagt
    45361 aattgggtat ctgtaggagc aactttttct ttaaaaaatg ttttgtatgt atacacattt
    45421 tctaaatctg tcaaatagat cttggcaccc atctctaaat tttctagcgg tgtaaacagc
    45481 aattcagggt tcgttgcacg atggcttgct aaggcataat ttccttttcc catttcttga
    45541 tctggagata atgttcctgc tccgtaaagt agtgacgtat tatccaagcc tttgaaaacc
    45601 ggtaaattga ttgaaactga agggactgcg atactagcaa tcactggtag atctgttcct
    45661 ctcatttgcg acctcatgac cccttctgtc gtcattggtt ctaccgcatc aaagtcaaaa
    45721 ctgacatctt tatcgttatt ctttttcaga tcttcttttg ttacattcgc tatagcatat
    45781 ttatctcctg tctctctgac aaaataatcc ttgatctgtt cattgaaaat caaagctagt
    45841 cctactaata acaagaggaa caagaggata ttgactagcc atctttttgc ttttcctttt
    45901 tttttcattt tctctccctc cagactcatt ttactgtttt tcttttgctc tttccaacag
    45961 ccgaatgaca catttctaca actctgtgta tttttttcgt acacctgcgc ttttttctac
    46021 aggatatttc aaattatttt tgatcaattt ttcttcaatg atctcatcta gatccaggtg
    46081 aaggttgtct gccatcatgt atgaatagat caatacatct gccagttctt ctttgattcg
    46141 ttctaattgt gtttctttta cttcttccgg cgttttccat tgaaaaagtt ctaacaattc
    46201 agatgcttcc aaagaaatag agatagccaa atctttttcg ttatgaaatt gtcgccaatt
    46261 acgttcatct ctaaactggt tgactttttc catactattc atttgcttgc cttcttccaa
    46321 tcacttctta cttgattaat cataccattt ttttacataa aaaatagatg atgcttttaa
    46381 aaaaactcct gttttctttt atttaaaata aatgtcatag aaaatcaata atttaggtag
    46441 ccaccatgtg tatgcagtag cgtgtcttct tcccttgagg ctttttgttt ttcgatacgt
    46501 tcttcttccc aaacagacaa cgctactaga atctctctta atgattttcc ttcttcggtg
    46561 agcgagtact ctactttagg cgggacttgg ttataggctt ttcgatggat actttcatct
    46621 tgttctaact ctcttaactg ctgggtcagc attttttttg tgatcgtagg gatttgcttg
    46681 cttctccacg cctaaaataa gcgatattgc tcttcccttt atctggtgta agcaaagcag
    46741 ttagcatttt gacgatcgtg ggttccgctt ttcacttctt atctacttag tatcatgaag
    46801 tatactttcg tactaatact aagtagcgga agtgtcaact actttgttta actacatagc
    46861 aaaagaaaca gaagggtgac aacggtcatc acgcttctgt ttcttttgaa tatgctgtgt
    46921 tgttgtctct atctccgtgt agactttcct caaacttatc ctttgacagt aagtcagtag
    46981 gctgatttgc tctttctaga tgccagatac atttctacaa aaaaatagtg attctgtttt
    47041 gaaaattcga tttttagttc tgcattttcg taaacatcca acattttttt cgcactgtat
    47101 agacctaacc cgccattttt tttagaggtt tttcctcttt tgaccatctc cgatagcgtc
    47161 aaattcgttg gatttgcctt gttcgctata ctaaaacgtg atccctcttt ttcttccagc
    47221 agattgatcg taatcggact ctgatcggct acacgatgat cgaatgcgtt agaaataaaa
    47281 atagataaac agcgaataaa aacaaccaga tcaatcggga ttgttttcac ttcgacagga
    47341 ataactactt ggatcgggat tttctccgat tctgcttttt ctagaaattt taaaaagaca
    47401 cccctgacag ctggggatac aatatttgtt agctgttgta tttgcggttt ttcaaaaata
    47461 gctgcagaat gatcttctaa tatttctaaa aattccaacg tttctttttt attttcctga
    47521 agagtaattt ttaatgaaaa cagtagactt ttataatcat gtttgaattc ctctagatga
    47581 tggtaatatt cttcatttat ttgatacagt tgaagcgatt cctgatgttg tctttctttt
    47641 acttggaaac tgatcaacat aatggaaaaa aacaaagaaa aaaaactgat gactatcgac
    47701 aaaagagata gaagcagata attagatgag cggaagtgaa cacttcccaa taccaagtaa
    47761 atcacgcccc agcttaataa aatacaaata cctccaaaat agtgttcagt tggataagta
    47821 aaaagcaagt tgagaaatct cgtctgtttg tctatacttt ttactaatag atagaaaata
    47881 aatgcgataa gtagtgcaaa aaaacttgct gttccaagat gtctctttcc taaataatcc
    47941 atcagatcaa aaaccagaaa aaagctgagt ccagtgatag caaaaaaaag attactgatc
    48001 aaacagctga taaacaggtt tttcgtagta aaataaaaaa gaaacatcaa taataagcca
    48061 actaggtaag gaaagctcac tttagggata ggaagtaaaa gtgtcaccac catcaaagct
    48121 atacaaccaa agctgttttt tactagcagt tgtttcgaaa agaataaata gatacctaaa
    48181 tctaaaatca aagtaaaggt caaataaagt gtcactgttg cgaccatctt attcctccat
    48241 tgttttgttt caaaatgtac acatattttt ttaataggag cctgcatatg aaaaagtatt
    48301 ttatcataga agataaccag tttcacagcg aatggatcat cagagaattg gtcaatcatc
    48361 atatcgagaa tatcgtgcag ctcacttcta tctctcaaat caatcagttg atccagttgg
    48421 gcactattga taatcacgat gttttcttta tcgatatcga cttgaagcaa tcactcacag
    48481 gtattgatgt cgccgaacaa atacgtaaaa aaaatgcaca tagtaatatt gtttttttca
    48541 ccgcttatac gaattttgca gtagagatca tcaatcggaa aatccttccg attggatata
    48601 tcagaaaaga caggaattta tctgaacaaa ttcgtcaagt gctagaacag atcaattgga
    48661 aagaacagca gatcgtaaat gtcgataaaa tctttattga tcaaaaaggt ggaagtctcg
    48721 tcctgtatcc tcgggaaatt ttatatattg aaagtatcaa aggtttgaaa aaccaagtta
    48781 tggtcaaaac aactacagaa gataaattag tgaatacttc tttaaaacaa ttgaagacag
    48841 agttagaaaa gtatgcttac tttttacttt taaaatctta ctgtatcaac ttatcgcaat
    48901 tgcagaaaat tgattctgct cattatgtgt tagaattttt caacggtgac tccttactcg
    48961 ttggtcgaaa gatctttgaa aagacaaagg aacgtttcca tgacttccaa aaaactgctt
    49021 cgagttgatg aataagagct gagacgtaag catagcagat ttcagggagt gggccttgaa
    49081 tcggctccac tccctgaaat cgttgatatg attttacgct tcctcttctg taggagcatc
    49141 tgttaacgac catgtgaatg ttgaaacgta gttgtctaca gcataattgg atacaggaac
    49201 aaataattcc actgattgtc tcccttgttc cacattctct cctaatgaat aaatccatgt
    49261 gccaatccct tcatttgctc ctgcagttac tagatctact gcctgatctg ctgggatttc
    49321 gattccttca gcgccagttc cagaaacggt agatggcatt ccactgctat tagttgcgct
    49381 atttcgaatc acggcgtttt tgaatttcac ctttgaacca gttagtttat tcgtatcaga
    49441 tgttgtacca ataaattctg ttcgtttgac tgataatttc caaccggcat aatttccgct
    49501 tttatccgtt acttgtacga aattctgtac ttgtgtttgt gctccagtac tgtccgttcc
    49561 gctttgtaat gcagcatata ctgtttttgc tgtacctaac ttgattgttc caaaatcaag
    49621 atctggagct tggtcaatcg tcagtaatcc ggatgttccc tgatttggtt cgtcaggtgt
    49681 aaaaggagta tcaggatcat ttggatcaac aggaggaact ggaacaacgt cttcaggatt
    49741 tgcagcatct ggtttggtca cagaaatcgt tccttctgta gtggctgagc tcttattctc
    49801 agcaaaaaca ctttgtgtac ataataatag acttccagct agaacggtac ttaatgcaat
    49861 tgtttttttc atttcttttt ccttctttct tttaaaataa ataatccagc aaataagcca
    49921 ataagaatga ctccaacaag tatcagccat cgccagtcaa atgtttctgg ctgatttttt
    49981 aaatcaatat ccttttcatt caattggtcc gctgcatcat cagttatttc aaaatcggta
    50041 tggagattcc agcgtttttc tttccaagca cccttcacaa ccgcttgata atgtcccgga
    50101 gctaattttt ttccttctaa tgggacaagt aaatcaaaat ttgtattggg tgctacatct
    50161 attttttctt tttttattga aaggtaaggc tcttttgttt cttttttgta gatttcaacc
    50221 gatgtagtca agccgaacat ataagaagca tttggatttt gaagattcgc cttaatcaca
    50281 tttcttccat cgctttgatc cgcataagct ttttttaatt tcaaatctgg ggaagactct
    50341 ttttcgctat ttcaaatcaa gattgctttc acttgagaaa actgattgac gacaccaact
    50401 gtacttttgt tagaaacgtt ctcttttttt tcttgaaacg tgatccctcc tgcgaccaca
    50461 ccagccagct ctttttctgg cgctttcagt gaaagtgtct cagtgaccgc actttgagga
    50521 ggaacagtta tttctcccac tgtttttgct aagtttttca tatcataact taatgtttca
    50581 tcaaatttac tttttgattc tccatattcg atcacaccgt ttgaattcgt cgtagcggag
    50641 tggatcaatg tctcaactgt tactgattta tctgttcggt taaagaccgt gtaggggatt
    50701 tcctgtgttt tcccaggctc catccttaca tttatatatg ttttgttctt atcaatctga
    50761 ttagccggta attcaaaatc taccgcaaaa ttgaattcgg aggcatggac aggtgtctgc
    50821 caaaggatga atccaaggag aaggatcata agtaaagagc agttgtttcg atgactttta
    50881 ttccatattc gattcatcaa tcatcctcct tgatctttat tctgtcggcg catctgctaa
    50941 tgtccatgtt atctctgctg agtaattacc agctatctga ttttctttgg ttaagacaag
    51001 tttaaacgcc cgaccgttat ttaaatattc tgaataatct gcttgcacat tcgttgaatt
    51061 ttgtcctctg acaatttcca gttcttcttc attcactaat tgatactcct ctcctttctt
    51121 gaaatatagg acatcctgca tagaaaatcc tgcatcattt gtgaaaggct gagataattt
    51181 tatcgttaag atccacggat ttttcgtttc tcgctgatca taaatccata atttttcctg
    51241 tatatccatt tggccaaatg cttcttgtgt caaaccagtt gctttgatct gaccaaaatc
    51301 gagtttggaa ggggcaagaa atgagagacc accttctact acgatcttcg tagcaggtgc
    51361 aaaaggcggg gcatcatgat aaacaactgg cgtatcactt ggattgtgat tcccaatttc
    51421 atcgttggta cttggcttgt tctgcacacc cttagcagta gcttctcctg cttggtcgtt
    51481 taatacaatt tctaatacat ctcctgcaaa aagttgattt tctgggatag aaagaacaaa
    51541 ttcccctgta tcatcaatag ttgtttctga caaaacagct tgattttttt tcaatgaaac
    51601 caaggctcct gcttcacctg tacctgtgat cgtgcgagaa gctgcattga tcaatgtggt
    51661 acttaaatta gcaggttcag gaggaatcac atcgactgtc gttttggcag agtccagttc
    51721 tgtcacttct tggccacctc ttactttttt agcagcaaca acttgcactt gatcctccgt
    51781 ttttaatctc tcatcattgg gtacttgaac ttcgaacaag ccgccttgtt cgccttctcc
    51841 ccatgggtta tatcctgccc cttcattttc atctgtttgt ccttgcgtga gagcagtaat
    51901 ttctacagat gatttcgtcg tatctgcgta agtaatacgt aaagtcactt ctacttcgcc
    51961 tgtccaagca tctcttggct ttgcctcgac accttcggga acaacgacat gcccatagat
    52021 ttttttatct gcattcgttg gcactcttaa ggattcaatc atcgggagct gattattggc
    52081 actgattcga ttataatttt tcattccggc acctgtctga gcaaattcat ttgttaaacc
    52141 tgcactaggg cttgatacga aattgcctaa gtcggtccca ttgatcgata agtctgtcat
    52201 atcagaaaaa taatccgggt ctttttcaaa gttatcgaat gttgcgcctg tttttcgcca
    52261 taaagcgatt tctgaatggg cgatctttaa agaagaatct ccatttcctt ggaataccca
    52321 gccgcctatg ccattggtct cgcttccaat ccgtttgttt tggaaatcaa aataggtagg
    52381 agattctaag tccacattca acacatcccc actgatgatg ccggtcttat cttcccctgt
    52441 tttcccgacc atgactaaat aagttttttc acctgccgta aaatttaaat caatatctcc
    52501 agttgtatct acagttgctc cggaatctgc gcgtatatcg atatttgatt tttctccagt
    52561 gactgtaaaa tcagctgaac ctggactttc tagaaaacca gaagcgtcat aaaattggat
    52621 cgcttgattt ccttgattga gtctgttcac gtaagatttg ccatcacctg tatttcttac
    52681 cctcaatgag cctccattgg ttactgtcag cttttgcgta ccattctcaa aacgaactgc
    52741 gctggaacgc ccactctgct tatcgatgtc gataactgat ccggctcctt ctacgatcaa
    52801 agaaaggcct tttcctacaa aacgcatccc attgttcccg ggttgatcgt catctccagc
    52861 cattttgacc gagatttgtc caccacttga tacatggatt tgactagaat ctccttgcaa
    52921 gcgaaatccg cccgtagttg ctgtattgat ggtgattttc ccaccattcg atacagtgac
    52981 tttcgatcct gaacctctca tttggatgat tccgcgattg ttgtcattgg taatattgat
    53041 ctttgtattt accgaaaatt cgcttcccga accactgact tcaaacatga cattaccagg
    53101 tagcgtatcg actgcctctc cttcatcgct aacgccatct gcccgatcat aatcaagaga
    53161 gacctttgaa ccgcttaaga tatggattcc tggcgctgtt tttccactac cagtctcatc
    53221 tcctgaatct agaaaaccgt agaatttccc tagatacggt ccataaagtt gactaccatt
    53281 gatcacattc aattgatagc ttctacaaaa ataggcttta cccggagcgg cattcagtcc
    53341 ttccttcact ggttggacat ccaacacacc ggaaacacca tctaatgtga cagttgcacg
    53401 aggagcgtaa atcagtccta atttggaccc atcatacaaa tcaagtgtcc cggtaaattt
    53461 tatttctccc gtaaaatgat atctgcttcc ccaaaaatcg gtcatataag caatgataat
    53521 ggcatttcca tcgatatttg gtgtcaatcc acctggatgt gcagctgttc ctttaaaatt
    53581 ctggaactcg atcgactgag gtatctcatt gatataaata tggtgtgtgc cgaaattgtg
    53641 agcgaatcca ccaaaatcaa taacgatatc tcgtttgatc tctaattccc tgtctaaagt
    53701 caaaggctga atcagtcgga tcttagttac tgattcgtct gcgagaagct gcgccagttc
    53761 ttctgttgtg gcagcttcgc tgtacccatc cgctgaaaca gctcttgcca gtgccgcttc
    53821 actgctatca gacgtttgtt cagctacttc tgaggtctca cttgtcttat tttcttcttg
    53881 ccttttatta gtagaagcct cactttgaga ttctgtagtt tcagaagtaa tcgattcttc
    53941 tgaaaaagta gtcttgtcaa aactttgatt ctctgattgc gttgattcgt ttgttttctc
    54001 tacatcggca aaaacggatg tcaccatcaa gagagggaag acaaacaaaa tcgacgcaat
    54061 cagctttttt acacattgat tcattctcca ccatcctctc tgacgttttg acaattttat
    54121 tttatcgttt taatctattt ccaattgtac tttcatattt aaaatatgta tttttatgga
    54181 aaaatttttc tattaggtgt aaatttcttt tttgtacaaa aaaaaggctg tgacaaaagt
    54241 cttgtcacag tcccttatcc cgaacaagcg gtgttctccc gccaactcct cggtcacaac
    54301 ctccatttta acaataaacc tgtaaatgtg agggcaatac tttgattgtc acaggtaatt
    54361 tatccccttc atctccatca acattcgtcg tcaattcttc ctcatcagct aatgacacaa
    54421 ctaattgctc acaagtaagg taggctagat ttttcgtaga ttcatctact ccttttaata
    54481 ggtctggcaa tgttttaagc gtatcgatca aagaagaatc ttttaaataa accaaatgga
    54541 gcttaccatc atccactttt gcatttggaa gtaatgtttc aaaaccgccg actgagttag
    54601 ttagtcctat cagcaaggta ctgctctcaa tttcttcctt ctttccatca acttctacgt
    54661 gaaacgagta ggattgtgtg gaagctaact gtttgattcc tgacatgaaa taagctagtt
    54721 ttccaaattt cgtcttcttc tccgcatcta catcattgat tgattcaggg atagagccaa
    54781 ttgcgacgac attcataaaa tagtcgtcat ttatttttcc aatatccaat ggcttgactg
    54841 attcaatcga aaaatgattg atcgcttcct caggttctag cggtatccct aatgctcgag
    54901 ccaagtcatt caccgtaccc aatgggaaaa atccgaaatt tggccgatgt tcttgttcag
    54961 cgattccact gattccttcg ttgactgttc catctccgcc catgacaaac acactatgat
    55021 acccttccgt agccgcttcg cgagtaaagt tttttgcatc ccctgctttt tttgtatgca
    55081 agacgaccac ttcatcaaac accgattcca attttgctat cgctaattgc tcaaattcct
    55141 ttgcttgttc accgcctgaa ctggggttta ccactaataa aacttttttc atcactttct
    55201 ttcccttctg attgatttct ttaatataac gatagcataa agaaaaaaat taggctgtga
    55261 tgaagtactg ttcatgaaac attcatttat tttttgacaa gataaaacat ccatactatg
    55321 aaactgagga aaattaaaac aatgatatag gaaataaaag aaaatgatag gcattgtata
    55381 cctaataaag taaaaatctt aggtggcaga taaaaacaca aaaatagcaa gagaatgtaa
    55441 tagcgtttct tccacttttt gttatcagac cttatagcga tcagaggaat accaaatgca
    55501 taactgaaaa actcttgatg tactgttaat atgtcacggc cttgaataat aaatgcgaca
    55561 atccccgcta taattagtgt gcctaacacc aaaacggaaa attgatgaat atattttata
    55621 tacaaatgat ccattgtact tcctgccttt ttatttgtga gaaaaacaaa tccgtatacg
    55681 tgaataactt ctagaaataa aagaaatttg caaaaccact ccatttatct acttctacgt
    55741 gcattttttg atattgtttt ctttatttaa gattatacag aaaaatatct ag
//
LOCUS       DAYYOA010000006        54079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000006 DAYYOA010000000
VERSION     DAYYOA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.006"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..685)
                     /locus_tag="ACHBKA_000334"
     CDS             complement(107..685)
                     /locus_tag="ACHBKA_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9444056.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(705..1373)
                     /locus_tag="ACHBKA_000335"
     CDS             complement(705..1373)
                     /locus_tag="ACHBKA_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444057.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1388..2458)
                     /locus_tag="ACHBKA_000336"
     CDS             complement(1388..2458)
                     /locus_tag="ACHBKA_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444058.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2629..3993)
                     /locus_tag="ACHBKA_000337"
     CDS             complement(2629..3993)
                     /locus_tag="ACHBKA_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9444059.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3990..5549)
                     /locus_tag="ACHBKA_000338"
     CDS             complement(3990..5549)
                     /locus_tag="ACHBKA_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444060.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5605..8085)
                     /locus_tag="ACHBKA_000339"
     CDS             complement(5605..8085)
                     /locus_tag="ACHBKA_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444061.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8069..8404)
                     /locus_tag="ACHBKA_000340"
     CDS             complement(8069..8404)
                     /locus_tag="ACHBKA_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444062.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8418..9452)
                     /locus_tag="ACHBKA_000341"
     CDS             complement(8418..9452)
                     /locus_tag="ACHBKA_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9444063.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9542..10222)
                     /locus_tag="ACHBKA_000342"
     CDS             complement(9542..10222)
                     /locus_tag="ACHBKA_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9444064.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10506..11609)
                     /locus_tag="ACHBKA_000343"
     CDS             complement(10506..11609)
                     /locus_tag="ACHBKA_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM9444065.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(12238..12708)
                     /locus_tag="ACHBKA_000344"
     CDS             complement(12238..12708)
                     /locus_tag="ACHBKA_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM9444066.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            13038..14678
                     /locus_tag="ACHBKA_000345"
     CDS             13038..14678
                     /locus_tag="ACHBKA_000345"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM9444067.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            14832..16154
                     /locus_tag="ACHBKA_000346"
     CDS             14832..16154
                     /locus_tag="ACHBKA_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM9444068.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(16222..16656)
                     /locus_tag="ACHBKA_000347"
     CDS             complement(16222..16656)
                     /locus_tag="ACHBKA_000347"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9444069.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(16721..17558)
                     /locus_tag="ACHBKA_000348"
                     /pseudo
     CDS             complement(16721..17558)
                     /locus_tag="ACHBKA_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(18300..18926)
                     /locus_tag="ACHBKA_000349"
     CDS             complement(18300..18926)
                     /locus_tag="ACHBKA_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444070.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(19221..19556)
                     /locus_tag="ACHBKA_000350"
     CDS             complement(19221..19556)
                     /locus_tag="ACHBKA_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9444071.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(19543..19827)
                     /locus_tag="ACHBKA_000351"
     CDS             complement(19543..19827)
                     /locus_tag="ACHBKA_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9444072.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(19883..21406)
                     /locus_tag="ACHBKA_000352"
     CDS             complement(19883..21406)
                     /locus_tag="ACHBKA_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9444073.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(21399..22574)
                     /locus_tag="ACHBKA_000353"
     CDS             complement(21399..22574)
                     /locus_tag="ACHBKA_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9444074.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(22578..22763)
                     /locus_tag="ACHBKA_000354"
     CDS             complement(22578..22763)
                     /locus_tag="ACHBKA_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444075.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(22729..24423)
                     /locus_tag="ACHBKA_000355"
     CDS             complement(22729..24423)
                     /locus_tag="ACHBKA_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9444076.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(24420..24893)
                     /locus_tag="ACHBKA_000356"
     CDS             complement(24420..24893)
                     /locus_tag="ACHBKA_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM9444077.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(24962..25117)
                     /locus_tag="ACHBKA_000357"
     CDS             complement(24962..25117)
                     /locus_tag="ACHBKA_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM9444078.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(25251..25631)
                     /locus_tag="ACHBKA_000358"
     CDS             complement(25251..25631)
                     /locus_tag="ACHBKA_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9444079.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(25635..25850)
                     /locus_tag="ACHBKA_000359"
     CDS             complement(25635..25850)
                     /locus_tag="ACHBKA_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444080.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(25853..26260)
                     /locus_tag="ACHBKA_000360"
     CDS             complement(25853..26260)
                     /locus_tag="ACHBKA_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM9444081.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(26526..28001)
                     /locus_tag="ACHBKA_000361"
     CDS             complement(26526..28001)
                     /locus_tag="ACHBKA_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM9444082.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(27991..28839)
                     /locus_tag="ACHBKA_000362"
     CDS             complement(27991..28839)
                     /locus_tag="ACHBKA_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM9444083.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(28876..29238)
                     /locus_tag="ACHBKA_000363"
     CDS             complement(28876..29238)
                     /locus_tag="ACHBKA_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444084.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(29239..29397)
                     /locus_tag="ACHBKA_000364"
     CDS             complement(29239..29397)
                     /locus_tag="ACHBKA_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444085.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(29538..30162)
                     /locus_tag="ACHBKA_000365"
                     /pseudo
     CDS             complement(29538..30162)
                     /locus_tag="ACHBKA_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(30159..30470)
                     /locus_tag="ACHBKA_000366"
     CDS             complement(30159..30470)
                     /locus_tag="ACHBKA_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444086.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(30514..30807)
                     /locus_tag="ACHBKA_000367"
     CDS             complement(30514..30807)
                     /locus_tag="ACHBKA_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444087.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            31001..31648
                     /locus_tag="ACHBKA_000368"
     CDS             31001..31648
                     /locus_tag="ACHBKA_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9444088.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            31709..32854
                     /locus_tag="ACHBKA_000369"
     CDS             31709..32854
                     /locus_tag="ACHBKA_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9444089.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(32935..33240)
                     /locus_tag="ACHBKA_000370"
     CDS             complement(32935..33240)
                     /locus_tag="ACHBKA_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM9444090.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(33265..33696)
                     /gene="ruvX"
                     /locus_tag="ACHBKA_000371"
     CDS             complement(33265..33696)
                     /gene="ruvX"
                     /locus_tag="ACHBKA_000371"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM9444091.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(33693..33959)
                     /locus_tag="ACHBKA_000372"
     CDS             complement(33693..33959)
                     /locus_tag="ACHBKA_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM9444092.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(34214..34939)
                     /locus_tag="ACHBKA_000373"
     CDS             complement(34214..34939)
                     /locus_tag="ACHBKA_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9444093.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(35300..35370)
                     /locus_tag="ACHBKA_000374"
     tRNA            complement(35300..35370)
                     /locus_tag="ACHBKA_000374"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35336..35338),aa:Gly,seq:ccc)
     gene            35487..36239
                     /locus_tag="ACHBKA_000375"
     CDS             35487..36239
                     /locus_tag="ACHBKA_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9444094.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            36255..36734
                     /locus_tag="ACHBKA_000376"
     CDS             36255..36734
                     /locus_tag="ACHBKA_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9444095.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(36985..38565)
                     /locus_tag="ACHBKA_000377"
     CDS             complement(36985..38565)
                     /locus_tag="ACHBKA_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM9444096.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(38558..39301)
                     /locus_tag="ACHBKA_000378"
     CDS             complement(38558..39301)
                     /locus_tag="ACHBKA_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444097.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(39477..40253)
                     /locus_tag="ACHBKA_000379"
     CDS             complement(39477..40253)
                     /locus_tag="ACHBKA_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9444098.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(40501..41337)
                     /locus_tag="ACHBKA_000380"
     CDS             complement(40501..41337)
                     /locus_tag="ACHBKA_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM9444099.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(41348..42277)
                     /locus_tag="ACHBKA_000381"
     CDS             complement(41348..42277)
                     /locus_tag="ACHBKA_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM9444100.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            42469..42822
                     /locus_tag="ACHBKA_000382"
     CDS             42469..42822
                     /locus_tag="ACHBKA_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9444101.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(42896..43552)
                     /locus_tag="ACHBKA_000383"
     CDS             complement(42896..43552)
                     /locus_tag="ACHBKA_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9444102.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            43778..45712
                     /locus_tag="ACHBKA_000384"
     CDS             43778..45712
                     /locus_tag="ACHBKA_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9444103.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(45798..47021)
                     /locus_tag="ACHBKA_000385"
     CDS             complement(45798..47021)
                     /locus_tag="ACHBKA_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9444104.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(47015..48886)
                     /locus_tag="ACHBKA_000386"
     CDS             complement(47015..48886)
                     /locus_tag="ACHBKA_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM9444105.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(48907..50025)
                     /locus_tag="ACHBKA_000387"
     CDS             complement(48907..50025)
                     /locus_tag="ACHBKA_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9444106.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(50108..50941)
                     /locus_tag="ACHBKA_000388"
     CDS             complement(50108..50941)
                     /locus_tag="ACHBKA_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9444107.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(50934..51851)
                     /locus_tag="ACHBKA_000389"
     CDS             complement(50934..51851)
                     /locus_tag="ACHBKA_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9444108.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(52078..53202)
                     /locus_tag="ACHBKA_000390"
     CDS             complement(52078..53202)
                     /locus_tag="ACHBKA_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9444109.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            53385..>54079
                     /locus_tag="ACHBKA_000391"
     CDS             53385..>54079
                     /locus_tag="ACHBKA_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9444110.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPAS"
BASE COUNT        16120 a        10935 c         9027 g        17997 t
ORIGIN      
        1 caaatttgta tttatgaaaa actacctata taaaaaaaac aagatataac gatcttcgtc
       61 atatcttgct tcttcaccta actatttttc tccctatata acagctttac gcttctccta
      121 atacaccatt caaatataaa tcgatcaatt gcgccacatt tagcggttct tccacaggtc
      181 ttttcgaccg agtgatgtaa ggtgctaaca ctaagaaaaa atgccttgct gcgatttctt
      241 ttgaaatccc atttcttagc acagattggt ttgctgaaaa gatttcttca aaaggttcac
      301 gataattttt cttccataga aagaaaagat gatctcttgt ttcctttgaa agattgcttt
      361 caagatcgtg aagcatcaga aacaaatcga ccagatgtgt ctcaatcaaa aactgcgcca
      421 tttttgtcaa ggttacttga aatcccgttt cgcttgtttg aacgatttct ctcaaagctt
      481 ttccaacatc ttttaagtgg ttttctaaaa ctgccgtata tagtttttct ttatcaccga
      541 aataatgata gagattgggt tgggtgatcc caactttttg tgcgatctct cttgttgaag
      601 tgccgagata tcccttggac ataaacaagt cagtcgcttc tttcaaaatt ttttgctgtg
      661 tatctttgtt gtatcgatgg accatttttc cacctgcttt ttggttattc tttttctttc
      721 aatacaccat cttccatcat aaatattcta tcgcatttgt caatcagccg tgtatcgtga
      781 gtaaccatga tcgttgcttt atttttgtct tttgattctt ttgccaagat atcgactact
      841 tctcgtgcct tttctgaatc caaactagca gttggttcat ctgctagaat aatggacgga
      901 tcattgtaca atgcacgagc gattgcgacc ctttgccgtt ctccaccaga tagttcttct
      961 ggatacttgt tgatcaactt atccacgcca agctgctcaa ataattcttt tgcttctttt
     1021 tgacgatgtt catgtttgat cttatctact aataataact gcttttttac tgtcagaaaa
     1081 ggaaccagat tggaagcttg caaaataaag ccaatttctt taaaacgtag tttaacgcgt
     1141 tcactttctt tttgattact gaattcttga tcattgataa atacagagcc actgctaggc
     1201 gtctgcaatc cgcctgccaa agtcaaaaaa gtacttttac ctgaaccaga aggaccgatg
     1261 attgcaacaa attccccttt atcgatactc atggttgttt ctttcaatgc tttaactact
     1321 tggtcgccat cttggaaact tttttctact tctttaaatt ctattgcttt catgctcttc
     1381 ctccttctta tccaattgcc ttcaacggat cgatttttac gattgtccga acagaaaata
     1441 gtgcgcctaa cacagctact accagcatca acaagctaat gacgccaaaa aacagccaat
     1501 tgctttggaa tggaaccgca tcaggtagaa caagggatgt tcccactgtt cctaatagac
     1561 caagaagaat accaacaaat gataaaatga atgtttgtgc cactacagaa cgcgcaataa
     1621 atcctcctgt gatcccttgt gctttcatta caccaaagat attaatcttt tgcatcgtca
     1681 gtacataaat gaaaatcccg atcacaatag ccgcaataac gatcaaaaat ccgatcataa
     1741 atccaaaagt caatacttgc gcattatatc caggtaattc attgatgaat ttagaaattt
     1801 tgatctgttc taaatcttca ggtaaattac tgattttacc tctgacaact attgcgttaa
     1861 tccgtgcatt ttcacttgta tcttcttttt cgaagcggat ctcttggtaa gcattgatgg
     1921 ttgtatagag aactggtgag acattgaatt tggcatgatc cgtaaagcca gttagtttaa
     1981 acgtcttgtc actaccagat aacttaactg tatctcctac ggctagacca tattcttctt
     2041 tcaaactaat atcgcctact gcctcatcat cgttatcaaa agcttttcct tcaacaagat
     2101 tgggcatgat gaattgattt ttatcgatac cgaagaagct aacattgatt tttccttctt
     2161 ctgtactgtc tttactagtc acgactccag gtgtctgggc aagatacgcc actttcccac
     2221 cttcgacttc ttctgctatc ttcttagtaa tcatggacat ccctagattt gaatttgcat
     2281 ctttcgaaag cacaatcgaa tctgcctccc acttgtcgac tgctgttcga ttatcttgag
     2341 ctaagccata agctaaccca gtcaaaaaaa ataccaggta ggcgatcaaa aacatcaccc
     2401 ctgcaactaa cgcataacgt aactttgaat gcataatttc atttaatgct aaaaacattt
     2461 aactcctcct cctgaaataa tcattttata caacaatttt atcacttgat aaatttttgg
     2521 cgaatattat gctcaaaaaa ttcttccgct atttcattat atgaattttt tattctcgat
     2581 tgattaatat ttattatatc cccacacaat aaaagaccgt gatatttctc acagtctttt
     2641 atctactcta ttgctatttg ctctcttagc tagtacttta aaagctaact cattgaggta
     2701 gtgaaaacga gaggtgtgtt cagacagaaa tccaaaatcc tcaactgcta gctgtggaat
     2761 cgtagttttg tgtttcacga tatccgcaag caggttcaaa tctgataata agaattcttg
     2821 gcttatcagc tgtccgccaa ttatttctaa tgtatccgga tggtagatca gctttgcatt
     2881 tactgttttg ttttcttcat cgtattgtgg aaatagatca aattctcctg aacacgaacc
     2941 ggtattttgt tccaagaatg gcgcttcatc ttctgtcagc cctacagttc ctaaaaagta
     3001 gtctataatg actgctgctg ttatcttttg tctgaggtcg taagcggttg gctgaccact
     3061 caaagttagc gctactgctc ttgccatatg tacagctcga gtaactaaag gtaaaaagag
     3121 atgccgttta tttttgtcaa aactgactgg gacgatatct ccaatcgcat agatgtccgg
     3181 atcacttgtc tgcagatatt cattgactat cacagtctca tcctcataca aagccacttt
     3241 ttcttttaac aatgtcgtat taggacgagt ttgggcagaa aaaatgacca tatctgcctt
     3301 tattgcttga gatagaagtt gtacggatac tactttctct tctgattttt tccaatcgat
     3361 caagaattca tttggataaa aattgatttg cttagtttgc agctgatttt gaaaactgtt
     3421 gatcatatcc ttatcgaaat atcttgataa aacattcggc atataatcaa ctagatgaat
     3481 aatgtatcct ttatttctca aagcatctgc taattccaat ccaatataac cagcaccgaa
     3541 gatcactact tcttttgctt cttttaaagc ggcaagtact tgcttagatt gcactagatt
     3601 tttataggtg tagacatttt cagcttcaat ttgtttatac aaggtgacag gagaggaacc
     3661 tgttgctacg attaatttat cataggttat ggtctcgttt tttccttctt tggatgcgag
     3721 gatgagctga tgtttgtctg gatcaagttc agttactctc cattcggtga tcagttttgc
     3781 cccactttct tgaagagtcc gcatgttatg ggcaacttca gctaaactac gtatctttcc
     3841 ttttaaataa aggttgattc catttgaaac atagcctagt ccatctttat gatcttcatc
     3901 gatcagtaaa acttctgctt tcggttgaag ttttcttaga tttagggcag ctgtgatgcc
     3961 tgcatgggaa gcaccaatga tcacgatttt catgttttat tcatccttaa attcaatttt
     4021 tctaaataac attcttttaa aaattggttc aaatagggga aatcataatt atattcgttg
     4081 ctagtgaata caaaaacttt agcggacttc tgtgacgggt actctttcaa ttggctagtc
     4141 aaaatgatat catactgctt ttctttctcg tattcgacta aatcatattt gatcgtattc
     4201 agcaattgtt cctggatgat ttctttcacg atatccgcca ttagcctttc ataaggaaga
     4261 tcataagcaa tttgcagacg taattctaac tgcttgatca gcgtccgcaa aatagatgca
     4321 tagcgagcaa ataattcaaa gcgggcattt tcctcgtcta cttcagtaaa atcaacaata
     4381 gcaaatgttt gtctgaccaa tctttcacat aaaaccagga attgtttatc ttctacttca
     4441 gttaaccgct gttttaatcc ttcttggcca aaaatattca tccatcctct aaaataggca
     4501 aaatgagcgt gattttgcat cactgtcaac gcaatctttt gcgaaatctc ttctggaaaa
     4561 acataaaaag agaaatattc ttctatcgat tgtttaaacg ttcgattcaa ttgagctact
     4621 acgtcgtctt tgtatttttc gcttatcaaa aactcctgga catgcgagtt atgtgtatct
     4681 aatgtaaagt tactgatcat gaaaagataa aaaaccaacg tttcattttg attccaagga
     4741 aaggcatatt gaaagaagta acgggtcaaa aatgtatgga ggtccaataa aaattgtgtc
     4801 gtttgaaaca atccaacagg aagattgcta taatctggtt tggatttttt cgtacagcgc
     4861 attcgcttct tcataatgga aagccatatt tcaaatttca atcgtccttc ttctgtaata
     4921 gtaaaactaa actctttttg cagcagatct aaaaaagtgt ttcctgataa atcactgtat
     4981 tcttttttgt tttcaagata aggacgtcca aaccaaaata attggaaata aaaataacaa
     5041 atttgacttt ctggaccggt cagccgcccg ctttttattt tcagatgaaa ctcatttaaa
     5101 gaatcattta attccttcac tctgcgagac aaagtagccg aactgatatt aagttccatg
     5161 acaagtttct gccgatcaaa tgagccattt tccaacagat aaagaagtat ctgatatttt
     5221 gtagaatagc gcaagtagca atgataataa acatcgactg ctgctatatt tccatgtctg
     5281 attaataaaa tattcccttc ttggataaca atttccaatt gtttgatatt ttccaagtta
     5341 gcttgtaaat aaaacagatc ttctttcaag gtagcccgat caactgataa gcgggtaagt
     5401 acgttattaa cggaaatctc tcctttttcc tctaataaaa gttggaaaag tttgatttgc
     5461 cgttgttcat ttttttcaag caaactgaaa agttccaagt ccactcctcc aagcatttct
     5521 gattttaaca gcagaagttt ccttctcatc cttgaaaaag aggatctgtg acagttatca
     5581 cagatccata caaattctaa tcacttagct ggttggccca gctgtcagtt cccatgtgac
     5641 catgccttga tacgttgcgc caggttgagc attcgtactg ctatttacct ctaagagtaa
     5701 accatcggta tcgggaaagt caacgagaac ctctccttta gctgttccat cactctcata
     5761 aatcaacgtg ttttgacctt gctgaatcaa ttgttttgat ccagaatgat tgaagtataa
     5821 ttttgttccg cttaattctt gaccttgatc attcttaaat aaagaagtca atgtccctgt
     5881 cagttgccaa ggagacgtgc ttggaagccg agtatcccgc acatcaaaag aaaaatcgcc
     5941 ttttgcttca taataacttg tcttcaacgg tttggggaga tcgttaccaa atgtaaaatc
     6001 atctggtaca ttaactaatg ttaattcttg tttggggaca agaattgttt tagcagatgt
     6061 ttggacaact gggttaggaa gcttagcacc atctgctaat gtaatattgc tggaaagtac
     6121 agctgccttt gtcagtatgc tgacggattg tacagaagta tctacagtat ttaattgata
     6181 gttgtaagta agatgaatgg tatctcctat tttgctcaat agatctgctg ggacagaaac
     6241 tccttgttga atgccggtat ctgcgatagt tgtaccattt aattttaagg aaccattgat
     6301 ataagtagta ttttcaggaa gtgagatcgt gatttttttg tcggttatcg catccccaga
     6361 ttttgttaac gtgaatgtca cattttcttg aatcgtatct cctgatagag cagacagggg
     6421 atctcctgtc tcaggttgtc tgctaaggtt ttttagctga ctatctgcac tcacagatgg
     6481 aaaattagcc actgttattt gaggaagagt ggtgattcca gcttgttcag actgccattt
     6541 gtacgcaaaa gacggataca tgttagtaag tacatcgtcc accaagcgat aatcaaaata
     6601 atattgccct gaagccaatg caggtagtgt agaagtagct gtatttacac ccccgttatt
     6661 tgattggatc gtttggaaaa gttgatctgt ccatcctgtc gctaattttt gggttccttt
     6721 cgaataaagc ctgtaatgga tcgaaccctt ttttagttca gttgctgacc aattggccgt
     6781 gacttggtac gatgcagaag aaaggagctt tccccactga ctatcggctg ttaagtttgt
     6841 tggtaaaacc gttccgtcta gcttgtactg ctccaatacc tttaaagcaa caggagaaag
     6901 caatccgcct tgcgcatctt tcccactgta cacgatattg gctgtctgat tcaatccact
     6961 tgtgcttgct ggagtatgag tcagtattgc tccttgtgta acgttttttc ctgtaaaata
     7021 gttgactacg cgatcaaagc gactaaatgg caactgatca aaacgtcctt cagtcagccc
     7081 catcgtcaat gtccgactat tggtagagcc tggaaggttc tggttatttc catccacgta
     7141 ttctgtggta tcagcaaatg tattatattt tcctgttgaa tcacttgttg agatgtaact
     7201 attccacgta tttatctctg cacttgcaga aaatgcatat gctcctggat tcaagtttcc
     7261 gccagttaat ttaggataaa atctacctgc agctggagct aaaatcgaat tgaacatacg
     7321 atcttggata atattattcg aatcataaaa atcaacagaa aaaacggcct ttttagacat
     7381 agaaggttgt aatttaccct tggtcaaaat tttgtttgga ctcgtatcat atttatctcc
     7441 gttttctgtc atcttcgcga cattagctgt aacatcaaat tgaagcggga tatcgtccac
     7501 gttgaattcg cctcgcgtaa taatagcaat aatatctgcc aatagagtat ttgtatccca
     7561 cgaccccatc ccaaaacgca aggtatggct tcctgctgat aataaactac cgctagtaac
     7621 cttgatgggg gcaccggcaa tatacaagta ggcgttgttc cagtcaattg cttttaatac
     7681 atcactgctt gatacatgtt ccggcaaatt gatgatcgcc acaaatgcaa atccttgtgc
     7741 agtgatattc accaaatcaa gtacatttgt tagtttcaca tggctttccc aatgaaaacg
     7801 gaccgtgcca cctttccaag gatcatttga accatcgttc aaataagtag cagtcaattt
     7861 aggcaattgg ttagccccag ttagttggac aagcttcccg ccattgactg cactacgtcc
     7921 accatttgga ggtacgatat tttcatacgt gtatggatca taagaaatgg caggaggcgt
     7981 tgctccaaca gcttctgccg aaggtgaaaa gataaatata cttaaaacaa aaagcgaagt
     8041 aaaaaagaag gagcgaaaaa atgaaaattt attttgtagc aacatgttca tcctcctttt
     8101 cctttcttct ccataacaac aaccacgcaa gaagccataa ttctatacca atgatcaaat
     8161 aaagaaaacg gatcttttct tcattcgtct ccgggaatac gccatgttga ttagattctt
     8221 tataaatcgg tagctgtgtc tgctgttttg atgcgccatt gactgaacca cttggatgcg
     8281 gaactggact tagatcataa aatgtaatcg tacctgaact tttggtatct gcttggactg
     8341 gctcttgcca gctgaagaac agcaaacttg caataatccc tcccaataag atcgatgttt
     8401 tcaacgaaat cacctcatca tgttgtttct ccttttttct ttttcttgtg gacccacaaa
     8461 aacagcaaaa tcaaccataa cactagaact gcagcaacca atcctgcata aagccatggg
     8521 aaagattgtt cttgtactgc aaaaacggaa ttttcattta ctttttgtgc tttttctttt
     8581 ttgatcgtaa agttttgtgt aaatcgccat tcattactgt cgcctgattt tgctatgatt
     8641 tctgcgcggt aatcgccggc ttttgcttta tcaccagcta aatcaagatt caagtgaaaa
     8701 ttcgaatttg gtgcaactaa aaattcatta gacgtatttt caatataaag ctgattttca
     8761 taaaagatct tgctcgtaaa tatcaaattg ctgataatcc taggctgagg attttggaaa
     8821 cgaagctgaa cagttggctt tgcgttgacc tcgtccaaat ctcctgataa aagtttcaga
     8881 tccggttcta tcgtctcttg actttcttgt aaaacgactg cgagattata agcaatacga
     8941 tttttgattt gtgctccttc tgtagcattt tctgtttcag cttcttcttt aggtgaaaca
     9001 tgaatacccg ctaatacacg tcccttaaag ctttttgctg ggattttcac ttctataggt
     9061 atagttgttt ctgcatgtgc tgatacattg atttcctttt tcgcaatgag caaaacgtca
     9121 cggacatcaa acggcaatgt ctcatctaac gttgggtttt tttgactata atcgatcgtt
     9181 ccaccatcac tcgttaccgc cggattgaca gagacctcaa atgttcgttc ttcatctgaa
     9241 gaattgctga taattacttc tagtgtctga ttttgttccg gtgccactaa taaatcaaaa
     9301 taaccgattg ctttatctat ttggttctct ggaaataccg gtttgattcc aaagtctcct
     9361 gctgaatcct ttgcttggct gattgacgga aaaatgaaaa atagacttaa aatagaaaaa
     9421 aacgaaacaa acagtcgttt actttgattc attgtccacc acatcctttc aacaacaact
     9481 gcggtaaaaa atccttcacc tatagtattt tcaccgcagt ttgttgaatt tatttcatac
     9541 tctatgctac tgggccagcc acgaggttcc aagtcaaatc agccgtgaat gttccagctg
     9601 ctgctttcgg cactttcagt gtgatatttg ctggatcata aacagaaagc catgttccca
     9661 gaccttgatc ttttgctgct gtaaatattg gtgtcgctgc acttgcttta ctcaacccag
     9721 ttacagtttg actagttggt ccagtggatt cgttatctga cgtactttta acagtggatg
     9781 ttccaaaatc taattcagcg ccttgcaaaa cttgtgaacc gtttacaaaa tctgaaaccg
     9841 aaactgtcac gttccaccct tgtgcttggc catctgcacc tacacctcga cgatctgata
     9901 cttggagata aggtgtatcg ttttttgaag cgtctacttg atacgttttt gttccgcttt
     9961 caatctcgtg tgtaccaaat ggcaattcag gaactacatc caatgttaaa gaacctgtct
    10021 ggccagttgc tggattttcc ggatctgctg gctctactgg ttttgaagga tcagtagggt
    10081 taagaggctc aacggtattt gttggttcag taaaggtaat atctcctttt gaagtcgctt
    10141 gaccctcagc aaaagctggt gttgccaagc cgcctagaag aagtgttgaa agtaataatg
    10201 tcccaatcat gttctttttc ataataaatc actccttttt cttagtgacc taagtatagt
    10261 agagacagta tgcaaaaaat cctcatgaat tcatccgctt acttgaaatt atgacccttt
    10321 ttgtataaaa gagaaaaaaa caataaaatc gtttacatga atattcttcc ttttttatta
    10381 agtatgaaaa aatatttcaa ttttattttt tcgtttcaaa ttattctatt agtgacatcc
    10441 ttgaactata aaaataggag tacggcatca tctgccgcac tcctattttt ttcgtcattt
    10501 atttcttaaa taaaacgatt ctcacgcaat tttatttttt tgatatcttc tactgtttgt
    10561 gttccagcta attgcatgac catttccaat tctgttttaa agaaatcaaa cacttgatgg
    10621 actcctgtac ttcctcctaa agacaatcca taaataacgg gtctgccaat agcaacaaga
    10681 tcggcacctg atgcgattgc tttgaaaaca tgttgtccac gtcgtacacc gctgtcaaac
    10741 acgattggta cacgtttatc tactgcttct gccacatatt gcaacgaatc aaacgcagca
    10801 ggccccccat ctaattgacg gccaccatgg ttagataccc aaattccacc agcaccagat
    10861 tctaatgatc gatagacatc ttcttctgat tgaacacctt tcacgtagac tggcaaatca
    10921 gaatgagcag cgataaattc tacatctttt ggactaagtt tttgtttgga agatttatat
    10981 acagcatcca tcgtttggcc tactcctgat tgataagcct gaacgatcgg cattggcagc
    11041 gggaaggtaa acccgtttct gcggtctgtt tcacggtttc cgccaactgt tgcatccgct
    11101 gtcaatacga ttgcttttgc tccattacgc ttggccactt caagaatatc caaattgatt
    11161 ccattatctt tgctcatata aaattgaaac cattgcggtg ctttctcccc gcctgcttct
    11221 cttatctctt ctaaagtaca agaagcgtaa gaacttgctg tatagatcgt cccaaaatct
    11281 gctacacctt ttgcagaagc tttctccgcc ttcacatgag ctaatccatg agcagcaact
    11341 ggtgccatga tgattggtgc agtcagcatt tcttcgtcaa aatgtgtagt tgtatcgggt
    11401 aattctacat ctcttaatac atgaggaata atcaaacgat gattgaatgc ccgttcattt
    11461 tcttgataag taaataagtc gccagcacca ctgctgatgt aaccataacc accagctgga
    11521 atcacttgtg aagccgcgat ttctaaatct tccatattga taaaatctac tattccttca
    11581 tttgtacctg cttgatatgt tttttccatc tcaatgcctt ctctctataa aatttgaatg
    11641 atgaacaata tgatagcgtt tacacttttt atattactct tcgacattga tacaggcaaa
    11701 taatctgttt acatttcagc cattccagat gtcactactc tggaagaaat gattgtaata
    11761 aaaatagata aaaaaccgat gggactttga caaaagtagt gtcaaagtcc ctttttttga
    11821 ataaacggtg ttcaaaagcc attttctcgg tattaagtca aatttatgca aaaatgtgga
    11881 aagccatttt cacaaaattt ttcttactac tcgaaactaa gcgacttttt cacaacctct
    11941 cgataaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaatg gcaaaatatg
    12001 aggaactatt ttgccatttg tctctctaat actcaggtca gaacgttgac ttcacagtct
    12061 cttccttaaa cggtgttcaa aaacttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    12121 taggaggagc tatttttgtt tggctgttct tattacttga tgcagggcag gtttttcacg
    12181 acctctgatt cacggtgttt ctttttcatc aacattttta cgaaatactg tggaagacta
    12241 ttgaaataag tgtggattgt ttctttgtgt ttcaatcgtt tcttgccatt cttcatttaa
    12301 ttttggatta ccgttttctt ccttgaactc ataaggtcct aattcaaaaa tcccagacag
    12361 tatttgattc acgatttcat atagttcagt gtgatcagac acatgattct tgaagatcat
    12421 ctcattatca ccacgtttga acctcacatt cgggctctca tgatgaacct ctatgcattt
    12481 ttctcctttt tttaactgac ggacttctga aaacatctca taaatatctt ctccagtaaa
    12541 accttcagca agcgccagac tgtccacaag accgcctact gtcagttcag agattattgc
    12601 ttgattggaa tctaatttta atgtatagct tgtcgcttct actacgccac atgcattgca
    12661 accgccttct gatagttcgc tttttctaat caattgaact tctttcattt tctcttcctc
    12721 ctcttaattt tctacttatg tatttttatc acctgacata ggtgactcct atcaacgcta
    12781 tcatgtgctt ttttcctcag aaatcctatt tatccattcc atctatccct tattcttcta
    12841 cttttctttc tctagcatat aagagatacg catagtattc aagcaaggat tgtcgatata
    12901 aaaagaacaa atactgctat tttgatttct tttggctata ttacatataa ataaaattta
    12961 tcatacctgc tctttttgac tatatcatca tctgacttct atacttcgtt tgtattaaaa
    13021 cttaggagga atcattcatg cttacagggt taaacttatt aaataatcct tttctaaata
    13081 aaggaacagc ttttacaaaa gaagaacggg caaaatatgg aatcactggt atgcttccaa
    13141 gtactgtcca aacactggaa caacaatctg ttcaagctta tggtcaatac ttaagcaaac
    13201 aaacagattt ggaaaaacgt atcttcttaa tgaatctatt caatacgaat cggacattgt
    13261 tttataaatt gatgggcgaa catttagttg aattcatgcc tgtcgtttat gatcctgtcg
    13321 ttgcggattc aatcgaacaa tacaacgaaa ttttcttgaa accgcaagat gctgcatttc
    13381 tttcaatcga cgaaccagaa aacatcaaag caagtttgaa gaacgcggca gatggacgag
    13441 acattcgttt gattgttgtg actgatgcag aaggaatctt aggtatgggc gactggggtg
    13501 tgaacggtgt tgacatcgct atcggcaaat tgatggtcta tactgctgct gctggtatta
    13561 atccagcaca agttttgcct gtatcgatcg acgctggtac taataatgaa gaattattga
    13621 ataatccctt atacttaggc aatcgtcatg ctcgtgtaga aggcgaaact tactatgaat
    13681 tcatcgatca attcgttcaa tcagctaccg aattattccc agaattgtta ttgcattggg
    13741 aagattttgg acgaggaaat gctgcagcga tcttagaaaa atacgaagat caaatcacta
    13801 ctttcaacga cgatatccaa ggtacaggaa ttgttgtcct agcaggtgta ttaggcggat
    13861 tgaatatatc cggtgaacaa ttgaaagatc aaataatttt gacatttggt gctggtactg
    13921 ctggtgtggg tatcgcaaac atccttttag atgagatgat ccgtcaaggt gtacctgaag
    13981 aagaagcacg ccaacatttc tatcaagtag ataaacaagg tttattgttt gaagatacag
    14041 aaggattgac tcctggtcaa atcccattcg ctcgcaaacg ctcagatttc actaatgctg
    14101 atgaattgac aaacttagaa gcagttgtaa aagcgatcca tccgacaatc atgattggta
    14161 cttctaccca acctggagca ttcactgaag aaatcgtgaa agaaatggct gctcatacac
    14221 cacgtccgat catcatgcct ttgtctaatc caacaaaatt ggcagaagca aaagcaaaag
    14281 atttaataga atggacggac ggaaaagcat tagttggaac aggtatccct gctgctgatg
    14341 ttgaatataa tggtgtaact taccaaatcg gtcaagcaaa taatgctttg atgtacccag
    14401 gtttaggact tggtttgatt gcttcaacag ctacacgagt aaatgctgaa atcatttctc
    14461 aagctagtcg agcattaggc ggaatcgttg atgtaacaaa accaggagca gctatcttgc
    14521 ctccagtagc aaaaatcact gaattttctc aaacgattgc tgaaactgtc gcaaaatcag
    14581 ttgtcgcaca aaacttgaat cgtgaagaaa tcacggatat caaagaagca gttgaatctg
    14641 ctaaatgggt tcctgaatat aaatctttag aagattaaga tcgttatagt aaaagaacat
    14701 acagaaactt tgtatgacgt ctaaaaagaa aaaagcgact gcggcaaaac agcggccagc
    14761 caactcctgc tcttttgtcg cttttctttg gcttttttca tatgatacag aataagaaag
    14821 gatctgtaaa aatggcacaa aaaacaacaa ctgcaactgc agttgaggaa aagaaaaaag
    14881 aaggtttttg gtccacaaaa atcaccggcg taagtttgcc actttatttg atcatggtcc
    14941 ttgtcctgat cattgcaatg gctttaggga aattaccgac gaatatgatt ggaccaatcg
    15001 cagttttggt tattttcggt aatttatttc attttatcgg gacaaaaatc cctatcgtca
    15061 aaagttattt gggtggtggt tccgtttttg cgatcttcgc ttctgctgcg attgctacat
    15121 ttgggatctt acctgaatat gtagtgaaat ccacagaaaa ctttgtcagc aatatgggct
    15181 tttgggactt ttatattgct gccttgatta ctggtagcat ccttggtatg aaccgtaatc
    15241 tattgatgaa agcttctgtt cgttttattc ccgttgcact tatttcaatg gtcgtctcct
    15301 tttttgccgt aggactcgtg ggcatgctta tcggaaatgg ttttgcaaat tccgttttat
    15361 acatctcatt acctgcgatg gctggtggtg ttggagccgg agccgttcct ttatcaacga
    15421 tttatgcaaa tgttttaggt acggatgctt cttctatcat ttcccgcctg attcctgcga
    15481 cagcaatgac aaatgtctta gctatcatcg gtgcagcatt agttgctcgt gctggacaat
    15541 caatgccaaa attcaacggc aatggtaaat taatggaaaa aggcgatctt ggcgatggaa
    15601 aacctcgaac tgtgaaacct tccattccac agttaggtgt tggtttgatg gtatcattga
    15661 ctttcttcat cttaggacaa attggtaact attttgttcc taaagtccat gcttacgcat
    15721 ttatgattat tatcgttgtc atctgtaaaa tcgccaacct attaccagag tattatgaag
    15781 atgcagctat catgttcaat aacttgattg tcaaaaactt gacagcagct gttcttgccg
    15841 gtatcggtat tgcgttgctg aatctgaatg tattagcttc tgctttgaca tggcaatttg
    15901 ttgtcttatg tctgacaagt gttatcgcga tttcagttgt ttcaggattt gtcggtcgtt
    15961 tgtttggttt atatccaatt gaatcaacta ttactgctgg cttatgcaac aactcgatgg
    16021 gcggaacagg aaatgtagcc gtcttatctg cagctaatcg gatggaactt attgcttttg
    16081 ctcaaatggg caatcgactt ggtggtgcca tcgttttgat catctctggt ttcttgatgc
    16141 aattactttc ttagtcaaaa aaacaaaaat acctgatcat caagtatcgg cttggtgatc
    16201 gggtattttt ttgataaaga tttacaggat tttcaaaaat cttttttgca ttttatcgga
    16261 atgataaccg atgtgttcat aaaattggtg tgcttctact ctgtattctg cagaattaag
    16321 ccttattgtc gttattcctc gttttttcgc ttcttcttct acgccagtca tcaaagcctt
    16381 tccgatgcct cttttttcag catatttaga cacagcaagt gccataatat tgaacattgg
    16441 atcgctaaaa atcgattcat aggactccgc ttgtacatag ccagctgctt ttttagaagt
    16501 aggatcttca taaaccaaga aaaaattttt ctctggctcc ttcagcagtt tcttcatttg
    16561 tctcaccgta acggttaatg gtatatcata ccctaattga tccgcattga tttcacgtag
    16621 tgcttctgca tcttctaaac gcatttcccg taacatccaa atcaccagcc aatttctgat
    16681 tttttaaaat gaattttttt atcatagtat actctttttt tcatttagaa caatatgttt
    16741 cagtgatttt gacttgcgtt agtctagatt ctcctaatgt ttgtaagatg acaaaggcat
    16801 tgtaacaatt gaccaaaaga accacgagga caattacttg aaaaataaag gtcacttttt
    16861 ttggagacaa aaccctgcta aatcttgctc cccacaaacc tccaataaca gcagcgggga
    16921 taacataaaa caacatagat aaatcaaagc gcataaagct acttgttaaa gcaatcgtta
    16981 ccaattttgc cagttgagaa aagaaaatcg tagatagaga atatagtgtt gcttctttta
    17041 ttggcaatgc aaacatcagc atcaacaaag aaacattgac tggtccacca ccaataccta
    17101 aaaaactagc taaaaagccc agaacccccc ccacatataa ggtaccaagt ccatgacgtc
    17161 aaatgaaatt ttggctgatg atgcttggtg tagaagaacg caaaaaccaa ggtcaatacc
    17221 gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt caaataactg taaaaaaact
    17281 tcgaatacaa tgttgccgac tactcctcca acaacagctc cactagatac ccaaccgaca
    17341 atctgccaat tcagtgattt cccgcttgat acttgtcgca cagtcgaaac aatggacatc
    17401 gtgaataccg caactgttga gtaaaaagaa atccctgcaa cagaatgggc gccgataaga
    17461 tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc aactgtgttc
    17521 gccagaacga tcaccacaaa ataaatcaga ccaatcatct tgtatttcct ttctcttgtt
    17581 tatatctttt agcgattgtt actcttttct tttattatac agtaaaagaa acaaacgagg
    17641 cttcgaaagc ttttctactt tttaaggaaa tttagtgcta aacgaaaaga ggttgtgaaa
    17701 aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagctcctc ataattttcg
    17761 tttggttgaa cttattaccg cagatgcaag cttttgaaca ccgtttaaga aagaggtcgt
    17821 gagcctgccg ttcagctccg agtcacaagg aacaatttca aaaaacagct tctcatgttt
    17881 gttgaatatt gtgacttgtg accgaggagt tggcaggaga acaccgttta aggaagagac
    17941 tgtgaagtca acgttttgac ctgattatta gagaaacaaa tggcaaaata gttcttcata
    18001 tttcgccgtt tttcgagcga atgccgaggg gcagctgatt gaacatcgtt tatttggaaa
    18061 aagggattgt gacaagacgt ttgtcacaat cccttttttt atatcttatt aattcattct
    18121 tttgttctat tcttcttctt ccgccatgaa tgagaaaccc atatcaaaga actataaagg
    18181 ttatcaaaac agcgtttatg actaaatgaa ttagagttat ctatcactat ttttcaagcg
    18241 atttaaacca aatggcatac aatatggcat acaaaaactt caataagccg tgggggtttt
    18301 taaatttctt cttgttcttt aaaatcaatg agtggagcta cataatttga atccactttt
    18361 tcaaacgtca ctttttgcat cgacgaatgt tttctttccc aagataacgt aaatagattt
    18421 gactctggac ttataaagtc tacttcacct tctagtatta ctttcttatg caaatattcc
    18481 tctaataaat atagacttgc taaagcatac aacacatttt ccaaatttgc ttccttataa
    18541 tttgaagaac gaccatgttt tactctgtta tatattttcc accaatctgg tgtatcttca
    18601 ggccacccat ctccaaaagg ttttagtgaa gtcaatgtta aagaattcat aaccctaaca
    18661 ccattatcca aacacattaa ttcgctagat ttaataacag ctttaaaatc agttatgctt
    18721 ttattgctac tgctatcaaa tccgcaaagt tctttgaaaa taatctcaca ctccgaacca
    18781 atcgctagta gtagtttttg atattcaata gagaatgttt tgaaattatt ctcatcgagc
    18841 atcacaaaat ctgtggtatt aataaactca ttttctaaat gtagataata cttccaataa
    18901 aaatttgtaa actctgcttt gtccattaaa tcacctcttt tgaattataa caaaaaaaac
    18961 acgtccacaa cagacgtgtt ctttgatttc tacacaatca gcttgccaaa accatccaat
    19021 cagtcctagc atgcctgtat taaaattgaa cgttcatcat ctatcatctt tcttagtcca
    19081 gcatgtttgc aaccacacct acaattttca gtccaacaat agatttcgac tagctaagtc
    19141 ttgcaaaatt caggcagcta acccttcatt tactacctta attatactaa atacccacaa
    19201 cgtttacaag tgtaatcatt ctaagaaatc tgtttagcta tgatcgttgt ataatcttta
    19261 aacgccgttc cttcattgat tgaaataatt ttataactct tgtttttcca agctactttc
    19321 atatcgtttg ttagttcata atcttgatcg tatcgaataa taaacgtcaa cgttccttca
    19381 agaatggtac ctacactagc aagcacgtct tttagcattt gtgaacgcac attagcccaa
    19441 cattcaaata cagtttcttc actatccacc atctgcccat cttcatcttt tacccgtttt
    19501 gtctttttaa acttgactct ctcagtcaat tcatttacat tcttaatgat tggcataata
    19561 agcacctctc atttgttgta ctaatgcttg taccgttact ggtatccgtt cgctgctggc
    19621 ttcttgacgg ttcaaatacc aatgttgagt aagtaatgac accgccaaat caaattgctg
    19681 ataattatcc atatctttat ccgttgcgct actatcaata gcacttttta tatattcgcc
    19741 tgctgtatca atcaattgtt gaataaagtc atcatcaata gtgtgatcga tccgcatact
    19801 tttttttatg ctatccaaaa ttaccatttt atctctccta atgaaaaaaa ggcagtggga
    19861 taaaccgccg cccaatattt ttctatactt ttggtgttac aactggcgta aactcaatat
    19921 acactgaagc attttgatca attttcttat aatcgttgcg aacgatcact gcaagtcctt
    19981 gtgagtagta atcgaatttt tcccattgag tcgttacttg atttctacga gcaacaaaga
    20041 tagattgtgc aatatccccc ataatcatag ggaatgttcc agctttagga ttggcaaaca
    20101 atgtatctgc aatcaatact actggcatac cgaataatga tttaccacta ggtgctgtca
    20161 catcgggttg taaaatgtaa cgtccatctg aatctttcaa tgtatctagg tggtaatatc
    20221 cgctttggtt tagaattacc attttattta atgctgggtc taatgccaca ttgtatagtt
    20281 gtttcaaatc gtccaacgta gcagccgttt tcttagtgaa tgtttttaat aaatccatga
    20341 tatgcttatt gtcagtgttt tctaccaatt tcgctaactg atctttgacc tcttgtacaa
    20401 tattcactgc tgaatcttcc acaacctcat ttgataaggc aatcttacca gcacgagttt
    20461 ctactttata atcaactgaa gtaaacattt cagcgtcaat atcaccaatt tcagcaagtt
    20521 cagcttttgt tgctaacact gcttgttggt tagttgctac tggatattta ccttgtccat
    20581 ttgatactgt ttttactgtt gcatattgag ctaagttata atttgaacgt ttcaaatcaa
    20641 agacttcacc gattacttct tcgggaacaa ctgccgctgc atttacagta gtgactccat
    20701 cacgcacttc gccttgtgaa cggatatatt cttcataccc acgcaattct gttttttcgt
    20761 tatcgattaa agttttttcc atgttattac tctccttttg tgctttagaa gtggattcta
    20821 agctattgtt tgtttgtttt gcttggttgt tactcataaa cgattcgtaa gaacgcttat
    20881 ttacttggac atttgatgaa tcataagcgg gaacggtcac gacactaatt tcattaagtg
    20941 ctttgatttt atctattgat cgttcaattg tgccatcttc ttttttgtcg aatgaatcta
    21001 atccaagcac gaacccaaag gacatagaat caacaacccc ttttgagata ttctcataca
    21061 catcatttga ataggtcgtg tcggttagct ctgcttcaaa atgtaatcct gtatcatcga
    21121 cattcaattt caacgtacct gctttgacac tagctagtgg ctttgaataa tcgtgtccat
    21181 ataataagaa cacatttgat aaatccacct cttttaatgc ttcgggtgta atcacttcta
    21241 caaagccgcc taaatctttt gacggttgcc cgaatttcaa cgcatagcct gaaatagttt
    21301 tgccgtctgt tggttgtttt tcattttctt tctctacttt gggagaatcg gctgttagct
    21361 cagcttcttc tgtcagccgt ttttcctttt cttcttcatt attcaactgg tgtaactcct
    21421 tcctgttctt ttaattgata tttctccaac gtatctaaat acgtaaagtt caaacttgct
    21481 agtaaacgat cgccaccatc catggggggt agccccattt tcgaccgccc ctcattaatc
    21541 gtgagaagtg accctttgac ctgatccaat acatttttta cttttgtttc aggatcagtc
    21601 tctagtaact gatcggaatt aaagcggata ttggtttcaa gtttcgtatc taattcactc
    21661 acaaacacat taaagtaatg aatcagcgta ctttggatat actggagatt actttgaacg
    21721 gtgctagaat gttcattttc aacgcctaaa cgctcaatcg gtacaccaaa cgccttggca
    21781 atttgttttg tgttccaatc attggaattg ataagcttca aaacatcggt gtttatttct
    21841 aatgttttgt agtccattgt ttcatcaaga atgatcgttc taagagcatt atctccacta
    21901 cttgaaccgt tggcttcttc aaacttttca cgaattgcgt tctttgctga tccgtctaaa
    21961 tctgacttat gaaccttcaa aatacctgac ccactgacac cacgagtaaa gaaattgtgc
    22021 aatgtgcggt tgccagcttg ttgaattttc agttcatcac gtaacgcata aagtggtggt
    22081 aatcctgtta aaccatcttg ggtgaaatat ttgaagtgca aaatatcgct agacttcaca
    22141 cgtctttttt tatcaccaat ctgataagac aacgtaccat tatctaattg agtaaccgtt
    22201 acacttgagt taggtaataa gtgaatttct tctacctttt caccgttgcg tttaatctct
    22261 gcaaagctat taccgtttag caacatattg accgccaaag caaactttaa atgccaacca
    22321 tccatttctg aattaggttt ctcgtttagt aacttcacca gttcattatc agcttgtggc
    22381 atattgtttt tgaccaattg aatcggactt gaagcaatat cactagcaat aattcgaacg
    22441 gctgcaaaca catcactatt tcttaatgca cgaacgcttg taaagctggt ggtgtaatca
    22501 tccgattgga ttgataccac atgttgtagg aaaggatctc ccgttgtatt ttgtctcgct
    22561 ttaaaaaatg ccatctttta acctcccttc tgttctgtat ttagcatgac cgctacccca
    22621 ataagcacta tgcccagcgc taacaagcca tagtagacat ttgttagtaa ggtgatagca
    22681 accaacacac acaccaatcc aagaagtaaa agaacggtat gaatgtaatt agaaagaaaa
    22741 ttcttcactt gtataaaatt cattgtctgc tttcgctcct tccgttgtgg tgaagtaatc
    22801 cattgcaaac acataggcat tgattaatgc agctattgga tcaattttgt tactgttctt
    22861 tgatttatta atctgaatgc cgttgttatc cgtttttaaa atagcgttgt tcactgcatg
    22921 ggtgagaatc gtatttttat tgtgaataac gttgccttca taaacttgtt ctctaaaagt
    22981 tcgggtcgga acgtttagag taatcgttcc ttgtcttact tctaacattg ggtagttggc
    23041 tttttcagct ttactgataa gcgaattagc gttatacggg tcataacaaa tccccataca
    23101 ttccaaatca ttttcttgaa tcaaatcttg aataaactga aatacttggt catagtccac
    23161 gattccactt tctaattgag taatggaaca ctcaccagct ttttctagtt ctcgataagg
    23221 caaaccgtcg cgcttttctt tgtcttgaag cccatatttg gtggctacaa agctatgtga
    23281 atcacaataa agttcgccgt tatctagcgg tacgatccat gaaacgctag ttaaatcatc
    23341 tgttttagat aaatccactc cgatataaac gggcttgcct gtaatatctt gtggttctac
    23401 ttcggttgcc tgccagtctt cagcaatcat atagctgtcc tcactcgctt gtctccataa
    23461 attgaagttt ttcaccaata cagaattaag gttattttgt ttcaaagcaa ggttcacatc
    23521 atcttgaatg gttggaatca ttactttttt gatttcttca ctttcaaaaa tcggattggc
    23581 tttgatccat ttctcttgat cgtgaatttc ttcgggatcg tccaattccc atatcgctat
    23641 aaagtacctg tcggcttgtt cttcgccttt tagcacacga tctaacaata agtattcctc
    23701 gtacatagga acgtttaaat caagcccagc ggtactaata acacacaata agccgttctt
    23761 ttgttgggtc attcctgact taatgacgtt gtacgtttta cgagtttttg cttcgtgcca
    23821 ctcgtcaaga attgcggttg ttggtgcaaa cccatctagc gtgctggttt cactagccag
    23881 tgccatagcg aacgaattag agggcaagtg ctgaatctta gagttcataa ttttcagttg
    23941 tggtcttaaa aacttactag acttgaccac gtttctaagt gaattagaca gcatatcata
    24001 gcccaattta gcttgtttca aagcgttact tacaaacaat acttgccttg cttctgctgg
    24061 ttcttgttcc ataatcaacg cattggcagc catgccactt gcgatatacg ttttcccatt
    24121 cttacggctc atactggtaa acgctcgatt gaatcgcctg taattccctt ctttagtacg
    24181 ccagccataa agactgccta gaatgaattt ttgaaatcct aacatcttga ttgctttacc
    24241 gtccgtagat ggtagcaact caatgaattt aatcgctttg gtggcttgtt cttcatcgaa
    24301 cacataaggg aagctatctg acttagaacg ctccaaatcg cttatatgac gctcacacgc
    24361 ttggataatt ttcttgggtg caatcagctt acctgatagg actttctcaa tgtaatcatt
    24421 catgaattca tcaactcttt gaacgggtct tcgggttcat catttttaac cttgttcaaa
    24481 gctagttttg atcgtgattc taaggtcata cctagtgagg tggcttgttt tttcaaatca
    24541 ctcatagctt gtgattgaac ccgaaccgct ggattggctt tctttactcc gttttcgcat
    24601 gtgataaacg tacctgattt tcttatctcg gcttcggctg ttttcattcg agcaaaggct
    24661 aaacaatagg ctaccaatga accataatct gcttcactca atggataatc tttttttaat
    24721 gttggtacga tacgattcca ttcacttcta gcgctcgctg ccatccagtc agggggcgtg
    24781 atatttatta atggttgctg cttgaacaat tcttctctag cgtccacacg gattgctttt
    24841 tcttcacttg atatattgcc ttttgtatcg tgtaatattt tagggtgtcc catgttgtcc
    24901 tcctttcaga caaaagaaaa aggaacgtaa gcaattaagc tcacattcct tcgtttttcg
    24961 atcagaattt ttctttatct aatttatcta tataaatgat ttttccgttc tgcatttgaa
    25021 tggtcacttg accaaaatga ggtaactcaa tggtgcgctg tttccctgtt ttatcagtaa
    25081 taacaattgt atttttcaat aaatctccta ctttcattat atactttttc acttgtgatt
    25141 acaagtgtaa tcgttatttg taattaccct tatgcctttt cacaacggtt atttgtacca
    25201 aaatgagtag gcatcgattg tcagtaagtt tcacactatg cccccttaac tcaaagggta
    25261 ctcttttctc gtctttgtct ttcattggct gtcttggaat tatggtgtgc atggcataaa
    25321 gaacgaaaat tagtttcatc taaccgtcta ttccaatcat cttttagttc aattttgtga
    25381 tctacaacat cagctttcct tatcaagcct tcttctaggc aatcctcaca tactggatga
    25441 tttaaccgat acaagtatga agcctttcgc catgttctac tttgatagaa ttgaaaatat
    25501 ttaccttcgc tttgctttct ttcttcacgc ttaattgccg tttgctttgc tttatgcttc
    25561 ccacaatact tctgcctata atcaatcaat gttttacatc cagcatgatt acattgtttt
    25621 ttaactgcca tagattacca accaaagcga tctcgttcgt cagaatcatc ttgttcccat
    25681 tttgatttgt attcaatata aggcgaaact atcttcaagc acgttccgtc tttaaaatta
    25741 actttagtga ttttatcttc ctcaaaacta ataaagtcta catcttcttg agtgtttgaa
    25801 tactcaatgt gtttctcacc atcgaagtat ctaatacttt ccatatgcat aattattcta
    25861 cctccaataa attagttatc aattcatcat ctgttagtag caaagccaag aacttatcat
    25921 ggatgtttaa aaacaaatta tcctcattcg atgtaagctc tcgttgtctc gctcgatcat
    25981 ataggttaag cagacaatta aattcatcca ttagctcacc tgtatcatca tagttgccac
    26041 tatctaccaa tgatcccact actagcgatt gacaccacgc tactgactcc tgcagccaat
    26101 caggttcttc ttccattatc tttaatactt ctggatcaaa tgtggaataa tcttctgtta
    26161 ccaatcccac actaaatgcc agcgtgttca ttagaaaccg atccatttca tctgtatatt
    26221 gaaagttcgg tgctttgttt gttgaaataa tattgagcat ttactttcct ccttgattcg
    26281 taacacgtgg taacacctac gtaacaggta cccgttacca ctcaatccca acggggacaa
    26341 gggttctagc gttttggtaa caggtaacgg gtaaaatcca gtaacattat tccccagcat
    26401 attagctaac tatttcaaaa gttttaagga aataaggtgt taccccgtta ccaaattgct
    26461 gtatcccttg gggcagtaag ggtaaactag taacgggcta ggtgttactg acccgttact
    26521 acccgttact ttttgtactt tccagctcga tttttatttt cataggcatt tgacttattg
    26581 taaaatcctc gtcgtttatt tctttttggc agttgcttcc gttcccaacc aggaatactg
    26641 ctaataatca aggcaatctt tttagtttct gcatctgatc gaccattcaa taaatccttt
    26701 gcttgtttat caaaaacagc ttccaaaata tcagcggtga gtacgctatc aactaaataa
    26761 aactcttgtt gcatagtagc tccattatag aaatgctgta aatttttctc attacattta
    26821 ctgtcaggat agttctgata ataagaacgt ctcgcccacg ctggagcttc ttcccattta
    26881 tacgggattt ccatttccaa atatttagtg attgcttctt taattggatt ctccatcttg
    26941 gcatcctctt gatagtcttt tgcaatttct aagacttctt tatcttcatc gggactaaaa
    27001 tagattcgtt gtcctttatc aaaaagcacc tttgcttcag ctaacacttg aagaaaatat
    27061 tcatcctttg ttttaaacgg attcatcttt ggcttattct tacaaggcaa aggataaaaa
    27121 cggcggtttc ctgtatcatc tttcaaatac tgcccatcat tcgacgttcc aataaataca
    27181 cattgccttg cccaagctat gacattccga ccataaggag gtctaatttt atcgatcttg
    27241 gcactgatga agttttttat ctgttcaatt ttagttttgt tcattgaaga taattctcca
    27301 agttcaataa ttacattgcc ttgaagctgc atataatcat ctttcttttc acctagcgaa
    27361 tctaatgaat cggtgaaaaa atcatccgtg taaagtaatg ctggaacggt actcttacca
    27421 atcccttgcg gtccatccaa aactggaacg atctcaaact ttattcctgg tttatagata
    27481 cgtgcgactg atccgactat ccaacgtttt gttacttctc gtgtgtaaag gttgtcgtct
    27541 acccctaaaa ggtcaataaa cagtgtttcc agtctaggct gttgatccca tttaactttt
    27601 tcgatacgtt cttttactgg gttgaaaaca ttctttctag ccacatttac tactgctttg
    27661 tgtattttgt tgtcatcaaa aactacattg tagttttctt caatatatgc cgttaaggca
    27721 tcctcaaaac tgtcttccca ttctccagcg gtacgattga tccaaggaga atcttttaat
    27781 aggtgaatgt atccgctaaa ttcgttataa gcaattgttc cagctaaatt gctatcattg
    27841 gttaaaatca attctgcatt cacaacactg ttacttttaa tctgatcgtc tttattgcat
    27901 cttaatcgtg acatccaggt tttcaattca atgatattgt cttcacgctc gctggcttcg
    27961 ggaaagtcta aaatactatc taatttatgc ttatgagcca tgagctttcc ccctcaaatc
    28021 ttttttaatc atcgatcgaa taatttgatt cacttcttta tcttttagcg gtggttcatt
    28081 gtacgcattt gctgtataaa tcaattctgc taaagtttga ttatctacac cgtgccatag
    28141 taaccaaccg cataacttag ttaaatgatc gtttctacta ccttcgccag cgccttgaac
    28201 aatcatatct aatttttttg ctgtccctgt ttttgctacg ggtcggtatt gagtctttat
    28261 ttcttgcggt ttttttagca gtaggttcac tagccaatcg ggagctggtg caatattatt
    28321 tagagatacg ttttcaatag gttcatatgc tctcccaaca cttcttgaaa taatcatagt
    28381 tggcgctatt ggaactcctt tagtgataat atcaatcccg cttaaacttt tacctttatc
    28441 atcacgcccg aagatatcag caaatgcacc tgtttcttgt tggataatta caccatcagg
    28501 aattttaaag aagaaatgga ttcctttacc tggtgtgatt tccgtataag tagaaggaaa
    28561 tggatcatac attgaagcaa gttttttata attctcaacg ccgtttcgtg tttcagaatg
    28621 ttgatctagg tcaactacca ccatttgatg atcttcaagc attaacccaa tatttctagt
    28681 tgcatcttcc gtccaccatt cattaatttg attagaatcg ttagttgcat cattccaacc
    28741 actagagcct tttattggaa ttttagttcc aggagataag ggataaattg accatcccga
    28801 cttttgtaat tccgttgctg cttgataatt atttgccaat aaacctctcc tcctttttca
    28861 aaatactggt ataaatcatt cagacacctc catgagcttc aactgctcaa gttcgtattt
    28921 caacccatcc cgaacaaatt cagcaccttt taattgattc tgatacttta cccatgtatt
    28981 aaatgttttc acccttgctt cttgcatatc agtaatgttt aaccatgcgg ttaaaatctg
    29041 attttttagc tcgttgattt ctcgacaaat tattttgtgt tctgcatgtt ttttgagtac
    29101 atacaaaact gttagttttt cattgaatga atttccaaag taggttttcc cgatattatt
    29161 ttctagttca gcgatccctt gattaatttc ctctaattca aggctatcga gttggtttaa
    29221 tagttttgtt ttgtacatct acttatgccc tccagttctt aattgatagt cgtactcgtc
    29281 ataagcagtg aagagatata acgctaatcc tgcaaagata gctatttttg caattaatgg
    29341 taataagccg aaaattccgc cgattaaaat gcttcctata attaagtttc gtttcataat
    29401 ggttccctcc gattttttaa tatggtataa tcggaacaaa gaatatcggg ttttatccca
    29461 attaagttgc ctttatcctg tccgccaaga ctgttatgga taaggctttt tctttgttct
    29521 cgatcatgtt aatgctccta agttgtctgc aattcagcag aacgattata aattttctca
    29581 ctcactgaaa actgcaaccc atatttttct ttaacagtca ttaattctac tgtctcatct
    29641 aaaataggtt cacgatcaac caacatttca ggagtcatat ctttcttttt taccattttg
    29701 gaatgaccat aaatagttga tacagcttta ttggctatca cattcgcttt aatgtagtct
    29761 actggttttg gttcagtaat agcgtggctt aatttagtca ttgcttcttt ttggtgttct
    29821 ttgtctagca tgcggaatac ttgaaagcct tcaagtcctg tggactggcg tagttctttg
    29881 atgatgtcga aaatccagtc ttgaaatgcc tcggcttctt ttttacggct tttgaatgcc
    29941 atgcggtaaa tatttttttt cgttgatgat gtttacatct tgtgttctgc ccaatgaatc
    30001 gatgacctta ctagtagtaa ccccatcttt aggcaaagag tgaatggctc tagtaacctg
    30061 tttaagacct aacgcttccg caacatcttt cgccactgcc caccattcat cattaatatc
    30121 gacaaatctt ataatgtgtc cgttccaaat ttcagttttc attcaacgtc ctccagttca
    30181 agccctagca agctcgctaa caactcaact tgctgataca aaatttcttg atactcttca
    30241 atagaaagtc gttctttaaa ctctgcttgt actaaatcgt tcaagccacg caatactccg
    30301 cttaccgttg gttcttgtgg tagcaatttt tgatagtcca tttgttcgta caaacattca
    30361 aacgtttctt ctagcgcttt gccttccaag tttgctaatt ctgccgctgt gtcttctgta
    30421 agaattgttg ataatacttt ttcgattgct ttattttgtg tcgattccat tattatttcc
    30481 tcctattaat ccctttgccc ccacggcttg cgtttatttt tggtattctt ccataaagcg
    30541 atcacattca tcttgtgaaa ttcttacgac tccatcaact ataatcaact ttaaaccgtt
    30601 aggaacatat ttctgcatca tagtattgta tgaaacgttc atatacttag cagcttgtgc
    30661 cttgttcata tattttggaa actggtactt tttttctaat aatgaaagta acgttccttg
    30721 agtttgctct acttgttgaa caactcgatc gtcaataatt ttaaaagctg gatcatcaag
    30781 cgtgagtttg tcaattttta attccatatt tatcccccct cgttagcata catttctata
    30841 caaagatttt aaaaaaatgc aagaggctct tgaatacatt tctatacctc ttataaactc
    30901 aatataccat catacctttt catttgcaag aattttaaca tacttttttg aacttttatg
    30961 tttcacacat atttaaatcg tgatatagta gagaaaatat atgaatggtg gtgaaaatgt
    31021 ggctagaaaa aaataccccg aagcagtgga gacaggacaa agaattagac tggctagaat
    31081 tgaaaaggga ctctcaatgg aacaacttgg aggtaaatta tccccaccgg ccagtaaagg
    31141 tgccgttagt aattgggaaa atggctataa tcttcccaat aacaccaggc ttaaacaact
    31201 ttcaaaggta ttagatgtat ccactaccta tctattaaac ggatcttata cattaagaga
    31261 tatgcatatg atgcccgaaa ttaagcgaaa tgtgcttgaa aagcaattga aaaaaactat
    31321 tgaaaaaaat aactctataa ttgattctaa tctttctaat gctatcaata acatcgaaga
    31381 agataacctg actgtctcac agaagattta tttaacaaac gcaataaaat atcttaaaac
    31441 aggatctcga attgaatttc ctgacgggag taccccaatt gcttatctcg cagctcttct
    31501 aaatacgctt ttacgatatg actcattaat taatgattca gaaaaaaata cagatgagaa
    31561 aaaagagtat caagaatttt atgaaaatga tcttctaaaa gagttcacag ctcttgttac
    31621 tgagctaaat aaacactatt caaaataata ataaactgcc tcctccaacc gcccccacgg
    31681 cttgctacac tatggaggga aaactattat ggcaacattc aagcaatatg aaactaaaaa
    31741 aggtaaattt tggctctatg aagcctatct tggaatgaac aaaatgactg gcaaacctga
    31801 taaggttcgt agacgaggtt acaaaacaaa aaaagaagcc cagcttgcct tgtcacgctt
    31861 gcaagtggac tacgataaaa acggtattaa aaaagcgaac aacgaaacgt ttaaagaagt
    31921 ttatgacttg tggctagaaa catataaaac cactgtccga gaagccacct tcatgaaaac
    31981 tgaaatcaag tttagaaagt ggattcttcc aaaatatgga aatcttcgtg tgaatgaagt
    32041 cacggtaaaa aaagcccaac aaattgtaaa tagctgggct gaaactactg accaatacaa
    32101 ggtacttcat tcaacttcta agcgtatttt tagctacgct atcaatcttg ggattattga
    32161 gactaatcca ctagacaacg tattaatgcc taaaagaacc accactaagc ataaagaaaa
    32221 ggtcaaagtt tacagtaaag aacaactgat caccttgttt gattacctaa acagcaaacc
    32281 tgaaacttat cgaaacgact ttgataaaac gttactacgc tttctctttt atagcggggt
    32341 taggatcagt gaagcccttg cattgaattg gtcagacatt gattttcaag aaaatacggt
    32401 caccatcaat aaaacactaa gccaatccaa aaatggctat aaaatcagtg atcctaaaac
    32461 tgattgtagt gctggggttc ttccactaga tgaaagaaca atttctatat taaaaaaatg
    32521 gcagattaat cagcggaaat atatgctgac acttggtgta actgatccga ctatgatttt
    32581 ttgtggtatc tataaagaac taatcacaca ccacgctatt tatgcaagat tgattactat
    32641 ttgtgaaaaa gctatggttc catttctcgg tgtgcatgta gcaagacaca ctcatgcttc
    32701 aatgctcttg gacgctggag ctactatggt agaagttcaa actagattac ggcatacaaa
    32761 aattagtcag acgatcgatt gttacggtca cttggcaaaa gaaacgaaag aaaagactgt
    32821 tgaaaaatta gtacaacact taaatattga ctagatggca tacaatatgg catacagtaa
    32881 aaaataaaaa aaggagaaac cctatcatat caaggtttct cctagcttta tagtctattc
    32941 ttcttcttcc gccatgaatg tattgaatac ttcttcaatc atgtcccatt ctgcgtctgt
    33001 ttcgatttgt tctaactcgc cttctgttcc atcttcgttt tcgatgtatg cataagcttg
    33061 taattctaca tcttcatctt ctgaaacacc agcaggatat aaaagaacat agttacgtcc
    33121 aaattcttct tgtccgtcaa ctgttagtag gatttcatat aatgtttcat ttccttggtc
    33181 atccaccaat gtgatgtgtt cgtgtccttc atggtcgtgg gaatggttat gttctgtcat
    33241 aagattacct cttttctttt attattaaaa taattgctta cctgagccgt ctaaataatt
    33301 ttgtaagatc atcacagctg ctaatttatc gatgactttt tttcgtttgg ctcttgaagt
    33361 atttgcctgc tctactagca tacgttctgc ctgaacagtg gttaatcgtt catcttgata
    33421 atcaactggc aaggagaata gttctttgat cttttctccg tatgacatgg aagcttctgc
    33481 ccttggtccg atggaattgt tcatattttt aggtaatcca actacaaacc ggtctacttc
    33541 atattgtgct actaattcct tcaaccgatc aaagccaaac tcttcttctg cttcattgat
    33601 ccgaatgatc tctactcctt gagctgtcca gccaaagggg tcactgatag caacacctac
    33661 tgtcttagaa ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg
    33721 tagtccttga caattacttc catgatctca tcgcgttcgt gacggcgaat caaatttcga
    33781 gcatcacgat aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga
    33841 ttgattggat tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc
    33901 acttcttttt tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg
    33961 attctacacc tctttcccgc aaggtaattc cagcttacct tctacaacct tattttactc
    34021 gaagaaaggg aaaactacaa acaaatattc caaacttccg aataaattaa gaaacactat
    34081 ttgtatgttt ttttcctttt ttctgtagct ttatcaaaag tactcttttc ataaagtata
    34141 cgctatattt ctttgaatgt atgtttttac acaatttttt cataatattc ataaaaaatt
    34201 cgaatagctg ctactaattt tttgtttttg ttgcttgtcc tgtctcataa ttgatcgtgt
    34261 agccatcttg aacattgtag ataaagacat tgaattcaat ttttttgtct tcaatgcttt
    34321 ctgcttccaa ttgcactccc ctggctacca gttcattccc ctcaaaatga ggagtgacac
    34381 ggtagcgtac atgatgattt gtttctttga tatatgccgc aatatcattt tcgtgagcaa
    34441 gcatgtatgg agcattcaac gaacgtgtcc ctgtcatcaa gtttttaatg ttgttatttt
    34501 gaccagtcag ctggaaacca attaaatggc tgcgattata taaccattgc ccgtcactta
    34561 atttcttttg tttccaacct gtcgggtcaa tatacaaagg ctctctcttt tcagtaggga
    34621 acaaatcttt tccaagcata gcattggctt gtcctactct gttcaattta tcaatatttg
    34681 aatagctttc ccatgttcct ttggaaaggc taagatcctc ttttgtaaat tccggctgat
    34741 tcccattgat ttctatttct tgatgttttc catcatattc tggcacatcc gctacttgag
    34801 aagcttgtgg tgtggaatca aaaatagttg taatctgctg aaagatatct gaatcttttt
    34861 gtgtggtgga aacaccgaat gcccctgcaa tcacagcagc gacgatcccc acgatcgcct
    34921 tggttgtttt tttgctcatt tctttactcc tttactaaca gctccctaaa tcttaacata
    34981 tgttccccta taaataaata ctagaaagaa atagaaacag caaaaaataa aacctgtttt
    35041 aagatggctt aaaaatacaa aaaagagacg gattcctcgt ctctaatcag tcaccactct
    35101 atctgtaagg tatttattat tattcaaaaa ttttcttcat actcaagaca atacactgat
    35161 ttcacaaccg cttggtaaac ggtgcgcaaa agcttgcatc tgcggtaata agtccatcca
    35221 agttaaaaac ataagaagtt gtttttgctt ggctgttctt attacttgtt gcagagcagc
    35281 tttttcacaa cctattttaa gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa
    35341 gctgggattt taccactaaa ctacacccgc aatacaaaaa cagtatacgc ctcttttatt
    35401 ccttcctcaa gtttttttat tctttacgct tctggtatgt tttgtagtaa aataaaacta
    35461 atcaatcaat tattggagaa gatgctatga cgggaaaaga attagatctg ttgctggaca
    35521 cttgtttact cgctgggaaa atcatgatgg aaagcaatgc ggagatgtat cgggtagaag
    35581 atacgatgag tcgaattgct ttagcttctg gaaatttccg tttggtcagc tatgtcactc
    35641 aaaccggtct ctttattggt cttgatcgta cctcgaccat ccgaatggaa caaattacca
    35701 accgttcgat taatttagaa aaagtagtaa aagtaaatga tctttcacgt aaatatgttg
    35761 cttcagagct aacattagaa gaactttact gcgaactgca agcaatcgac cgagaccgtt
    35821 ctttctttcc tgtctggttg caaatcgtta gcgcggcagt gatcagtgga acaattatgg
    35881 tgttatttgg cggaaagatg accgatttac ctgtgacttt agtcattgga ggattgggat
    35941 acgcagctta tctatatagt ttgaaatttt ttagaatcaa atttttatcg gaatttttat
    36001 cttctttatt gattggcata gccgctatct acagcgtacg ttttggctta ggaagcaatc
    36061 aggatctaat cattatcgga tgtgtgatgc ctttagttcc tggagttcaa attacgaacg
    36121 ctatccgtga tttgctagct ggacattatg tctcaggagt ttctcgcgga acagaagcga
    36181 tgatgactgc tacgatgatc ggttttgcga ttgcttttat tttccagctt ttttattagg
    36241 aaaggaacac aagtatgggg acgatattta ttcagttttc ttttagtttt ttagcgactg
    36301 cggcatttgc cgttattaca aatgtaccta ggcgttcatt gatttgttgt ggtctttctg
    36361 gtgcttttgg ctggatgatc tactggatct gtgttcaaat aggcggatct gctgcttttg
    36421 gttcattgct tggttcttta ggagtagcat tcatcagcga tcttttctct aaacgtttga
    36481 aaatgcccgt aacgattttc aatattcccg ggatggttcc tctagtccct ggtggtttag
    36541 cttatcaagc ggttcgaaat ctcgttactg aatcctatcg agaagcggct cattatgcgg
    36601 tccaagcaat tatgattgct ggagcaatcg ctttaggtct tgtattatca gaagtcttta
    36661 accataacat ccgtaatttc aaagtaaagc gtgacgcaat catgatgaaa cgaaaaaaac
    36721 ggctttttaa ataaacgaag tgtaacaaag aaaaggttgt gaaagagacg ttcagccttg
    36781 agcgatgaca aaatatctca caaacattgc gacaagtaac cgcaggagca ataagaagta
    36841 attatgagta ttaaaaaaag ttttgtaaaa atagcttctc acatttttac ataaaattga
    36901 cttaatactg agaaactgac ttttgaacac cctctatctg taaaaaaggg gctgcgacat
    36961 gatttttgtc acggcccctc ttctttattc aaattttttc caaaacgttc gttgataata
    37021 aacgatccat agagcggaaa gtattagaac taaaccgatc acaggaatat tcactaacca
    37081 aaatggataa aacaaagcca acattccgta taagacgata agaataatac tgatgatcaa
    37141 tgaacccatc atagttgcga ctaaacccaa acttcctgaa cctctgttaa ataattggtt
    37201 aatattggtc cagttcagca gcagcaaccg ataatctcta gcgaaataac gtagtgacaa
    37261 taagtagcag ccgaataaag aaccaaatag caagctaggg ataaaaaaca aaggcagctg
    37321 aaaagacagt ccgcttaata atgcgatccc accagttaaa aaactctgca atatccatcc
    37381 gaatctgaac ttttctttta aatattggtt catagagatt ggcaaagaac ggataaacag
    37441 aaaattttct tgatccaaag aaataagatt gctgataaaa gaagtctgat taactgttaa
    37501 gaaagccaat gcgatcccta ccaggaaaac aacaccgatt aacttattat ctaaatcagt
    37561 taattttatt tcgcctccta gggcaaaagc aagaataaaa atgattggag ttaacaagga
    37621 agaagaaaga acttgcataa tcaaattagg ttcttttact aattgagcat tgtaactaaa
    37681 taaaagctga tgcacatttt ggttcttttt atgggttctt tttattttga actcactgtt
    37741 tgaatcagtc agttgctctg taagctttgg tactatcagc tgttttacag caaggaaaaa
    37801 tcccagagcc gtgatagcta gtattcccca acccaataaa ccttgttgag aaagtggctg
    37861 actggcagca tcatagaatg gcaataatat cgaaatcgga tgccgatctg ttaactcatt
    37921 ttcaatagtc cccgtttgat gattcatcca catgattcca gcaaatgcga tcagcatcga
    37981 gctgccaagc aagatactag aaactgtttt tctatgtttt ttaaacactg ctgttcgagc
    38041 caaaccaaaa acaatcaaac tagccagaca aaaaagaata atcaaaaata gagaaaacaa
    38101 aaggattgag ataatgatag taagcaaaat ccctagatca gctcgccatc cggttaaaat
    38161 aaacatgact agaagcggaa aaacaaaagg aatgatggtc aaagcaacta ccaaaatctt
    38221 tgctgaaaaa acctgttctt gtccaaaagg cagaggcaaa aatacttgca ggtcatttcc
    38281 ttcaaaaaag atattataaa tgactgatat actttgagtt aaacctaaca taccaaaaag
    38341 tgccacgtaa taagtaaaga aacctggcaa tttaacaaag ttcatagcaa acatagcaaa
    38401 tccgtaaatc agtaaaaaaa tcagccctga aaaaagattt tgagacaaaa gatagatagt
    38461 taatcgcttt cccttttttc ctttctctct tgctttattc gtgagctgtg gattaacata
    38521 taaaagatta actcgaatta attctaataa ttgtcgctta tacatacggt ccatctcctg
    38581 tcgcttgcct tccagccatt ttcaaataaa tcttttctag cgtttcatca ggtgagccat
    38641 tcaataattc actaactgcc ccctgataaa tcaattcacc tttcttcaaa atagccagtt
    38701 tatcgcataa ctgttgtgcg gtatccaata catgtgtaga aaaaatgact gtttttcctt
    38761 ttgcggcatg agccttcatc atttctttca aatcaaatgc tgcttgagga tcaagtcctt
    38821 gcaaaggttc atccaatacc caaatatctg gatctggcag caacgtgccg atcaaaatcg
    38881 ttttttgacg cattccatgt gaaaaacttg cgattgtttc attttgatga ctatacatat
    38941 caaaaagcgc tgtcaattct gctaaacgct tttctttttt ttgtgtgtct aattcatagg
    39001 cagctgaaat caagtcccaa tattctccag ccgtcaattg taaaaaaata tctggggaat
    39061 caggcacata accgattttt tgtttgatac tcaatcgatt ttccgtcaat ttcattccat
    39121 ctactaaaat cgttcccgat gatggttcaa taatactaac taaactttta atcgtagttg
    39181 attttccggc accattatgt cctaaaaatc caaaaatttc cccttgtctt atcgagagag
    39241 acaagtcttt taatgcttgt tttttcccgt aacttttact tacattgatg aattcaatca
    39301 tttctgtact ccttttgttt gttattactt tttattgtac ccttcgttat tggtggagta
    39361 aatgtcaaaa agagtgcata acaaaaaagg atagaaacga ctaggctgct acttgggttg
    39421 ttctcttcta caggaaaaca ctcttctaac aggtaagata tgagtctttg ttttctttaa
    39481 aatgattcat tttctatctt ttcttgcata aaattcttta gtttatcaat acttctcttt
    39541 cccatctcta gttgttctcc atttgcaaat gttactattt cgtttactct gctaatagat
    39601 gtaatgtttt cagtattaat gatataagct tttaaatctt tacataaata ctcatattcg
    39661 agtgattttt tatatttttt aattgtgccg tttacgagaa tctcttcatc tttcaatact
    39721 aggcctattt tatttttata acctggcatg ctcattagat aaaggatttc atcgtaatgg
    39781 atatagtgat cgctattacc ataacggaca gttgtccacc tttttttcgt agcatgttga
    39841 acaggatagt tttctttttc tttcaataag taactttgga tgcttttttt atttttttca
    39901 taagtgaatt cttttatcaa ataaccacta ggcgtaatct ttcgattaat aacagttatt
    39961 gctttattcg gatgagacgt tagataaaca attttacact tttcattctt ttctctaagc
    40021 atttcgccta gttcgatacc gctataatag gcgttcaaat cgatgtctag gaaaaaaata
    40081 tctgaggaat agatttcatt atttttaatg aattcaacaa attgtataac gttcaatccc
    40141 tgaacgatct caaatttcca aggacttaat aaaattctgg attcttccaa aatttctact
    40201 agcatttttt caaaaaccaa actatcatcc aaaataaata tacgcgttac cataaaaagt
    40261 acctccactc gatattttac tatcaaaatc atttcataca acttttcaac atacatgtta
    40321 ggaataaatt gtaattctct tcacaatctt ttccaacacc tttgaaaccg atatcaaata
    40381 ctttcaggat gctgcttcta aaataaatat caaacaaatg aatacatcat gcaaaaaaag
    40441 ccagacaacc gacgtccttg cgtgtcagct gtctgagcta tgtttattta ttttgttctt
    40501 ttaaaaagaa cgatacacat actgattttt cggagcagag actttctcag cgtttgtaac
    40561 aactacagat gggatttcac gcttgaatgg ttgataatag tctgattgga tggttccttc
    40621 tattttatac cattcatcat ttttaaccgt tactccttcc ggcatatgcg tcaacaaacc
    40681 aaagactcct gaatctgcca cacaatggat aatgccgaat cggaacaaga agatatctgt
    40741 attgctatcg ttcggtgttt ggtaaacaaa tccttcatag ctgatttttt tgccaatgaa
    40801 ttcgctaggg taattataaa ttagctccat aacttctaaa tagttttcat ctgtgatctt
    40861 gatagtttgc ttatccccat acttttcttt caactcattc atttggtcta agtagtctga
    40921 tttgttgaaa taaatactgg tatccggctt taagtattgt gtctgcatct ccggatcccc
    40981 tactgattct ttacttactg ggaaattaaa tcctttggct tctacgatcg tagtgtcgag
    41041 actaactgtt ggaaacaacg tccccacgat tactggcaaa gctagcagta cataagcgat
    41101 tccacgttga taaggttttg acaacccatg gtcatgatcg tgctcgtgtg ccttttcttc
    41161 tgcttttccg tttttgaccc acaaatacag ctgaactaaa gccaaaagag cagataaaat
    41221 cattgaaaga atagctaagt agcgataatg gacattgata tattgatcga gtttgccaga
    41281 aacttgcaag tacatcatca attcgaaata cccagataaa atcaaaaacc gcagcatatt
    41341 ttccctccta aataaataat gtgaatccaa taacgctaag ggcaatcagt ccgatcagcg
    41401 ataaaataaa gcgtcctgta aaatatcgtt tcatcatcag tagatttttg atatcgacca
    41461 ttgggccaaa gaccaaaaag gcagctacag gtgctgtgcc gaacagactt aacaaagaag
    41521 atcctataaa cgcatctgct tctgagcaaa gagacatcgt aaaggccaat aacagcatga
    41581 tgataatggc taaaatcttt gttgagccga gcgttagcat aattctagtt ggtaaataag
    41641 tctgcatcgc agctgcgatt aatccgccaa tgatcaaata acgacctgta tcaaaaaatt
    41701 cgtcgattga atgtgtcaac acatgcccag tttgatggat cagctgattt tttggttcat
    41761 tcttctcact ttgttcttct tcatggacat gatgatggtg attttctgct actttagacg
    41821 tgattttttc tttcaataca gcgtcttttt gaaaataggc aagccagaca ccaatcacaa
    41881 gggcaacaaa aaagctgcct atcacccgcc agcttgccat ctgccaagaa ttgccaaatg
    41941 cgataaaagt ggaaaaaatc acgattggat tgatgatcgg agcagtcagc ataaaagcaa
    42001 acgctgtgtg aactggtacg ccttttttaa caaattgatg aacgatcggt acgatgccgc
    42061 attcacatga aggaaagaaa aatcctaaga tcgaaccagt aaaaatagag aagatacgat
    42121 tttcaggaag ccatctcttc acacgttctg gggtcaaaaa tacttgtaag gcgcctgaga
    42181 taagacagcc taataatacg aaaggtaatg cttcaataac gatcgataag aaaatcgtac
    42241 tcatctgcaa aaccgaattt ggaataaaag aaaacatgag tttcctcctt tttatttcta
    42301 gttcattcgc tttgcaaata tacgctttta ttcaaaaaaa acaagttgct ctgtaaattt
    42361 ttacacaact cttatttttt accaaagcac ttcaattgac tttttctgtt attcccatta
    42421 aattaaggtc tcatacatat cacttctatc ataaaaggga gaataaaaat gaaacaaacc
    42481 actaacttga ctgaagttca agacatactg caacagtcat ccgttgccgt catttatctt
    42541 tcgatgccta attgcagtgt ttgtcacgct gttcgtccac gactagagga acttctcaca
    42601 cattatgata ttccggcact tcatttggat gcatttgaaa caccagaagt agctagtaaa
    42661 tttgaagtgc tgactgcgcc agttgtcctg atttttcatc aaggaaaaga agtctttcgg
    42721 caagcacggt tcatcgactt taaaaaaatc cgttatctac tagatgaact gacagcagaa
    42781 gatgattccc tttcttatga agaaatcttc cgaacaaaat aatcgttgtc caaacagaaa
    42841 aaagcgattg aacaagtgaa agttttgcct tcatttgttc aatcgctttt tatttttatt
    42901 ctgctgtatt ttcggaatgc gcatttgcgt cagaatgcaa gaaatagatc aaggtttgca
    42961 attcattcgt caaatcaaca ttttggatca atacatctga cggagcattc aatcgagcag
    43021 cggtaaagtt caagattcct ttgattcctg cttccgttaa ctgattgact acttcttgtg
    43081 ctctttcagc gggtatcgtt aaaattgcta cttcgatttg ttggatcttg atttgttctg
    43141 acatatcttc cattggataa acggggatac cgtctacgat gcgcccaacg atttcctcat
    43201 tcacatcaaa agcacaactg atacgtatgc tattactttg atggaatttg tattttagta
    43261 aagcacttcc caagttccct acaccgatca gcgcaacatt tgtcaattga tcgtcgttta
    43321 atgtctttgc aaaaaagtgc ataagatctt ctacatcata accataccca cgttttccta
    43381 attcgccaaa ataagaaaaa tctcgacgga tcgttgcact atctacttga acggcttcac
    43441 ttaattcagt tgaagacact tttttctttc cagcattatt taaaatcctt aaatagcgat
    43501 aatacaaagg cagtctttta gctgtcgcct ttggaataat atgatctttc acgaaatgac
    43561 ctccacgtat tgtctaatta catctttgat ttctccatca tagcagtatt tataggaaaa
    43621 aatcaattcc attgctcaga tattgtgaaa ttttattctt tattcattta tttatcattt
    43681 ctgcaaatct cgcagtataa tggaatttat ggtagactac taattgaaaa atagtttttt
    43741 atttgaggag aattcacaaa gtagaaaaga ggattccatg atattattac aagccaatca
    43801 gattgctcgc cattttggtg cagaagtctt atttgaaaac atacatttag aaatcagtac
    43861 aggcgctcgt gtcgctttag ttggcagaaa tggcgctgga aaatcgactt tattaaagat
    43921 cattgctgga attgaagcac ctgatgaagg aaagatcgct aaaaataaaa cagcgactct
    43981 cggatacctc gctcaggaca caggacttga ttccaatgaa acagtttgga acgagatgct
    44041 gaaagctttt gaacaagtta gacaaatgga agagcggatg cgcgagctag agattttcat
    44101 tagcgaaggt acgccagaca cacctgctta tagttcttat ttaaaagaat acgaccggct
    44161 gcaacacgat ttttcagatc gaaacggcta tggttatgaa aatgaaattc gttccgtatt
    44221 gcatgggttt aaattcgatt cttcatttta tgatcagaaa atcgatactt tatcaggcgg
    44281 acagcgcaca cgtttagctt tagctcgcat gcttttgcaa caccctgaca ttttgattct
    44341 agatgaacca acgaaccatt tagatatcga gacactttcc tggcttgaga attatctgca
    44401 aagctatact ggtgcattat tgattgtttc ccatgaccgt tattttttag ataaagtagt
    44461 caatgaagtc tatgaattaa gcagaagaaa aatgcgccat tacaaaggta actattcgaa
    44521 atatctagat ttgaaagcag aacagttagc aagtgaatgg aaggcttatg aaaagcagca
    44581 aacagaaatc cataaattag aagacttcgt tgctcggaac cttgttcgcg cctctaccac
    44641 aaaacgagcg caaagccgca gaaagacatt agaaaaaatg gaccggctgg atcgtcctca
    44701 aggggatgaa aaacaagcaa actttctatt cagtatagaa aagccttctg gaaatgtagt
    44761 cttgcaaatc gaagatgccg ctatcggata tgataatgtc accttatctg aaccaatcaa
    44821 tctagatatc cgtcgtcaag aagcgattgc tctagtaggt ccgaatggta tcgggaaatc
    44881 gacgttgctg aaatcaatca ttcaccagat cccttttatc cgtggaaaag aaacgctagg
    44941 gacaaatgtc accatcggct attacgatca aggtcaagct gatctgcata caaacaagac
    45001 gatcctagca gaattgtggg atgagcatcc taccactcca gaaaaagaga tccgaagtgc
    45061 tttaggtgct ttcttatttt ctggcgaaga tgtagaaaaa ccaatccctc tgctaagtgg
    45121 cggtgaaaaa gcacgggttg ctttagccaa gttagcgatg aacaaagaaa attttttgat
    45181 tttggatgaa ccaacaaacc atttagatat tgacaacaaa gaagtattag aaaatgcatt
    45241 gatcgattat gacggcacac tgctttttgt ttcccatgac cgatatttca tcaatcggat
    45301 cgcgacaaaa gtcgtcgaac tctctgaaaa tggcagcaaa gtctatctag gagactatga
    45361 ttattattta gagaaaaagc aggaagaaga agaaatcgcc gaattgttgg cacaagatga
    45421 gccagaatct gctcctgctc ccacgaacaa atctgattat taccaaagca aagaacagca
    45481 aaagcttatc cgtagccttc aacgaaaaat tactcaggtt gaagaagaaa tggcgcggat
    45541 cgatgaatgg atcgatcagt tggaacaaga aatggtcgcg ccagaaaacc tggaagatca
    45601 cgtaaaactc aatgagctga accaagattt agaagctgct agacaagatc aagaaacaaa
    45661 attgacggaa tgggaagaac taagcttgga attagaagaa tatgaaaatt aacgaaaaaa
    45721 ccaaaacaga aaccattcat gctgttttgg tttttctttt tttattttat caaacaaaaa
    45781 ttccatattt acctttttca ccaataagaa gaccaattac tagtcattct cactaaagct
    45841 tctaaataat agtaatcacc ccaaatacaa ggtgtattta ccccattatt tttgggctta
    45901 ttataaacag actgtgttaa aaagccatct tcttcttccg ttgtagcata tctttctttc
    45961 aattgtcgca taattttcca aacagcagac tggtataagt tcttttctgt tttgtctaat
    46021 agacctgatt ctattaattc taataatcca caaaccatga tcaccatact agatgtatct
    46081 ttttcttctc tgctagaatc atcaaagatc aaatcccaat aacaaatggt atcttctggc
    46141 aaccgattca taaaataatt ggctaatatt ttgcttaaat caaaatattt ttgttctttc
    46201 aaataacgat aacttaatgc aaaaccgtaa atcccccatg cttggcctct agcccaacag
    46261 gaattatcac tataaccttg atgcgtactt ccatatcttg gttttccatc tttgacatcc
    46321 atataaaatg tatgataagt tgaaccatct tctcttacta aataatctgc tgcattttga
    46381 atatgagcct gagcagcttt tttatatttt tgatttccag tttcttcact cgcccaatac
    46441 aataatggaa gattcatgca acaatccatg atcattctac cttgttgtga aggatcacta
    46501 ggatctcccc aagcttgtat aactcctgcc ttctcccaaa aacgctgaag taatgtttct
    46561 gcagcatcaa ttgaagcttc cttcattttt cgatttcccg ttattttata atctgctacg
    46621 gttgataaag aatacaaaaa accaagatcg tgatgattag tgataatttg acagtcaatc
    46681 ctgtggataa aatctatatc tgctttttca gctgtttgtt gaaataaagg atcacccgtc
    46741 ttctcaaata acaaccaatt aattcctgtc caaaatcctt ctgtccaacc aatattgtct
    46801 cttaatggat aacgtccttt tataggacac tcgcttggga attgatactg actgttactg
    46861 atacgtttga ttaacctctc cgtgtgtctg cgacaacgct cgatctcatt attccattct
    46921 ttttccatta attctttata acattcattt atccattttt ctggatgtat tatctcttca
    46981 aacatcactt tatcaagcat ttccccacca gcctttagca taattttagt ctttttttat
    47041 tgtttttatt ttggatagtt agcccggttg tcccatcaaa atcaattttg ggacgattgt
    47101 tccatacttc taaactataa tcccgatctt gatgagcaac cactcctgtt ccgagccaat
    47161 gggttccttt tccaaatgta ccctctaatc caggaataaa agtgttttcc catgtcatca
    47221 agtttgtatt gggcaaaccc ataatagctg tggattttcg tttaatagta tcgtcactta
    47281 aacagataat acctgaaaag ccagcttcac tcaccctata gttttccttg tcttcttcta
    47341 tgattattcc ttctttctca ggagaacatt caatagcaaa ccctgtctca tatagaacat
    47401 attctgtatc taattctact ttgtgtacgc gaatctgcca gccttcaaaa ggaataagcc
    47461 aagttctgat ttttgcctgt aaagtaggtt cccatgtaga ggcaagtacc tctttcttac
    47521 atacaatgtc tcgtacatta tttcgaacga tgaactgtcc ataggttttg atgctctcat
    47581 tcttaaaact ggtcgtgata tatgtgtccg ctcgctgaat ccctagcatt gaatccattg
    47641 ccatttctgc tttcaattga ttcgctcgcg gtatcgaaaa accaaattga ctagaataag
    47701 caaattttaa atatttttca ttggtcaatg tatggaaatt tggccctaat tgtcccgcat
    47761 tcaaaaaaaa tgtatgtcca ttatcatgtt gtatgagcaa attggcttct gaaagaagtt
    47821 tagttccttc acgaataggc ataggcattt ctttagccgt ccaatactca tggtcttcag
    47881 gtagcgccaa caataaaaaa atcttattca gccataatgg agataacgta ctattatagg
    47941 gttcagtcaa tgtcaattga gggtaggtat aaccaatatt caggatacct ttttcatcaa
    48001 atatttcttt ttgcatccac catcttatat tccgattgac taaccccttg atttcaccta
    48061 atttaaaagg caaaagatcg gcccacaaca tagctgacca aaagcttgaa actgcaaaac
    48121 gataaatcat gcttcgacca aaaggcacat ttgcaccatc ttccgcaaaa aaatgaatat
    48181 agtctctaga aaaatcaata gctcggttct tatatatctg gctaatgggc ttttctggtt
    48241 ctagtacgct ataaatcaaa ccataataat gaaatgcaaa aggattataa taatcttggc
    48301 gacctcttga actgtctcga taccagccat tttgaagata acaattttct atattatcaa
    48361 atgcttcttt cagtgctgat tctttaactg gataaccaaa tttttggata attgttccga
    48421 caattatctt aaaaaattgc caattaccat ctgcacattc atattcaaaa atttgcgaga
    48481 accaatgaat aatctggctt ttttctcctg tatctaattt tttccataat aattgttcat
    48541 gatgatataa cgctaataac attgcgggca tttctaccat acgctggtct ctttcatgta
    48601 tttctcccca ataatcggga tgggaaggat ctgttccagc ttttattcct gatagaatta
    48661 atttcaacca tcgttcatct aattggtcaa ttgcgggccc cgctccccat aaaacccgag
    48721 agaatccttc cattcctgca attttatttc catatcctac cccttgatgt atataatcaa
    48781 tttgtgcgcc acttgctcga taaaacggct ggagtggttg atttatcttt ttaaataagt
    48841 caaagtaatc ctgtctggtc cttaatagtg gtactggatg tttcattgta ttttctcctc
    48901 tttttactac caaatagcta attccgactg ttctaatcga agtaagcctt caataaaaaa
    48961 ataatcagaa tagatcagtg gcacattata agtcagttct ctatggtatg cttctgctcc
    49021 gcctccaatg atcccatctt catccatatt ccaatttgca tagtgctttt ctgtcttttg
    49081 gagtatattg atagcagctt catgatacat ttttgatcgt acagaggaaa catatcctgc
    49141 taattccaga agaccgcatg cagcgcatac tccagcactg gtatcaatat tcgaatcttt
    49201 tacagcaaaa tcaatgagag gaatataacc agttttttcg attttctgaa taaaatttac
    49261 tgctatcttt tctgctatag ctagatagta cggatttttt gtgtaacgat aacttaacgc
    49321 gaatccatat aaagcccatg catttcctct gctccaacaa gaattctcat tgtatccttg
    49381 cccgccaagt aaccgaatat atttccctga ttctgcatca aattctgcga tatgcccggt
    49441 tgatccatct ttcctcacta gatacttagc tgttgtatct gcatgctgcc gagcaatttt
    49501 agagaacctc ggatcaccag tttccctcga agcccaatac agtaatggta aattcatcat
    49561 actatcaata atcatccaac caggtttatg gtcattccat gctactaaat agttccctga
    49621 aatattatag cgtccagcaa gtaccgtagc tgcatgtaac cctagttgtc gactatgttt
    49681 gttttgagac tgttgataat gtgcaatagc cgatggtaag aataaaaaac ctacatcatg
    49741 atgaacgtct gaaaaatggt acagggcaga ttctaataat tgttcttgtt gtattgctgc
    49801 ctctttgaac ttttctcgtt ttgtcttgtg aaagagctgc cataaaatac cactccaaaa
    49861 accattggtc caccagtcga ttcctctgtc ctttattacg tctgtatatt ttccgttgta
    49921 agaaatatat ggaattttat tccccattct gtctaattcc gcatctatct tttcagaagc
    49981 tacattcaaa atttttcttg accattcctt cgtctgttca ttcatggata tcccgccatt
    50041 cttttaattt ttatgaatgt ggagttgctc ctagaacgaa ctagatggtt aacattcgtt
    50101 atttttttta cttatttgca gctccaatgt cattctcctt aaatttagac tgcgcccaca
    50161 cataaatcaa gaccattggg atcactgaag ttgctaaaat tagcataatt gcgccataat
    50221 taattggctc acctgtacta gaaaccgtca tatttttgat aattaaggga accgtatatt
    50281 tatctttatc tgaaagcatg actaatggaa atagatattg attccaactt cccataaaga
    50341 taagtaaacc actagtaata atcgtaggtg ataaaatcgg tacagcgatt ttcgtaaaaa
    50401 tcacccactc attggcacca tcgattctgg cagcctccat cagtgctgta ggaaattgac
    50461 tgctatattg tcgcatcata aacactgaag ttgtactggc aatcgaaggg atgatcacac
    50521 ctgcataagt attcgataat cctaaacgac tcataatgta aaataaagga atcaatgtgg
    50581 taaaaaaagg caccatccta gatagcataa ctgtattgaa caacagttta ttccccataa
    50641 aacgatactt tgctaatgca taacccgcca ttgtagaact ggctgtagct aaaactgtac
    50701 tgattacagc aataacaatc gaattccaaa taatcaaggg ataattatat ctcgccatga
    50761 tggcttgcca gttttttaat agcatatccc caaacttgaa attgattccg ccacttaaaa
    50821 tttcttgatt actttgcgtt gaagctacaa tcatatataa atatggcatc aaaaatatta
    50881 aggcaccaaa cacaataatg acgtacgtaa aaattctgct ttgtatcttt tgtttagtca
    50941 tttttatcac ctccgaattt aaattgaata gccgataata taacagatac aaacaccatc
    51001 gtccaggcaa tagcagaggc ttttccaaaa tcaaccattt caaaacttgt tttatataaa
    51061 ttgactccta cgaccaaagc tgaattttct ggtccattgg aagtcccaaa gaatatattt
    51121 gggatattga aattacccaa gccattgata gtggctaaaa ctgtggccat taaaatgatt
    51181 ggctttagca tggggatcgt taataaaaag aattgccgaa atttcgatgc tccatctatt
    51241 tcagcacttt cgtatatctc tttagaaatc ccttgaagac cagctaaaaa taaaatagtg
    51301 ttatatccta cactaagcca aatcatcgtg atgatcattg atattcgtat ccaaaaagga
    51361 tttgttaacc actgtacacc gttaattcct agcgcattca aaaatccatt gaataaccca
    51421 gtcggattga aaaaagtcat gaatacggaa gctacagcca ccgttgatgt aatggttgga
    51481 atataataga cagttctaaa aaatcctttt ccttttaact gtgctgaatt caataaaaca
    51541 gcaaagtaaa tagctaatag catcgttatt ggaattgatc ctgccatgat aatcaaaaca
    51601 ttcgtgtaag atttaataaa caatggatct cggaagatag ctagataatt gtcaaatcca
    51661 acaaattcaa atgagtagcc aattttcata aaactaaaag caaacgagat aatgatggga
    51721 atcaaaatcg tcacaataaa gaaaataaca aaaggtgtga ccaatccata agcacttttt
    51781 ttatgagacg tatctgttgc ttttttcttg ctgttcttta ttgtcttgct tggtaacgaa
    51841 gattgcatca tcgttaagtc ctcctaatat tttttaatca ccagttaatt ttacgccgta
    51901 cttatgagca aaattttctg cttcttttag atatgaattt tcaaaatcac ctgtttgcca
    51961 atagttatat gcagcggtag tcaaataaga cgtagcatct gaataagtgg ctggtaaaac
    52021 agtatttgat gtattttcat tgattttatt caatagttca tagtattttt gtcctcctta
    52081 gtagtcaaac tttttctcag ctgcttctgt ttgataggcc aatgagttag atgtagccac
    52141 gcccagctcc agtgcgttgg ctaagcattc ttcatctgtc agcataaatg taagaaattg
    52201 ttgtgccact tgtggatttt tggcatcttt agggacataa aaacttgatc ctcctgatac
    52261 tgcacctttt cctggatttt cttttgaaaa aggaatcagt ggagccatgc gccatttgtc
    52321 agatgcatca ggaaaattca aagacatatt cgtagcatac cagccgccag caacaaacat
    52381 tgcagattct tgaaatgctt tctcagcatc ttgtgcaccg tagaaatgta caatatctga
    52441 gtcgatcatt tctttgatta actttgcagc atgtttcgct tctttagtat tcagacggat
    52501 atttccttct tcatcaagca aagattgatt ctgctgggct agcatgttga taaacaattc
    52561 ttggtcccca gattctggca aggcaattcc aaaactacca gttttttctt ttaatgcttt
    52621 tgccacctgg atatattctt cccaagaact gatttcttca aaatctacac ctatttcagc
    52681 aaattttttt ggttgataat aacaaagtgc tggggtaaaa tcactagccc aaggaataat
    52741 cgtttcatta ctcgtttgtt gacgcaaaat attcaatttt tgatcataaa actgatcgcc
    52801 atatttgtca taaaatccat attcatcagc actataaaaa gcatcttgaa atttttccag
    52861 cacacctgct gctgaagtgt ctgccatgga gataatatcc ggcatatctt cgtgtgttac
    52921 caattttggg gtgatcattt gtactgttgc tgttcccgaa gctacattca caatttttaa
    52981 gtcaatattt gctccagttt tgcggttata gatttttgca gcattttcaa catagtttgt
    53041 cagtttgccg tttgcgacat atacttctaa tgtagtggct ttttttatca ttggatctgc
    53101 gatgtttgct gttacttctt cgttagtttt tccacatgca cttaagctga ataatagggt
    53161 aatgtttagc aaagctatca ccaatttgta tctttttttc atgtctatcc cttctttctt
    53221 ttcaagttct tatataaaac gctttcactt atgtatatta acggaattag taacacaaat
    53281 atttatataa aaaaatattt tacttgtcgt tttggctttt catttttgtt ataatagtca
    53341 ttaattataa atagaaagct ttttatataa aaagagaaag gaaagtggtt cttttgttaa
    53401 caaacaacca atcctccaat cttgtcaaaa aaacactaac ctattccata gatacccatc
    53461 ttcatttatc tgctgctccc tattttcgac tactaataaa tgaaagcgct aatttatatg
    53521 ctgaatttgc acataaatct atagaactag ataatccttc ccttttattc attgataaac
    53581 agcaagaagt ccaaatcttt tctactcaaa aggaaacaca aacttttatc attatccagt
    53641 tttattttga tgagcaatta ttagcacata ttacgggaca atctttttct atagatcaag
    53701 aaataacatc tatcattact tttctgaaaa atgaacagtt caaaattgat tatctaaaac
    53761 atagtaagcc agctatttcc tatgatcagg aaatcaattc ttttttcata ctatgccaac
    53821 agatgagcat tcatttgctt tccttattag ttacttattg tctgcaaaaa attcaattgt
    53881 cttccttacc aaggcattat caacaggaac ttgctttatt gacaatacct tcaaatgaga
    53941 aagtcattcc taaaaaaaag catattcttt attcagatga attagttgat ccaatcatac
    54001 gatatttaga agaacatctt tcagaaaatt ttttgcttga acaattagcc caaatttttt
    54061 ttatcagtcc agccagctt
//
LOCUS       DAYYOA010000007        50599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000007 DAYYOA010000000
VERSION     DAYYOA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.007"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..524
                     /locus_tag="ACHBKA_000392"
     CDS             <1..524
                     /locus_tag="ACHBKA_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM9444111.1"
                     /translation="YESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNI
                     EEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGV
                     VFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDK
                     DGVSQAFERILTI"
     gene            805..2025
                     /locus_tag="ACHBKA_000393"
     CDS             805..2025
                     /locus_tag="ACHBKA_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9444112.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2022..4742
                     /locus_tag="ACHBKA_000394"
     CDS             2022..4742
                     /locus_tag="ACHBKA_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9444113.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4726..5670
                     /locus_tag="ACHBKA_000395"
     CDS             4726..5670
                     /locus_tag="ACHBKA_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9444114.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5739..6749)
                     /locus_tag="ACHBKA_000396"
     CDS             complement(5739..6749)
                     /locus_tag="ACHBKA_000396"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9444115.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6929..7273)
                     /locus_tag="ACHBKA_000397"
     CDS             complement(6929..7273)
                     /locus_tag="ACHBKA_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9444116.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7266..7700)
                     /locus_tag="ACHBKA_000398"
     CDS             complement(7266..7700)
                     /locus_tag="ACHBKA_000398"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM9444117.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7838..8575
                     /locus_tag="ACHBKA_000399"
     CDS             7838..8575
                     /locus_tag="ACHBKA_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444118.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8568..9779
                     /locus_tag="ACHBKA_000400"
     CDS             8568..9779
                     /locus_tag="ACHBKA_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444119.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9983..10765
                     /locus_tag="ACHBKA_000401"
     CDS             9983..10765
                     /locus_tag="ACHBKA_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM9444120.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10835..11497
                     /gene="trmB"
                     /locus_tag="ACHBKA_000402"
     CDS             10835..11497
                     /gene="trmB"
                     /locus_tag="ACHBKA_000402"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM9444121.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11595..13349)
                     /gene="ade"
                     /locus_tag="ACHBKA_000403"
     CDS             complement(11595..13349)
                     /gene="ade"
                     /locus_tag="ACHBKA_000403"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM9444122.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13481..14410
                     /gene="comGA"
                     /locus_tag="ACHBKA_000404"
     CDS             13481..14410
                     /gene="comGA"
                     /locus_tag="ACHBKA_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM9444123.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14367..15416
                     /gene="comGB"
                     /locus_tag="ACHBKA_000405"
     CDS             14367..15416
                     /gene="comGB"
                     /locus_tag="ACHBKA_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM9444124.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15413..15691
                     /gene="comGC"
                     /locus_tag="ACHBKA_000406"
     CDS             15413..15691
                     /gene="comGC"
                     /locus_tag="ACHBKA_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM9444125.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15666..16136
                     /gene="comGD"
                     /locus_tag="ACHBKA_000407"
     CDS             15666..16136
                     /gene="comGD"
                     /locus_tag="ACHBKA_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM9444126.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16108..16455
                     /locus_tag="ACHBKA_000408"
     CDS             16108..16455
                     /locus_tag="ACHBKA_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444127.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            16418..16843
                     /gene="comGF"
                     /locus_tag="ACHBKA_000409"
     CDS             16418..16843
                     /gene="comGF"
                     /locus_tag="ACHBKA_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM9444128.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            16815..17174
                     /gene="comGG"
                     /locus_tag="ACHBKA_000410"
     CDS             16815..17174
                     /gene="comGG"
                     /locus_tag="ACHBKA_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM9444129.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17346..18353
                     /locus_tag="ACHBKA_000411"
     CDS             17346..18353
                     /locus_tag="ACHBKA_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9444130.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18378..19562
                     /locus_tag="ACHBKA_000412"
     CDS             18378..19562
                     /locus_tag="ACHBKA_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM9444131.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19999..21582)
                     /gene="abc-f"
                     /locus_tag="ACHBKA_000413"
     CDS             complement(19999..21582)
                     /gene="abc-f"
                     /locus_tag="ACHBKA_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM9444132.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21687..21815)
                     /locus_tag="ACHBKA_000414"
     CDS             complement(21687..21815)
                     /locus_tag="ACHBKA_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444133.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22160..22303)
                     /locus_tag="ACHBKA_000415"
     CDS             complement(22160..22303)
                     /locus_tag="ACHBKA_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM9444134.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22525..23004)
                     /locus_tag="ACHBKA_000416"
     CDS             complement(22525..23004)
                     /locus_tag="ACHBKA_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9444135.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23077..23361)
                     /locus_tag="ACHBKA_000417"
     CDS             complement(23077..23361)
                     /locus_tag="ACHBKA_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444136.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23439..23633)
                     /gene="ssrS"
                     /locus_tag="ACHBKA_000418"
     ncRNA           complement(23439..23633)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHBKA_000418"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23705..24991)
                     /locus_tag="ACHBKA_000419"
     CDS             complement(23705..24991)
                     /locus_tag="ACHBKA_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM9444137.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25129..25800
                     /locus_tag="ACHBKA_000420"
     CDS             25129..25800
                     /locus_tag="ACHBKA_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM9444138.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25766..26254
                     /locus_tag="ACHBKA_000421"
     CDS             25766..26254
                     /locus_tag="ACHBKA_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM9444139.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26267..27214
                     /gene="prmA"
                     /locus_tag="ACHBKA_000422"
     CDS             26267..27214
                     /gene="prmA"
                     /locus_tag="ACHBKA_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM9444140.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27215..27979
                     /locus_tag="ACHBKA_000423"
     CDS             27215..27979
                     /locus_tag="ACHBKA_000423"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM9444141.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28212..30425
                     /locus_tag="ACHBKA_000424"
     CDS             28212..30425
                     /locus_tag="ACHBKA_000424"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM9444142.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30436..30882
                     /gene="dtd"
                     /locus_tag="ACHBKA_000425"
     CDS             30436..30882
                     /gene="dtd"
                     /locus_tag="ACHBKA_000425"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM9444143.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31053..32312
                     /locus_tag="ACHBKA_000426"
     CDS             31053..32312
                     /locus_tag="ACHBKA_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM9444144.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32401..33348)
                     /locus_tag="ACHBKA_000427"
     CDS             complement(32401..33348)
                     /locus_tag="ACHBKA_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9444145.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33742..35034
                     /gene="hisS"
                     /locus_tag="ACHBKA_000428"
     CDS             33742..35034
                     /gene="hisS"
                     /locus_tag="ACHBKA_000428"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM9444146.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35049..36818
                     /gene="aspS"
                     /locus_tag="ACHBKA_000429"
     CDS             35049..36818
                     /gene="aspS"
                     /locus_tag="ACHBKA_000429"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM9444147.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36927..37802
                     /locus_tag="ACHBKA_000430"
     CDS             36927..37802
                     /locus_tag="ACHBKA_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9444148.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38068..39951
                     /locus_tag="ACHBKA_000431"
     CDS             38068..39951
                     /locus_tag="ACHBKA_000431"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9444149.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40026..40310)
                     /locus_tag="ACHBKA_000432"
     CDS             complement(40026..40310)
                     /locus_tag="ACHBKA_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9444150.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40300..41034)
                     /locus_tag="ACHBKA_000433"
     CDS             complement(40300..41034)
                     /locus_tag="ACHBKA_000433"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM9444151.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41163..41786
                     /locus_tag="ACHBKA_000434"
     CDS             41163..41786
                     /locus_tag="ACHBKA_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM9444152.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41978..43099
                     /locus_tag="ACHBKA_000435"
     CDS             41978..43099
                     /locus_tag="ACHBKA_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9444153.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43106..46234
                     /locus_tag="ACHBKA_000436"
     CDS             43106..46234
                     /locus_tag="ACHBKA_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFM9444154.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46253..49447
                     /locus_tag="ACHBKA_000437"
     CDS             46253..49447
                     /locus_tag="ACHBKA_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM9444155.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49593..50300)
                     /locus_tag="ACHBKA_000438"
     CDS             complement(49593..50300)
                     /locus_tag="ACHBKA_000438"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9444156.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16535 a         8874 c        10532 g        14658 t
ORIGIN      
        1 gttatgaatc tcagaattat aaaaataaaa tgaaaaaaat ttttcacgag gtagggagtg
       61 aattttctat caaagagcta aactatgagc ttgagtatga aaaatctatt tgttcaatcc
      121 cttataataa tattgaggaa attatagaaa aggatgctat cagcatttac aaatttttta
      181 tttttattcc taatcgtcaa ttgaaagcta atatatttaa ccatttgaaa aagatgctaa
      241 atgtgtctgt tactgaatcg gcacctgtaa atattgaaat cgtacctcaa aatgtatcta
      301 aaggtgtggt gtttgatcgt ttggaatcaa tctacaattt aaagaaacct ttgagaatcg
      361 cgatcggaga tagtttaaat gacttatcta tgtttgaaag tgcagatatt agttttgcaa
      421 tgggaaatag tcatcaagaa atcatgaaaa aagctgatta tgtcactgtt tcaaatgata
      481 aagacggagt atctcaagca tttgaaagaa ttcttaccat ataaactaat taaggtagta
      541 tagaacatag aattctaaat acggaaaaat ggagcataaa ttccagtaca aatagtactt
      601 cttcagtgtc aaacgaagcg aatataacat agatatttag gattgtgaca aaaatcatgt
      661 cacaatcttt ttttcgaata aacttatttt ttgaagtcgg ccatttatcg taatttcttt
      721 gcataatacg aacaaatttt cggttattga tgaaaaaaaa cattgaaaat ggtatgataa
      781 aagaaaaaaa ggaggaaatt tttaatggtg aaatttatcc atgcggcaga tttacatatg
      841 gatcggtcat ttgaaggact gacaactctt gataaaactg tacaggaaaa actattgaag
      901 acgaatctga ccgttttatc aaatatcatt gaacaggcaa taataaataa cgttgatttt
      961 gttttacttg caggagataa ttttcatcaa aatcgtcctt cattaaaaat acagaaacat
     1021 ttttcagaac agatgaaacg gttagaaaag aaccatatac ctgtatttat aatattcgga
     1081 aatcatgatt tctatcaaaa agagcggtat tggttctcct ttcctaaaaa tgtccattta
     1141 tttacatccg aaacggtaga gacaaaaaaa ataacgataa aaagtggaga aactgtctct
     1201 ctcagtggat ttagttatcg tcaaccgtgg attcaaaaag acaaagtgat ggaatttcct
     1261 tctagagaac tcacagatta ccatattggg ttgtaccacg gggaacctgg cgtctctcaa
     1321 aaaggaaact atgctccttt ccaaccctca aagatgcaag aaaagggata cgattattgg
     1381 gctttagggc atatccatgt gccaacggtc ttaaatgaaa agcaaacaat tgtttatcca
     1441 ggtgcgcctc aaggtcatac taaaaaggaa caagcttcta catctattct actagttgaa
     1501 ttaagtaaag gaagttgcca aatacatccg attaaagtag cagaggtcta ttggaagaca
     1561 aaggaaatat cattgaggaa agcaagaacg acaaaagagg tcattgttca cattcgtcag
     1621 cagctttcaa aagtagagga tggatttagt ttaattgaga taaaattaaa agattatgac
     1681 catctcaata cagatgtttt ggaacggata caatcaggag agctattaga ctatttgtta
     1741 gaggaattct cagataggat aaatgatttc tttatttggc gtatttctct catagagaat
     1801 actttttata cgaagacccc acttgctgct tctgctaaat tgatggaaca gttatttcaa
     1861 tactatcaga cacctcaaga tttccaacaa attttaaatg aagtatatag tcatcaagaa
     1921 gcggctagaa tattgagcga actgccggaa tatcaagaag aaacacttga aaaagccaag
     1981 cagctgctta atcaagattt tttatttgag gaggatcagg aatgaagatt ttaaggatcg
     2041 agattgctgc ttttggaaaa tggcgtcaaa aaagttttga cttttattca ggcaatcagc
     2101 tgatctatgg gggaaacgaa gcaggaaaat caacgattta tcaatttatt caagcaatat
     2161 tatttggttt cccttcaaaa ggccgtaaaa aaaaggatta tacaccaaaa gacggctcag
     2221 cctatggcgg caaaatatgg cttaaacatc ctgtatatgg tgaatttgct gtcgagcgct
     2281 acaagcagca aaatcgcgga aaatcgaagg tgtggctggg ggatcaagtg ggaagtgatg
     2341 agttactaga aaaactgatc gcacctttga cgaaagagtt attccagcag gtatttactt
     2401 tccagcaaga gcagttgatg gaactagatc atttgagaga aaaagagcta catgatgcgc
     2461 tgatttcttt aggcatcacg ggcagttctc aagtattcca aaagcggcaa gaatattatc
     2521 agaaagcaca acaattattc aagaaaaaag gccaaaaatt actaatcaat caaaaattaa
     2581 atgaatggaa agagctgcaa gaaaaaattc atcaaaaaca agaacaggaa gcttattttg
     2641 gcaagttgat ccaacaagaa caggaatacg agtcgcaaca gcagcagtta ttggcacaat
     2701 taaaagaggc gaacaaacaa ttttcattag ccagacagca agaacttaat ttttctttat
     2761 atgaagaatg gcaatctctc aaaaaaaaca gcactacagg tcttcctgat gagcaacttc
     2821 ttttagatct ggagtctttc tccaaacggt atcagcaaac aaatgagcgt ttggaagaat
     2881 tagaagcaac actggaaaag catagcggga tggatcagca gtcagccaga tattattttt
     2941 atttagagca ggagcaggaa ctaaaagatc tccagcaact tcaatttaca gcggaacaat
     3001 taaacgaaga atggcaaaga ttacaaattg agactagaga aacaaattca gaattgaaga
     3061 ttttggaaca aaggtggcat tggcagaaaa atccgttacc ggaagaattt ttatatgaag
     3121 aagaatggaa gcatcttctg gctgaagaaa gaaggattgc tgatctagaa agccagattt
     3181 ctgtgcgtct ccaaatagct aaagaacagc aagagatgat tgaagcagaa gtgaatgact
     3241 tggaagagac ctatccagca ttattacaaa atcagtcaga gatagcacca gtctcttcat
     3301 ctaaaccgta tatgctgatg gctggcggag caataatcat tgcaggtata tttgtaccaa
     3361 tgcccttgaa agtgatcctt ttactaattg gtgcaggcat gattggctat ttcttctaca
     3421 aaaggcaacc aaaaattaaa actaaaaatg atttctctga gataaaagga atgtggcaag
     3481 aaaaactagg acaacttgat ctagccttag ctcaattagc tgaagcagaa gaaaaacaaa
     3541 agagaactgg agaagaaaaa cagattcttc agcaagaagt atatcgtttt gcggatcatc
     3601 ggcacttagg aaagatggat acgctcaaac tcatgaaaga atatggacag gaagtcatag
     3661 attatcagaa attatatggc gtatttgagc ggaaacataa aagagaacag gaaatacaag
     3721 aaaaactacg acaaatcgac caaaaatttg agtttgttaa agaatggtta ccgatacaag
     3781 ataagacaat ttcagaaaaa atggtattgc tttcccgttt tatacaagag atggaacaga
     3841 tgaaatttgc cagaagctat cagcaaaata ctttattgaa gcaagaaatc aatcaattga
     3901 aaaagaaaca aaaagtatgg atacaagaat acagcgaaca attgactcaa gcaggcttat
     3961 cttatccttc ggaagtacct gtctttttac aaaaagccaa acaagccgaa cagcagatga
     4021 aaagatacaa tgaattatcg gaaatgctta ctccactatt tcctgaagag ctgacaaaag
     4081 agaaattatc tgtaagactg cgccaactga aagaaaagca atcccaactc cagcaagaag
     4141 aaaaagaagc aagtgaaaat cggcaacgac tccaacttca agtagaagag ttgcaaagag
     4201 acggcacatt ggacgaactt tatcaacaag ctagccggca aaaagcaaaa ttacaggaat
     4261 tactggagga atggggagca cttgaaatcg ccgggacatt cttaggtgat ctttcaacag
     4321 agatgagtga aagacaatta ccccagttgt taagagcagc aggtgattat ttaggtattt
     4381 taacaaaagg aaattatcag aagatattac tgaaaaatga tacactgact ttaacagacg
     4441 gatatacaga atttcccatc tatgagttat ctacaggtac gaaagatcag ctgattatgg
     4501 cgatgagatt tgcttacctc tcattagaag ctaaccgttc gatatgcccg atcatcattg
     4561 atgatggctg gcttcattat gatcatcagc gaaagtatca attagctgaa cttttagcta
     4621 aatttggaga aaagtatcaa ataatttgtt tttcttctga taaagaaatg gtaagctatt
     4681 accatgagat gaaccaaatc gtaaaacaat tagaaggagc aaataatgaa aaaaattcgt
     4741 gatttagtaa tcgatgagca gtttgaacaa tttgtcctga tcaaaagtgc agatgtaaga
     4801 atcgctaaaa acggaaaaaa atttattgct tttactttcc aagatacttc aggtactatc
     4861 gatggtaaat actgggatgc ttcggaggag gaaatcaata aatttacagc cggcacagtc
     4921 gtttttctaa acggtaaaag agaggtttat caaggaaacc ctcaagtaaa gattcttcat
     4981 atgcgcttaa caagggtaga tgagccagga gagcctagtt tatacatgga aagagcccct
     5041 ttgaagaaag aagaaatgga agaagaaatc aatcaattgt tgtttgagat caccaatgcg
     5101 cattggaatc gtattgttcg ctatctactt aataaatatc aaaaacaatt ttttgagttt
     5161 ccggcagcaa aaagaaacca tcatgcattt gccagtggat tagcttatca tactttgaca
     5221 atgcttcact tagggaaatc aatcgccgat gaatatcctg agctgaatcg agcattgttg
     5281 tatgctggta tcattttgca tgatttagga aaggtgattg aactttccgg tcctatgtcg
     5341 acagaatata cgcttgcagg aaatttgatc ggtcatctgg tattgattga cgaagaaata
     5401 actaaagcct gtcttgcgtt gaaaatcagc gaagctgacg aagatgttct tgtattacgt
     5461 catatggttt tagcacacca tggcttatta gaatatggct cacctgtacg tccaagaata
     5521 atggaagcag aaatattaca tcaaatcgac aatatcgatg cttcgatgca gatgatgttg
     5581 acttcgattc gtcaaacaga accaggaaaa tacaccgatc ggattttcgg tatggacaac
     5641 cgaagtttct atgtaccaaa agatttatag caaaaattta tttttttcat tcaaaacaaa
     5701 aaaagcgaga cataagtctt cgcttttttt gttttctttt attttgatga atcagttgtt
     5761 tcttcagttg aagattcttt tgttgaatca gttgattttg tgtcagaact ctttgttgaa
     5821 gctgtagttt cacttgagtc tttcgtactt gatgaagttg ttgtgaatgc actcaacaca
     5881 ttttcaaagg catcatcttt gatttttaca tttgcatctt ttaattcttc accgattact
     5941 ttagttgtga atgcattgtc acttagttta gtttcagtag cgatatcttt tagttgatct
     6001 ttgtatttat ccatgtcatt gcctttattt tggtttttca ccattttgac aacgtagtat
     6061 tctgtcgcat aagaagtagg atttgttgta gtaataacat cggagatctc accgtctttc
     6121 aatttgaaag ctgcttcttt tacttctgca ggaatagttg ttgtcgttga gtcgaattta
     6181 actttaccgc catcttcttt tgtctctgta tcagttgatt tgtctttggc taatttagag
     6241 aaatcatctc catcatccgc tgattttttc acatcttttg cttcatcttc acttgataat
     6301 ttgatgattt gtgcttctac ttctggatgg aaagatttcc aagcagtttt caaatcgtca
     6361 tctgtaatat ctacgtgagc tttcaagcca gcttcaaaag ctaaattgtt acgaatatat
     6421 tctttataag tatctttcgt atatccagca gcttctaatt gtgattcaaa agtatcacct
     6481 aatgatttcg cttgtttgtc gtattctgca tccacttgct tatcggttac tttatcgcca
     6541 tatttgttat tgaatacttt ataaatgatc atccgttgta ctaaggattg gttcgatgat
     6601 tcaagttttg cttcatcata gaaatcagat acagtgatcg ttccgccttt catcgttgcg
     6661 atatctttat tagtatcgcc tgaacaagct gctagagtta aaacagccaa cgcactagta
     6721 gctgccaaaa taattgattt ttttttcatt aaaatttgca ctcctgtaat tagttattat
     6781 ttttatatat taactatacc atatcccatt tcctttaaaa acgttgtcgt ggtagtttca
     6841 aacaaaattc aaaatttcat cacattccta ggaaatacgc aaacttttct cacttatata
     6901 gttaaatctg ttgagaaaat aatcattatt atatttgttc aggatactct ggcagatcag
     6961 cttttagttt ttctacttgt tcattgattt gtgtgatacg tggttcggct tggaatttaa
     7021 aatcttcgat atcttttttg attgcttctt ggaaagcagg aatcacagat tcagctgttt
     7081 cttttgtttc gatcaatgcg cttttaaaat gattcaggct gttatttaaa tcatttgtca
     7141 gagttgttgc ttcttccaat tcttcgatca gtttctttct tgtttcattc ccgctcctgg
     7201 gcgccaataa cagacctccg acagtgccag cagcagctcc aaataacagt cctttgaaaa
     7261 agtttttaat catcgcagga cacctgcttt acgattgttt cttgaatagc ttttagttct
     7321 tccgatccat aactatcttg atgattgaca aagtgaatag aaaaatcatc ctctttgctg
     7381 tagcgaggga ctaaatggat atgtgaatga aaaacagatt gataggctag ttcacggttg
     7441 ttattcaaga tattaagacc tttcatttct ggaaaagctt tttccaatgc ttgagcaatc
     7501 ttaggcacac gcgaaaaaac ctctcctgca agttcgggat catattcaaa aatatctgct
     7561 acatgacgtt taggcacaac taatgtgtgt cctttcgttg cttgagagat gtctaaaaaa
     7621 gcatagacct tgtcgtcttc atatatttta tagcttggga tttcttgatt gatgattttg
     7681 caaaaaatac agttttccat ataaaacatc ctttctataa agaataaata cttcagctta
     7741 actttaccat aagttaaagc tattgtaagg aaaaaaacaa aataccgtca ttttcttcgt
     7801 ggtataataa ataaaaataa atggcggagg aaaaagaatg agtttaatta tcgatcatgt
     7861 cacagggggg tacggacatc tccctgtgtt aaaagatatc agtttcgatg tacagcctgg
     7921 agagatagta gggttgattg gactgaacgg agcagggaaa agtacgacca tcaagcacat
     7981 tatcggtctg ctggaagctt caaaaggaac gatagaaatc aatcatctat ccttaaagaa
     8041 aaatacggaa gaatatcgca aacaaattgg gtatattcca gagactcctt ctttatatga
     8101 agaattgaca ttgagagaac atatcgaagt cactgcgatg gcctacgata tacctatgga
     8161 tatagcaatg aaaagagcag aaaagttgtt ggagactttt cggttatcta atcgtcttga
     8221 ctggttccca gctaattttt caaagggaat gaaacagaaa gtgatgatct tatgcgcctt
     8281 tttgatcgag ccttctttat atatcatcga cgaaccattt ttaggcttgg atccactagc
     8341 tatcaatgca ttgcttgaat tgatggtaga aatgaaaaaa gaaggagccg gtattttgat
     8401 gtctacgcat attttagcta ctgcagaaaa atattgtgat cgttttgtgg tcttacataa
     8461 cggagagata cgagcagacg gttctcttga gatgttgaga aaagaatttc agctgccgga
     8521 ttcttctttg gacgaaatct atattgcttt aacaaaggag caggcacatg agtagttttt
     8581 ttcaaaaacg tttagcaaga catcaaaaga aaatgatgaa atatatgcgt tatgtattga
     8641 acgatcattt tgttttagtt tgtcttttct tgattggcgg tgtgggatta tattattcta
     8701 attggttgaa aaccctgcag ccaccgtttg caattggagg gctcataatc tctgtatttt
     8761 ggatgctctg tttattcgtg ggtaagatgg ctacttttgc agaggcagca gatattgttt
     8821 ttttacttcc taaagaaaaa gaaatgcaaa cctatttgga acgagctttt cgctattctt
     8881 gcatttttcc gttgatcttt ttgatattgg catgcggatt ggccatgcca ttagtggtgg
     8941 ttagtacgga gcaatccttt tcgatgtttt tcgtctatct gatcatatta tggagtttga
     9001 aattcagtca tctgcaaatc aagagagcag aattgtttcg actagatcaa caagttattc
     9061 gttattggaa attaggctgg tttttttctt ctctgatctt tttgtttcta tcattttatt
     9121 ggctaatatg ggcaggaata gcaggtgcat tagtacaggc aagtctatat tactggtttt
     9181 tgtggaagca acaagaaaaa ggattggatt gggagaaaat ggtgcatgta gaagaacata
     9241 ggttgcatca gatctatcaa tttatcaatt tattcacaga tgttcctgaa attacggcaa
     9301 agatcaaaag aagacgctat ttggatccta tattgactca tattaaaaga gaatcaaaaa
     9361 acacttatct ttacttgttt gcaagaagat tggcaagagg ttctgatttt agtggtcttt
     9421 atttccgatt agtaattata ggtgggttat tgattgccgg agtagtcgac ttttatttta
     9481 gtctgattat cggtttctta tttttatatt tgatcggttt ccaactgtta cctttagcga
     9541 accagttccg ctatatggtt ctcgttcagc tttatccaat ccataagaat cagaagaagc
     9601 aggcgattca gtatctactc acatgggtat taattatcgc gtcggttatt tttggtttga
     9661 ttgctgcagt cgtattagat gggcagcagc gatgggttcc gctagcggct tatttgattg
     9721 ttactatgct ctttgttaaa ctatatgtac caactcgttt gaagaagatg tcagactgaa
     9781 aacaaagctg tgtagggtaa aaatgagaat gaaaaataga gcgttgacag taagaatgct
     9841 gttcgttcta ttttttttga gaaaagctgg ttttttgaat ggcaattaaa tgaaaaagaa
     9901 cgtatatgtg cttgacgtgt agtgttcagg cgactagaat aataagtact gcaaaacaaa
     9961 gagtaagagg acgggagaat gcatgatgga gtttcagtta gaccaagagt ggcggttacg
    10021 tcccatcaaa ggcgacacag gaaaaacata cataggcatg cgtgatgagg acaaagtctt
    10081 tattaaacga aacacgactc cgatgttggc tgctctttca agggaaggaa ttacaccaaa
    10141 acttgtttgg acaaaacgaa cggggaatgg cgatactttg acagcacaag agtggctgga
    10201 aggtcgcttg ctgactccta atgagattgg tcaaagaaac gacgtgattg atgttttgta
    10261 ccatttacat cattcaaatt ctcttaaaac catgctgacc cgtatcggtg gtcgtgtcat
    10321 gacgccggag aagatgctgc aaaaatatga aaaagaatta ccggaagaac tccagaaaaa
    10381 tcaatttctg caaattgtcc atcgttttct tcgtgagcag atcccgtcaa tagataagga
    10441 aaacttatca gtagtacacg gagatgtaaa ccatcgtaat tggttagtat gtaaaaatta
    10501 tttatatcta gtcgattggg attcaatcat gtttgcagac cctgcggtgg atattgcaac
    10561 gattttagga aattatgtgc cactgtctag ctggaatcag tggctgatca gttatggcat
    10621 acgcccgact gacgaaatgc ttgaaaaatt tcattggtat gcggccatga atttgctcca
    10681 agaaatcacc agatattgca aacgtgaaga tcatcgacgg atgaacgaag aaattttaca
    10741 attaaaacga attttcagtg gctgaggttt ggcccaaact ctgcggagat ttggccagac
    10801 ctttttaatt agaggaaagg aatggagata aataatgcgt gtccgaaatc gtccgggagc
    10861 agcggaaatg ttagcggctc acccgaattt tgtaattagt gatccaacat tgtggaaggg
    10921 aaaatggaat gaattgtttg aaaacgatca tccaatccac attgagattg gtatggggaa
    10981 aggccagttc atcactggaa tggcaaaagc ccatcctgaa atcaattata ttggagtaga
    11041 aatgcaggtc agtgttgttt cgattgcact agacaagttg atcgaacaac cgcttcctaa
    11101 cctgaaactt ctacatgtgg atggatcagc gttgacagag tatttcgctg acagcgaagt
    11161 agatcaaatc tatctgaatt tttccgatcc atggccaaaa aaacgtcatg aaaaacggcg
    11221 attgacatat aagacttttc ttgctgtaga tgaacagatt cttcgtccaa atggagaaat
    11281 ccatttcaaa acagataatc aaggattgtt tgagtattct ctggcaagtt tttctcaata
    11341 tggcatgatc ctcaaacagg tatggttaga tttacatcaa agtcaatttg aaggcaatat
    11401 tatgacggag tatgaagaaa aattttcttc aaaaggtcaa cgtatttatc gagtagaagc
    11461 tcaatttcaa gataagacca aaaagaaaag tctgtgaaac taactttctg ctttcataaa
    11521 aacattaata tccataaata aatctcagaa aatgaacatt acagatgtaa tgtattttct
    11581 gggatttatt tgatttaaaa tgtatttatt tcatcggttt ttccacaagg tggaatcaat
    11641 tggaagcgag aaaatgaaaa taatcctttg tcggtgatct tcaattcggg tatgacagat
    11701 aatgttaaga aagaaagtgt caaaaaagga tcaaaggttt tagctgctcc tagttgatgt
    11761 gctttagtta ttaattcatt caactgttca tagacgatcg aaaaaggttc ctgtgtcatg
    11821 attccgccta taggaagaga aacacaagct atttcctgct gtttgttgac aacggtcatc
    11881 ccgccacctt tttgaatcaa aagattagca gcatacagca tatcctcgtc gtttgtccca
    11941 acgacaacta gattatgcga gtcatgggca atcgttgtcg ctatcgctcc ttgtttcagc
    12001 tgaaatcctt tcactatgcc taatccaatg tttcccgttt gatggtgacg ctcgatcacc
    12061 gcaatcttta attggtcttt ttcgattgaa ggaatgaatc gttcgccttg tcggtccact
    12121 ttttctacca aatctttagt cactagacta ttcgggataa tttcgatcac atgggcttgt
    12181 gaagaagaaa gaagcagctc taacgaatgt ggatttaatg gttgagcgtt tagttttggc
    12241 agacaacacg tatatgaatt tgaatgatca gcaaataaat ggtcttggag ttgcccagat
    12301 tgaacaacta atgttccctt atagaaaact tcatgaattg gcagcttggt aagatcgtct
    12361 gttaagaaga aatcagcttg atacccaggg gcaatcgcac ctagtgtttt caaatcaaaa
    12421 cattctgctg cattcaatgt tgccatctga atggcttgta atggatcgat tccaagtgca
    12481 atagcttgac ggacgatatg atcgattgat ccttctttta ctaaatcatt gatcagttta
    12541 tcatcggtga caaagaagca tcttcttgaa ttttcaggtg tgatcgccgg tagtagcgat
    12601 gccagatctt ttgcaacggt cccttctcga accattaaat acataccaag atccaaacgt
    12661 tctttagctt cttttgcagt tgtagcttca tgatccgtcc gaatacctgc tgcaggataa
    12721 acatttaact cttccatgcc gataccagct gcatgcccgt caattttttt cttatgctgg
    12781 tgcatcaatt ggatcttttc taggatgtct gtttcattat ttgctactgc ttgatagttc
    12841 atgacttcag ctaagcctaa aacttcgggt ttttgtaaaa aaggggctaa tttttttgca
    12901 tttaaatttg caccattggt ctcaaaaggg gttacaggta cacaagaagg tagcataaaa
    12961 aagatattca tgagtgtttg tcttgcatct tctatcataa actcaagacc tgtactccca
    13021 gcaatgtttc ctatttcatg gggatctgtc actacagtag taactccatg aagcaaggat
    13081 acttttgaaa attcgcttgg tgataaaagt gagctttcga tatgcacatg gccatcgatg
    13141 aatcctggca ctagatattg cccattagca ttatgtacgc tttttccttc gtattcaccg
    13201 attcctacga tcattccttc gcagattgct acatcttttt ccatgatttc tttcgtaaag
    13261 acgttgacga tcttagcatg cttgatcact aggtcagcag gctttttttt acttgctact
    13321 tcgatatatt tccttaatgt tgtttccaat ggagtgcctc ccaagataaa tttttataag
    13381 tataacataa attcggcttt tgccaatatt tttttgtaaa ttcattttat ttgaaacaaa
    13441 aagatcaaaa agacgtatgt tttttgtgga ggggagaata atggaaataa aacgattatc
    13501 ggatcaaatg ataagtgaaa gttttgccaa acatgcccaa gacgtatata tattgccagc
    13561 ttcagaagta ttttacatct attttcgcta tggaaaaaaa cgaacattct ggaagaaagt
    13621 ctcttcggca gatggacagc aactgatctc aagatttaag tatttgggac agatggatat
    13681 cggagaaaaa agaaaagctc agttaggtgc atttacttat tctttagaaa aacaggaagt
    13741 tcgtctgcgg ttgtcttctg taggagatta ccttcaacga gagagtttag tgattcggct
    13801 attatacggt acttttgaaa caccccatcg ttgctttagt tctcgagatt ttgcaactat
    13861 tttacagaaa accgtcagac gtggattata ccttttcagc gggacagtcg gatcaggcaa
    13921 gacttctcta atgtatcggt tggctttgga agaaaaattg caagtgatta ctatcgaaga
    13981 tcctgtagag atagaagaac cgttttttct gcagctacag atcaatccga aaatagcaca
    14041 gacttatgat acacttttga aactcgcatt acggcatcgt ccagacttgc taatcattgg
    14101 cgagataaga gatgaatcaa ctgcacatgc tgcggtaaga gcagcactaa cagggcaccg
    14161 ggtttttgca accgtacatg cacgcaatct tgaaggtaca ttggtcagaa tccatgaact
    14221 gacaaagaaa aaagaagagc tatctgaatg tttagctggt gtcatttatc aagaactttt
    14281 ggaagatgcc aatcaccatc cttctgtttt atggagttat caatttatgg aggaagaatc
    14341 agtaaaaaaa tcatgggagg aaagttatga agaagctcag gaaaatattt ggcagtcaga
    14401 actattctaa aggattaaca agaagccagc aaaaccagtt tgcacaaacc atgggagaac
    14461 tgcttttatc tggttttagt ctgcaagaat ccctttattt attactcacg atccgttcgt
    14521 ttccacctgt tattttggca aatatccaac atcagctaaa ggaagggcaa gcgtttagtg
    14581 aggtactttc gcaaatagga tatggagcag agcaactggt acagatcgag ttagctgaac
    14641 gtcatgggaa tctgccgaca actttgttgg aaattgccaa acagtttcgg cttgttgaag
    14701 gatttcgcaa tgatttgaaa aagctgcttg catatccgat gctattgctc acctttttgc
    14761 taggtatctt gtttagtatg cggttgttcg ttttgcctca gcttattggt tcaggaatgg
    14821 tcatgcaaga gcattggggt attcagctga tacaagtctt tcattggtat gttctactat
    14881 tcatcctagc ggttactcta ttggctttac tagtaaggta tcgtctgatg aagttgtctc
    14941 ctgttgatca gagtgaatgg ataagcagac aattattgat tggaccgata tattccttgt
    15001 atcaatcggc ttatttgtca ctagagattg gaaagctttt tcatgaaggt ttcgaattaa
    15061 ggcaaatcat agtctgctta aaggaaacga aacaagaaag tctgattcag catcttgcct
    15121 ctaagttgat tcaaggattg gaggcaggta tgtcattggc tgagcagttc cagaattatc
    15181 gttttctatc agaagaattc agtaaaattg ttttacaggg agaggcaaag ggaagtttag
    15241 gtaaagaact gattttttac agcgaatgga caagaaaaca gcttttcaaa cgtcttcatc
    15301 actggatgca ttttattcag ccaatcattt ttttgggcgt agcggctttg attttattag
    15361 tttatgccgc tgttttatta cctgtttacg gaagcataga ggaggttttg ccatgaaaaa
    15421 gagaaaaata tcttattata gcggttttac attgatcgaa atgctaatcg tcttactgat
    15481 catcagtgta ttagtgttgt tgttcgtccc taatttgtcg cgttatagga atcatgtaga
    15541 ccaagaaagt cgtgaagcga tcatccagtt ggtagacaca caaaaagagc tctatgcgct
    15601 tcaaaataat ggaagagttc ctactgtgga ggaattgttg aatgaaggct atatcaaacg
    15661 agaacatgca gaaatctatc agcggccttg aaggtaaggt tttagcatca ggatatacgt
    15721 tgtttgaatc actcttactg attttattac ttacaacgat cctttctttt ccagttctcg
    15781 cattttctgc atggaagaat gaattggcag tctatcaatt tttcggccaa tttgaaaaac
    15841 gaatctattc tacgcaaaaa atcgctattg tgaatcagat ccaaacaggc ttttattgga
    15901 atagtgaaga gaatcaaatc atttttcatg ttccgaatcc agataaaata aattgggaag
    15961 tattagatat tcctgatgag attaccttga aacgccatgc ttctattact tttgctgcta
    16021 gaaccgggaa tgaaagttca ctaaaagctt atcaatttta ttgggaagaa aaagagcgga
    16081 cgattacgta tcaattccag atagggagtg gtcgctatac aaaaagaatt gaataaaaca
    16141 ggatttgttt tgttggagtc ccttacagcg cttatgatcg cttctgtctg tgtctactta
    16201 gtgagctttg gccagctgtt tttgcttcag gcagaaaaga aaagtgggga ggaactgcta
    16261 tttattcgaa tgatttatga agatatcaag acgcagcgga tgtatcacaa aagaccagag
    16321 cgaagggaag aaatgatccc atacgatatc caaattgtcg ataataatgg agtaatcgaa
    16381 aaagtgtttg tcaatatgga gaaagaagcg atcagtattg aaaaaacaag taattccagc
    16441 atttacgctg attgaatgtc taattggact gatgatattg agcagtctat tgctcacatt
    16501 caatcttttg attcagcaaa ccatccaagt aagtgaattt ttgcagcgta aagatcaaaa
    16561 agagtggcta atatttttag cacaattaga agaagaactc aagagcagtc acaatgtctc
    16621 tgtgaaaaat cagcaactgc actacacgat cgaggataaa cagtatatca tcgaaagata
    16681 tcaggcaatg atacgtaaaa gaagaacttc cggcggacat caacctatgt taacaagtat
    16741 cactgagctt caattattgg aaaaagaaca cacaatttct ttttatgtac actttgaaaa
    16801 tggagaagat ggatatgcca tatggacaaa aaacgatcag tagaaaatgt tataagggct
    16861 ctattttatt ttctgtcttg cttttatttc ttttatgtac gtttttattg atggcagcat
    16921 tagaaagcta ccgtttgtca atggatctta atatacgaat caaaaattat tatatagcaa
    16981 aaataatgaa gcagatggct ttatcggaac agtctacgct tgccgaaagt tcggaaggag
    17041 ttttttacta cacaactgga tctgtaacat atcagtgggt acaacagtcc ctattcttag
    17101 aggtggaagt ctcaccgttt atctttcggt tcacagaaga agtaaaaaat gatacagata
    17161 ctagtacaga atgatggaat gttcgtgaaa acgccctctt ttctgttata acactgtttt
    17221 taataaaaaa atgtttgccg tttcacaaat aaaaaacagt gaaaacgatt gaaattttgt
    17281 caattgttcg atagaattaa acaggtgaaa aaattgttat aatatatcta gaaatgaggt
    17341 gcaccatgtt accagaaaaa atggaacaag ggtttaatca aaacctggaa gctattcagc
    17401 ttttacagaa tgcattggga acttcctttt tagaagccta tgtcgaaaat gcagaaaatt
    17461 taatagatga ttatcaagta cgtgtggtgg atggagtgcc gacaaaagag acaacgcaac
    17521 ggatcacagc tctttatgaa gagttgaaga aactttcttt cgagccagaa gaatggcgca
    17581 gactgtctca attactttta ttgaagggca gccagacaga acatttgcag gcgaaccatc
    17641 aactcactcc tgatagtatc ggttttttat ttgtttttct tattgaacaa ctatacacaa
    17701 ataaaaaagc accggttaag attctggata ttgcggcagg aatgggcaac ttgttattaa
    17761 cagttctttt aaacctatcg aatgcggggt accaaacaga aggaattggt gtggatattg
    17821 atgatacatt gttggctgtc gcagcttcta caagtgattt gacacaagca aatgttcaat
    17881 actttcatca agacgggttg caggaattat taatagatcc tgtcgatttt gcaatcagcg
    17941 atttgccaat cggttattat ccgaatgacg aaaaagcaaa agagttttta acaagtaccg
    18001 aagaaggcca tagttatgca catcatttat tgctagagca atcaatgaaa tatgtgaagc
    18061 cggatggttt tggtttgttt ttgatgccga gtggattttt agaaacggat caaagtgaag
    18121 aaatcaaaaa atggtttaaa gaagaaggtt atcttcaagg aatgattcaa ttgccagatg
    18181 aattgttccg taataaacaa tcgcaaaaaa gtattttgat cttacagaaa aaaggaccac
    18241 aagcacaaca agtaaaagaa gtcttgcttg tgaaacttgc ttcattaaaa gaaccagaga
    18301 aggtgacgga atttttcaac gagtttaaaa actggaaatc atctgctctg taatcattca
    18361 acgagaagga gagtcatatg tctaaaacaa ttgcaatcaa tgctgggagt tcaagtttga
    18421 aatggcaatt atatcaaatg ccagaagaaa ccgtagttgc gaaagggatc gtcgaacgta
    18481 tcggtttaca agattctatt tttaccatca agtacggcga taatcaaaaa ttcgaacaaa
    18541 tcatggatat cgaaaaccac gaagtagcag taaaaatgct gttagatcag ttgattgaac
    18601 tgcaaatttt aagctcttat gatgaaatca ccggagtagg gcaccgtgtt gttcacggag
    18661 gagaaactta tggggattca gtagtgatcg atgagaaagt aatggaccgc attgctgaat
    18721 tagcagaatt tgctcctttg cataatcctg caaacttaat ggggatcaaa gcgtttaaaa
    18781 agattttacc tgacattttg agtgtggctg tattcgatac atctttccac tcaacaatgc
    18841 caaagcaaaa ttatttatat agcttaccgg tgtcttatta tgaagatttc aaagtaagaa
    18901 aatatggctt ccatggcaca agtcatcgtt atgtgtcgga acgtgcggca aaattattga
    18961 acaaaccgat cgaagaacta aaaatcatca cttgccactt aggaaatggt gtatcgatca
    19021 cagcagtcga tggaggtaaa tccatggata cttctatggg attcacacca ttagctgggg
    19081 tgacaatggg tacacgttca ggtgatatcg atccagctgt tctcccatat ttaatggaaa
    19141 aattagagat tgatattgat gaaatgatca atgttttgaa caagaaatct ggtctgctag
    19201 gcctaacagg attgtctagt gatatgcgtg atcttgaaaa gaactatgaa aaagaacata
    19261 ttcgtttagc ttatgacatt ttcgtggacc gcatccgaaa atacatcggc ggttatgtga
    19321 caacgatgaa tggtgttgat gcaattgtct ttactgctgg tatcggcgag aatgacggac
    19381 atgtacgtag tgaaatcatc aaaggtatgt cttggtttgg ttgtgaaatc gatccagcgt
    19441 tgaatgaatt gcgtggtgaa gaagtagata tctctacaaa agaatcaaaa gtaagagtat
    19501 tagtgatccc aacagatgag gaactaatga ttgcccgtga tgtggagcgt ctgcgcggct
    19561 aagccaaagc attcgttagg ataaaagatg aaacgcatgc aagagggaat tcttgcatgt
    19621 gttttttttg cggagaagac cgcaaaatgg taatttaact tcaatttccc ttctttttta
    19681 caggatcttc ttagggaatt ttgcttggaa aagtggtcaa aagggaaaag actggcattt
    19741 tatttattga ttttcaggaa aatcccaata ttttattttc attcatcttt ataagggaca
    19801 ttacatatgt aaaaagaaaa aacattttta gatcagcctc gtgggctgtc gactttattc
    19861 ttcgctttat gtttagctag aaaaccgatc cgaaacattc attgatcgaa taataaaaac
    19921 ttgattttcc acctgtactt tgcctgaatc gtgaaagcac aggtatgaaa atcaagtttt
    19981 ttgttgcgaa aaaacgtcct agtcaatcag ttggccgagc ggctggcggc tgatctgctc
    20041 ctgatcaata acgagtgctt cttggaagtg ttgctgcaaa aagtagcggt catggctgac
    20101 tgctaggagc gttccttgat agtcttgcag catttcttca atttcttctc gagcatagct
    20161 gtcgaggtga ttggttggtt catccaaaat caggacgttg attttttcta aaaagagttt
    20221 cagcaacgcc aaacggattt tttcaccacc ggataaatct ttgatccgtt tacccacatc
    20281 gctttgataa aagccacttt tggctaattg ccgtcgagcg acttcttcgt ttccagtcaa
    20341 tgtgatggcg taagccagca aacggctatc gggatcggca aactgcagct gctgcggcaa
    20401 ataaccgatt tgcaagctac tacctaattt gatggtgcca ccgtaaggct tttcctcacc
    20461 gagtaataat ttgagtaagg ttgattttcc tgcaccattg tcgccgatga tggctaaatg
    20521 atctccacga tagactgcaa aactgctgtc tttgaatagt acttgatcag cgtactgctg
    20581 gtgcaagttt tgaatgatga aaacttcttt gccagatttt ccaccattag acaagctttg
    20641 cagccgattt ttcgggggag gtggcggttt gacgagtgtc agtttcgcca ggcggcgttc
    20701 cagttctttg gcttttttga agaaggactc attgtctcct tcatgtgccc actgacgaaa
    20761 acgacgaatc atgactttca accgctggac ttctttttgc tgcagttcgt aattttttgt
    20821 cagctcagcc aagcgagctt ctttcaagac tcgaaaacgg ctgtagtttc cgggatactc
    20881 aatcaatgca ccttcttcca gttcccatgt ttttgtggtg acttgatcga gaaattgccg
    20941 atcatgagaa acgaccagaa ccgatttttt tgtattctgt aaatagtttt ccaaccagcg
    21001 aatactgtta agatctaaat gattggtcgg ttcatcgagt aaaagtaaat cggcatcggc
    21061 tgctaaaatt ttggctaaag cgactttaac tcgttcgcct ccgctaagtt ctgttaaccg
    21121 tgcttcctga cagtgtggaa tccctaagcc ttgcaagatc gctgcaatcc ggtcttctaa
    21181 tacgtaaccg cctagttctt caaagcgatg ctgcagctca ccgtaacggg ccagacactt
    21241 ctctaagttt gtatctggtt ctgtcatcaa tgcttcgtag cgactcattt gctgtttgag
    21301 gtcctgtatc tcagcggcag aatggagcaa gtagtggttg acggtcgctt cggaaactgt
    21361 aagttcttgt tcgaccgtcc cgatctttaa gccttttttg cgactgataa caccgctgtc
    21421 gacaccttct ctgccggtca agatttgtaa aaaggttgat ttgccagttc cgttgatatc
    21481 gactaaggca attttttcac cagcttcaat ttgacaggat aaatcctcaa ataatggatt
    21541 cccgccgatt gtttttttaa tagaaattgc ttgtatgatc atgatctctc tccttgagac
    21601 cactcaaaaa ggccatggtt atccctggcc tgtgttattt aaactcagat tatcatgaga
    21661 aaaatacctt gagatggcca ttgattttac gtgaattcaa ctgttttgga caaacttgtc
    21721 ccgtcaacag caactgtaaa accaattgca tggctaaatg cgatatggta tcgttggtaa
    21781 ttttcattac ttgttatttt agtcatgcgc atcatagtat tttccctcct tcgtgtagtt
    21841 gtcttttcca gtgtacctga aagtgtcaga aagttcaact agcgctgaca tttacttaat
    21901 atttatcgat gtgtaattct gtcagagagc tttttttatc tgactgaggg taatgtctcg
    21961 tggaccacat cattgtattc tgagctaaac cagcgttcag gcgaaatcat atggtgtttg
    22021 ataaaaaact ttgtctacat gacttccttt tgccatttgc ttaataccgt tgcattaaac
    22081 agctcttctc tttggaaatc tcaccaacat cgcgttcagg agtatcgttg tggttgcaat
    22141 ggcaagcggt agcctttcat caattcttga atgatttacc gaatgtagca cttgccttct
    22201 cgcggtaccc gaacccgaaa taaaatttta gtttggcgat aaaatcggta tccgccatcg
    22261 atacttcata ttttttcgta tcagattcat cggaaacttc cacattgacg aaccaataag
    22321 ctttggcatg cttaggtcgt tttaaaccac accagggcga ctaatcgccc tggtgttcac
    22381 tttttttatt ttacttgaac tcattgactg ttttcccttt attttaaaca ttataaagac
    22441 cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt tttgtgcaaa aaaacctgcc
    22501 gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg tctttagctg gcaattggtt
    22561 ttctaaatca gttcggacaa caagcacatc gcaagctgcg tttctaataa catattcaga
    22621 aacagaacca ataaataaac gttctacagc attcaatcct gtggctccta acataatcaa
    22681 atctacttga ttatcttctg ggatttctct ggcaataatt tgttttggtg agccatattc
    22741 tacgacagag gttacattat tcaagccagc tttcttcgca ttgctttcat aatctgccaa
    22801 tgtttgcttt gccatttcag tagcttgttc tgcaagcatt ccatcaaagg atgaaacggt
    22861 ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt tctccgttat ttcggatagc
    22921 tacattgacc gcctttttaa aagctaattc agcttcatca gaaccatcta cggcaaccat
    22981 gatttttttg tattgttgta acataagcat tcactcctca agtcaatcgt attgtactct
    23041 tttattgtag aacactttag ggaaaaagag aattatttac tgctttggtt cagcaatttt
    23101 ttttgcaaat cggatactga aggtggcaga aacaataatt acgatcagta aaaagaccaa
    23161 ggcaataaag cgttgattga aaaaagctgt taaaataccc attacgccta aaacagcata
    23221 tgcagcaccg taaaattgta ggaatccttg atttttatca tttttgttga tcaatacaaa
    23281 aaagatatcc tgtttattaa ataaataata gctaacgaat aataatacta aagtggcgat
    23341 aatcattaaa atcctaatca tgaacaaatc ctccaaaata agcaacaaat agaaaaagca
    23401 atttgccaag agcaaattgc tttattcttt aaaagatggt aatctccgtt aatgccgttg
    23461 tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg gtcccatggt cgtagcttcg
    23521 aactaccaaa tgataaggca tgttaccagc agtgacacaa gacgggtccc aagatatcaa
    23581 taaaaatgtt cggtcaacac acaaagagga caattcgaac atcacagatt tcccacctct
    23641 atagtagcat atgaatttaa aaagagcaat gagagagctc gcagaaacag aaccgttttc
    23701 ttgtctagtt ttttggagga tgctttttcc actcttttat acggtaatac tgttgaccca
    23761 gagcctgttc atatttccct gtatcttttg gctgataata ctgagcattc ttcagtttat
    23821 ctggtaaata ttgctgattg acccaagcct gatcaaaatg atgaggatac tgataaccca
    23881 cgccacggtt gagtgatttc gcacctttgt aatggctatc tctcaaatga tcaggaacat
    23941 ctccagcttt gccctctcga atatcagcaa gtgctgcatc taatgccatg taggcagaat
    24001 tggattttgg cgacaagcaa agatcgacga cgacgtctgc tagtgggata cgtgcctcag
    24061 gcaatcccaa tttttcagca gctaatacag cgttcactgt acgagccgca gcagcaggat
    24121 tacctaaacc tatatcttca taaccgataa ccatcagacg ccggcaaata cttgccagat
    24181 cacctgcctc aactaaacgg gcgagataat gaagtgcagc atccacatca cttccacgaa
    24241 tcgatttttg aaaagccgaa ataacgtcat aatgagcatc tccatttttg tcatgggtaa
    24301 gagcttttct ttggacacat tcttcaatga tcgacagtgt aagatgaatc cttccttctt
    24361 tatctgaagg agtcgatagt gtagccagtt ccagcccatt caatgcactc cgcaaatcgc
    24421 cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc taactggatt gcttgttgtc
    24481 caaggccgcg ttctttatcc cttaaagcat gttcgactgc caactggatg tcttgttctg
    24541 ttaatggttt gacttcaaag atctgtgtac ggctgcggat cgctggattg atggtaatat
    24601 aaggattctc agtagtcgct ccgatcaaga tgattcggcc actttctaaa tgaggaagca
    24661 gaaaatcctg ttttgtttta tctaatcggt gtacttcatc caataagagg atcactgtac
    24721 cactcatttt tgcttcttcc gctacaactt gtagatcctt ttttgaatct gtagcggcgt
    24781 ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc acttgcgata ctcgtttttc
    24841 cagtaccagg aggtccatat agaatcatgg aagagagcat gcgagcatcg accatgcggc
    24901 gtatgatttt tccttccccg actagatgct gctgaccaac gacttcatcg atcgttcgag
    24961 gtctcatgcg ataagccaat ggctgttgca taggattttc ctccttcttg tttttacgtc
    25021 ccttaattat atcatattta gcaagatgcc tagaaagaac agccttctgt tgttttgttt
    25081 tcatagctta tccaactaat attgtgtatg ataaagtagg tgagaaaaat gacggataaa
    25141 caaaagatag atgaattttt ttggaataaa acgacgccag aagtggcacg ggatttattg
    25201 gggatgtatt tggagcatga cacaccagaa ggaagattag ctggatatat tgtagacgcg
    25261 gaagcatacc taggtccaga agacgaggcc gcccatagct atggtttaag aagaacaccg
    25321 cgtgtgcggg caatgtatga aaaaccaggt acgatctacc tttacacgat gcatactcat
    25381 cggattttga acatcattac tcagccagaa gggattcctc aaggagtaat gatacgtgca
    25441 atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc gtggcggaaa aactggtcca
    25501 gatatcagca acggtccggg aaaattagtg gaagcattag caattcctca agaactttat
    25561 ggtcagtcta tagcagatag ttcgttacgc ttagtttttg agaaaaaaaa gacaccaaaa
    25621 aagatcaatg ctttgcctag aatcggtatc ccaaacaaag gtgtatggac agaaaagccg
    25681 ttgcgttttg ttgtgtcggg aaatccttat atctctttac aaagaaaaaa ccaaatcgag
    25741 aaagattggg gttggaggaa agaaaatgaa aaagaaggat ctattaacat atttagatga
    25801 aataattgaa aaacaagtga cagactatga tgtagcttta gattgggata caaaaaatca
    25861 tacgatcgag atcgttgttc gcttgtttgc agaaaataaa gcacaatttg agattgatga
    25921 tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt gaagacggcg tacttctttt
    25981 taatcctcag aaatctgttt ttgatgaacg caattatcta gcagtaatcc cttatgaagg
    26041 aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg gaatatctaa atgaagtatt
    26101 agctcaaggg caaagcgatt tgcttgattt tttggatgaa gacgatgacg aagctgtatt
    26161 cgagcttcac tgggataatc aacaactagc agaattagta gaagtaaaac agcaaaatga
    26221 agcagatact tatttaccgt acccaagtta ttaaggagtg acagatatga aatggaatga
    26281 agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt gctaatattt tgatggaggc
    26341 aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt gaacattttc agtcggatca
    26401 atatggtgag attttagata aaaatgattt tacatcgctg aaagaaggcg cactagtcat
    26461 ggcttatttt ccggaaacga ctttcttacc ggaaatcttg ccttttatca aagatcgtat
    26521 cacacaatta cctgagtttg gtctagctat tggaaaaaat aaagtaacag ttagtgaagt
    26581 agaggaaagc aactgggcaa cagcctggaa gaaatactat catccggtgc gtattacgcg
    26641 aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca gaagatcctc tagaaaaaat
    26701 tatcacacta gatcctggta tggcatttgg cacaggcact catcccacga cttccttgac
    26761 attgacagca ctagaaacag tcttgcgtgg tggtgaaaca ttgctagatg tgggaactgg
    26821 gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca aaagatgtct atgcctttga
    26881 tttagacgaa gtagccgttc gttcggcgaa agaaaatatg gatatgaatg aaatagccaa
    26941 agatgtccat gtatcagcta acgatctttt gacaggaatc gagattgaaa gtgatgtaat
    27001 cgttgcgaac attttagcag atatcatctt attgatgatc ccagatgctt ggcgtttact
    27061 gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa gagaagaagc aaatggttct
    27121 ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt ttccagcaaa aagactggta
    27181 tgcaattatt ttgaaaaaac cagaggtgga atagatgcag cggtattttt taaaagaatc
    27241 atatgaatca aaagactatt ttgttttaga aggggaaaac tatcaccaca ttgtacgggt
    27301 tatgaggatg gaacccgagg atgaagtttt tttggcattc aatgatcaaa tagccattgt
    27361 tgcagaaatc acggagatta ctgaagaggc tgtttatctg aaagaaatta aaaaagaaga
    27421 aaatcaaaag gaactgccaa gttcgattac catagcgagt ggctatccta aaggggaaaa
    27481 gctggattgg atcgttcaga aatccactga attaggagcg caagcttttc ttggtttccc
    27541 ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt atgaaacgac aacaacggct
    27601 tgaaaagatt gctcaagagg cagcggaaca atcccatcgg cagatcgtgc catcagtaga
    27661 gttattggaa acgaaacaag atttatttga gcggctagat gattatgacg tggtcttggt
    27721 tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat gcagccaaaa ttcttagtga
    27781 aacaaaacca gatagtcgaa tcttagctgt ctttggacca gaaggtggat tatcccgcga
    27841 tgaagtcgat tattttgtag aacacaaagg gatactttgt ggattaggac caagaatcct
    27901 tcgtacagaa acagcacctc tttatttatt aagtgctgct agctatcaat gggaattaat
    27961 gaatccttct ttcagctaac gttcttcatt aataaaagag tatcgacaga aagaattgaa
    28021 aaaatagtca acaatttgta taatagaaac aattatttat acaatggata cgaaaaatcg
    28081 tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa acgtataact gcacggattt
    28141 tgggacaatt atcaaacaga tatttgtccc gattcccgtt ttaccgaaaa aagaaagggg
    28201 gatcgattcc tgtgacaaaa gaagaaattt taacgggacc cggtgtcata aagctcgtta
    28261 gtcaatacat gggtcccgaa catgtagcat ttgttgagaa agcatgcgaa tacgcgactg
    28321 ctgcacatga cggacagttt agaaaatcag gcgaacccta cattattcat cctatccaag
    28381 tcgcaggtat attagcagat ttaaaaatgg atccccatac agtggctaca ggcttcttac
    28441 atgatgttgt tgaagataca gaaatcactt tagaagatct gagagaagaa tttggcgatg
    28501 acgttgctat gttagtggac ggcgtaacca aattagggaa aataaaatat aaatcccacg
    28561 aagagcagct agcagaaaac caccgaaaga tgctgctagc aatggctcaa gatttacgag
    28621 ttatcatggt caaattagct gatagactac ataacatgcg tacgttgaag cacctgcgag
    28681 aagataagca aagaaggatc gctcaggaaa ctttagaaat ctatgcgcct cttgcacatc
    28741 gtctagggat cagccggatc aaatgggaat tagaagatac agcacttcgt tatctaaatc
    28801 caaaacagta ttaccgtatc gtccacttga tgcagacgaa gagagaagaa cgtgaaaaat
    28861 acgtaagtgg tactgttgaa gatattcgaa tagcgacaga agagctgggg atttttgcag
    28921 aaatctatgg acggccaaaa cacatttatt caatctatcg taaaatgaaa gatcagaaaa
    28981 agcagttcaa cgaaatttat gacttgctag ctattcgagt gatcgtagat tcaatcaagg
    29041 attgttatgc tgtattagga gcaatccaca caaaatggaa accaatgccc ggcagattta
    29101 aggattatat tgccatgcca aaagctaata tgtaccaatc tttgcatact accgtgatcg
    29161 gtccggcagg aaatccggta gaaattcaaa tccgaacaca ggaaatgcat gaaatcgctg
    29221 aattcggggt tgctgcacac tgggcctata aagaaggaaa aaatgaaaaa gtagaaccag
    29281 atggtatgac gaaacaatta agctggttcc atgagatact cgaacttcaa gacgaaagct
    29341 atgatgcttc tgaatttatg gaaggcgtaa aaggagatat ctttagtgat aaagtctacg
    29401 tcttcacacc aaaaggagac gttactgagt taccaaaagg atccggacca ttggactttg
    29461 catacagtat ccatacagat atcggtaaca aaaccactgg tgcaaaagta aatggcaaaa
    29521 tggtgcagct tgattacaaa ttgaaaaacg gagatatcat tgagatcatg acttctccaa
    29581 attcatttgg cccaagtcgc gactggttga aattagttgc tactagcaaa gcaagaaata
    29641 agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa tgtgatcaaa ggccacgaat
    29701 ccgtggtcaa atgtattaca gatctaggat ttacgcctaa agatattttg acgaagaaca
    29761 aactgcaaga agcactcgat cgttttaatt atcaaacaga agatgatctc tatgcagctg
    29821 tagggtatgg agaagttagc cccttgacga tggccaatcg tctgactgaa aaagaacgta
    29881 aagaacaaaa aatcgagcag caaaagcaag aagcagaaga aatcatgaat cagccgaaaa
    29941 aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt tgtcattcaa ggggtagaaa
    30001 acttactaat tcgtatcagt cgctgctgta atccgattcc tggtgatgat atcgttggtt
    30061 atatcactaa aggcagaggg atatccattc atcgtcgaga ttgtccgaat gttcagcctg
    30121 acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg ggaagataca tcgaatacac
    30181 gaaaagagta tgatgcagat ttggaaattt acggctataa tcgttcaggc ttattgaatg
    30241 atgtacttca aacagtcaat gcgctaacga aaaatctcaa cagcgttgaa gcacggacga
    30301 ataaagataa aatggcgacg atccatttga cggttggtat ccagaattta tcccacctaa
    30361 agagtatcgt ggataaaatc aaagcagtac ctgatgtcta cagtgtacgc cggacgaatg
    30421 gataggagga cagatatgag agcagttgtt caacgggtaa gtaaagctgc tgtgtcagtc
    30481 gatcagcaag aaattggaaa aattggctac ggcctacttg ttttattagg cattcatgaa
    30541 accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa ttgcacaaat gcgtattttt
    30601 gaagatgaac aaggaaaaat gaatattagt gtagaagatg tgggaggaca aattttaagt
    30661 atctctcaat tcacactgtt tgcggatacg aaaaaaggaa atcgaccaag ctttgtggaa
    30721 gccgcacgac cagaaacagc tattccttta tacgaatcat tcaatgaagg aatcagaagt
    30781 cgcggcatca cagtcgctac cggacaattc ggagcagata tgtcggtttc tctcgtcaat
    30841 gatggacctg taacgatcat tattgattct aaaaacaaat aaagtaatct ggttttcact
    30901 agtaaaaact atgaaacacg tcgcttaagt tagactgata aactctaact taagtggctt
    30961 ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa aaaaagttaa gcttaaatta
    31021 tacaagttat tttttatagg aggttgctga agatgaaaga agtaccagta gtagatatca
    31081 aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa catgttggag gagttacgcc
    31141 atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa taaagaagct atcacgatct
    31201 acggatcatc agcagcaaaa gtattttatg atccaaggaa tttcaaacga aaaggtgcta
    31261 tgccaaaatt agtattgaag acactttttg gtcaaggtgg agtacagact ttggatggag
    31321 ctgctcatca ccatcggaaa aatattttta tggatctaat gacaccggaa agaatggaag
    31381 attatcatcg tatcttagat aagaatctga cacaagcact tgaagcacag catggtcaat
    31441 ttgaactatt tgatttaagt aagatggttt tctttacatc tatttgcgag tgggcaggta
    31501 tcaatttatc agcgatttct aaagatgagg tcgaaaaatt agcagaatat caaatctcta
    31561 tgatcagcgg aacgtttact tctccgattg atcacataaa aggtgtcgag aatcgcaaaa
    31621 aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg acaaaatcct gtggctggaa
    31681 aagaaaatgt ggcattatat gcttttgcca atgccacaga tttagatggt cagcttttac
    31741 ctctggaagt ggcagctgtt gaattattga acatcatccg tccaacggtt gctctaactg
    31801 tgtgggcggc attgatggga catgcacttt tcagtagacc agatttgtat cagcaattaa
    31861 aaaatgactt ttcgacgtta caagatccgt ttattcaaga aatgagacgc tattatcctt
    31921 tcttcccaat gctacctgcg atttcgctga aagaagtaga agtagatggt tatcgtatac
    31981 cagaaggcag ttgggttatt ttagatttat atggaacaga ccatgatgaa cgaacagttg
    32041 aagcgccaga ttcctttatg atcaaacggt atgtagggaa agcaaaagat atttcctata
    32101 aagaagaata tgagatgatt gcacaaggcg ggggaaattt tcgacaaatg catcgctgtg
    32161 caggagaatg gatcactctt catagtctac gtgtgttttc cgatcagcta gtaaataagt
    32221 ttgagttctc tgtacctgaa caagattgga cgattccatt caatcagttt ccgacttatc
    32281 cgaatagccg agcgctactt tacaagaatt agattagcat aaacttatcg aaaaaaagga
    32341 agatcaaaca gtgctgatct tccttttttc atattactat aaattgataa ggtcaattga
    32401 tcaaaatcca cgaaatgctt catcaatggt ttgataatct tcattggata aatgcaaatc
    32461 taaagcttgg acattgtcag atacttgctc tggacgctta gctccaggta taactacaga
    32521 cactcgtgga ttcttcatgt accaagctaa aacaagttgc gcaggagtgg cttggtatct
    32581 tttagcaatg gggcgaagta catcgacagc ggttaaaatt tctctgaaac gttctccttg
    32641 gaaatctgga tttctttttc gaggatctcc ttcaccaaat tgtttttcct caccaagttc
    32701 atattttcct gtcagcaaac cggaagcaag aggaaaatag ggaacaaaac tgattttgtt
    32761 tttttcaaga taagggaata attctttttc agcctgccga tggatcaaac tgtatttatc
    32821 ttctactaca tcgacatatc catctgcatt cgcttctttc aattgttcta acgtgaagtt
    32881 tgacacaccg actgctcgga tttttcctgc ttcttttaat tcgtgcaatg tagcaactga
    32941 ttcatttttt ggtgtgcttt catccggaaa gtggatataa aaaatgtcga tgtaatcagt
    33001 ttgcagacgt tttagtgcat cttccaccga ttgtttgaga aactcaggag aattatcaaa
    33061 tgttcgccct ttatttggta catgagcagc ttttgtagcg atgacgacac gtgagcgatc
    33121 atattctttt tccttcagta cctcgccaat cagttcttcg gaacggccgt ttccgtaagc
    33181 ataagcggta tcgatcaaat tgatcccgct attcaaagct gtacgaacta cttgttttcc
    33241 tgtctcatct tccaaaccgt caaaaagatt atgtcctcca acggcatttg ctcctaatcc
    33301 aagctgttct gcgtagactt gtgaatgtcc gattcttatt tctgccatag ctgattcctc
    33361 ctttttgtct agtctaaaac aaaataccaa aaagctgaac taaaacgttt ttgtgagggg
    33421 aaatagtcag atatctcttg actttgagac tggtttttag taggttataa gtaatatcga
    33481 atgtcaatga aagagcgagt aagtttttga aactttacaa gagaggatca tcgaggctga
    33541 aaatgatcta aagtttcatt agcctaaaga cactctggag atggacgagt gaaaagccgt
    33601 cccgcgtacg agcgttattc gtaagaggaa agagaaatct ttcaaaatta ggtggtaccg
    33661 tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg tgattttttt atttacatag
    33721 ctaagaaagg gagagaaaac tatgagtttc caaagaccaa aaggaacaaa tgatattttg
    33781 ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag cacgtttatt attcagagat
    33841 tatcagtata atgaaatgcg tacaccgatt tttgaacact atgaagtgat atcccgaagt
    33901 gtaggggata caacggatat cgtttcaaaa gagatgtatg atttttatga caaaggggaa
    33961 cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg ttcgttcttt tgttgagcat
    34021 aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt tttatatggg accaatgttt
    34081 cgctacgaac gccctcaaaa aggacggtta cgtcaattcc atcaaatcgg agtagaagca
    34141 tttggatcag aaaaccctgc aactgatgtg gagagcatgg tgatggcact tgatttcttc
    34201 aaacagttgg ggatcacgca aatccggcta gtcatcaatt cattaggtga taaagaaacg
    34261 cgtcaagcct atcgtcaagc attgatcgac tatcttttac cttttgaatc agagctgagt
    34321 gaagactcta aacgccgcct tcatgaaaat ccattacgcg tattggacag taaagacaaa
    34381 cgagatcaaa aatttgtggc agaagctcca tctatcttag attatctaag cgagcctgct
    34441 caaaaacatt ttgataccgt tgtttccatg ctggatgcat tggctgttcc ttatgaaatc
    34501 gacagtaata tggttcgcgg actagattat tacacacata cgatttttga aatcatgagt
    34561 gacgcaccaa aaatgggggc acaagcaacg atttgcgcag gcggacgtta tgacaatcta
    34621 gtggaagaac taggtggtcc ttcaacacct ggcttcggat ttgcaatggg tatcgagcga
    34681 ttactcttga ctatggaggc agaggaagta gttatccctg catttaatga gttggatgct
    34741 tacgtggtcg cactcggaga cgaaacaaat atcgaagcat tgaaagtggt ccaagcgatt
    34801 cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc gtaaagcaaa agcgcaattt
    34861 aagacagcag ataaagcgaa tgccaaatta gtattggtga tcggtgaaga tgagcttgct
    34921 agtcaaacga ttaatgtcaa agcaatggca agccgaaaag aaaaaagttt tccattagaa
    34981 gagatatatg aacattttga tgaagtctat gatgaaatga ctttattcga gtaggaggaa
    35041 aaaaataaat ggcacaaaga acgatctatt gcggattagt ttccgaacaa caattagaac
    35101 aaacagttac attaaaagga tgggtacaaa aaagacgaga ccttggggga gtaatcttta
    35161 tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt taatcctgaa atcaacaaag
    35221 aagcatggga aatcgcggat aaatgtcgca gtgaatacgt gatcgaagtg actggggaag
    35281 ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc cactggtgaa ttcgaagtga
    35341 ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc gccatttcaa attgaagatg
    35401 atcaagcaat cagtgacgaa atacgtttga aatatcgtta tttggatctg cgtcgtccaa
    35461 aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa agcgattcgt cattatttag
    35521 atgataatga ctttctcgat atcgaaacac cttacctagg gaaatctaca ccagaaggcg
    35581 caagagacta cctagttcct tcacgtgtcc atgctggaca tttctatgcg ttgccgcaat
    35641 ctcctcaatt gtttaaacaa ttgctgatga atgctggatt cgaccgttat tatcaaatcg
    35701 ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca gcctgaattt acgcaagtag
    35761 atattgaaac aacgttctta acagcagaag aaatccaaac ttacacagag ggattgattg
    35821 cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt gccatttcct cgtatgacct
    35881 atgatgaagc aatggctcgc tacggcagcg acaaaccgga tacacgtttt gatatggaat
    35941 tgatcgatct aagcgataca gtcaaagaag tagaattcaa agtcttccaa atggctcttg
    36001 aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc tgcagatcgc tactcacgaa
    36061 aagacatgga tcagttaggt caatatgtag gacaattcgg agctaagggg cttgcatggc
    36121 tgaaagtaga agaagacgga ttgaaaggtc caattgctaa attcatggga gaagcaacag
    36181 aagcaatcat taaagcaaca gatgcaaaac caggtgactt gctgatgttt ggcgcagata
    36241 aaagtgaaat cgtcgctgct gcattaggtg ctatccggac acgtttagga aaagaattag
    36301 gactaattga tgaatcgaaa ttcaatttct tatgggtaac tgattggcca caatttgaat
    36361 tttcagaaga agaaggtcga tatgtctctg ctcaccatcc attcacgatg ccaaaagaag
    36421 aagatatccc gttattggct gaagaccctg caaaagtcta tgcagaagct tatgatatcg
    36481 tcttgaacgg ttatgaatta ggcggaggtt ctatccgtat tcatactcgt gagcttcaag
    36541 aaaaagtact agaaactcta ggctttacaa aagaacaaat ggaagaacaa tttggtttct
    36601 tgttaacagc tttagattac ggattccctc cacatggcgg tattgcatta ggattggacc
    36661 gttttgcaat gttattagcc ggagaagata atattcgtga agtcattgcc tttcctaaaa
    36721 acggaaaagc agcagatgtg atgagtgatg caccaagtat ggtttcgcct ttacaattgt
    36781 tcgaattgaa tattgatgta acgaatatag aagaatagcc gataaaaagg taaaaaggat
    36841 ttgtcgaata cgcttctttt gacaaccttg atttaattga aaaacacttt ataatgaagc
    36901 cataacatat aaagcggggc ttttccatga aaaaaacatt cgaaaattgg gcttatcatg
    36961 attacacgac caagctttcg gtatctatcg tttatgcgtt gatcgcatca gtcgcattga
    37021 atttctttta tcagccagga catatttatt caagtggtat tactggtcta gctcagattt
    37081 tgacgacttt gtcaaaatca ctgataggat tcgaagtacc ggtatcaaca actttgtatt
    37141 tactgaatat acctttgttt gtattggcgt ggttaaaaat cgggaagaag tttacaattt
    37201 tcacttttat cacagtcact ttgacctccc tttgtatcca tattttaccg gaaacagtcc
    37261 tatctaccga tccaatcatc tgtgcgttgt ttgggggggc tgtgatggga ctaggtattg
    37321 ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga tatcgtgagt atcacgattc
    37381 gtaaaaaaac aggaaagtcg attggttcta tttcgatcta cttcaatgca ttgatcgttt
    37441 ttgttgccgg aattctattt ggctggcaat atatgtttta cagtacgttg tctattttcg
    37501 tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa gaagatgcag gtaatgatcg
    37561 taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa ctgtatgcgt agagggatca
    37621 cgatcatcaa tgaagcagaa ggtgcttata agcatgataa gcagacagta ttgatgacga
    37681 tcgttacacg atacgaactg cctctactta gagaagcaat gaaagcttct gatccacaag
    37741 cttttgtaag tattgcagaa aacgtccaga tacttggtcg tttctatgag gatggcattt
    37801 agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga cagacgttcg tttgtctgca
    37861 gtttttttct accatagtag aatcagcaaa gaaaaactgc ttttttattt gaaattttct
    37921 taaagttgaa aaaaagcatg tttatttttt taaatttatg ggatgataga aaaaaatagt
    37981 agagaaagga cacgattcta cttgctattt tcacttaata cccgtatatt acgaatgaaa
    38041 aaagtttatg aaaagttgga atattgaatg gaaaaacaaa aaaggttaaa aaacagccgc
    38101 tacatcactg ggtttgatgg gctccgttca ttagctgtgg tgggtgttat cttctatcac
    38161 cttctgccaa ctagtatgaa aggcggctat ttgggcgttc cgattttttt cgtcgtatct
    38221 ggttatttga tcacggatct gctgcggcag gaatgggaac aaaatggaaa aatcaatatt
    38281 tggcaatttt atgtcagaag aatgaagaga ctctatccgg cacttgtttt tatgcttgtg
    38341 acagcttcgg cttatatcac tttgtttcag cgagggctgt taaacaatct tcgaggagtt
    38401 gtggtcagca gcttactgta tgtcaacaac tggtggcaaa tcaataacgg tttatcttat
    38461 ttcgatcgat ttgctaatga gtcaccgttt acgcatatct ggtctttagc agtagaaggt
    38521 caaaactatt tggtttggcc gatcttgttt gtattgttga tggtatttgt tcgtaaaaga
    38581 aaatggatct tttatactgt gttaggtaca tccttcctct ccgccatctt gatgatgatc
    38641 ttgtatacac caggaggcga tccaacccgt gtttattacg gtaccgacac tcgtctgttt
    38701 tctatatggt tgggaagtgc gcttgcgttt gtttggccaa gtacgcgttt aaagaaaaac
    38761 atcccgaaac aagcgaaaaa tattctgaat atcgctggag gaatctcgct tttggcactg
    38821 attctttgct tcttcttttt agatgatcat tacaatttca tctattacgg aggaatgctc
    38881 ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag cacaccccgg agcaagtttg
    38941 aatcgatggc tgacaaatcc agtatttact tggattggca aacgaagcta tggcatttat
    39001 ctgtatcagt ttccagttat gattttttat gaagcaaagg tgactgatat tgccgatcat
    39061 gttttgttgc atactttagt ggaaatcaca ttgattctaa tcatcagtga actttcttat
    39121 cgatatgtgg agcgtccgct agcacgtttt gattatggtc gtacattcca agtgatgaaa
    39181 ggatggttca gcaaaccgat catcagtaag agcaaaccat gggttgttcc aggcacatta
    39241 cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga aaaacgctgt aacagccgat
    39301 caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat tagcagaaga aaccagaaac
    39361 aattcagaaa acaccactag ttcggttgac caagcgatcc ttgataagta cgagttgtcc
    39421 aaggcacaag ggaataaggc acaaaaatta gaactaacgg catttggtga ttcagttatg
    39481 ttggatgctg caacggatct acaagaactt tatccaaaag tagtggtaga tggagatgtc
    39541 ggacgacaat tatatgccag tgtaccgtac attgaaaaat taaaggaaca agatttgttg
    39601 aaagacactg ttctgattgg actaggaacg aatggctcat tctcggaagc tcagtttgat
    39661 agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg tcaatgtccg agtaccgacg
    39721 aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga tgacgaaaaa atacgacaat
    39781 atgacgctga ttgattggta cagctacagt aatgaccata gtgactggtt ttatgatgat
    39841 caagtccatc caaatcctga agggatgaca cattacattc acttagtcag cgaagccttg
    39901 ctaggaggaa gttcaagtac gaacagcagt gagacaaacg tgacagaatg atagctacaa
    39961 aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt tttatattta ccgtgttttt
    40021 aacattcata tagattcagg aagcaaaacg gttgagtgtg tctgtaaata acgttctttt
    40081 tgttttcggg tgagtttttt gaatgcagct tctgcttttg ttgcttcgct tcttgaagaa
    40141 aatacttctg catggatcat cttaacaggt cttctttttt gaagacgagt atatttagca
    40201 ccagtaccat tgttatgttc ctctaatcgt ctagaaagat cggttgtata tccaccataa
    40261 aatgtctgat ctttacagaa taatacgtaa aaataatgtt catttgccat agagcatctc
    40321 acgaatctct gcagtgtatt ctccatccat attgtaagtg atcaatggtg gagcgacttt
    40381 gaatccgtct aatttgcctt ctttgattcc ttcaactaac aaaatatttg cctcttttcc
    40441 aggttttggg taaacaaatt ggatcctttt aggtgcaatt cgatagtctt ccatgacctg
    40501 aatgatttct aagaaccgat caggacgatg gaccatagta aagcgtccat ttgtttttag
    40561 tagcttactt gaaatatcga tcacttcttc taacgtcgta tgaatctcat gacgggcgat
    40621 tgccaggtgt ttattagggt tcgttttatt tgttggcagc cctttgaaat aaggtggatt
    40681 acagacgact aagtcacaag aattatgtcg aacgacagat aagctgtctt tcagatcgat
    40741 ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg atactacgtc ttgccatgtc
    40801 tgctagacga ggttgaagtt cgatagcgtc gatagccgcc tgggttcgtt tacttaaaaa
    40861 aagtccaact gcgccatttc ctgcacaaag gtcaacaatc ttcccttttt taggtacacg
    40921 ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa aacactttgg aactttggat
    40981 aatcttgata tcttctgcgt ataattggtc gattcgttcg tcttcgtgaa gcatggattc
    41041 cctccaaaat gaaaaaaata gtcgtctgta ttatactatt tttattaaaa agaaccaagg
    41101 tggcttgtca aacctctatt ttttatgcat actatgacca taagaaggga aaggaagaga
    41161 ctatgtttta tcgatttatg cgcggggtag ttcgagtact attagcctta gttaatggaa
    41221 atgcccgcta tgagaataaa gaagttttac cgcaagatga gaattacata ctagttgctc
    41281 cccatcgtac ttggtgggac ccattatatc tggcggtagc tgcagcaccc aaggaattca
    41341 gctttatggc aaaagaagaa ctgttcaaaa atccagttct gcgatttata ttaactcgat
    41401 ccaatgcttt tccagttaaa agagaaaatc caggaccaag cgtcattaaa acacctgtaa
    41461 agattttgaa atcgacaaat ttgagtttga tcatgtttcc cagtgggaca agacatgcta
    41521 cagaacttaa aggcggaatg gcattgattt ccagaatgtc taaagcgaaa atcgtgccag
    41581 ctgtatatca aggtccgttg acacttgggg atctgttcaa gcgtcagaga gtgacagtgc
    41641 gtttcggcga accaatcgac ttatcagata tcaagaaaac ggataaagaa ggtatggcgg
    41701 agatcgaacg acgaacacaa gcagcttttg acaaattgga caaagaagtg aaccctgatt
    41761 tcaaatatga agtcaaaaaa aaataaaatg ataagtttat gcgtgggcgg tcttttgaat
    41821 ctactgtcct gttcagttca gactttttca atgaaaacga gggagagacg tcaagtctgc
    41881 cctctttttg cgttccaata tgtaaaatag aagacaggtg tcatgtaatg gatcaaagtg
    41941 ttataataat tagaaggaca ttttttgagg tgattgaatg cgttttttac atacagccga
    42001 ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt gaagaccagc gtcatgtact
    42061 atcttctata ctagatatcg cgaaagaaga aaaagtagag gcaatcgtga ttgccggaga
    42121 cttatatgat aggagtgtcc cagctgttga tgccgtagaa ttatacaatg aattgatggt
    42181 cgattggaat ttaaaggaaa agatcccgat ttttgctata tcaggaaatc acgatagcag
    42241 tacgagactt tcagcgggta cgccatggtt cagttattcg aactattttt tgcgaacaca
    42301 gctagcagaa gcatttgaac cagtcgagtt taaaaacgta caatttttct ttcttcctta
    42361 ctttgaacca attgcagcga gattatattt tgaagatgac aatatccgaa cgatcaagca
    42421 agcggtagcc aaggtgatag ataaaatgca ggagctattt gattcagaaa aaaaacaagt
    42481 attgattagc catttttttg taatgggaag tgtaaaatct gattcggaaa cgaaaatcga
    42541 agtaggcggg ttggattcga tccctggtga actacttaca ccgtttgatt atgtagcact
    42601 aggacatttg cataatcaag ctgctttgag acaggataat gctcgctata gcggttcccc
    42661 attgaaattc tctttatctg agatcaacca aaaaaaaggc gtatggatcg ttgacctgac
    42721 aacaggcctg gagttgaatt ttagggagat cgaacccctg catgatattg aagaaataac
    42781 agcgagtttc aaagaattgc tggatcctgc cttttatcag tccgtcaatc gggagaatta
    42841 catctatatc tatctcactg atagaacgat catcccgaat atgatgaacc agcttcggca
    42901 aatatatcct cgtattttgg ggattgaacg ggcaaacggt cgagcagaac aaacaaagcg
    42961 caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta gcagaagcat tttttagtga
    43021 aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg ctcggagaaa cgttgcaaga
    43081 aatcatggaa atggaatgat caagaatgaa accgaaaaaa ttacaactta aaaattttgg
    43141 tccttttatc aatgagaccg tagatttttc acgtttgaca gaagcaccgc tttttttgat
    43201 cagcgggaag acaggagctg gcaaaacgac gatttttgat gggatcacct tcgcactttt
    43261 tggtgaaact tccggaagac ttcgctctgg aaaagagatg cgttctttgt ttgctacacc
    43321 agacgaagaa acaagcgtga ctttttcttt tgaacatcag cagatgagct atgaaatcga
    43381 acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac ggaacaaaaa aacaggcagc
    43441 caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa ataaaacaaa tcacaaaacg
    43501 tacagaagca gatcagttga tcagagaact gatgaatctc gatgcgaaac agttttctca
    43561 aatcgtttta ctaccccaag gagaattccg taattttttg gtttcatcaa gcagtgataa
    43621 agagactgtt ttacgtcatt tattcggtac tcaatttttc caacagttca atgagcgatt
    43681 aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat ttagagcaag agctgcatct
    43741 tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca gcagcagggt ttgaagccgt
    43801 tttagcccaa tggacaaacc atcaggcaat actcgaaaaa caaatcacgg aagaaaaaga
    43861 agctctaacg gaacttcagg aaaaacaaaa gcaactagaa aattcctact atcgttttga
    43921 aagcttgaaa aaaagttatg aagaaaaaac gatattaata aacaagcaaa aggaattagc
    43981 tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt gggtattatg aatttacgca
    44041 acgattagtc gaaccaatcc agcgcctaca agaaataaaa gttgaaaaaa tagaactgtc
    44101 acaaaaaagt agtgagcaaa caaagaaact agcgatgact acgcagaatt atgaagagtg
    44161 gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa atcaaagaag ctgctcaaaa
    44221 actgcaaaac aaacagatga tggtgccaat tgtcgaatcc ttacgtagta aaaaacaaga
    44281 cgcagaacaa ttaacaattt cattggataa agaagaattg aatgccgaga agctgtatca
    44341 aaatctcgct atctatcagg aaagaactcg aattcttcaa gaaaatttac tggatcagga
    44401 aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa tatctagatg aaaagcagca
    44461 agaaatagaa aaacaaaaag caaaagacac tgggtcgctc gcacagataa gagaagaact
    44521 caaaaaagca actgaagaaa taacaaaaaa tagtcagcta ttgaaagaac agacagaaca
    44581 agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt gctcgtcttc agcttttttt
    44641 gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa caccccatac aacaattaaa
    44701 acacgaacag ccaaataagg aagatatctt aaaagtagaa caacaactgg cagaagctga
    44761 gtctgctgta gaaagaactc agcaaatcat tgccaaaagt gaagcacatg aaaataatct
    44821 tcaacaacaa ttatcagatt tagaacaaaa aatcatggca acgaatatac aattggctga
    44881 acaagaagaa cacttgcaac aggctttgaa aaaggcattt cctgagataa cagaacaaaa
    44941 aacaaaaaca actcttgtag taatcgaaca atctttagat agacgaaaaa gcaagatggc
    45001 caatgaaaga atagagctgg aagaaataag acaacaagca gaaaagctcg aacaacaatt
    45061 agctgtctac aaacagcagc agacagaaaa aaataatcag ctccagcaac tgcaaggaga
    45121 aatcaaaagt ttagaacaac aattgcaaga tacggatagt aactgtttag aagaggagat
    45181 acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa ctagctgttg agcagaaagc
    45241 aggagtgcaa ttgttgcaag atcttcatac gcttcggaca cagcttgatc ttcaacatga
    45301 acaaatagaa aaaatagatg aaaaaataga aaaggaacaa gaaagaatag ctaaaaagtt
    45361 agaaagccag tcgtattttt caacaattga agaaatctgt gcatttgcac aacagcaagc
    45421 acaatataaa gaaaaccaac gtacagttac ttcttatcat gaagagcaat tagtgacagc
    45481 tgaccggctg cgtcaattag aaaatatcca gctaactgag acattcccag atacagaaga
    45541 gatcagccaa cagttagaag aaataagacg tatgatcgaa ataaagcaag aaaagatata
    45601 cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac gatgaatgtc tttcgattta
    45661 tcacacaagc cagacgaaat tagatgagct tacgcaatta cagcagcttt cgcaaacatt
    45721 caacggagaa aatcctaaga agactagttt ggaacggtat gtgcttcagg tttatcttca
    45781 agaagtgtta caagtagcaa atgatcatct ccaacgttta acgaaaaatc gttatcaatt
    45841 tgagctagcg gataccattg gcagttatcg agggaaaacc ggattagaaa tcaatatcta
    45901 tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg tctggcggtg agagttttat
    45961 tgcagcactt tctttagcat tagctcttgc cgatgtgatc cagacacaag ctggtggtgt
    46021 agcaatcgat gcattgttta ttgatgaagg atttgggtca ttagatgaag aagctttgga
    46081 aatggcaatg gaagctttag aaacgatcga aagtgaagga agaatgatcg gcatcattag
    46141 tcatgtaaga gaattgaaag aacgagtatt acaacaaata agaattgata cagaaggtag
    46201 cggacaaagc aaaatacgct atcaacttgg ataaaaaggg agattggtaa ctatgaaatg
    46261 gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac ttgcaagaag gccgagtgtt
    46321 atcggttgtc cctgaccaag aagaaaacgt gtggcatgca gaagtcttgg gcagtgaatt
    46381 gtatatggtt gatttggatg gtacagcaaa agagatagat tactgccaat gtccctattg
    46441 ggaggaacat aaatattgta agcatacagt tgctgtggaa ctttatttac ggtcgaaaaa
    46501 tctatctcgt atcatgaaaa aagaccagcc aatgctagaa ccggtaaaag caacaagtga
    46561 gggagaaatc attgcaaaag gtttttctcg tctaaaaaca caaaacaatc ctcaagcggt
    46621 gcaacctttg attattgaat accaagtaga aacgattgag actaatcagt atcacccaga
    46681 gctttctatt ttggcacttt acttgcgtat cggttcattg gcatcaccga aaaaaacata
    46741 tatcgtaaaa aacatctatg attttttaca agcttacaca gaaaaagaaa cttacactgt
    46801 taataaacag taccagtttc gtctagataa acgggcgttt caaaaagaag atcaaaaaat
    46861 actcacgcat cttgcaggaa gtgctcagac gcatcaatta ttaggtacga acggcttaca
    46921 agtcaaagga aaactagata aaagatatct gcttctccca gtagaacaag cccgcttcct
    46981 gttagagcag atgaatcaga ctacacgttt gcatctgcag ataaatgaac aaaacgatca
    47041 aggaatacaa ttttctgatc aaaacaaacc tttatcattc aaagtggaaa aatttggaga
    47101 agattatttg ctgcaagcat tgaatgattt tgattttttc tttatgcatt atcagtgggg
    47161 agttatccaa gggacatttt attctctgac gaaatatcaa caaacggttt atcaaacttg
    47221 gaaacaattg attcgccgat tggagaaacc agaagtagtg ttcaagaaaa aagaattacc
    47281 gtctttgttt aaagagatta tccctctgct gtcagagatc ggcgacgtag ctattgcccc
    47341 tgcagtagaa gaggaagtag cagatatccc cgtcgaattt attttctttt tccgaaaagc
    47401 aaaaggaaaa atccaagctc gagtcgattt cgtctatggt gaagtcatct attcgactga
    47461 cccgaagcat gaggtctcga cagaacaggc ttttcaggtg ttacgtgaca cagtagaaga
    47521 gaaacgagta cttgatctat tcagaatgta tcgctaccag aaaaatgaaa ctggttatga
    47581 acgtccattg ccaagcgggg aagaactata tgcctttttc cgcacagaac tatctgtctt
    47641 ccgtcagttt ggtgaagtca gacttggaag aaaattgcga gagttgtatt tagatgcgca
    47701 taaacatcaa ccgaaaatcg aagtaaccga gaccggttca tggctcgata ttcgattcga
    47761 tgtgacaggg atagaggagc aagaaatcga tcatgtcctg caaagcttgc tgagaaatga
    47821 tgctttttat acattagaaa atggtcaggt tctttctttt gattcagaag aatttcaaca
    47881 aacaagccaa gtgttgcaac aattacggga atcgatacgt acagaagaag gaacgatcca
    47941 tgtacctaaa aatcaaggat tgatcattca aaatcaattg gaaaaaagca atgctacttt
    48001 tagtgaatcg ttccaaacga tggtacaaga tctcattcat ccagaaagat accaggcaca
    48061 gcttcccaaa ggattgaatg caacgatgag agattatcaa aagcaaggtt ttcgatggtt
    48121 gaagatgtta ggtcattatc agtttggcgg tattttagcc gatgaaatgg gacttgggaa
    48181 aacactacag acaattgcct ttttactttc ggaaaaagaa gaacggaaaa gcttctctgc
    48241 tttgatcgta gcgcctgcga gcttaattta caactggcag gcagaggtga gaaaattcgc
    48301 cccttcttta agtatccaag taatcaatgg gaataaaaaa gaacgtgaag aattactggc
    48361 taaagacaca gatatccgag tgacttctta tgccagctta agacaggatc tagctaatta
    48421 tcagtcgcaa aaaattgatt atttgattct tgatgaggca caaatggtca aaaatagcag
    48481 tacgaaaact gcacaagcat tgagagaatt agctgtacct cagcggtttg cactgagtgg
    48541 gacaccaatc gagaataatt tagaagaatt gtggtcattg tttgcaacga ttatgccagg
    48601 atttttcccg actaaaacaa aattcaggga acttgccgca gaagaaattg cgcaaatgat
    48661 tcgaccgttt attttacggc gagataaaca aactgtgttg aaagacttgc cggaaaaaac
    48721 agagatgaat ctatattctg cacttacaga agaacaaaaa actgtttatt tagcttactt
    48781 gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa gcattcaaga aaaaccggat
    48841 cgggatactt gctggtctga caagactgcg acaaatttgc tgcgatcctc gtctgtttat
    48901 tgaagattac caaggcggtt ccggcaagct cgaacaagta aaagatctac tggtagctgc
    48961 caaagagaac aaacgaagag ttttgctgtt ttctcaattt acagggatgc tgactatctt
    49021 gcaagaagag ttagccgagc tagggatctc gacattttat ttaagaggca gtacgaaacc
    49081 acaagatcgg ttaagtatgg tagacgcatt taatgctggc gaaaaagatg tttttctgat
    49141 ttccttaaag gctgggggaa ctggattgaa tctcacggga gcagatacgg tcatcttgta
    49201 tgacctttgg tggaatcctg ccattgaaga acaagctgct ggacgtgcac atcggatcgg
    49261 acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa ggaacgattg aggaacgcat
    49321 ggattcactc cagcaagaaa aacgcgaact gttccaaaaa gtgatccaag gtaatgaaga
    49381 acagttgacg aaactgactg aagaagacat ccgcatgatt ttaagtgtag gagaaatcga
    49441 ggagtaagag actcgaaaaa gcgttcagta atgggtaaga aagcctttct aaagaagcga
    49501 ttgtttattt gatttaaaaa atagcaagga ggctgggatt ccattttatc ccagcttcct
    49561 tgctatcatt tttttatttc tatttttaaa ggttatacaa agattgtctt cattgtctct
    49621 aacagctgat caaaagaaga aatctcgata tcgcaaattg gagtctgttc aattttgcgt
    49681 tctcgatggt tgaaccaaag cgcttgccag ttagcacttt ttgcacctag aacatcattg
    49741 tcgtaattat cgccaacgta taaggtctct tctggaagca tatgaaattc tttctcagcc
    49801 agctgaaaaa tctctttttc tggtttttgg aaacctgttg cttgagaaat gagcatattg
    49861 cgggaaggaa tccaattgtt caattgaagt tgcttcagtt tttttgtctg atggtcagtc
    49921 ggcccatttg tgataattcc taatgggaca ttcttttcct tcaagaaatc gagggttttt
    49981 ttgacctcct catgcaagca gatattatca agttcttcct cgtaaatttt ttgaaataag
    50041 aggccatctt cttccgtaat atgagggtag tctaaatctt ttagtgattg gctgatccga
    50101 tgtgctcgca tatattctag tgtccaatct cctgccatta cttttgggaa gttttcatca
    50161 ctatgatggc ggaaccggat atatagtgga tgcatatctg catctgaaac aagcggaacg
    50221 actcttttga ccgcgtttcg aaatggctgc tgctgatcat acatcgtatc atcaacatca
    50281 aacacaactg atttaatcaa aattttacac gcctttcggt tttcattgat tttttcacaa
    50341 atccttgact attgtaacag aaaaaattgt ctaaaaaagc tgataaataa aggtttttct
    50401 tactatattt ctatttcctt gacagaataa ggtttttata tttcagtttt tttcactaaa
    50461 aatcgtgaaa cacgtcaata agtttgatga acaaattaat cattatggta gaagtcgatt
    50521 gatttatttt aatgcaatct ctgtggaatt ttcgaaagat ttattttttt aatacagtct
    50581 caatataact attcaaaca
//
LOCUS       DAYYOA010000008        47020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000008 DAYYOA010000000
VERSION     DAYYOA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.008"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACHBKA_000439"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACHBKA_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM9444157.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACHBKA_000440"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACHBKA_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM9444158.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHBKA_000441"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHBKA_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM9444159.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHBKA_000442"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHBKA_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM9444160.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACHBKA_000443"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACHBKA_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9444161.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACHBKA_000444"
     CDS             7565..8422
                     /locus_tag="ACHBKA_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM9444162.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACHBKA_000445"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACHBKA_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM9444163.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACHBKA_000446"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACHBKA_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM9444164.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACHBKA_000447"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACHBKA_000447"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9444165.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACHBKA_000448"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACHBKA_000448"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9444166.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACHBKA_000449"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACHBKA_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM9444167.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACHBKA_000450"
     CDS             13203..13895
                     /locus_tag="ACHBKA_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9444168.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACHBKA_000451"
     CDS             13892..14659
                     /locus_tag="ACHBKA_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9444169.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACHBKA_000452"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACHBKA_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM9444170.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACHBKA_000453"
     CDS             16624..16944
                     /locus_tag="ACHBKA_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM9444171.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACHBKA_000454"
     CDS             16944..17294
                     /locus_tag="ACHBKA_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM9444172.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACHBKA_000455"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACHBKA_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM9444173.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACHBKA_000456"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACHBKA_000456"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM9444174.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACHBKA_000457"
     CDS             19288..20313
                     /locus_tag="ACHBKA_000457"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9444175.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACHBKA_000458"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACHBKA_000458"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9444176.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACHBKA_000459"
     CDS             21391..21831
                     /locus_tag="ACHBKA_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM9444177.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACHBKA_000460"
     CDS             21834..22436
                     /locus_tag="ACHBKA_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9444178.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACHBKA_000461"
     CDS             complement(22495..23601)
                     /locus_tag="ACHBKA_000461"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM9444179.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHBKA_000462"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHBKA_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM9444180.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="ACHBKA_000463"
     CDS             complement(25149..27494)
                     /locus_tag="ACHBKA_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM9444181.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="ACHBKA_000464"
     CDS             27773..28675
                     /locus_tag="ACHBKA_000464"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM9444182.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="ACHBKA_000465"
     CDS             28888..30663
                     /locus_tag="ACHBKA_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM9444183.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="ACHBKA_000466"
     CDS             complement(30729..32315)
                     /locus_tag="ACHBKA_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM9444184.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="ACHBKA_000467"
     CDS             32566..32790
                     /locus_tag="ACHBKA_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM9444185.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="ACHBKA_000468"
     CDS             32932..34683
                     /locus_tag="ACHBKA_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM9444186.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="ACHBKA_000469"
     CDS             34683..36467
                     /locus_tag="ACHBKA_000469"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444187.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /gene="licT"
                     /locus_tag="ACHBKA_000470"
     CDS             36793..37644
                     /gene="licT"
                     /locus_tag="ACHBKA_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM9444188.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37737..39623
                     /locus_tag="ACHBKA_000471"
                     /pseudo
     CDS             37737..39623
                     /locus_tag="ACHBKA_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(39663..40565)
                     /locus_tag="ACHBKA_000472"
     CDS             complement(39663..40565)
                     /locus_tag="ACHBKA_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9444189.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="ACHBKA_000473"
     CDS             40796..42199
                     /locus_tag="ACHBKA_000473"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9444190.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="ACHBKA_000474"
     CDS             42199..43632
                     /locus_tag="ACHBKA_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9444191.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="ACHBKA_000475"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="ACHBKA_000475"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM9444192.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="ACHBKA_000476"
     CDS             44917..46644
                     /locus_tag="ACHBKA_000476"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM9444193.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="ACHBKA_000477"
     CDS             46659..47009
                     /locus_tag="ACHBKA_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM9444194.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        15195 a         8272 c        10123 g        13430 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa
//
LOCUS       DAYYOA010000009        45514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000009 DAYYOA010000000
VERSION     DAYYOA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.009"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHBKA_000478"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHBKA_000478"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM9444195.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHBKA_000479"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHBKA_000479"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM9444196.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHBKA_000480"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHBKA_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM9444197.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHBKA_000481"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHBKA_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM9444198.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHBKA_000482"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHBKA_000482"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM9444199.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHBKA_000483"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHBKA_000483"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM9444200.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHBKA_000484"
     CDS             complement(8053..8523)
                     /locus_tag="ACHBKA_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM9444201.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHBKA_000485"
     CDS             complement(8569..8994)
                     /locus_tag="ACHBKA_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM9444202.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHBKA_000486"
     CDS             complement(9061..9330)
                     /locus_tag="ACHBKA_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM9444203.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHBKA_000487"
     CDS             complement(9341..10936)
                     /locus_tag="ACHBKA_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9444204.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHBKA_000488"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHBKA_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM9444205.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHBKA_000489"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHBKA_000489"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM9444206.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHBKA_000490"
     CDS             15402..16376
                     /locus_tag="ACHBKA_000490"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9444207.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHBKA_000491"
     CDS             16546..17946
                     /locus_tag="ACHBKA_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM9444208.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHBKA_000492"
     CDS             complement(18024..18746)
                     /locus_tag="ACHBKA_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9444209.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHBKA_000493"
     CDS             complement(18825..19331)
                     /locus_tag="ACHBKA_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9444210.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHBKA_000494"
     CDS             19454..21253
                     /locus_tag="ACHBKA_000494"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM9444211.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACHBKA_000495"
     CDS             complement(21321..22895)
                     /locus_tag="ACHBKA_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM9444212.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHBKA_000496"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHBKA_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM9444213.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHBKA_000497"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHBKA_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM9444214.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACHBKA_000498"
     CDS             complement(24672..25310)
                     /locus_tag="ACHBKA_000498"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM9444215.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHBKA_000499"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHBKA_000499"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM9444216.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHBKA_000500"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHBKA_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM9444217.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHBKA_000501"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHBKA_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM9444218.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACHBKA_000502"
     CDS             complement(29104..29844)
                     /locus_tag="ACHBKA_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM9444219.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHBKA_000503"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHBKA_000503"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM9444220.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHBKA_000504"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHBKA_000504"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM9444221.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHBKA_000505"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHBKA_000505"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9444222.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHBKA_000506"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHBKA_000506"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM9444223.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHBKA_000507"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHBKA_000507"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9444224.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHBKA_000508"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHBKA_000508"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9444225.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ACHBKA_000509"
     CDS             complement(36724..37008)
                     /locus_tag="ACHBKA_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444226.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ACHBKA_000510"
     CDS             complement(37026..37916)
                     /locus_tag="ACHBKA_000510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM9444227.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ACHBKA_000511"
     CDS             complement(37936..38154)
                     /locus_tag="ACHBKA_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM9444228.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ACHBKA_000512"
     CDS             complement(38124..39824)
                     /locus_tag="ACHBKA_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM9444229.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ACHBKA_000513"
     CDS             40091..40864
                     /locus_tag="ACHBKA_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444230.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACHBKA_000514"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACHBKA_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM9444231.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ACHBKA_000515"
     CDS             complement(41688..42659)
                     /locus_tag="ACHBKA_000515"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9444232.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACHBKA_000516"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACHBKA_000516"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM9444233.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACHBKA_000517"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACHBKA_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM9444234.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT        12397 a         9875 c         8236 g        15006 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgag
//
LOCUS       DAYYOA010000010        43754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000010 DAYYOA010000000
VERSION     DAYYOA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.010"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(394..2775)
                     /locus_tag="ACHBKA_000518"
     CDS             complement(394..2775)
                     /locus_tag="ACHBKA_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9444235.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2765..3412)
                     /gene="recU"
                     /locus_tag="ACHBKA_000519"
     CDS             complement(2765..3412)
                     /gene="recU"
                     /locus_tag="ACHBKA_000519"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM9444236.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3476..4015
                     /locus_tag="ACHBKA_000520"
     CDS             3476..4015
                     /locus_tag="ACHBKA_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM9444237.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4105..4524
                     /gene="gpsB"
                     /locus_tag="ACHBKA_000521"
     CDS             4105..4524
                     /gene="gpsB"
                     /locus_tag="ACHBKA_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM9444238.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4546..4913
                     /gene="rnpB"
                     /locus_tag="ACHBKA_000522"
     ncRNA           4546..4913
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHBKA_000522"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5122..6288
                     /locus_tag="ACHBKA_000523"
     CDS             5122..6288
                     /locus_tag="ACHBKA_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM9444239.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6334..7830
                     /locus_tag="ACHBKA_000524"
     CDS             6334..7830
                     /locus_tag="ACHBKA_000524"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM9444240.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7911..8594
                     /locus_tag="ACHBKA_000525"
     CDS             7911..8594
                     /locus_tag="ACHBKA_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9444241.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9239..10429
                     /locus_tag="ACHBKA_000526"
     CDS             9239..10429
                     /locus_tag="ACHBKA_000526"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM9444242.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10531..11484
                     /locus_tag="ACHBKA_000527"
     CDS             10531..11484
                     /locus_tag="ACHBKA_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM9444243.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11553..12902
                     /locus_tag="ACHBKA_000528"
     CDS             11553..12902
                     /locus_tag="ACHBKA_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9444244.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13085..13408)
                     /locus_tag="ACHBKA_000529"
     CDS             complement(13085..13408)
                     /locus_tag="ACHBKA_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444245.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13537..14700
                     /locus_tag="ACHBKA_000530"
     CDS             13537..14700
                     /locus_tag="ACHBKA_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9444246.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14770..16119
                     /locus_tag="ACHBKA_000531"
     CDS             14770..16119
                     /locus_tag="ACHBKA_000531"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9444247.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16164..16595)
                     /locus_tag="ACHBKA_000532"
     CDS             complement(16164..16595)
                     /locus_tag="ACHBKA_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9444248.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16983..19625
                     /gene="alaS"
                     /locus_tag="ACHBKA_000533"
     CDS             16983..19625
                     /gene="alaS"
                     /locus_tag="ACHBKA_000533"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM9444249.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19825..20421
                     /locus_tag="ACHBKA_000534"
     CDS             19825..20421
                     /locus_tag="ACHBKA_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9444250.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20543..21691
                     /locus_tag="ACHBKA_000535"
     CDS             20543..21691
                     /locus_tag="ACHBKA_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9444251.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21729..22142)
                     /locus_tag="ACHBKA_000536"
     CDS             complement(21729..22142)
                     /locus_tag="ACHBKA_000536"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM9444252.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22221..22652
                     /locus_tag="ACHBKA_000537"
     CDS             22221..22652
                     /locus_tag="ACHBKA_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM9444253.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22751..22984)
                     /locus_tag="ACHBKA_000538"
     CDS             complement(22751..22984)
                     /locus_tag="ACHBKA_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9444254.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23167..24837
                     /locus_tag="ACHBKA_000539"
     CDS             23167..24837
                     /locus_tag="ACHBKA_000539"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM9444255.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24984..25370
                     /locus_tag="ACHBKA_000540"
     CDS             24984..25370
                     /locus_tag="ACHBKA_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444256.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25357..26055
                     /locus_tag="ACHBKA_000541"
     CDS             25357..26055
                     /locus_tag="ACHBKA_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM9444257.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26060..26524
                     /gene="lspA"
                     /locus_tag="ACHBKA_000542"
     CDS             26060..26524
                     /gene="lspA"
                     /locus_tag="ACHBKA_000542"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM9444258.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26521..27432
                     /locus_tag="ACHBKA_000543"
     CDS             26521..27432
                     /locus_tag="ACHBKA_000543"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9444259.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27667..28206
                     /gene="pyrR"
                     /locus_tag="ACHBKA_000544"
     CDS             27667..28206
                     /gene="pyrR"
                     /locus_tag="ACHBKA_000544"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM9444260.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28220..29503
                     /locus_tag="ACHBKA_000545"
     CDS             28220..29503
                     /locus_tag="ACHBKA_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM9444261.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29516..30442
                     /locus_tag="ACHBKA_000546"
     CDS             29516..30442
                     /locus_tag="ACHBKA_000546"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM9444262.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30461..31741
                     /locus_tag="ACHBKA_000547"
     CDS             30461..31741
                     /locus_tag="ACHBKA_000547"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM9444263.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31746..32822
                     /locus_tag="ACHBKA_000548"
     CDS             31746..32822
                     /locus_tag="ACHBKA_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM9444264.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32827..36012
                     /gene="carB"
                     /locus_tag="ACHBKA_000549"
     CDS             32827..36012
                     /gene="carB"
                     /locus_tag="ACHBKA_000549"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM9444265.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36066..36842
                     /locus_tag="ACHBKA_000550"
     CDS             36066..36842
                     /locus_tag="ACHBKA_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM9444266.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36839..37771
                     /locus_tag="ACHBKA_000551"
     CDS             36839..37771
                     /locus_tag="ACHBKA_000551"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM9444267.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37768..38484
                     /gene="pyrF"
                     /locus_tag="ACHBKA_000552"
     CDS             37768..38484
                     /gene="pyrF"
                     /locus_tag="ACHBKA_000552"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM9444268.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38491..39117
                     /gene="pyrE"
                     /locus_tag="ACHBKA_000553"
                     /pseudo
     CDS             38491..39117
                     /gene="pyrE"
                     /locus_tag="ACHBKA_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            39144..39422
                     /locus_tag="ACHBKA_000554"
     CDS             39144..39422
                     /locus_tag="ACHBKA_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444269.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39460..40101
                     /locus_tag="ACHBKA_000555"
     CDS             39460..40101
                     /locus_tag="ACHBKA_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM9444270.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40144..40836
                     /locus_tag="ACHBKA_000556"
     CDS             40144..40836
                     /locus_tag="ACHBKA_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM9444271.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40833..41735
                     /locus_tag="ACHBKA_000557"
     CDS             40833..41735
                     /locus_tag="ACHBKA_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9444272.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41893..43599)
                     /gene="efbA"
                     /locus_tag="ACHBKA_000558"
     CDS             complement(41893..43599)
                     /gene="efbA"
                     /locus_tag="ACHBKA_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM9444273.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14029 a         7706 c         9499 g        12520 t
ORIGIN      
        1 tcgataaaaa aagatggtgc agacaaatta tgataattaa gcatactatc agataactca
       61 aaatgaagcc ttctttcaaa aaattattca aaaccatata ttgtattttt tatttgataa
      121 taacaatggg attattaaga agtggcacta aataaaagac tagtgtgtat ttagccgatt
      181 taattataaa caagtaaatg gataaagtat atgtaatgat aaaagaaaaa tggattttct
      241 aatttttaag ctaaatgtta gatcacacat gtatatgctg gatagtctta taaaaaatca
      301 tgtaatacag cttggtacat gatttttatt tgtagctgtt ttttacatag acacaaaaaa
      361 gaaaccacgc ttgtagacgt ggtttctttt ttactatctg tttccagaag ctgaggtgct
      421 gctggatggt gtatggtcat ttgcagcatc cgctccgcta gttgctggct cactggaaga
      481 agcaggagga gcagatgaag acggtgtcga gctttcttcg gatgaagaag aaggaaccgt
      541 tgttgaagac gctggctcgc ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt
      601 tgattcgctt tgactagtag agctagagct ttgagtcgtt gtctctgttg ttgagcttcc
      661 aggagtctgg acatagctgg aagatggaat agttgttgat ggtgtaattg cattcgatct
      721 agctgtatat tgatctttgt agtacagttc accgcccaca cgaattaatc cgcttggcat
      781 ctcccagtct gtatttgtca cgttagctga aacatactgc ataagctcgc gataaacatc
      841 agaggctacg tgtgacgatt ctgaagtgac aggtgtcatt tttttattgt aacctgtcca
      901 cacggaaatc gaataatttg gcgtatagcc cgcaaatagg atatctggat acaccccaga
      961 agaaatacca agttttgcat actcgtcatc tgtgtaattg gatgttccgg tcttaccggc
     1021 ttgatataac ccagcgattt gcgcattcgt accagttcct tcagtgatcg tatcttttaa
     1081 aatgtctgtg atcatataag ctgtttctgg agacatggct gtttttccat ctggctcata
     1141 agtttcttct gttccgtctt ggaagacgat tttattaaca tattgaggtt tgtaataagt
     1201 ccctccattg gcgaaagccg cataggctgc tgccattttt aaggaagaag ctccgtattt
     1261 tgtcccatct tgttcttccg tattactaga gatcgcattc gattgatgga tcgtactgta
     1321 ttcgatccca agatttttta agaaagaagc cactttatct gaaccgactt cattgaataa
     1381 tttgactgct ggaacattac gggataagta aagtgcttgg cgtaaagtaa tggtgcccat
     1441 atattgatta tcccagtttc ctacaggagt cgaagtccct tcatagttat acggtgcgtc
     1501 agtaatagtt tttccagtag aatatttcaa atattcaaaa gctggtccgt aatctgtgac
     1561 aggtttcatc gtagaaccaa aatcacggga agtattcact gctaagttgt tgcctaaagt
     1621 tacgtcttct gcgatgtgac gtccgccaat ctgggctttt actttcccgg tattggtgtc
     1681 gataagggtg gaggcaactt gcatctcgtc gtctgggtaa gagacatact gatccgtgtt
     1741 gacgatatca tatagtttct tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc
     1801 gtctgtgtag acattcttat ctgttttttc ttgtacttca ttgatgactt ctttgacata
     1861 attatcgacg atcttagtat tgtcatcgga ttgagttagt tcttgcagac catccgttac
     1921 cggtacatta acagcttgat catattctgt ttgagagatt ttttcgtttt gaagcatcgt
     1981 atacaaaaca gtatctcgtc ttttcttcgc ctgatctgga taaacataag gatcataggc
     2041 ggaaggggct tgaggcatcc cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa
     2101 tttcttacca aagtacatct cagaagctgt ctccatcccg tacaagccgt ttgacatata
     2161 cactttattt acgtagtagg tcaagatctc ttgtttcgat tttttttgtt ccaatcttac
     2221 agccatccac gcttcttggg ccttgcgttt cagagtctga tcctcggcac tggtggagaa
     2281 gaaggaaagt ttgatcaatt gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt
     2341 aaagttagag agggcagaac cgatgatccg aatagggtca accccgatgt gtttataaaa
     2401 acggcgatct tctaccgaca cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga
     2461 aattttttca cgcttttctg cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag
     2521 tttggaagat acggtagcgt ctaatttttt atccgtcaat tcgggcgcat cttttgcgta
     2581 ataccagaaa agtcctacac ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa
     2641 aaggatcttg ataaataatc cttttttatg tgttccagag gactttccag aacctttgcc
     2701 agaattcttc ttcacactct ttttaggagt aggctgtttc tgtcgtctag agcttctagt
     2761 ttgttcattt gccattcttg ctttctcctt caatagataa tgttgataaa tagaagtcaa
     2821 ccgcgtccaa ataaggaatc cgcggagaaa taccagtttt gatctctatt cctagttctt
     2881 cgatcaaaga taaaggtagt gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa
     2941 tcaaatgaac actattcaaa taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa
     3001 agcagacccc attttgttcg aggcatgctt tcatatgttc gatttgatgg gcatggaagt
     3061 ttttaaaggg aaaagatgtt ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc
     3121 ctcgatagac gccattatag tctgtagttg aggcttgggt aaagtaggct tccttgatca
     3181 cagcagcact tctcttagga tagtctactt tcacgatttg gataggggta ggctttttat
     3241 ggataaccgc caacccgcgg gaaagataat atttattact ttcattgatt gcttcttcaa
     3301 agcgcatccc ccgattgccg aattcagtga tctgatttct tttttcttga attttttcga
     3361 cgggagaggc attcttataa taaggttggc catttggata gcgcaatacc atctttaatc
     3421 acactcctag gccacattat agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga
     3481 taagtcaaaa gtaatctatg tgtctgggta tcgaagtttc gaactcggtg tttttaaaga
     3541 aaatgatcct aaaatccaag ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc
     3601 agaagaaggg ctggaatggg tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt
     3661 gcaaatagta gcggaattga aagaagaata tccggaacta tctcttggta ttttgtatcc
     3721 tttttctgat tttggtaata actggaatga agggaaccaa gaaaaaaaac aattagcaga
     3781 acaattagca gattatgtcg aagctgttag ccatcaaccc taccaatcac ctagtcagct
     3841 aaagaatcac actcgtttta tacttgagca tacagtgggc tgtctgctga tctatgatga
     3901 agaattccct ggaaaaacga agtatttttt tgaagatgca cagaattacc aagcagatca
     3961 tccctacgaa attcggctga tcagtatgga cgatcttcaa aattttagtg aataatcgat
     4021 attgtgtttg atttatcaac ttttttccta tacaatgaaa aaggtagaaa tgttattacg
     4081 taaatgaaaa ctagaggtgt aaatatggcg aatttggttt atagtcctaa agacatttta
     4141 caacaagaat tcaaaacaaa aatgcgcggc tatgatcccg ttgaagttga tgaattctta
     4201 gacaatatca tcaaagatta tgaagcttac agcaaagaac tccttgcttt acaagaagaa
     4261 aacgatcgat tgagtgcaaa agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca
     4321 cgcgtgcaac aaacagaggc accaaagagc gcagcagtga ccaattttga tatcttaaaa
     4381 cgcttgtcaa atcttgaacg tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca
     4441 gtcaaaccag cacaacctaa tccaaataac tacacaaatg cagacacaag cttagatgat
     4501 aatgaaaaaa cacgtcaatt ttaatttttg attaatgaat ttgcggtttt cgggtaatcg
     4561 cggtctgact tgatcaggct gaggaaagtc catgctcgca caggctgtga tgcctgtagt
     4621 gttcgtgctt agcgaaacca taagctaagg tactctttgg agtaacggca ggaaaacaag
     4681 ctaaggcttc ggctatgctt aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg
     4741 aaacccatcc ggtggaacgc ggtaaacccc tcgagcgagc aacccaaaca atggtagggg
     4801 cgctttttgt ctggaagaag aacaggacaa aaaggcagta taactgcaga tagatggtta
     4861 ccgctttgtt ttttctccct ggaaaagcaa agtacagaac atggcttata gaaaaccgca
     4921 aacatcaggg aattaggtcg tgacaaaagt aatgcgttat aagaaacaag aaaaacattt
     4981 gaaagctatt tttcaatact tcagatgaat cgttcttatt acaaaacaaa cttttgtatc
     5041 accggttatt cggaatttga gtaaaagtgt ggggtaggac tgatgtccca cctccttttt
     5101 ttataaataa ggagattgtg tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg
     5161 gcaagcgggc tagaagcact ggttggtaaa gaattaagag atcttggaat cgaatgccaa
     5221 gttgagaacg gacgtgcacg ttttgaagga acaatggaaa cgatcgctac agctaattta
     5281 tggttacgta cagcagatcg agtaaaaatc gtggttgggg aattcgatgc gtatacgttt
     5341 gatgaattgt ttgaaaacgt aaaagcattg ccttgggaag attttcttcc actagatgca
     5401 gcttttcctg tagctggaaa atcgatcaag tcgaaactat atagcacacc tgattgccaa
     5461 gcgatcacaa aaaaagcaat cgttgaacgt ctccgtacct attatcaccg tccagcttct
     5521 gtaccactga cggaaactgg cgcacatttt cagttggaag tagctttgct gaaagaccat
     5581 gtgatggtaa ctctggatac gacaggacca agtttattca aacgtggtta tcgcttagaa
     5641 aaaggtggag caccattgaa agaaaacatg gcagcagcac ttattatgtt gacaaattgg
     5701 cgaaaagacc gtccatttta tgatcctgta tgtggttcag gtacgctttg tattgaagca
     5761 gccttaatag gccataatat cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg
     5821 gattggttca gtcaagaaat catggaaaat gttcgatcag cagccgaaga aaaagcagat
     5881 tatgatattg aattagatat tactggttca gatatcaatg gacggatgat tgaaattgcc
     5941 aaagccaatg cagaagaaat cggattaggc tcttcgatta cctttaagca acaagcagtc
     6001 aaagacttca aaacggataa agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa
     6061 cgtctaggag aagaagaaac tgttcggaaa ttatataaag aaatgggcga tgtattccgt
     6121 ccattaaaaa catggagtaa atatatcttg actagcgact tagcgtttga ggaattttac
     6181 ggacaaaaag caaccaaaaa aagaaaattg tacaatggtg cattgcgtac ggatctattc
     6241 cagtattggg gaactcgtcc gcctagaaaa aaagagaaag acgaataatc ttactaattg
     6301 attcattcaa ttggatgttt ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa
     6361 gtaaaagaaa tcaatcttct acaacaagct ttaggaatcc tcgattggga tacacaaaca
     6421 ggtatgcctg aaaaagctag tgcataccga agtgaagtag acagctatct ttacagcatt
     6481 tatttttcaa aaaaaatcgg tccaaaaatc aaagaagcca ttcgctattt ttctgaacat
     6541 ccggaagaat tgtcggaagt tggaactgcc gtttttgcca aagtaaaaga agagtatgat
     6601 ttggaacatc gcgtaccgga agatcttatg agaaaatata cagctgccgt ttcttcagca
     6661 cacggacaat ggcaaaaagc tagagaaaca caacaatttt ctgattttca acacgcattg
     6721 aatcaaaata ttgaattaac aaaacagttg ataccttact ggagaaaaga tgaaaagacg
     6781 aattatgatg ttttgctgaa tcaatacgaa cccgggatga ctgtcgaaat ccttgataag
     6841 gtatttgatc agcttaaaga aggaattctt tcgatccgcc gtacgctcca agaaaaaggg
     6901 actgaacctg aaacagactt tttaaatcgg agaatgacga aaaaacaaca gaagagattt
     6961 gtagtgaaag taatcgaaca acttggttat gattttagtc gtggtcgttt agatgacact
     7021 gtccacccat ttatgactgg aattaatccg aatgatgcac gaatcacgac acgatggaac
     7081 gaaacagatt tcaaaatggc tgtttttgga gtgatccacg aggctggtca tggtatgtat
     7141 gaacaagaca tcgatgataa atatgcgtat acaccggttt atgaaggtac atcaatgggt
     7201 atccatgaat cacaatcgtt gtttaacgag atcattattg gaagtagtcg cagcttctgg
     7261 aaaaagcaat atcctttctt tcaagaatgt gcagaaggaa cattcgatga tattcctttt
     7321 gacaaatttt atcgttcatt gaaccataca aaatcaagtt tggttcgtat cgaggcagac
     7381 agtttgactt accctctcca tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat
     7441 gaaggggtag atgttgctga actacctgaa atatggaatc aaaaatatga agagtatctg
     7501 ggaatacgac caacaaatga tgtggaaggc atcctacaag acgttcattg gtcaggtgca
     7561 agctttggtt attttccctc ttatgcatta gggtacatgt atgcggcaca attacttcat
     7621 tctttgcagc aagaaataga tgtggaaaat gttctagcgt cagacgatta ttctccaatc
     7681 aaaaactgga tgtcagagca tatccatcaa tatggtgcat ctagaaaacc aaatcaatta
     7741 atcatggatg caacacatga accgcttaat ccgcaatacc tgatcgacta tatgaaagac
     7801 ctgtatttta atgtatatca ggtaaaataa aatacaattt acaaagatag ctgaaattca
     7861 tctaatctat agatgttttc agctatttac ttaagaaaga agtggaacga atggataaat
     7921 tacaagtgat cgctgattat agtatggacc aattagcgca tgatcaaaca ggacatggga
     7981 gcgatcatac caaaagagtt gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg
     8041 cagaccgctt tgtgacgctt gcagcagctt acctacatga tacgattgat gataaagtcg
     8101 ttaaaaatga gaacgaggca aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag
     8161 tagaggaaca gatctcgatg attttttcca ttatcgaaaa tatgtccttt tctaaaaatc
     8221 tctctgaagc ggccgaacta tctctggaag gtaagatcgt tcaagacgct gatcgtatcg
     8281 aagcattagg agcaatcggc attttgcgta ctgcttattt cggcggtgga catgggcacc
     8341 cgatttttga tcctgaactg tatccgcaaa cattcaagga caaagaaaat taccgaaaag
     8401 gcacgacagt gatcaatcat ttttatgaaa aactgttctt gctgtctgat aaaatgaata
     8461 ctgattacgg atatgaagaa gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag
     8521 aattttttct ggaatggtct attgacgatg aaaattttac tccagacaat tggatcaaaa
     8581 ataacaaaaa atgatggagg ttgtgacaga agtggtcagc tccaagatat ttcggatcat
     8641 tttccgtaaa agttacttct ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag
     8701 ctccgagtca caaggaacaa tttcaaaaaa tagcttctcg tgtttgatga atattgtaac
     8761 ttgtgattcg gaattggcag gagaacaccg tttattcgga agaataggac tgtgacaaga
     8821 cttttgtcac agtccttttt tcgaattcaa caagtaaaac ttttaagaaa tattcagaga
     8881 gtaaaattaa aaattgctga taaacgaaga tggaaatttt atgtattttt attggtctat
     8941 gcaataaata aattaaatat tcagaataca attgatttta acgaaagggc ttctatcaat
     9001 ggttgtgttg tatttatctg cccaggtaga gtaaaataga tcattatcta aaaggtaatt
     9061 gttcatacta tataagtcat ttgaaagtaa aaagatactg taaataaaaa atgtggttaa
     9121 ataattctca taatcagtgt aagcgttatc aaaaatatag catacttgct ttgtagatga
     9181 atgacacgat atatgtttgg tatgttctgc aagacagatg aatatggagg tggagacaat
     9241 gtcttccaaa gaactaacaa tgtatttaga gaaagaattg gatcaactga aagaaaaggg
     9301 gttatacaat acgatcgaca tactagaaag tgaaaatggt gcttgtatca tcgtagacgg
     9361 aaaaaagatg atcaatctag catccaacaa ttatcttgga tttgccaatc gcgaagaatt
     9421 gaaaaaagca tgtattgaag caaccgaaac ttatggtgta ggagcaggag cagtccgaac
     9481 gatcaatggt tcgctgaaaa tccatcagca attagaagaa aaaatcgcag aattcaaagg
     9541 gacagaagca gcaatcgcct ttcaatctgg attcaactgc aatatgggcg ctatctctgc
     9601 agtaatgaca aaagaagatg ctatcttatc ggatgaatta aaccatgcat ctattattga
     9661 cggttgccgc ttatccggag caaaaatcat ccgaattaaa catcaagata tgaaagacct
     9721 tgagaaaaaa gcaaaggaag cgatagaaag taagaaatat aaaaaaatca tgtatatcac
     9781 agatggtgtt ttctccatgg atggagacat tgcacgattg ccggaaatta ttccgattgt
     9841 tgaaaaatat ggactgatca cctacgtcga tgatgcgcac ggctcaggcg taactggaaa
     9901 aggtgcagga accgtcaagc attttggatt aagcgacaaa atcgatatgc aaatgggcac
     9961 gttatcgaaa gctataggtg ttgttggggg ttatgtagct ggatcgaaaa cactaatcga
    10021 ctggttgaaa gctagaagtc ggccattcct attttctact tcgctaactc caggtgcagc
    10081 tgcagctgca ttggcttcga ttactttgat gcaagaacat ccggaacttg tcgaaaaagt
    10141 ttgggaaaat gctaattatt ttaaagaaga attgaaaaaa gtaggctata acatcggtat
    10201 gtctgaaaca cctattactc cagtcatttt gggagacgaa aaagtgactc agactttctc
    10261 taagaagctg atcgaacatg ggatttatgc taaaccaatc gtttatccaa ctgtgcctct
    10321 tgggacaggc aggatcagaa atatgccgac tgcggaacat acgagggaaa tgcttgatga
    10381 agcagtagca gtttatcaaa aaataggaaa agaaatggaa attatttaac taaatttagt
    10441 tttttcgtct atttaaaatt agagaacatg ttcacgcata tattgttaat catctgaaga
    10501 tagtagtaaa aaagatcagg gaggatcatc atgaaaaaag tattggtgac aggatgttta
    10561 ggacaaattg gttcagagtt gatcacacgg ctacgtcaag aactaggcag tgaaaatgtc
    10621 attgcgacag atatacgcat gcctgaagtc attgagaaaa acgatatctt tgagatccta
    10681 gatgtaatgg attacgaaaa aatgcgcagc atagcagaac aatatcaagt ggacacattg
    10741 attcatttgg ctgctctttt gtcagctgtt gcagaagcaa aaccacaatt agcctggaat
    10801 ttgaacgtga ctgggctggt caatgcattg gaagtcgcta gagaattcca gctgaaattt
    10861 tttacgccaa gttcaatcgg cgcgtttggt ccaaatactc caaaagatca taccccacaa
    10921 gatactcttc agcgtcctac aaccatgtat ggggtaacga aggtagctgg agaactacta
    10981 tgtgattatt attatgtgaa atatggcgta gatactcgtg gtgtccgttt ccctggattg
    11041 atttcttatg caaccttgcc aggaggcggt acgacagact acgcagtcga tatttattat
    11101 tcagcaatca aagaaggaaa gtacactagt ttcattgatc aaggtacatc aatggatatg
    11161 atgtatatgc cagatgcgat cgatgccatt ataaaactca tgaatgcaga tcctgatcga
    11221 ttggttcatc gaaatgcctt caatatatcg gcgatgtctt ttgacccgga agagatcaaa
    11281 gcatctattc aaaaaatcat accagatttt gaaatggatt atgatgtgga tcctgtacgt
    11341 cagtcaatag cagaatcttg gccaaattca ctagatgttt cttgtgcgca agaggaatgg
    11401 ggcttttcgc cgaaatacga cttagatgtg atgacaaagg atatgttgga aaaactgaaa
    11461 gaaaaattgg tagagacggt ataaaacaag aaccacatcc aaaaagtttt ttatactttt
    11521 gggattggct atggtatgaa aggggacaag ggatggaaga atatcaagcc acgccagtaa
    11581 aagaagtaaa aatagaaaat gaattaaaac gtacgatggg attttttact gctttatcga
    11641 ctgttatggg cacagtgatc ggtgcagggg tattttttaa agccgcaagc gtagcagaag
    11701 ttacaggatc cgccagtttg catatgtttt cttggtttct cggtggtatg atttctgtct
    11761 gtgcaggact aacaggagct gaactggcag ctgctattcc agaaacagga gggatgatta
    11821 aatatatcga acggatttac ggaaatacag cagctttttt attgggctgg gcgcaagtcg
    11881 tgatttattt tccagcaaat gtagcagcct tgtcgatcat tttcggtaca cagttcgtca
    11941 atctatttgg tctttcccag tcaatgattg ttcccgtagc tgttacagct gctgtatcga
    12001 tcctgttgat caatttttta ggttctaaag ctggaggggc ttttcagtcg attacgcttg
    12061 tctgcaagct tattcctttg tttgtgatcg tcattttcgg tttgttccga caagaaggag
    12121 tagacttcca actgtttccg atccaagcag gagaaaatct ttcatttttc tcagccttgg
    12181 gagcgggctt gctagcaacg atgttcgcat acgacggttg gatacacgta ggaaatattt
    12241 cgggagaatt gaaaaagccg gcgaaagatt tgccaaaagc aatttcttta ggaatcatcg
    12301 gaatcatgat cgtttattta ttagtaaatg cggtgttctt aagaaccgca tctatcgacg
    12361 gagtcgcagg aaacagcaat gcggcaagtg acgttgcaaa aatgattttt ggcggcttcg
    12421 gtggaagact ggtcacagtt ggtatcttga tttctgttta tggaaccatc aatggctata
    12481 cccttactgg aatgcgtctc ccttatgtta tggcaaaaga aaacaacctg ccttttagca
    12541 agctctttgc taaacttcat gataaaacta aagttcctgt agccgcaggt atcttagagc
    12601 tagtcatcgc tattggcatg atgatgatcg gtggttttga tacactaacg gatatgctga
    12661 tctttgtcat ttggattttt tatacgatgg tctttgttgg tgtcattctt cttcgtaaaa
    12721 aagaacctga tttgtttcga ccatacaaag ttccaatgta tccgtttatt ccactagtag
    12781 caatcattgg agggactttt atcgtatcaa gcacgcttat cactcagacc tttttagctt
    12841 cgatggggat agtattgaca cttgctggta tcccgattta tctctattta aaacgtagat
    12901 agctaaaaca ttcgaatttt attagaactg cacttctctg ccaaacaaaa tattttattt
    12961 aaatgaggag tacgtggaag aaatgtcaaa agtactcgtt aattttgatc acgtaaatgt
    13021 cgttttgtct acgatgaaat agcaaagtaa aagacagaaa tcgggctcaa ccgatttctg
    13081 tcttttactt tgatttgcgt aactgaacta ttcggcgtac gccgcgataa atatttaata
    13141 aaaccaagca accagaaatg aaaatgggaa gccaaatcag atagacaggg tagccaagca
    13201 ctttagacaa agcatgtgga gtcattttat ccatttcaag aagtgcttgc tgccaagtct
    13261 gaagattgaa gactagcatg aaacccgaaa taatcaggag aataaatcct aaataatgaa
    13321 ttgtagttct ttttcttaca taagcgatcg tagcataaag gaacagtaaa aaaataggta
    13381 cgccaaaaac aaagtacata ttcagcatct cgacaccccc tttgtgttta tcttacttta
    13441 ctttgaaaga aatgagaaat aatagcctcg ttacttgctt gaaacagcaa aggactgcta
    13501 agataagaga gatacaaaga atcaaggagg agtcatatgg caagttatat cgaaccgatt
    13561 cgttttgcgc tgattgtttt tccattttta gccttagcga tctcatctgt tttttttatt
    13621 tatgaatatc gaaagtatgg aacatttcta gtaacaaggg cagttatttt gtattctttc
    13681 gttttttatt tattgtgtgc gtattttttg gtgatcttac cacttccggc aaaggagacg
    13741 gttgcccaaa tgacagggcc aaagatggag ctgcatttat ttgcttcatg gcaacatttc
    13801 agagacaaca cggtattggt cttgtctgaa cctagtacct acctgccggc aatgaagcag
    13861 agtgtatttt tggaaccagc ttttaatgtt ttattactca taccatttgg catttattta
    13921 agatattatt tccggctttc ttttatgaaa acgatgattg ctagtttctg cctttctcta
    13981 ttttttgaat tgacgcagct aagtggactt tattttatct acccaagacc ataccgctta
    14041 tttgatgtga atgatttagt gacaaataca ttgggtggaa tcattggttt tgtcgttacg
    14101 ccgttattta catttatgct tcctacaaga agacgaatgg atgaactttc ttatgaaaaa
    14161 ggaaagactg tctcattgac tcgacgcttg gtcgcttatt tgatcgactg gacattttta
    14221 tctcttatta atttggtagc ggcgattttc tttaaaattg cgttcgattc aggggcagtt
    14281 acttctcata tctggtggct tttattagaa gtaggggtat actttatgat tgtaccgttt
    14341 ataaccaatg gatggacact tggcaaaaaa attgtgcgta tccgtttggt ggaagaagac
    14401 cgcgaacgaa tttctttaaa agcattgatg atccgctacg ggtatctgta ctttcttttt
    14461 tatggtttaa gctggttggg gcagttgctt cagcaaatca tgaattcgac aacgaatacg
    14521 gtgttgcaga tgagtgcagt attcatttct ctcggtgtct tgttactaca aggtattttt
    14581 attttaaata tcttgctatc atttttgaga aaaaaacgga ttctgtttta tgaaaaagct
    14641 agtcacacat atactgttag cacgattggc caaacaaagg atgatgattc tcgaaattag
    14701 ctgagcaaaa acgctttgtc tgtgttataa tgccaaagtc gtgataattt aaagagaaga
    14761 ggtgcacgaa tgaataattt tgatatattc cgcttcaaac cgtttatcaa tgaagcgtta
    14821 accgcaaagg gcttcaaaca gcccacagag gtccaggaac gtttgattcc tttaattgca
    14881 aaaggaaaaa atatcattgg gcaatctcaa actggtacag gaaaaacaca tacgtttttg
    14941 ttgcctttgt ttgaaaaaat caatgcagaa aaagaggaag tacaagttgt tattacagct
    15001 ccaagtaggg aactagctgc acagatctat gaagcagcta cacaaatcgc taaattttct
    15061 acaccggaga tccgtgtctc gaattttgtc ggcggtacag acaaacagcg tcaattaaat
    15121 cgtctgcatc accaacagcc acaggttgta gtaggcacgc ctggtagaat attagacatg
    15181 atgaatgaac aagcgctgaa gattcacacc gcatttgcat ttgttgtcga tgaagcggat
    15241 atgacattgg atatgggttt tttaaatgaa gtagaccaaa ttgcaagtcg attaccagaa
    15301 aagctgcagt ttttagtttt ttctgccact attccagaaa aacttcgtcc attcttacga
    15361 aaatacatgg aaaatccaat cattgaagaa atcaaaccaa aagcagttat ttctgaagac
    15421 attgaaaatt ggctgatttc aacaaaaggt aaagatgaga atcaaatgat ttatcaactg
    15481 ctgacaattg gtcatcctta tttagcaatc gtatttgcca atacaaaaca gcatgtggat
    15541 gaaattgccg attacttgaa aaaccaaggc ttgaaagtag caaagattca tggagacatc
    15601 accccaagag agagaaaacg agtgatgcga caagtacaga atctggagta tcaatatgtt
    15661 gtagcgactg atttagctgc tagagggatc gatatcgaag gtgtctctca tgttattaat
    15721 gcagaaattc cagaagatct agacttcttt atccatcgtg tcggacgtac tggacgtaat
    15781 ggcttaaaag gaacggccat tacgttatat tcgccaaatg atgagcaagc cattgatgcg
    15841 atagaaaaat tgggcgtctc atttgaacca aaagaaatca aaaacgggga aatcgtcaaa
    15901 acatacgatc gtaatagacg tgccaaacga gaaaaatcaa aagatgtatt agatcccacg
    15961 ctgattggtc ttgtgaagaa gaaaaagaaa aagatcaagc ctggttataa gaaaaaaatc
    16021 gattgggcaa ttgcagaaaa aaataaaaaa gaacgcaaaa tcgaacgcag acagcaaagc
    16081 cgtactgctc gtaaaaataa gaaaaattca cataaataaa aaacaagagg ttgtgacaaa
    16141 aggcgtgtca caatctcttg tttttaagct agactttttt tcattccgat acaaagtact
    16201 tcatcttcaa taaaaggttc tgtgcaagta tgataccctt gtttttcata aaaatctaca
    16261 gccgtcattt cagctactac ataactagtc tgacaacctt ctgaaaccgc ccgtttttca
    16321 gcagcttcaa gcaactgacc gcctaaccct tttcctcggt attcttttgc tacgcaaaat
    16381 cgttccggct tgaagcaagt aggtgttttc ttttcataac gcaaagtagc tactggtctt
    16441 ttttgatcaa gaaatagata ataggtttgt tgcgggatat cttttccgtc gaattctagt
    16501 tctgcaggaa tcccttgttc taatacaaaa acttgatatc tcaaaaagca gctagctgcg
    16561 cgtatccatt catccgctcc aactattttt ttcatcatat tcctccttgt catgaatagc
    16621 agagatgtat tgattccttt attcttatcg taccaaaaat cgtggagtat gaccaaaagg
    16681 aaatgaaaat aaaaattgag atattgacag tgatcaatag aatgcgtata ctaattaagt
    16741 acgaaaagga tatgatttgt tttttaagac tcacagaaaa ttctttcttg gctgaaaaaa
    16801 gaatagtcac taatcaaatt gctccctttt tctattttgg tagaaatacc gacgcagctc
    16861 agcgttatcg agtttaagag gtaatgatta cacatcattg caatcaaggt ggtaccgcga
    16921 gttttcgtcc ttggctgtga tggtgttttt ttgtatttaa tttatagaaa gcaggtaatg
    16981 acatgaaaca attaagtagt gcacaagttc gacaaatgtt tcttgatttc ttccaatcta
    17041 aaggacatac gattgaacct agtgcgtctt tgatccccgt gaacgatcca accttattgt
    17101 ggatcaactc tggtgttgca acattgaaaa aatattttga tggttcagtt gtgccagaaa
    17161 atccaagaat cacaaatgcc caaaaatcta ttcgaacaaa tgatatcgaa aatgtcggta
    17221 aaacagcacg gcaccatacg atgtttgaga tgctgggtaa tttttcgatt ggtgactatt
    17281 tcaaaaatga agccatccac tgggcatggg aatttttgac tgatccaaaa tggatggcat
    17341 tcgatccaga aaaactttat gtaacagttt atccaaaaga tacagaagca aaacgcatct
    17401 ggcatgaaga agttggcctg ccactagatc acattattga tgtagaagac aacttctggg
    17461 atatcggtgc aggcccatgt ggtccggata cggaaatttt ctatgaccgt ggagaagctt
    17521 ataacgattt agcagaagac gatcctgaaa actatccagg aggagaaaat gaacgttatt
    17581 tagaaatctg gaacttagta ttttcagaat tcaaccataa gcctgataac acatttgaac
    17641 cacttcctca taaaaacatt gatacgggga tgggattgga acgcatggtc tcaatcgttc
    17701 aagatgcgtc aacgaacttt gagacagact tgtttatgcc tattatccac gccgtagaaa
    17761 agctaagcaa acaagtaaaa tatggtgaaa acccagtaac agatatttct tacaaagtaa
    17821 tcgcagatca tattcgcgcc ctttcatttg ctattggcga cggtgcattg ccatcgaatg
    17881 aagggagagg gtatgtctta cgccgtttgc ttcgtcgtgc tgtcatgcat gggaaaaaat
    17941 taggtattga acaagcattc ttatatcgtt tagtccctgt tgttggggaa atcatggtaa
    18001 gttattatcc tgaagtactt caacaaaaag aatttattga aaaagttatc cgtacagaag
    18061 aagaacgttt caatgaaaca atcaatgaag gtttggaaat cttaaatcaa gtgatccaag
    18121 aagtaaaaaa cgaaaacggg acgactctag caggaaaaga catcttcaaa ctatacgaca
    18181 catatggctt cccggtagaa ttaacggaag aggtggcaga agatgaagga ctgaaagttg
    18241 atcatgctgg ttttgaaaaa gaaatggatg cacaacgcga acgtgctcgc tctgcccgca
    18301 gtactgaaaa atcaatgggg gtacaatctg ctttattgac agatatcaaa gtagaaagta
    18361 aattcattgg atacacagaa caagaatcag atagtcaatt attggtcatc gttaaaggtg
    18421 agtctctttt gaatgaagtc gcagaaggtg aagctcaaat gatctttgat caaacacctt
    18481 tctatgcaga aatgggtggg caagtagcgg atcaaggatg gatcatgaat gaagtaggcg
    18541 aaatcgtagc agttgttaca gatgttcaaa aagcgcctaa tggtcaattt atgcacaccg
    18601 tagaagtaaa agcaccaatg agtgaaggac aaacctatca tctattgatc gatcaatcga
    18661 tgcgcaatcg gatcatcaaa aaccatacag caacccatct actgcacaag gcattgaaag
    18721 aagtacttgg cgagcatgca aatcaagctg gttctttagt cgctcctgga catttacgat
    18781 tcgactttac ccactttggt caaatcactt cagatgaatt agctgaaatg gaaagaatcg
    18841 tcaacgaaaa aatctgggaa gctatcccag ttgaaacaat tgaaacagat atcgatacag
    18901 ccaaaaacat gggtgcaatg gctttgtttg gtgaaaaata cggacatgac gttcgagtag
    18961 taaatattgg cggctggtca atcgaactgt gcggaggcac acatgtagca aatacagaaa
    19021 acatcggaat cttcaaaatc gtttctgaat caggaattgg tgcaggcgtg cgacgaatcg
    19081 aggctgtaac aagtaaagaa gcgtacgaac tacttgaaga agaagaaaaa caactaaaac
    19141 aagtagcaac aattgttaaa tcacctcaat tgaaagaagt tgtttcaaaa gcggaacaat
    19201 tgcaacagca agtaagagag ctacaaaagg aaaatgaaca attagctagc aagttagcga
    19261 atcaacaagc tggtgatgta ttcaaagatc cagaaacgat caatggaaaa acaatcattg
    19321 cagcacaagt aaaagtgaaa gatatgaatc agctgcgtca attagctgac caatggaaac
    19381 aaaaaaatat ttcagatatt cttgttttag caaacgaaca agaaggaaaa gtcagtctgc
    19441 tagcggcaat gacgaaagaa tcaaatgaag ctggactaaa agctggcgac ttgattaaag
    19501 caatcgcacc gaaagtaggc ggtggcggtg gtggacgtcc agacatggca caagctggcg
    19561 ggaaaaatcc tgctggtatt ccagatgcac ttaaagctgc gaaagaatgg gttgcgcaac
    19621 aatagggcgt ttacgtttga cagaaaaaca aaagttcaaa cattgaacga aacatgatta
    19681 tgggggcgaa gacaaacaag tcttgccctc atactttttt caattggatg gcgcatctaa
    19741 tcaaatggtc ggattcggat ggtctttagt tgacgaaagt gacagaaatt tttacgatta
    19801 gtataaataa aaaggggtga aaaaatgaat agtagaaata agacctttcg actagtctta
    19861 cgtgcgatct tgttagcaat catcatcgta caagcgatgg ttccttggct ggggtttatt
    19921 ccattaggat tcattagttt aacaattatc catatcacag taattattgc tgcagtagta
    19981 ttaggcccaa aagatggcat ggtgattggc ttgttttggg gaatcgctac aattgttcgt
    20041 gcctatgcca tgccaacaac gccatttgat acattagtgt tcacgaatcc gattatctcg
    20101 gtagttccca gagtacttgt tggcttagta gcaggcttgg ttttccactg gatttatcaa
    20161 aaaaataaat caataacgat cagctcagcc tttgcagggg tactgggttc attggtcaat
    20221 acagtcttag tgttaggatt catgggatta ttttatacag gtgcaacggc agatgcctat
    20281 ggtgtagact ctgctctttt atttaaaacc ttagcagggg ttgcagcaat caatgggata
    20341 ccagaagcaa taggtgcggg aatcataaca ccattactag caaaagcgtt atttgcagca
    20401 acaccgttga aaccagaata atgaccatct tttaatagta ataggacgca cgatagatag
    20461 aaaaaggaag agaggagagg aaaacaatcg tcctctccat ctttttacct gagcaacaag
    20521 caaactagaa ggggtaaaaa acttggctaa gaaacaagaa gaaaaaaaga aacaaatacc
    20581 gcaacagcgg acaaaaaaag gagtactagc gggtatctta ggattggtta cgaatctgat
    20641 cttgtttgtc tctaagctaa tgatcgggct tttttcagga agtgtctcca ttatggctga
    20701 tgcagtgaac agtctatcag atactgcttc atccatattg actttgatcg gatttaaaat
    20761 tgctgcaaag ccagcagatc aggaacatcc ttatggacac gaacgcttcg aatatatcag
    20821 tgggttattt gtttcgatca tcattactta tgtaggattt caatttttag agtcttctgc
    20881 tgaaaaaatc atccatccag aaaatgtctc tttaacaccc atcgtctttt ttgtcctaat
    20941 catttccatc tttttgaaat ttgcacaagg caaaatgtat caggttattg cgcaatccat
    21001 tgattctgaa acattgagag ctacagcaac agatagttat aatgatgtct ttactacact
    21061 ggctgtattg ctttcagcag caatcgaatg ggtaactgga tggcgtattg atggatatat
    21121 aggttttgct ttagcattat atatccttta tagtggattg acgatgcttc gaaattttgt
    21181 atacgaacta ttaggaagtc gcccaacgcc agcagagatt gaagaaatgg aagagcattt
    21241 aaatgcttgc gatgatatac ttggctatca tgatttattg gtgcataact atggacccaa
    21301 taaacgcttt gcatcagtcc acatcgaagt agatgaatca atgaatttga atgtggctca
    21361 taagattatt gatctgatag aaaaagactt taaaaagtct ttggatgtgg aacttgtctg
    21421 tcacttggat ccaatgcctg ttgataacga aaaatactat aatatcttgc ggcagttaaa
    21481 gcaaattctt cttgaaacag gtacagggct taatattcat gattttagag taattcatca
    21541 aggaaaaacg ctgcaatttg atgtagtggt ccctgagaaa ttccagtttg ttgatacaga
    21601 actagaaaga atcttacgta gtcaaataga agaaaaaatc ggagattacc aattggaaat
    21661 tacgtttgat cacaattatc ttttatatta aatgagaaac tataaaaaga ctccttaaag
    21721 tttttaattt aaagagtctt tttttctttt aattgtttat ggagagcctg acgtgcaaga
    21781 ttatctgctc ctttattttt gttttcgggc aaccattgca ggatcaataa agaaaattcg
    21841 gatagaagtt cttggattct ttgaaggtag ggaaggaaga gctcgttctt cgttgcgttt
    21901 ttgtcgaccg tagaaactag tgttttgctg tctgaaaaac agaaaatcgt ttcattctgt
    21961 aagtcgtttt tctttagaaa ttctaaagtt tttaaaaaga cttcgaactc tgcttgatgg
    22021 tttgtcccta tagaaagcgg gatagatagc tgttcttgtt taccttgata gacaatcaag
    22081 attccacctc cactaggacc agggttgcct tttgtagagg catcaatgta tacttttaac
    22141 ataaaaagag attctccttt ttgttcaaac taatgttatg gtaagattat atcattactg
    22201 aatcggtgag aggttagaga atgaaaaaaa atattcgatg gcagcctgaa ctggcacata
    22261 cgatcatcta ttggtcattg accttgatca ttctgttttt tagtctgatt ctttctttag
    22321 aaaacacagg tccttattgg aaaagcaatt ttgttttagg tatttttctt gtttttctat
    22381 ttctaggttt gcggcgttct tttattttaa aatcaaactc tgtgattata cgttatgctg
    22441 ctttttggaa agaccgagaa atatctgtcg gacagattcg atctttgacg ccaactaata
    22501 ctggggtgca aatgatgttg caagatgaga aagccccatc tgttttcatg atgcgaaaaa
    22561 aagatcaagt agtccttttt gatcattttg aaaaaaacgg acccaaatca gaatcaattc
    22621 cttatcctta tacgaaaaaa agaaaaagct aattttaaaa atcagaacct aaaaaaacga
    22681 atttttacag gaaaagaaaa gagtgagacg tacggttcat cccgtttagc gtctcactct
    22741 atttgtttat ctattcatgc atactagtat cggtagtctg aacgttgcct ttcttgacat
    22801 gggcagcttg tgttcctcga ttcccttcga tgatctcaaa ttcgaccact tgattttctt
    22861 ccaaagtttt gaatccctct tcttcaatcg cagtaaaatg tacaaaaatt tcttctgtgt
    22921 catcataact gataaagccg tatccttttt tgttgtcaaa ccattttaca attcctgtgg
    22981 tcaaagcact cacgaccttt ctaaagtctg atcatagcct tcattataaa gagtatttat
    23041 aagtacgtca aataaattaa aaacctgtaa ttttatgttt tttttctgaa taaaaaaagg
    23101 tataattggc atatcttaac aaagaagata ttttgttgaa taaagttcgt gaaaaggggt
    23161 aatcaaatga agaatgatct gcaaatttca caagaaacag agctagaacc gattgttgaa
    23221 gtggctagta aattagatct taaggaagac gatttggaac tgtatgggaa atataaggcg
    23281 aaaatcaatt tttctggaat caatcgatca aaggataacg ctgaggggca tttgatctta
    23341 gttacttcca tcaatccaac accagctggc gaaggaaagt caacgatcac tgtagggtta
    23401 ggagacgcgc tcaataaaat caataaaaaa tctgttattg cattgcgaga accctcactt
    23461 ggtcctgtga tgggaatcaa aggtggtgct gcaggcggag gatatgcaca ggtattgcct
    23521 atggaagata tcaacttgca ttttactggt gacatgcatg ctatcacaac agctaataat
    23581 gcattatctg ctcttttaga taatcatatt catcaaggaa atgaattgaa catcgatgcc
    23641 cgccgagtga tttggaaacg ggtagtcgat ctcaatgatc gtgaactgcg taaagtaatt
    23701 gttggcttgg gtgggccaat ccaaggtgtt ccaagagaag atgggtttga tatcacagtt
    23761 gccagcgaaa tcatggctat cttgtgtctt gcggcagacc ttgaagattt aaaggctcgt
    23821 cttgcaagga tcgttgtcgg ctatacattt gaccgtcagc ctgtgactgc tggagacttg
    23881 aaagcagaag gcgctctggc attactgctg aaagatgcaa tcaagccgaa tcttgtacaa
    23941 acaatttacg gaacaccagc ttttgtccac gggggaccgt ttgccaatat cgctcatggc
    24001 tgcaatagta ttttagctac caagacagca ttgagattag cagattacgt tgtcacagag
    24061 gcaggatttg gtgcagatct tggcggagag aaatttttag atatcaaagt acctaacttg
    24121 aaaaaagcgc cggatgctgt ggtgattgtc gccactgtcc gtgcattgaa aatgcatggc
    24181 ggaatgaaaa aagatgaact taaaaatgag aatcttgatg ctttgaaagt aggttttgca
    24241 aatttaaagc gccatatacg taatatggaa caatatcagc tacctgtgat cgtcgcaatc
    24301 aatgaattcg taacagatac agacaatgaa ctcacactgt tggagcactt gtgtgaaaat
    24361 caggggattc ttgccaagag agcgagtgta tgggcagatg gagcagaagg cggcgttgat
    24421 ttagcaaaag ctgtagtacg attgattgat agaaaagaag ctaattacaa gccattatac
    24481 cgcctagaag aaacgataca agaaaagaca gaaacaatcg tcaagaaaat ttacggagga
    24541 aatggtgttg ttttcagcaa aaaagcgcaa aaacaaatag aagagtttac gaagaatgga
    24601 tgggacaaac tgccgatttg tatggcaaaa acacagtatt cattttctga tgatccttca
    24661 ctgctaggtg caccggaaga tttcaccatc acgatccgcg aaatcatccc aaaattaggg
    24721 gcaggatttt tagtagcctt gactggagat gttatgacga tgcctggttt gcctaaaaaa
    24781 cctgctgcat tgaatatgga cgtgacaaat gacggagaag tgttgggatt gttttaagct
    24841 tgattataat ctaccatcga aggagctgta ttttgactac agcttctttt tttagacgat
    24901 agggaattaa ttttgtttac actaggatca ttctttttct ttattgtatt ttgagctata
    24961 ctaaagcata ataggagggt atgatgaaaa cgacatttat aagaaaaacc ggtttttacg
    25021 gtatgggtag ctcgattcag ctaagaatca acaatgaaaa acgcagtgtt ctcagtcatg
    25081 aacaaaagat aacgcttgat ctggaagtgc cctttacgat gcaggtcacc ttttattggc
    25141 taaaaagccc cgtttataca gtgaaagaag caaagcctgc ttatgtgatc acaatgaatt
    25201 acttgattct tcaaatctat ccgtttttat ttctagcaac tgggttgggg gccgttttcc
    25261 tacaaaattt atttttcagt ttttttgcag ttattgtgtt gattgcgttc tttttctata
    25321 taaaaaaccg ggcatatttg atcaaggagg aaaattatgg caaactctga tcttttttta
    25381 atcagtttca atcgaatcga aaaatggatg agagacgaga tgggaaatgc acgaaatatg
    25441 ggatttaccg aacttgtcag acgcctagct caaagaaaac aattaatgat cagaaaatac
    25501 gaagatgatc ttttacagct tgctcagtta aggaatgcga tcgtccatga tcggatcgca
    25561 gtcgattttg tcattgcaga gccaaatgat tggacagtca agcgaatcca gctgatcgaa
    25621 caagaactga ttcagcctga aaccgtgctg cctcgatttg caaaacgtgt aacaggtttt
    25681 gaacaagatc ttccgttact tgaactgtta aagatagtag cagaaaaaag gtattcacaa
    25741 tttccgttat acaataaagg aaaatttgta gcattgatta ctttacgtaa tatcgggttt
    25801 tggctagcaa aagaaagcca aaaaggtccg gttgatttaa caaataaacg agcgttggat
    25861 ttgatcatcc aaaacggaaa gtacacaaat tatcattttg ttccagctaa aacacatatt
    25921 tacgaggttg aagcgatgtt tcgtgagcaa gggactttgg aagccgtact tatcactaaa
    25981 gatgggaatc ccgatggtaa tctgctcggg atcgtacgac ctcgtgatat ttataaacaa
    26041 atagaaaagg attgattttt tgttaattat ttattggata ttcagcgctt tgctagtagg
    26101 agtggatcag ttggtcaaat ggtggatcac tgataatcta gcgcttggag agacgaaaaa
    26161 tcttatacca ggtattttgt cactgaatca tattcgaaac actggagccg catggagtat
    26221 gctagaaggg aagatgtggt tctttactat agtgacactc attgcagtcg ttgtgattct
    26281 cacattgatg attaaaaatc gtgaaaaagg taatcgctgg ttcatgattg gattgagttt
    26341 gattctagct ggtgcgattg gcaattttat cgaccgtatt cgtcttggct atgtagtaga
    26401 tatgttccag acagatttta tgagctttcc gatttttaac gtagcagatg tgacacttgt
    26461 atgtggagtg atttgtatgt taatttatat tattttagat gaaaaggatc aaagaaaaaa
    26521 atgacagaac ttaaaacgac gattcaccat gaatctggta ggatcgataa agtattgact
    26581 aatttattca ccgattacag tcgttcgcaa attcagctgt ggctgaaaaa tggagcggtt
    26641 tctgtcaatg gacagccagt caaagcaaat tacaaagtaa aacaaggaga cgagattact
    26701 gtcttgatcc ccgaaccaga aacattagat gtggtcccag aaaatatccc gttgacgatc
    26761 gtttacgaag atgaagatgt agcggtgata aacaaacctc aaggaatggt cgttcatcct
    26821 tcagcaggtc atacgcatgg cacaatggtc aatgcgctga tgtaccatat caaggatctt
    26881 tctacgatca acggcgttat tcgtccaggt attgttcacc gaatcgacaa agatacttcc
    26941 ggactgctaa tggttgccaa aaatgaccag gcacacgaat cattagctca gcaattaaaa
    27001 gataagacct ctttgcgcaa atatgttgct ttagtccatg gagagattcc tcacgaaaaa
    27061 ggaaccatca atgcgccaat cggccgttcg aaggaaaacc ggaaaatgca ggcgataaga
    27121 gaagatggga agtcggctgt cacacatttt actgtattgg aacgtttcga aggatttact
    27181 ctggttgaat taaaactaga aaccggaaga acacaccaga tccgtgtgca tatgaagtat
    27241 atcggttatc cgttagctgg agatccagta tacggaccga agaaaacttt gaaaggcaat
    27301 ggtcagtttc tccatgcgca aatgttaggt tttacgcacc cgactacaaa cgaaaagatg
    27361 atctttgaag cacctcttcc agaagttttt gaaaaaactt tagaaaaatt aagaaaaaac
    27421 attgcatttt aatttagata acggtatagt aattaacaac gaaagcataa aacttaatag
    27481 aattccttta agtttagtcc cgtgaggcta agaagggtgg gagcaaggat acgtatggtg
    27541 cgccataagt agattgctca ttgacatagt atagctatca gtgtatcagt atacgctcag
    27601 tctaaacatt cctcctgtcc tcttagggca ggagtttttt tatgcaaaaa agtaggggga
    27661 atgaaaatgc aagcaaaaga agtagtagat caggtaacca tgaagcgagc attgactcga
    27721 attacttatg agattatcga gagaaaccat agtatccaag atatcgtatt ggtaggtatc
    27781 aaaacacgag gaatctacat tgcatcaaga attgccgaac gcctgaagca gctagaagat
    27841 atcgatattc ctgtaggaga actggatatc actttatacc gagatgacaa aaaagaaaat
    27901 ccagaagaac cggaacttca ttcttctgac atcccagttt ccttagaagg aaaagaagtt
    27961 attttgattg atgatgtttt gtatacagga cgaacgattc gagcagcaat ggacgcagta
    28021 atggattttg gcagaccaag aaaaatttca ttagcagtcc tggtcgatcg tgggcatcga
    28081 gaattgccaa tccgtgcaga ttatgttggg aagaacattc caacatctcg ggcggaagag
    28141 attttagtag agatgcaaga gcttgatgga caagatcgca tcatgatttt gaaagaggaa
    28201 gattagagga gagagaaaca tgtcggagaa aaaaggattt cataatgatg acgtggtgtt
    28261 agatatccac gatcgcccaa ccccaggaca ttgggtagga ttaagtttac aacacttgtt
    28321 cacgatgttc ggagcaaccg tgttagtccc catcttagta ggtatcgatc caggtattgc
    28381 gctagtcagt tcaggactag gtacattggt gtacttattt actactaaag gaaagatacc
    28441 tgcgtactta ggaagcagct tcgctttcat tgcagcgatg cagcttttga tgaaaacaga
    28501 tggttatcca gcgattgcac aaggagcagt cacaactgga ttagtctatt tgattgttgc
    28561 gctgatcatc aaaaagatcg gttcagcatg gctggataaa atcttgcctc caatcgtcgt
    28621 cggtccagtc atcatggtta tcgggttggg acttgcggca aatgcagcaa caaatgctat
    28681 gtataatcag ggcacgtatg atttcaagta tattgctgta gcgctgatca cattaggatt
    28741 aacaattttc ttcaacatgt ggctaagagg ttttctcgga ttgattccga ttttactagg
    28801 tattattagc ggatacatcg tagccttgct gtttggaatc gtggatacta agccaatcgt
    28861 agacgctgcc tggtttgctg ttcctaactt tgagattccc tttgtacaat atgaaccaaa
    28921 gctatatctt ggagcaatca caacaatggc acccatcgct tttgtgacga tgacagagca
    28981 tatcggccat cttatggtat tgaataaact aactaaacgt aatttctttg aagaaccggg
    29041 tcttcataag acgttgacag gtgatggatt agcacaaatc gtggcagcct ttgtcggagg
    29101 tcctcctgta acaagttacg gtgaaaacat tggtgttctg gctatcacac gtgtccatag
    29161 cgtgtttgtg atcggtggag ctgcagtatt tgcagtcatt ttaggattcg taggaaaatt
    29221 aagcgctttg attttaagta tccctggacc agtcatctca ggaatcagtt ttatcttatt
    29281 tggagtaatt gccgcaagtg gaatgaagat tttaatcgag aacaaaatcg attttgataa
    29341 aaagaaaaat ctgttgattg cttcagttat cttagtagta ggaattggcg gactcgtctt
    29401 tgaagtaggc acatttacat tgtcggcaat ggcattagca actgtacttg gcatcatttt
    29461 aaatcttatc ttaccagaaa cttcacgaag cgaagaaaac taaggaggaa acattatgat
    29521 catcaaatca gaacgtatca gcttaaagca tttattgacc gttgaggcac ttactgatca
    29581 agaagttatg ggcttgatcc gcagagggca ggaattcaag caaggtgcta attggactcc
    29641 gcaaaagcgc cagtactttg ctacaaacct ctttttcgaa aatagtactc gcacacataa
    29701 aagcttcgat gtagcggaga aaaaactagg gctagaagtg attgaatttg aagcaagcac
    29761 aagctctgtc caaaaagggg aaacactata cgatacagta ctgacgatgt cagcattagg
    29821 cgtagacgta gcggttatcc gtcatggtga tgagaactat tatgacgaac tgatacaaag
    29881 taaaacaatc cagtgttcga ttatcaatgg aggagacgga agcggacagc atccaaccca
    29941 atgcttgctt gatttgatga ccatttatga agaatttggt cattttgaag gtctgaaagt
    30001 tgccatcgta ggtgatatca ctcactctcg cgtagctaaa tccaatatgc agatgttaaa
    30061 acgtctaggc gcacaagtat tcttctcagg tcctagagaa tggtacgacg atgaattcga
    30121 agtgtacgga cattatatgc cattggatga actactagat caagttgatg tcatgatgat
    30181 gcttcgggtg caacacgaac gccatgatgg aaaagaaagt ttctcaaaag aaggctacca
    30241 tcaggaacat gggttaacag ttgagcgtgc gaagaagttg caaaaacatg caatcattat
    30301 gcacccagca ccagttaacc gtgatgtaga attagctgat tcattagtgg aaggattaca
    30361 gtcgcggatc gttcagcaaa tgagcaatgg agtattcgtc agaatggcaa tcttagaagc
    30421 agtgctaaat ggaaaagcgt aattatagga ggatgaaaaa atgaaaacac tcattaaaaa
    30481 cggtcagatc aatacacgaa aaaatatgac gacaccagca gaaatttgga tagaagatgg
    30541 gcgaatcaaa gcaatcggta caggattctc agaagccgaa tttgatgaag tgtttgatgc
    30601 aaagggacaa ttgatcacac ctggtttagt ggacgtccat gttcatttga gagagccagg
    30661 atttacatac aaggagacga tcgaagctgg gactcgctca gctgctcgtg gcggtttcac
    30721 tactgtatgc gcgatgccga atttaaatcc tgtacctgat acggcagaaa agctgagaca
    30781 agtatatgac atcattagaa aagatgcagt tgtgaaggta ttgcagtatg cgccaatcac
    30841 agaaaatctg cgaagtgaga aattagttga tcaagaagca ctgattgaag aaggtgcttt
    30901 tgctttcaca aatgacggtg taggtgtcca aacagctggg acgatgtatc tggctatgaa
    30961 agaagctgca aaaaacaata aagccctagt agcccataca gaagatgaat ctttattatt
    31021 tggcggcgtt atgcacgctg gcaagaaggc agaagaactt ggtcttcccg ggatcttaag
    31081 cgtaaccgag tcttctcaaa ttgctagaga tctgttgcta gctgaagcaa ctggtgttca
    31141 ttaccatgtt tgccatgttt ctactaaaga aagtgtccgt gtaatcagag acgcaaaaaa
    31201 agcaggaatc cacgtaacag cagaagtttc accccatcat cttattttga tcgacgaaga
    31261 tattcccgaa gatttcggct tttggaaaat gaacccgccg ctaagaggac gggaggacag
    31321 agaagctttg atcgaaggct tgcttgacgg tacgatcgat tgcatcgcta cagaccatgc
    31381 cccccatgga ttagaagaaa aaagccaaag tttcatgaaa tcaccatttg gtatcgtagg
    31441 aagcgaaaca gcgtttcaac tgatctatac tcattttgta gaaactggtc gattcacgtt
    31501 ggaacaagtg atcaattggt tggcagttaa accggctgag atttttggtt tgaatgcagg
    31561 aacgctaacg gttggtgccc ccgctgatgt agcagttttt gatattacac aaacttgcac
    31621 gatcgataaa gaagactttt tatcaaaagg ggagaataca ccttttatcg gctggaaagt
    31681 aaaaggcgaa acacaaatga cttttgtcaa cggaaaactt gtgtggcaga agggagaata
    31741 aaagtatgga acgtttgctt atattagaag atggaacagt tttcgaagga aaggcttttg
    31801 gtgcagatgc tagtgtcttc ggagaaatcg tttttacaac aagtatgact ggttatcaag
    31861 aaactatcac tgatcaaagt tttaacggtc aaatcattac atttacctat cctatggtgg
    31921 gtaattatgg ggtaaataga gatgattatg aatcgatttc tccaacatgc aaaggagtag
    31981 tagtaaaaga acatgcacgt gtagcttcaa actggcgaaa ccaaatgacg ctagattcat
    32041 ttttgaaaca aaaaggaatt cctggaatat caggtatcga tacgcgcgcc cttactcgca
    32101 aaatccgtga agtcggaacg atgaaagcta gtatagttga tgctggagat tccttcgaac
    32161 atgcatttga tcagctgaaa gcaaccgttc tagcaaccaa tcaagtgcag caagtctcaa
    32221 ctaacaaacc ttatccaagt ccaggaacag gtcggaaagt cattgtcatt gacttcggct
    32281 tgaaacatag tattttacga gaattgagta aaagaaactg taacctcaca gtacttccct
    32341 ataacacaga tgccgagacg attttagcgc tatcaccaga cggagtcatg ctgacgaatg
    32401 gacctggtga tccaaaagat gtgccagaag caatcgaaat gatcaaaaaa atccaaggaa
    32461 aagtaccgat ttttggtatt tgtctcggtc accagttatt tgctttagcc aacggtgcag
    32521 atacctataa aatgaaattc ggacatcgag gattaaatca cccggtacga gaagtagcga
    32581 ccggaagaat tgattttact tctcaaaatc atggctatgc agtcgatgaa gccacgattg
    32641 atccagaaaa actcatcgta actcatgtag aagtaaatga cggaacagta gaaggagtca
    32701 aacacagaga ttatcccgct ttcagtgtgc agtttcatcc agatgctgcc cctggtccgc
    32761 atgatgcact acatttattt gatgaattta tggaaatgat ggacgcagga aaggagcagt
    32821 aaacagatgc cgaaacgtac agatatcaag aaaattatgg tgattggatc tggtccgatc
    32881 atcattggtc aagccgcaga atttgactac gcaggaacac aagcctgcct tgccttaaaa
    32941 gaagaaggat atgaagtggt tttagtaaat tctaatcctg cgacgatcat gacggataaa
    33001 gaagtggcag atcaagtata tatcgaaccg atcacacttg aatttgtatc aaggattctt
    33061 cgaaaagagc gtccagatgc tctccttcca actttaggcg gacagacagg cctcaatatg
    33121 gcaatggagc tggcagcatc aggtatttta gaagaattga acatagaact tctaggaaca
    33181 aagctttctg cgatcgatca agcagaagat cgtgatctat tcaaacagtt gatgaaagaa
    33241 ttgaatcagc caatccccga gtctgaaatc gtcaatacag tagaagaagc tgtagatttt
    33301 gcaaataaaa tcggctatcc tgtcatcgtt cgtccagcct ttactttagg cggaactggt
    33361 ggaggtatgt gtgaaaatga agaagaatta cgtatcgtat cagaaaatgg attaagtctt
    33421 tcccccgcta cccaatgttt gattgaacga agtattgcag gttttaaaga aattgaatat
    33481 gaagtgatga gagattcggc tgataatgca atcgttgtct gcaatatgga aaattttgac
    33541 ccggtgggta tccatacagg tgattctatc gtatttgccc ctagtcaaac attgtctgac
    33601 catgaatatc aaatgcttcg tgatgcttct ctttcgatta ttcgagcact taagatcgag
    33661 ggaggctgta atgtccaact cgctcttgat ccacatagtt ttaattatta tgtgattgaa
    33721 gtcaatccgc gtgtttctcg ttcatctgcg ttagcaagta aggcgactgg ctatccgatc
    33781 gctaagctag ctgctaaaat agcgcttgga ttaacattag acgaaatgaa aaatcctgtt
    33841 acaggaacga cttttgcaga atttgaacct gcattagatt atgttgtagc taaaattcct
    33901 cgctggccgt ttgataagtt tgaaaacgga gaacggcttt taggcacgca gatgaaagca
    33961 actggtgaag tgatggctat cggtcggaat atcgaagagt ctttattgaa agctgtgcgt
    34021 tctttagaaa tcggtacaca ccatatcgaa cttcctgagt tggcagacat cacggacgat
    34081 gacctaatcc aaaaagtggt tcgagcacaa gatgatcgcc tgttttatgt agctgaagct
    34141 attagaagag ggtacaccat agaagaatta gcggatttga caaaaatcga tttattcttt
    34201 ttagataaac tgttgcatat cgtagaaatc gaacagcagt tgaaacagca gccgttggat
    34261 gacaagatat tgaaaacagc gaaacaaaac ggttttacag acaaaaaagt ggcggagctg
    34321 tgggaaataa acgaagaagc cgttcgccag atgagaaaag aaaatgattt gattcctgta
    34381 tataagatgg ttgacacttg tgcagcggaa ttcgaatcaa aaacacctta tttttatagt
    34441 tcctatgaat atgaaaatga aagttcacga tcagaaaagc cctcaattct agtactggga
    34501 tctggtccta tccgtatcgg acaaggggtt gaatttgact atgcgactgt gcattctgtc
    34561 aaagcaatcc agcaggcagg atacgaagca atcatcatga acagcaatcc tgaaacagtt
    34621 tcgactgact tttctatttc ggataaatta tattttgagc cattgacctt cgaagatgtg
    34681 atgaatgtga tcgagctaga acagccgata ggagtaatcg ttcaatttgg tggtcagaca
    34741 gcaatcaact tagccgaacc tttgactgct gctggtgtaa aaatcttagg gacaacgatc
    34801 gaagacttgg atcgtgcgga aaatcgtgat ttattcgagc aagcgctgaa agagttggat
    34861 attcctcaac ctttagggga tactgctaca agtaaagaag aagccgtggt tatcgcttcg
    34921 agaattggtt atccagttct tgttcgtcca agctatgtat taggcggacg tgcgatggaa
    34981 atcgttgaaa accagcgaga tttagaagat tacatggaac atgctgtgaa agcatctcca
    35041 gaacatccag tactagtgga ccgttacttg cttggtggtg aatgcgaagt ggatgcgatt
    35101 tgtgacggtg aaacagtact catcccaggg atcatggagc atattgagcg tgctggtgtc
    35161 cattcagggg attcaatggc tgtgtatcca cctcaaacat tatctgaatc gatcaagaaa
    35221 acgatcgaag attacacgat ccgcttagca ttaggtttga attgtgtcgg gatgatgaat
    35281 atccagtttg tcatccatga aaatgaggtt tatgtgatcg aagtcaatcc tcgagcaagt
    35341 cggactgttc cattcttaag taaggtgaca ggaattccaa tggcacaaat tgcgacgaaa
    35401 gctattttgg gagagaaact aaagacactt ggttatgaaa acggacttta tccagaaaca
    35461 aaagaagttc atgttaaagc acctgtcttt tcttttacga aattgcagaa agttgatact
    35521 tacttagggc ctgaaatgaa atcgactgga gaagtaatgg gatctgacag acaattagac
    35581 aaagctttgt acaaggcatt cgaagcttct ggattacact tgcctgaata tggagccgta
    35641 ttatttacga ttgcggacga aacaaaagaa gaagcactag aattagctaa acgttttaca
    35701 gaaattggct atagtctgat aggaacgaaa catacagctg cttatttcga aaaaaatggt
    35761 ctggtcgttg aaacagtagc gaaaatttct gaggaagcag cagaaaaaaa tgtagtcgac
    35821 ttaataagag atggtaaaac acaagtggtt gtcaatacga ttgataaaga tcggggaaat
    35881 gcttctaaag acggtttcat catccgaaga gaagcagttg aacatggtat tccattgttt
    35941 acttctttag acacagcaga tgcgatcgtt cgagtgatgg aatcccgtgc tttttcaact
    36001 aaccctattt aacggcgagc acatgttaag acaaaaagac gaaaaaccgt tgcaggaggg
    36061 gacagatgaa acaagaaatg atggaaatcg ttcagcaaaa acagctggca ccaaagattt
    36121 ttgaaatgac attgaaaggt gatctagtaa aagaaatgca gcaaccagga cagttcttgc
    36181 atattctcgt gccgcgtgca gacttgctat tacgccgacc gattagcttg aatcatatcg
    36241 accacgaaac aaatacttgc cgtatcattt atcgggtgga aggtgagggg actagagtct
    36301 tctctactct ttctgccgga gaccatttag atgtcatggg tcctttaggc aatggttttg
    36361 atttgtccaa tcttcataca ggagacgaag tatttataat cggtggagga atcggtgtcc
    36421 ctcctctata tgagttatca aaacagttag tcgaaaaagg aattaagcca acgcattttt
    36481 taggttttgc cacacatgaa gtcgtttatt atgaaaaaga atttaatcgt ctaggagaaa
    36541 caagaatcgc tactgacgat ggaacatacg gtatgcacgg aaatgtcggt aatttattgt
    36601 taggaacgaa gacggaacct acagctgttt ttgcatgtgg ttcaaacggg ttattgaaga
    36661 cagtggaaca gctgttttct tcgcatcaaa atgtccagtt atcattagaa tcccgcatgg
    36721 catgtgggat aggggcttgc tatgcttgcg tttgtcatac gccggaagat gaaacaggaa
    36781 caaaaagtgt gaaagtttgt gacgaaggac cgatttttaa aataggagag gtaattatat
    36841 gaatgagaat cctttagcgg tcaaacttcc tggcctagaa ttaaaaaatc ctatcatgcc
    36901 ggcaagcggc tgtttcggtt ttggcaaaga atacggaaaa tactatgatt tgaatcagct
    36961 tggatcgatc atggtcaagg caacgactcc aaatgcccgc tttggcaatc ccacgccaag
    37021 agtggcggaa acaccatctg gcatgttgaa tgcaattggt ttgcaaaatc ctggtctaga
    37081 cgttgttatg cataaacttc ttccagaatt agaggagtac aatgagttac ccatcatcgc
    37141 aaatgttgct ggtgcttgtg aagaagacta tgtagaagtt tgtagcaaaa ttggcgaagc
    37201 accaaatgtg aaagcaatcg agctgaacat ttcttgccca aatgtcaaac atggcggaat
    37261 tgcttttgga acagattctg atgttgcttt tcaattaaca caagcagtaa agaaagtttc
    37321 ttctgttcct gtctatgtca aattgtcccc aaatgtaaca gacatcgttc caatcgctca
    37381 ggcaatcgaa gctggagggg cagatgggtt tacgatgatc aatactcttc ttggtatgcg
    37441 gattgacctt aagacaagaa aaccgatttt agccaatcaa acaggtggac tctccggacc
    37501 agcaatcaag cctgtcgcta ttcgtttgat tcaccaagta gcaagtatat ccagccttcc
    37561 aatcattggg atgggcggcg tgcagacagt ggatgatgtt cttgagatgt ttatggctgg
    37621 agcaagtgct gtagctatag ggaccgcgaa tttcaccgat ccgtatatct gtcctaaact
    37681 aattaaagag ctccctgtgc gaatgagcga actaggcatc gaatcgctgg aaagattaat
    37741 caaagaagtg agagaggaac gtgaacgatg aatcaactag accaacgacc aattattgca
    37801 ttagattttt caactaggca agaggtagaa gactttcttc gatttttccc gaaagaagaa
    37861 aaattgtttg tgaaaatcgg aatggaattg ttctaccaag aaggacctga aatcgtacgt
    37921 tatctaaaag aggctggtca taatgtattt ttagatctga aacttcacga tattccgaat
    37981 acggtcgaaa aagcaatgcg gggattggca aagcttggtg ttgatctcac ctgtgttcat
    38041 gccgctggcg gtattcgtat gatggaggcg gcaatgcgag gattagaaga ggggactcca
    38101 gaaggtggaa aacgtccgct acttctagct atcactcagc ttacttctac aagtgaagaa
    38161 gaaatgcatg ccgatcaact aatcgaagtt tctttagaaa aaagtgtgat ccattatgct
    38221 agctgtgcca aaaaagcagg attagatggt gtggtttctt ctgcttggga agtagaagcg
    38281 atcaaagaaa tggcaggaga cgaatttgtt tgcttaactc cgggtataag accagaagga
    38341 acagtcgcag gagatcagac aagagtagtc acaccttctc aggcaaaaaa gatcggctca
    38401 acttttatcg ttataggtcg accaatcact cagtcaacta atccttataa agcatatcaa
    38461 acgattcaaa cagaatggag ctaacctaaa atgaatgttg aacaaagtat cgcaaaagac
    38521 ttattagaaa ttgaagcagt atttttaaat ccaagtaatc cttttacatg ggcaagtgga
    38581 atcaaaagcc caatttactg tgataaccga atcacgatga gctatccaaa agtccgaaaa
    38641 gagatcgcaa aaggattagc aagcaaaatc aaagaagctt accctgaagt acaagtgatt
    38701 gctggaactg caacagcagg aatccctcat gccgcatggg tagcggaaat actggatttg
    38761 ccaatggtct atattcgaag taaggcgaaa gatcatggga aaggtaacca aatcgaagga
    38821 cgtatatttg aaggtcagaa gatggttgtg atcgaggatt tgatttctac aggcggaagt
    38881 gtcttggaag cagctgaagc agcaaaaaga gaaggtgcgg atatccttgg tgttgcggct
    38941 atctttacgt atgagttgcc aaaaggaaaa gcaaatttcg aaaaagccga aatccctctt
    39001 atgacgttga ccaattattc tgtattgatt gaagcagctt tagaagatcg ttatatcgat
    39061 gagcaagaat taactctgct aaaagaatgg aaaaaagatc ctgaaaattg gcagattgaa
    39121 gcgcgcttga tgaagataga gaaatccgat ttgtgtctcc agaatcatca attggaatag
    39181 caaaaatcca aaaactgatt gcgagaagca tcttttcatc aggtatgaaa agaaacttct
    39241 atcgtgatca gtttttgatt tttttaaaat tcatttttct aagactggtt agcagaaaga
    39301 agagaataat ctcttctctt ttttctatta tatatagatt ttctctattt tttggtaagg
    39361 gagctgtttc aaaaatggta aagagcggaa atgttgcgaa tatcagttat ttaatgatat
    39421 gatattcgga aaaagaaatc tcaaagggag gtaactttta tggggttgaa tgaatttatt
    39481 ttaggattga acaatttaga gacgatcaca agggcacctg gattttttaa atatacagag
    39541 catacagttg cagcacattc gtatcgtgta gcatctattg cgcaagttct aggagacatt
    39601 gaagaagcga atggacaacc gatcgattgg aaactgctct atgaaaaatc attgaatcat
    39661 gactacacag agcgttttat tggtgatatc aaaacgccgg tgaaatacgc aagcaaagag
    39721 ttaagaggga tgctgcaaac agttgaagaa aagatgacgg acgaattcat caaccaagag
    39781 attccagctg agttccaaga agtttatcgc cgtcggttgt tcgaaggaaa agatgaaacc
    39841 gtagaaggag aactattagc catttcagat aaggttgacc ttctttatga atcttttgat
    39901 gaaatcatca aaaataaccc ggaaaaagtt tataaggaga tgttcattga ggcagtgact
    39961 acgattaaaa gctttgaaca tcggccttct gttcagtatt tctttaaaga gatatttccg
    40021 gaactattga accaggaatt ttatggaaaa gaggcgtttt tagaaaccgt ctcaacgatt
    40081 gtaaagaaag aagaaaaata aaaatgatat gagatgcagg gggaagtata gttgaaaaag
    40141 aatatggatg tcgaatgggg ctattctgga gacaaaggtc cggaaaattg ggcgagttta
    40201 tgtgattggt ttgcgagagg tgcggaattt ccattgcagt cgcctattca tttgaccaag
    40261 ggggaagaaa ccaaaataga aggagacacg ctttcctttc attatcaaaa gcaacggttt
    40321 actgacaagg aattcaaaaa cacgattcat ttagtacctt ttgaccaact cagttatgta
    40381 gagttcaaga aagaacgcta ttatttgaca gatattcatt tccacttgcc ttctgaacat
    40441 atcatcaacg gaaaacagga agatattgaa tttcattttg ttcatatgaa cagcaaaaaa
    40501 gagaatctag ttgtgggtgt catgtaccag ctaatggtac aggaaggttg gctgtataat
    40561 caagaaaatg gggaaagttg gaacttagaa aaacatgagc attgggtaaa tccggatgtg
    40621 ttctttccgg agcaaaaaag ccatttccat tatattggct cattgacaac tccgcctacc
    40681 agcgggccaa tccaatggtt tgtaatggat catgtaggaa aactgaacca agagtttctt
    40741 ctccccttcg atggtcagta tgctcgaccg aacaaccgac caatccagcc cttgaatgga
    40801 agagaaattt attatttcga ggagtttgag caatgaatat tcagcagatg aaatacgtgg
    40861 tcgctgtagc aaacaatggt agtttcagag aagcggcaaa gaaaatgttt gtctcccagc
    40921 caagtctttc agctggtatc aaagaattag aaaccgagtt agggatcacg ctgtttactc
    40981 gaacgaatcg aggggcatat ttaacagaag aagggcaaga cttcttaaat cgtgccgaga
    41041 aaatattggt ccagcttgat acattagaaa ctcactatct aacgagtgaa aagagagaca
    41101 atttttcgat cgctgcccag cattatgatt tcttaggtcc tttgaccgca cagctactcg
    41161 aggaattcaa agacgagatc aaacaatttc ggatcatgga gacgacaaca gcgagagtga
    41221 tcgaagaagt caaagagcag cacagtgaga tcggcattat ttatatgaat gagcataatc
    41281 aagctggtat cagaagatat ttacaacaag gagagctaag tggccgcagc attggaaaat
    41341 tcaaaacgca tattttttta aggcaaggac atccattagc tgaacacgca gagatcacca
    41401 aagaagaatt gttatcttat cctcaagttc gttttacaca ggatggaaat aatttcccgt
    41461 atttttatga agaccttatc gagatccctg atcaagaaac agtgatttat acgagtgatc
    41521 gaggtacttt gatgaatatc gttcttgaaa ctgatgctta cgcttcagga tctggtattg
    41581 tgattggagg gattcgcgag cacttgcggt taatcccttt ggcagaaggc gaactaaatg
    41641 aattttatat catttattcg gcgaaacgtc cgctcagtga aatcgctcaa agatttatca
    41701 aagagcttac tcggttactg gaccagcaga gctaagaaaa gcagatgaga acgctgttta
    41761 ttgaacaccg ttgatcaaaa aagaggggtt gtgacagaat tttgtcacaa ctcctctttt
    41821 tgtagcaaac aatttataac taattgctgt tgtgggaaag tacgcaaata tgattctaga
    41881 aacactcatt gattacctgt tttgtttcat agctgtaatt ttttcttctt ctggtgtcac
    41941 gataatcgtc ttttggtttt cataaataac ataaccaggc ttggcgccat ttggttttcg
    42001 gatatgcttt acctgtacaa gatcaaccgg cacttgggca gagtatcggt attttgaaaa
    42061 ataggcagct aactcggcag cttctgtaat tgtttcatct gagggcttgt cacttttaat
    42121 gatgacgtgc gaaccgggta tattttttgc atgaagccaa tagtcggttt ttttagctgt
    42181 cttcattgtc agctggtcat tttgaagatt gtttttacca acaagaatga gcgttccgtc
    42241 agaagaaaag tattgatcag gctgagatgg tttcttacgt ttttgttttt ttaagttctt
    42301 ctttttcagg tatccttctg aagtcaattc ttctttaatt gcttcgatat ccattgggcc
    42361 ggcaatttcc agttgagata agacagattc aagatattgg atttcatctt tggcttcttg
    42421 gatttgttcg ccaatcagct taacagcgtt cttcagcttt tggtatcgat ggaaatattt
    42481 ctgtgcattt tgatttggtg tcaatgccgg atctagtgct attttgattg gccgatcttc
    42541 ttcataataa ttaggtaaaa caacttcttt tgcgccgcgt ggtacttgtg tcagaaaagt
    42601 agtaagtagt tcgccatttc tacggtaatt ctcagcgttt tcggattctt tgagtgtttg
    42661 ttcacgtttt ttcaatttat ttttatttcg ttttagttca ttttcgattt ttcgaatcag
    42721 ttctccgcct tgttgttttg cccgatcttt ctctgctttt tcgtgataat agctatctaa
    42781 taattgactg agtgttggat aagctgttac agcagatgcc tgttcggaca atacctgata
    42841 agcaatcggt gtgaaaaaat ctttattttc cgtttcgtaa aaagtaggaa taggctgatg
    42901 gctgatggct gagaaaaatt cgtcccatac aaccatcttt tcattaggac gttccagtaa
    42961 tcttttggtc agctcttgag cgatatcgaa accgattccc tgaaactgac gttggattgc
    43021 ttttggatct agttcagttt gggaaagccg ctgaaagatt ttttctttct cggagctaaa
    43081 tgggttcaag acctcttgtt ttggcggagc aacatattct acaccaggaa gcaaagaacg
    43141 ataagtgttt tgagagcttc caatgtgttt gatcgcatcc agtatttttc cagtttctcg
    43201 attaactaag acgatcgtac tatgacgtcc cattaattca acgatcaata tgatattttg
    43261 tagatcaccc aactcgtttc gtttagcaaa gtggaaatgg atcactcgat cattttcgat
    43321 ttgttcgatg tcttctaaaa tagcgccatc caaatatttt cgaagcatca tcacgaagtt
    43381 aggcggatta tcaggattct gataatcgat ttgggtaatc tgtacacgag cataacttgg
    43441 gtgcgcagat aataataatc gatggttttt gccgcgtgaa cgaatcacca gcacgatttc
    43501 attttcataa ggctgatgga ttttcgagat ccggccagat agtaacgttt cacgcaattc
    43561 gttgaccatc gcgtgggtaa atacaccatc aaaggacatg gttcattcct catttctttt
    43621 cataagtata ttcattataa cgaaaaaaag aagaaaaacc aatttggccg aatagtaaag
    43681 caaaagtcta gaaaaatctt ggactaatta cataaaaaac agtgtttcta tcgttgaaat
    43741 ccaaattttt aggt
//
LOCUS       DAYYOA010000011        42154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000011 DAYYOA010000000
VERSION     DAYYOA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.011"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..865
                     /gene="purC"
                     /locus_tag="ACHBKA_000559"
     CDS             137..865
                     /gene="purC"
                     /locus_tag="ACHBKA_000559"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM9444274.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            865..1119
                     /gene="purS"
                     /locus_tag="ACHBKA_000560"
     CDS             865..1119
                     /gene="purS"
                     /locus_tag="ACHBKA_000560"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM9444275.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1121..1792
                     /gene="purQ"
                     /locus_tag="ACHBKA_000561"
     CDS             1121..1792
                     /gene="purQ"
                     /locus_tag="ACHBKA_000561"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM9444276.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1793..4015
                     /gene="purL"
                     /locus_tag="ACHBKA_000562"
     CDS             1793..4015
                     /gene="purL"
                     /locus_tag="ACHBKA_000562"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM9444277.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4000..5439
                     /gene="purF"
                     /locus_tag="ACHBKA_000563"
     CDS             4000..5439
                     /gene="purF"
                     /locus_tag="ACHBKA_000563"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM9444278.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5462..6514
                     /gene="purM"
                     /locus_tag="ACHBKA_000564"
     CDS             5462..6514
                     /gene="purM"
                     /locus_tag="ACHBKA_000564"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM9444279.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6511..7089
                     /gene="purN"
                     /locus_tag="ACHBKA_000565"
     CDS             6511..7089
                     /gene="purN"
                     /locus_tag="ACHBKA_000565"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM9444280.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7086..8621
                     /gene="purH"
                     /locus_tag="ACHBKA_000566"
     CDS             7086..8621
                     /gene="purH"
                     /locus_tag="ACHBKA_000566"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM9444281.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8637..9911
                     /gene="purD"
                     /locus_tag="ACHBKA_000567"
     CDS             8637..9911
                     /gene="purD"
                     /locus_tag="ACHBKA_000567"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM9444282.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10033..10947
                     /locus_tag="ACHBKA_000568"
     CDS             10033..10947
                     /locus_tag="ACHBKA_000568"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM9444283.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11080..12645)
                     /locus_tag="ACHBKA_000569"
     CDS             complement(11080..12645)
                     /locus_tag="ACHBKA_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444284.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12638..14089)
                     /locus_tag="ACHBKA_000570"
     CDS             complement(12638..14089)
                     /locus_tag="ACHBKA_000570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9444285.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14103..15119)
                     /locus_tag="ACHBKA_000571"
     CDS             complement(14103..15119)
                     /locus_tag="ACHBKA_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9444286.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15339..16427
                     /gene="chvE"
                     /locus_tag="ACHBKA_000572"
     CDS             15339..16427
                     /gene="chvE"
                     /locus_tag="ACHBKA_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9444287.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16481..18007
                     /gene="mmsA"
                     /locus_tag="ACHBKA_000573"
     CDS             16481..18007
                     /gene="mmsA"
                     /locus_tag="ACHBKA_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9444288.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18009..19214
                     /gene="mmsB"
                     /locus_tag="ACHBKA_000574"
     CDS             18009..19214
                     /gene="mmsB"
                     /locus_tag="ACHBKA_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM9444289.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19471..20448
                     /gene="guaC"
                     /locus_tag="ACHBKA_000575"
     CDS             19471..20448
                     /gene="guaC"
                     /locus_tag="ACHBKA_000575"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM9444290.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20712..22136
                     /locus_tag="ACHBKA_000576"
     CDS             20712..22136
                     /locus_tag="ACHBKA_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9444291.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22178..22435)
                     /locus_tag="ACHBKA_000577"
     CDS             complement(22178..22435)
                     /locus_tag="ACHBKA_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444292.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            22597..23706
                     /gene="pdhA"
                     /locus_tag="ACHBKA_000578"
     CDS             22597..23706
                     /gene="pdhA"
                     /locus_tag="ACHBKA_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM9444293.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23709..24686
                     /locus_tag="ACHBKA_000579"
     CDS             23709..24686
                     /locus_tag="ACHBKA_000579"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM9444294.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24711..26354
                     /locus_tag="ACHBKA_000580"
     CDS             24711..26354
                     /locus_tag="ACHBKA_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM9444295.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26362..27768
                     /gene="lpdA"
                     /locus_tag="ACHBKA_000581"
     CDS             26362..27768
                     /gene="lpdA"
                     /locus_tag="ACHBKA_000581"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM9444296.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27930..28205
                     /locus_tag="ACHBKA_000582"
     CDS             27930..28205
                     /locus_tag="ACHBKA_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM9444297.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28211..28978
                     /locus_tag="ACHBKA_000583"
     CDS             28211..28978
                     /locus_tag="ACHBKA_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM9444298.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29146..30975
                     /gene="typA"
                     /locus_tag="ACHBKA_000584"
     CDS             29146..30975
                     /gene="typA"
                     /locus_tag="ACHBKA_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM9444299.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31236..33008
                     /gene="recQ"
                     /locus_tag="ACHBKA_000585"
     CDS             31236..33008
                     /gene="recQ"
                     /locus_tag="ACHBKA_000585"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM9444300.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33122..33424
                     /locus_tag="ACHBKA_000586"
     CDS             33122..33424
                     /locus_tag="ACHBKA_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM9444301.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33427..34590
                     /locus_tag="ACHBKA_000587"
     CDS             33427..34590
                     /locus_tag="ACHBKA_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9444302.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34616..38044
                     /locus_tag="ACHBKA_000588"
     CDS             34616..38044
                     /locus_tag="ACHBKA_000588"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM9444303.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38065..39189
                     /locus_tag="ACHBKA_000589"
     CDS             38065..39189
                     /locus_tag="ACHBKA_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM9444304.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39170..39616
                     /locus_tag="ACHBKA_000590"
     CDS             39170..39616
                     /locus_tag="ACHBKA_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9444305.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39600..39938
                     /locus_tag="ACHBKA_000591"
     CDS             39600..39938
                     /locus_tag="ACHBKA_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM9444306.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40057..40617
                     /gene="rsmD"
                     /locus_tag="ACHBKA_000592"
     CDS             40057..40617
                     /gene="rsmD"
                     /locus_tag="ACHBKA_000592"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM9444307.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40614..41105
                     /gene="coaD"
                     /locus_tag="ACHBKA_000593"
     CDS             40614..41105
                     /gene="coaD"
                     /locus_tag="ACHBKA_000593"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM9444308.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41095..42138
                     /locus_tag="ACHBKA_000594"
     CDS             41095..42138
                     /locus_tag="ACHBKA_000594"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM9444309.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT        13927 a         7224 c         9442 g        11561 t
ORIGIN      
        1 acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata aaaagatgat
       61 tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt aatgtaaata
      121 aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag ccaaaaaaat
      181 atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag caactgcgtt
      241 gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc aaatcacaag
      301 tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg aacaattgtc
      361 taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg ttatccgaaa
      421 tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag aattcatcaa
      481 accaatcatt gaattctact ataaggatga ccagttggat gatccattta tcaatgaaga
      541 gcatatctat ttcttacaac ttgcaaaacc ggaagaaatc agtgagctga aagcccaagc
      601 gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg ttttagtaga
      661 tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg aagtaacacc
      721 ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag acgtttaccg
      781 cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc tatcggaact
      841 aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac ctataaagat
      901 tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat gggatatgaa
      961 acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga tgcagaagaa
     1021 ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt aaatatggaa
     1081 acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg cggtcatcgt
     1141 ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga tcatgggcgc
     1201 tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg gggttttact
     1261 gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc ggttttctac
     1321 aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg gtacatgcaa
     1381 tggattccag atattgacag aagcaggact tttgccaggt gtactgagac gaaatgagtc
     1441 tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg cctttacttc
     1501 agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag gaaattattt
     1561 ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct ttacttataa
     1621 tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag cagggaatgt
     1681 gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat cagaagatgg
     1741 gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat agatgaaaac
     1801 attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg aatggggatt
     1861 gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc ctaattatac
     1921 ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa attctaaacc
     1981 ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg gagaaggcgc
     2041 aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa gtcacaatca
     2101 tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa tcatcagaga
     2161 tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt tcggcgaatt
     2221 agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg gcggatacgg
     2281 caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt gttatgaagg
     2341 aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata tccagaaagg
     2401 gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag gtcgcgacgg
     2461 tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac agcaacgttc
     2521 tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt gtttggaatt
     2581 aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag caggacttgt
     2641 ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga cattagatga
     2701 agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg aatcacaaga
     2761 acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac tatttcagaa
     2821 gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat atcggctata
     2881 tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag atgcaccagt
     2941 ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt tagctcctta
     3001 tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc aaccgacgat
     3061 tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca atacagttgt
     3121 ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag cgttagctat
     3181 gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag gacaaatagc
     3241 agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg cgatcaccga
     3301 ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat cgacttcagc
     3361 tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag gaaatgtctc
     3421 tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg gtatggtcgg
     3481 attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag gggatctaat
     3541 ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa agatgcagct
     3601 tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga gaaaccaaga
     3661 gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact gttcagaagg
     3721 tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga tatctgtaca
     3781 gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg tgttgacagt
     3841 cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg ttttagcagg
     3901 taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga tcaaaataga
     3961 aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga agtgaagagt
     4021 ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc tagtgtggca
     4081 tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat cgtgtccaat
     4141 gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt attcaaagat
     4201 caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg ttatgcaaca
     4261 gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca tgatcagtcg
     4321 atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa agagttagag
     4381 gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca tttgatccga
     4441 cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga agtcaaaggt
     4501 ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga cccaaatggt
     4561 tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc ctcggaaact
     4621 tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg tgagatagtg
     4681 atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca gctttctatt
     4741 tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg gatcaatgtg
     4801 catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt aaaagcagat
     4861 atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc ggaagctagt
     4921 ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac atttatccag
     4981 ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt acgaggagta
     5041 gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac gacaagcaga
     5101 cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag gattgcttct
     5161 cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa agaactcatt
     5221 gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc tttaggtttt
     5281 ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc gtattcagga
     5341 ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta tgaagaaaaa
     5401 tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa gaagggagta
     5461 tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag ctggatatga
     5521 agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta tgatgggcgc
     5581 tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag agccagtcct
     5641 tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag cagacaaaca
     5701 cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag cacaaggcgc
     5761 agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt tacgtttgga
     5821 aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc tgattggcgg
     5881 agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag ccggatttgc
     5941 tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc caggagatgt
     6001 gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg ttcgcaaaat
     6061 tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga aaaaaacact
     6121 tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc cgctgatcaa
     6181 agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag aaaatatccc
     6241 gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc cgattcttcc
     6301 aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt atgaaatctt
     6361 caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag aaatagtaca
     6421 cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg ccaaagaaaa
     6481 acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc ttctggaaac
     6541 ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga atcttcgatt
     6601 gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac tgcgttgagt
     6661 gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta tgaagaagca
     6721 attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg atatatgcga
     6781 atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat ccatccgtct
     6841 cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc tggagtaaaa
     6901 gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc aatcatccgg
     6961 caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga aaaaatccat
     7021 agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa tggaggctat
     7081 ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta gcattcgccc
     7141 aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag catgcgttgg
     7201 ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct gaaatgatgg
     7261 atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga agacgcgatc
     7321 tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac tttgtttgtg
     7381 tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata gcagaagcta
     7441 ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa aaccatgaat
     7501 ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa ttacgtcagt
     7561 atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt cgtcatactg
     7621 cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa aaagaaccgg
     7681 aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa aatagccatc
     7741 aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca gcagcaaaac
     7801 aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct gcgctacgga
     7861 tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat ccttgtggta
     7921 ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca gatcctgttt
     7981 caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca gcagaacaga
     8041 tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa gctttggaat
     8101 tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag gaagaaaatc
     8161 aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat caagatctgg
     8221 caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact ccagcagaag
     8281 caaaagcatt agcgtttgct tggagagctg tcaaacatgt gaaatctaat gcgatcgtac
     8341 tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga gtcggttcag
     8401 taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta gctagcgatg
     8461 cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata aaagcaatca
     8521 ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat aaatacgatc
     8581 tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc ggataaatga
     8641 acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa cttttagcca
     8701 gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca aaagatggta
     8761 tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt gttaagaaag
     8821 aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg atcgtcgatg
     8881 attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct ctgatcgaag
     8941 gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca gctaaatcca
     9001 gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt gcgccaatcg
     9061 tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc acagtgaagg
     9121 aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat agtagcaaaa
     9181 aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg tttgtccgta
     9241 ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt gataacgata
     9301 aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt tcaaaaaaga
     9361 tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg aaagaactag
     9421 gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa ggaccaaaag
     9481 tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt tctcggttga
     9541 agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta gagttaaagt
     9601 ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac cccgaagaat
     9661 atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt tactatgctg
     9721 gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat ttagtagaag
     9781 catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta atggataaaa
     9841 cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat agcgaactga
     9901 tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa tcggaagcgc
     9961 cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt atataaataa
    10021 ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc agctgcagtg
    10081 atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat tgagacagct
    10141 tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg tgaatgtgaa
    10201 gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct ttttattcaa
    10261 acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca tatcattgaa
    10321 tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt acttcttcat
    10381 cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga attggaacgg
    10441 tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat cgaattgctg
    10501 aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt gaaacatact
    10561 ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat cgatcatgac
    10621 ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg gtcaccatac
    10681 cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga attaaaccaa
    10741 acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc ttctgcttgg
    10801 attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt agggcgaatc
    10861 aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta cgaattgtac
    10921 cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa gggctataaa
    10981 ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg tcttttttta
    11041 ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt tgtttttaag
    11101 gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa ataaataact
    11161 gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac gatagtctga
    11221 ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat cgatgaatgt
    11281 ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg caacttgttg
    11341 agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa aatccaatgc
    11401 atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag cagttaagaa
    11461 agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga ttgctaagcg
    11521 ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt tcaggcagac
    11581 atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag ttggtgtgcc
    11641 ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata atgaagacaa
    11701 atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt cttgaatcaa
    11761 atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta tcatctgcgt
    11821 gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa tggcgacaga
    11881 aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta cctgataact
    11941 ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga atcgagagga
    12001 aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat aatcactgag
    12061 atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata cggtcatttt
    12121 tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac tgatcgtaag
    12181 tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa caagatcaga
    12241 agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt ctattgcttg
    12301 tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta cctggattgc
    12361 tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag tccaaggaag
    12421 tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt ggatatcagt
    12481 cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac catctccagc
    12541 ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt cccgcaagtt
    12601 attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg tttcctccaa
    12661 ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg tatttgctgt
    12721 caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg tttacgttgt
    12781 aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct ttacgtattt
    12841 ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat tggatcatct
    12901 catcaatttt gcatgcggta atacgaataa atcctttgtg acggacgtac ttcgttccat
    12961 ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa acaggaaaaa
    13021 gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt tttaagatga
    13081 ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct tgtcctttgc
    13141 ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct tcttcaaagc
    13201 agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa tggggagtaa
    13261 tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc aaagcgttct
    13321 cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta agtgtttgta
    13381 attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa agttgttctg
    13441 acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag cgacgattct
    13501 ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt aatagaatga
    13561 gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca cgaacaaaat
    13621 cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg atctcttcat
    13681 tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga taatcttcca
    13741 gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt gtatttgtat
    13801 tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc tcattgaact
    13861 catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact ttttgatcaa
    13921 ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga taaatcaaaa
    13981 gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat agaagcatgg
    14041 ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg atacttctcc
    14101 agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac agtctcggaa
    14161 ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata acgagtaacg
    14221 gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt gacgattcct
    14281 gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat caaatcttct
    14341 ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat ttcatcacta
    14401 tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg tttcatctgg
    14461 tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt catcggaggt
    14521 ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga ttccaaaaat
    14581 ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc taggattctg
    14641 atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc ccgatttcct
    14701 tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt cgtttgaata
    14761 tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa tacagagtcc
    14821 cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc aatgaacgtc
    14881 cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag aacttggtaa
    14941 tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca gctactctcc
    15001 gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt attaggtgta
    15061 caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac acgtttcact
    15121 ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg aaaatcaaag
    15181 tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga caaaaaaagt
    15241 acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct ttctttatta
    15301 gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa tttagaaaga
    15361 ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt ggcaattcag
    15421 gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa tcagcagaaa
    15481 gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt tataaaacag
    15541 atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa aatatgatta
    15601 caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta actgatgtgt
    15661 tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta ctgatgaatt
    15721 cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta tcgcaagcag
    15781 cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg attgaattat
    15841 ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg atgtctgttt
    15901 tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg agtttcagtc
    15961 aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat aatctgttaa
    16021 gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat ccaataagtt
    16081 taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa cctttacctg
    16141 tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc ggggaacaaa
    16201 cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa atgatcaaag
    16261 caatcagtga tggagaagag gttccggtca atgatactga aacttatgat aatggggtga
    16321 aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat tatcaagcag
    16381 agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag gaaccaaaca
    16441 aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt gtattttaaa
    16501 aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg tgaatctgat
    16561 gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa aatcaacatt
    16621 gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa tcttttatga
    16681 tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca tcgtcatcat
    16741 tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt ttctcggaaa
    16801 tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa catatgaact
    16861 gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga ttggcgtagg
    16921 gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc tgttgatttt
    16981 ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga gattgatccg
    17041 agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga atgaaatcgt
    17101 ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga cactagaaaa
    17161 tgaaatgatc agcgaagagc atattattcg cgggatggtg ggcagagatt tagttgatcg
    17221 ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag actggacggt
    17281 ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc atttgaaaaa
    17341 aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat tcgctatgag
    17401 tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg aaggggcaga
    17461 gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg tctcggagga
    17521 ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga cgctgagcag
    17581 tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa ctgaggcaga
    17641 aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg tcagtagtct
    17701 tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac aacccgatat
    17761 tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg agatctacac
    17821 gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt ctgaacttcc
    17881 tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta ttacaggaga
    17941 agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga aagaagaggt
    18001 gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa ctaaatttga
    18061 aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta gcgggactac
    18121 ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat ataactaacc
    18181 ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta gttgtgctct
    18241 taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca attgcgggca
    18301 tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt cttgcagtag
    18361 gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata ccggcgttta
    18421 tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt ctaggaggcc
    18481 aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat ttaccagact
    18541 taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc atcacgatcg
    18601 tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta tttgaagtgg
    18661 aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta ctaggaataa
    18721 cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt ggtttgatcg
    18781 ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt tatgcgacgg
    18841 gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc acgttctggg
    18901 tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct gctcgattga
    18961 atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg gcagtatatt
    19021 ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt gtcggtggct
    19081 tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta gactggcagc
    19141 aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat aataagaaaa
    19201 aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa gacatttgtc
    19261 acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga ctaaaaataa
    19321 tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt ttgcttaatt
    19381 gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg ttcgtgtttt
    19441 gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga agatattcaa
    19501 ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac ggtcacatta
    19561 ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat tattgatgaa
    19621 acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg gtttgatgaa
    19681 gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac ctcgatcagt
    19741 gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga atcgctaaat
    19801 cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat tgatatgatt
    19861 cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt tggtacaccg
    19921 gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg gattggtcca
    19981 ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg gcaactggca
    20041 gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg tgggatcaga
    20101 acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat gattggttca
    20161 ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg aatcgtgtat
    20221 aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa tgttgaagga
    20281 aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga aatgcaacaa
    20341 gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat tcgaaaagtt
    20401 gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa aataaataaa
    20461 atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc caaacaaaaa
    20521 tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc ttttgaacat
    20581 cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat gttttgcaaa
    20641 aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata gacaatagaa
    20701 aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat cgtcaaatta
    20761 tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt caacttgaat
    20821 cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc catgcattgg
    20881 aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg tttgttaatc
    20941 agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg atctctcctc
    21001 gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag atccaaaaaa
    21061 catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg aattttgagt
    21121 ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat cttttaggct
    21181 atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa ttggcatttt
    21241 cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg attttacaaa
    21301 cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt taccgattac
    21361 ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa gaagggatcg
    21421 acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg cttatcaacc
    21481 cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa aagctagtag
    21541 aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg gatctttcat
    21601 ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat gtgttatacg
    21661 tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg ataggacctg
    21721 agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg atgaatatcc
    21781 ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa atgcaagatg
    21841 taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat tcacaatcgt
    21901 ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg gatgggaaaa
    21961 aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt gcaccaagtc
    22021 tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg gatgaaaacc
    22081 aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca gaataatttc
    22141 acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc tgtttctttt
    22201 ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt gtacgtccgt
    22261 tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca acgtttttaa
    22321 atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat gaaaaataaa
    22381 acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt ggcataattt
    22441 cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca tgataattta
    22501 ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg cagaatgaag
    22561 agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa agcaccgatc
    22621 gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca agcacttgat
    22681 caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga tgaactcgtt
    22741 gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac agcgctgaac
    22801 cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag tcagctggct
    22861 agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga tgtacctcaa
    22921 ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg acatgctgca
    22981 ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat cggtgcacaa
    23041 tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa aaatgtcgtc
    23101 ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc aatcaatttc
    23161 gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta tgcgatctct
    23221 acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt cgcagctggg
    23281 attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt atctaaaatg
    23341 gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact gacttatcgt
    23401 tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa agacacagat
    23461 gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac ggaaaaagga
    23521 ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga aatcaaagca
    23581 gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt gaaaaacatg
    23641 ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc gaaggagtcg
    23701 aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta gcacttgaac
    23761 tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac ggaggagttt
    23821 tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc gatactcctt
    23881 tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga tttcgtccag
    23941 ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta gtagctcaaa
    24001 tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca attcgttcgc
    24061 catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa ggtttgatcg
    24121 ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca aaaggactat
    24181 tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg aaactatatc
    24241 gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat aaagcagcag
    24301 ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg cgtgaagcaa
    24361 tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt gatcttcgta
    24421 cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact ggtcgcgtag
    24481 ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt tctgagatct
    24541 cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca cctgatacag
    24601 tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat atcgaagaca
    24661 aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc atggcttatc
    24721 aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg
    24781 tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa aatgacaaat
    24841 cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt gttccagaag
    24901 gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga cacgaagaca
    24961 atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg gctgctgaac
    25021 ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa tttaagttac
    25081 cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag
    25141 atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa
    25201 ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc actgtagcaa
    25261 atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca ccgtcaacta
    25321 gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca gcaagtgtcg
    25381 ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt caatttgccc
    25441 gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc agagtaacaa
    25501 aagaggacat cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg aaatcagaag
    25561 cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa ccagctgcac
    25621 cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca atgacgccaa
    25681 cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca cacgtgacat
    25741 tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt aaagaagttg
    25801 ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct ttaacagcga
    25861 ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa gaaatcgttt
    25921 ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta tatgtaccaa
    25981 atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc aatgagaaag
    26041 caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga acaattacga
    26101 tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac tatccagaag
    26161 tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct gaaggcgaaa
    26221 tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc gtagatggag
    26281 caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca gagctattaa
    26341 tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt agatacagtt
    26401 gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga aatgggacaa
    26461 aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt cggctgtatt
    26521 ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga ttctgaaatg
    26581 tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga atggaaagaa
    26641 aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa acataaggtt
    26701 gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt cattcatcca
    26761 gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc ccgtccaatc
    26821 gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg attagcattg
    26881 aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc cgaattgggc
    26941 ggagcgtacg caaacttagg agcagaagta acgattcttg aaggctcacc acaaatttta
    27001 ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa aaaaggcgta
    27061 acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag cgtgacagtg
    27121 aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat ggtcactgta
    27181 ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga agtaggagaa
    27241 cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat ctatgccatc
    27301 ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc aaaaatcgct
    27361 gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc ggcagtagcc
    27421 ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa agatgctggt
    27481 atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt atcgcttggt
    27541 aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct tattggcgcg
    27601 caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc aatcgaatca
    27661 ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct tggcgaaatc
    27721 gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt tgaataatac
    27781 aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc aataatatta
    27841 ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta tgctacactg
    27901 aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca cttgatttag
    27961 attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga
    28021 atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt aaaactgttg
    28081 tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct tccggttatt
    28141 ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg agaatgaacg
    28201 ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag aagctggtga
    28261 gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc gaacggatct
    28321 agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc attcctttga
    28381 tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg atctcgatgg
    28441 aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg aaggggaaaa
    28501 tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt tcatttatga
    28561 cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata aaaatgatga
    28621 gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg tgaatagtta
    28681 tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca tggggacacg
    28741 tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc acattgctta
    28801 tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg aagagtttgg
    28861 tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg aatactattt
    28921 agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa
    28981 aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat tttctcgaaa
    29041 aaatgaaaac attttactat atttcatgaa aaaagtctgc tataattagc agtcgagtaa
    29101 aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa ttatagaaac
    29161 gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac attagttgat
    29221 gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga acgtgcgatg
    29281 gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa cacagctgta
    29341 gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga cttcggtggc
    29401 gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa
    29461 ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca tatcacacca
    29521 atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa
    29581 gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt tcctgtgatt
    29641 tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga tcaagaacca
    29701 accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt cgataacagc
    29761 gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt aggtcgtatc
    29821 ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc attaattaaa
    29881 ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt tggactaaaa
    29941 cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg tatggaagat
    30001 atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc tatccttcac
    30061 attgacgaac caacattgca aatgactttc ttagttaata attcaccatt tgccggtcga
    30121 gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca
    30181 gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt ttctggtcgt
    30241 ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata cgaattgcaa
    30301 gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa
    30361 cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc cttgagctta
    30421 cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg tttgacattc
    30481 ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat gacacgtggt
    30541 tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc
    30601 ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac ttattctatc
    30661 atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt atatgaagga
    30721 atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac gaaagcaaaa
    30781 caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa
    30841 caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg cgaagtaaca
    30901 ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct
    30961 aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta taaaaaatag
    31021 ctggtttctt tttttgtcca actcttttct gtggagaagt catctttgtc aatggacata
    31081 aattcagaaa atacgccgaa agacgtttaa aagactttta aaaaacttat ttctagagag
    31141 tttttgtttc ataataagat aatgtttcac gaaaggagat caacttgtcc ttcgaagaaa
    31201 atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa cgacttatta aaacaatact
    31261 ttggttatga tgagtttaga cctggtcaaa aagaaattat ccagaaagtc attgatcagg
    31321 aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc aatctgttac cagcttccag
    31381 ctttactatt agatgggctt actgtggttg tgtccccttt aatctctttg atgaaggatc
    31441 aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc tcgttagatg
    31501 gttacgaaac agctagacgc ttccaagaaa tcgatagaca gcagtaccgt ttactttata
    31561 tcgctcctga acgttttatc atgccggatt ttatccaagc aatgaagcga tggaacgtcc
    31621 gtatgatcgc tattgacgag gcacattgta tctctcagtg ggggcatgat ttccgcccaa
    31681 gttatttaca gatggcgaac cagctagatc aactgccaaa tcgtcctgta attgttgctt
    31741 tgacagctac agcgactgtg caagtagctg ctgacataaa aagactttta aaaattccag
    31801 aaaacaacca tattcagact ggttttgaac gagaaaatct acgatttcaa gtaattaaag
    31861 atcaaaaaaa agaacaatat ctaatcgaat atttaaagat caacaaaaat caatctggta
    31921 tcatttatgc agcaacgaga aaagaagtag accgtctcta tcatttgctt aaaaaattcg
    31981 atttttccgt gggtagatat catggtggtc taaatgagaa tgagcgaaca gaaatgcaag
    32041 aagccttttt atacgatcgc ttgcagctga ttgtagcgac aaatgcattt ggtatgggta
    32101 taaataaaag caatgtccgt tttgttatcc attatcaaat tcctggctct ctagaagcat
    32161 actatcagga agctggtcgg gcgggaagag atggtctttc cagtgaagcc atcctgcttt
    32221 tctctcctca agatatccaa gtccaaaagt tttttatcca gcagtcccaa agagaagaag
    32281 ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac agaatacgtg tatattgagt
    32341 cctgtcttca gcaatacatc ctaaactatt ttggcgagac aagcagtccg tgcaaccgtt
    32401 gtggtaactg tttagatgac agagaaatag tagaagtaac gacagaagca cagatggttc
    32461 tttcttgctt aaaacgtatg ggtgagaact atggtaaaca aatgctgatg aaagtcttag
    32521 caggatcgaa agaacaaaag ctgcaagctt taggttttgg acatttatct acctatggac
    32581 tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg atatcgaatg
    32641 gctatcttct gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa agagggatac
    32701 aagtattgaa aggacaagaa tctgtttatc gaaaggaacc caagaaagtc cagcagctat
    32761 cggatgaaga aaccgataca ctatttgaag tgctgcggga actgcgaacg gatctagcat
    32821 cagaagcagg cgttcccccg tatgtcgtat tttcagattc cacattgaaa gaaatgagcc
    32881 gaatacgtcc atcaagcaga ttggagatgc ttcagataaa aggagttggc caaagcaagc
    32941 ttgataaata tggcgaagcc tttttatctc ggataaaaaa tgcggactct aacgttctgg
    33001 ataagtagaa agaacgaata cgtaaaaatg aataaaaaca actggttaaa tctgtttttt
    33061 ttgctcctta tgaaaatcaa atatgttata attaaggaat tagaatggga ggtaagcaca
    33121 gatggattct ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag cagaacggat
    33181 caaacgatta atccgaaatc aaaacaacag tctctgtatc tcacaatgca aagccttcga
    33241 agaagtggta gatacacaga tgtacggctt ttcccggcaa gtcggttttg ctgtccgtat
    33301 aggagtaatc ggaaagaacg agggccagct gatgttaagt gagctagaac gagaattgaa
    33361 tcagttatat agtgaggttt accaagaaat ttatgataaa aaagaagttg gcaaggagga
    33421 atagacttgc ctaagaaagt gaaaaaaagg catctattag actatagcat cttgattccc
    33481 tatttaattt tatgtgtatt gggattgatc atggtatata gttctacgtc gtatctatta
    33541 ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc agagtatttt ttgggtgctt
    33601 agcttgattg ctatcgcact actttataaa atgaaaacag atgttttgaa aaaccagcgt
    33661 ttgatcatgg cagccatcgc agttttgacg attttgctgc tgattgttgt gttttttggt
    33721 aaggagatca atggggcgaa aggttggctg caaattgcag gattctcgat ccaacctgcc
    33781 gaatacctaa aaatcatttc tatctggtac ctgtctctga ctttatctaa aagacaaaac
    33841 agtgtacaaa aagattttct tgggactgtg aaacgtccac ttgcaatggt tatcggactt
    33901 actgcattag tcgctattct tcccgatttt ggtaatgcgg cagttatctt tttgattatt
    33961 ttggttctgc ttttagccag cggagtcaat tatgtgtata ctttgattgt aggagtagga
    34021 ggcttttgtc taagtacttt tacgatctgg ctgatcaata tcacaaacgg aaagatctta
    34081 cccggacgtt tacagtatat ctataatcga tttgctattt accaaaatcc tttctcagac
    34141 gaattaaata aagggcatca actagtcaac ggctactatg ccatgttcaa cggaggacta
    34201 tttggcaggg gattaggtaa cagtatccag aaaaaaggat ttttacaaga agcacaaacc
    34261 gattttattt atgcaatcgt agtggaagaa ctgggagtga ttatggggat tttgatatta
    34321 gcgttgctat tctttatgat tgtgcgcatt gtccttgtag gtatccgttc aaaagatccg
    34381 ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt atttgtcaat
    34441 cttggaggaa ttactggcgt tattcctttg acagggatca cgttcccatt tttaagtcaa
    34501 ggaggctcca gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa catcagtgcg
    34561 gatgaaaaac gcaagagctt aggtttataa taaaaaaaga tgtaggagtg gttcgatgaa
    34621 aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg cttgtacaga
    34681 attaggaatt aaaactgttg gaatctatgc agaagaagat gaatattccg tacaccgttt
    34741 caaagcggat gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg cgtatctaga
    34801 tattgaaggg atcatttcta tcgcgaaaga atgcggagct gacgctattc atccgggtta
    34861 cggtcttttg tcggaaaact tgaattttgc gcaacggtgc gaagaagaag gcatcatttt
    34921 tgttggaccc aaactgcatc atttagatat ttttggagat aaaatcaaag caaaagctgc
    34981 agctatcgaa gcggggattg cttcgattcc aggcacagat ggaccaatcg cttctataga
    35041 cgatgccttg gagtttgcga aacaatacgg ttatccgatt atgatcaagg cagctctagg
    35101 cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag agggctacga
    35161 acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg ttgaaaaata
    35221 catcgctaat cctaagcata tcgaagtaca gattctcggg gatacgcatg gaaacgtcat
    35281 ccatcttttt gaaagagatt gttctgttca gcgccgtcac caaaaagtag tagaagtagc
    35341 gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag ctgtccaatt
    35401 gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg aaggagatga
    35461 tttttacttt atcgaagtca accctcgcgt ccaagtggaa catacgatca cagaaatgat
    35521 tacagatatt gatatcgtaa caacgcaact gttgattgct caaggtttgg atctgcataa
    35581 agaaatcgga ttgcctcagc aagaaggaat taaattaaat ggttcagcca ttcaatgccg
    35641 aatcactaca gaagacccgt tgaataactt tttaccagac acagggaaaa tcgatacgta
    35701 tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg cgggctatgt
    35761 agtgacaccg tattttgatt cattgctagt aaaagtatgt acgcatggtg ctacctttga
    35821 aacagccatt cagaaaatgg aacgttgctt gaaagaattt agaatccgtg gagtgaaaac
    35881 aaacattcca tttatgctca atgtcatcac acatcctgaa ttccaatcag gaaatgcaaa
    35941 aacaaccttc attgatagta cagcaacatt atttgaattt cctcgactac gagaccgcgg
    36001 aaacaaaacg atgaaatata tcggtgaaat tactgtgaat ggttttccag gtatcgaaag
    36061 tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa gatctaatca caagaactga
    36121 ttacgtaacg gctaagaatg tattagatgc gaagggtgcc gatgctttgg tcgaatggat
    36181 caaaggacag gaaaatttgt tgttgacgga tacgacattt agagacgccc atcaaagtct
    36241 gctagcaact cgtgttcgga caaaggattt caagcagatt gcacgtttga ctggagaagg
    36301 tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca acatttgatg tggcttaccg
    36361 ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa tgcctaatac
    36421 gttgctgcaa atgcttttca gaggttcaaa tgccgttggg tattctaatt atcctgacaa
    36481 tgtattggtg gaatttgtca aagaagcagc agcgcaagga atcgatgtat ttcgaatctt
    36541 tgatagtttg aactggactc cacaaatgga aaaaagtatc caagctgtac gggatactgg
    36601 taaaatcgca gaggcagcta tttgctatac tggcgatatc aacgatccaa gtcgggcaaa
    36661 atacaatgtt cagtattata aagacatggc aaaggaacta gaacaactgg gtgcgcatat
    36721 cattgcaatc aaagatatgg ctgggctgct taaaccgcag gcagcttatc gtttgattag
    36781 tgaattgaaa gaaacgacag atctgccaat ccatctgcat actcatgata ccagcgggaa
    36841 tggaatcatc acttattctg ctgcttcaaa agcaggagta gacatcgttg acgtggcaat
    36901 gagtgcaatg agtgggaata ctagtcagcc tagcatgagc agcttgtatt atgcactggt
    36961 caatggtcca cggctgccag aaatcacgat tgaaaatgca cagaaattaa accattattg
    37021 ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc ctgaaacaga
    37081 agtatatatg catgaaatgc caggaggaca gtattcgaat ctacagcaac aggcaaaagc
    37141 tgtcggatta ggtcatcgct gggatgaaat caaacaaatg tatcatactg tcaacttgat
    37201 gtttggcgat atcgttaaag taactccatc atctaaagta gttggagata tggcactatt
    37261 tatggtccaa aatgatctta cagaagaaga tatttatgaa aaaggcgaaa cactaagttt
    37321 ccctgaatct gttgtgactt tcttccaagg agaactaggg caacccgttg gcggatttcc
    37381 taaaaaattg caaaagatta ttcttaaagg cagacctgca ttgaatgaac gtccaggtct
    37441 tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa aaattggcta
    37501 tgaaccaaaa caagacgaag tattaagcta tttgatgtat ccgcaagtct ttttggacta
    37561 tcaaaaagca tataatcaat ttggcgatgt tacattactg gacacaccaa catttttcca
    37621 aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa cgcttatcat
    37681 tcgtttagat gaaatcggag aacccgatat cgaagggaat cgcgtactgt tcttcaattt
    37741 gaatggacaa agacgtgaaa tcactatcaa agataattcg atcatcagta cggtccaaac
    37801 ccgattgaaa gctgaaccga caaaccgaga acaaattgga gcaacgatgt ctggttctgt
    37861 tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt tgatgattac
    37921 tgaagcgatg aaaatggaaa cggccatcga agcacgtttt gacggagaag tcgcacatgt
    37981 atatgtgtct tcaggagaca ctatttcgtc tggtgacctt ttgattgaag tgactgagaa
    38041 ataataagcg aaaggtgggg aaacatgaaa agactgggtt tgttttggct gattctgcta
    38101 ttagttattg tggcattcta catggaacca gtcttgtttc cgccaaaact tgaagtgaag
    38161 gaaaactctg atcaacaaat atcaaaccaa atgacaacgt ggaaatacca ggagcttaaa
    38221 gcaactggtt tttctactta tatcggaaaa ccgatcgatc agctggaaaa ggattttggc
    38281 caagcctatg atcgattaac tagcggattt ggttttgaaa caagatatta tgaaaataaa
    38341 gctagtcatc tcacatttga agcgaacatc caagacagca gagtcacaac tgttaaagta
    38401 ctgcaaacag ggcctgctga tatcgcgccg ttttatttag ggatgacgat gcaagatctt
    38461 acgaaaatca ctactatcta tactaatttc acttttgagt accatgatac agaagttgga
    38521 atcgaactaa tggaagagga tatgaattat cgtccgctaa ttgcttttga taacgacact
    38581 tttgctattt tattttttga tcaaacaaca aacggcctat tttcagttgt gtatttagat
    38641 aaagatagtc ttttgaaact attgccatat caagtgtttg gtgaaggcct tccacattat
    38701 caagcagata aatcggctga ttgggaatca atcaatcgcg ttaaagaacg aaaaagtacg
    38761 actttattaa atatgcttag aagagaagac gaacttccca tatacaatca aacacttgcc
    38821 tttcaaaacc acagtcagct gctactacat gattttttga caaggcctga ggaaatattg
    38881 tcagaggaac gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac gacagtgaaa
    38941 ttctcactga cgaatgatga attggagaag ttaatcagta aacaaaaatt agaaaatact
    39001 agtgggctat ttattcatcc ggcgattgac gctacttttt cgtttttata ttggtacagc
    39061 gatccttaca accatgaccg tttcatggac ccgggaacgg attctgtagg cattgctttt
    39121 tctaaagaaa atatgttagt cttattgcaa gaagaaaacg gagaaagtag tgatagcagt
    39181 gatcgttaac gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg ctgaaatgat
    39241 aaaaaagagt aaaacgatgg cttcttatct tgaacataaa cagtcaatgg aagaatctgc
    39301 aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa gaagcatttg aaaatgtagc
    39361 ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc gaaaagcaaa
    39421 aagacagctt gatatggatg agcatgttgc agctttccga tatagtgaaa cacagctgca
    39481 aagtctgttg gatcaaattg tccaagtact tgccgaagaa gtttctaatg aaatcaaagt
    39541 agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag gtaattgtca
    39601 tgcaattaga caatagtgaa tttgaaataa caaaacgacg cgggctgatc gtatgggtct
    39661 acagcttaaa acaattaaag aatctgaaaa agttcggctt gttgcattat gtctcaagaa
    39721 gaatgaaata tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca gaagaacgaa
    39781 tccgtaaatt acattttgtt cgaaacgtag aacgttctta ccgaccggat atcgaaatga
    39841 attttgccga aaaaatagga aacaaaacag tattaaaaga cgaaggtttc gaaattgaag
    39901 agctaaatac agaaatccga ttagctgaac atctttaaaa atctcttatg agcagttttt
    39961 aaataaaaaa taacttggat aacgatcgat ttttgatcat tgtccaagta tttttttggt
    40021 atactagtac cagtaccatt tctaaggagt gagtagatgc ggataatatc aggaaattat
    40081 ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca gaccaactac agataaagta
    40141 aaagaatcca tattcaatat gattggacca tattttgatg gagagacagt attggatctg
    40201 tttgcaggaa gcggtggttt agctatcgaa gcagtatcta gaggctgcag tcatgcggta
    40261 tgcgtagaca aaaattacca agcgctaaaa atcatcaaag aaaacatcga aattacgaaa
    40321 gaaccagaga aatttaccac aataaaactt gatgcggaca aagcaatcga acggctagca
    40381 gcggagaaac aacaattcga ctacttgttt cttgatccgc catatgcaaa acaaaaaata
    40441 atcgatcaaa tccataaaat ggaagaactg ggactttttt ctaaaaccgc agttatcgta
    40501 tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg ctggattcca tcaaataaga
    40561 aaacagattt atggcataac ggccgtaact atctatagaa aagagggaaa tcaatgaaac
    40621 gtgcattatt cccaggaagt tttgatcctt ttacaaaagg tcacttagat acggtggaac
    40681 gtgcggctaa attattcgat gaggtagtaa ttggtgtttt tatcaatact tcaaagaaaa
    40741 gtttgtttcc cccagaagaa agaatgacat tgatcacaaa agctgtttct catcttccta
    40801 atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa gagattggtg
    40861 cagatgcgtt gatcagaggc atacgaagta tcaaagactt tgaatatgaa cgagagattg
    40921 ctcagatgaa tcatcatcta tatgatgaat tggaaactgt cttcttatta gcaaaaccag
    40981 aatatagtca cgtgagttcc agtattttaa aagaagtact gcactttggc ggagatgtga
    41041 gcagctactt accgccagtg atcaacgaag ctttagctgg aaagcgtgaa tcaaatgaaa
    41101 gctaaacaat ggttgaaaac agtactgatt atcatatttg gaatgttgat cattggattt
    41161 gtagtagttc ccttgccgta ttatgtagaa tcaccgggag ccacgatcga tctgaaagaa
    41221 ctaattacgg tcaatgataa ggaagataaa ggttctgggt ctttctctct tacctctgtt
    41281 ggtatcaggc aagctacagg tttcttagct ttaaaagcta aaatgacccc ttttgaagaa
    41341 ttgatcagtg aagaagaatt gactggtggg gcgacaaatg aagaatataa tcaaatgcaa
    41401 cagtattata tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa acttgcagac
    41461 aagccttata aaatggaatt caaaggcgta tatgtcatga gtgtagagaa aacatcgaat
    41521 ttctttggca agctttcagt tggagataca gtaacaaaag tagacgggaa aagctttcaa
    41581 agtacagaag catttatgga ttatgtaaaa agccaaaaag ttggacaaac aatcgaaatc
    41641 agttatttgc ataatggtga agaaaaaaca gcttctggtg atctgatcga gttaccaaca
    41701 gataaaaagg caggaatagg tatcacgctt actgaccata ccgagatcga atcagataca
    41761 gatgttcgta ttaattctgg ctctatcggt gggccatcag cgggattgat gttcacttta
    41821 gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat tgcaggaact
    41881 ggaacgatca acagtgaagg agaagttggc cgaatcggag gaatcgataa aaaagtggca
    41941 agtgcggata aagctggcgt agagattttc tttgcaccag atgatacgat ttcagacgat
    42001 gttaagaaag aatatcctga aataaaaacc aactacgaag aagcaaaagc tgccgctaaa
    42061 aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc gcttgactat
    42121 ctagagaaaa tgaaatgaaa ctaaataatt aaag
//
LOCUS       DAYYOA010000012        41165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000012 DAYYOA010000000
VERSION     DAYYOA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.012"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1233)
                     /locus_tag="ACHBKA_000595"
     CDS             complement(451..1233)
                     /locus_tag="ACHBKA_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444310.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="ACHBKA_000596"
     CDS             complement(1298..2134)
                     /locus_tag="ACHBKA_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9444311.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="ACHBKA_000597"
     CDS             complement(2152..3561)
                     /locus_tag="ACHBKA_000597"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9444312.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="ACHBKA_000598"
     CDS             complement(3575..5398)
                     /locus_tag="ACHBKA_000598"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9444313.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="ACHBKA_000599"
     CDS             complement(6241..6567)
                     /locus_tag="ACHBKA_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444314.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="ACHBKA_000600"
     CDS             complement(6613..6975)
                     /locus_tag="ACHBKA_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM9444315.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="ACHBKA_000601"
     CDS             complement(7012..7227)
                     /locus_tag="ACHBKA_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9444316.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="ACHBKA_000602"
     CDS             7354..7689
                     /locus_tag="ACHBKA_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9444317.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="ACHBKA_000603"
     CDS             complement(7765..9762)
                     /locus_tag="ACHBKA_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444318.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="ACHBKA_000604"
     CDS             complement(9860..10135)
                     /locus_tag="ACHBKA_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444319.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10367..11101
                     /locus_tag="ACHBKA_000605"
     CDS             10367..11101
                     /locus_tag="ACHBKA_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9444320.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11140..12282
                     /locus_tag="ACHBKA_000606"
     CDS             11140..12282
                     /locus_tag="ACHBKA_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9444321.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="ACHBKA_000607"
     CDS             complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="ACHBKA_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM9444322.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13773..14243)
                     /gene="sufU"
                     /locus_tag="ACHBKA_000608"
     CDS             complement(13773..14243)
                     /gene="sufU"
                     /locus_tag="ACHBKA_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM9444323.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14230..15465)
                     /locus_tag="ACHBKA_000609"
     CDS             complement(14230..15465)
                     /locus_tag="ACHBKA_000609"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM9444324.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="ACHBKA_000610"
     CDS             complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="ACHBKA_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM9444325.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="ACHBKA_000611"
     CDS             complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="ACHBKA_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM9444326.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17731..18579)
                     /locus_tag="ACHBKA_000612"
     CDS             complement(17731..18579)
                     /locus_tag="ACHBKA_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9444327.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18603..19289)
                     /locus_tag="ACHBKA_000613"
     CDS             complement(18603..19289)
                     /locus_tag="ACHBKA_000613"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9444328.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19282..20316)
                     /locus_tag="ACHBKA_000614"
     CDS             complement(19282..20316)
                     /locus_tag="ACHBKA_000614"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9444329.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20379..20635)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20711..21049)
                     /locus_tag="ACHBKA_000615"
     CDS             complement(20711..21049)
                     /locus_tag="ACHBKA_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM9444330.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21051..21407)
                     /locus_tag="ACHBKA_000616"
     CDS             complement(21051..21407)
                     /locus_tag="ACHBKA_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM9444331.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21413..22600)
                     /locus_tag="ACHBKA_000617"
     CDS             complement(21413..22600)
                     /locus_tag="ACHBKA_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9444332.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22791..23636)
                     /locus_tag="ACHBKA_000618"
     CDS             complement(22791..23636)
                     /locus_tag="ACHBKA_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9444333.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23870..24835
                     /locus_tag="ACHBKA_000619"
     CDS             23870..24835
                     /locus_tag="ACHBKA_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM9444334.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25212..26255
                     /locus_tag="ACHBKA_000620"
     CDS             25212..26255
                     /locus_tag="ACHBKA_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM9444335.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26317..26781)
                     /locus_tag="ACHBKA_000621"
     CDS             complement(26317..26781)
                     /locus_tag="ACHBKA_000621"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM9444336.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27027..27974
                     /locus_tag="ACHBKA_000622"
     CDS             27027..27974
                     /locus_tag="ACHBKA_000622"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9444337.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28185..30257)
                     /locus_tag="ACHBKA_000623"
     CDS             complement(28185..30257)
                     /locus_tag="ACHBKA_000623"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9444338.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30190..30567)
                     /locus_tag="ACHBKA_000624"
     CDS             complement(30190..30567)
                     /locus_tag="ACHBKA_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM9444339.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30696..31256)
                     /locus_tag="ACHBKA_000625"
     CDS             complement(30696..31256)
                     /locus_tag="ACHBKA_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9444340.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31406..32698
                     /locus_tag="ACHBKA_000626"
     CDS             31406..32698
                     /locus_tag="ACHBKA_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9444341.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32758..33876)
                     /locus_tag="ACHBKA_000627"
     CDS             complement(32758..33876)
                     /locus_tag="ACHBKA_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM9444342.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="ACHBKA_000628"
     CDS             complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="ACHBKA_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM9444343.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="ACHBKA_000629"
     CDS             complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="ACHBKA_000629"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM9444344.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37082..37681
                     /locus_tag="ACHBKA_000630"
     CDS             37082..37681
                     /locus_tag="ACHBKA_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9444345.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="ACHBKA_000631"
     CDS             complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="ACHBKA_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM9444346.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39376..39720)
                     /locus_tag="ACHBKA_000632"
     CDS             complement(39376..39720)
                     /locus_tag="ACHBKA_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM9444347.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39857..41002)
                     /locus_tag="ACHBKA_000633"
     CDS             complement(39857..41002)
                     /locus_tag="ACHBKA_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9444348.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11848 a         8652 c         7193 g        13472 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc
    10321 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    10381 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    10441 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    10501 caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    10561 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    10621 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    10681 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    10741 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    10801 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    10861 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    10921 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    10981 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    11041 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    11101 gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac
    11161 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    11221 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    11281 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    11341 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    11401 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    11461 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    11521 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    11581 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    11641 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    11701 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    11761 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    11821 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    11881 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    11941 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    12001 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    12061 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    12121 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    12181 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    12241 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    12301 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    12361 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    12421 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    12481 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    12541 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    12601 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    12661 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    12721 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    12781 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    12841 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    12901 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    12961 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    13021 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    13081 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    13141 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    13201 cgccttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    13261 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    13321 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    13381 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    13441 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    13501 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    13561 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    13621 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    13681 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    13741 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    13801 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    13861 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    13921 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    13981 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    14041 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    14101 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    14161 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    14221 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    14281 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    14341 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    14401 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    14461 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    14521 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    14581 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    14641 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    14701 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    14761 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    14821 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    14881 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    14941 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    15001 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    15061 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    15121 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    15181 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    15241 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    15301 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    15361 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    15421 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    15481 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    15541 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    15601 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    15661 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    15721 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    15781 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    15841 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    15901 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    15961 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    16021 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    16081 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    16141 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    16201 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    16261 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    16321 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    16381 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    16441 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    16501 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    16561 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    16621 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    16681 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    16741 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    16801 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    16861 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    16921 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    16981 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    17041 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    17101 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    17161 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    17221 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    17281 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    17341 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    17401 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    17461 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    17521 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    17581 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    17641 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    17701 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    17761 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    17821 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    17881 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    17941 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    18001 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    18061 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    18121 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    18181 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    18241 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    18301 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    18361 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    18421 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    18481 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    18541 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    18601 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    18661 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    18721 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    18781 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    18841 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    18901 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    18961 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    19021 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    19081 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    19141 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    19201 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    19261 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    19321 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    19381 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    19441 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    19501 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    19561 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    19621 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    19681 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    19741 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    19801 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    19861 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    19921 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    19981 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    20041 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    20101 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    20161 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    20221 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    20281 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga
    20341 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    20401 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    20461 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    20521 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    20581 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    20641 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    20701 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    20761 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    20821 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    20881 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    20941 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    21001 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    21061 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    21121 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    21181 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    21241 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    21301 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    21361 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    21421 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    21481 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    21541 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    21601 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    21661 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    21721 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    21781 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    21841 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    21901 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    21961 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    22021 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    22081 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    22141 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    22201 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    22261 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    22321 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    22381 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    22441 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    22501 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    22561 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    22621 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    22681 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    22741 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    22801 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    22861 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    22921 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    22981 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    23041 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    23101 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    23161 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    23221 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    23281 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    23341 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    23401 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    23461 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    23521 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    23581 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    23641 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    23701 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    23761 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    23821 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    23881 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    23941 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    24001 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    24061 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    24121 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    24181 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    24241 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    24301 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    24361 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    24421 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    24481 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    24541 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    24601 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    24661 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    24721 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    24781 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    24841 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    24901 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    24961 cgtttaccaa gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga
    25021 aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa
    25081 caccgtttat tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg
    25141 tcaaaatact ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag
    25201 gaaccgatca aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc
    25261 gcgaaatctt acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg
    25321 ctatttatct gccacagtta ggtggagcga ctattgctat tttattgggt attttattag
    25381 gaaatacttt ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac
    25441 tattagaatt ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa
    25501 ttggtgtaaa aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt
    25561 acttgattgg taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa
    25621 acgctgtttg tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg
    25681 aagaaaaagg acagatcatc acactagtca atctactggg tactgtgatg atgttgactc
    25741 tcccttttct tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg
    25801 gtggtacact ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg
    25861 ttgtcgaatt cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct
    25921 atctgtttga aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata
    25981 cgcagaaaaa acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt
    26041 tggtcaacag cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca
    26101 gctggtttga gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca
    26161 tgaaagaagg tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga
    26221 tggctttact atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga
    26281 ttctcgcatg gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa
    26341 caacaacaat tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag
    26401 ttttccattt tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg
    26461 atttgcctgc ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt
    26521 atacgttcgg atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa
    26581 aggcgtacct ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata
    26641 ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga
    26701 aattggctga aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc
    26761 gataggtgtg tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt
    26821 tttctctatc caaaactact tgccaactaa taaaaaatgt tcttaaaagt ttgataaacc
    26881 gaggaaacat ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta
    26941 aaatcaaatt ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata
    27001 aacatacgat tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg
    27061 aagaaaaaac tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt
    27121 tcgaatattt gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc
    27181 tagcctcagc ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag
    27241 cggctctgta cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg
    27301 atctgcaaga tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata
    27361 tcgattgtgt cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct
    27421 ttgcgaaaaa attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg
    27481 ctcgtgattt ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat
    27541 acaaccgatt ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt
    27601 atgaaaaccg cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact
    27661 actcgattga tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg
    27721 gcgctttgtc ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt
    27781 tgtttggtga tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg
    27841 gaatccaatt gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa
    27901 cgaaacagcg tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga
    27961 agcctcacca ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt
    28021 gacaaaagtc ttgtcacagt ccttttttcc gaataaatgg tgttccagaa gtagcttctt
    28081 cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
    28141 ttcttgaagc tgaccacttc tatcacaacc tctttcatat cttattactt cttcattgct
    28201 gcttttagtg ctgctgctaa tgggctttct tctggttctt ctgcttgtgc atattttttc
    28261 agcaagcgtc gttcttcatg tttggtcatt ttcttcttct gctctttttt atcaagcaat
    28321 ttttctgtcg ttccatcgta tttacaacgg aaataagcac cgtttttacc ttcgatgatt
    28381 tccattttcc ggtgacattg cgggcatcga tgattagaga cttttggatc ttttcttctg
    28441 cggtagttgc aatctgaatt ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg
    28501 cgcagattgg ccccacattc tgggcatttc ttttgggtaa tcgagaaatc ttgatattct
    28561 tgtttgcttg atttgatctc attcaccaag cgttttgttt ctttttcgat tccattcaag
    28621 aaggttcctg cttgtttctt tcctgcagca atagcttcaa ggtctttttc ccagctttct
    28681 gtcaaatctg gtgttactaa agagggattt accaatacta ataattgctt acctttagga
    28741 gatacttgca gtccttgagg ggtacgttcc atcagttcag atttgattag tttctcgata
    28801 atctctgcac gcgtagctgg ggttcccaga ctgtattttt ccatttgacc aagcaagctt
    28861 gcttctgaca atggtttagg cggtgcagat agttcttttt tgatggcgaa atcgggattg
    28921 attctcattc ctttttccca tttgactgtt tccgtctcag tttcagtttc gcctttccat
    28981 cctgcttgga cgacacgatt ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta
    29041 acttttgttt ggctgacttt atggggttga gcaaacagtc ctaaaaaacg agagacaatc
    29101 atttgataga tttttcgctc ttcattactt aatttttcat aacgtgctct gttttcagtt
    29161 ggcaaaagtg catggtgatc ggtcacttta ctgtcttgga atacttttgt ctgttggacg
    29221 atggctccgt ttttcaaata ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat
    29281 cgttctttca tcgtactttt catgtcattt gtcagatatt tgctgtctgt acgcggataa
    29341 gtaacgatct tatgggtctc atacaaactt tgtacaagac ttaatgtttt ctttgcagaa
    29401 tagccatagc gttgattggc ttctctttgg atctctgtta aatcataagg caatggcgca
    29461 gattccgttt ttaatttttc ttggacttct gtgacagttc ctttgcttgt actgatcaat
    29521 tgaacaagtt tttgtgcttc ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt
    29581 ttggcttgtt cttttccagc ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc
    29641 tcgattttct tttcttggtc tctcactaat gctagtgtcg gtgtctgcac acggcctgct
    29701 gataaattat ccgcgtactt cacagttaag gctctggtca cattcaatcc taccagccag
    29761 tccgcttttg cccgggcaag agcagattca tacaatgcat catattcttt cgcaggtctt
    29821 agttgcttga atccagtttt gattgcttta tctgtttgag aagagatcca taaccgcttc
    29881 actggtttat tgaaatgcac atactctaag atccagcgag caacaagttc cccttctctt
    29941 ccggcatctg tcgcaatgac tgcttctgag acatcttttc gctgagccag ctgcttgatt
    30001 gcttttaatt gatgtcctgt tttaggaagt ggtttgattc ctagtttttt agggatcatt
    30061 ggcaacgtgt ccatttgcca gctctgccat tccttattca gatcttccgg cattttcaat
    30121 cctaataaat gaccaagggc ccaagtaacg atcaccttgg gtccttcata ataatttttg
    30181 tgcttttgat tagcaccaag caccttgctt aggtctttcg caacgcttgg tttttccgct
    30241 agaattagtt gtttcataaa attcctttct gctttctact gtgatgggct gatgattttt
    30301 caacaacgca agtcccttgt attgctgtaa atcatcgtca cactcttcac accagcgctc
    30361 atctccgtca ttccaaaagg cgctgagaaa gttacttttt gcattgatat attcataatt
    30421 ttgatggatt tcgttccatt gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa
    30481 tgtttcttct tcttcaatat cttcttccca gtcttcaaaa aaccaccaag gctcattgtc
    30541 cccgaacatg gtaattactt gatacatact cgtgctcctt ctttcagctt ttctagactc
    30601 atcttagtca cttttcaaga aagttgcaag aattttctct tataaaaggg tcatttaatg
    30661 aacatcttaa aattgttaca aatgaatctg attgtttaag gcaaagtagc gataaagtta
    30721 ttgatcatcc aaaaatagct gtcttctgga tcgattgttg gttcttttgt aaagtatcct
    30781 aaaaaacgta aggaaagata accgtggatc atactacgaa gttccctgct tcgatgaact
    30841 ttttcttctt tcgttaattg aaaagcctca aatatctgca aaatgatctg attcgtttca
    30901 tggattccat ctaataaaac agcttcattg gtatgcggta cactgatcag taactcatac
    30961 actgcttgat ttgaaaaagc aaaggatcga taagtttctg catagatctg cagtgcttcc
    31021 tgaccacttt ttcccacaag agcccgtcgc agctggtcat taagttcctg cagaagtttc
    31081 gctgttagcg ctgttttgac atcacgaata tttttgaaat ggttatacag cgagggagcc
    31141 ttgatatcca agcgtttggc taaatgctga aaggttattt gctgaacatc catctcttct
    31201 gctagttctc gaaagcagtc gatgattttc tcttgagaca gatttctttt tttcatttcc
    31261 atgtttcctc tcataaaata aatccttcac tctttcagtt taccacgaaa aagaaagaga
    31321 aaaaagagac tttcattgat aaactacata ttgtagttta taataaagag ctgtagatga
    31381 aaattttcag aaagaaggag tctaattggc tgcaagaata aaggaaaaat tatttaggaa
    31441 aagagaaact tgggaacaaa acttgtttgt cttatggttt ggaacattta tggctggtat
    31501 cggatttagt ttagtcatgc cttttatgtc actttacata gatacgttag gtaattatag
    31561 tacgtcacaa ttgaactttt ggagcggatt gactttttca tctactttct tggtgactac
    31621 gcttatctct ccttggtggg gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt
    31681 gcgtgcttca ttaggaatgg caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta
    31741 tcaattagtc gcattgcgtc tgcttcaagg gattttttct ggttatatca gtaatgccac
    31801 tgcccttgtc gcaactggga cgccaaaaga aaaaagtggt caagttcttg gaaccttagc
    31861 aactggttct gttacaggaa cattacttgg accgcttctt ggcgggatct ctgcttcagc
    31921 gtttggctat cgccctacct ttttcattac agggatcatc ttatttttcg tcttcttgtt
    31981 gagtctgttt ttcgtccatg aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc
    32041 caaacagatt ttcaaagact tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac
    32101 gatgatcatc caagcatcca acaattccat tagtccaatc atcagtctgt acattcgcca
    32161 attgctgcat ggacatggga atgttacgtt gatcagcgga atcattgctt cgatacctgg
    32221 gattgccact ttgatagccg ctccaagatt tggtcgctta ggtgacaaga ttgggagtga
    32281 aagaatactt gcgattggtt tgggttttgc gattctggtt tatatcccaa tggcattcgt
    32341 cacgaatgtt tggcaattag ctgccttacg ttttttgatt ggtatctctg atgcttgtct
    32401 gctccctgct gtccaagcat tgatcacaaa atattcgcct catcaagctg ctggacggat
    32461 cttcagttat aaccaatctt tccaagcaac tggtaacgta gtagggccta tgatcggctc
    32521 cagtgtttcc agtgcatttg gttatcgtgg cgtctttctt tcgacttctc ttcttgtttt
    32581 ggtcaacttc ttgcttgtac gacacaatac acaagagatc catcagcaag aaactgcaga
    32641 agaaaaagct tcaggagtct ccatagcttc agggcactct ccttctcata gacaataagc
    32701 gtaaatgaaa aacactgctt gaacagttgt cacagactgt ccttgcagtg tttttgttta
    32761 ggcctgtttt ttcaagaact cagataagag gaataatccc ttttccaagg tttctttttt
    32821 acaacagtaa cctagtcggg catgcttcgg taaatcaaag gcacttcctg gtactaatag
    32881 tactcctgtt tcttctaata aattgataca aaatgtctgg tcatcttctt cgatggctaa
    32941 tttaatgaaa gaagtagaga cgtaattggg agcgacaagt tcaactttcg gttcattggc
    33001 tacccattgt tttaaaatca caaggttctc tgtaatgatc tttttgtttc gcgccaatat
    33061 tttttcttta ttcttcaatg catgtacagc aagatcatct gacaggactc cgccgcaaat
    33121 catcgtgtag tctcgatact ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca
    33181 gccaatacga atacctggaa tggagtaggt tttggatagg gaattggttg ctataccttt
    33241 ttcatagaca tctacgatcg acaagaattc acctttatcc gttaaaggcg catatacttc
    33301 atctatcaat acatatgcat ctactgtacg agcaatttct gcgatttttt ctaaagtttt
    33361 ccgatctagc aatgtgcctg taggattgtt tgcactattc aaacagatga tttttgtttc
    33421 aggtttgact aaggcttcta gctgttctaa atcaaactgc caatcttctt cttccttcaa
    33481 atgaacaaag tcaacagcgg cacctaaaga tttggggatg tcatataatt gctgataagt
    33541 aggaagcatc gaaacaacat ggtctcctgc ttccactaac gcgtatagag ctaaaagatt
    33601 agcaccagtc gctccattcg tttgtaagat gttttccgga tcgacggttt gataaagaga
    33661 agcgacttct tgtttgaaaa catctgatcc ttcgatccag ccatagtcca atggttcgtt
    33721 tttgcgtttt tcgaaaaatt tacttacgct agtcccatct aagccaacga gttcgtctaa
    33781 tgtcaatgct tcgatcgagc tttgtgcaat atcatatacc gctttctttt cccatttatt
    33841 tagccattct tctacaccga aatttgcgat tttcatgttt ctttccccct tatatcttga
    33901 tccagcctac agcaagcaac ccgattgaaa gcagtgcggc cacacaaatc agcccaatca
    33961 ttactttttc ttttcccgta atttgatgtt tggtttcacg acaagcgatc cagtagaagc
    34021 aaaaaccagg aagataggca atactacata aaaggacata agaaaatcct gctaaaatga
    34081 tgcccatcaa ttcaaaaact acagcaatcc caccgatcac tacttgtttt ttgattcctt
    34141 cttttaaaga aaactggact tgatagatcc cgacaagtag ataacaaatc aaaattgccg
    34201 ctgtacataa agaataggca aattgatagg cataatcagt aactaagaaa gtcaataaga
    34261 atagattagt caagattgca gtaacaacta acgaataagt tggggcattt tttttattcg
    34321 tttttcccca acctttcggc aacagaccgt ctcgagccat caacatcgtt gtttctgctg
    34381 ggagcatcgt ccaagacaac caagaaccaa tgatcgaaat aatcaagccg atattgatca
    34441 gcgctgctcc ccaatttccc acgattcctt ttaaaatatt tgccatagca ggttgtgaaa
    34501 tagttgctaa ctcttcttga gataagacgc cgtatggcaa gatcgaagca aggatataga
    34561 taaacaataa acaaagcaga ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg
    34621 cgcggttggc taaaacactt gcgccttcga tccctacaaa gacccacatc ataaccatca
    34681 aacaaccttt aatctgcaac cagatcgttc cttgctgccc tgttccattc actagattat
    34741 cagatacgtt accccaaaaa tcagcagtga atatattcac tttaaatgcg ataaatacaa
    34801 atattaaaaa taggaataat ggcactaatt tacagatcgt cacgattgta ttgatgacgc
    34861 tcgctccttc aactccattg tttactaata aagtcagacc ccaaataaaa atacttgcaa
    34921 ataaaataga aggaacattt tgtccacctt tgaatatcgg gaaaaattct cccatggcgc
    34981 tcatcagcat cgttgcaaaa gcaacatttc ccagccacgc agacaaccag taaccccaac
    35041 cactgatgaa gccacctaat ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat
    35101 ctagatctgg acgcttttct cctaaatgat tcagcgataa taccaaagct aaaataccaa
    35161 tcccaacgat tatccatgaa aggatcgctg gccctggagc tgcagaacta gccagatcac
    35221 ttgtgatccc aaacacaccg cttccgattg acgaactgac aaccagagca accagtcccc
    35281 atttgctaat acccgtcgat ttcttttcca tttccatcac cctcacttat aaaattgaac
    35341 aaatcacttg caaaagattc ccttctattt tgcaactgct cttgcttaac aaaaagcaac
    35401 tttattcata ttattggata ttatgaactt tttacacaag tatttcaagt ataaaatgaa
    35461 taaaaattaa ttttattgca aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt
    35521 tgttagccat agctgttttg attcatttaa tatcgcttaa acgtattgta aaacttttgt
    35581 ttcttgatat gcgtggtcga tcaaaccacc acccagacat tccattccat cataaaatac
    35641 aactgcttgt ccaggagtga ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt
    35701 atcatccaat aagcgaactg tcacaggaac gtcttgctga cgataacgga atttcgctgt
    35761 acacttgaat tcttttggca ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa
    35821 gcttgtggca tacaatgcag gatgatggaa cccttgcccc acatataatg tgtttgtcgc
    35881 tagatctttt cctacaacaa accagggttc ttgcgaatct cctccgccac cgatccctag
    35941 tccttgacgt tgaccaatcg tataatacat caagccagca tgttgtcctt tgacttcacc
    36001 atcaagagtg accatatttc ctttttttgc tggcagatag ttgctcaaaa attgtttgaa
    36061 gttcttttca ccaataaagc agattcccgt tgaatctttt ttcttagcag tcgcaaggcc
    36121 tgcgcgttca gcaattgccc gtacttctga tttttccatg ccgcctagag ggaacatcgt
    36181 ttttgaaagc tgttcttgtg ataattggct aaggaagtaa gtttgatctt tattattgtc
    36241 tactccacga agcatgtgga ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc
    36301 agtagctaca taatcggcgc caagatccat tgcataatcc aagaatgctt tgaatttgat
    36361 ttctttgttg cacatcacgt ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa
    36421 atattcaaat acacgatccc aatattcttt ttcaaaattc acagagtaat aaggaatccc
    36481 gatttgagca gctactttcg ctacatcctt ataatcttcc gtggccgtgc atacaccgtt
    36541 ttcgtccgtg tcatcccaat ttttcatgaa tattccgatc acgtcgtatc cttgttcctt
    36601 taacaaaagt gctgttacag atgagtcgac accgccgctc atccctacga caacgcgtgt
    36661 tttgctgttg tctgtcatga tatcaccatc atttcaatta gattctgaaa catctacgag
    36721 ttgaaggtcg caacttttca gtattttaca ttatacgtca tatttctgaa aatttcatcc
    36781 cttaattatc aagtcgtaaa aatactgttc tactggacct attcagtaca caaacatgat
    36841 agtcaaccaa aaaatcactc atcttttgag cattcgatat aaaaaattgg catactcata
    36901 aaaagggcca gcctttcggc aataaggaat aattttataa attgtgaaaa actatttttc
    36961 aaaattgctt cttatcgctt aaagctgcac gtctcattta caaccttttc tttttagatg
    37021 ttatgagttg ctcagataag tgattttgtt tactctaaga aaaaagggag gcaaaagata
    37081 catgaattac tttatcagtg atatgcactt tttccatgaa cgcttattag gaaaaaacga
    37141 ctttgctccg cggctctttg caaatgtgga tgaaatgaac caacagttga tcactagctg
    37201 gaatgcagta ataaaagaaa ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc
    37261 gcaatatgaa aaagcaaatg cagaaatttt ggaactggtc actcaactgc aaggaacaat
    37321 ccacttgatc aaagggaacc atgacagtcg cgcttttttc cgttatttag aaaaaaatga
    37381 tcctaggctt actgacggaa gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa
    37441 attcgaccat caacaatatt acttgactca ttatccgatg ttgctcgggc ctaacgacac
    37501 tatccgcaat cttcatggac atatccatca ttacagcgta cctatcgaga acgatgtcaa
    37561 tgtaggcgta gacgcaccgg aaagagaact cttaaaggaa aaactaccat ttggcactcc
    37621 gttatctgag caacaaatca aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg
    37681 aaaaaactgc cctatatcaa gtattaaaca aataaagata attactcata tttttcattt
    37741 gagtaggctt attgccacag gaacattagg atcagaaatg gagttgtaac aagacttctg
    37801 tcacaactcc attccatact attcgtcttt ttgctcatgg tgttctttta ccagtttttt
    37861 ctgtaattct ttatcataat ttcgggcagc tgacgttaaa aataaaacga ttccgcataa
    37921 taaaatcccg attccggaaa acaagaacat tttttctacc cctaaacgat cagctaatgg
    37981 tcctgcaaat aataatccaa ttggacctgt caggttcatc aaagagttga agttccctaa
    38041 gacacgacct aaaacattcg attcataact ctgctggatc attgccatca gcagtgtatt
    38101 gaaatatggc gttgcacaac ctgctcctgc attcaagata agaaagtaca agaacccttg
    38161 actatcgttt ggaaccaatc cgcttgcacc gatcgtcagg cccataatga tgtaggctat
    38221 aataataggt ttcattcggt ctttccatgt tcctataaaa ctgatcagtg caccgcctaa
    38281 gagcattccg actgcgtaga ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa
    38341 gtaatctagc gtcatcaatg gatacagact gattgctggc atgaacaaca gcatcacaaa
    38401 tgcaccattg atcgttatgt accataaccc tttattatcc atcaattgct gcagcccaaa
    38461 ttttgcatct ttaaaaatag taagcggaac ttccaatatt tcaggagaaa cttttgggat
    38521 cttcacaaaa atcaataagc cgacaccaaa aactgccccc aatacatcta ataaaataag
    38581 gtaattgaca ggaacgactg aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt
    38641 cgctgattgg accattccta actgtccatt cgtccttgtt atgtgagaag atggaacgat
    38701 tgtcggcata atcgactgga tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa
    38761 caaagacaca aaaactagcc aaatcgggaa ggattctgaa atcgttccga ctactgctaa
    38821 aataagcgcg aaaatcgcta caataatatc cgtgacaatc aaaagagctt ttttatccca
    38881 gcgatccact agcggtccta caaatgggct tagtaaaacc atgggaatca ttcctagtaa
    38941 agtagcaaaa ctcaaaattg tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc
    39001 atattggacg accatactag tgattcctga caaaaactgt cccgataaaa agaacaaata
    39061 atttctcttc caattcgtta aaacgaccga ctgttcattt tccataaaca tccctctttt
    39121 ctttttcatt ttgtatagcg tttgcacaac aatcattata cgggattctt ttttcaaaaa
    39181 ataccttttt ccctaaataa caatcgtttc aatttaaatt tctttactta gaaacaataa
    39241 atcagcaaaa tattaaaaat aacaacatgt gttaaattta aaaataaaaa agacaactaa
    39301 tttgcgtcgt ccttacaaag ttagtcatct aacaataggc cgtttcaaaa ctagttgtct
    39361 ttttatatcc acagatcatt cgactttttc gaataaccca cggctaatca tcccatccat
    39421 cagaaaataa aatttatctt ggctcatttt ctgttttgga tctttgaaaa tattgactgc
    39481 tttcaatcca tttgctgtag tgatcgacat tttcaatgtt tgaatatctt ttgatacagt
    39541 gatttttaat ttgaatcctt ctttgctttg cggcgtggca tctaatggtc ggatcaattg
    39601 ataattgcct ccgtttgttt ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc
    39661 aggatgcaaa cgatagctaa ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat
    39721 gattttcacc tcgataaaat tagtttctct ttattataaa agtttatcct gatttttact
    39781 aggtttttca gttaagttcc ctaaaaaatg aaggcgttct ctcagacatc tgttcatata
    39841 agaatgactt cactagttat tttttgacag cacgaactaa ttgatcaagg aaatactgga
    39901 tgtcgtcttc ggtatttccc aaaccaaagc tgatacggat ggattctttt agtatcggtg
    39961 tatcttctcc atacattgct tccaatacgt gtgaaggctc gatatttcct gctgtacaag
    40021 ctgaaccagt agaaatagcg accccattaa gatcaagctt catcagtagc aaatcatttg
    40081 cttcgccaat taagcgaaga ttcaacacat gagggagttt attcgttaaa tcaccattta
    40141 cttgataagg gatttcgttt gcttctaatg tatcaagaat caattgctgg aatcctttat
    40201 atttgttgtt acgtgcttca cgttcttcag gtgacagcag ttcaactgct tttgccatac
    40261 cccagatccc tgctaaattt tctgtacctg cacgccgctt ttcttcctgc tccccgccta
    40321 aaagcaatgc agggatattt actccgtctc gtttatataa gaaaccgacg ccttttggcc
    40381 cattgatttt atgagcagag atacttaata aatcgattcc taattcttct ggatggatcg
    40441 tttggtttcc gtacgcttgt accgcatctg tatggaaata agctggatga tccgctaaaa
    40501 gttcaccgat ttctttgatt ggcatcaaat ttccgatttc attgttcccg tacatcacag
    40561 agaccaagat cgtatcttct cgtaacgctt ttttgacctc ttcgatcgac agattccctt
    40621 tttcatctac aggcagatat gtaacgtcaa acccctttgt ctctaaatat ttcatggtat
    40681 taagaacagc aggatgttcg atagctgtag tgattagatg tctgccttct ttttgacgcg
    40741 agaacgctgt ttctaagata gctgtattat ccccttctgt tcccccactg ttaaagatga
    40801 tctcgtgagg ttttgcttgc agactgtctg caatgatttg tcttgctgtt tccaattttt
    40861 catgcgcgtg gcgaccaaat ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga
    40921 ttgccgtcat cgcttcaatt acttgtggat gcatgggggt agttgccgca tgatccagat
    40981 aaatagtttt cacttcattc atcccttcca aattattgct aggcacattc tagcataaaa
    41041 tagcgaacaa accaaagaag agctcatttt tgtctagagg acgtttcatg ggcatagaaa
    41101 aaaggagctg tgacaaaagt cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac
    41161 ttctt
//
LOCUS       DAYYOA010000013        39869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000013 DAYYOA010000000
VERSION     DAYYOA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.013"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..946
                     /locus_tag="ACHBKA_000634"
     CDS             77..946
                     /locus_tag="ACHBKA_000634"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM9444349.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1111..2382
                     /locus_tag="ACHBKA_000635"
     CDS             1111..2382
                     /locus_tag="ACHBKA_000635"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9444350.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2379..3674
                     /gene="rho"
                     /locus_tag="ACHBKA_000636"
     CDS             2379..3674
                     /gene="rho"
                     /locus_tag="ACHBKA_000636"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM9444351.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3773..4036
                     /locus_tag="ACHBKA_000637"
     CDS             3773..4036
                     /locus_tag="ACHBKA_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM9444352.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4481..4930
                     /locus_tag="ACHBKA_000638"
     CDS             4481..4930
                     /locus_tag="ACHBKA_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9444353.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4987..6405
                     /locus_tag="ACHBKA_000639"
     CDS             4987..6405
                     /locus_tag="ACHBKA_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9444354.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6405..7265
                     /locus_tag="ACHBKA_000640"
     CDS             6405..7265
                     /locus_tag="ACHBKA_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9444355.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7439..8212
                     /locus_tag="ACHBKA_000641"
     CDS             7439..8212
                     /locus_tag="ACHBKA_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9444356.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8359..8724
                     /locus_tag="ACHBKA_000642"
     CDS             8359..8724
                     /locus_tag="ACHBKA_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM9444357.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8872..10080)
                     /locus_tag="ACHBKA_000643"
     CDS             complement(8872..10080)
                     /locus_tag="ACHBKA_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9444358.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10099..10377)
                     /locus_tag="ACHBKA_000644"
     CDS             complement(10099..10377)
                     /locus_tag="ACHBKA_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444359.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10570..11226
                     /locus_tag="ACHBKA_000645"
     CDS             10570..11226
                     /locus_tag="ACHBKA_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9444360.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11668..12465)
                     /locus_tag="ACHBKA_000646"
     CDS             complement(11668..12465)
                     /locus_tag="ACHBKA_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM9444361.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12462..13646)
                     /locus_tag="ACHBKA_000647"
     CDS             complement(12462..13646)
                     /locus_tag="ACHBKA_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM9444362.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13671..14918)
                     /locus_tag="ACHBKA_000648"
     CDS             complement(13671..14918)
                     /locus_tag="ACHBKA_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9444363.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15133..16878
                     /gene="dnaX"
                     /locus_tag="ACHBKA_000649"
     CDS             15133..16878
                     /gene="dnaX"
                     /locus_tag="ACHBKA_000649"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM9444364.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16898..17212
                     /locus_tag="ACHBKA_000650"
     CDS             16898..17212
                     /locus_tag="ACHBKA_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM9444365.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17237..17833
                     /gene="recR"
                     /locus_tag="ACHBKA_000651"
     CDS             17237..17833
                     /gene="recR"
                     /locus_tag="ACHBKA_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM9444366.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17897..18541
                     /gene="tmk"
                     /locus_tag="ACHBKA_000652"
     CDS             17897..18541
                     /gene="tmk"
                     /locus_tag="ACHBKA_000652"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM9444367.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18556..18885
                     /locus_tag="ACHBKA_000653"
     CDS             18556..18885
                     /locus_tag="ACHBKA_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM9444368.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18899..19837
                     /gene="holB"
                     /locus_tag="ACHBKA_000654"
     CDS             18899..19837
                     /gene="holB"
                     /locus_tag="ACHBKA_000654"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM9444369.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19873..20697
                     /locus_tag="ACHBKA_000655"
     CDS             19873..20697
                     /locus_tag="ACHBKA_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM9444370.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20690..21037
                     /locus_tag="ACHBKA_000656"
     CDS             20690..21037
                     /locus_tag="ACHBKA_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM9444371.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21106..21978
                     /gene="rsmI"
                     /locus_tag="ACHBKA_000657"
     CDS             21106..21978
                     /gene="rsmI"
                     /locus_tag="ACHBKA_000657"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM9444372.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22087..23208
                     /gene="dinB"
                     /locus_tag="ACHBKA_000658"
     CDS             22087..23208
                     /gene="dinB"
                     /locus_tag="ACHBKA_000658"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM9444373.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23262..23864)
                     /gene="nrdG"
                     /locus_tag="ACHBKA_000659"
     CDS             complement(23262..23864)
                     /gene="nrdG"
                     /locus_tag="ACHBKA_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM9444374.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24179..26368)
                     /gene="nrdD"
                     /locus_tag="ACHBKA_000660"
     CDS             complement(24179..26368)
                     /gene="nrdD"
                     /locus_tag="ACHBKA_000660"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM9444375.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26694..27461
                     /locus_tag="ACHBKA_000661"
     CDS             26694..27461
                     /locus_tag="ACHBKA_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444376.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27451..29493
                     /locus_tag="ACHBKA_000662"
     CDS             27451..29493
                     /locus_tag="ACHBKA_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444377.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            29774..29923
                     /locus_tag="ACHBKA_000663"
     CDS             29774..29923
                     /locus_tag="ACHBKA_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM9444378.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            29934..31448
                     /gene="dltA"
                     /locus_tag="ACHBKA_000664"
     CDS             29934..31448
                     /gene="dltA"
                     /locus_tag="ACHBKA_000664"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM9444379.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            31445..32650
                     /gene="dltB"
                     /locus_tag="ACHBKA_000665"
     CDS             31445..32650
                     /gene="dltB"
                     /locus_tag="ACHBKA_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM9444380.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            32695..32928
                     /gene="dltC"
                     /locus_tag="ACHBKA_000666"
     CDS             32695..32928
                     /gene="dltC"
                     /locus_tag="ACHBKA_000666"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM9444381.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            32931..34199
                     /gene="dltD"
                     /locus_tag="ACHBKA_000667"
     CDS             32931..34199
                     /gene="dltD"
                     /locus_tag="ACHBKA_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM9444382.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            34340..35422
                     /locus_tag="ACHBKA_000668"
     CDS             34340..35422
                     /locus_tag="ACHBKA_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM9444383.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            35422..36225
                     /locus_tag="ACHBKA_000669"
     CDS             35422..36225
                     /locus_tag="ACHBKA_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9444384.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            36295..37035
                     /locus_tag="ACHBKA_000670"
     CDS             36295..37035
                     /locus_tag="ACHBKA_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9444385.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            37046..38050
                     /locus_tag="ACHBKA_000671"
     CDS             37046..38050
                     /locus_tag="ACHBKA_000671"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM9444386.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(38095..39867)
                     /locus_tag="ACHBKA_000672"
     CDS             complement(38095..39867)
                     /locus_tag="ACHBKA_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444387.1"
                     /translation="MLSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFK
                     FDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQ
                     EESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSV
                     PQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGIL
                     FEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTP
                     AELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTA
                     LGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQL
                     RQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVT
                     NLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLY
                     RKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGT
                     LTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        12788 a         7017 c         8277 g        11787 t
ORIGIN      
        1 gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt ttacaaaact
       61 taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag ctgctcgtaa
      121 aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc aagcaatcct
      181 tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg gtgcagctaa
      241 atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg aagcaatgaa
      301 catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg ctttggaatg
      361 tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat ttgaagaaaa
      421 cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct cagttgaatg
      481 tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg aattagctga
      541 tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt gcggtatcgg
      601 taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc acttacaagc
      661 aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat caggtattcc
      721 tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg ttaacactga
      781 attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta aagataaaga
      841 aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa tcgttgaaga
      901 tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat ccacataaca
      961 gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct tttttactta
     1021 aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag catatccatg
     1081 aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa aggaaaccga
     1141 cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc attgattcca
     1201 gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat acaagatgtt
     1261 cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc caacaacata
     1321 ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa aatcaatagt
     1381 ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga agcggtagta
     1441 ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt caaaggattt
     1501 gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg gacagaaaat
     1561 aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc aacgatcaat
     1621 gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc tgcacgagaa
     1681 ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt tcgaggagcg
     1741 ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg gcatttcatg
     1801 attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt aggaaatggt
     1861 atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa attgcaggaa
     1921 attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc gaaagaatta
     1981 aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct gcagcagcca
     2041 cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac gatctatgcg
     2101 aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac cgtggaagga
     2161 aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt ggcttctgat
     2221 cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac aacgacgatt
     2281 tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact aacagcctta
     2341 ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat ttgacaatgg
     2401 cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa tttaaaattc
     2461 cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt gcacaagcag
     2521 agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa gatggttacg
     2581 gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt tctgcctcac
     2641 aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc cgtccaccta
     2701 aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga aaagatccgg
     2761 aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg caaagacagc
     2821 ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc ttttctccaa
     2881 tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa acttctgtat
     2941 taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta atcgttctac
     3001 tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa ggcgacgtgg
     3061 tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa ttagtattgg
     3121 aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg gatagtatta
     3181 ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca ttgagtggcg
     3241 ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca agaaacatcg
     3301 aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga agccgtatgg
     3361 atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa ctttcacgtg
     3421 aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt acccgaaaag
     3481 aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga aaaaatatga
     3541 ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa acaaaaaaca
     3601 atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat ccgaccagaa
     3661 atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg ttgtaaatcg
     3721 acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa tcatgaaaga
     3781 aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg gtttcaaatt
     3841 cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg gtaatacata
     3901 cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg gacgtcaaaa
     3961 attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc tcaaagacgc
     4021 aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta tctgataggg
     4081 atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct gaaaaagtcg
     4141 taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta tccacttgat
     4201 aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa accatttacc
     4261 taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta tagacttgcg
     4321 attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga tgggtctttt
     4381 tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg acctcagttc
     4441 caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg atcaattgat
     4501 cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat tatttgagcg
     4561 aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag tcgttaaggc
     4621 actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg ggattcccca
     4681 tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag ccaaattaac
     4741 ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg tagttcctga
     4801 aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat tgatgaatgc
     4861 agattttaga aagggaataa aagaagccac tacgccagca gatatcatca gacagattag
     4921 tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa aaggaggaaa
     4981 ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca tacttatatg
     5041 tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa attcgaaaca
     5101 caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc agatgcaatc
     5161 attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa agtaaagcgt
     5221 gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc attgagagat
     5281 attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa aaagccgaca
     5341 atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat cgcaggcgga
     5401 atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa ctcagcagac
     5461 tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt tgccatgatg
     5521 gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg ttttgcccca
     5581 ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg cggaatgatt
     5641 ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag cgtaaaaaaa
     5701 gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt aattattggg
     5761 cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc tttgacaaat
     5821 tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat cgggatcatg
     5881 atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc gatcggtttg
     5941 atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat gacacctccc
     6001 ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga agaacggact
     6061 tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc tataccgttt
     6121 gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc agtcgctgca
     6181 gggatttcca tggcagtagg agttacttca atggcacctc atggagggat ttgggtgatg
     6241 ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc atcaggtaca
     6301 gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa aaaacaagac
     6361 aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta tatacaatga
     6421 aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc aatatttgca
     6481 gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca ccagtaatct
     6541 tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg gcagtaagag
     6601 cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc ggtacgatag
     6661 aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat gcgtctttga
     6721 aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct catgcggtaa
     6781 atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct gaaggaggtg
     6841 ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa gaaacaggaa
     6901 tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa aacagaactc
     6961 caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt ccatttgttt
     7021 tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa tacggagttg
     7081 ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg agagaaatat
     7141 tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct aataaaaagg
     7201 tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag gctagtttat
     7261 actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat accctcttaa
     7321 ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc tcgaagccaa
     7381 tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg gtgattttat
     7441 gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa acaaccaatt
     7501 tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga ttaaacgtga
     7561 ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg ccatgcttat
     7621 tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag aagaagaaac
     7681 agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata tggtcttatt
     7741 cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat ttgatggttt
     7801 gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata caacggcaaa
     7861 agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg gttcaaaagc
     7921 ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc gcgggctaga
     7981 tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa aagcttttag
     8041 aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac ataaattcat
     8101 gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag aactgacaaa
     8161 agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat aaattattac
     8221 gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc tcgcttctct
     8281 cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt ttttatccag
     8341 gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc aatcatgatc
     8401 tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat ggattcaacg
     8461 attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt gatcttacgt
     8521 aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt tatcttgtgg
     8581 ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt gattcctgct
     8641 ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca aggcatcgta
     8701 ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga taaatgaaag
     8761 ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta ctcaaaaaac
     8821 cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag atcaaatttc
     8881 atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc ggccattaac
     8941 aagatagagt gctgtacata tcaatggaat gactccggca atcacataca atcctctaaa
     9001 tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc ctaggtctaa
     9061 accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga tttttaaaca
     9121 aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg ctccagagat
     9181 aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg ttaagaataa
     9241 atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc cagtcaatgg
     9301 acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag cagcaactaa
     9361 gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag aaagacccat
     9421 caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat tgatattcca
     9481 tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag gaaagacaag
     9541 gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga aacgaaaatc
     9601 agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta aactagtact
     9661 taatccataa taattgatgc cttccccttt tttagatgca gggatatatg cagtaacgat
     9721 agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc gaactaaata
     9781 caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat agaagatcgc
     9841 gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca tgaataagcg
     9901 tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc tcaagctggc
     9961 gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat aaatcaggta
    10021 gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt tcttttccat
    10081 gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt gaacttctac
    10141 atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat ctcttgtaga
    10201 cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca ctgctttgat
    10261 caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt tcatatcaaa
    10321 tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt aattcatgtt
    10381 ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa aaaaggaaaa
    10441 aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta gtaaaatacc
    10501 tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag tttggatggg
    10561 ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga ttttccgatt
    10621 atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc ccatacgtat
    10681 atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag agaatttaac
    10741 cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata ttcacaattc
    10801 atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta tcgaatcaat
    10861 cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta tgtaaaagag
    10921 tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct attaaacgga
    10981 aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg tgtgcggaca
    11041 gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa attttgtggc
    11101 atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga tggtattatc
    11161 accgttaaag accatcatat cgttattttg aatatggact tgttagaaga caatattatc
    11221 ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac
    11281 ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg
    11341 aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggaacaatt
    11401 tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg agttggtagg
    11461 agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa taagaagagc
    11521 caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt agatgtaagc
    11581 ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt cacagccctt
    11641 tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc caaaagtccc
    11701 gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg ggcttttggg
    11761 gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag ttaatttgat
    11821 actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat aacgcgaaac
    11881 agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa tgatgatttc
    11941 tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta cacgataatg
    12001 tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag cttttgcagc
    12061 attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg aatcgattct
    12121 gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt cacgctgtcc
    12181 catatctaat agcaatactg agccattttg atcatttccg actgcgtctc ttaagtaatt
    12241 atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca agactcgtcc
    12301 tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt gatctgttgt
    12361 gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca ccggccagcc
    12421 aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa gtaacctccg
    12481 atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc caattttttc
    12541 tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc gtagtagtag
    12601 acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag taatacgtaa
    12661 ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt tgtatatcca
    12721 tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg aatctgtgat
    12781 ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa atactggctt
    12841 ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat cttttgttgc
    12901 gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc tgctcttgaa
    12961 acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat gcggataact
    13021 gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata agggaaatac
    13081 acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac aaaccatttt
    13141 ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc aaaaggaagt
    13201 actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg attatggcag
    13261 tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc acagttggga
    13321 caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt atctgaaaaa
    13381 ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag ctgttcttgg
    13441 gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc gccagccata
    13501 cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata gatattcagg
    13561 cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc gccgcaatta
    13621 ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa ttaattaacg
    13681 gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc gttggaaaga
    13741 tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac gttttctaca
    13801 ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc gcaaactgga
    13861 catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc tgcttgttgt
    13921 ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt tgcattcatt
    13981 cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc ggctgcttcc
    14041 ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc acctagcatc
    14101 gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga aatgctggct
    14161 acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc caatgcagta
    14221 tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg ttgaccatct
    14281 gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa atactgctca
    14341 ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt ataaaataaa
    14401 atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa ttctgcgtta
    14461 tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc ctttatttct
    14521 tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa tttgaatcgg
    14581 tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg agctgcgtca
    14641 tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc accatcgacc
    14701 aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa aacatcttct
    14761 gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc ttttgtcatg
    14821 accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc tgccagaccg
    14881 ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc ctctgattca
    14941 gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga aacttctcgg
    15001 actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa aaagccgaga
    15061 aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat tttttaaaaa
    15121 agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca gcgtttcgaa
    15181 gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt ttcccacaaa
    15241 acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag tgcagcaaag
    15301 attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg caacgaatgc
    15361 gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat cgatgctgcc
    15421 agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta tgcgcctaca
    15481 aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac aggagctttc
    15541 aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat cttagcaacg
    15601 accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt tgattttaaa
    15661 cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga atcgactatc
    15721 agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg gatgcgggat
    15781 gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac tttagatgat
    15841 gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt gagtgcttgc
    15901 gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc agccggcaaa
    15961 gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct gatgtatcag
    16021 caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt tacagaattg
    16081 gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag cgatactcaa
    16141 aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac agtcaaactt
    16201 gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga tgatcagata
    16261 gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga actacgcagt
    16321 aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca taaaaaacag
    16381 acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga agcgacaaga
    16441 cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct atctgtcact
    16501 cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg actagtgatt
    16561 gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt gcagctttcg
    16621 gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt ttatattaca
    16681 agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga agagggaaca
    16741 gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc tcaagaacaa
    16801 gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc cgaagctgtt
    16861 acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa tgggcaatat
    16921 gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag cacaagagct
    16981 gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag cggtttttac
    17041 aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc ctgatgatat
    17101 cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac aaattgatgt
    17161 ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt agttttaaac
    17221 tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg acagctacat
    17281 gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata cgatcgatat
    17341 gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac gcgatttgca
    17401 tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat gtagaaatcc
    17461 ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa tggcaatgga
    17521 aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat caccgatgga
    17581 agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc atgatgcaga
    17641 tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa cagctatgta
    17701 tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac atggtctgtc
    17761 tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg tcgaaggaag
    17821 acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa gcagcacaag
    17881 agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg atggtgcagg
    17941 aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa aaacagaaat
    18001 cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag cagtgatcct
    18061 tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg cagcagcaag
    18121 aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa tcgttttatg
    18181 cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca tcggcgttac
    18241 cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt ttacccttta
    18301 tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac aaaatcaaat
    18361 cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg cttacttaaa
    18421 gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa gctttgaaga
    18481 agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt ttgaaaacta
    18541 ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga cagcaaccgt
    18601 ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc gacaggcggc
    18661 ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg tgtagaagaa
    18721 gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc tacacctgtt
    18781 acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga agtaggcggt
    18841 gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga gctgagctat
    18901 gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc ttcaacggag
    18961 ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag gaacaggaaa
    19021 acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga aagacaatct
    19081 gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc cagatgtttt
    19141 ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt tgcagacgga
    19201 attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag aagctgaaaa
    19261 aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc ctggagattt
    19321 tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc aatctcgttg
    19381 tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat tgcaagaaga
    19441 gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt taggaaaagc
    19501 agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt atcaatggtt
    19561 cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa aattggtaaa
    19621 atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt tttattatca
    19681 ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac agataaacca
    19741 gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca gttttcaggc
    19801 tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg atgatgatca
    19861 aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg tcatatctat
    19921 tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt cgaatctcag
    19981 caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc caatgacctt
    20041 cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga aaaagacaaa
    20101 aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt gcagttgcgt
    20161 gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa attgattttt
    20221 tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct ggccgctgta
    20281 tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa gataataggc
    20341 ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga tttcgtgcct
    20401 gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa aatttccggt
    20461 ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga gacagcgaaa
    20521 aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg gaaagtcgta
    20581 gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga aaatcctgtc
    20641 gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa tggataagag
    20701 atcattatac gacggattga atcaattaga atcagagttg agaaatacat taacagaact
    20761 tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt tggagatgga
    20821 aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag ctccagctga
    20881 aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt atgaagaagg
    20941 attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc cttgtgcgtt
    21001 ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag gagactggag
    21061 cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta taatcaaaaa
    21121 agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc aattggaaat
    21181 ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct tatcgcaagt
    21241 gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac accccaaaaa
    21301 agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt gttaaaagaa
    21361 ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga tcctggacat
    21421 gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc aggacctaca
    21481 gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt attttatggc
    21541 ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa agaacagaca
    21601 gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac gaatttttta
    21661 gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt ccatgaagaa
    21721 taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc agtaaagggt
    21781 gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga agttgatgaa
    21841 gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga aaaaccaaat
    21901 gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt ctaccgtcag
    21961 taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa tgaggaaatc
    22021 agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa tgaataagag
    22081 gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa aatcattcat
    22141 attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga gtttcgcgga
    22201 catccattag taattgccag acaccccagt gacactggtg ggcgtggagt agtgactaca
    22261 gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca aaaagcgtat
    22321 gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag cgatatttcc
    22381 aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt tagtatcgat
    22441 gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat caaaatcgcc
    22501 cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc gggagtaagt
    22561 tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg aatcacggta
    22621 gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa atttcatggg
    22681 gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg aaaagaccta
    22741 tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta ttctctttat
    22801 cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg aaaatctgtg
    22861 ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct gactcagttg
    22921 cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca tgggaaaaca
    22981 gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt gacattgcca
    23041 gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg ggaggaaatc
    23101 ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct ggacccgatg
    23161 acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa acagctttga
    23221 gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa gtcttgcttt
    23281 ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac aacttgattg
    23341 gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg aaattgtagt
    23401 tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat ttgcgaaagg
    23461 agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc tgtccatgac
    23521 cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg cagacaaaca
    23581 tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta atcgttgcgc
    23641 aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata tcggaaattt
    23701 tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac gtaaagactg
    23761 ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat atagttttgg
    23821 ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa acttctttca
    23881 tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat aaataagaac
    23941 aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga gggcgttcaa
    24001 agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct cttattctga
    24061 ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg aaaaatatga
    24121 ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt tcacaacctc
    24181 atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc atcggacgag
    24241 cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt tttggatcat
    24301 gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca ccttcgaaac
    24361 cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt ttgtcatatg
    24421 caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg tattcacagt
    24481 agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc aatttttcaa
    24541 atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat tctttatctg
    24601 tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg tcagttagac
    24661 tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc catgcatctg
    24721 cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct gggtttgttt
    24781 cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag cctaacgata
    24841 cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa agacgtttcc
    24901 cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct ttacaacgtt
    24961 ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt aactgccaga
    25021 atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat gtaacaacac
    25081 ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac ccttgcaaga
    25141 atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt ttatcgtaat
    25201 tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt ttgatgtcgt
    25261 aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc agtttaggga
    25321 agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa attgcttttt
    25381 gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg aaaggagttt
    25441 gtccatttga agtaaataag gtattgattt catattccaa actttgcata gcatcgaaga
    25501 tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa tcgatccatt
    25561 cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca agcaattcat
    25621 ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca ataatctgtg
    25681 agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca ttgccgattt
    25741 tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc atcggtgtat
    25801 aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca tttgctatat
    25861 gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc agatctcgct
    25921 gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt tggtccttgt
    25981 tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca aaatctcgtt
    26041 tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct agtaatatat
    26101 gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg cttttgattt
    26161 cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt gataatgagc
    26221 cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca tcgaaatcaa
    26281 catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc tgagtaacga
    26341 cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt gttcttgtct
    26401 atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag tattcaatgt
    26461 gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa aaaaggtgaa
    26521 aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa aataattgaa
    26581 taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa ttattcgtag
    26641 atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa gaattggaaa
    26701 agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct cccgtcaaag
    26761 ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt atgggcccaa
    26821 gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg cctacgatgg
    26881 gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag ctcagtgact
    26941 ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat accctaaatg
    27001 caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag gaaatggaaa
    27061 agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa cggtatcccg
    27121 atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt gttgttcagc
    27181 cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg gctacagaat
    27241 tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg ttagtcaccc
    27301 atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat ggtgtgattt
    27361 tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta atagacatgc
    27421 aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt taaaagtttt
    27481 tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc cgtaatgatc
    27541 tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc aagtcaagat
    27601 acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt tttgcttttt
    27661 ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat aggcatctat
    27721 cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga aatcatgata
    27781 attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc caaactcttt
    27841 tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt tatctccatt
    27901 ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt atctgtatat
    27961 tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa agaacaagca
    28021 gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat cgggttacta
    28081 cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc atggatgatc
    28141 agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt tttcgtttgt
    28201 gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat catattgagt
    28261 aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac gcaagttcat
    28321 ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat ttctttaatg
    28381 acaattggcg gcacggctag tatctctact attttatcct accaggagaa agtttcctcc
    28441 ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat tctagcagag
    28501 gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg gagtatctat
    28561 agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga aacacaatat
    28621 caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc acctggtcat
    28681 actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata tggatctagt
    28741 attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag tgtattaggt
    28801 gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt ttttgaaaaa
    28861 gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa taatgaaaaa
    28921 atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt ttattattct
    28981 tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact tccggatgac
    29041 caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc ttccagctat
    29101 tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac actatttgtc
    29161 gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt agcagaagca
    29221 gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca aagaaggatc
    29281 agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat attagggatc
    29341 actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc cgattactgg
    29401 ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta tgtcattacg
    29461 agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa gataataaat
    29521 agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga tttttgtcac
    29581 aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa aaagcagtga
    29641 tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa aacggtttct
    29701 gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg aggttatatg
    29761 aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt ggggaaaatt
    29821 catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc tgtatcacta
    29881 taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag agtatggaaa
    29941 tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat cgagttgctt
    30001 atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac gcacttgctt
    30061 attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc aatttggagt
    30121 ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat ttgccgattg
    30181 aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa ccatctatgg
    30241 tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata acaaaaaaag
    30301 aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc gtaaaaggta
    30361 cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa ggcgtacaaa
    30421 tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt gggatcaagc
    30481 ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta ttttctattt
    30541 atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt gttcaagact
    30601 ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt tctactccat
    30661 catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg ccggaattgg
    30721 acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc
    30781 gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact gtagctattt
    30841 caagtattca gattgaccag catgtactag atagttatga ccgactaccg attggctacg
    30901 taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca aaaggagaaa
    30961 cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa
    31021 agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca ggggatgcag
    31081 gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag caggtcaaat
    31141 tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa gacaaccgaa
    31201 tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag ctggttgctt
    31261 ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa tctatcaaag
    31321 aacaattact agaagttgta atggattata tgattcctca gaagttccat tatgtcgatc
    31381 tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc
    31441 ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt ttcttttagc
    31501 cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat taccagttta
    31561 tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt attggcaaca
    31621 gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg gctattttgt
    31681 ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt tagcgataat
    31741 tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat cgttacttgg
    31801 ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg aaataagaga
    31861 tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt tctttccaac
    31921 aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc tccatccgcc
    31981 tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt ttgtcggttt
    32041 tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg tagtgggacg
    32101 cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt acgtgtacag
    32161 tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta caagctacat
    32221 gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga atatcaaaga
    32281 attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg tatatatgcg
    32341 attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat
    32401 cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt ggtactacat
    32461 cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga tccgatttaa
    32521 gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag ccgtattttt
    32581 gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag ataaactttt
    32641 ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag gaaaatggaa
    32701 gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag aaaaaatcgt
    32761 gatattgatc tatttgatga agggttgctt gattctttag catctgttca attattagtc
    32821 gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg agaggattgg
    32881 agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt atgaaaaaga
    32941 aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt gtgtttttta
    33001 cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc tcttcttcca
    33061 tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct gaaaaacaat
    33121 atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat ccgtctgttc
    33181 tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc gggacacaat
    33241 cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac aagaaagtcg
    33301 tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat tactttaata
    33361 cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa gtaacgcctg
    33421 ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag aatgatacgt
    33481 tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa tctttgaatc
    33541 agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc aatattgggt
    33601 tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa caataccaag
    33661 aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg aataatccat
    33721 tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca gagctcaaac
    33781 agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt cagctggtct
    33841 tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg gttaacgaga
    33901 aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc aaaaagatca
    33961 aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat caagcgggta
    34021 ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg gctgcagatc
    34081 aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat catcttgacg
    34141 atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa ttaaaataaa
    34201 gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg aataaaagat
    34261 aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac aatatgaata
    34321 tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg atttaacttc
    34381 tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag aatatgccaa
    34441 accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca aacatggtgc
    34501 tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt ttatggtaac
    34561 aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt gggggcaagt
    34621 catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat
    34681 tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt atgaaataaa
    34741 ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg
    34801 tcctggagat atggtcactc catatattga atacaaacga atgaatggca gtaaatattt
    34861 actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag tattagagaa
    34921 tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc aagaagaagt
    34981 aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg cttttgcttt
    35041 agatactggt acagcaggag acactccagg aatgacacca aaagaagcag attcgatttt
    35101 aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata aaaaattatt
    35161 gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata cagtgatttc
    35221 tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc cgtctgtagc
    35281 cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg aagatgatta
    35341 tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg aaacagttga
    35401 aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata tcggaaaaaa
    35461 gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt cttatctata
    35521 atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc ggaagaaagc
    35581 atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc attcaactat
    35641 gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag taacctttta
    35701 ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt gatcaattca
    35761 gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat cagcggagtg
    35821 agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt catgccagaa
    35881 gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt ttcagcactc
    35941 atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt cgactatcct
    36001 gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga tagaattggt
    36061 acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc aatcgctttg
    36121 ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga attttatgaa
    36181 caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt aagcagttta
    36241 ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg tacgatgaaa
    36301 ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac aacaggttat
    36361 ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac gctgatcaaa
    36421 ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca ttatcatcat
    36481 gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc taagagaaat
    36541 ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt tgaagaattg
    36601 acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt aaaaattggt
    36661 ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc catgcgtatc
    36721 gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta tttggaaagt
    36781 ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct ttttgaagga
    36841 aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc gaaatcagct
    36901 aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga acaattgaaa
    36961 aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc agttgacaaa
    37021 gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc acgacccaag
    37081 aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg aacctatttt
    37141 gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata cgcttgaaga
    37201 aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct taagtggagg
    37261 cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc ctgatgatgg
    37321 ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac ttcatgaatt
    37381 gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt
    37441 ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa caattatgtt
    37501 cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc
    37561 aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc ttccagaaga
    37621 aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt
    37681 agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga aaaaaatcaa
    37741 tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt ctccagattt
    37801 caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt tgaatgtctc
    37861 agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag gagatatctc
    37921 tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat caaaaatagt
    37981 cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat tgcttacctt
    38041 actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta ttttttatac
    38101 aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag tatctttttc
    38161 ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta agtcaaaacg
    38221 ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc
    38281 actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt
    38341 tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta aagcgtggat
    38401 caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat ctgcttggaa
    38461 ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga taagatttgt
    38521 cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt ctaatatctt
    38581 ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca gattctccat
    38641 ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc gaagctgctc
    38701 ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc tccgatcttt
    38761 gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta gaatgataat
    38821 atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg tttggatgta
    38881 aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta cataaaaacg
    38941 gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc ttataacact
    39001 agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag ttgcataaag
    39061 cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc attttttata
    39121 gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga taataaataa
    39181 atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa tatcttctga
    39241 tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg agtattcaag
    39301 acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag cgataaaaac
    39361 agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac taatcaatgg
    39421 tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat aaggagatga
    39481 gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca cagccaatga
    39541 gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct tttcgcctag
    39601 atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt ccaacggttt
    39661 ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt
    39721 gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc acgaatcttt
    39781 tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga aataagcaat
    39841 tcctgttaaa aataacaatg ataatataa
//
LOCUS       DAYYOA010000014        38540 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000014 DAYYOA010000000
VERSION     DAYYOA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.014"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..622
                     /locus_tag="ACHBKA_000673"
     CDS             <1..622
                     /locus_tag="ACHBKA_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444388.1"
                     /translation="FVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLLT
                     VAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKTY
                     QDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRIT
                     AGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1072..2121
                     /gene="trpX"
                     /locus_tag="ACHBKA_000674"
     CDS             1072..2121
                     /gene="trpX"
                     /locus_tag="ACHBKA_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9444389.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2118..2999
                     /locus_tag="ACHBKA_000675"
     CDS             2118..2999
                     /locus_tag="ACHBKA_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444390.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3002..3772
                     /locus_tag="ACHBKA_000676"
     CDS             3002..3772
                     /locus_tag="ACHBKA_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444391.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4051..4905
                     /gene="aroE"
                     /locus_tag="ACHBKA_000677"
     CDS             4051..4905
                     /gene="aroE"
                     /locus_tag="ACHBKA_000677"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM9444392.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5083..6246
                     /locus_tag="ACHBKA_000678"
     CDS             5083..6246
                     /locus_tag="ACHBKA_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9444393.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6363..6773
                     /locus_tag="ACHBKA_000679"
     CDS             6363..6773
                     /locus_tag="ACHBKA_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9444394.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6947..9175
                     /gene="topB"
                     /locus_tag="ACHBKA_000680"
     CDS             6947..9175
                     /gene="topB"
                     /locus_tag="ACHBKA_000680"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9444395.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9346..9630
                     /locus_tag="ACHBKA_000681"
     CDS             9346..9630
                     /locus_tag="ACHBKA_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444396.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9790..10026
                     /locus_tag="ACHBKA_000682"
     CDS             9790..10026
                     /locus_tag="ACHBKA_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9444397.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10023..10421
                     /locus_tag="ACHBKA_000683"
     CDS             10023..10421
                     /locus_tag="ACHBKA_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9444398.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11088..12032)
                     /locus_tag="ACHBKA_000684"
     CDS             complement(11088..12032)
                     /locus_tag="ACHBKA_000684"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9444399.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12328..13272)
                     /locus_tag="ACHBKA_000685"
     CDS             complement(12328..13272)
                     /locus_tag="ACHBKA_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9444400.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13492..14706
                     /locus_tag="ACHBKA_000686"
     CDS             13492..14706
                     /locus_tag="ACHBKA_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9444401.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14728..16743
                     /locus_tag="ACHBKA_000687"
     CDS             14728..16743
                     /locus_tag="ACHBKA_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM9444402.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16826..18199)
                     /locus_tag="ACHBKA_000688"
     CDS             complement(16826..18199)
                     /locus_tag="ACHBKA_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9444403.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18225..18509)
                     /locus_tag="ACHBKA_000689"
     CDS             complement(18225..18509)
                     /locus_tag="ACHBKA_000689"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9444404.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18511..20526)
                     /locus_tag="ACHBKA_000690"
     CDS             complement(18511..20526)
                     /locus_tag="ACHBKA_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9444405.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20705..21010)
                     /locus_tag="ACHBKA_000691"
     CDS             complement(20705..21010)
                     /locus_tag="ACHBKA_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM9444406.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGNNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21099..22130)
                     /locus_tag="ACHBKA_000692"
     CDS             complement(21099..22130)
                     /locus_tag="ACHBKA_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9444407.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22337..23212
                     /locus_tag="ACHBKA_000693"
     CDS             22337..23212
                     /locus_tag="ACHBKA_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9444408.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23364..24206)
                     /locus_tag="ACHBKA_000694"
     CDS             complement(23364..24206)
                     /locus_tag="ACHBKA_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9444409.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24298..26175)
                     /locus_tag="ACHBKA_000695"
     CDS             complement(24298..26175)
                     /locus_tag="ACHBKA_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9444410.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26172..27593)
                     /locus_tag="ACHBKA_000696"
     CDS             complement(26172..27593)
                     /locus_tag="ACHBKA_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9444411.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27596..30985)
                     /locus_tag="ACHBKA_000697"
     CDS             complement(27596..30985)
                     /locus_tag="ACHBKA_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9444412.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31242..32633
                     /locus_tag="ACHBKA_000698"
     CDS             31242..32633
                     /locus_tag="ACHBKA_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444413.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32706..33353)
                     /locus_tag="ACHBKA_000699"
     CDS             complement(32706..33353)
                     /locus_tag="ACHBKA_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9444414.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33361..34047)
                     /locus_tag="ACHBKA_000700"
     CDS             complement(33361..34047)
                     /locus_tag="ACHBKA_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444415.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34053..35126)
                     /locus_tag="ACHBKA_000701"
     CDS             complement(34053..35126)
                     /locus_tag="ACHBKA_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444416.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35348..36022
                     /locus_tag="ACHBKA_000702"
     CDS             35348..36022
                     /locus_tag="ACHBKA_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9444417.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36027..37367
                     /locus_tag="ACHBKA_000703"
     CDS             36027..37367
                     /locus_tag="ACHBKA_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9444418.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37516..38502)
                     /locus_tag="ACHBKA_000704"
     CDS             complement(37516..38502)
                     /locus_tag="ACHBKA_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM9444419.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11848 a         7042 c         7301 g        12349 t
ORIGIN      
        1 gtttgttatt tgggaagtag ttaaatcgag agtacaagaa atgaatgaac atttctcagt
       61 gtcattcgca gaaaatccaa gtaagtattt tcgacgtctt gtttttctgc tcatcttatc
      121 attgctgtta acagttgcaa tgtttttatt gccggaaaaa atccgagaag agcaagtaaa
      181 tagtctcgtt tattcgtcca taccaaccag caaattgaaa ccttttgatt acagcaaagc
      241 tgatcagaaa atcaaacaaa gccgggcgat atcggtgatg ttcagcaaac cgaacgggaa
      301 aacctatcag gacatgcttg atgtttttaa tgatcctgag cttatggaag aattgaatcg
      361 accgatcttt tattatccga tcgtttatga tgtacatgaa ctggaacaaa aatataacat
      421 acgaacggat gaagtgacat ttattttctt tgatggagga aaagaagcaa atcggataac
      481 tgcgggtaag ggaggaataa ctgattttga taaggagttg atccctgaac tgaatcgact
      541 tccgttagcc aatatcaaac aattagaaga agaattggat agaacgggag taacttccca
      601 aaattccgag attgctaatt gagacattgg cgcgatgaaa cggtgggtct atatagcgct
      661 atttttaatt atcagaacaa gtgatgtttg atcttcaaaa taacaaataa aaaattaaaa
      721 aatatagttg acgaaattca aaaattcgat tagacttaaa tcaattctta aggaaagggt
      781 gacctgtaat gaacttatca aaacgtattt cagcagaaaa caaaaaaaat tggcgcagct
      841 ggcgtagata agttgtatta atggtgagac tttcccatag atacaacttt ttagagtgca
      901 tttctaaagc ttgtatctat gctagacatg aacattgact gccgcataga ttagaatcta
      961 tgcggttttt tgtgtaaaga caatgaccgc tttggccatt gtcttttttt gtagactgga
     1021 tacttgcctg attataataa ataaaaagat tgactttgca ggaggaaaaa aatgaaaaac
     1081 aaacgattaa taacagttgt agtaattatt ttagtttatt tagcaggtgc atttatttat
     1141 ggaaaagcct caggcgaaaa agaaagtact tcgacgaacc agacaaaaaa aacaacagtt
     1201 gttggggtat tgcaatacgt gagccatccg gcattagatg agatttatcg aggaatcaaa
     1261 gatggtctga aagaagaagg attaaaagaa ggagaaaatc ttgagatcaa atttcaaaat
     1321 ggacaagccg atcagagcaa gctagccaca atgagtcaac agctagtaca atctgatcca
     1381 gatgccttga ttggaatcgc aacacctgcc gctcaatcat tagcaaacac gacttcatcg
     1441 atcccagttg tgttaggtgc tgtaacggat cctgtggggg ctggattagt cgcttcttta
     1501 gagaaacctg gtggtaatgt aacaggtgtt tctgatcagc cgcctgttac ttcacaaatc
     1561 aagcttgctt ctgatttgtt gccaaatgca aaaaaagtcg gaatcttgta tgcttcttca
     1621 gaggataatt caaaatacca agtagcacaa gcagaagaag ctgcaaaaaa agaagggtta
     1681 gaatcagtaa aatatgcggt gccttccact aatgagattg cacaaacggt tcaagtgatg
     1741 agccgacaag tcgattttat ctatgttcca ttagataata ccattgccaa tgcgatgcaa
     1801 acagtggtaa aagaagcaaa caaagcaaat atccccgtca ttccatctgt ggatacaatg
     1861 gtggaacaag gtggactagc aacaatcggg atcaatcaat atcaattagg gcttcaaagc
     1921 ggtaagatgg cggcagaact tgcgagtgga aaagaaaaac cggaaacgac gcctgtttat
     1981 acatttgatc aaggggatac agtgatcaat caatctcaag ctgatcgctt aggaatcatt
     2041 gttccccaat cgatgaagga aaaagccaaa atcattacag atgactcgca gcaagaaaca
     2101 tctaaggagg atgacaaatg atcgtatcag ctatttcaca aggcatgctt tgggcaatat
     2161 taggattagg gattttcatg acttatcgga tcttagactt tcctgatatg acgacagaag
     2221 ggtccttccc attaggtgct gctgtctgtg tgacagctat tactcatggg gtagcgccga
     2281 ttgtagctac attattaggc gtaggtgctg gcatgatagc aggactcgtt acggggttac
     2341 tttatacaaa aggaaagata ccggttattt tagcagggat tttagtgatg tcaggattga
     2401 actcagttat tttgtttgtg atgcgaacac caaatctttc tttattgaat catccgattc
     2461 tacaagacag cttccatcgc tttgggttac ctgattactt tgacacagtc cttcttggct
     2521 tcatctcttt gggcatcttg attgctgtac tgctattctt cttcaataca gatttgggtc
     2581 aaggctatat tgcgacagga gacaacgaaa cgatggctcg gtcgttaggg ataaaaaccg
     2641 atcgtatgaa aattttaggt ttaactttgt cgaacggtgt cattgcatta tccggtgcgc
     2701 tgattgccca gaacgacgga tatgcagatg taaataaagg gattggcgta atcgtcatcg
     2761 gtcttgcttc catcatcatc ggtgaagtga tcttccaaga attgacgcta gcagaaagat
     2821 tgattgctat tgttgtcggc agtatcattt atcagctatt gattttagta gtaatcaaac
     2881 tagggtttga cacgacttat ctgaagttgt tctccgctat catcttagct atttgtctga
     2941 tgatcccgca attgaagaaa gcactgaact taaaaacggg ttttgagaag gaggcatagt
     3001 aatgactgat atattgacaa tcaaaaatgg cagaaaagtc gtaaacaatg gaatgaatga
     3061 agagaaagta ctattggatg aaattaacct tacaatcaga gaaggggact tcatcactgt
     3121 ccttggcggg aatggtgctg gaaaaagcac actgttcaat acgatcgctg gtacgcttca
     3181 actcagtcaa ggatcgatct cttttaaaga tcagctgatc acaaaagatt cagaagaaaa
     3241 aagagcacga ttcctttctc gagttttcca agaccccaag atgggaacgg ctcctcggat
     3301 gacagtagca gaaaatttgc tacttgcaca aaaaagaggt caaaaacgga aattccgttt
     3361 acgtaacttg aaaaatcaaa aagaagattt tttcagacta tgtcaagaag tcggtaatgg
     3421 cctagagcaa cacttggata ctccagcggg tgaattatct ggtggtcaac gccaagcatt
     3481 aagcttgctg atggcgacga tcacagctcc tgagttacta ttattagatg aacatactgc
     3541 agctttagat cctaaaacag ctaaagcatt aatgcgatta acaaatcagc ggatcaatga
     3601 acaaaaattg acttgtctca tgatcacgca tcgaatggaa gacgccttga aatacggaaa
     3661 tcgtctgatc gtactccaaa aagggaaaat cgttcaagat atttctgaaa aagaaaagca
     3721 acatttgaca atgggcgacc ttttgcagtt ctttgaagaa gtagtggatt gatttcgaca
     3781 gaaaaattcc tttcagtaga aggacttgaa aactcattga catcttcctt tgattcatcc
     3841 tatacttaaa acaataaaca attaaacata tttgggtgtt ttgtaggatc acgtagtcaa
     3901 tgattttaac aattaaatag aatattcttt gctctgacaa gtaaacgatg agaaaagaat
     3961 ttgctacttt gccacctaga cgtttcgtac tttttcgtat caaacgctaa gtggcttttt
     4021 ttattgaagt aagaacaaag gaggaataag atgatttctg gacaaaccaa attagctggg
     4081 ctctttgctg atccggcttc tcacagttta tccccattga tgcacaatac tgcttttgaa
     4141 gcaaacaaca tcgatgcggt gtatttagct ttccaagtaa atccaatgaa tttgaagcaa
     4201 gcagtcgaaa gtattcgaac ttttgatatg ttaggtgtaa atctttcgat gccgaataaa
     4261 acagcggtta ttccttatct ggatgaggta agccaggaag cgcaattgat cggagcagtg
     4321 aatacgatcg tgcacgaaaa gggtcgtttg attggctata ataccgatgg tgcgggcttc
     4381 atgcaatccg tgcaagaagc tgggatttcg atcaaaaaga aaaaaatgac tctactggga
     4441 gctggaggtg cagcaaaggc agtcactgtt caagctgcgt tagacggcgc tgaggaaatc
     4501 gtagtttata aacgaaacaa ccaaacattc gctcaagtaa aagaaacatt cgaacaactc
     4561 agctatcgaa cttcttgtaa gataaaagtt cgtgactttg ccgatacgca tcaattaaga
     4621 aaagacttga aagaaagctg tcttctagtc aatgcaacag atatcgggat gggaaagaaa
     4681 atcaatcagt caccgctctt agatataagc atgatgcatg agaatctagc ggtttttgat
     4741 ttgatttatg cacctagaga aacacgttta ttaaaagaag caaaaaaagt aggagcaaaa
     4801 ggatacaatg gacttggcat gttgatctac caaggagcaa tcgcgtttga attatggaca
     4861 aaggaaaaga tgccgatcca tgtgattgaa aagctgtttg attaaaaaag gataatagat
     4921 aaagatcttt tattctcgca taaacggtct caagaaaagt aatcctttat gtatcgaagc
     4981 atttatttcg ttatttttta ttaaattatc aaaaaatgat aggcttttgt cgattggtta
     5041 tcgtatcctt tatttatcag taagaaacga ggaggaacca caatgagtcg gatggatcgc
     5101 tataaggaca tccataagaa ggcgaaacct ctgaagcggg atacggggag tatttttgct
     5161 agaagagaac gaaaacgaac gcgtatggaa aatgaggaag atgctacccg tatttatgaa
     5221 agagagcagc attcatttga acatttgaac caatacgagc aacagatata cagagatgca
     5281 ccaagagcag aagaacaaca aaaaaataaa aaatttttca atcggcaaaa gaagaacaaa
     5341 ccgttaaaac ctcgaagaaa gcgatcgtgg aaaaaaatca ttctatcgat tttgcttgtt
     5401 ttgatccttt attcaggtat ctcatttttt attggtaaat atgtagctga acatgacaca
     5461 agcttagagg cagcgacttc agaaaccttt catgggcaaa aagcgacgaa tggtgcgcat
     5521 aatattttga tccttggaag tgatacacgc ggagaagatg ccgggcgagc cgatacgatc
     5581 atggtcttac agttggatgg tccttcccat aagccgaaac tgatttcttt tatgcgtgac
     5641 agcttcgtta cgatcccagg ggtaggacaa aataagatca attcagctta tgcatacgga
     5701 ggagccgatc tagtgcgtca aactttagta gaaaattttg gaatcgattg tcaatactat
     5761 gcgaaagtcg atttcaaatc gtttgaaaaa gtgatcgatg ccttattcat gaacggagtg
     5821 aagatcgatg cagaaaaaga tctgaatctg gatggtgtag atattaaaaa aggggtccag
     5881 aagatggatg gacatgttct tttgcagtac gcacgtttcc gaatggatga acaaggagac
     5941 tttggccgtg tcagacgcca acagcaagta atgaacgcta ttttcagcca attgaagaat
     6001 ccattgaatc tgatccgtgc accttatgca gctggtaaag ctttaggata tacatccaca
     6061 gatgtcactg cttggttctt gacgaaaaac ctcttttcga ttgcacgagg agctggtggc
     6121 attgatcgaa taagtgtgcc agtagacggt tcgtggaatt atgggcaaag tgattatgct
     6181 ggcagtattc ttgaaattga taatcaaatg aatcaatctg ctattacaga ttttttgaat
     6241 aaatgatcat aaaaaagatc aagaagaagc tgcgcaaggc ttttgtcatg aatcaatcaa
     6301 acgattcact cgtaaaaaac gctttcttat ggtagattta accaagagag gagctgttta
     6361 cattggatat ctatctgaac ttcaaaaatg agtcgcgtga agccatcgct ttttatgaaa
     6421 aagtttttca gactgaagca tcaggtatca ccacatacgg tgaaatgcct ggaatagaac
     6481 aaatgaactt gccagaagaa gcaaaaaatc taatcacgaa tgcacggctg aacatttatg
     6541 atacattagt tatgtttgca gatacgccag aaggaattgg tcctgaatta gttgtgggaa
     6601 ataatgtttc tttagtcatc cagacaagaa atgaagaaga aacgaagcgc ctatttgaaa
     6661 gcttagctga gggtggtaag attgagatgc cactggatgc tgttccgtgg gctcagttgt
     6721 atgggatggt aactgatcga tttggcatca actggcagat taaccaagag tgacagctaa
     6781 agatgaatat tttctggaaa catcaagcag caaacggatc atcgtttgtt tgcttgatgt
     6841 ttttgtgtct atttcgacta tacggtatct tttttttatt gccagatgcg aaaatatgtt
     6901 ccataataga gaagtccgca aaaaattaag taacggaggt cagaagatga agacggtgat
     6961 actagctgaa aaaccttcac aagcaaaagc ctatgccgaa accttccaac agcaaaagcg
     7021 gcatgaaggg tattatgaaa tcagcgatcc attgtttgct ggtgaagtaa ccatcactta
     7081 tggttttgga catttggtag atatggttgc tccaggagct tatgaagagc gttgggcaaa
     7141 atggtcttta gaaaatttgc ctattttccc agaaaccttc cgttacgaag ttccaatgga
     7201 caaacgtgca caattttcga tcgtaaaacg agaattgcag tcagccgata ccatcattat
     7261 tgcaacagat ggtgatcgag aaggtgaagc catcgcgtgg tcgattattc gacaagcaaa
     7321 cgcattttct aaggaaaaga actatttacg tttatggatc aattctcttg aaaaagaagc
     7381 aatctatgaa ggttttaaga atctccaacc tggaaagaac tattttccaa aatataaaga
     7441 agcgcaagca aggcagaacg ctgattggct gattggaatg aatggaagtc cattatatag
     7501 cttgctactt caacgaaaag ggattgatgg cagtttttct cttggtcgtg tacaaacacc
     7561 gacactctat atgatttatc agctgcaaga agaaatcaaa cacttcaaaa aagaaacgta
     7621 ctttgaagga gaaggtttaa ttacgacaaa caaagggcag ttttctggaa aaatcgtacc
     7681 aaaaaaagca tttaaaaccc aagaagagtt aacgaaggaa atagaaaaac ttggagcgca
     7741 tttaggtaag caaaacggac ggattcttga agttacgaaa aaggaaaaga ggttgcatag
     7801 tccacgttta ttttcgctat ctagtttgca gacaaagatg aatcagctaa tgaaagcaag
     7861 tgccaaagct acacttgatg ctgctcaagg attgtacgaa agcaagtttc taagctatcc
     7921 aagaacggat tccttttaca ttacagaaaa tgagcatcag tatctggtta aacatctgca
     7981 aaattataaa aaatttttag gaatagaaga agtacagaca actgagatca aagcaaagaa
     8041 acgatatgtc gatgcgaaaa aagttcaaga acatcatgca attattccga caaagacgat
     8101 tccgactcct gcacgctttg cgaagctttc caaattacag caagcaatct atttgcaagt
     8161 tttgcgcact actgtggcta tgtttgctgc agattatatc tacgaagaga cgaccgtaat
     8221 gacgggcgta cagcaattaa agctaaaaag catcggaaag atcccattac agcaaggttg
     8281 gcaggaaatc ttacgcatca agtcaaaaaa gaaagaagct cagactcttc ctcctatcgt
     8341 agaaaatgaa gaagcagaag tagaactgaa aagtatggag aaagaaacac aacctccaaa
     8401 accctatacc gaaggaacgt tgatcacagc aatgaaaact gcgggaaaaa cgctggataa
     8461 tgatgaagcg caagaaattc tcaaagaagt agaaggaata gggacagaag ctacacgagc
     8521 caatatcatt gaaaatctta agcagaagca ttacatcgaa gtcaataaaa acgaaataac
     8581 tgttacgcca aaaggaatca ctctttgtaa ggcagtagcc aatgaaccat tactgacgag
     8641 cgcagaaatg actgcgcgct gggaagggta cttgagaaaa attggtaaac aagaggggac
     8701 accatctgtt tttttagaga atatcaaaaa attcatccta catttattgg cagaagttcc
     8761 taatcaaatc gacaaggtag acttttcaga tgagattagc gggacacgtc agataaaagc
     8821 gatggagaag aaaaacgatc aattaggaag atgtccaaaa tgtaaaaagg gaatagtcat
     8881 gctttatcct aaaatcgcta catgcttgaa tccggaatgc gacttcaaat tgtggccaac
     8941 agtagctaaa aagaagctga ctaaaacgaa tctcagagat ttgctttcaa aaggaaagac
     9001 aagtaaagtt gtcaaaggtt ttactggtaa aaaagggaaa ttcgatgcag ttttagtatt
     9061 aaaagaagat atgacaatcg gtttttcttt tccatggatc gaaaacagtg gaacaaaagc
     9121 agttgatgga aacaaagaaa catcaagaaa aaataatgaa aacgaagtag agtaaatgaa
     9181 aacaatgaaa tagcggaata tttattccga aaagtgagaa tataaatacc aataaaacta
     9241 aaaaatatca gtgaatcaag caaaaacgtt ggggtaggct gattttatat agatagcaag
     9301 gagttttttt atttgaaaaa agtgcattca tttttaaatt atcttatggg agcaatcacg
     9361 attgtggctg tgtaccaagc atttctaaag tatcctgatt acgggacgaa gttgttgtca
     9421 ttgacgcaca atcaaacagc atattcacca acgttgattt tatcgacttt gactgcagga
     9481 gctaaaagcg ctacttattt ttttcataaa acgacaagca tgctaaagcg aaagaaacag
     9541 cgaaaacaat tgttggtggt ggtagcggct atgatcatta tttattttct accagttatt
     9601 acacaagtat ttcatcaata ttttggttaa gaatacgaga gtttgtgaca aaagtcatgt
     9661 cacaaacttt tttctttgtc attttttcgt aagaaaaagc aaaataaaag tatatacatt
     9721 gattaaaagt atatacttgg atataatgta cttactatag aatgagaatg gtatagaggg
     9781 tggcgaaata tgaaaaaaat gaaaaagtat aaaattagaa aatctggcaa tagcgatgta
     9841 acaactatcc caccagaagt aaaagaattc atgggtgtgc agacaggtga cgcaatcagt
     9901 tatgtttttc aatctgatgg ttctgtacga atgatcaaag cacaagaaga acctgatatt
     9961 gattcattag ttgattcgat catgaatcaa tatgaagacg cgttaaaaga tttggttgat
    10021 ttatgaggta tctttcgact aaagaaatta tcaaaatcaa cgcgaaagtt attttacgtc
    10081 actcgccagg cgaaatgatc ggtgtaaaag atgcaaatgc attagatatg gccgtcaaac
    10141 agccttcaca agcagtattc aatcaagaac tctatcctga aatctatgaa aaagcggcga
    10201 ttttagcaat taatttagct aaaaaacatc cttttcataa tggcaataaa cgaactgctc
    10261 ttgttgccat gttgttgttt cttcaattga ataattgtcc agttgctttt tctcgtcaag
    10321 aagctgttga ttttatcata atgattacca ctagttcatt ggatttcgat tcactaaagt
    10381 ccaagataac cagttattta cgacaaaccg caataaaatg aacgaattaa aaactttatt
    10441 tctcaattag atcagcatgt cattaaggaa tatgctgatt tttttatgtt taatcataca
    10501 aaatagtttt ttcataaaaa tatggaaaat atggattaat tttgaaataa ttgatttaaa
    10561 aggaatacaa aagagtgaat agtaggaaat agtggtttag aactagtggg ctgatttttt
    10621 atatatttta gtttacataa tgtgtattat acaaagtaga tttaaaaaat gataaacctc
    10681 tctcgaacgg ttttttatca aaaatcgtca tttttccatc aaaatctgct tatgcaaaat
    10741 tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt ttaattgaat gataaaaacc
    10801 agtttcttta ttcagttaat caaatatgaa cgaattagat atgaatcatt gtatttgccg
    10861 ttgcggaagg attttttaat taaccacaga taaacattga taacgattcg cttattgtaa
    10921 tcaacagatt aaatttcata tgttcatatg tacggataca ttttcggacc gattaatttt
    10981 aaccattctg tttatttgct tacctattag atatcaataa aaaactgctt aacagctatt
    11041 tcagctgtta agcagtttta ggatttttag ttcatattca ttgtttctta taatacagtg
    11101 ttcaatactt ctcttaaagc acttgttgat ttgctgaaca ttgctttttc ttcttcgttg
    11161 attgataatt caacaacttc acgaacacca ttttcgtcaa cgattgatgg cacacctgta
    11221 aagatatctt tttcgtcgta ttcaccagtt aaataagcag atacaggcaa tacagcttgt
    11281 tcgttattca agatagcttt tacgatacgt gcagtactca taccaatacc atagtaagtt
    11341 gcttttttac gatcaatgat ctcataagca gcattacgga ctttatcagc aatcacatct
    11401 aattcatctt gggcaatacg atggtctttt tctacaattt caaacactgg tttaccaccg
    11461 acagttgtat gtgaccatgc agcaacttct gaatcaccat gttcacctaa gatatagccg
    11521 tggacgctgc gtgggtcaac ttttaatttc aatgcgattt ctttacggaa acgagttgta
    11581 tccaatgttg ttccagtacc gataacacgt gatgttggca gaccagattc gttccaagcg
    11641 atatatgtca aaatatctac tgggtttgaa gcaacaacga taataccgtc gaatcctgat
    11701 cccatgattt cttttacgat ttggcgcatg attgacgcat ttgttttaac taaatctaaa
    11761 cgagattgac caggtttttg gttgatacca gctgtgataa caacgatatc agcatcttta
    11821 cattcttcat agccacctga ccatacagcc acattttcat cgccccaagc cataccatct
    11881 aaaaggtcca atgcttcccc ttctgctttt tcttgattca catcgatcaa tactaattca
    11941 ttagagattc cttggttgat cattgcataa gcgatacttg ttccaacaaa acctgtaccg
    12001 acgattacta ctttacgact tgtttttttc atgaataaac agctcctttt cataactatt
    12061 tgttactttc atgaaaagta tagtcttgtt tattcatttt atcaacatgt taattataat
    12121 agttgttatt ataatttttg atggaaacga attcctgaat acgcataaca gataaatgac
    12181 aaatgtttac acctatatcc cctgataata caaccaaaag tttagaactg aaaataaatc
    12241 caaaaaaatt cgctgcaacg atataataag acagcaatcg cacctaaaac aattttcctt
    12301 catatgcttt agtgaaagat tttcttctta atgtttattt ctccattcat tgggagtaca
    12361 tgttgaatac ttcttgaata atttgaataa agaagtcgtt tgtttatatc cgatacgatt
    12421 agcgatttct tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt
    12481 tttctttaga accagttctt gaaaggaagc atcaaaatac attttcaatt tattactaag
    12541 atagaccgga ttgtagccaa aatgagctgc tgtttctttt aacgtacaag aagtaaaatg
    12601 cttatcgata taatctaata ctctaagcat ctcattttct gcgcatccat ctgctgatga
    12661 aaagctttgt tgttctaata aatgagtcaa ttcgatcagt aatagtgatg tatagagatt
    12721 taacgaacgg atcttatgac tgtatgtatc aaaataaatc aacagcatat tttcaatcaa
    12781 atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat gttccccttt
    12841 ttcttttgat gcatttatca aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt
    12901 caatatctct gtagataagg tttcttcttt taataagatg ttgattaaaa tatcctcttg
    12961 ccctgtgtat tcgattcttt gagtagcttc tttatcaatg atcaccacgt cactttcttc
    13021 catctctatc ctttctccat taataatttg taaacatgta ccgctataca tataattgat
    13081 ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt tactgatatg
    13141 aatatttccc tgttgaaata gatcccctcg gtggattttt ggcggtgtct gaagatggta
    13201 agctaagttt aaatcattga tattcattcc ttgttcacgc tgcagagttt cgattgattc
    13261 ttcgttacgc aaaaaaatgt ctaattcagc tagttccaat tgactccctc ctttttgtaa
    13321 gcggtttctt tcgcttatta gttcacgctg tactgctatt atatgcaaag ctatttttgg
    13381 tttcataaag actttctatg gttgggacat gaacttcctg tgattccgct attgagagaa
    13441 taaccacttc atactatatt tttgataagc gcttacaaaa atgaaaggaa catgcttatg
    13501 aataaaaaga tgatgaaatt ttctctgctg tcagtctctc ttattttgac ttctgctggt
    13561 gcagtcacgt ctgcaattcc cagtatgtca gctacttatg ccacgcgttc tttatctgct
    13621 gttgaattat taacgactgc tccctctttt atggtcacta tttttgtttt gattagtagt
    13681 tttattgcta ggaaaatcgg acaaaaacaa actgtgatca tcggattagt gattgcatcg
    13741 atttgtggta tcctccccct cttctcacaa agttacagta ttgtcttagc ttcaagagct
    13801 ggactaggta ttggctttgg attgatcaat tctttggcag tcagcatgat tgctgccttt
    13861 tatcacggag atgaacgagc ctctatgatt ggttttcaaa gtgcatttca aggatttggt
    13921 gcagctttaa tgacatttgt tgcaggacag ctagtacagt tcggttggca atataccttt
    13981 ttagtctacg ccatcagctt acctattctt gtcttgtttg ttttgtttgt tcctacacct
    14041 gaaacaactg aacttcaatc gcatggcaca gccccaacaa gacaagtaat caaaacgaat
    14101 cctaaaatga ttagttatgg tatttttctc ttgctggtca tcgtggtata taatgctgta
    14161 tcgattaaat tagccacagt cctgacgact tattctctag gaaacgaaac taatgcagcg
    14221 aatatcttaa caatcatgca attggcaagt atggctgccg gagtttgttt tggctggctt
    14281 cagacaaaag cacatcgcta tatgcttaca ctttctttac tactgatggg aatcggcttt
    14341 gtattgattg ccactgtgat aaacctgtat gtgattgggt ttggtgcgat cctaacagga
    14401 atttcttttt cgttattcat tccttactta ttcaaccaag taagtatcca agcgcccaag
    14461 tacgcggaga gtttcaatac ttcaatctta ttggttggcg ctaatttagg tactttcatt
    14521 gcaccatatg gtttgctctt tctctctttt gcttctcttg ggagtaaaac acttgctgtt
    14581 ttcgtcaatg gcagtatctt gctgttgatt agcgcgctta ttagccttct agtagtacac
    14641 aggacgaata gaaagacagt gataagagta gatgaagaaa taaaagaaca attgacaaca
    14701 aaataaattt gatggaggtc gtgtaaaatg acaaatgaaa aacataaaga tagatggctt
    14761 tggtatcctg gagactttga gatccgccat ggactgctac aaaatttcca acgggaagaa
    14821 cgaggttttg actggccagc gtactggtac atggatgatt gccatcggaa cgtcaagttt
    14881 aagagatatt actttcttga tcaaccttcc atgtttaaag tgacgatcca aggagttggg
    14941 tatgtagaaa ttaatggaca aaaacatcca tgtggaaaat ggctgacttg tccagctggt
    15001 aaagcaaaaa ttcgtatctt tgtaggacat acatcagggc taccagcgat gtttatcgag
    15061 ggagacgaag taaaatcaga tattggctgg acagcaagta attttattga agaatatcca
    15121 gctggctgga gtccgcttta tgtcgatatc gcaaaagatc caaatcaaat ctattatcaa
    15181 aaagaatgcg tccatccagt cactgaaacg gagattggcg gaggaatctt attcgatttt
    15241 gggcgagcag tgaatggctg tgtagaacta atatctctgg atgaccatat cgaatctatc
    15301 actctatgtt atggagagtc cgatgtagaa gctatggata tcgattattg ctactataag
    15361 caaataggaa tgacaagaaa aatgaaagct cgtaaacgcg cttttcgcta tttgtttata
    15421 ccagaggttt ctactggaat ggttcaactt caagtttttc atgaattttt atctctttcg
    15481 aaacaagctg ctttttcaag cgattcttct ttactgaatc agatctggtc agtatcagaa
    15541 gaagccttca agttatgtag tgatttgttt tttatcgacg gtatcaaacg agatcgctgg
    15601 atttggtctg gagatgcgta tcaatcattc atgatcaatc aatatctttt ttttgacgag
    15661 gaaatcaata aacgaacgat attagcatta agaggacaaa atgaaatcaa gcagcatatg
    15721 aatactatcg ttgactattc gatcttgtgg ttgattggta tcgaaaacca ctatatgatg
    15781 acgaaagact atgaattttt acgcatcgtc taccctaaga tgcaatcatt aatgagatac
    15841 ttgatggagc aaacgaatga attagggttt atttatggga gagcaaaaga ttggatattt
    15901 attgattggt ctgaaatgga taaagaagga acgcttgctg cagaacaagt cttattactg
    15961 aaagcctatc attccatgat cacttgcgga gatatcttag ctgaaacata tggagagaac
    16021 gaaaattatc atactcatct ctatcaagaa aaatatcaac tattgaaaca aaacctattt
    16081 gactactttt gggatgaaga acttggcgct tttatcgatt gctatgaatc tgggagaaga
    16141 aatgtgacgc gtcatgcgaa tattttcgct gttttattcg attttgctaa caaagaccaa
    16201 cagcaatcga tcttaaagaa tgtcttattg aatgacacta tcacacagat tactactcca
    16261 tatttcaaat tctatgaaca agatgcttta tgcaaactcg gacagacgaa tatcgtctac
    16321 caaacgattc ttgattattg gggagggatg ttatctcatg atgcggtaac tttctgggaa
    16381 gaatacgacc ctactcaaac aggaaatgaa aagtacagta tgtatggaga tccttatgga
    16441 aaaagcttat gccatgcatg gggagcaagc ccaatctatc ttttaggacg ctattttatc
    16501 ggtttgtatc caacaaagcc aggttatgaa gaatttatga tacaacctaa tctagattca
    16561 tttgaaacac tgaattgtac gctacctatc aaagatggaa agatttcgct tgaaaaaaca
    16621 aaagataaga tcgtgattac ttctgataga acaggcggaa cagtgaaaat gaatggtaaa
    16681 agttatcctc ttctagcaaa acagccactt gtcttaacaa gctattcaaa aataaatgca
    16741 taaaacaaaa aactgcttgc gacatatcag aagactttaa atcttcttct gatattgtcc
    16801 aagcaatttt tattttgaag tttgcttaat tctgttcttt agaaacaatg tttgttcttt
    16861 ttccggcaaa aagaaagcta gataatacga caagtaaaaa gaaagcgatg attccaactg
    16921 tcaaggctat ttgaccgtat tctcctagtt tcccaagtac aagtcctggt actagataat
    16981 cagtatcggc aaatgtagaa gaagcgattt taagatctcc taatactgga aggataaata
    17041 atggcagcca actaatcaat aagctattga aagcagaacc taataccgcc cctcttctac
    17101 cgccagtcga gttgccgaag acaccagaag cagctccgca gaagaaatgt cctactaccc
    17161 caggaatgat aactgtagtt tgcaagccta tcatagcaaa catggatagt gttcctacaa
    17221 agaagctaac aaaaaagcca attaatacag cattaggtgc atatggaaaa acgataggaa
    17281 catctaatgc tggtttagaa tcgggtacga gttttttagc aatcccttgg aatgctggaa
    17341 cgatttctgc caacaccatt cgaacccctt gaaggacgat aacaaaccca gctgtgaatg
    17401 ttccagcttg gatcagtgca aagattacag cgttgtctcc attgcttagt tctctttcaa
    17461 caaatccagg accagctaac aaagcaagtg cgacatatac gatcgacatc aagagcatga
    17521 tcgatacagt agaatctcgc aaaaagctga aacttttagg aatctttatg tcctctgtag
    17581 aacggtcttt gtttccaaaa aactttccga tccatccact agaagcataa ccgatatttc
    17641 cggtatgccc taacgcaact tgattgtttc cagtgatgtt cttcataaac ggttgacaaa
    17701 gagcaggcgt aatagttaat agtgttcctt cgaagacccc gcctaataag atcatccaca
    17761 aatttgcagt aaaccccgcg cttactaaaa tgactgctgt tagacaagaa acataaagca
    17821 ttgcttgacc tgtaagaaaa atgaatttat atcttgtgaa tctagcaagt aaaatattca
    17881 cgatcatccc tactagcatg atcaaagcag taggtgtacc gtattgttgc aaagcaatcg
    17941 ccactactgc ttcattgcta ggaactactc cttgagtatg cagagctgtt tggaacatcg
    18001 aagcaaaagg ggttagtgaa ttactgatta aaccagcacc gccgatcagt actaggaatc
    18061 cgacaaatgt tttgatagtt ccttggatca cttctgaaaa tttcttcttc tgaagaacga
    18121 gaccaagcag tgcaatcaaa gaaacgagga tggagggctg actgacgatt gacacaaaca
    18181 aattcaacaa tttatccatt gatagttcct cccttgaatc atttttattc ttttgacaag
    18241 atcttcgtta ctttttcttt tacttcttcg ctgtcaatga tcgattgcaa aggtacgagc
    18301 ttttcatcag gtatagaagt tagcgtatct ttcaaagaat gctcaatgaa gaaataatca
    18361 gccatatcgc tagatacact tccaacatcc gtatgactta cttcaaactc gtccacatct
    18421 gctccaagat ctgcaagtgc ttgtttgatg ttcatctcta ccataaaact tgttcctaat
    18481 ccactttgac atactgcaaa aattttcata tcattcgtct ccttttctta tgatcgctaa
    18541 tatttcttct tttgttgatg cgtgtaataa atcttgaaga ttttgtttat tagaaagtag
    18601 ctttgttaaa tttgccaaag cccgaagatg tgcctcatta tcgatagcag ctaaacagat
    18661 gaacaaacta atgggatggg ctggattatc aagcagatcc acaggttgtt gaagtttcag
    18721 caaagacatc cctacttcgt ttacgccatc ttctggacga gcatgaggta atgcgatgtg
    18781 atcgccaata tggataaacg caccatactg ctccacgcgc tcgatcatag ctttgatata
    18841 actttcttct attttatttt ctgataatag cggctgtgcg gcaaatgtga ttgcttcttg
    18901 ccagtttttt ggttgatccg ataattggat catttcttct gttaataatt cagatagcat
    18961 aggacgttga tcctccttct tttctaaaag tttgttcatt ttacgattta atacttgata
    19021 gagcttttct tcgtctacgc cttcttttaa ttcgatataa ggcagtagcg ccgacaacaa
    19081 ttcttgggca gaaggcattg aaaatcctgg tatcagccaa tctctttgta cttgattgat
    19141 cagctgattt ttttccaagt cagtcatgat tggatgcaga acatagagag actttttctt
    19201 atcagtaagt gtgagaggta cagtagaaaa aataatatcg taagaagttt cgtcaacctc
    19261 gtgtaagcgg tcagctgaat tagtctctgt aaaaaagatc atcgggaata attgctgaag
    19321 ttcacttttt aagatcaatg aagagctaat gccacttgga cagacgatca cggctcttat
    19381 ttttctattt ctttcttctg cgtccgcttt tctgatctct ccgccaaata gtatagtgaa
    19441 aaaaccaatc tcttcttcag ggatgctctt ccctattcgt ctttctaacg gaagtaatgc
    19501 ttttcttgtc atttcgtaga tggaagcata ttgatgctta atttgatcga tcatcacatt
    19561 aggtaaataa aaagaatagg aaatacgaaa ataggcaggg accaagtgat aaaagacatt
    19621 catcaataat tcacgaaacg aagcaaattc tacggccatc aggaattcca tgcgatggat
    19681 gacttctgaa gaacattcca gcaaaaaatc aagtgcaggc tcctggacat ctccttcaac
    19741 gatcgatgct aagcaaagag agaaaaatat aatctccgaa ttttgatgtt cggaatagcc
    19801 aaggccagtc aatacttgtc gactaatgtc agtgactgca agagaagcca acagttcagc
    19861 atcttttacc agttccatgg atgtttttcc aattcgaatg gttgtcaaac tcaaataata
    19921 agctaattca tccattcgat ttggtgcgaa tcgaagatct gatagtgaaa tcgttttatc
    19981 tagcaaatct ctaactttgg cgtaataatc aatggaaagg gttgcggtta aatacgtgag
    20041 cccaaatctt ccagcaagtg actgcatcaa acgagcagtg cgattttttg cttctcttct
    20101 taatattgct tctgacccta gcagcctaaa accagttttc ctagtatacg acagctgaca
    20161 atcttccact tctaattcat ttcttatctt tttgatatct gacaggactg tccctttaga
    20221 aacctgaaga aaatcttgaa aatgaaaaac agaaacagac gttgcagtcg aataaaccat
    20281 caaataaaga agatctcttc gctcttgttc ggtatacaca gcttcaaatc gagtaatatc
    20341 caaaaatttt tctgcccagt cgattgctcg gaatttcttt atacggatac gggtatcatc
    20401 cacatattcg gcaaaaggag ttaattgttt gatcgcctcg cttgtattta cttcgatgat
    20461 ctctgctgct ttttctagac tgatttctct tggtgttttc aggagataac taattttgta
    20521 gttcacagcg ctgttcacct cctacttata ttctagtatg gtagcgcttt taaatcgagg
    20581 ggtgtttggt acaacttaca gttggatttt ttttacgact gtaccgaacg atttctatcg
    20641 aatggataca gaaaaaaagc aggtgaccaa atgtctcctg cttttatgaa ttgatttcgt
    20701 ccgatcaatg atgatcatgt ttattttttg aggtcagttc ttcaaatttt tctttgacat
    20761 cttcaaccgc tttttcgata tctttttcaa agtgattggg ctcattaggc tttgtttcct
    20821 cttctacaga atttgaacca gataaagctg tttctttatt ttctgtttca ccagagtttg
    20881 tgccagctaa tgattcttct ttgtcttctg tttcaccaga attatttcct gctaaagcca
    20941 cttcttcttc tgttttaggg gccattgaac ctcttaatcg tttgtgtttt tcaccgtcat
    21001 aatttgtcat gttggttcct cctagtagtt ctatactttt tcatcgtaca ataagacagt
    21061 ttatgcgtca aaaaacaaac ccgattctta caaatatgtc atatttttcc ttctgattct
    21121 tgaatgcttt tgattccttc ttccaaaatt ttcatttgta agattgtttc ttcatctttg
    21181 atgacttttt ctgcgccatt aactaatgtc tcgtaaatcc cttcgtagac acggctgtaa
    21241 tcaccaacct cggaaattac tttttcttca tgataactcc cttgttcatc tatataggtc
    21301 aatacaccat aatgttcggg caaatcgata ccaaagtctt catgatcagg catgtaaaac
    21361 attttcagat gttcttcttg tcgatctttt gtttgtttca cgaacatccc tttttttccg
    21421 taaacgacga aacttggacg ttctttgatg cggaaataac tggattttat cgaaattttc
    21481 atatttccat agaaaaaatc cagatcgaag taatcattca ttcgtccttt ccctaaaagt
    21541 tgacgtacat catagctgat atcatctggt tcgccgaaat aagaaagaac ttgatctact
    21601 gtatgacaag catgtccata taaatagctg ttcgcagtag aaaattcatg cgtattttct
    21661 ggtacttccg gtctgaaata atcataatgc atttctactt ctaaaatatc ccctaatttt
    21721 ccgctttcga ttactttttg gactgtcaag aagtcagaat cgaaccgtct gttttggtaa
    21781 cattggataa atagaccttt ttcttttgct aatgcaaata actcttctgc ttcctttgac
    21841 gtttctgcaa aaggtttctc tactagtaca ttcttaccat gttcgagtgc catttttcca
    21901 tattcataat gaaattgact aggtgtagtg atgacaacta attggatctc aggatcatcc
    21961 caaagatcag acagctgatc agtatattcg atgtcttcga atgttttcca cactggttca
    22021 gatggggaaa aaattttttt gatattgatt ttgttcgtaa gttttcttga aaaaggcaaa
    22081 tgatagcgat ttgtactctt tccattgcca agataagcga ttgtaagcat gagtggtgcc
    22141 tccttttatt gatgttttca gtatatccta gaaaagcgca gaaagtattt ataatagcta
    22201 ttctggtaaa aaagttcaga aaaacacttg ttatcattcg gtttggtgaa attaatgttc
    22261 aaagtaagga taaaaatcaa aaagatagta gcggaaccaa gtgtttatca gtaagataga
    22321 atttggaggt cagatcatgc ttattgaaga aaaactaaca aaacaagagt tgtttaccac
    22381 gacagagaaa cggattgtcg attacatccg taggaatatc gaagctgcag tctacatgac
    22441 gattgaagaa ttagcaaaag ctacgtatac ttcccattca gctatcattc gtttatgcaa
    22501 aaaaatggga ttcagcggat ttaaagaatt ccgtttcgag ctggggcggg aagttcatca
    22561 attgattgct cagttcaatc aaacgattga tcctaatttt cctttttcaa gtgaagatag
    22621 cactcagcaa attgcaaaga aaatggcgga tttatcgatc cagtcaataa aaaaagcaca
    22681 attgcagatt gaacatgaag atttagatca aatcggtaaa gtattgaaca aggcacagcg
    22741 aatctttctt tttgcaaaag gtgattctca aatcacggca aggaaattcc agaataaaat
    22801 ggtcaagttg aataagtttt tgatcatggc tgaagaatac agtgattctt cttggaatgc
    22861 agccaatttg actagcgagg attgcgccat ttttattagc tacagcggtc gcatccatca
    22921 ttatgaacga ataatgacct atttgaagca tgtagaggcc cctaccttgc tgattaccgg
    22981 aaatcagcat tcagaaatgg ccaaacaggc tgatatgtgt cttgttatat cgcaggagga
    23041 atatgatttt gctaaagtag cgactttctc ctctcaaatt gccttcgatt acgtattaaa
    23101 tacgttattc tcagtgatat attctcaaaa ttttgaagag aatatccgca acttgaaaaa
    23161 caaacaagaa ttgatgcaaa aaggccttct taaagaatat cggaaaactt aagaaaagat
    23221 cctcatttag taataagaaa gatagtgttt gataggctct ctttgatatc atcataaaaa
    23281 caggctgaag ttttcttttt caaacctcag cctgtttgtt tacggaacga ttcgaataga
    23341 tatattatag aaatgaggaa tagctaactt ctgtttctta ttttgaacga ttgacgaaaa
    23401 agatatataa gaaggaggaa aatcagtata catgcagcta acaatccata caggaaaaat
    23461 cgtttgttct cggatacttt ctgtacttct ttttgaactt cttttgcatg ataaggaacc
    23521 cggtgccctc tgacaagtaa gcgatgactg ttgaccatat aaggtgtaca agttaggagt
    23581 gtcacgaaat cctgcccttt ttcgattctc agatcttttg tatcagtcgg ttctaccgtc
    23641 ttgatctgat cgacttgata agcgagtgtc cgtccattga tttcgatata gaaagaatca
    23701 cctaaagtca gtttcggtaa gtctgtgaac aactttgctt gaggcagtcc tcgatggctg
    23761 gaaataacgg catgtgtatt ttctcccccc gtaggataag acgttccttc caataatgag
    23821 gcaccttttt ctagcagcac agtattcgtt tcatcaaaaa ttggcaaatg gacattgatc
    23881 tcagggatcg tcaaaatacc gatcgtatgt ttctcaaaat aggttctatc ctttttcgca
    23941 ggttcttttt tcttagtaaa gggatctgca cctggatttc caccttcttt tgctaattgc
    24001 tggtttttct ttgtcatgtt ttcttggatt ttagccatga cttcttcatt ttctttgacc
    24061 gcttgttgct gataatgaga gatgatctgc tgatccagat aattattcag cgcgtcgcta
    24121 acaaatggat aagctagtat ccctattcct gagagaagca agaaaagcat cagcagatct
    24181 agaagtttcc ttttctttgg tttcatttgt gttctccttg tgatgactgt ttgataataa
    24241 aagaaaaagg agcatttgcg taatccgctg ctcctttttc caattcttgc aaaaagacta
    24301 aatctgactg tgcttgcgtc tagcaaagta cagtccagca atcagtagaa ggactactcc
    24361 tgcaccgata tacacataga ttcccttacc gcctgttgaa ggaagtgtac ctttgtgttt
    24421 atttggtatt ttttcaggag aaatcaactg tcctgctgtt acataagatt gttcatcgat
    24481 agtaaatgct acacggtttg ttaatggaac atatttttct ggcgctttcg tttcttccag
    24541 atagtacgtg ccatatttta gacctgtcac atcgattaaa ccgttacttg tggttgtaaa
    24601 aaccgttgct gattctttcg ccgataccca gctgacggct tttgtggatg ggtcgatcga
    24661 taaatatttc gctgtgtcac tatcttgatc acgaacgacg aattctgctc cagcaagtgt
    24721 ttggtctgat gtaacgtcac catctacttt aacaaatcgt ttgcccccag taacgacatc
    24781 gactgacggt ggtgtttgat catttgtatg cccgttatcg acatttgctt ggttagaaaa
    24841 tcctttggtt ggatctgctt tttcgttcaa atgcatatag taaacgaatt tcaatgtacc
    24901 gccaggagtt aatgaaggaa tataattcgg atcaactgaa accgtgaatc cgtctgtttg
    24961 ctcagtgaca gaataattta ctgggtcgat ttctttattt ccatcatata aggcataagc
    25021 atacgttcct gaagaatcat tatcaaatgt taaagcagcg tcatgagtat cgataagttt
    25081 gaatgttgtg tacttgtttt gcgttccttc tttatcagcg atacctaatg ggatattgac
    25141 agaaatctca tattgaattt tttcaccgat tgcgacatct tttccattca attgaggtgt
    25201 tgttttatca actttagacg tatcattttt gatggtcttt tctactggtg tttggctatt
    25261 tgccaggatc tcaaaaggcg tgattgtttg cttttcaatc atttccgcat tatcaggagc
    25321 ttttacttct tctaaattat attttccaac ttctaaatga ttaacgatca actggccttt
    25381 ttcaatagat gcctcggcaa agtcattgtt gccgatgtca taagaatgac cagtaatgaa
    25441 atgtttggct ttggtttgat cagttgtcca agtgtacaat ccatctgtga cactttggat
    25501 gtattttttg acgcttggtg ttccttcttc tttagaaata ataaattctg ctccatttag
    25561 tgcttcgttt tcggcagtac cgatttttgt aactttcaac gttccatcat taccgactgt
    25621 atttttaggg tagagatgga tagtatctag ttcttctgtc ccgtatttat aagagccatc
    25681 tgcttgtttg atcatctcat atacagggaa agctaaaacc atgtttgcgg cagctgacac
    25741 tccgtctttt ggttcttctt tgatcgtgta gactgcatct ttcccatttt gttttttagg
    25801 taaagataaa gtgacatttc catcagcatc tgtcgttcct gaagcaactg gtgtaccagg
    25861 agtcaaagac tggactgctt gttttgctgc atccacggac gctcctttat ctcgttgcgc
    25921 ataaaattct tgagtgacgt tataaactga aaatgaaata tcggctaatc cttggtattg
    25981 atcgaattcg ctcatttctt tcccgctgtt ttggattaaa ggatcgggta aatcagtcat
    26041 tttcttttta tgaaggatga cttgagctgc tgtctcctct gcaaacactt tttttgcgcc
    26101 gaagctgttt gtgagtaatg gtaaaataag gaaaaggact cctaacataa cgtttatttt
    26161 tttatgattt ttcatttttt cactcctgtt ctttttttga taaacaacca gattgcaaag
    26221 cttgcgaata ccataccaac aacgcctaaa agtgtatttt tcgtttcgtt tgtttttgga
    26281 agaaatggac ggctattttc ttttttattg actactttca atatactgtc cgcctcttta
    26341 tcctcagaaa gaaaagttga aatggtaaac ggaatttctg tttcacttaa aatataacca
    26401 gaaggtgctt cgatctcttt taatcgataa cttcctgttt tcaacccgga aattgaaaaa
    26461 gcgccatttt tatcggaaat caggtgaagc gcatctgatt cgttctttgt ccacttgtat
    26521 ccgttggctg tttgctggag gtaatttcct gctttgtttt taataaggaa tactgcgcca
    26581 gctaatcgtt tatctggttt atttgtctcg attttgacaa attgcttact ccctgtatat
    26641 acagagactt tctttgtact gacaccaaaa ccaaaatcca gtgttccgtt gttgttgatt
    26701 tccttgttcg cctgtgctgt actgcttagc tgcatctgat aagaaatggt cagttttttt
    26761 ccagcatatt tttccaacga agggatcgaa aataaaacat caaatccgtg aggtttcttg
    26821 tgtatgacaa agccttctgt cagcttttct ccattcgatg aaatcgttaa actattaggt
    26881 aaaaacgtta atgcttcatc cgcactatct gacaattcga acttttgata gtcaaggata
    26941 tttacaggaa ttgtcgtatg caaggaataa gtgatttttt caccaatcgt aaaatcattt
    27001 ctaggctctt ctaatacctt ctcaaaaggt ggatcagggt agtcgttttc ttcattttta
    27061 ggatacaaat ggatagttga aagtttttga ttgttttgtc catgtacagg aagaacaact
    27121 accatattct ctgccttttc tttgacgact tctggtactt tggattcaat gaaaagatac
    27181 gctttatctc ttttttcttg atctttagcg gccagagaaa aagaagcgat accatcttcg
    27241 ttgttaagag ttgtagttgt tgcttctgcg gtaaacatac cggaagacgc accgtttttt
    27301 gcgatctctg cttgtgcttc ttctaccgtt ttgccctttt cccgtagatg gtaaaaagaa
    27361 tctgtgacat cataaacttg gaatgtgaca ccatttaatc ctcgatacgt ttgtaataaa
    27421 gctttttctt gtccgtcatt tggatgattt ttcggcagtt gcccgtttgg gaaaagcaat
    27481 ttgtgtaaag taatctgaac catttctgtt tctgtttctg ttgctacctg agtaaaagct
    27541 ggtttaaata gtaacaagaa gagacacata ctaagccaac caagtttttt catatttagc
    27601 tgccccctct ttcttttttc aaaaaaagat accaaataga aaacgcacaa ccaatcatcc
    27661 ctactagata gattcctaaa cggccaattc ctcccgtttc aggtaatggt acttttgcct
    27721 gattcgtgat gtctaaagaa atctggttgt ttttggctcc tggtgacaga acagattcat
    27781 gatcttgtcc atccacttga attgacccat cttcgtgtat aactatagta actggctctt
    27841 ttagaccttg gtatccttct ggtgtaaaag tttcggtcag cgtataagtt ccaggtttta
    27901 aattttcgaa tagaaaggta tctgtttctt gtccatcttt tggcgattct aagtccattt
    27961 ctggtccctg cagtctgaat tttgctcctt ttaatgtctg tccgttgtct tcttttttgt
    28021 gtaccgttag atcaaacgct tttagtcgat tttgatgagt gagcgtccat acattatttg
    28081 tcatctcggt attttttccc gtgtaaatga tttttccata tttatcgatt tggaattcat
    28141 aatttcccgg cgaagtatca tatccggcag gaccagctgt ttcaacgact cgatatttac
    28201 caggtgtgag tgaatcaaaa ctggcaagac ctttttcatt cgttgtttgg ctgtcaattg
    28261 gctgataagt accatttgct tcttttttct gtagggaaaa tgctgctcct ttaagattta
    28321 tctctttgtc cgtcccttgc atcgcatact tacgtactgc tacaggaact tcaacaaaag
    28381 gattttcaat agtcaactgg atcgtatcat cggaagtagt gactgttttt ccatcaatgg
    28441 ttaccgtacc atcagaagcg atcttgattt cccaagtagt ctttttgctt aatccatgcc
    28501 cttctggagc ttttgtttct gtcagcgtat aggtcatttc tttttgcaat ttgacatttt
    28561 ctggaagaga ataggttccg tcgccatgat ctgttagtaa agtatcaata gaatctcctg
    28621 ttaatttgaa aacagcgcca ataagatcct tttcacctgt agaggaattt ttcgttattt
    28681 tcaagtttaa cggtgtgaat tgtttagtat ttttccatgt cattgcatct atttgttgag
    28741 aatcgtatcc tggtacaggt aattctttga ttgtttggta actgaatgct tgaccttgat
    28801 tattgtattg aggtagtgat aatatctctt gataactttc tccgctattt gcagataatt
    28861 tgtcaatgtc tgcacgttcc cacgtatttg ttgtatcttt agctggttta atgattcgaa
    28921 tatatccgtt tttccaagct gcagcatttg tcgtatgttc ccgttgaatc tcaaaagtaa
    28981 cttgatctgg acgatcagct agattgttgt caaattcttc ccataacttt ttgatgtgaa
    29041 gactgactcc gggagcttta gcagatggta taccgaattc agctaattca ttggtatcaa
    29101 tgtttggctg gaaagttgtc ctgccgttca tttgatacca gaaatttgga tggaagtcct
    29161 cattttctgt ttggattctc acttggtaat ggatttggat ttcttggtct tttcctaaat
    29221 agatctgatt gctcttgatg gtatttcctt ctatggaaat agatggagtg accgttgtag
    29281 gacttgtccc cacactcttg actgaaagtg taccaggctg ataaatgaat ggttctgcga
    29341 ttggatcatt tatttgtcca ttgcttacag ttgctgagat ctgtacagct tttttggcta
    29401 ggtattcaga gatatctgtt gcatgatcag ctgattcata gtaaagatcg cctttttcat
    29461 ctgatgaaac catttgtcgc atacgagact ctacttctgc ttttgagaga ccagctgccg
    29521 gatcgctttg aagttggatg ccaagaccat gtatttcgat tcctcgttct ttgagtgcca
    29581 tcgcttctcc gatggttgcg ataaacgtac tgtcgattct tctggataga ttgttttggt
    29641 caggtgcatc atagattctt gagattagag aagtatttcc cggccgatct tgcgtattag
    29701 aaaactgagt tccgtaataa ttgctgcttg attgtgcgtg tacccgctgt actttatagg
    29761 aaaatgttgg tacaccatcc gtcagcaaaa cgatcacttt tttatgtcca tttggaacgg
    29821 atagcatgct tcctgcatct cttagtgctt tttgtgtaaa agtaccacca tttgtccgtg
    29881 aaggtgtaat ggattttact tgatttttca ctgaatcaaa tgaacccatc tgtactgcac
    29941 cgttactgta gctatatcct tcgcttgagt agccgacata tcccatattg attttgtctg
    30001 tgataccgct atctgctaaa gtatcgacaa aacgatcgac accaatcttt acttcaccga
    30061 tccgattatt gtcgttcata cttcctgacc agtctacgac caatacgaga tcaagaggcg
    30121 tgatatcttt ttgtacattt ccacgagcat tcaaataaac atcaaacaat ccgggtgtac
    30181 ttgtttcctt tgcatacttt cgtatcgcat agtctgcttg attaccggtt cctccatatt
    30241 cgatatacga atgggtccga tcattaggat ctccattcca agaatttata ccatcccatt
    30301 gatttgttcc gtttttattg ccttgatgat caagaacatt tgtatttcct gtaggttgcc
    30361 aactggcttt aggataagtg ccctgatcat ctgttgtata ttctgtagga atcgtttgat
    30421 tagatggcac actcacagct ggcaaggata atgtaggcaa tgtaaatggg ctgacagaat
    30481 tttcgttttc ttttgatgat tcttcttcta tttcgtctgg ctttggtact gacacatctg
    30541 tatctttctt attttctgtc tcatcaataa tgtcatattc tttattatcg acgatcagct
    30601 tgtttgcctg gctgtggtaa agtgctggat cgattggaaa actcagatcg atagtttgtt
    30661 tagtgtttga tgagatttcc aacgtaattt tcccgctatt tacctgataa gcatagccat
    30721 ctccttcttt tctttccaca tcacttaagt aattttcgac tagaccaatt tctttttcta
    30781 tcttttcagc actggtgtta ttcgcattta gcttgatgtt cagtgctttt tttccagact
    30841 gatccgaaat gacttcagct gaaattgttt ctaaaactgt tggatcatct gtagtgtctg
    30901 ctattgcaga taatggcaca aagtttgata aactcaatat tatcagcatg aaaataactt
    30961 tcagtttctt ccctgtggtc gtcatttttc ccctcctttt tctttttttc tgttttgacg
    31021 tgaatagtga aaattattaa aataaacagg cgttacataa ttttatttat tttaatatga
    31081 taattattat acgaagggcg ctaaagggaa tcacaccaat tttcatacca attggtttca
    31141 tttatgaagt tgattccttc gattaatgag cgtttcattg aaagaaaagg gttgagggag
    31201 aaaaacatat gatcaggttg ttggaaggaa actatagaaa gatggaaaaa atcattcgaa
    31261 ttctgtccat ttctgacaag tggatgacta aaaaagaact tgccaaacga attggtagtt
    31321 cagaatctac atttgtaagg tatatcgaag agatcaaaat ccgctgggga acggatgtaa
    31381 cgattcaatc ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta ccagcttatt
    31441 taaatgttct gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta aatgagatta
    31501 ttgtcaatcc gggtaaaacg attgagtatt attgtgaaaa aatttccatc agccggtctt
    31561 cttttacacg taagttgaaa caatgcaatc aaattttaga aagctattta ttaaaagtga
    31621 tcgttgacca aggttttcag ttaactagcc ttaaaagtga gctccagttg cgtatctttg
    31681 ttaccttttt ctttttaagt tactacggaa gacgcacgtt gccatttcca atcgaaaaaa
    31741 agaagatcaa agaaattatg gtcaagaata attgtcagat gaatttgttt caggaagaaa
    31801 gcccatatga gcaatctttc tttattatgt attttgttgt gcatctcatg agagaatccc
    31861 aaggaactcc atttgaatgg aaagctggag actcttatgt acagttgata aatatcgata
    31921 aaaaagactt cttaatcttg aaaaatctct taggaaaatt gaagtacagc agctatataa
    31981 aagtagtgaa ctattttgtc aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg
    32041 aaatcagtga gcagatacga aaaaatatgt cctactgttc gttttcatcc gctgattgca
    32101 aagaaaaaaa cgaagaatat gttgttcaat tattaacgca aatttatttt ttctcccgta
    32161 ttttcccata cgatacgact catttgacga tccgtgcaac ttactttgca cagcagatgc
    32221 aacaccgcca gatacgtcta tatgctggat ttcgtgcaga acttcaattt tattccactc
    32281 tactgaatca gaatctgatt tattatttat ctagctatat cttttggatg cttatcggta
    32341 atcctgaaat tcaccattat acttctgata aaaaaatact gattatcagc gacttaagtc
    32401 ttcgacatag tcaatatatt gaagaatata tcagtgatat tcttgctgtg cataaaattc
    32461 actctgagac aactgctatt actgaagatc agctgtcgaa atacaatctt ctcgagtatg
    32521 atctgatcgt tacgaatcaa cccattttga atgcccatgt tcctcatata ttgattgatg
    32581 atagtgtgtc attcgcaaat gaagaagaac tcatccgctt attcgagtta tgaataatca
    32641 aaaaacagct atgtataaac caccctgata aaaatttcag gacaaataca catagctgtt
    32701 tctttttatt gtattccttc agcaatccaa cgagatttat agcgaaagtc ttttaacaac
    32761 tcttcttttg tccaattatt tgcaatgaga tccgtcaaag tgttcattac gagggtcatg
    32821 gaaaagtata ggaaactaac tgcttcttcc gacgattgaa tggagaaatg attgattctt
    32881 aataattcca gccatttttc gttcattgga gaatcagccg atagttctct acgtttggtt
    32941 gtctgctgat catttcgctg ggttaacaag tataaccttt gccaatagat actttcgtga
    33001 tctaagtcgc agcaccactc taaatatttt tcgatcccca aaaagaaatg ttgtttgttt
    33061 tcgctaatga attttaagat atcttgatgg actatgtgag aactgctttt gatcgtatac
    33121 aaataagcat cttccaaatc ttgaaaatat ttataaaaag caccgcgcga catccccatc
    33181 ttgtcaacga tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt
    33241 aataggtcat cgattctctg tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc
    33301 atccacatct ctcctttatc tctgattata cgtggatagc ccttcattgt catgaaggat
    33361 tcattccttc tttttctctt cttctgtcaa gtacccatct tccattcgat aaacgcgatc
    33421 actccattga atcatacgga catcatgagt aaccataata gtcgcttttt gtttctcatg
    33481 agcttcttta actagtaatt tgacgacttc atatgcatga tctgtatcca aactagctgt
    33541 tggttcgtct gctaagatca agcttggctc attatataaa gcgcgagcaa ttgccactct
    33601 ctgtctttct ccaccagaaa ggtctttagg ataactattt ttaagtgact ggatatctaa
    33661 agattgcagc aaggaatcga tttttgttga atcttctttt tttgaagtaa cttggtcaac
    33721 tagttcaaat tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa
    33781 tcctatttct ttaaagcgta aatctgctcg ttttttttct ggaagctcag ttagtgagtg
    33841 accgttgatc actatttctc cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa
    33901 agtactttta cctgaaccag aaggaccaat gatgctgacg aattctcctt cctttacata
    33961 aaaatcaatt gctttcaaag ctgagacctt tgtatgacca gaaccaaatg ttttggtaac
    34021 ttttttcatc gttaatattt ttttcatttt tattaacctc ctattgctgt aattgggtct
    34081 actttggtta tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc
    34141 ccactatata gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc
    34201 agactagtca aataagcaaa caaaagagca agcagaacgc ctaccacccc aacaataaag
    34261 gactgtgcta ctaaagattt agcgatgaag aaatttttga tcccttgtgc tttcattact
    34321 ccaaaaatag cagtcttctg aagtgtcatc acgtacatga aaataccgac cacggcagca
    34381 accactaaga acaaaaagta tatcatcgca tttaaggtca tattttgggc agaatagcca
    34441 ggaatacttt caatgaatgt cccaatagtt aataactgta gacccttcga tgctttttct
    34501 ttcgaaattt tagctgaatc ctttattacg atcccattga tcggctgttc ttcttcagaa
    34561 gcaaacggct gttgtccata cttcaaagca gtccacgtat ctagggacgc atagatgacg
    34621 ggagaaactg tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa
    34681 ctgccaattt tgatcgtatc tccaatttta tagcctgaat cagccagatt ttgagagatg
    34741 atgatttcat tctttgtttt aaattcatgc cctttcgtga gttttggtaa taaaaatgct
    34801 tctttcgttg tgccgaaaac tgtaacattt tctttgtttt ttcctttgat tgctccgctg
    34861 tacaatccga ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc tcttgttaat
    34921 tgtgaagctg caaatacttg atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa
    34981 tcatcgattg cttttttaaa ctcttctgaa agtccattcg ctagtcctga caacatgaat
    35041 acgacatacg caatcaagaa cataataccg acgatcaagc catatcgtaa tttagaatag
    35101 cgaatttctt tgatagctag aaacatttgt ttttcctccc tatgatagat tgatagtgac
    35161 aaaatgtcac ttgttgaata gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt
    35221 tttcttatca ataaattgtc taagacatct ttcgataaaa ccttaacatg agtcataaaa
    35281 aatttattta agccttattt atgctttcca tgataaacta aagtgaccaa atcaatgagg
    35341 tgggaaaatg attaaactat tagtagtaga agacgataaa gtattatcca ataatataaa
    35401 agaaatactc gctgcaatcg gcgagataac acaagtttat gatggtagtg aaggactgtt
    35461 cgagttggaa agtggagttt atgatcttgc aatcttggat ttgatgatgc cggaaatgaa
    35521 tggatatgaa gtgttagcaa aaatacggaa agaaaaaata cagactccag tattgatttt
    35581 gactgcaaaa gatagtttgg aagataagat tgaaggattt gaacgaggtg cagatgatta
    35641 tctaacaaaa cctttctatc gagaagaact gatcatgcgc gtgaaagcat tattgaaacg
    35701 ctcgctaggt ctctttgatg aaaatgttct gaagtataag gatatcagtt gtgatttaaa
    35761 aagcaatgaa gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt
    35821 gctcgtttat tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg
    35881 gatctgggga tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt
    35941 acgaaaacat ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg
    36001 ttatatactg caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc
    36061 gcttttttgc gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta
    36121 ctttacagat attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa
    36181 attttgattc tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt
    36241 taaacccgac tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat
    36301 caaatgaccg ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata
    36361 aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc
    36421 tgaataccat ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc
    36481 gttcgattac gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag
    36541 aaaatacaaa tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta
    36601 tgattgtttt ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc
    36661 gccctattat tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat
    36721 tgcgcacacc gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc
    36781 atactattat tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca
    36841 ctggattgac ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt
    36901 caaagcaaat gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga
    36961 tggccatcat agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa
    37021 ttgatgaaaa aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata
    37081 cgaatccaga agataagatc attttggatt catctgttaa ccataaagaa tggctgattc
    37141 acgtaaagaa tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat
    37201 tttatcggga agatcaatct cgggcgaaag agactggcgg atacgggctt ggcttggcaa
    37261 ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc tgtatccaat tatcttccac
    37321 aaggagtcga ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa
    37381 aaaagggctg tgacaaaaat cttgtcacag cccttttttg ttgcgaataa acggtgttcc
    37441 agaagtagct tcttcggaaa taagccgaaa tttctttaag ctgaccactt ctgtcacaac
    37501 ctctttagct tacgcttacg attttttatt gcggtcttca atgtgtacat caccgcttgt
    37561 gtcaatatcc aattcttctc ggctaacatc ttctgtcact ttttcaacat cttcatgttc
    37621 ttctttatga atcccaactt cttcacgaat aactgtatgt ttgcttacat ctacttgctc
    37681 ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga
    37741 ttgatctgtt actggtttgc gttcgatgac gatttcttca cgtttgaccg gtacttctac
    37801 cgtttcagta tcgttgacca catgtttgtg gatgtcgact tctccagttg tcacatcatg
    37861 tgtatttaca tctaattttt cttcttttag ttgaattgta tcatcatcta gatttggttt
    37921 gcgatcgatg tcttcagtct tgcttgtacc cattgtatca gtctcgccag gtacgtccat
    37981 cggatcagtt cctggtgtca tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc
    38041 acctacgtcc attggatctg ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc
    38101 tgggctaaca gtatcttgtg tatccattgt atcttctgtt gtttctggct gacgatagcc
    38161 ttttactgtg atgacaaatt ttccatcaga aatatctcct ctatattgat ataaaggatc
    38221 attttctggt gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat cttttatttt
    38281 ttcccataaa gaacggtctt cttcaccatc catccgattt tcatctgttt caacatctat
    38341 accagacaat ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat aaataacgat
    38401 atcatctcgt tcataccctt cgttacgcaa ccgttcaatt actgataatg cttcttccct
    38461 cgtactatag cttccgatga ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa
    38521 atagatttat gattggtata
//
LOCUS       DAYYOA010000015        34956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000015 DAYYOA010000000
VERSION     DAYYOA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.015"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..636
                     /locus_tag="ACHBKA_000705"
     CDS             184..636
                     /locus_tag="ACHBKA_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444420.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(760..1611)
                     /locus_tag="ACHBKA_000706"
     CDS             complement(760..1611)
                     /locus_tag="ACHBKA_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9444421.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1624..2409)
                     /locus_tag="ACHBKA_000707"
     CDS             complement(1624..2409)
                     /locus_tag="ACHBKA_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9444422.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(2761..3702)
                     /gene="ribF"
                     /locus_tag="ACHBKA_000708"
     CDS             complement(2761..3702)
                     /gene="ribF"
                     /locus_tag="ACHBKA_000708"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM9444423.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(3706..4629)
                     /gene="truB"
                     /locus_tag="ACHBKA_000709"
     CDS             complement(3706..4629)
                     /gene="truB"
                     /locus_tag="ACHBKA_000709"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM9444424.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(4997..7359)
                     /locus_tag="ACHBKA_000710"
                     /pseudo
     CDS             complement(4997..7359)
                     /locus_tag="ACHBKA_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            7524..7980
                     /locus_tag="ACHBKA_000711"
                     /pseudo
     CDS             7524..7980
                     /locus_tag="ACHBKA_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(8137..8484)
                     /gene="rbfA"
                     /locus_tag="ACHBKA_000712"
     CDS             complement(8137..8484)
                     /gene="rbfA"
                     /locus_tag="ACHBKA_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM9444425.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(8511..10817)
                     /gene="infB"
                     /locus_tag="ACHBKA_000713"
     CDS             complement(8511..10817)
                     /gene="infB"
                     /locus_tag="ACHBKA_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM9444426.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(10830..11141)
                     /locus_tag="ACHBKA_000714"
     CDS             complement(10830..11141)
                     /locus_tag="ACHBKA_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM9444427.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(11138..11431)
                     /locus_tag="ACHBKA_000715"
     CDS             complement(11138..11431)
                     /locus_tag="ACHBKA_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM9444428.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(11453..12628)
                     /gene="nusA"
                     /locus_tag="ACHBKA_000716"
     CDS             complement(11453..12628)
                     /gene="nusA"
                     /locus_tag="ACHBKA_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM9444429.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(12651..13124)
                     /gene="rimP"
                     /locus_tag="ACHBKA_000717"
     CDS             complement(12651..13124)
                     /gene="rimP"
                     /locus_tag="ACHBKA_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM9444430.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(13269..17627)
                     /locus_tag="ACHBKA_000718"
     CDS             complement(13269..17627)
                     /locus_tag="ACHBKA_000718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM9444431.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(17839..19548)
                     /locus_tag="ACHBKA_000719"
     CDS             complement(17839..19548)
                     /locus_tag="ACHBKA_000719"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM9444432.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(19616..20884)
                     /gene="rseP"
                     /locus_tag="ACHBKA_000720"
     CDS             complement(19616..20884)
                     /gene="rseP"
                     /locus_tag="ACHBKA_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM9444433.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(21045..21845)
                     /locus_tag="ACHBKA_000721"
     CDS             complement(21045..21845)
                     /locus_tag="ACHBKA_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM9444434.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(21842..22654)
                     /locus_tag="ACHBKA_000722"
     CDS             complement(21842..22654)
                     /locus_tag="ACHBKA_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM9444435.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(22849..23406)
                     /gene="frr"
                     /locus_tag="ACHBKA_000723"
     CDS             complement(22849..23406)
                     /gene="frr"
                     /locus_tag="ACHBKA_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM9444436.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(23409..24131)
                     /gene="pyrH"
                     /locus_tag="ACHBKA_000724"
     CDS             complement(23409..24131)
                     /gene="pyrH"
                     /locus_tag="ACHBKA_000724"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM9444437.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(24267..25148)
                     /gene="tsf"
                     /locus_tag="ACHBKA_000725"
     CDS             complement(24267..25148)
                     /gene="tsf"
                     /locus_tag="ACHBKA_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM9444438.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(25247..26029)
                     /gene="rpsB"
                     /locus_tag="ACHBKA_000726"
     CDS             complement(25247..26029)
                     /gene="rpsB"
                     /locus_tag="ACHBKA_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM9444439.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            26388..26867
                     /locus_tag="ACHBKA_000727"
     CDS             26388..26867
                     /locus_tag="ACHBKA_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9444440.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            27084..28175
                     /gene="phnW"
                     /locus_tag="ACHBKA_000728"
     CDS             27084..28175
                     /gene="phnW"
                     /locus_tag="ACHBKA_000728"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM9444441.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            28168..28296
                     /locus_tag="ACHBKA_000729"
     CDS             28168..28296
                     /locus_tag="ACHBKA_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM9444442.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            28300..28845
                     /locus_tag="ACHBKA_000730"
     CDS             28300..28845
                     /locus_tag="ACHBKA_000730"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM9444443.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(29299..30990)
                     /gene="argS"
                     /locus_tag="ACHBKA_000731"
     CDS             complement(29299..30990)
                     /gene="argS"
                     /locus_tag="ACHBKA_000731"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM9444444.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(31410..32357)
                     /gene="arcC"
                     /locus_tag="ACHBKA_000732"
     CDS             complement(31410..32357)
                     /gene="arcC"
                     /locus_tag="ACHBKA_000732"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM9444445.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(32472..33491)
                     /gene="argF"
                     /locus_tag="ACHBKA_000733"
     CDS             complement(32472..33491)
                     /gene="argF"
                     /locus_tag="ACHBKA_000733"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM9444446.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(33582..34811)
                     /gene="arcA"
                     /locus_tag="ACHBKA_000734"
     CDS             complement(33582..34811)
                     /gene="arcA"
                     /locus_tag="ACHBKA_000734"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM9444447.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         9433 a         7494 c         6169 g        11860 t
ORIGIN      
        1 attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc tttagagaaa
       61 taaaagttag aattttctga cttgattttt acaatgacct taattaaaat cgttctttct
      121 acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat aggagggtga
      181 acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt tactacacgt
      241 tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg cgggcaatgg
      301 aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga ggaagcagca
      361 aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa ccttcaatat
      421 cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca taacgatgtt
      481 tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa tcatgcggag
      541 atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat caatgaaaaa
      601 ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg ctagaaattc
      661 cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt ggttcaaaca
      721 aatatttttt taataaatga aattttacca tctttatcct caaataaaga ccgtgaccat
      781 gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt ttggtgtctt
      841 attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta gctgtgtaat
      901 gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca atgctgacac
      961 actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa atgcggttgg
     1021 tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt gcggaaagaa
     1081 aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc aataaccaat
     1141 acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc caggatcatc
     1201 actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg ctccaatgat
     1261 tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg aggttccgac
     1321 taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt tgaaagatgt
     1381 atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa tcaaaaatgt
     1441 agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga caagcaccgc
     1501 tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt tcaaaatcag
     1561 tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca tttttattca
     1621 cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat ctgcaaatag
     1681 tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg tcgccgctat
     1741 ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt ctttttgttc
     1801 aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc ctatactatt
     1861 gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata attttacccc
     1921 gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat gaacagaaga
     1981 catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag ccgctttcgt
     2041 tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt caaggctaag
     2101 ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt ccagtgtccc
     2161 aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac tgacatctgc
     2221 ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt tttctgctcc
     2281 ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga atcgttgagc
     2341 aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt tttctaattc
     2401 cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga taacttttat
     2461 ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga tacgctctag
     2521 caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag gttgtgacag
     2581 aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct gaaaaaccat
     2641 ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct gggcttattt
     2701 atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat gcataatgtt
     2761 ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt gttcaatcaa
     2821 ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc gtaccgcgac
     2881 atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag tcagctcccg
     2941 tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc atgtttcccc
     3001 aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc tctttacttt
     3061 gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga caactacccc
     3121 ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc cgctgtctag
     3181 taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt tagaaactgt
     3241 gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg caatgtcttt
     3301 tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc ctacgatgta
     3361 ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat caacttcata
     3421 taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc tagtcaaata
     3481 tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat tgaaagtcat
     3541 caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta tgactttctg
     3601 gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt cttctggaat
     3661 ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt cctcatttct
     3721 caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat aaatactgac
     3781 aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga gcgcaaatac
     3841 agtataatca agtctcatac cgttttttac tttctgccat acttcatcag taatatctac
     3901 tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga ttgtcccatt
     3961 tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca taccggcact
     4021 tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc ctgtatctac
     4081 agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc aagaaatggt
     4141 tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa tctgagcctt
     4201 tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc gcttaccgtt
     4261 gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa atgaagccat
     4321 cacttcatct attttttttt cagataaaga catatctact ggttttgttt ctacccgttc
     4381 tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta catagatttt
     4441 cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa tgggcaacac
     4501 accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat gtaagatttt
     4561 tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata aaggtaataa
     4621 cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat aacacattgc
     4681 atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg caataaaaac
     4741 gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa aggagggaat
     4801 agaatggaag attttttctt tgcaccattt gattggattt atgggtggct tacaggtaaa
     4861 tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc cagtcttttt
     4921 tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc tattttgcgg
     4981 gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat acttctggca
     5041 gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga tccatttttt
     5101 tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt gccaaatgat
     5161 aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct ccattttctt
     5221 tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg cggaccaatg
     5281 tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat tgatcaaatt
     5341 cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat aaatctacga
     5401 cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt acagctgcta
     5461 atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt tcctcctctt
     5521 ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg gctgcttctt
     5581 ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg attttttctg
     5641 acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca ggcagatatt
     5701 cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga tcttggtggc
     5761 tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt agcgaaggat
     5821 aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc tgagtcaaca
     5881 tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc gtccagtcag
     5941 aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt cgatgacgcg
     6001 accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc attggcgatt
     6061 tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc caaatatacg
     6121 gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt cggtcatatc
     6181 ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc aatctggcat
     6241 aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca ttctttggtc
     6301 accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat caaataattt
     6361 ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca tatgttcttg
     6421 atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt ccccgtccca
     6481 ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc attttggcat
     6541 cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa ttttctcgat
     6601 tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat ttccttcaat
     6661 aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat ctgatttacg
     6721 tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg gtaaaacagc
     6781 tccatctatc actggattga taaagacatt tgagaaccag cttccagcta aaggaccggt
     6841 ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt ctaatacagc
     6901 tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag aacctggtgt
     6961 ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga ataatttttg
     7021 cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc catctaaata
     7081 agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa acatgttcaa
     7141 atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc caggaatcgt
     7201 tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac gtctcagtaa
     7261 ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac gccaccaagc
     7321 cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc cttttcatta
     7381 ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt ctgttcattt
     7441 gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat ttttcctttc
     7501 gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct gttccgcagt
     7561 tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt aacacattgc
     7621 tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt atgtaaaagt
     7681 ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa tttctacagg
     7741 gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa tatcaataac
     7801 ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt ttatggtgca
     7861 ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat agattacaat
     7921 ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat atactactag
     7981 aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc tgacacgccg
     8041 caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa aaaatcccgc
     8101 aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca agtcgcgaag
     8161 caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga acaattcagg
     8221 tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg atgctttatt
     8281 caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc tgtaatagat
     8341 cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt tctccacacg
     8401 agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt cttgaccgac
     8461 tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt ttagtcattt
     8521 ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc attgaatttt
     8581 tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc tttgaatcgt
     8641 ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac acggacaccg
     8701 ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc aactttagaa
     8761 actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa ttcagggtct
     8821 agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat acggtgaaga
     8881 cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg acgaacgttg
     8941 aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc attgattgct
     9001 ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt cttcaatgat
     9061 gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt aacttctttc
     9121 aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct tgtggcagca
     9181 cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc tgtcttttcg
     9241 tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc agtgatttct
     9301 actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt cattacacgg
     9361 acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt tccttcttgg
     9421 acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat cactgttccg
     9481 attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa caggatcatt
     9541 tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa gatcgtttct
     9601 ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac atgttgaggg
     9661 tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc tgcagctttg
     9721 gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc aacaactaag
     9781 atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc cgcatgtcct
     9841 ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc accgatatgc
     9901 tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt atccaataaa
     9961 gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg tgagactaat
    10021 ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat gtcaacttgg
    10081 actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt atctttatcc
    10141 agtgcttggt tttgattgac catgacacca agcataaata attttttaat gatttctgct
    10201 ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt atactctaat
    10261 acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga ttgttgctgt
    10321 ttgccttttt tacctttttt attataacga ttacggttat tattattaaa tttgccgcga
    10381 ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc ttggctcgtt
    10441 ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt tggttttttt
    10501 ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt atttttattt
    10561 ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg attggctggt
    10621 tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa cgcttgttgc
    10681 aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc gatgccaagt
    10741 tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc taattcatag
    10801 attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt ggtcttcttc
    10861 gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat ctgcgtgatt
    10921 tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt atcttttatc
    10981 ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc tttttgtttg
    11041 cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg catcgctaga
    11101 ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat aactctttac
    11161 gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct gttaatgttg
    11221 tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct tccggctcaa
    11281 gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat acttcccctt
    11341 cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct gaaaccacgg
    11401 atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa agttaagctt
    11461 ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca tcgtctgtta
    11521 aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt tgttcttgat
    11581 tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg ttagtcaatt
    11641 tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat tggtagtctg
    11701 gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc atgacttgtg
    11761 caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg acgatatcca
    11821 tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt ggcccgacac
    11881 atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt gaacgatcgc
    11941 cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct ggtacttctt
    12001 gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact tgcggacctt
    12061 tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt tgataaaatt
    12121 catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga ttgacataaa
    12181 tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca taagcactga
    12241 attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata acttgtttag
    12301 ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg atcgtatcac
    12361 cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc aactgtgaat
    12421 ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct tttttctgat
    12481 caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca gaaaccaatg
    12541 ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct tctaaagcgt
    12601 ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga ttaaaattgg
    12661 atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt ttctcgtgtt
    12721 tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa tccttcatat
    12781 tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc aagtgccttt
    12841 tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac ctccaggaaa
    12901 taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag tttctcactg
    12961 acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac tcgtaagaac
    13021 cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg ttcatctaaa
    13081 atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt cgcctcctta
    13141 aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc actcacagtt
    13201 agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc agacaggaaa
    13261 acagatgctt atagcatatc gaataacgat aattgatttt catctggtag atctttcaat
    13321 acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg ggtggcaaga
    13381 tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt tgccacgttg
    13441 gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc aataacaaag
    13501 ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg ttccagcatt
    13561 tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc atctaatcct
    13621 ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca catggagact
    13681 agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat agggaaataa
    13741 accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg ggctttcgga
    13801 aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt attttctttc
    13861 atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg tcctttacga
    13921 acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat caaataaacc
    13981 ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg tcgaatcaat
    14041 tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat ttgcagtagt
    14101 tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg agtgccgaat
    14161 tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac tccaagtact
    14221 tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt ttgtggatcg
    14281 atccctgata aatcttgaag catccgaata acagttgggt catcatgccc tagaatatct
    14341 aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt ccattccgaa
    14401 ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat ataatcggga
    14461 ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt agcgcctttt
    14521 gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc gtatcctttt
    14581 acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg gtaaacatag
    14641 tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc acctgagaag
    14701 ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc aaatggaata
    14761 tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc tggcatgtca
    14821 aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc tggacaataa
    14881 taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac aaagctagag
    14941 ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg cactagtttt
    15001 tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa ctcttttttt
    15061 aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc ttttgtgtaa
    15121 ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag ttcatctttg
    15181 actggaatca cttcttcgca gatatctgcg attttattcg tattttctac tacgatctct
    15241 ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt tgttcggaaa
    15301 tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga attgatcaaa
    15361 atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt tgcaacaacg
    15421 attttatcca aactttttcc gatttctacc aaattttgga taatttcttc caaatcatgt
    15481 tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg cataacttca
    15541 atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg catcatggct
    15601 tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg catctttttc
    15661 aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga cattgaaatc
    15721 agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac atggaaaggt
    15781 cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc atgataaagc
    15841 atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc agttgcttca
    15901 gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt agataacaca
    15961 cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa cgtatcaatg
    16021 actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa gaaccccata
    16081 tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag gaacatccgc
    16141 aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc agtcagatct
    16201 accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct ttcgatcaca
    16261 tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac tgcagacaat
    16321 cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc atgtgcatca
    16381 ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta taagattttc
    16441 acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc accgtggtct
    16501 gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc agagatgctg
    16561 tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg tttttcccct
    16621 tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg cgcattcatg
    16681 accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa ccagctaccg
    16741 acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt tttgacgata
    16801 aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga acgtagttct
    16861 ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc ttcaacgata
    16921 ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg gatgtggatg
    16981 ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc gtggacaact
    17041 agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg ttcttcaaaa
    17101 agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg aaattttggg
    17161 aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt caaataagaa
    17221 atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac aggtacctgc
    17281 gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa agcttgggcc
    17341 cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat attcagtttg
    17401 acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg atatagcata
    17461 ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt acgatggacc
    17521 actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc ttcctctagt
    17581 tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga tatacgccct
    17641 ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca gtacctttta
    17701 gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc aaatcgcagt
    17761 tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct atttttattt
    17821 tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag tattcaccaa
    17881 ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt ctacgattcc
    17941 atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat cagaatccgc
    18001 gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc cagcttttgt
    18061 catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta gattcatttg
    18121 gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt gttcatctga
    18181 atgttgttct acgattgctg ataataaacg acttacaccg ataccataac agcccataat
    18241 cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt cactgtaacg
    18301 agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa gaacaccatt
    18361 gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag aaagtacagt
    18421 gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg ctccagtaat
    18481 ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat ctgaaacacc
    18541 gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt cttctgttgc
    18601 ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga cttcatggtc
    18661 tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag acttgatgat
    18721 tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta cgttaggtgt
    18781 agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat agtagcttgt
    18841 agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg tatcctcccc
    18901 gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc caccgtctcc
    18961 gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg ctttttcgta
    19021 atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg catctttcat
    19081 gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac gatatttagg
    19141 ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat tacgcatcaa
    19201 atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat tgcggtcttt
    19261 gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc aaagttctgc
    19321 tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt cttcccgcat
    19381 aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat aaatacccgc
    19441 tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata cttctgcatc
    19501 atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc tatatactcc
    19561 tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac tttttctaga
    19621 aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt aaaaatccaa
    19681 agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg cgaatccctt
    19741 caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc agattgacga
    19801 ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca ccttgatttg
    19861 ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct aaactgaaac
    19921 ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa gcttgacgcg
    19981 tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata ccaagttggc
    20041 caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat tgcacctgac
    20101 cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg actgttgtca
    20161 gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca ttttctttta
    20221 agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta gtgttcgtca
    20281 cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc aaaataaagt
    20341 tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc gattggaact
    20401 gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc gtcgcatcgt
    20461 gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca gtgatagtca
    20521 gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc aattggattt
    20581 tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga gaaagaggca
    20641 ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca taaccgccaa
    20701 taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca taaattttcg
    20761 gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca aagaagaaat
    20821 gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc agaatcgttt
    20881 tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac gataaaacac
    20941 tataatttta ccatagtata ccaaagacac caatagtatc aatataatga aaaataaaaa
    21001 cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat aatgccaaat
    21061 aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat tccaccatgg
    21121 cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc agattcgacc
    21181 aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat gacaaacata
    21241 ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc gactacaaca
    21301 gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat atcaggactt
    21361 aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt tgcccaaaca
    21421 ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac aaaattctgg
    21481 aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc aatcgtatac
    21541 gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata aaataacata
    21601 aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa aacaagtaac
    21661 actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc tttcatccga
    21721 aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac cgctatgcct
    21781 ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac cacacgttgt
    21841 ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat cgacggtctc
    21901 gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca ggccaaagcg
    21961 cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta ctaattcgct
    22021 cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc aagaaacctg
    22081 tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt tctccttttg
    22141 ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt ccataattca
    22201 aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca cgtcttacag
    22261 catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact ttgacatttt
    22321 ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc atgagaaaac
    22381 ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc gtcaacactt
    22441 tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg ccttctttat
    22501 ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg tccatgatga
    22561 tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt ttttcttgaa
    22621 tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata cttaaaagat
    22681 tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa tcattgtttt
    22741 tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag aaatataaat
    22801 aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt aaacttccaa
    22861 taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt tatctgtcaa
    22921 tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac catttttttg
    22981 ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga tttttgcatt
    23041 ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg tcagttgcgg
    23101 gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat cacttgccat
    23161 aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca ttaaaacacg
    23221 tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac cgtagtagtt
    23281 cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt gcccaagttc
    23341 acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca taattgcatc
    23401 tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat tgcacggcga
    23461 atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc catgcttagt
    23521 gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag atgagtcagt
    23581 tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac accgtctaca
    23641 ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa tgctgctgtt
    23701 gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat ccgtcctttt
    23761 tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg catttcaatc
    23821 gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa agctaaggca
    23881 ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat gcccatttgt
    23941 gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat ttcaacgccc
    24001 agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg tttgattcca
    24061 aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg ctgatatttc
    24121 ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata cttagatgat
    24181 ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg cacaaaccgt
    24241 gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac ttcttctaca
    24301 aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa tgttttaaca
    24361 gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt aacgaatggt
    24421 tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac cattttttca
    24481 acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt tttttcatgt
    24541 tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat agctgctacg
    24601 tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac agtcaatacg
    24661 gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc tttttctaca
    24721 acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc gatcaagtca
    24781 gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc tgctggtttg
    24841 ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc gtttttagaa
    24901 acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt cacagcaact
    24961 gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc cattcctttt
    25021 tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa tgcttttttc
    25081 gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt agctgtaacg
    25141 tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc ttcaaaggag
    25201 agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat tctgctgaag
    25261 aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg ttttcttctg
    25321 tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa gcatcagcca
    25381 tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg attactacgt
    25441 cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata tttaatttgt
    25501 gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt acatctggga
    25561 ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt tctttgttca
    25621 aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc attgcattga
    25681 tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat gttccgccta
    25741 accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg attgcttctt
    25801 gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca acgctcttca
    25861 tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg atgtagattc
    25921 cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga gtttggtgac
    25981 caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg tttgtttcct
    26041 ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac tttttcaagc
    26101 acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc ctcagctatt
    26161 atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa cattcaatag
    26221 tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg tttctttatt
    26281 atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg caatccttct
    26341 tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg cgcaaagctc
    26401 aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg caagaagaac
    26461 tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt tctcgagata
    26521 tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc ttcgtattgt
    26581 ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg gttcaaatga
    26641 ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc aataccttgc
    26701 cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc tctattgtca
    26761 gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag gacgcaaaac
    26821 gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca gagcacgtat
    26881 tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa taaaccttta
    26941 cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg attgaatcac
    27001 aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt gtgtatttca
    27061 aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca ttgacgactt
    27121 ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa gaatacaaag
    27181 aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg gaaggaatat
    27241 acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta ttaagttctg
    27301 tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga caacgaatga
    27361 tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg gaacatcaaa
    27421 tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc tccgcactag
    27481 caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag attgctgctg
    27541 tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt ggtgggatac
    27601 cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac aaatgtattc
    27661 aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa aaacgaaaaa
    27721 atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg gaaaacgaga
    27781 atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa caagcgctga
    27841 aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc gccaataatc
    27901 atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa gaatatcagg
    27961 ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt aatcattttt
    28021 atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc gatcatgaat
    28081 gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa ctgacagcaa
    28141 ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga ttgggcagga
    28201 acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc ttttctagaa
    28261 aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt aaaatgggat
    28321 catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc tatttatgga
    28381 caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca ttatctgttt
    28441 aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat cgaacggttg
    28501 agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat gatgacaatc
    28561 gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac ttttgaagac
    28621 gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca gtacttcaaa
    28681 aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat ccaggaaggg
    28741 aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat ggggatttct
    28801 tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag tctgttgctc
    28861 ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag ttaacagaat
    28921 acattttgta cgctgacatg aatccagaat gacttttata caaaataaga ggttgtgaaa
    28981 aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc atatttttcg
    29041 tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc gaaaaaaaaa
    29101 agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac agtgtttaag
    29161 agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac
    29221 gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc agaacgctga
    29281 tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca aacgaaggtc
    29341 ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt tttgttcatc
    29401 atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta atttgattgc
    29461 atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg tttcagggta
    29521 tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat agtctgctgt
    29581 ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat gtgtatattg
    29641 cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa agtcaaatgt
    29701 attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc
    29761 tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt tagcagattg
    29821 gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag ttgataattt
    29881 tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag accagtcaaa
    29941 gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt cattccctac
    30001 tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag cagctaagtc
    30061 acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat tcaggtcata
    30121 agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa
    30181 aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa
    30241 acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat cacggaacca
    30301 ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc aagcacgagc
    30361 ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt ataaacgaag
    30421 caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt tgtaagcgac
    30481 gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga ttggctgata
    30541 accgattttt tccataatga aaccgatcga attaccgata actgtcgaac gcaaatgtcc
    30601 cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg ttccttgatt
    30661 cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca aaactttttt
    30721 gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt cgattttttc
    30781 aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg gcgcttttcg
    30841 gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag ctgattttgg
    30901 attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag cgtcatagac
    30961 agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt tcccttgatt
    31021 gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta gagacgagat
    31081 ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga tatcggtaga
    31141 cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc
    31201 ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg ttcaacaaat
    31261 cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt
    31321 cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat agctataaat
    31381 catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt tccttcttca
    31441 gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc gtttggtttt
    31501 gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg agcgaattgc
    31561 ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt cttttgatct
    31621 ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag caaatccgca
    31681 tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc ttctcgtcct
    31741 tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga gactgtgata
    31801 actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat gggtttaggg
    31861 gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac agtggagtca
    31921 gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg tttacttgga
    31981 tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga aaccacatct
    32041 ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca gtaaccgata
    32101 gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt ctcagaatca
    32161 gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg ggagatgatc
    32221 aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat taacgcttct
    32281 tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa agcgacaact
    32341 acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt tctagataac
    32401 aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc gtgagatgaa
    32461 tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc catgatcgct
    32521 ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt gctgcggaag
    32581 acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa
    32641 acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg atctgtgttg
    32701 tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt gatacgttct
    32761 tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac gtctgctccg
    32821 tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt ttcagcaaag
    32881 ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc aacgatacgc
    32941 atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt gtttcgacca
    33001 tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg gatagttaaa
    33061 aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca aactggaaca
    33121 ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa ttcgattccg
    33181 tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt acctaattgg
    33241 atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc agtagtaaat
    33301 gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt tccttctaga
    33361 tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa
    33421 tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc ttggaatact
    33481 gattctttca tattaaacat tctcctttag attgttttta tttaagatta aagaggaaat
    33541 cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa gtcttcacgg
    33601 acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc acttgaattg
    33661 atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata atttcggtta
    33721 taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt ccattgttct
    33781 cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc atctaatcca
    33841 agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat atcatcacca
    33901 tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc tggatggata
    33961 gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat tgcacggttg
    34021 ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa taggtggcgt
    34081 gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc tactacttta
    34141 tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg atccaaccat
    34201 acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc tgtaaacatc
    34261 gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt tcccattgat
    34321 gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa tggatagtca
    34381 tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt tgtacgaacc
    34441 ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa ctcatgaaga
    34501 ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt caagaattgt
    34561 tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta aagagtctcg
    34621 acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc aatcggaaga
    34681 tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt ttccacttct
    34741 tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa aacgtgaata
    34801 ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat atcctccttt
    34861 tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca ttaatattcg
    34921 ataattaaat aattaaacgt ttttctattt ttaagc
//
LOCUS       DAYYOA010000016        34397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000016 DAYYOA010000000
VERSION     DAYYOA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.016"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHBKA_000735"
     CDS             352..1041
                     /locus_tag="ACHBKA_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9444448.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHBKA_000736"
     CDS             1028..2236
                     /locus_tag="ACHBKA_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9444449.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHBKA_000737"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHBKA_000737"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM9444450.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHBKA_000738"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHBKA_000738"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM9444451.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACHBKA_000739"
     CDS             complement(6339..7034)
                     /locus_tag="ACHBKA_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM9444452.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHBKA_000740"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHBKA_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM9444453.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACHBKA_000741"
     CDS             complement(8233..8424)
                     /locus_tag="ACHBKA_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM9444454.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACHBKA_000742"
     CDS             complement(8445..9335)
                     /locus_tag="ACHBKA_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM9444455.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACHBKA_000743"
     CDS             complement(9322..10089)
                     /locus_tag="ACHBKA_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9444456.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHBKA_000744"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHBKA_000744"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM9444457.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHBKA_000745"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHBKA_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM9444458.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHBKA_000746"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHBKA_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM9444459.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACHBKA_000747"
     CDS             14660..15043
                     /locus_tag="ACHBKA_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444460.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHBKA_000748"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHBKA_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM9444461.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACHBKA_000749"
     CDS             complement(15987..16805)
                     /locus_tag="ACHBKA_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM9444462.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHBKA_000750"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHBKA_000750"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9444463.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHBKA_000751"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHBKA_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM9444464.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHBKA_000752"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHBKA_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM9444465.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHBKA_000753"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHBKA_000753"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM9444466.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHBKA_000754"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHBKA_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM9444467.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACHBKA_000755"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACHBKA_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM9444468.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHBKA_000756"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHBKA_000756"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM9444469.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHBKA_000757"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHBKA_000757"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM9444470.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHBKA_000758"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHBKA_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM9444471.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACHBKA_000759"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACHBKA_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9444472.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHBKA_000760"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHBKA_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM9444473.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACHBKA_000761"
     CDS             28113..30086
                     /locus_tag="ACHBKA_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM9444474.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACHBKA_000762"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACHBKA_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM9444475.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHBKA_000763"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHBKA_000763"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM9444476.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACHBKA_000764"
     CDS             32965..34257
                     /locus_tag="ACHBKA_000764"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM9444477.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10495 a         6692 c         6807 g        10403 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    34381 cagtcccttt ttccgaa
//
LOCUS       DAYYOA010000017        33009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000017 DAYYOA010000000
VERSION     DAYYOA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.017"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..1162
                     /locus_tag="ACHBKA_000765"
     CDS             251..1162
                     /locus_tag="ACHBKA_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9444478.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1418..2335)
                     /gene="rnhC"
                     /locus_tag="ACHBKA_000766"
     CDS             complement(1418..2335)
                     /gene="rnhC"
                     /locus_tag="ACHBKA_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM9444479.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2537..2974
                     /gene="zapA"
                     /locus_tag="ACHBKA_000767"
     CDS             2537..2974
                     /gene="zapA"
                     /locus_tag="ACHBKA_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM9444480.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3032..3579
                     /locus_tag="ACHBKA_000768"
                     /pseudo
     CDS             3032..3579
                     /locus_tag="ACHBKA_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            3694..6054
                     /locus_tag="ACHBKA_000769"
     CDS             3694..6054
                     /locus_tag="ACHBKA_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9444481.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6141..6455
                     /gene="trxA"
                     /locus_tag="ACHBKA_000770"
     CDS             6141..6455
                     /gene="trxA"
                     /locus_tag="ACHBKA_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM9444482.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6637..8415
                     /gene="uvrC"
                     /locus_tag="ACHBKA_000771"
     CDS             6637..8415
                     /gene="uvrC"
                     /locus_tag="ACHBKA_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM9444483.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8560..9459
                     /locus_tag="ACHBKA_000772"
     CDS             8560..9459
                     /locus_tag="ACHBKA_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444484.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9452..10714
                     /locus_tag="ACHBKA_000773"
     CDS             9452..10714
                     /locus_tag="ACHBKA_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444485.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10877..12775
                     /locus_tag="ACHBKA_000774"
     CDS             10877..12775
                     /locus_tag="ACHBKA_000774"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM9444486.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(13074..13793)
                     /locus_tag="ACHBKA_000775"
     CDS             complement(13074..13793)
                     /locus_tag="ACHBKA_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM9444487.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    13983..14030
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            14130..14462
                     /locus_tag="ACHBKA_000776"
     CDS             14130..14462
                     /locus_tag="ACHBKA_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444488.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14449..16452
                     /locus_tag="ACHBKA_000777"
     CDS             14449..16452
                     /locus_tag="ACHBKA_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM9444489.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16471..16941
                     /locus_tag="ACHBKA_000778"
     CDS             16471..16941
                     /locus_tag="ACHBKA_000778"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM9444490.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            16962..17546
                     /locus_tag="ACHBKA_000779"
     CDS             16962..17546
                     /locus_tag="ACHBKA_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFM9444491.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17561..18547
                     /locus_tag="ACHBKA_000780"
     CDS             17561..18547
                     /locus_tag="ACHBKA_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM9444492.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            18537..18848
                     /locus_tag="ACHBKA_000781"
     CDS             18537..18848
                     /locus_tag="ACHBKA_000781"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM9444493.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18870..20651
                     /locus_tag="ACHBKA_000782"
     CDS             18870..20651
                     /locus_tag="ACHBKA_000782"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM9444494.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20644..22020
                     /locus_tag="ACHBKA_000783"
     CDS             20644..22020
                     /locus_tag="ACHBKA_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM9444495.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22027..22662
                     /locus_tag="ACHBKA_000784"
     CDS             22027..22662
                     /locus_tag="ACHBKA_000784"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM9444496.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22692..24047
                     /locus_tag="ACHBKA_000785"
     CDS             22692..24047
                     /locus_tag="ACHBKA_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9444497.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24239..25633)
                     /locus_tag="ACHBKA_000786"
     CDS             complement(24239..25633)
                     /locus_tag="ACHBKA_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9444498.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25725..26696
                     /locus_tag="ACHBKA_000787"
     CDS             25725..26696
                     /locus_tag="ACHBKA_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM9444499.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26693..27475
                     /locus_tag="ACHBKA_000788"
     CDS             26693..27475
                     /locus_tag="ACHBKA_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM9444500.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27644..28015)
                     /locus_tag="ACHBKA_000789"
     CDS             complement(27644..28015)
                     /locus_tag="ACHBKA_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9444501.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28149..28592
                     /locus_tag="ACHBKA_000790"
     CDS             28149..28592
                     /locus_tag="ACHBKA_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9444502.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28635..29444)
                     /gene="yidA"
                     /locus_tag="ACHBKA_000791"
     CDS             complement(28635..29444)
                     /gene="yidA"
                     /locus_tag="ACHBKA_000791"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM9444503.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29463..30830)
                     /locus_tag="ACHBKA_000792"
     CDS             complement(29463..30830)
                     /locus_tag="ACHBKA_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9444504.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30814..31644)
                     /locus_tag="ACHBKA_000793"
     CDS             complement(30814..31644)
                     /locus_tag="ACHBKA_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM9444505.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31807..32244
                     /locus_tag="ACHBKA_000794"
     CDS             31807..32244
                     /locus_tag="ACHBKA_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM9444506.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32320..32940
                     /gene="rpoE"
                     /locus_tag="ACHBKA_000795"
     CDS             32320..32940
                     /gene="rpoE"
                     /locus_tag="ACHBKA_000795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM9444507.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        10751 a         5662 c         7093 g         9503 t
ORIGIN      
        1 tgttccagga agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa
       61 actatttatt gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca
      121 aaaatgaaaa aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc
      181 ccttttttgc gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg
      241 agagtcaaga atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat
      301 ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac
      361 gaacagtacg gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat
      421 caaaatcgga aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc
      481 aactataaac ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat
      541 caagacaaat gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa
      601 gaatatacaa acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca
      661 tggcggaaat gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac
      721 attaagtaca gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt
      781 gacccatact acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa
      841 aaaggcagat gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac
      901 tggaatcacg acatattatt actatggaca agaggaaaca ttagcacaga caattgctga
      961 ctccttcact gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct
     1021 tcgagaaaat cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga
     1081 tttaaaggaa ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc
     1141 attgaccact tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt
     1201 tttcgtttgg ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag
     1261 gttgtgagcc tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca
     1321 tgtttgatga ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg
     1381 aacaagggac tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag
     1441 ctttttctgt attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt
     1501 tctccaatag ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga
     1561 ttccagcttc agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag
     1621 aagcagcagc aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt
     1681 gtaattgatt tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat
     1741 cgatcaaaat cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt
     1801 gattgtgcaa agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt
     1861 tttctggaga taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct
     1921 ggatgatttg gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg
     1981 caagctgatc acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat
     2041 ttccgacctc atcactacct aaaacggata attgactgat attttttggc aaagaagagg
     2101 ctgtttttgc tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg
     2161 ctgcattcgt accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt
     2221 ctttttttgc agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt
     2281 atgctttcat ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat
     2341 tctcctcgtt tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga
     2401 ttgcgagaca tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat
     2461 ttgtggagaa cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata
     2521 ggaggaaatg attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca
     2581 aacatatact attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat
     2641 caatgaacag ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc
     2701 aatcttgatg gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga
     2761 gctggaagaa gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa
     2821 tcgtgtgaaa cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg
     2881 tcaagcagat cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg
     2941 aaaaggacaa attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat
     3001 ttaaaaaagt tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt
     3061 gctaattgcg tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat
     3121 tggatatgta ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt
     3181 ggaattatac attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca
     3241 ggtatttgct tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt
     3301 gctaattggc tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt
     3361 gccgatcttg agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta
     3421 cgttactata tttttgattt tgcagttact tgcctttgtt ccattagtgt ggtccaaaat
     3481 caattcggcg agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac
     3541 aagatgtatg agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata
     3601 tttcgttttt tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag
     3661 ctcttttttc atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg
     3721 gaatttgaaa aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa
     3781 gaactggcag aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa
     3841 actgaggatg gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa
     3901 aatatccgtc cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt
     3961 gcacaagttt ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt
     4021 tctgacagtg aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc
     4081 cctgttttga gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac
     4141 gcttctcctg agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt
     4201 gaacagttag atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc
     4261 accatgcgaa atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc
     4321 ggtgtagtcc acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg
     4381 gtggatttga ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt
     4441 attttaagtg aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct
     4501 tatgtgattg gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa
     4561 gccatcgtgc cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca
     4621 ctgatcgatc aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc
     4681 atcgtcatta caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg
     4741 ttacaactga tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt
     4801 atttttgagg aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg
     4861 accttttctt ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt
     4921 ctggtattat ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca
     4981 atcgcaatct tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat
     5041 cctgaattga aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc
     5101 gatgtggata cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat
     5161 gcgtttgaaa tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag
     5221 ttgatgaatg atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg
     5281 aaaatggcag agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt
     5341 cacgatgatt tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa
     5401 aaagcaaaga aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc
     5461 attgctgata ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag
     5521 ttgatcgatg caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat
     5581 aaagtattga agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt
     5641 acgacttacg gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa
     5701 atcggtatat taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa
     5761 gagccgacac aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat
     5821 caattggacc ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg
     5881 gattctgcca ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc
     5941 gcacttcgca agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag
     6001 tttgcaccgg caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta
     6061 ctaaaggtaa gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt
     6121 taaaaatgga ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa
     6181 cagataaagg acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc
     6241 aagcaccgat tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta
     6301 aaatggatgt ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa
     6361 cattaatgtt gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag
     6421 aacaactacg tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag
     6481 ggtgggactg aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa
     6541 ttaatatttt ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct
     6601 ataataactt taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa
     6661 ttagctttac ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc
     6721 atttatgtcg gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc
     6781 cataatacga aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca
     6841 gaatcgaata tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa
     6901 tacaacatta tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa
     6961 tatccccgtt tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca
     7021 tatcccgatg tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg
     7081 agaaaatgca aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt
     7141 ctgtgtcctt attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg
     7201 aaaaagtttt taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa
     7261 aaagctgctg aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc
     7321 attgagacaa tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta
     7381 tttggttatg cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga
     7441 aagctaattg aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc
     7501 ttgacttacc tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg
     7561 attcctgata cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa
     7621 ccaaaacgag gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct
     7681 ttgaatgaac ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa
     7741 cgtttaggaa acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct
     7801 aatatcatgg ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca
     7861 agaaaagaat atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca
     7921 atgagagaag tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct
     7981 gatttgatct tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa
     8041 aatcaattag gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca
     8101 aatgagttgc tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag
     8161 tttttcttac tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg
     8221 cagctacgca gtaaaaacag ttttgcttcc cgattagatg aaattgaaag gttaggacca
     8281 aaacgaaaaa aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg
     8341 acagaagaat tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa
     8401 ttacatcagg aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat
     8461 gtacaacaaa aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt
     8521 ctaaggatat atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt
     8581 gttaaaactt ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga
     8641 aaaatcatgg ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc
     8701 ttggattttt tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag
     8761 aaagattatg atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg
     8821 attcaagacc aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa
     8881 ccaaaaatcg atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta
     8941 aaatctttgt ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa
     9001 cctaaattga tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg
     9061 ctaaaagatg gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac
     9121 aatatggata atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg
     9181 gttttgaatg gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg
     9241 gaatctggtt taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt
     9301 catgaagaca atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt
     9361 actcgtgcga ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa
     9421 gagattttca aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga
     9481 tgtctattta aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat
     9541 catgctgggt atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa
     9601 aatcggcgta ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga
     9661 tgattttgag ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga
     9721 aaaaatcgat gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta
     9781 tagcgaaaat tcattaggac aaagtacaca actactgata caacaacaac tgactggctt
     9841 gcaatcgatg cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag
     9901 ccaacaagca agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt
     9961 tggagaagat aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt
    10021 cattattatt ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac
    10081 gcggatcatg gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact
    10141 aacaggtgtt ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat
    10201 aggatacagt caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt
    10261 ggataacttg attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt
    10321 gattttttct gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc
    10381 aaaagcaatt ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact
    10441 aggagcttca gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc
    10501 ttcatatatt atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat
    10561 ttccttgttg gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta
    10621 caaatcgaat gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat
    10681 ttcaattatg agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct
    10741 ggacatttat tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat
    10801 tggatatata tattcattga aaatagagca taacaacttt ataatgttga gtataccaat
    10861 ttttggaggc gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac
    10921 ggaagatgtc atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac
    10981 agagttattt ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat
    11041 cggttcagga aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac
    11101 ggagatcagc caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg
    11161 gtacaaaaaa caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac
    11221 gacagtcaaa tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat
    11281 cattgaggaa ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa
    11341 aatcattgaa caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca
    11401 gacgatccaa aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg
    11461 aactcaagca gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg
    11521 ggggaatcat gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac
    11581 gcttttacgg attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt
    11641 aaatttacgt ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac
    11701 ccctaaaaac aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca
    11761 agcactttct atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt
    11821 taaaatggac gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt
    11881 agaaggtcag ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc
    11941 taaaaaattc ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca
    12001 ctcgttaaga atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac
    12061 gaacaatcag catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt
    12121 agcatttgaa ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca
    12181 gataactgaa agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg
    12241 gtatgcgtgg agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga
    12301 gcgctatttt ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt
    12361 gcgagatgaa gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg
    12421 ttcagcaatc atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa
    12481 agcaaacgga aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac
    12541 cgggattgct ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta
    12601 tccgttcttg cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa
    12661 agtgatcgag cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt
    12721 acagcaacag attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa
    12781 atgctagcca acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt
    12841 ctccttcgga attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc
    12901 tctaatactc aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc
    12961 caactcctcg gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg
    13021 ctccttgtga ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat
    13081 tcattcacaa agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc
    13141 ggatgccatt ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg
    13201 agtccatcgt tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga
    13261 tgatgatagg aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa
    13321 acgattttga cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg
    13381 gaccattttg gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat
    13441 agttgaagtc ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc
    13501 aatgcctgtt cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct
    13561 tccatgtaaa gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc
    13621 tcaattagtt gaggcgctga atctggagac atgactggca gaataagagg taatccttga
    13681 gcatctaaaa ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa
    13741 gtatctgtcg ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt
    13801 ccccctaaag tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg
    13861 cccctatact tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat
    13921 tcatgacgat agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa
    13981 aaggttgggc gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg
    14041 aaggggcctg cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag
    14101 catgtacctc ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa
    14161 tccgaaaagc agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga
    14221 cctatgcaga agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc
    14281 tgaaacagtt ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa
    14341 tgaaattgaa agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc
    14401 atcagagtca agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact
    14461 aagatggaaa aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca
    14521 gtacaaggaa tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag
    14581 tgggtagaaa aatattttcc agataccacg attattgata aagaagcaaa attaagcgct
    14641 ttaggcaatc ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat
    14701 agccgtcaaa agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca
    14761 aattattccg aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag
    14821 tgggaacagc taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat
    14881 tggcaacagc tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg
    14941 gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac
    15001 gtttatatgg aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta
    15061 aaaaaatcag ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg
    15121 tatccttatg aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt
    15181 gttcacgagc agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa
    15241 gaatttgaat gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa
    15301 cggtttatcc atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa
    15361 cagaatttga tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc
    15421 gaaacaccaa actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg
    15481 cttatcgaac cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta
    15541 gatccaacgc cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat
    15601 gttggttacg gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta
    15661 ccccgcggga tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc
    15721 tggggattga tctacagttc atttttcgga caggcattac caaaagaact atttggtatc
    15781 cgcttgcctt tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc
    15841 gtgattttcg gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc
    15901 aggaggaaag attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt
    15961 gggatcgtta ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt
    16021 ggcgcggggc tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg
    16081 ccgtctaaag tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat
    16141 atcggtgatt tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt
    16201 ggcgcggcat tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg
    16261 atccttttga tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat
    16321 gtccatggtg cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga
    16381 cgtgcctttg acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa
    16441 aacaataaat aattggagga tttttataca atgatggatt acttaattag tcaaaatggt
    16501 ggaatggtat ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca
    16561 gctaaaggtg ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa
    16621 aaattcggac aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc
    16681 gttatcgcct ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg
    16741 aactttctag gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa
    16801 gggaaagttg cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa
    16861 ggaatcattt ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc
    16921 ctattagtat tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa
    16981 tcatctctca gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc
    17041 gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa
    17101 aacaaaaaga aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc
    17161 aacaagtaga agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt
    17221 ttgcagatgc aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca
    17281 aaaattcatt gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat
    17341 cagcagctta tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga
    17401 tggttttaag tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag
    17461 ttcagtataa ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg
    17521 agattgccaa tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa
    17581 tcccttgatt cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat
    17641 tcaaacaaat tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata
    17701 tatttatgac ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct
    17761 atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg
    17821 ctatactttc cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga
    17881 ccatttgtat atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac
    17941 gcaagtttca aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta
    18001 ttttgaagag tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac
    18061 agagcagcga cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc
    18121 ttttattgac ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata
    18181 tgggttcatg acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa
    18241 ctttgtagat aaagatatgg aagattacac gaattttcta ttagagacag attatgctga
    18301 ttttctgcgc cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa
    18361 agatgattat ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc
    18421 actactagcc tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat
    18481 cggcaaaaga agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga
    18541 cgcataagat cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct
    18601 ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa
    18661 agaatgagta tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga
    18721 tcgaacgtta caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat
    18781 cattagggat agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata
    18841 tcttatagta tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg
    18901 gacctcttgt aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg
    18961 gagatctagg agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag
    19021 tatatgaaga gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt
    19081 tatctgttga attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat
    19141 tagatacctt tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg
    19201 cattggacca tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga
    19261 gtgctggaga tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg
    19321 tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg
    19381 aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat
    19441 ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca
    19501 ctggacagcg agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac
    19561 ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg
    19621 tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa
    19681 atgaattccc tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat
    19741 tgattgctaa tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa
    19801 tcacaattgc ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca
    19861 cttcacgatg ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg
    19921 atgaaggtta tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa
    19981 gagtcattgc attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat
    20041 ccccgtctgg aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag
    20101 tattctgggg tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga
    20161 tacaaagcta ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag
    20221 ctgactgggc atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga
    20281 atgaaatcgt gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag
    20341 aagtggcaaa atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata
    20401 cattcacttc aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag
    20461 aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat
    20521 cgattcgtga aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag
    20581 atcaaattat tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga
    20641 gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa
    20701 aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt
    20761 cgtcgtggac aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga
    20821 acaagtggta tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta
    20881 ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac
    20941 ggaccagaga tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta
    21001 gctcgggatt atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat
    21061 acattagttc gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa
    21121 ttagctgctc agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta
    21181 gtctttgctg ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt
    21241 caaacgggcg caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc
    21301 gaacgtatcg ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag
    21361 ggaatgcatg ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag
    21421 atttctgcag cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg
    21481 aacttagcta ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact
    21541 caaattccga ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact
    21601 ggttatatta cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag
    21661 ccaccaattg atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga
    21721 aagacaagag aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga
    21781 aaacaggcaa aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag
    21841 atctatgcga agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg
    21901 aaccgttcga ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga
    21961 accgaattaa aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga
    22021 gttctgatgg cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa
    22081 cagttgacaa ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg
    22141 cgtcaattca tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct
    22201 atgacagaag caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc
    22261 gaagaattat ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc
    22321 atgagtgtaa aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct
    22381 ttggaatacg ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact
    22441 accctgcttc caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg
    22501 agtgaaatcg aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa
    22561 ttagaagaaa cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta
    22621 acaaggttga tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa
    22681 agaggaaaac gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc
    22741 ttctcgcatt aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat
    22801 tttttagtaa tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg
    22861 ctgtctgtgt aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc
    22921 aatttttgat catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc
    22981 tatgctttgc attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag
    23041 cgctgaatct agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg
    23101 cctttgttat ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg
    23161 gctggttgaa aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag
    23221 ggtttgattt gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg
    23281 tggtatctgc tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg
    23341 aatatcatca gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta
    23401 cccttttatt aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag
    23461 tggaagggaa tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa
    23521 cggctggata ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga
    23581 tgtttttgat gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta
    23641 cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag
    23701 catttggccg taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta
    23761 cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa
    23821 cttctggcat cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta
    23881 cgatgggatt gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt
    23941 acattggacg agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa
    24001 cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga
    24061 cagaaaccta gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata
    24121 ctttaagtgc aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta
    24181 gtgcctcttt tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt
    24241 ataattcata tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa
    24301 ggttattaga ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc
    24361 gtctggaatg ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt
    24421 caatctcagg atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta
    24481 ctttgaaaag agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta
    24541 agatcctatt tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag
    24601 aagttttcac atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa
    24661 ctacaagata agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga
    24721 ttgtttggtt aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg
    24781 ttaaaaatga atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg
    24841 ctaattgctc tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca
    24901 taagaaagac accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca
    24961 ttccttgctc gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa
    25021 tgaaattagc gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag
    25081 aaagtgtgat agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg
    25141 gaatgactcc ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt
    25201 tgggatcttg gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa
    25261 acgggacaac gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat
    25321 gttcggttac ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg
    25381 tgccacttcc aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc
    25441 caacggtagt aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag
    25501 tgtttttctg taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat
    25561 aataaggacg cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag
    25621 aattaatagg cattttctcc actccttaaa attgtatacc atacaatttt accattgatt
    25681 gtattgattt tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt
    25741 ttgaggaagt agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt
    25801 tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg
    25861 acggaaaagg caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt
    25921 ttggcacaaa agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt
    25981 taggagagat tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa
    26041 atgcagtcac ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa
    26101 atccgcatgc agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga
    26161 ctgcgatttc taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa
    26221 gacgaaatac ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg
    26281 caatatcagt agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta
    26341 aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca
    26401 catttactcc tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg
    26461 tagatgatta ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag
    26521 gagatttaga ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa
    26581 cattttataa aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag
    26641 atctttgttg tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt
    26701 agattattac gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt
    26761 ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa
    26821 tttatctgaa acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac
    26881 gccagatcga gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt
    26941 taactattac aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt
    27001 tgtgacaaga attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt
    27061 gccgatattg tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc
    27121 acgtgattta tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt
    27181 tacagctatc aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca
    27241 aaaagacttt gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg
    27301 tggatcagca cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt
    27361 gtcagcttat caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg
    27421 tgtgataata ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct
    27481 ttcaaatgaa ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt
    27541 aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg
    27601 tgtttgtttt ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt
    27661 cgattttata accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc
    27721 cttttaagtc agtaatctca agaccggcag ctttatgttg ctcgtgtaat ttttctagat
    27781 catcagctgc tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat
    27841 agttgtaagt caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa
    27901 atttgttttc tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa
    27961 attcgattga tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt
    28021 catctctcct tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg
    28081 tgcttgttca tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga
    28141 aactattcat ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc
    28201 gttatgcagc atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa
    28261 atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga
    28321 ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag
    28381 aagctgatga gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt
    28441 tctttgttgc tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt
    28501 ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag
    28561 tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc
    28621 agttttttta tttattacag tacatatttt ttgattgctt cagctacacc gtgctcgtcg
    28681 tttgatgaag tgatgacatt tgctgcttct ttgacaagag aaacggcatt tcccattgct
    28741 actcctagac cagcatattc aatcatggat aagtcgttct cgttatctcc gatagccatg
    28801 atttcatctt gatcaatacc aaggtgctgt gcgagattcg caactgccgc acctttactt
    28861 gcttctttat ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt gtatttatca
    28921 cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa tttctggttc gtcgatcatc
    28981 atcattttga cgatgttcat gtctggtgtc atttcttcag gtgttcgata ttttagcggc
    29041 atcttgacta aacttgcttc gtggatcgta taagcgctga tatctcgatt aggcgtgtag
    29101 atcgctttgt catcgatcga atggagatga ctgccgacac ggcgtgccat cacttcgatc
    29161 tctaggaaat cttcatgtgt cagtccgtga cgggcgatga ttttacctga tttcgtttgt
    29221 tgtacgagtg ccccgttata agtaataacg taatcattat cttgaaaaag gttcaactcg
    29281 tttaactgtt cctggacacc tggtaaggga cggccagtgc ataaaacgat cgaaacacct
    29341 tttgcaacag cagcggcaat cgcttctttt acttcttttg ttacttctct tttctcattt
    29401 aataaagtac catcgatgtc gatagcgact agtttgatag acataatttc ctccaatata
    29461 ttttaattta tttcaatcaa tgcgccatta cggatatggc tggcaaattc ttgataagtt
    29521 tcgtcaaata aatcataatg atcacgcaag gacggatcaa ccatttcttt tggaaagaaa
    29581 aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa caagctgact aacctgtgat
    29641 aattctatca aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt
    29701 tctgggcgat aaaaatcata aggtagatcg tagctagaat tcacagcagt gtaataaaca
    29761 ggattatatc cgaccttttc tactaacaaa gtgagttctt ggactaattt tgaatcccgg
    29821 ttgccagaaa aagtagcaga tttcagtggt ttacgattca aaaaacgttt agctaaatca
    29881 tttaaaatag gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa gaccccatca
    29941 tcaagtttga ggtattcttc taaagtaaag cttccgtcaa gaaatggcag aagtaattga
    30001 gaatggagtt caaaactgct ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga
    30061 tgctccaaaa ttacttccat accacgggaa acaggatgga agtacacttg cacatacatt
    30121 tgatatcggc taacgatata atcttccact gcatgcatgc cactcatgga aaaagcgatc
    30181 ccgccttcat aaggacgaat cacacgtaat attcgggtaa gatcaaatgt tccatattct
    30241 gttccagtga aataagcatc ccgaagcaaa tagtccattc gatctgcatc gatctgacta
    30301 gaaatcattt ggacaacttg tgggttgggg taagtttttt ggatcacact agctaccttt
    30361 tcaggaaaac cttcttctac tcggttaaga atctgataca cttctgtttc tggagaagtg
    30421 atgatctcta cagtgatggc ttcatgattc gtatggaaga tatgttcaaa tgtatgagag
    30481 taaggaccat gaccgacatc atgcaacagt gccgcacata aagtcaccag ccgttcctga
    30541 tcatcccagc ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa gatatcgcag
    30601 attcgtcttg aaatctcata aacgcctaat gaatgagtaa aacggctatg ttctgctccg
    30661 tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg gacttctttc
    30721 gaattgatca aatctaagat tacttgatgc tggacatgga tatagttatg gactggatca
    30781 cgaaaaactt tttccatagg cagcatttga tatttataag gggttgtcat aatgtagctc
    30841 ctttatgtta ttacgttgtt ccattctctg gatttggttg ttccattgct ccagctggat
    30901 ttcttttttt agccagtcgg tagaatcctg taaatcactt gcagaataca gcttttcaaa
    30961 agcttttgca aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc
    31021 aagtgttgcc attgtagcag gatccactgc aggatagcca tttgtaccaa agtcttcttt
    31081 taaagcagat tgataaaatc gcttcatgac ttgcccgcgt ttttcctgat taccgttgac
    31141 acttaagtac atcataacag aaatcccatt tttgatccga cgctgagcga taccggcaaa
    31201 ttttttgcca tggatgctta aatcataagt tccaggacaa taagaatggg tgatttcgaa
    31261 tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat aggagcattt gctcgtaagc
    31321 gtcatctatg gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat
    31381 tccttcatct gatacgatcc ctaatccacc tgaatttctg atcactggag cgtaacctgc
    31441 atctttcagt gtttggatcc cgtcacttag aaaaggagtt cgaacatcct tcatacctaa
    31501 gatgactgtg cagtcatatt gccagaaatg taaaaagatt tgctggtgtt ctttagaata
    31561 ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg
    31621 atctagtcga atcgcagggt tcattcaacc ggctcctttg tataaattca tattttctac
    31681 aatattatac tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac
    31741 aagttggttg acaaaacaaa agaaactagg aacaatgtag ctatgagaag taaaggagaa
    31801 tgaataatgg aattatcaca aggaacaccg atttcaatca aattgcgtac aaaagtaaaa
    31861 cagcaagggg aagtgcaaga tttttatttt gatttgaaag ggcaaatggt gacaattggc
    31921 gatacgctgt acatacgtta taaagagatt caggaggaga cgggcgatga gattcctgta
    31981 acgatcaaat tagttccaga tggtcatgta cagctgatcc gtgcaggaga aatgcggatg
    32041 cgtttgaaat ttggctataa agaacggtta gaaacatcct atcgaacacc atacggtatg
    32101 atccagattg ctacttttac gaaagaactg catgttagtt tgaaagaccg tcccacagga
    32161 gggaaggttc gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg cgaatattat
    32221 ttgaccttgg attttacagc gtaaatataa aagaaaagtt tgattttttg tgtctgattt
    32281 tgtatgataa ggagtagact gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa
    32341 ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac acgcgatctt agaacaacgt
    32401 ggcgatgtca tggatttttc tgatttagct aaccagatcc aaaactatct tgggaaatca
    32461 gacagtgaaa ttcgtgattc actcgcacaa ttttatactg acttgaatat tgacggcagc
    32521 tttatctcat taggggataa tcgttggggc ttgcgttcat ggtatgctat cgattcgatc
    32581 gatgaagaag taaaccatgg aatggacgaa gaggatgaag atactccacg tcgccgtaaa
    32641 cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg cgatcgatta caatgacgat
    32701 gacccagaag atacagatct gacagaagaa gatgacgatg atctgtttga tgacgatgat
    32761 gatgaagatg aagaaatcgc tgcttataat tctgacttgc aagaaatcgg agcagatgac
    32821 ccgacagatg atgaagaaga cttgccagga aatatcgaag aagatttaag tatcatagac
    32881 gaagacgatg atgatgaaga ttatgaagag gaagaatttt ctgactctga aaaagaataa
    32941 acattatttt cttacgagag gttgtgacag agacgatcag cctatcagaa taaggaggtg
    33001 tgacacgat
//
LOCUS       DAYYOA010000018        32294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000018 DAYYOA010000000
VERSION     DAYYOA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.018"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(231..611)
                     /gene="yajC"
                     /locus_tag="ACHBKA_000796"
     CDS             complement(231..611)
                     /gene="yajC"
                     /locus_tag="ACHBKA_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM9444508.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(708..1853)
                     /gene="tgt"
                     /locus_tag="ACHBKA_000797"
     CDS             complement(708..1853)
                     /gene="tgt"
                     /locus_tag="ACHBKA_000797"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM9444509.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2071..2769
                     /locus_tag="ACHBKA_000798"
     CDS             2071..2769
                     /locus_tag="ACHBKA_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9444510.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2937..5576)
                     /locus_tag="ACHBKA_000799"
     CDS             complement(2937..5576)
                     /locus_tag="ACHBKA_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9444511.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6195..7226)
                     /gene="queA"
                     /locus_tag="ACHBKA_000800"
     CDS             complement(6195..7226)
                     /gene="queA"
                     /locus_tag="ACHBKA_000800"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM9444512.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7419..8594
                     /locus_tag="ACHBKA_000801"
     CDS             7419..8594
                     /locus_tag="ACHBKA_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9444513.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8598..9239
                     /locus_tag="ACHBKA_000802"
     CDS             8598..9239
                     /locus_tag="ACHBKA_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444514.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9236..10153
                     /locus_tag="ACHBKA_000803"
     CDS             9236..10153
                     /locus_tag="ACHBKA_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9444515.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10150..10821
                     /locus_tag="ACHBKA_000804"
     CDS             10150..10821
                     /locus_tag="ACHBKA_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444516.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11362..11850)
                     /locus_tag="ACHBKA_000805"
     CDS             complement(11362..11850)
                     /locus_tag="ACHBKA_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM9444517.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(12051..12317)
                     /locus_tag="ACHBKA_000806"
     CDS             complement(12051..12317)
                     /locus_tag="ACHBKA_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9444518.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12317..12580)
                     /locus_tag="ACHBKA_000807"
     CDS             complement(12317..12580)
                     /locus_tag="ACHBKA_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9444519.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13101..13241
                     /locus_tag="ACHBKA_000808"
                     /pseudo
     CDS             13101..13241
                     /locus_tag="ACHBKA_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13383..15206)
                     /locus_tag="ACHBKA_000809"
     CDS             complement(13383..15206)
                     /locus_tag="ACHBKA_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9444520.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15804..18509)
                     /locus_tag="ACHBKA_000810"
     CDS             complement(15804..18509)
                     /locus_tag="ACHBKA_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM9444521.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18815..19762)
                     /locus_tag="ACHBKA_000811"
     CDS             complement(18815..19762)
                     /locus_tag="ACHBKA_000811"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM9444522.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19952..20221
                     /gene="rpsN"
                     /locus_tag="ACHBKA_000812"
     CDS             19952..20221
                     /gene="rpsN"
                     /locus_tag="ACHBKA_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9444523.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20397..20759)
                     /locus_tag="ACHBKA_000813"
     CDS             complement(20397..20759)
                     /locus_tag="ACHBKA_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9444524.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20770..21891)
                     /gene="alr"
                     /locus_tag="ACHBKA_000814"
     CDS             complement(20770..21891)
                     /gene="alr"
                     /locus_tag="ACHBKA_000814"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM9444525.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21907..22257)
                     /gene="acpS"
                     /locus_tag="ACHBKA_000815"
     CDS             complement(21907..22257)
                     /gene="acpS"
                     /locus_tag="ACHBKA_000815"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM9444526.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22387..23898)
                     /locus_tag="ACHBKA_000816"
     CDS             complement(22387..23898)
                     /locus_tag="ACHBKA_000816"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9444527.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24220..25572)
                     /gene="murF"
                     /locus_tag="ACHBKA_000817"
     CDS             complement(24220..25572)
                     /gene="murF"
                     /locus_tag="ACHBKA_000817"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM9444528.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25725..26801)
                     /locus_tag="ACHBKA_000818"
     CDS             complement(25725..26801)
                     /locus_tag="ACHBKA_000818"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM9444529.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26997..28742
                     /locus_tag="ACHBKA_000819"
     CDS             26997..28742
                     /locus_tag="ACHBKA_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM9444530.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(29022..29459)
                     /locus_tag="ACHBKA_000820"
     CDS             complement(29022..29459)
                     /locus_tag="ACHBKA_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM9444531.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29443..30816)
                     /locus_tag="ACHBKA_000821"
     CDS             complement(29443..30816)
                     /locus_tag="ACHBKA_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444532.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31138..31626)
                     /locus_tag="ACHBKA_000822"
     CDS             complement(31138..31626)
                     /locus_tag="ACHBKA_000822"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9444533.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31648..32250)
                     /locus_tag="ACHBKA_000823"
     CDS             complement(31648..32250)
                     /locus_tag="ACHBKA_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9444534.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9237 a         6889 c         5953 g        10215 t
ORIGIN      
        1 gtaaacactt ttttgtgatt tttttcacag ttatttttaa atataaaatt gctttattta
       61 caggtgtttg tatagaaaaa tatataaagt tatttattat tatttatatg acacacccga
      121 ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat
      181 gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct
      241 tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca
      301 gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt
      361 ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca
      421 actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg
      481 actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt
      541 tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt
      601 aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa
      661 aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt
      721 gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta
      781 tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg
      841 taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca
      901 cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg
      961 gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga
     1021 gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc
     1081 aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt
     1141 gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg
     1201 attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct
     1261 tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct
     1321 ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa
     1381 ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt
     1441 tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg
     1501 aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg
     1561 cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct
     1621 tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca
     1681 gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact
     1741 ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg
     1801 ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc
     1861 cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt
     1921 ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt
     1981 atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt
     2041 ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa
     2101 gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca
     2161 ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc
     2221 atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga
     2281 ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg
     2341 acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt
     2401 cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc
     2461 tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat
     2521 tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta
     2581 ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg
     2641 ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat
     2701 ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa
     2761 gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg
     2821 agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt
     2881 cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat
     2941 tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt
     3001 acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat
     3061 agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc
     3121 atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt
     3181 acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg
     3241 acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa
     3301 ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct
     3361 cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct
     3421 ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt
     3481 tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc
     3541 tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa
     3601 cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct
     3661 gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct
     3721 ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt
     3781 tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga
     3841 gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt
     3901 gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca
     3961 tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct
     4021 ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt
     4081 tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca
     4141 atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg
     4201 cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt
     4261 tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt
     4321 actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt
     4381 acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc
     4441 gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa
     4501 tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt
     4561 tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact
     4621 gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt
     4681 gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa
     4741 gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc
     4801 gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa
     4861 ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc
     4921 atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat
     4981 accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt
     5041 tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg
     5101 atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact
     5161 accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt
     5221 ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc
     5281 aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt
     5341 tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta
     5401 aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat
     5461 ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct
     5521 ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta
     5581 ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt
     5641 tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg
     5701 cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc
     5761 atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt
     5821 acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga
     5881 tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg
     5941 taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa
     6001 acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag
     6061 ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag
     6121 agctcggctt tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc
     6181 ggcttttcat aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc
     6241 tcttcaatcg catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact
     6301 aacatgacta aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg
     6361 aattcatagc caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca
     6421 ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt
     6481 ttcccgccat ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag
     6541 aattcactat gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct
     6601 aaccctacat gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt
     6661 aattctttcg taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca
     6721 tagacagttt gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc
     6781 atttctccca atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc
     6841 cgtccgccgt gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag
     6901 attcttgtcc caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct
     6961 tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat
     7021 aaacgagcag gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct
     7081 tcaatgattt catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt
     7141 aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca
     7201 aaatcaaaat cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat
     7261 tctatcatgt ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt
     7321 tcctaaataa aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata
     7381 gatagttctt cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt
     7441 caaaaattta taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg
     7501 gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga
     7561 tcaaccgaat gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag
     7621 aaattgatcc agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga
     7681 tgcctcacat gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc
     7741 aagaagaacg gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga
     7801 tgttagatcg ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc
     7861 gtgcccttgc agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc
     7921 caatcactcg tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa
     7981 cgatcgtatt tgtaacccat gacatggatg aagcgattaa actagcaagc cgtatcgcca
     8041 tcatgagtga aggccgtgtg atccaatttg atacaccaga aaatatttta cgtcatcctg
     8101 caaacgattt cgtagaagag ttgattggag aagatcgcct actacaagcc aagccaaatg
     8161 cgacaagagt cggcgaagtt atgctaaata cagcaatcac gattacaccg gaaaaatcat
     8221 tacaagaagc aatcaaactg atgagggaaa aacgtgtaga tacgttgtta gttaccgata
     8281 attcgaatgt attaaaagga tttatcgatg tagaaacgtt gaataaaaaa cgaggaaaag
     8341 tatccagtgt cggcgacatt ttgaataaac atgtttttta cgtaaaagaa tccgcctatc
     8401 ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa atacgtgccg gtcgttgatg
     8461 aacagaacaa agtcgtaggt atcctgactc gtgcatcatt agtggatatc gtctacgatg
     8521 tgatttgggg agaagatccc gcaactactg atgttgcaga gtcagctact cctgaagaat
     8581 ctaaggaggt ctgacctatg cagcagtttt ttcatcagca cggttcagaa attctctcaa
     8641 aaggttttga acatctttat atttcaggac ttgctttgat cattggtatc atcattgctg
     8701 taccgcttgg tgtcttactg acacgttttc caaaagctgc cacgattgtg attggcttaa
     8761 ccagtgcctt gcagacaatc ccttctcttg ctttattggc attgatgatc ccgctatttg
     8821 gcgtaggtaa attacctgcg attatcgcac tctttattta ttcattattg ccgattttac
     8881 gtaatacgta cattgggatg aaaaatgtcg attggaatta tcgggatgtg gcaaaaggca
     8941 tggggatgac tgagttccaa tccatttttt ccgttgaatt gcccattgct atgccaacga
     9001 tcatggctgg tattcgttta gctgccgttt acgtgattgc atgggctaca cttgcctctt
     9061 atatcggcgc tggtggttta ggcgatttga tttttagtgg actgaataat taccaaccag
     9121 atctcatttt ggcaggtacg atccctgtaa caattttagc actattggct gattggcttc
     9181 taggtctctt ggaacaccgg ctgacaccga tcgcacttag agaggagaat gaagaatgaa
     9241 acgtatacta aaaatgacca tggttctttt ctctcttctt atacttgcga gttgttcctt
     9301 ccctggtctt gccagcaata cagatgaaga caccatttcg atcacaggtg gaatcacttc
     9361 agaagcacag atcttagcaa gtctagttgc cggaatggtt gaacattata cagataaaaa
     9421 taccgcaatc atcaacaatc tggctactac aactatcaac catcaagcga tgatgaacgg
     9481 agatgcttca atttcagcgg ctcgctacac aggaacagat ttaacgacta cattgaacct
     9541 tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat ttgagaaaag
     9601 atacggtcaa acgtggttcc cgtcctatag ttttgaaaat acgtatgtct ttcttgttcg
     9661 aaaagacacc gcacaaaaat accatctatc taaagtcagc gatctgaaaa atgtagcgga
     9721 tgaattagta gctggtgtag atacttcttg gatcaatcgt aaaggagacg gttatgacgg
     9781 cttccaagaa acttatggct tctccttcaa ttcgatatta cctatgcaga ttggacttgt
     9841 ttatgacgca gtcgaagcag gaaaaatgga tattgttctt ggctactcta cggatggacg
     9901 gatcgccagt tatgatctag tcatgttgga agatgatcgt caattcttcc caccatatga
     9961 tgctgctcca gttgtagaca acaagatatt agctgatacg cctcatttaa aagaagcctt
    10021 aaataagtta ggtcatacca tcagtacgga aaaaatgcag gaattgaact acgaagcaga
    10081 taataatcta gtggaaccat cagtagtagc acaacgtttc ttacaagaaa accactattt
    10141 tgaagggaag tgaagcaatt ggataaaatg aatgtctttc aacaattttt ctattatttc
    10201 caggaaaatg gctcctatat ctttgcacaa tttatccgtc acttcctgat ttctatctat
    10261 ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga tcatgatttc taggagacgt
    10321 aaattagcaa actgggtgat tcgtttggcg aatatcatcc agacgatccc ttctctagcc
    10381 atgatctcca ttttaatcat tggattaggc ctgggagtca atgtcgtgat tgttactgta
    10441 tttttgtatt ccctcttacc gatcatcaag aatacgtata ccgggatgat ccaagtagac
    10501 aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg ctagacaacg actattcatg
    10561 gtagagttac cactctctgt ttctgttatc atggcaggca taagaaacgc gttagttgtt
    10621 gccatcggga taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg agatatcatt
    10681 atccgaggaa ccaatgcaac agagggcaca tcgattattt tagcaggtgc attgccaact
    10741 gcattaatgg cgatcatcac ggactggctt ttaggtattt tggaaagacg gctagatcca
    10801 gccagccgta catcacgctg atgttattgt aaaaaacaaa agcttctgcg accatttgtt
    10861 ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa taatccactc tgattcttcc
    10921 ctccttatgg gtaaagagtt gcttttctta gctatttatc cattccgtat actccaactt
    10981 catactgcca acgggttcat atctgcatcc accgactaag tttttttctt atccgctaag
    11041 gcattttgcc aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa
    11101 atactcgtct gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt
    11161 catttaagcc ttttccatca gtcctcgatt gttcgatgcc ttcagctgca tcatcaaaac
    11221 tggtgactgc cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct
    11281 catccttggc taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt
    11341 cctaaaaccg gtacacgatt cttatggact gcatccgtcg caactagact tggcgatacg
    11401 atgatccttc accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga
    11461 aaagacattg gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt
    11521 catggccgaa agcgccacga ctttaacttc ttccgtttga gtgacattca tgttcggcaa
    11581 tgtcgctttg gatacccgct ctgctaaagg aacgacactg gtattgtatt tcgtatctgt
    11641 atcttttcca aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg
    11701 attgtccatt gcttttttca acatactttt ggctgtttct cgataaacga catttccaat
    11761 tgtcttcgcc tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga
    11821 tgctccaatt ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg
    11881 tcaacattcc attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa
    11941 ttaaagaaat catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct
    12001 ttttggggag actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa
    12061 tgtgtccagg cagaataaat ttctacaact ttattttcct catcaacttt gtaaaccact
    12121 cgatgctgtc tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca
    12181 aaagactgcg tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt
    12241 ttctttaaat tcgtttgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg
    12301 actgtatagt tactcatcaa agagcttccc aatcaatatc atcgatattc gtaaagctac
    12361 tgtcgtcttt ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg
    12421 tttcttgaat cgctctccag tcttccagcc caatcaatac agcattgttt ccactacgat
    12481 cactaataat ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat
    12541 ttttacgagc agtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg
    12601 atataattat agcgtacttg attaagtacg tcaaaaaaat acaataaagc acttaaattc
    12661 agcaaacttt tcctcttttt tacatagaga aaaagacccc tttttaggga ggatctttgt
    12721 ccacgctcac ttcttactcc tttacgacta gcggtagcca aacgtaaagt aattggtaac
    12781 aacgtatcat ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca acaacaccaa
    12841 tacacgaagg ttcctcgttg cagtgtgact tgattgatat cttgaataac ctgaggatac
    12901 gcacgaaaga tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc ttctactgcc
    12961 tcaaaaatga ttgcctcttt cgtctcctgt cattcatgtt cactatcgat ttgatacttg
    13021 cttagaccgt tgggtttgtg aatcaccaat taaaaatggc actgagttat ggtaacaaaa
    13081 aatggtcact tgacgaggct gagattccta cttatgaaga cacatggcac ttaagagtcg
    13141 aggtaaagga agaagctgaa aatataggag gctgtacatt atttggcaaa ggattcggaa
    13201 attatgatca agacatcaaa atcaaaatgt tttacagcta aataggaaga ataataacat
    13261 ttcatacgct gttcccatct ttaatggctt atagagggca cctttagctg agaataagac
    13321 aaaacagacc ctctagcttc aattaagagc tagagggtct gtttttgatt ttaaaacttg
    13381 gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg ccaagacaat
    13441 cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg tctgtttggt
    13501 acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt tttacttacg
    13561 atatctacat aacggataat tggattttca atcgagcgat aagaagagtg gacgattgaa
    13621 aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc tttttcgaca
    13681 tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg tgcgtaattc
    13741 aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt attcccgtca
    13801 taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt tttataatga
    13861 tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa aggccaaatg
    13921 tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa cgcacccgta
    13981 atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt gacgaccatc
    14041 cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg aatcaagcgt
    14101 tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag tgtgataatc
    14161 ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat gaatttatct
    14221 tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc agcaactgct
    14281 aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc aaagacagct
    14341 ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata ccaatagttg
    14401 ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt caatgtagca
    14461 gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat cgcctccacc
    14521 ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat agaaacaccc
    14581 ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt cacgatatta
    14641 tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac cggaaataat
    14701 aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag caaaacgatt
    14761 gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt gatcgcttca
    14821 gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa agagccaccg
    14881 gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc tcccccgcca
    14941 tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa taaaataata
    15001 acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc gactaaaacg
    15061 acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga caataatgcc
    15121 aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa aggcttacct
    15181 actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat tttacttcaa
    15241 gttttatagt cgaaaattta tagtaaacaa gatgttatag tacgctcact gaccttaaaa
    15301 gtcaataaac tttaagaaaa taattggaaa aaaacagaaa aatatcaagc gctttttcaa
    15361 acgctttaat acagcaaaaa tctaacattt ttttatgggc tttttctttg aattctataa
    15421 taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat cttaactttt
    15481 ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag catagcgcag
    15541 ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa taagcccaac
    15601 caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg atgcagagca
    15661 gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt attagctcaa
    15721 aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact caggtcaaaa
    15781 cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc cgcttctttg
    15841 aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat tccaaagaca
    15901 acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata aattcctcct
    15961 gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt gatcatttgg
    16021 aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat cgacagtact
    16081 aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa gacatagacg
    16141 atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac cggattttgg
    16201 acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg cgcaatcgcc
    16261 attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa attgtttcgg
    16321 tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac agcgccgacc
    16381 cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc gattggataa
    16441 agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt tgttgcgtct
    16501 agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg
    16561 tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat caagtccgca
    16621 gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt caatgtaccg
    16681 gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc caatcctaat
    16741 ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt gatgccattg
    16801 acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc ttgtccaatt
    16861 gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac agttgctttc
    16921 gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca
    16981 gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact gatttgacct
    17041 gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat ttgatctagc
    17101 aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc agataggcgg
    17161 ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat tggctgtaat
    17221 ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt atcgattgca
    17281 ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat gtttttcttt
    17341 tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat taacgaacct
    17401 gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt tgtgatgctt
    17461 ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag atcacttgcc
    17521 tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat gatcgtcaag
    17581 ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc aaaatcgccg
    17641 tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc ttgcaaatca
    17701 aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc aacgactttt
    17761 tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt attcagtttt
    17821 tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta gccgtcgatc
    17881 gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat acttactagg
    17941 ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga actggctgtt
    18001 ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc ggtgattttc
    18061 ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt cttgatatca
    18121 ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat acctgcgtaa
    18181 tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa ttgccatcct
    18241 aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac ttctttacct
    18301 tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc agtatttcca
    18361 tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat gatcatgatc
    18421 gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc aagcaaatat
    18481 aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt ttcagctgaa
    18541 ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa gtcgaatttt
    18601 tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa taattaagga
    18661 ttacagcctt tactttatca tgaaataaaa gggaaagata attttatgcc aaagttttta
    18721 tctattatac gtatacgcaa aaaagctaaa acattttttg tctttgcccc tacaaaaatg
    18781 tttcagcttt tttcgttttt tattcctgta tttcttattt ttcgaatgac aggatgattt
    18841 tcccagcagg aggatgtcct tcaactagct gatgtgcttg gatgacatgc ttaagtgaag
    18901 cagacagatt cattgaaata aaaacatgaa aggctccttt ttggtaggct ttgattatgc
    18961 cagcaaaggc ttctgcatct agatattcct ttcttggaac aaacgattca taaaagcctt
    19021 cctttttttg ccgcaaatct agatctggga ggtcatttaa tgcaacatac ctgccgcctg
    19081 gtttcatgat ctggatgccg gtttctcctt tgatacttcc tttcgtcgca tcgattacaa
    19141 ggtctgcctg atcagcaaat tgcagacctg gatttgtttt atcataagca gcaaatgctg
    19201 aagctcctaa tttttttacc ttttcctcat ttttactgga tgcactggtt aagattcgga
    19261 ttcccttttc atggagaagc tggatcagac ttgacccgac cgcacctgat gctcctagta
    19321 tcattacagt atctgtcggc tggatttccg tcaaatggtt gattagatta taagccgtaa
    19381 ttcctggcgt gaccattccg gctgcttctt cccaactcat tttatctgga atttttgcta
    19441 cttttgctga tggcaacacg acttcttctc cgtaagtccc gccgactgca tggacagcta
    19501 ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc
    19561 catcattacc tggtacataa gggaatttca atgttcgtat ctctttcatt tctcctaaac
    19621 gcaaggcaac atcataagga ttcacactga atgcttttat ttctacacga acatgtccag
    19681 aaccgacttc tctgggatga gcatcgattt cttcaaaaac atcttgtgct tctccgtaac
    19741 cgttaattgc aaatcttcgc atggtcacca tctccctttt tctttaagtt tacgtttatg
    19801 cctttatttt gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa acaaaatagt
    19861 tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat tacgatttac
    19921 gctaataaaa aataattgga ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc
    19981 aaaaaaacag caagaaacga ttgaacgcta tgctgcttta cgccaggaat taaaagcgca
    20041 aaaagattac gaaggcttgc gtaaacttcc tttagatgct cgtccagaac gattgaaaaa
    20101 tcgtgattta atcgatggac gccctcgcgg atacatgcgt aaattcggga tgtcccgtat
    20161 aaaattccgt gaattagcac ataaaggttt gattccagga gtaaaaaaag ccagctggta
    20221 atccaagtct ggctgtaatg taaaggagga gggcgcatgg accactcctt ggatcatcaa
    20281 aaaagatacg ttcgcttctt ctagcagaaa tgctggtact agaagcagac gtatcttttt
    20341 ttacgcacag ctggtccttt tttatatgta aaggctgcat ggttacttcg ctatatttat
    20401 ttagtaacag gagtcagtgc ttcgataccc acactaattt tcaatgctcg gtcaacttct
    20461 gccataatgg gtcgatccaa atggcagacc ttttctttca atcttgattt atcgatcgtt
    20521 ctgatctgct ccaacaggat aacggagtct ctttctattc ctgttccagt agctttgatc
    20581 ccaatgtgag tcggaagctt aggtttagcc atttttgccg ttatggccgc aacaatgatc
    20641 gtcgggctaa aatggttacc tagattattt tgaataacca aaactgggcg tgttccgcct
    20701 tgctccgatc ccacgactgg agaaagatca gcaaaataaa tatctcctct ttttaccata
    20761 ggatcctcct tattcttgat attctcttgg tatccgctgt cctaatccgc aagccacttc
    20821 atagtggatc gtacctaatt gatctgctat atcctgcata gtgatttctt tgtctgcatt
    20881 tttgccgata agcgtcactt ttgttccgac aggaaattct tgaggcaatc ggatcatcag
    20941 ttgatccata catacacgcc caatcgtttc acagtagttc ccttctacca atagagaaaa
    21001 accctgcatc tttcgtggcc agccatctgc ataaccgatc ggtatcgttc cgatccactc
    21061 tgcttcagga gtgatatatg tttctccata gccgattcct tcacctgctg ggagtttttt
    21121 gacttgaatc aattcagaaa ccagctcaag tgctggctgc aatggataaa cttccggtag
    21181 tgcgtgccca gagggattaa gtccgtacat tgctactcca taacgaatca tattgccgat
    21241 tgtttcatca tgccataaag ccgttgcact attgctgaca tgtacatatc gaggaagttc
    21301 gggaagtttt ttcagcactt ctataaagcg ttctttttgc agattccaat atgtatcatc
    21361 tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc cattctaata cacgactttc
    21421 tttgatgaat gcagctgcct cttttacttc ttcaggtgta caaaatccga tacgacccat
    21481 cccagtgtct gcttttagat gaatcgaaag aggcgtctct agcttacttt tctctaaaac
    21541 agcagctgct gcttgcaacc attcccgtgt acctgcagtt actgataaat catgagcaac
    21601 agctacagtc agtgcgtcgt aaggaaccat attcaagata aggatgggct gagtaaatcc
    21661 agcttctctc aactcgactc cttcatctaa atttgaaaca caaaagcccg aagctccacc
    21721 tgcaactgct gcctcagctg tttcaatagc tccatgaccg tatccgttcg cttttactac
    21781 tgcaaacaat tcttttcctt gcggaagtct tgcaacttca tttgctacat tttcagttat
    21841 agctttttta tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc
    21901 ttctttctag tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat gagtaatact
    21961 gacccataca ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat tttcattttt
    22021 taaaatctcg acatcttgaa aggtaacttt tccgatccca gttccccaag ctttagagaa
    22081 agcctctttg caagcgtaac gcccgcctaa aaattctact tggcgatgaa aaggcaagga
    22141 ctggaatagt ttcatttcat cagaggtcaa gattcgtgca atgaattttg gtttttcttc
    22201 gatcagccga gtgatcctag gaagttcgac tgcatcgatc ccaattcctt ttatcatatg
    22261 cttttctctt ttcaataaat ttagaacact ctcattctac caaattataa ccattttgcc
    22321 acaaataagt ccacaaataa caaaaatgaa agtttctctt aaaaaagaga aactttcatt
    22381 ttatgtttaa tcgttatttg aacggattac aaatccgcgt tttttatcat tgtgacgttt
    22441 accgccatct ttttggctgt tattattttt gctataaccg ccttttgatt tgtttccacg
    22501 ataattaccg ccgtcacgac gattacggtt gtttccgcca ccacgtttgc catttttatt
    22561 gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg atcttcactg gcactgcatc
    22621 agctggatct tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg cagaatattc
    22681 ttctaataat ttatctgctg tttgcaggta tttatctaga ccattttctg caagttttgt
    22741 ttcgatttgt tcaactgcag cacctaattg acctttgaat gcttcttttt cggttggtgg
    22801 acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt agtccatttc
    22861 atttggtgta acgaaagtca cagacatacc gccttttccg gcacggcctg tacgtccgat
    22921 acggtgaacg tagctttctg gatcttgagg gatatcatag ttgtacacat gagtgacacc
    22981 tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca aggatatcta aatgaccgct
    23041 cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac aaatcgccat ggattccttc
    23101 tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc gttttgtacg
    23161 tccgaagacg attgttaatt caggtgtttg aacatcaaac aagcgagtca tgatatcgaa
    23221 tttttcgtat tcttttgcgc ggacatagta ttgatcgatc aagtcagctg tcatttcttt
    23281 cgctttgatc ttcacatgat gtggattttt catgaattta acgccaatat tcttgatagc
    23341 tggcggcatc gttgctgaga aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat
    23401 tttttcgata tcttccaaga agcccatatt caacatttca tctgcttcat ccaaaactaa
    23461 tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca tacgtcctgg
    23521 tgtacccaca acgatatgtg gacgatcttt caagccacgg atctgacggc caatatctgc
    23581 tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc cctaaacgat ataattcttc
    23641 ttgtgtttgg atagctaatt cacgtgttgg tgcgataact aaaccttgta attcgtgacg
    23701 atctggatcg attttttcta acattggtaa tccaaatgca gctgttttcc ctgtacctgt
    23761 ctgtgcttgt ccaataacgt cttttccagc taatgctagt ggaatcgttt ctgcttggat
    23821 aggcgttgct tcttcaaaac cagcgcgttc gactgatttc aacaattctg gtgataattc
    23881 tagttcttta aatttcaaat gaatcctcct atatggttgt taactgagga tgtagctttt
    23941 ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca gtggccacgc
    24001 acgtttttat ccattcttag cccctgtggc accaccgaaa aatcactttg cgaagtttag
    24061 gcattagaaa agccaaacgg gtcgcgttca gctttatcat tattatgaca tctggcttca
    24121 acaagttgtt tctatggcaa aaccagtcca atttctcttc atctttcggc aataggttat
    24181 tctaacatag tttttcgttt tctgcaagtt tacagatttt tatttcattt gctgaagttg
    24241 ttctaccact tcaaataagc ccatgctgtt gctgcctttt agcataatac tatctgttgc
    24301 ttcaagattt tgtttgatag catcaatcaa tttattcttt tctttttcaa aatagaacac
    24361 aggtagttgg gggtattttt ggttcaattc ttcttttagt gcatgcattt gttccccata
    24421 aaggaaaatc atggcgaatt tctcaggatc gatatgttca ctcatttgcg catgcatatt
    24481 taaagactct ttccctaatt ccagcatatc tgctaacaca gcgattctgc gtccttttgt
    24541 tggcagattt ttaaaagtat ctaataccag tcccatggct gttggatttg cattgtagac
    24601 atcgctcaaa atagctgcac catttcctgc cgttagccat tcggttctgt tttgagtgat
    24661 agaaacatgg gcgagccctt ggcgtatctc aggtatggac agtccaaaga agcgcccgat
    24721 cgtatacgca atcaaagcat tcgttacatt ataacttcca ggcagcggga tcgtaaatgt
    24781 ttctccttct actacaaaat cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac
    24841 atcgccattt ccaataccga atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt
    24901 cagcaacggt tcatctgcgg gcacaacgag taatccgtct gatacaagtc cgtcagtgat
    24961 ttccattttg gctttagcga ttccttctcg cgacccaaga ttttctatgt gtgcttcacc
    25021 gatcattgtg attgctgcag cttttggttg agccataagt gaaagttccg tgatttcatt
    25081 ggcgtgatcc atgcccattt ccaaaacgat cttttctgta tcttggggca tgtgcaaaat
    25141 cgtgtaagga agaccaatat tattgttgta atttccttgc gtcttatatg ttttgtattt
    25201 ttcagacaag acagacgcgg ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat
    25261 tgcaacgact tctgcgccta ctttttccaa gtaataggtt gccaattgct gcatggcttt
    25321 caaaggatct gctacaggaa taactggaaa attttttgga gcttgttcaa caggcaagct
    25381 ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg ataaattcat gtccatctcg
    25441 cgtaccagca agaggaacga ataaacttcc ttcggttatc ttccggctgt caaattcgac
    25501 attggtaatc tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa tttcttgtat
    25561 tgttagattc attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg
    25621 gcgggttcgc atgactctca cttccaagta aatttctcta ttcgtattta gaagctagaa
    25681 tgaacgcggt tacgcaatca ctccagcctc ttctttattt ctctttattc atttttttca
    25741 aaaaaagatt gacgctgatg gtatcgattc attcctaact ggatcagttc ttcaatcaaa
    25801 tcaccgtatt tcaagcccat attttcccat aagagtgggt acatactgaa ctccgtaaat
    25861 cctggcatag agtttaattc attcaggaat aattcatttt tatttgtcaa aaagaaatcg
    25921 caccggctca atccgcttcc acctaacatc gtgtaagcta acttcgcgta ctcttgcgct
    25981 ttttgataaa cttcttctgg cacttcggct ggaatctgca tttcgatttt attattgata
    26041 tattttgctt cataatcata gaatgctacg tcttttacga cttcaccagg caaagtcgtc
    26101 cgaacgtctt catttcctaa tacagcaact tcgatttcgc gcgcttcaat tccttgttca
    26161 acgattgctc gagaatcata ctgataggct gttgctaaag cattttgcag ctcttctcgg
    26221 ttttctgctt ttgtaatgcc gacactagaa cccatattcg ccggtttgac aaacatcgga
    26281 taaagcaaag aaccttcaca ttgatcaaat acttttttag gattttcttt ccattgattc
    26341 ttaagtactg gtacataagg aacttgcggc acaccagcag cttgtaaaat atacttggtc
    26401 atgattttat ccatggcaca tgcactggtc aatacgcctg cgccgacata aggcatattc
    26461 aatgtctcta agaagccttg gatcgttcca tcttccccgt ttggcccatg taaaactgga
    26521 aaaacgatgg ctccttcttc tttgatttcg cccggattga tcacttttcc tgtaaagcct
    26581 tcctctgtct gtccacttgg gtcccatgaa agatgcaaga catctttgct ggcaggtttt
    26641 tctgttagta atggaccttt gacccattgt ccttcttttg taataaatac gagttgaact
    26701 tggtaataat tataataaat ggcatttaaa actgaaaatg cggaaagaat ggacacttca
    26761 tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc ctcctgtatt
    26821 ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa acaaaaataa acgaataact
    26881 tatttataaa aaaacgagcc agcttctaaa agaacgagcg tcatttattt tttgtcagct
    26941 agattatttg tcccgttttt tataaaaagt tatgataaag aaaaggaggg atgatgatgg
    27001 cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg agtgttcatc aattacaatg
    27061 tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac
    27121 taggccccac cttcatcaag ataggacaga tgctttctgt tcgagaagat ttgctttctt
    27181 ctgccttcac tcaaacattc aaaacattac aagatagtgt acctagcgac acattttcaa
    27241 cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt atcagatata ttcgatgatt
    27301 tttcaaaatc gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc aagctgaaga
    27361 acggtgacgc ggtagtcgtt aagatccaac accctaacat cgccgaagag atccgacttg
    27421 atcttcaact atttgaacga gcgatccctt tgatcaaata cattcctgaa actagcgtag
    27481 ttgatctaaa aggggtactc caagaagtaa aacgttcctt gatcaatgaa atggacttct
    27541 taaaagaaag tcaaaatggc gaacaattct atcaaaagaa caatggctgg aaagagatcc
    27601 gcagccccaa gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt
    27661 ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact tttatcactg
    27721 gaattcaaaa caagcagctg aaacaagaag ttgcaaagct tttagttgag aactttatga
    27781 aacaggtgtt tgatgatggt tttttccatg ctgatcctca tccagggaat ctgctattcc
    27841 atgtactaac aaaagaagaa cagacacaag ctagccgaaa aactgaaaca gtccatgaaa
    27901 aagagttcgg ttcctttgct tttcgagcct ccacttctgc agaagaccct gttgcgccat
    27961 ataccatcaa ctacatcgat tttggtatga tggggcatct ttctgcagga cttaggcaaa
    28021 aactcacaca agccgtttta gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg
    28081 tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac caagaactga
    28141 caagttttct tgaacagtat tatgattctc ctattgatga aatcaacttg caagaagtat
    28201 tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc
    28261 ttttgctgaa agcatttggg acattagaag gggtgatccg cgtcctcgat cctgaagttt
    28321 ccctcatgga agtcgcttcg ccttttgctc aacattattt tctcactcat ctggatgtag
    28381 aagatacatt gaaacaaagc ggattagact tattggaagg gatgaaggct gcgcctaaga
    28441 tccctcaaca gctccaccat ctattagaaa tgtggacttc gggacaagga aaagtcaatt
    28501 tggaattgaa aaagcaagac aaattattga gcaggatcga aagtatgatc aatcggcttg
    28561 ttttcggtat gattttagct gcattgatcg tagggtcctc cctgcttgtc caagcagcac
    28621 ctgtagaaaa cgcagaggtc gtttccttat tagggatatt tacgtatgct attgccgcat
    28681 tcgtcattat ttttttggca atcgatgcat tgatccaaat gtataagaag aggaaaaagt
    28741 agaaaaaggt tgtgacagaa agaatcagct tcaagaaata agaaggggtt ttacataaat
    28801 tgtttctcaa atttttgtga aaattcggct tatttttcga agaggctact tctgaaacac
    28861 cgagaataaa aaatgtgaaa aagctgttct gcacccagta ataagaacaa tcaaaaaggg
    28921 aacatggaac acatttatcc tgtttccctg ttcccttttt gatgtattcg agcataaata
    28981 aaataccttc gttacctact atcttctgta cttggtaaac cttagtcaat tgcctttttt
    29041 aacgcttgac gaaaagacat atgatctttc tccgtaaagg gttttggacc ctttgtcctt
    29101 atgccagctt ttcttaactt tccactatga taacgttgtt ctagaagcgt gccgatattc
    29161 gcttcgtcaa attcttttcc agagtgatgg aacactcgca cacccttcgg caatacaagt
    29221 gaagctaaac cataatcttg cgtgatcaaa aagtcattct tcttaaccat accgacgatt
    29281 ttataatccg cactatcagc tccgcggtct acgtagacga tttggacatg attagcgtat
    29341 tctttggtcg tataatggtc cacactggtc acgataacca cgtcaagacc ataggattct
    29401 cctaatttta tgacatcttc ttttactgga gaaccatctc catcaatgaa tagcctcatt
    29461 ctttttcgcc ttcttccata aatgataaac agttactaat ccgctcagta aaagaccaac
    29521 attgatccat tttgccaaga taagcagttg tacactttct atcggcactg attcatggtt
    29581 cagcgtgatc aataatagat ggattggctc tcccatttct tttgctaaaa caaacaaaag
    29641 cgtcatcaat aaaaactggt ggatcacact ccctttattt tgagtagtat acttgacaac
    29701 gatccggcga tccattggaa tagctgtatc agtttgataa tattccttat ttagccagct
    29761 gcctgttact acgtgccaga aactaagcag aaagacacct atcgcaaaac catttggaat
    29821 caagaggacc aataaagaag aactcagtcc aagcaattgc atagccaatg gagaccatgt
    29881 taaacgtttc aaaatccatg gtccgactat ttttgctgca atcaagcctg ctgcataagg
    29941 caaatacata aaaatcgaaa gtttttctaa tccgtacagt atgccaacat aaaaactgct
    30001 aaataaaaaa aggaaattcc ctagcattcc atttagaaaa gtaaacagat ttagccagct
    30061 tggcaacttc cattccttct tctggtgtcc taaaacaaac acgaacaaga tgaacagcag
    30121 caagaaacca taaatagcaa gatcaaaaag cctttcatcg aataacaatc gggcatttct
    30181 caaaagaaac aataagccaa agaatatcaa aaataaaaca agctctcgcg tagctaccag
    30241 atttcttttg atgtctttga aatccagttc gtaacgcgga taatgactaa ccgtatggta
    30301 ggccatgacg taaaaaagtg tataaatagt aaagcttgct gcaatctgag tcggtgcagg
    30361 caactcaatc gcttgataca ggagaaatag ccagatagct gcaaataagt actttttccc
    30421 atccatattg ataaaacctt gcgtcttttc gtgaaaattg accgtggtat tagcaggtac
    30481 aagccatgct gcactcagcc ctagcaaaac acttgaaaaa taaaataaag gaaaataaaa
    30541 tacgcctaat attccaagca atgaaccagc accaccaatc agcagagcga tcatcagcaa
    30601 cgtgtaacta tctagaccaa atcgtagact gttgactaaa aatagtccag tcatccgaaa
    30661 ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg ttagtcccaa
    30721 aaataaaaga taaggaataa aaataaccgt gttacttaag ataaagggcg ttgtttgtgt
    30781 aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca atggctcctt aaacagtctt
    30841 ataactaatc tgctcttcga ttttagcatt aaaggggaaa taaaccaatt ttacagaata
    30901 tattcttgta aaaaaaaata cagaaagaag cataagcttc tttctgcatt tttacggtac
    30961 ttgattactc aagcagacaa gcccttctcc acaaaagtct tgcccgacac ccctgcatcc
    31021 ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt aggagctctg
    31081 taaaccagag tccttctcga cccgtcgcat aaatttactt taacacagtt cctatgttta
    31141 tgcgagagct tttttcattt tataatcgac catttgaaat ccttgaccga tatctgaaat
    31201 aagttcttct gtaatctcaa atccttcatg cttataaacc tcgattgctt gatgattccc
    31261 ctgattcact cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt cttcatacca
    31321 gcgaaaaact gaagaagtca atccttgacc acgtacttta tttagcaaat ataatttact
    31381 taaataaatt tttccattta tctcttcata agcagtgtaa ccgacagtct tttcttcgct
    31441 ttttaataaa aagtaggaca caccttctgt cagttctttt tgaatctgtt tttttgactg
    31501 ataagttttc agcatgtaat ccacttgttt ttgaccaatg atcggcacaa aaacttccgg
    31561 ccatacttct tgtatgactg ccatcagctc atccagttca ttttctgtta cttttatttt
    31621 ttccatacac ccacgtcctt ttttacatta taaatgacct tgcgcttgat ggaataaaaa
    31681 gagcatgatc atgctataca cacccaccga ccacggtttc aagaaaatca caatctgcat
    31741 aaattcccta aaagataatt tcgtcaaacc agctactaga cagatcaagt cgtctggcgc
    31801 aaagggaagc agcaaggtaa caactagtac ttttttaata tctttcgttt ttcttgtcag
    31861 caattcatca aatttctgat acttttttgg cgataaaata agctggacaa aggctacgcc
    31921 atagtaacgg acaagcaaaa atccgattgc ttccccaatc actaggccaa tacaactata
    31981 taaaagccca aatccattgc caaacatcag catgcccgct actgaggaga tccctcccgg
    32041 caaaatagga atgatcactt ggatcacttg caacagaaca aatatcatca ctgcatactt
    32101 gccaaattgc tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata
    32161 agcataaaga atcaacacaa agaaaaaaaa cagaccaata atgggcatga tctgaagaca
    32221 tttttgtaaa actgatcctt ttttagacat ctatattccc cacatctttt ttcatttatt
    32281 aaaactgcct cctt
//
LOCUS       DAYYOA010000019        32174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000019 DAYYOA010000000
VERSION     DAYYOA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.019"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACHBKA_000824"
     CDS             complement(407..766)
                     /locus_tag="ACHBKA_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM9444535.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACHBKA_000825"
     CDS             complement(796..1137)
                     /locus_tag="ACHBKA_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444536.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACHBKA_000826"
     CDS             complement(1134..1808)
                     /locus_tag="ACHBKA_000826"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9444537.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHBKA_000827"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHBKA_000827"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM9444538.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACHBKA_000828"
     CDS             complement(4322..5512)
                     /locus_tag="ACHBKA_000828"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9444539.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACHBKA_000829"
     CDS             complement(5533..6060)
                     /locus_tag="ACHBKA_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM9444540.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACHBKA_000830"
     CDS             complement(6107..8896)
                     /locus_tag="ACHBKA_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM9444541.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACHBKA_000831"
     CDS             complement(9043..9243)
                     /locus_tag="ACHBKA_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9444542.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACHBKA_000832"
     CDS             complement(9696..10016)
                     /locus_tag="ACHBKA_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444543.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACHBKA_000833"
     CDS             complement(10519..11637)
                     /locus_tag="ACHBKA_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444544.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACHBKA_000834"
     CDS             11870..11992
                     /locus_tag="ACHBKA_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444545.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACHBKA_000835"
     CDS             12108..13415
                     /locus_tag="ACHBKA_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM9444546.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACHBKA_000836"
     CDS             13536..14735
                     /locus_tag="ACHBKA_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9444547.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACHBKA_000837"
     CDS             complement(14762..16030)
                     /locus_tag="ACHBKA_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM9444548.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACHBKA_000838"
     CDS             complement(16200..16841)
                     /locus_tag="ACHBKA_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM9444549.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACHBKA_000839"
     CDS             complement(17250..17753)
                     /locus_tag="ACHBKA_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9444550.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACHBKA_000840"
     CDS             complement(17876..18640)
                     /locus_tag="ACHBKA_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM9444551.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACHBKA_000841"
     CDS             18748..19023
                     /locus_tag="ACHBKA_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM9444552.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ACHBKA_000842"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ACHBKA_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM9444553.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ACHBKA_000843"
     CDS             complement(20579..20980)
                     /locus_tag="ACHBKA_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9444554.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ACHBKA_000844"
     CDS             complement(20994..21803)
                     /locus_tag="ACHBKA_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9444555.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ACHBKA_000845"
     CDS             complement(21790..22680)
                     /locus_tag="ACHBKA_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9444556.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ACHBKA_000846"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="ACHBKA_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="ACHBKA_000847"
     CDS             complement(23348..24289)
                     /locus_tag="ACHBKA_000847"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM9444557.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHBKA_000848"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHBKA_000848"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9444558.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ACHBKA_000849"
     CDS             complement(25274..26470)
                     /locus_tag="ACHBKA_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9444559.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ACHBKA_000850"
     CDS             complement(26488..27216)
                     /locus_tag="ACHBKA_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9444560.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACHBKA_000851"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACHBKA_000851"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9444561.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ACHBKA_000852"
     CDS             complement(28628..29086)
                     /locus_tag="ACHBKA_000852"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM9444562.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ACHBKA_000853"
     CDS             complement(29083..30546)
                     /locus_tag="ACHBKA_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9444563.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACHBKA_000854"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACHBKA_000854"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9444564.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32174
                     /locus_tag="ACHBKA_000855"
     CDS             31602..>32174
                     /locus_tag="ACHBKA_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9444565.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFF"
BASE COUNT         9382 a         6921 c         5511 g        10360 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt tttt
//
LOCUS       DAYYOA010000020        30674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000020 DAYYOA010000000
VERSION     DAYYOA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.020"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..312)
                     /locus_tag="ACHBKA_000856"
     CDS             complement(1..312)
                     /locus_tag="ACHBKA_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9444566.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(314..511)
                     /locus_tag="ACHBKA_000857"
     CDS             complement(314..511)
                     /locus_tag="ACHBKA_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9444567.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(905..2632)
                     /locus_tag="ACHBKA_000858"
     CDS             complement(905..2632)
                     /locus_tag="ACHBKA_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM9444568.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2625..3818)
                     /locus_tag="ACHBKA_000859"
     CDS             complement(2625..3818)
                     /locus_tag="ACHBKA_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9444569.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4005..4649)
                     /locus_tag="ACHBKA_000860"
     CDS             complement(4005..4649)
                     /locus_tag="ACHBKA_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9444570.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4743..4877)
                     /locus_tag="ACHBKA_000861"
     CDS             complement(4743..4877)
                     /locus_tag="ACHBKA_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM9444571.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4879..7011)
                     /gene="feoB"
                     /locus_tag="ACHBKA_000862"
     CDS             complement(4879..7011)
                     /gene="feoB"
                     /locus_tag="ACHBKA_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM9444572.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7008..7481)
                     /locus_tag="ACHBKA_000863"
     CDS             complement(7008..7481)
                     /locus_tag="ACHBKA_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM9444573.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7882..8106
                     /gene="nrdH"
                     /locus_tag="ACHBKA_000864"
     CDS             7882..8106
                     /gene="nrdH"
                     /locus_tag="ACHBKA_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM9444574.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8265..10424
                     /gene="nrdE"
                     /locus_tag="ACHBKA_000865"
     CDS             8265..10424
                     /gene="nrdE"
                     /locus_tag="ACHBKA_000865"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM9444575.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10474..11439
                     /gene="nrdF"
                     /locus_tag="ACHBKA_000866"
     CDS             10474..11439
                     /gene="nrdF"
                     /locus_tag="ACHBKA_000866"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM9444576.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11528..12667)
                     /locus_tag="ACHBKA_000867"
     CDS             complement(11528..12667)
                     /locus_tag="ACHBKA_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9444577.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12976..13689)
                     /locus_tag="ACHBKA_000868"
     CDS             complement(12976..13689)
                     /locus_tag="ACHBKA_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM9444578.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13943..14644)
                     /gene="nagB"
                     /locus_tag="ACHBKA_000869"
     CDS             complement(13943..14644)
                     /gene="nagB"
                     /locus_tag="ACHBKA_000869"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM9444579.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14878..16410
                     /locus_tag="ACHBKA_000870"
     CDS             14878..16410
                     /locus_tag="ACHBKA_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9444580.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16601..17803)
                     /locus_tag="ACHBKA_000871"
     CDS             complement(16601..17803)
                     /locus_tag="ACHBKA_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9444581.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17885..18574)
                     /locus_tag="ACHBKA_000872"
     CDS             complement(17885..18574)
                     /locus_tag="ACHBKA_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9444582.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18680..19048)
                     /gene="rplL"
                     /locus_tag="ACHBKA_000873"
     CDS             complement(18680..19048)
                     /gene="rplL"
                     /locus_tag="ACHBKA_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM9444583.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19101..19604)
                     /gene="rplJ"
                     /locus_tag="ACHBKA_000874"
     CDS             complement(19101..19604)
                     /gene="rplJ"
                     /locus_tag="ACHBKA_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM9444584.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19808..19935)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20014..20703)
                     /gene="rplA"
                     /locus_tag="ACHBKA_000875"
     CDS             complement(20014..20703)
                     /gene="rplA"
                     /locus_tag="ACHBKA_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM9444585.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20807..21229)
                     /gene="rplK"
                     /locus_tag="ACHBKA_000876"
     CDS             complement(20807..21229)
                     /gene="rplK"
                     /locus_tag="ACHBKA_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM9444586.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21572..22240
                     /gene="sdaAB"
                     /locus_tag="ACHBKA_000877"
     CDS             21572..22240
                     /gene="sdaAB"
                     /locus_tag="ACHBKA_000877"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM9444587.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22253..23125
                     /gene="sdaAA"
                     /locus_tag="ACHBKA_000878"
     CDS             22253..23125
                     /gene="sdaAA"
                     /locus_tag="ACHBKA_000878"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM9444588.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23216..24316)
                     /locus_tag="ACHBKA_000879"
                     /pseudo
     CDS             complement(23216..24316)
                     /locus_tag="ACHBKA_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24408..25295)
                     /locus_tag="ACHBKA_000880"
     CDS             complement(24408..25295)
                     /locus_tag="ACHBKA_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9444589.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25372..25917)
                     /gene="nusG"
                     /locus_tag="ACHBKA_000881"
     CDS             complement(25372..25917)
                     /gene="nusG"
                     /locus_tag="ACHBKA_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM9444590.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26018..26188)
                     /gene="secE"
                     /locus_tag="ACHBKA_000882"
     CDS             complement(26018..26188)
                     /gene="secE"
                     /locus_tag="ACHBKA_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM9444591.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26212..26364)
                     /gene="rpmG"
                     /locus_tag="ACHBKA_000883"
     CDS             complement(26212..26364)
                     /gene="rpmG"
                     /locus_tag="ACHBKA_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9444592.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26526..27170
                     /gene="cbpA"
                     /locus_tag="ACHBKA_000884"
     CDS             26526..27170
                     /gene="cbpA"
                     /locus_tag="ACHBKA_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM9444593.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27226..27729)
                     /locus_tag="ACHBKA_000885"
     CDS             complement(27226..27729)
                     /locus_tag="ACHBKA_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9444594.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27742..27788)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28326..29291
                     /locus_tag="ACHBKA_000886"
     CDS             28326..29291
                     /locus_tag="ACHBKA_000886"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9444595.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29390..30562)
                     /locus_tag="ACHBKA_000887"
     CDS             complement(29390..30562)
                     /locus_tag="ACHBKA_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444596.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30549..>30674)
                     /locus_tag="ACHBKA_000888"
     CDS             complement(30549..>30674)
                     /locus_tag="ACHBKA_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444597.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9362 a         6295 c         5419 g         9598 t
ORIGIN      
        1 ttaaaattga gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct
       61 tccaatttca ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt
      121 agtataagca gtaaaatata gataattcac caatctattt attaaaggac cgggatctaa
      181 ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga
      241 atatttagga tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc
      301 attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca
      361 ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat
      421 acactccatt tccataatat tttccactat gagtggtacc cccatagata agttgtgtct
      481 ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt
      541 agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa
      601 aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa
      661 tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct
      721 acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc gtctttggac
      781 gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat
      841 ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt
      901 ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg
      961 cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg
     1021 accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc
     1081 gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt
     1141 tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt
     1201 gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt
     1261 tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag
     1321 ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat
     1381 ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta
     1441 ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag
     1501 ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta
     1561 atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg
     1621 aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag
     1681 atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca
     1741 gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct
     1801 ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt
     1861 tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc
     1921 cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat
     1981 tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac
     2041 gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc
     2101 ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg
     2161 gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg
     2221 tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca
     2281 agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga
     2341 cagtattcat caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg
     2401 tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca
     2461 agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct
     2521 gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga
     2581 tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc
     2641 acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat
     2701 tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca
     2761 cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga
     2821 cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc
     2881 tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg
     2941 tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta
     3001 agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg
     3061 gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt
     3121 ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga
     3181 acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga
     3241 gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg
     3301 ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc
     3361 aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca
     3421 cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag
     3481 ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac
     3541 ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc
     3601 aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct
     3661 ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa
     3721 atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca
     3781 aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta
     3841 aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg
     3901 tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa
     3961 taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga
     4021 tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga
     4081 acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta
     4141 tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa
     4201 cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc
     4261 ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc
     4321 tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt
     4381 acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca
     4441 cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta
     4501 gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc
     4561 actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc
     4621 gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct
     4681 catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact
     4741 gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc
     4801 ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt
     4861 aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt
     4921 cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag
     4981 atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa
     5041 actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc
     5101 attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa
     5161 ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc
     5221 tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt
     5281 ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag
     5341 cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga
     5401 aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa
     5461 gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac
     5521 cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc
     5581 agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag
     5641 aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc
     5701 aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg
     5761 atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc
     5821 aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat
     5881 gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa
     5941 tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa
     6001 gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc
     6061 tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt
     6121 ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca
     6181 acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga
     6241 cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt
     6301 ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg
     6361 ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat
     6421 tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc
     6481 tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga
     6541 ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa
     6601 aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt
     6661 gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt
     6721 agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg
     6781 ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga
     6841 atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt
     6901 tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt
     6961 gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc
     7021 tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc
     7081 actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt
     7141 cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg
     7201 acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca
     7261 gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt
     7321 tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca
     7381 acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact
     7441 tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga
     7501 aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa
     7561 taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg
     7621 tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca
     7681 tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc
     7741 agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag
     7801 tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga taataagaat
     7861 gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa
     7921 tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc
     7981 gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc
     8041 attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc
     8101 agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca
     8161 ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt
     8221 gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt
     8281 tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa
     8341 tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa
     8401 atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat caagaatata
     8461 tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt
     8521 tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat
     8581 atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct
     8641 tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa
     8701 tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc
     8761 ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc
     8821 gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat
     8881 caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg
     8941 ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc
     9001 aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag
     9061 caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg
     9121 tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat
     9181 atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg
     9241 atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa
     9301 atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag
     9361 cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt
     9421 tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacgg
     9481 atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg
     9541 gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg
     9601 atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca
     9661 tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag
     9721 actttacaaa tatttacttc atgctgttga attattggac acttgttgaa agcaacaata
     9781 tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca
     9841 cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat
     9901 tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa
     9961 tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat
    10021 atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag
    10081 agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt
    10141 attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaatggcac
    10201 aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag
    10261 gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac
    10321 attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg
    10381 cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc
    10441 gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata
    10501 aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa
    10561 ttttggctag atacacgtat cccactttca aatgacctag atgattggcg tacattgtct
    10621 caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc
    10681 caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc
    10741 gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt
    10801 agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct
    10861 tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa
    10921 aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct
    10981 ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc
    11041 cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag
    11101 ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt
    11161 tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa
    11221 gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca
    11281 ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc
    11341 acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa
    11401 gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa
    11461 tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt
    11521 tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat
    11581 gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg
    11641 catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca
    11701 ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga
    11761 tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa
    11821 atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat
    11881 acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga
    11941 atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc
    12001 tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa
    12061 tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa
    12121 atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga
    12181 tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata
    12241 aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca
    12301 attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa
    12361 ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta
    12421 cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa
    12481 caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaactg
    12541 ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc
    12601 cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg
    12661 attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt
    12721 acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt
    12781 agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc
    12841 tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa
    12901 aaatataagg aactattttt atttggctgg gcttattact tgatgtagga catctatttt
    12961 acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg
    13021 tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata
    13081 gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat
    13141 tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat
    13201 aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag
    13261 tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc
    13321 gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg
    13381 tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat
    13441 cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct
    13501 taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac
    13561 taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc
    13621 tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat
    13681 atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa
    13741 agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt
    13801 ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata
    13861 tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac
    13921 ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa
    13981 caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa
    14041 tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac
    14101 ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat
    14161 cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc
    14221 cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga
    14281 tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca
    14341 gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt
    14401 ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat
    14461 ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata
    14521 aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt
    14581 ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt
    14641 ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt
    14701 ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa
    14761 tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc
    14821 aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg
    14881 agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt
    14941 gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt
    15001 aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat
    15061 tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta
    15121 acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt
    15181 gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg
    15241 aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag
    15301 atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt
    15361 ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg
    15421 atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt
    15481 cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag
    15541 atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta
    15601 aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct
    15661 caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga
    15721 tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca
    15781 gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca
    15841 gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg
    15901 accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact
    15961 ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat
    16021 aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca
    16081 ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta
    16141 gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg
    16201 gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc
    16261 gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag
    16321 aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg
    16381 caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag
    16441 ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca attagctgac
    16501 cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt
    16561 attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat
    16621 ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc
    16681 cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac
    16741 cagccgcacc tccaataaag tacataaaca taaatacagt attatttcgg ttgcttgctg
    16801 cttcacctag cccttgtact cgtgtttgat tggcgacttg accaaattga ttcccgacgt
    16861 ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc aataaagcaa
    16921 taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca gaataagaat
    16981 ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa atcgctaata
    17041 tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa atcaacgtag
    17101 accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct tctcttaata
    17161 gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat gaaacagctg
    17221 tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta agaattccga
    17281 ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca acaataccag
    17341 ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta cccatgacct
    17401 tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt tgcggcacga
    17461 tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa ggcgcaaaaa
    17521 aagcggccaa cagagagacg gcagataacg ctgccattcg aatgatcaat tttggacgat
    17581 ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct aattgggtca
    17641 acatcgtcaa tatcccgact gcacttgtgc taacagataa actagctgct acttttgttc
    17701 caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt gataatagta
    17761 aaataagcga agttgttaaa ggtttttttt cattactctt cattgatttc ccccatttcc
    17821 tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact attcgccaca
    17881 cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa atgaagacaa
    17941 ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg ttatatccgt
    18001 gaaacagagc ttctccacca cacgcataca acacagacga agggatgact ccaatcacga
    18061 tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa ttgaccatag
    18121 aagatggaat aaccggtatc atatagccta acattacacc tatttttgga tgtttcattt
    18181 gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat tttaagcgat
    18241 gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg atgatcgcac
    18301 ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta ggaatacctg
    18361 gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa gtcccatgcg
    18421 aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat aattggatgt
    18481 ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg attcccaacg
    18541 agatcaatat caatcggatt attttctttt tcatatttcc tcctgaaaaa aatctatatt
    18601 cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat aggatctcag
    18661 tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct tcaagtttag
    18721 ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca ggagcgccat
    18781 caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact gctttgataa
    18841 ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt tgttcttcag
    18901 cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat acgccaaatt
    18961 cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct tttaactcag
    19021 caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg ttctttttta
    19081 tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc cactgctttg
    19141 acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat agatagaagt
    19201 ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga gactttacct
    19261 tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc gtccatgatt
    19321 ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc agtgaatact
    19381 tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga atttttgata
    19441 actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac ttcatcaact
    19501 gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt ttcgcttact
    19561 tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga gtttcacctc
    19621 ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta tagttacttt
    19681 gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta ctcgctttgc
    19741 tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc ttcacttcgt
    19801 tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga ctattgaata
    19861 tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg tcttcggtac
    19921 aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca agaattattt
    19981 ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt caacatggat
    20041 accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt tagctgctga
    20101 tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta atttttcatc
    20161 gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca cacggtaagt
    20221 tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa ctgtaccagt
    20281 tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac ctacagtagc
    20341 catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt ggattttttg
    20401 aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt ttgctttgtc
    20461 tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta atacaacagc
    20521 accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg caacttcaac
    20581 tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt cggcaacagg
    20641 ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc tctttttagc
    20701 cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg ttctatatct
    20761 ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat tctacagtga
    20821 tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg ttagctgcgt
    20881 ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa actttcgcaa
    20941 ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt ttcaatagaa
    21001 cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat acagaaataa
    21061 caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct tttgtgaatc
    21121 ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct ggagttgctt
    21181 tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg agacatacct
    21241 ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg ttttatctga
    21301 tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag tatagcaaat
    21361 ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa taaacgctaa
    21421 atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt tacacttgcg
    21481 ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc cttgtacaaa
    21541 gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata gaagtgtctt
    21601 tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg gagctgctag
    21661 aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag atatttatct
    21721 atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat tagttggcgg
    21781 cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat tggcatatga
    21841 agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc caaactctgt
    21901 aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt cgatcggcgg
    21961 tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca tgggtactcc
    22021 taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga ctagtatact
    22081 ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa aaggtgaaaa
    22141 tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa aaagactcaa
    22201 acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa aaatgttttt
    22261 atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag aattgatgat
    22321 tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga tcatggaaaa
    22381 aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta cctccgtcac
    22441 aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg cagacttttt
    22501 aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg aagtcaatgc
    22561 aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg ttcctggtgt
    22621 gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg aattcttatt
    22681 taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg gtgcagaagg
    22741 gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt tggtttgtgc
    22801 aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga aaaatatgat
    22861 gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca aacgtaatgc
    22921 tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta tcgaaagtgt
    22981 cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa tgccgtcgat
    23041 tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac gacttgcgga
    23101 agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta agagattagt
    23161 tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt ttttattaat
    23221 ccatcaattt cgtatttttt acttcatcga tcgtttttgt tcctgcaagt tgcatcgtga
    23281 tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc ccacctagat
    23341 tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt gccagagctt
    23401 tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca cgtttgttga
    23461 ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct agttgacgtc
    23521 caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt actgcatctt
    23581 ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg attgttttga
    23641 tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt ccttttcctt
    23701 cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt ggtaatggaa
    23761 attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa tctgccgtta
    23821 aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca ttgaatctgt
    23881 cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca tcggctgcgt
    23941 ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca gcagctactc
    24001 ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct gatggcgctt
    24061 ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg gctgagtcga
    24121 ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta ttttctttca
    24181 tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct tccatgcgct
    24241 cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt tcttcattac
    24301 ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat aaatcatact
    24361 aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta ttctttccaa
    24421 ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact atgagaggca
    24481 ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa caacgcatag
    24541 accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc atctagttga
    24601 ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg ctggtaatct
    24661 tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag agactgcgca
    24721 ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg aacttgtggc
    24781 aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc tcccatggaa
    24841 tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa aagacaagtt
    24901 ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa tacttggacc
    24961 atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc atctgttcct
    25021 actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg caacatccct
    25081 ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat agtggttttt
    25141 tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt agtatttatc
    25201 ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc tgctgcagtt
    25261 gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc catatagaaa
    25321 aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt attaatcgat
    25381 tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca tatcgatgtt
    25441 gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta atccagcaaa
    25501 tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat tttgtcgcag
    25561 gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg gtgctggttt
    25621 gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc ggacaacata
    25681 ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg ttttatgaac
    25741 gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa caacgacacg
    25801 gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg cttttacttt
    25861 gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag attccattga
    25921 aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt tttctttcgc
    25981 ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc cagccaaata
    26041 agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata gaagtttcga
    26101 tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact tgtttgattt
    26161 catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg tttatttcgt
    26221 ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt ccaaacgttc
    26281 gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa cggaacaagc
    26341 gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc agacttatac
    26401 ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact atctattgtt
    26461 ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca attggaggta
    26521 tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc gttacagaga
    26581 ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt tgtgtgccga
    26641 tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat atctatcgtc
    26701 ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa aacgctacaa
    26761 agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa gagcttcctt
    26821 atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat agtacgttgc
    26881 ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg acaattgctt
    26941 ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa cacagcagca
    27001 ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt acattgatca
    27061 cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac ttagagaaaa
    27121 agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa tcaaaaagcg
    27181 aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga cacgcttttc
    27241 aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca tgacgataaa
    27301 ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg ttggaactaa
    27361 catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata ctaaacgcat
    27421 ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa gcaatgtttg
    27481 gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat agacgatgac
    27541 cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat tcaaactttc
    27601 tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag ttaacgctag
    27661 ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc tgcttttcgt
    27721 tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg ttgatgaacc
    27781 gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca atccgtctta
    27841 tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa tcgtgggata
    27901 tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg aagtaggatc
    27961 tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg aatttgagca
    28021 acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca tgccgaacct
    28081 tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt tgtgtttttc
    28141 ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga attaattttg
    28201 taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa agcttgctaa
    28261 ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa tagcaggagg
    28321 aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc ggaaatggct
    28381 ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac tatacacttc
    28441 attatagtga atacaaagga cacgatgctg agattgctga aactttggca aaagaaacat
    28501 tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt ttgatcatcg
    28561 taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg gaagtagaat
    28621 tcccagttgc ttatattcca gcaggttctg gagatgattt tgctcgaggg gcaggccttc
    28681 caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca gaaaaagtcc
    28741 acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt aataatttcg
    28801 gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca aaaaagcgat
    28861 tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt gctttattca
    28921 ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt ttttcaaacg
    28981 catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg attgttccaa
    29041 ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc aatttcttta
    29101 aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg aaatatttcc
    29161 atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat ggtcaagaag
    29221 atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact aaacagttgt
    29281 tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt ttattcggaa
    29341 aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt taattctttg
    29401 cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg atcattgaaa
    29461 acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct gcaaatgttt
    29521 gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa tatgttggtg
    29581 tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca aacaccccac
    29641 cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt tctgagtttt
    29701 ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac aagaaagtat
    29761 ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac agaaccatta
    29821 gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag gttactttgc
    29881 gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc tctttgcgat
    29941 taaatgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac ccataagaca
    30001 agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat cccgcactta
    30061 gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct gcatcgattg
    30121 ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact tgctgtgtaa
    30181 caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcggc atctttccta
    30241 cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca cgattaaaat
    30301 aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact gtttccttgt
    30361 cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta atcaaggcga
    30421 ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa aagaatgtgg
    30481 caacaacagt gataattaca cctatcagta aattcgattt atttgcttta agcaatgtcc
    30541 aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt tcctaacgca
    30601 aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc caaatcagtg
    30661 tagaaataaa gttt
//
LOCUS       DAYYOA010000021        29412 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000021 DAYYOA010000000
VERSION     DAYYOA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.021"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..507)
                     /locus_tag="ACHBKA_000889"
     CDS             complement(<1..507)
                     /locus_tag="ACHBKA_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM9444598.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLD"
     gene            complement(511..1371)
                     /gene="rlmB"
                     /locus_tag="ACHBKA_000890"
     CDS             complement(511..1371)
                     /gene="rlmB"
                     /locus_tag="ACHBKA_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM9444599.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(1364..1759)
                     /locus_tag="ACHBKA_000891"
     CDS             complement(1364..1759)
                     /locus_tag="ACHBKA_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM9444600.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(1756..3168)
                     /gene="cysS"
                     /locus_tag="ACHBKA_000892"
     CDS             complement(1756..3168)
                     /gene="cysS"
                     /locus_tag="ACHBKA_000892"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM9444601.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3165..3701)
                     /gene="epsC"
                     /locus_tag="ACHBKA_000893"
     CDS             complement(3165..3701)
                     /gene="epsC"
                     /locus_tag="ACHBKA_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM9444602.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(3787..4026)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4023..5483)
                     /gene="gltX"
                     /locus_tag="ACHBKA_000894"
     CDS             complement(4023..5483)
                     /gene="gltX"
                     /locus_tag="ACHBKA_000894"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM9444603.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(5653..6810)
                     /locus_tag="ACHBKA_000895"
     CDS             complement(5653..6810)
                     /locus_tag="ACHBKA_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM9444604.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(6844..8214)
                     /gene="radA"
                     /locus_tag="ACHBKA_000896"
     CDS             complement(6844..8214)
                     /gene="radA"
                     /locus_tag="ACHBKA_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM9444605.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8277..8804)
                     /locus_tag="ACHBKA_000897"
     CDS             complement(8277..8804)
                     /locus_tag="ACHBKA_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM9444606.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9034..9840
                     /gene="proB"
                     /locus_tag="ACHBKA_000898"
     CDS             9034..9840
                     /gene="proB"
                     /locus_tag="ACHBKA_000898"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM9444607.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            9837..11084
                     /locus_tag="ACHBKA_000899"
     CDS             9837..11084
                     /locus_tag="ACHBKA_000899"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM9444608.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11160..11504
                     /locus_tag="ACHBKA_000900"
     CDS             11160..11504
                     /locus_tag="ACHBKA_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444609.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(11621..11947)
                     /locus_tag="ACHBKA_000901"
     CDS             complement(11621..11947)
                     /locus_tag="ACHBKA_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444610.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(12562..13764)
                     /locus_tag="ACHBKA_000902"
     CDS             complement(12562..13764)
                     /locus_tag="ACHBKA_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9444611.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            13881..14396
                     /locus_tag="ACHBKA_000903"
     CDS             13881..14396
                     /locus_tag="ACHBKA_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9444612.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(14595..15041)
                     /locus_tag="ACHBKA_000904"
     CDS             complement(14595..15041)
                     /locus_tag="ACHBKA_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9444613.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15088..16566)
                     /gene="msr(C)"
                     /locus_tag="ACHBKA_000905"
     CDS             complement(15088..16566)
                     /gene="msr(C)"
                     /locus_tag="ACHBKA_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9444614.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(16920..17549)
                     /locus_tag="ACHBKA_000906"
     CDS             complement(16920..17549)
                     /locus_tag="ACHBKA_000906"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9444615.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(17533..17997)
                     /locus_tag="ACHBKA_000907"
     CDS             complement(17533..17997)
                     /locus_tag="ACHBKA_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9444616.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(18484..19752)
                     /locus_tag="ACHBKA_000908"
     CDS             complement(18484..19752)
                     /locus_tag="ACHBKA_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9444617.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(19983..20720)
                     /locus_tag="ACHBKA_000909"
     CDS             complement(19983..20720)
                     /locus_tag="ACHBKA_000909"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9444618.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(20787..21356)
                     /locus_tag="ACHBKA_000910"
     CDS             complement(20787..21356)
                     /locus_tag="ACHBKA_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9444619.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            21484..22356
                     /locus_tag="ACHBKA_000911"
     CDS             21484..22356
                     /locus_tag="ACHBKA_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9444620.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            22698..23570
                     /locus_tag="ACHBKA_000912"
     CDS             22698..23570
                     /locus_tag="ACHBKA_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9444621.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            23567..25525
                     /gene="nagE"
                     /locus_tag="ACHBKA_000913"
     CDS             23567..25525
                     /gene="nagE"
                     /locus_tag="ACHBKA_000913"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM9444622.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(25857..26708)
                     /locus_tag="ACHBKA_000914"
     CDS             complement(25857..26708)
                     /locus_tag="ACHBKA_000914"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9444623.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            26831..27298
                     /locus_tag="ACHBKA_000915"
     CDS             26831..27298
                     /locus_tag="ACHBKA_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM9444624.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(27561..28043)
                     /locus_tag="ACHBKA_000916"
     CDS             complement(27561..28043)
                     /locus_tag="ACHBKA_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9444625.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28143..29006)
                     /locus_tag="ACHBKA_000917"
     CDS             complement(28143..29006)
                     /locus_tag="ACHBKA_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9444626.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8389 a         6239 c         5354 g         9430 t
ORIGIN      
        1 gtccaatttt tgggacaatt tttttaactg ctcttcattc catggagaat tacgtcggaa
       61 atcaataaaa tgcatatctt tattttcttg ggctattgtt ttttccgttt tttttatcgt
      121 cttatataat tcataagcag aagttcgtaa tgctccttgg gaaaagatca tccactgttc
      181 tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat
      241 tctctcgata taactgtcag ccgtttcgtc ttctttcgta aaaacaacat ccaactggta
      301 tttcttataa ttttgttgga tgcccggaac tagttgggca tcgaagacaa cgatgacttc
      361 cattccttca tatttcgcat aatttgataa acgatgtaaa agagcttctc ttgcatccgc
      421 cattttgtcc tgctttttaa gctggacaag ttccggccac gaaccaatca tgttgtagcc
      481 gtctacaatc aacagctgtt ttttcatggt ctactccccc tgacgttggg aaaaagcctg
      541 atacataagc aaaccagcag caacactggc atttaagctc tggacgtgcc cggtcatcgg
      601 gatcgtcagc aattcatcca tttctttttt caatcctgga ctcattcctt tgccttcatt
      661 accaatgact aaagcaattg atcctttggc attccatttt tggaagtttg tgccggtcat
      721 atctgtgcca aatacccaaa atcctgtttc tttgagctgt tgcacagctt gtaccaaatt
      781 cgtcacgcgt gcaacaggga catgttcgac tgccccagtg gaagtctttg ttacgatagg
      841 tgtgattcca actgctcggt gttttggtat gatcacacca tcgacgccac ttgcgtctgc
      901 tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa
      961 ggggtcttct tttttctctt ttgttcgttc aatcagttgg tcaagggata aatattcata
     1021 aggtgtaatc gcaagaacga ctccttgatg cacgccgtga tcgctcaaag tttctaattt
     1081 ttgtttagga acccatttga ctggcacagc ttgttcacgt gcgatttctt tcagttcttc
     1141 tactttttct cctcgtgaat cttcttgaag aaagagttta ttccctcgcc cttgttgtag
     1201 cgcttctact gcggcatggt gcccaaagac gaaattatct tctaatgctt cttcattttg
     1261 atcagaaact gactgatttc gtccagtttg ctttttattt gaatatctat tttgtttttt
     1321 gtcttcaaat ccacgtttcg attgatgttt gtttgttctt tctttaccca tttttttcac
     1381 caactttctg aatacaccag cggatcaatt cttccatccg ctcggtttgt tttgtcaaat
     1441 gcagatagcc catgagcgct tcgaaccctg ttgcgacccg atacgtcgta acatcagcat
     1501 tttttgcgct cgtatggctt ttagcatttc ttcctctgcg ataataaagt tcttcttctt
     1561 ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa
     1621 cataattcgt tgccgcacga tgcagcttat tgggttttgt ttctccttga tcaatcaggt
     1681 ggtcacgaac atagatttcg taaatcgcat ctccaacata agccaaggct aaaccgttca
     1741 attgtgtgta atctctcatg cttctcttct ccatcgggtt ccttgcggtg tatcttctaa
     1801 taagatccct ttttctttca acaaatcacg aatttcgtcg cttcggacaa aatcacgatt
     1861 gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta ataactcttc
     1921 tgctgtaaat aaaataccga agatcgaaag ccattgcgag aataaagctt ctccttgtgc
     1981 caagacagcg gcagaaactt tcggctggtc tagataagtg ttcagccact tagcaagctc
     2041 atagaccact gtgatcccat ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg
     2101 ctgttctaaa ttttctaaca aagcgatttt atccgcatca tctgccaaat tctctgaggc
     2161 agtttcttgg cggaatttca aattgtcaaa cgtattcttc agtcgttgta aattggtggc
     2221 tgcttctttc atcgtcgttt cactgtagcg gatcggacga cggtattgtg tggtcgccat
     2281 gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt
     2341 ccctagagat ttactcattt tttcatcgtc ttcgccaatc gttacatagc cattgtgcat
     2401 ccagtaatta gcgaattttt tccctgtttt cgcttcactt tgtgcgattt cattttcatg
     2461 atgaggaaat tctaaatctt gcccgccacc atggatatcg atcgtttctc ctaaatgttt
     2521 tgttgccata acggaacatt caatatgcca accgggacgt cctttgcccc atggtgaatc
     2581 ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt
     2641 ttgctgctgt tcttcccctg tacgctgact tgctccgact tccagttcat cgatggattg
     2701 gtcacttaat ttcccataat tttcaaattt acgggtacgg taatacacat cacctgaaga
     2761 ttcataagca tagccttttt caaccaacac ttgaataaat gctaaaatat ctggcatatg
     2821 atccatgact cttggatgca gtgtagcagg ttcgacattc aacgctttcg tatcttcttc
     2881 aaatgctcgg atgaaacgat cagcaacttc tggtgctgta atacctaatt cgtttgctgt
     2941 acggatgatt ttatcgtcta catccgtgaa attcgatacg aaatcgactt tatagcctcg
     3001 atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg
     3061 gatataatta tacacagtcg gtccgcagac atacataccg acatgtccct tttgtatagg
     3121 gatgaattct tctttttttc tggtcaatgt attgtagatt tttatcattc tgatctcctc
     3181 actttttctc ctttgattcg aacgactttt gctggtactc ctaccgctgt agcgtcgtct
     3241 ggaacatctt tcaacacaac ggcacctgca ccgattttgg cgttttttcc gatcgttacc
     3301 ggtcctaata tctgcgcatt cgcagagagc atggctcctt gtttaacagt tgggtgtctt
     3361 ttacccgttt cttttcctgt gccgcctaat gttacgccat ggaataaaat cacatcgtcc
     3421 tcgatttctg cggtttcgcc aatgacgatt cccattccat ggtcgatgaa cacgccagtt
     3481 ccaatcgttg ctcctggatg gatctcaaca ccagttagga aacgccagaa ttgagcattg
     3541 atcttcgcca ataaaaagag ccggtgctga tataaaaaat gagagaaacg atgccaaaac
     3601 aaggcatgaa aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg
     3661 tttctctttg ctgcatcaat cgctcgggta aaccaactca tgactttcca ctcctttcgt
     3721 aagaatttta tgtaggaaat catcttctta taaagaaaag aagcgccttt taaataagtt
     3781 tccttatcaa aagacgcttt ccgcgtggtt ccacttttct tcataatcaa tgattatctc
     3841 tgacagctaa cgcctgttat acgttcttgg ctactttgtt tcctataaaa cagtttttcc
     3901 tgttttctgt gtttcgccaa gaccactccc agaggcattt cggcaaccat tcattgatac
     3961 cttgcaccac ccggtatctc tcttgtaatg atcagctgct tacttttttc tgttcatcgt
     4021 gtttagttca gtacttgatt taaatgtgct aatgattttt cacgaccaag caattcgatc
     4081 gtttcaggca attctggacc atgcatttgt cctgatacag ctacacggat tggcatgaaa
     4141 agattttttc cttttacgcc agtttctttt tgaacagctt tgatggcagc tttgacagtt
     4201 ggagcatcta ctacttccat ttgttctagc tgtgctttga atgcgtggag aacagtagga
     4261 actgtttcac cagcaagtac ttcttttgct gcttcattca atacaggatg ttcattgaaa
     4321 aatagcgtag acagatcaac gatttctgca gcatagctca tttgaggttg atacaggcta
     4381 acgatcttct tcacccattc gattgtcttt tcatctgggt tttctgctac acgtccatca
     4441 gcgattagat aaggcagaca catgtcagta aatgcatcta gatccattgc tttgatgtaa
     4501 tggttgttta cccattgcag ttttttcgca tcgaaagctg cgggtgattt acttaggcgt
     4561 tttggatcga acattttgat caattcttgg tgttcgaaga tttcgtcttc gcccacaggt
     4621 gaccagccca ataatgcaat aaagttgaac attgcatctg gtagatagcc taattcgcga
     4681 tattgttcga taaattgcaa gattgattcg tcacgtttgc ttagtttttt acctgtttca
     4741 ctgttgatga tcaatgtcat atgtccgaag gttggtggtg tccagccaaa tgcttcatag
     4801 atcatcagtt gttttggtgt attggcaata tggtcatctc cacgtaaaac gtgagtgatt
     4861 ttcatcaaat gatcatcgac cgcaacagca aaattataag ttggcatacc atcgcgtttt
     4921 tggatgacaa agtcgccacc gatattatcg gattcgaacg aaatagcgcc tttcaccata
     4981 tctgtaaatg cgtattctgt attgcgaggc acacggaaac gtacaacaga ttctagacct
     5041 tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga
     5101 ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa
     5161 tagcatttgt atgctcgatt gctagcaagc agctgatcaa tcagcggttg atagatttct
     5221 ttacgttcag attgacggta agggccgtat tctccaggat tttcaggaga ttcatcccaa
     5281 ttcattccca accaagcaag gttttccaac tggctttttt cgccatcttc gatattacgt
     5341 ttttggtcag tatcctcaat acgaataata aagtctccat cattgtggcg ggcaaacaaa
     5401 taattgaata atgcagttct tgcatttcca atatgcaggt gcccagttgg acttggtgca
     5461 tagcgtacgc gtactttcgt cattttcttc ctcttctttc aaactagact ttgactaaca
     5521 gcaaaaattc cgtcagttac ggcttctcaa atatcattta tttaaagcca ctcagcacaa
     5581 attttacctt ttctaaggta aatagtaaag ccaactggtt gaatttcctt accagttggc
     5641 tgaatttctt ttttattttg attgtttagt ctttttctct gtctcttttt tattgtcgat
     5701 tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc
     5761 acttgttaca acgacttcga tacgttcatt catgtaatgc tgtccatctt ctacgacaac
     5821 catcgtgccg tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg cttttactac
     5881 tagaacattc atcgtttcac cagggatgac gactggtttt acggcatttg ccaacgcatt
     5941 gatattaaga acaggtacat tttggaattc agagacttta ttcaagttat agtcattggt
     6001 tacaatgaca ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta cttcactgat
     6061 atcttcaaag tctccatcgt acatttcgac agatataccc tcttcttttt gcaaggcatt
     6121 taggatatct aatccgcgac gaccacgcac acgtttcaaa ctatcccctg agtccgcgat
     6181 gtattgtagt tcgtaaagaa caaaattagg gatcatcaag gttccttcta agaatccagt
     6241 ctttgtaata tcataaatcc gaccgtcaat gatcacgctt gtatccaaga ttttatattt
     6301 atggaaattt tctcctgatt ttcgttctaa cagttgactc tcaacttgac tagctgtttc
     6361 ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt
     6421 tcccattctc aatcccagat agcctaagat gatcatcagt aaaatcggca atacactatt
     6481 gacgaatgga atttccatat tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc
     6541 gatgatcgca ccgatcgcac cgaagagaag ataagtcaag ctgacctcac ttagtttttg
     6601 ttctacttgt ttgataatag ctgagatatg tttggccaac agcaaagata ataaaaagaa
     6661 aataagtgcg cctaacaggc tgttggtaaa tgtattattc agccatccgt ttgactgttg
     6721 accgattgcc tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat
     6781 catcagcaaa gtaatgacac gtttttgcat cgtttttcct ccgtttcgac aatcatttat
     6841 tagttagcgg aacacacgct tcaacgtttc agccaaagtt gctactccaa ctatctcgat
     6901 tccttttggc gcttcccagt tacctaagtt gtttttaggt aggtatactt ttgtgaagcc
     6961 gagtttttgg acttctttga ctcgttgttc aatatgactg acacgtctga tttctccagt
     7021 cagtccaatt tcaccgatga aacattccgt tggctgtgtt cctttttctt tatagcttga
     7081 agcaatactg acagcgatag ctaaatcaat cgctggttca tttaatttta ccccacctgc
     7141 agcttttaag taagcatctt gattctgaag aagcaaccca gctctttttt ccagcactgc
     7201 catgatcaga gatacccgat tgaaatcaag acctgttgtt gttcgtttgg catttccgaa
     7261 catagtaggt gtgactaatg cttggatctc taccaaaatt ggacgtgtcc cttccattgc
     7321 cacaacgata gcagagccag ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg
     7381 attagcgact tcttccaatc catgctcatg catttcaaaa atcccaatct cattcgttga
     7441 gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa
     7501 atataacacc gtatccacca tatgttctaa cattcttggg ccggcgatcg agccttcttt
     7561 ggtaacatga ccgacgataa aaatggctat cccatttgtt ttagcggttt tgagtagttc
     7621 tgcagtggtt tctcttactt ggctgacact tcctgccaca cttgtgatgt ctggctgcgt
     7681 catcgtttgg atggaatcaa tgataacata atcaggggag atatgttcga tggcacgact
     7741 gatttcgttc atatctgttt cagcgtatag ataaaattct gtgttgatac ttccgaggcg
     7801 ttccgcgcgt aattttatct gctctgcgct ttcttcgccg gatacatata agacttttcc
     7861 gcctatggct gctaattgct gagaaacttg taacagcaaa gtggatttcc cgatacctgg
     7921 atcgccacca atcaagacca tagaccctgg caccacacct ccgcctaaca cacggttcag
     7981 ttcttctaat tttgttttga tccgaggttc ttttttaggg acgacgtctg cgatttttgt
     8041 tggttttgct ttttctcctg tcaaacttgt ccgagttctg cggtccgttg tatcttgttc
     8101 gatttcttcg accatcgtat tccattttcc gcaatttggg cagcgtccca agaacttagg
     8161 cgacacatac ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg ccattgtacc
     8221 gcctcctttt atcctaatca tgagacatta aatcaagctt aagtatcttt aacgttttat
     8281 ttactggaag aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg atctgctttt
     8341 aaaaacggca aaaagatccc ttggccgata cgctcgcctt ttttgatcac tacatccttg
     8401 aaaccaaagt tggtgaattg gaacatgata tggccttcat ttgcctcatt attgtaataa
     8461 tcactgtcaa tcacacctac tccgtttgtc aacactaaaa accgcttcaa cggattgctt
     8521 gaacggttag caagctgcaa aaattcatct tctcccatgt aagctttgat tccagtaggc
     8581 acgagggttg gtttcaattg tttttcattt tctgatacat cttttttcaa tccatctgcg
     8641 actgtttcca accccttttt ccagcctgct ttccagatac ttggaatcac gacctcttct
     8701 gctgcttcaa aatcatagcc agcagcatga tgtgtagcgc gctgcggtag attgatattt
     8761 tgatctttat atttagaaat aacttcaaat cctctttttc tcaccggttt tcctccctaa
     8821 ataaactact cctattctaa catatccgag gctgtaaaag actttcttta caaattcttc
     8881 aagaaattct gctatcttaa agtaaaccat taattcgttc tttttccaaa cgactgcaag
     8941 ttgtacaaaa aaactatttt ttacatgact ttactcgtca aaacaagtag aatagaacga
     9001 aattaatgag tggatatttg attgaggtgt cacatgcgta aagaaattaa aaatgcaaaa
     9061 cgaattattg tcaaagtagg aacaagtaca ttgatttatc ccaatggaaa tatcaatctt
     9121 gcggcaatcg atcagttagc ttttgtcctt tctgatctta gaaatcgggg aaaggaagtg
     9181 atcttagtct catcaggagc tgtaggcgtt ggtatgcata aattacattt gtcagaacgt
     9241 ccgactttga ttcctgaaca gcaagctgcg gcggctgttg gtcaagccga actgatgaat
     9301 atctataatc aaagattcag tgtatataat caacagacgg cacagatcct cgtgacaaga
     9361 gacgtggtag attatcctga aagtcgtaaa aatgtgagga atacattgga acagcttttg
     9421 attatggaca cgatccctat cgtgaacgaa aacgatactg tagcagtgga tgaattagat
     9481 catctgacaa aatttggcga taatgatcag ctgtcagcca tcatggcaaa acttgtcgcg
     9541 gcagaccttt tgatcgtcct ttctgatatc gacgggtttt attcagataa tccgactaca
     9601 aatcccgagg ctcagatgta tcgctatatc gataaaatcg atgaaaagat tctggcacaa
     9661 gcaggtggtg ctggtagcac atttggaaca ggcggtatgc agagtaaatt aaaagccgct
     9721 tctaggatat ttgaagcaaa cagcgcaatg attttagcaa atggaaaaaa tccggcaatt
     9781 atttttgata ttcttgcagg aaaagaagta ggtactttat ttaaggagga cttgaaatga
     9841 ctgacttgat gcaattagga caacaggcaa aagatagcgc gcaacaacta gcacttatgc
     9901 ctacaaaccg caaaaatgag ctacttgttc aaatggcaca aaccatcaaa aagaatcaac
     9961 aagcgatcat tgaagcaaat cataaagact tagaaaaagc agtcgaaaac aatatcagtg
    10021 aaacgatgca agaccgcttg cagctgacac ctgagcgaat caatgccatg gctacagaga
    10081 tcgaaaaaat tgcttcgtta gacgatccta ttggaaaagt agatgagatg tggacgaaca
    10141 ccgacggatt gagaatcggc aaaaagcgcg tgccactagg cgtgatcggc atcatttacg
    10201 aatctcgtcc aaatgtcacg acagatgcag ctagtctagc attcaaatca gggaatgccg
    10261 ttatccttcg cggtggcaaa gaagcattct tctcgaatca attattagtc caattattgc
    10321 agcaagtact ggtcagtgct ggcgaatctc cttatgccat ccagtttgtc gatgatactt
    10381 ctcatgaaac tgcaggacag ttgatgcgat tgacagaata cttagatgtg ctgattcctc
    10441 gaggcggagc aaaattaatc caacgagtca aagaacaggc aacggtacct atcattgaaa
    10501 caggtacagg aaataatcac gtatatatcg ataaagaagc acagttatct atggcagtga
    10561 acatcatcgt caatgcaaaa gcttcccgtc catctgtctg taacgcggca gaaacattgc
    10621 tgatccattc tgagatcgca ccttttttct tgccagctat tgaaaaagaa ctggtagaac
    10681 acggggtctc tttacgtgcg gatgctcgtg cgttagaata tctagagacg gctgctttag
    10741 cagaagacac agattgggat actgaatatc tagattatat ccttgctgtc aaagtggtcg
    10801 attctttgga tgaagcaatt gcacatatca atcggcataa tactaaacat tcagaaacga
    10861 tcgtgacaga tagttatgcg gctagtcaac gtttcttaaa tgaagtggac gcggcggctg
    10921 tctatgtcaa tgcctccact cgttttacag acggatcggt atttggtttt ggtgcagaga
    10981 ttggtatctc tactcaaaag ttgcatgccc gtggtccaat ggggttaaat gaattaactt
    11041 ccacaaaata tatcatttat ggcgatggac aaatccgcga ataatgtttc gtgtgaaaca
    11101 ttattcccgc cttttgcctt ttttctgtaa aggttctatc caactagaaa ggtgtcaata
    11161 tgtttgaaat tttaatgatt ttttttattt accttatttc tctgaatatc gctgcttttc
    11221 tgggagtcgg tattttatcc cttttctttc aattcaaaaa acgaagcata gggagtcaac
    11281 gggaaaaatg gtcgcagtat tttgacaaaa tcgggcaaaa agggctggtt acacgcttgc
    11341 acattagtta catggttgca ttatgcttat tagcgacaat caattactat tcttttttcg
    11401 atcattcaat cgcttatacg attacgctac tgattgctgg gatttttcac ttatcttata
    11461 aatatcagct gaataaaaac cacttgaatc ggactttccg ctaaaaaata tccgtacgat
    11521 aagtagagtg ccttgaagat cggcaaaaaa ctgccggact ttttagaggc tgtgacaaag
    11581 attttgtcac agcctctttt tccatataaa cggcgttcgc ctaccagctg cctggtaaat
    11641 ctgtttcctc ttcgttaaga agcgaaaatc cgtcaaatgt ttcgccatca taataacgaa
    11701 gcagctcatc catggacaag ttcatcgttt tttctttttc ttcttccgct gattcagcca
    11761 gtagctgttc ccgatacacc tgatacatac cagctatttt ttgctgcatc gcttttctag
    11821 tttcaccttc cgcattgatt attggcaatt ctttgatata gccacgataa atcaaccgat
    11881 catctgaaaa ttcaatgggt aattcaacga gtgtcaaccc gacttcctga aaatattctt
    11941 ccagcatcta ttatcccccc attctttttc ttaaatgaat tttagcttac ctactgaaaa
    12001 ttttccagtt tcattatttt gagaaaaaaa gggaatgtga caaaaatcgt gtcacatccc
    12061 ctttttccga ataaatggtg ttcttctgcc aactcctcaa tcataagtca caatattcaa
    12121 caaacatgag aaactgtttt ttgaaattgt tccttgtgat tcagagttaa acgacagtct
    12181 cacaacctct tggtaaacgg cgttcaatca gctgaccctc ggtattagct caaaaaacgg
    12241 taaaatatga gaagctattt ttccgttttg tttctctaat acttaggata aaacgctgat
    12301 tttacaacct ctgttaaacg gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg
    12361 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggaca aaacgctgat
    12421 tttacaacct cctgataaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    12481 aacgaaaaat atgagaagct atttttgttt ggctgttctt atgacttgat gcaggacagc
    12541 tttttcacaa cctctgattt cttattcttc ttcatttgta gcaaaataat tacggtgata
    12601 aatgatacaa catacactga taagaacaat ggcacctgca aaataaaacg catagctttc
    12661 accaatatgt tcaccaatca cacctgcaat aaatggtgct aaagctccac cgaaaaagcg
    12721 gacaaaacta tagctggacg aagcaacgtt tcgttgcaga cctggtattt ccatagcaat
    12781 cgtcgtcaac agtgtattga taattccttg gaaaaatcct gctaacacaa tgctgatcgg
    12841 tacaaggact ggcatagaag ttccagctcc taataacagc aaacaaataa tgaataagac
    12901 aaatgctgct aaaattgtcc gataagtcgt aaacttccgc tcaagagccg gtgcaacgaa
    12961 aatcgaagca atagccagaa gcacacccca gccaaaaaat acaaaaccga cttctaattc
    13021 agaatagcca atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc cgaaattata
    13081 taacaatgcg attagtccaa ctgaacgaag tttatgattg cttaacgctt tgacaccttc
    13141 ccaaaggcct acttttctct ttggtttctc agtttcaggt aaaagtaccg ctagggctat
    13201 aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga taacgccatg aaccactgcc
    13261 caagacccca ccgactagag ggccaacagc cattccgcag ccaagtgctg cttcatacat
    13321 catgattgct ttttctgtgt taccaactaa aatactgacg atcgttgcta aagcagtaga
    13381 gacaaataaa gcattcccga caccccagcc ggcacgtaac ccaatcagca ttccgatatt
    13441 cccagataat ccgccaagac cagcgaatac aacgatgata aacaatcctg tcatcaatgt
    13501 ttttttcaaa ccgatccgac tagataagaa acctgtaaat agcatcacca caccagtgac
    13561 taacatgtaa ctagtaaata acagggtcgt ttcagctggc gtagcgttta actgtgcagc
    13621 aatcgttttt aagattgggt ctactagacc cactcccata aaagcgatca tacaggtagc
    13681 tgctacaact agcgctgaac ttttcccctt tgtttcttca tttgtttgtt cttgtatatc
    13741 tatttgattc tccaatattt ccatcccttt ctctctatta gcatcataca tgatttattt
    13801 tacatgtcaa tatgcacatg taaaacgtgg aaactttact gtgatataat aaagaaaata
    13861 agacgagagg taggattttt atgaatactt tagcctatat attattatgc gtgcttttat
    13921 ccaaaccacg ttctgggtat gaattgaagc agctgatcac aattttttgg gaagcccatc
    13981 acagtcagat ctatacaacg cttgccaagt tagaccagca gggttatgtg accgttttgg
    14041 acaaagacga gcactctcaa aaaaagatct accacttgac tgatgaagga cgtgctcttg
    14101 tagaggaatg gatcaaagag gaaactcccg ctcctacgca aaaagatgaa tttctagcaa
    14161 aaatctatgc tttttcgacg ctcgataaaa caacagctac tggactgcta ttcactagag
    14221 aacaacagct gaacaaaatc atgcaaaaaa atcaaaaaaa attgaatgac ctctcgctag
    14281 acaagaaaca agaatttgga cgttatgttg tgatccagcg cagaattatg ctttgtcagc
    14341 aggaactcca atggtgtcag caagtaagag aacaaatgca gcagtttttt gagtaaggga
    14401 taaacagaac ctttctagaa atatctattg aagagcaaat tatcttcaat aaaagagcaa
    14461 tacagttgga aaagaaaaag cgatccgaac gacaggccaa aacctatcgt tcggatcact
    14521 atttttctag aactgcattc aagagactat gacaaaaagc atgccaaggc cttttttcaa
    14581 ataacagtgt tccatcattc attactttga attgctttta gcacacttct tgaaagcggg
    14641 ccaacgatga tcaactgcaa cggtaaagcg gcaatcacat tcattttcca agcattcata
    14701 taagcagaga ataattgatc aggtattcct tgttccatga tcacaccgaa taaagacata
    14761 catgtcacca ttcctaaaac catcaagcat gaaatagtta agatcatttg gaccttgctt
    14821 tctggattta ctggtagttt aaaggcaatt tttttcgcca gcacaccgat gatgaagaca
    14881 tccaaaatga aagccaccac gaatccaggc actagaccaa tcaacaaact tgataaatga
    14941 agatcttgat gcagccataa attatacgcg ctcatcccca agaccatcaa aaaacacatc
    15001 attgttgtaa aaattattcc ttctttttta tttgtaggca atctgtacca ctcctttaaa
    15061 tttgtcacga aaatcatcgt agcacattta aaaatttctc gtaagtactt tttttatttt
    15121 ctgccctttt atttcataaa cttcatcagc cgtacgctcg acaaagtaag aatcatgaga
    15181 agtaaacaaa atcgttcccg gatatatttg cattagcttc tctaaagctt cgatcgttgc
    15241 catatcaata aaattagtcg gttcatctaa cagcaacaca ttacttggct tagtaaaaag
    15301 caaagcaatc gtcagacggg tcgcttctcc cccacttaat gtacataacg gtttcgtcaa
    15361 agtttcctga gcaaacccta agtgattcag caaactacgg acaagcgatt ctgaaaactc
    15421 tgtttgtctc atgacaaaac gaatgatgga ttcttcagat gtcatttgat aacccttttg
    15481 ctgatatact tgaaagctta ctttcggaga gagaaggatt ccttctcctt gtttgcggat
    15541 atgttctaat aaggttgtct tacctgaacc attttcaccg acaatcgcga tttttttacc
    15601 taaagaaaac tgaaaatcac catttaccag taatgttctc ccacttcttt ccaattggac
    15661 attttgtccc atgatgggat agcggctgtg gagttcaaga gattttggta ctggaaaacg
    15721 gatttgtttc atctgctgcg gcttggttgt ttctcctatt ttttggagtc ttctctctaa
    15781 tgttttcgca ttcttttgga tggctttttg tacactgtct ttttgtttag aggaagacaa
    15841 ccgatccagt ctgatttgtt gctttttctt tttactggac actttttcca acttttccgc
    15901 ttgttttttc ttttcttgga tcgatttttc caatcgtttt ttctctttca tgatcgactc
    15961 tgcttctcgt agctgagtct ttttctccaa ttccttttga gtgagatagg cactataatt
    16021 ccccgaaaac tctcggattt ctccgtcttt tacttcccag attttcgatg ctagctcatc
    16081 tagaaaatag cgatcatgac taacaaaaac cagcgtacca taataataac gaagctcttc
    16141 aatcagtcga tcaatacttt ttctatctag atgggttgtt ggctcgtcta gaagcatccc
    16201 ttgttcataa gaagaaagaa gacgtatcaa ctcgatcttt cgttcttccc ctccgctcca
    16261 tttccgctct gaagtttctt ccagtcttaa ctggaaatga ctggctaatt ccaaatttag
    16321 gtcctttgct tcagcgagat aggttaattg aggcaggtag ttgaaggtga tctctgtttg
    16381 gattttccca tgatcgggaa aaagttcacc ggctattagt ttgagcaaag tcgatttacc
    16441 gacaccattt tctcctatga tccctatacg atcttgctga tagacacgaa gagaatcaat
    16501 agatagttct agctggttgc caaagctgac ttgtagattt gttatattta ctgctaaatt
    16561 ttccatatgt tttacactcc tatttcatgt aaaacaacaa ttatcgatac taaaaaagag
    16621 cagaaaaatc tgtttgcaaa aaaactgcat cacatctttt cctactctga acgaaataac
    16681 tatgactaaa tcaccaaatc tgtcatagct ttcacctatt ctactgtcgt tcgaaaaaat
    16741 ggacatactc catccatgaa attgttgttt atacatgtct tttgaattta tttagttgtt
    16801 attcaaaagt tcgaaacgta atttcatcga tgcagtcatt tttccgacct ccttttttat
    16861 taggttacca cacacgattc aaaaaaagca atagctactg aatagtaact gaaaaccttt
    16921 tattcgaaca tagtgatctt ttctggtgct aaacgaacag actcgtaacc ttcttctacc
    16981 gcttttccta ttgataaaat aataactgga acataacggt ctttatccaa accaaatgcc
    17041 tctgccaact gatctgcttc gaatccacca attggatttg tttcatagcc atgatcacgc
    17101 gcaactaaca tgaattgcat agccgctaag ctagaatcga ctttgactac atcgttcatt
    17161 tcttctcttg aaaaactctt gtaataagga atgatcgcag ctaattgttg atctctgact
    17221 tcttttggca tcttccctga ttcgtaagct tggttgtaga tttcttcgcc atattcgtaa
    17281 cattgcatat cgccaaaaat catgaccatt gcagaagaag tatcattttg tctcgtattg
    17341 aaacggatca aaggacgcaa gatttctttt gcttcctcgc tttcagcgac cacaaagcgc
    17401 catggtttca tattgacaga agaaggtgca gtcgtcgctt ctttgatcat ttccagcatt
    17461 tcttcgtgtg ggattttgta tgtttcatca tatacgcgaa cggatcttct accagtcata
    17521 attgttgaaa aatcattttt ttgttccatt attattaacc tctttcgttt ctgttgttat
    17581 attgcaaagt tgggtattca atttatctaa aagtgttaga aattgtttca tttctacatc
    17641 gctgaaagcc ggggatagca cggaacctgt atctttatgg tccatttcac aatgcttgat
    17701 tttcttctgg gctttttctg ttagcgctac atacacttcc cggttgttct gaggatttct
    17761 ctgacgcgaa acatagccat ttgcttccaa taccttcaga tgacgagtaa ctgctccttg
    17821 atcaatctgc aacttttctt gcaaactgct ttgtagcaat ggattttctt ctttcagtct
    17881 catcatgatc tcgtatctag taagacttat accgatattt tcttcaaaga tctggacgat
    17941 tttttgatcg gctaatttga tttgatataa aaggtgacta atctcttcta aagacataat
    18001 ttcctccttt ctaataattg actcgtcaac gattgacttg tcaataatag aataaaaaaa
    18061 gaaaaaagtc aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga aaagtataga
    18121 gaaaagaaat tttttgtaga atgaacggaa aaaatgataa agaaagcaca acgctaaaac
    18181 caagattatt gattcttatc aaactataga agatagctct tctgcagtag ataattttac
    18241 accgccaaaa atttccgaaa tcgccttttg caagaatccc gtcgaatatt agaaattgga
    18301 tcagtatgct taaacagtaa taaaaaagtt atgacaagat gttgtcacaa ccttttttga
    18361 gtaaacggtg ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca aaaaacatga
    18421 aaatctgttt ttgtttggct gttcttacta cttgatgcag ggcagctttt tcactacctc
    18481 tttttaataa ccataaaatc ctattccttc atatctccag cccatcggtg ttagccaatc
    18541 tctttcctta gcactcaagg tatagtgatg ggtaccagtt ttttcatttg gattaaatag
    18601 tcgataaatt ggcttggaac caccagaata aaaacttaga ccttcataac gccaaccgat
    18661 ttttgttaaa gagttgatct cagattgtgc cattgtgtaa tgatggtctc cactgtaagg
    18721 attgtataaa cggtagacag gttgtccgct gtttggtgct tgccaagcaa tcccttcata
    18781 ttcccagcct acagttggca gataattttt ttctccggca ttcaatgtat ataaatgttc
    18841 tcctgtatga cggttataca gacgatacat cggagaagtt gaagttggtg ctgtatcata
    18901 ctgtgtcaga ttataggttt cgattacatt gttcaatgta gaaccataat taggcgcagt
    18961 ggcataacga cctgttagcc aagccgtagc atctttgtaa gagttcgtat tgcttttcca
    19021 tgcaccggaa tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata ccgttacgtt
    19081 atcttgcaat gattctttgt acgaaggata ttttctaaaa gcggcattca ccgtaatcca
    19141 ttgcccattc acatattccg aagtaggcat cgtaacagat tggccattat agcttccttt
    19201 aatcccaaat agattgtaat taggggcaga agccaaagta ctgttccccc agccactttc
    19261 aacgatggct tgagcaatca tgactgatgc atataagtca ttggcacttg ccaaactttg
    19321 tgcagttgag gcaatactat caatgaacgc ttgttgagaa ttagcagatg agcgagcata
    19381 aaaagcatag ccagtatagt tttcgaattc agacaaatct gcttgatggg tatgtgtcaa
    19441 tgtttgccca tctgtcaatt ggttcgtgat ttcttctgca ttctctggat tgttctgagc
    19501 ttctgctgat gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg ttggttcttc
    19561 ggttgaaatt gtattttctg agacagtagc tgactgcaaa gtactttctg tatcgttttc
    19621 aattcctgtt tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg attcttcagc
    19681 tagcactgtt gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa ataataaatt
    19741 ctttgctttc atcttttttc tcctttgaat tatgaaactt ttcctaaata acagtgtaac
    19801 taaaaaagta aaagcaagtc aatgtccata tttaaataac atttgtttct ttttaataaa
    19861 tttgcttttt ctgcataaaa agacgtaaat gaagaatatt cttctccatt cacgccttaa
    19921 attgatggtc gtttgaataa ctatttaaca agtaattctc tctggatatt atccgttatg
    19981 gattatactt cctgtgtcgt cggacggata atcatgttgc tgacagacat ccggtctggc
    20041 gtttctatcg caaatgctac agcagaagct atatcttcag atgttaatcc ccattctttt
    20101 tgcgcttcat gaagttcaag agcagccttt ttgtcgctga tggtttgata taattcagtt
    20161 tgaacagctc ctggagagat gatcgtagat ttgatgttat tttgccgttg ctcttgacgt
    20221 aatccttcca tgattgcacg gacagcaaat tttgttccgc aataaacggc ggaatcagga
    20281 taaacaacat gtcccgccac agaatcagtt gaaatgatat gcccagattt ttgtttttcc
    20341 atgattggaa gtacggcgga aattccattt aacacaccca tgatgttgat atcaagcata
    20401 ttttgccatt catcacggtg accttcaact aatggagcgg ttggcatgat tcctgcatta
    20461 ttgtacaata catcgattcg accgaacttt tccatcgcta aatcgataac tgcttggact
    20521 tgtgcaaaat ctcttacatc tgctgtttga atatatagct cagcttctgg taaagattct
    20581 tttattgctt ttaggcgatc ttctcggcgt gcagctataa ctagtttagc ccctttttct
    20641 gctaatagct tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat gacaatcact
    20701 ttttctgata aatcattcat tctattctcc tcctgttttg aaaaatctct tgcttatact
    20761 cactatctct tatttcatct tactgttcaa ataatccgtt cgacaggttt gacgattttg
    20821 gcagggattc ctgcaacgat ggtattgtct gggacatctt tcgtaacagt tgcattcgct
    20881 gcaacaatgg cattctcgcc aatcgttacg cctggcagaa tagttgctcc tgctccgatc
    20941 caagcatttt tcttgatgtg gattggcttt actgacaagc ctcttcgttc ttttggatcg
    21001 gttagatggt tcaccgtgat gaggttgaca cgaggaccaa tcaatactga atcttctaaa
    21061 cagatgccac ctaagtcaac gaacatcact tgacgattga taaagacatt tttccctata
    21121 ctaatatgtt tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt atcatctact
    21181 ttgtcactaa tgatattttc tagccgtttg cggatatctg ctggttcatg gtacccagta
    21241 ttcagcgctg ctaaaggctc ttcgttttcc gcttggatct gatggatcac ttcaaagagc
    21301 ggatccgtta ctcgaatctc tttattgacg atttgttttt cgatgaggct tgtcattttg
    21361 ctccctcctt ttatccttac tttataacgg acggcttatg atgtctaata cttataaaac
    21421 atacttaact attcctttta actatactta gtggtatgct tttttaaaaa caggagggaa
    21481 aaaatggata ttcgtatact gacgtatttt ttagcagtag ctagagaaaa aaatatcagc
    21541 cgagcagcaa aatctctgca tatcacgcaa cctactctct caaaacaact gaaagattta
    21601 gaagaagaac ttggtgtgac acttttcact cgaggctccc gagagataca gttgacggat
    21661 tctggtcgat atctttattc aaaaggaaaa gaaatcttgt cgttggtaga aaaaacggcg
    21721 aacaatttaa caaatgacgc aatcatctct ggagaaatct acattggcgg aggcgaaact
    21781 aaagcaatct ctttcatttc tgatagcctt cattcgttga tcaaggcgca tcctgatatc
    21841 cagtttcatt tatatagcgg gaatgcagat gatatcatag acaaactgga tagcggatta
    21901 cttgattttg gactcgtaat cgaaccgaca aacaaacaaa aataccagtc atttcgactg
    21961 ccagcccaag atcgttgggg acttttgata catcggaatc atcctttagc aaagcaaaca
    22021 gtcatccatc cgcaggaact gaaaactgtt cctttgatca tttcccggca aacgtcagtg
    22081 gacagtcaac tagctgaatg gctaggtttc agcatagatc agttatcgat cgtcggaaca
    22141 tataacttat tgtacaatgc ttctttgatg gtcgataacg ggacttttgg tgcattagct
    22201 ctagatggga tcatcaatac ttatgggacg gaaaaagtat tcatcccact cgatccgcca
    22261 ttacaagctt cactgaatct catttggaaa aaagaccagc ctctttctcc tgctgctcgt
    22321 aaatttttag atgtcctaac ggagaaatac agttaagtag agtttttttc cttcgctttc
    22381 agctttttaa caacgaataa gttaagcaaa agatccaatg attaaataac ataagtttct
    22441 taatttgttc cttcctttta tttctgataa ttccagtaaa ttactataat tataaaaaaa
    22501 gaattgacaa aacataactg tctatataaa attgattacg ttaacaataa ttcgtttcat
    22561 gtgactagat catactaggc atgaaaaatc tctgaggaac atggtttccc taggaggttt
    22621 ttcgtgtcta tttttgtttg gccgcaatca actggtctag caaacgtgaa aaccaaatct
    22681 tcaaaaaggg tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca gtattggtag
    22741 atgacggtgg acaggaaaaa gtagctattg gaaaagggat tggatttgac agaaaaagaa
    22801 atgatttgat ttttgatcgt gatgtcgaac ggatatttgt catggaacca gaaggtcaaa
    22861 tcaagttgca aaatttactt aatcagattg atgaacgctt tttatttgca gcagaaacga
    22921 tcattgatca tgcggaaact gttttgatgg aaaagttgaa tgaacatgta ttgatcgctt
    22981 tgaccgacca cattgctttt tctgcggaga acattaataa cggtatcgtc atccgcaaca
    23041 aacttttaag agaaatcgaa gtgctttaca gtgatgaatt cagtattgct caatgggcag
    23101 tagagtatct gacaaaagag cttgggattc catatactta tgacgaagca ggatatatcg
    23161 caatccatat tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt atccgtgaag
    23221 tgacgattgt atctgacatc atccatttag tggaatcaga attggaaaca gatatccatt
    23281 cagaaccatt ttcactgaat tattcaagac tggtgaatca tttacgactg cttctacaac
    23341 gaacccatgc gcaacaatat gctgttttag acacggaaat cgtagaaatg gtcaaacgaa
    23401 aatacccaga aagctataag atttctaaaa aaatccgtgt gttgctgacc aaagaatacc
    23461 agctcgcgat cacgaaagaa gaactgggtt acttggccat ccatatcgag cgtttaagaa
    23521 gcgcaatcgt acaaacaaat gttaacaatc atgaggagga aaaaaattga aagcatatct
    23581 acaacgaatg ggacgttccc ttcaacttcc agttgcggtc ttacctgctg ctgctttact
    23641 agtagggatc ggaaactggt gggcatctta tagtaacgac attgtcgctc attttttaca
    23701 agctggcggt aacgcagttt taggtcaatt acctttgctg tttgccgttg ggttagcact
    23761 agggatgtca gtggacaaat caggtgctgc tgccttggca ggtttggtcg catttgaagt
    23821 tccagtaaat gttttaaaaa ctgattcagt agctacttta ttgaatatca aaccagaatc
    23881 agttgatcca tcttttgctc agatcagtaa cgtatttatt ggtattattg ccggattgat
    23941 tgcggcagct ctttataatc gtttccacga agcaaaatta ccgatggctt tatctttctt
    24001 tagcggcaag cgtctagtgc caatcttggc tgcctttgtc atgttgctga tcagtgcagt
    24061 gctactgctc gtatggcctc cggtttactc tgctttagtt tcttttgcta aattcatttt
    24121 gagtttaggc tggggtggtg caggtcttta tggcttcttc aaccgtttac tgatccctac
    24181 aggattgcat caagcaatga actccgtgtt ctggtttgac gtagcaggaa tcaatgatat
    24241 tggtaaattt ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc aagctggatt
    24301 tttcccaatc atgatgttcg ggcttccagc aggagcatat gctatttaca gaaatgcacg
    24361 tccagaaaac aaagcaaaaa cagcttcctt gatgttagca gcagcttttg cttcattctt
    24421 tactggtgta acagaaccac ttgaattctc atttatgttc gttgcttggc cattatatgt
    24481 cttgcatgca attttcactg ggatttcttt agcagtgagt gcattcttcc attggacagc
    24541 aggatttgcc tttagtgccg gatttgtcga ttacttccta tctttgaaaa atccgattgc
    24601 gaatcagcca ttgatgttga tcgtccaagg attagtaaca gcagtgattt actactttgg
    24661 ctttaacttt gctatcaaaa aattccatct gatgactcct ggccgcgagg aagcagatct
    24721 ttcagatgac gatactgcaa cgactaattc agacaataaa tatgcggcac aagcaagcaa
    24781 aatctacgct gcattaggcg gagccgacaa tgtaacttcg atcgataact gtactacacg
    24841 ccttcgtttg caagtcaaag atacaggcct gatcgatcaa aataaaatca aagccactgg
    24901 cgtacctggt atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag gtacagaagt
    24961 acaattcgtt gcagatgaga tggcaaaact acatggtggt gctgcaacac gacctgcaca
    25021 aacaaacacc gtggttaaaa cagaaacatt tgccggtgga acgatcgaac aagatattta
    25081 cgctatcgca aatgggaaac tgattccaat cactgaagtt tcagatgatg tattcgcgga
    25141 aaaaatgatg ggtgacggct atgctgtatt accagaaaat ggagaaatct tctctccaat
    25201 cgctggtacg atcacaaaca ttttcccaac gaaacatgct gtcggaatcc aaacagatgc
    25261 tggtatcgaa gtcttgcttc atatgggcat caacactgtt gacttaaaag gcgaaccatt
    25321 cactttatat gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg ctttagttga
    25381 tcttgccgct attcaatctg caggcaaaaa cacggatatg gtcgttgtat tcacaaatgg
    25441 cgacaaagtc caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa atgataaaat
    25501 cggctctgtt tccaacaaag cctgagccat cgtcttcccc agactttttt atcgataaac
    25561 aaacaggagc tgagacaaaa atcttgtctc agctcctttt tccgaataaa aacggcgttc
    25621 tcctgccaac tccgcggtaa taagtccagc caaacgtaac atatgaagag ctatttttta
    25681 tttgactctt cttattactt gatgcagaac agctttttta caacctctga taaacggtgt
    25741 tcaaaaacca gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa agccattttc
    25801 acaaaatttt tcttcatact caaaactaat cggctttttc acaacctctt tatacattat
    25861 tcctgatcca atatttcttt taatgctgct tcaagagaag ttaatggttt tcccaatact
    25921 ttttctaaat catcagagga aatagctaac tgatcctttt taatatcatg ttggatcgct
    25981 aaaaacatgt cagctgcttc ttgagggaat ccggcttctt ttagattttc gacaaacgct
    26041 gcatcacttg cttcaataat ttctaacttt ttacctgtcg cacgctctaa tgccttggct
    26101 aattcttcat agtcagttag attgccggat aattctaaga tttctggaga gtcactttct
    26161 actagtactt ttgctgcagc ttctgcatat tcacgtttca atgcccaacc tgtttttcca
    26221 tttcctgcag cataaacgaa ttgaccagtt tttaaagcct gcccaatgat aggtaattca
    26281 ttctctacat accaattatt ccgtaaaaat gtatgggaaa tacctgaatc cttaatcatt
    26341 ttttcagtga attggtgatc tggcgctaat acactgatcg agtgatcggc atctgcaaaa
    26401 cttgtataag cgatataaga taccccagct gcttgtgctg ccttcactac attcttatgt
    26461 tcggcttgac gatttcctgg aacgccagag acaaaaagca atcgatctat tcctttcaac
    26521 gcttctttca ttgagtctaa atcatcataa tcaccaatac gtatattaaa tccagcttct
    26581 ttcagtgctt ctcctttttc ttcactacgt actagtacat agatttcatt ttgtggaacc
    26641 atctttttta agaattccag cgcataacca ccaaaacctc cagttgctcc tgttactaaa
    26701 taatgcatat ttgccactcc ctattctttt tatttgttaa gatttacagc tgtacattta
    26761 taaattacac ctttatcata aagagtgtgg taagataagt caagcaaaaa aaatgaaagg
    26821 atgacaactg atgaagtatt ctctgcaatt tagtgatgca atccatgtac ttgcctatat
    26881 tgaaatgttt caagataccc gatcactttc tagtgagatg attgccagta gtatcgaaac
    26941 aaatgcagca aatgtacgta aaatcatgag tcagctcaaa aaatccggtt tgatctttac
    27001 aaaaaccgga aaaccgcagc cttcccttgc caaaagtcca gatcagattt ctttactaga
    27061 tgtctatcgc agcatcgaag gtaatacaca gcttatccaa gtcgatccaa aaacaaatcc
    27121 aaattgcgtc gttggtgcga atatccaaga agctttagaa gaaagctacg ctcggctgca
    27181 gaaaaaggca gaagaagaaa tggctaagat cacacttgat agtctgatac ataaaatagc
    27241 cgttttggaa aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat ttttataatt
    27301 ctctctactg tgcctgcatt tttctgatcg gaatcgtcta cataaattct tttattctcc
    27361 gggggacaaa agccttgtca tgcccctttt tcctctctaa tgctcaggtc aaagcgctga
    27421 agtcatgacg tcttgataaa cggtgttcaa gtagctgacc ctcggcatta gttctgaaaa
    27481 atgcaaaata tggaaagcta tttcccattt ttcctctcta atgctcaggt caaagcgcta
    27541 ctttcacgac ctctatatta tcatggttta ttgactaccg gtatccagat ttctcccata
    27601 tatttcccgt tggtaactcc catttcgact acagcatttg gacccccaac ataggcgtaa
    27661 tcttctactt ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt tcttaattct
    27721 tcaggcgatt ctgctaaagc gattacaaca agatacgttc cttttgggaa tgggatgatt
    27781 cgggtagtgt ttggaacatc ctgactcgtt tgaacaccga catagtacat cattttcccg
    27841 ttatagggtt cgttgactgt aaagaaataa tgattttcag cagcttgttt caatgcatct
    27901 attgttccat taccgataac tttttgctca aatgcttgtt tctctgcagc caaaccgaca
    27961 aaatccgtat tgctactctt caattccaca ccaatacccg tcaccgtaaa atctggtttt
    28021 tcttcgatcc tatactttgc catttctccc accctttcta ttatattaag ttcatcttac
    28081 ataaaattaa agggaaatga cacgattacg ctcttttttc tagggatatt tttatttttt
    28141 aactattttt ttgctttcac tgcaatccaa atctcactgt aaacattact gaaatcctct
    28201 ccccattttg taaaagagat ctcgggagct tcgactaact cataatctga agaaggaagc
    28261 cattctgcat agattttccc ccaagtatct tgtaaagttt ggggaaatgg ccctttatta
    28321 ggaaaaatgg cccaagtgtg ttcttctata gcaagcattt ctaaatcagc gtacggattt
    28381 tcttttgtcg tagcaaagcc aatcatgtgc atcaaactgc ccttttcttc caagcgggct
    28441 ccatcaaatc cataagaaac gttgattact tgatttggat ataaatctcc taacttatgc
    28501 atctcttccc tttgagtctc cgtgatcgtc tgcgctaact tttgtatttc ttgattgact
    28561 ccttcaaatt tgacaggaac ttttttcgcc acacctgcca cataaaactg ttctttcttt
    28621 tcgatacgaa attccatcga ttttcctcct ctgacatcaa taaaaaatgt caagcgtgga
    28681 aaagattttt gtatgccgta tctgctgatt tctgaaggca gatatcctga ccaatcacga
    28741 aaagctcttg aaaaaccttc gactgtttca tatccatatc taaacgccgt ttctgtcact
    28801 cttgatccat ctaggagatc gcgattagcc aaagccaatc tacggttttt gatatagtcc
    28861 attagcgaca tccctgcaat aaacgtaaag atgcgtttta attgatattc agagattcct
    28921 gtattttcgg ctattatttt ataggaaagt ccttctgtca aatgttcttc gatgtaatcg
    28981 atcaattggt tcagatcttt aagcatattt atcttcctcc ttgcttgcct ttttatcata
    29041 ttatcaaaga aagtcaatta cttgatgaat caggaaccaa tttagtcaga tgtagaaaat
    29101 gttgtatgga tctaaagaaa attctcaagc agcgtttcgt aacggataat tggtattatc
    29161 tgcctatcta tgttttacct ttagaaaaat aaattttggg gaagacacat tctaagtaaa
    29221 caaagcggta aaaatatctt ttattccctt agttaatccg cttactacct actgtacaaa
    29281 gcaaattgtt gttcaaactg ctgatcagaa cgctgcttgg atttttcttt gcgatacatc
    29341 atacctcttt tatcaattgg aacaattatt tatttctaac aaaatcactt catttttgtc
    29401 agataaataa aa
//
LOCUS       DAYYOA010000022        27861 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000022 DAYYOA010000000
VERSION     DAYYOA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.022"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHBKA_000918"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHBKA_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM9444627.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHBKA_000919"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHBKA_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM9444628.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHBKA_000920"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHBKA_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM9444629.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACHBKA_000921"
     CDS             1642..2274
                     /locus_tag="ACHBKA_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9444630.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACHBKA_000922"
     CDS             complement(2276..3181)
                     /locus_tag="ACHBKA_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9444631.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACHBKA_000923"
     CDS             complement(3266..3925)
                     /locus_tag="ACHBKA_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9444632.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACHBKA_000924"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACHBKA_000924"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM9444633.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHBKA_000925"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHBKA_000925"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM9444634.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACHBKA_000926"
     CDS             6168..7253
                     /locus_tag="ACHBKA_000926"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM9444635.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACHBKA_000927"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACHBKA_000927"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM9444636.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACHBKA_000928"
     CDS             complement(8563..9366)
                     /locus_tag="ACHBKA_000928"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM9444637.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHBKA_000929"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHBKA_000929"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM9444638.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACHBKA_000930"
     CDS             complement(10244..11251)
                     /locus_tag="ACHBKA_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM9444639.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACHBKA_000931"
     CDS             complement(11258..11902)
                     /locus_tag="ACHBKA_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM9444640.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACHBKA_000932"
     CDS             complement(12002..12760)
                     /locus_tag="ACHBKA_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM9444641.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHBKA_000933"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHBKA_000933"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM9444642.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHBKA_000934"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHBKA_000934"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM9444643.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHBKA_000935"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHBKA_000935"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM9444644.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHBKA_000936"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHBKA_000936"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM9444645.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHBKA_000937"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHBKA_000937"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM9444646.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ACHBKA_000938"
     CDS             18158..19027
                     /locus_tag="ACHBKA_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9444647.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ACHBKA_000939"
     CDS             complement(19153..20052)
                     /locus_tag="ACHBKA_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444648.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ACHBKA_000940"
     CDS             complement(20217..21041)
                     /locus_tag="ACHBKA_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9444649.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ACHBKA_000941"
     CDS             complement(21052..22347)
                     /locus_tag="ACHBKA_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM9444650.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ACHBKA_000942"
     CDS             complement(22363..22842)
                     /locus_tag="ACHBKA_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM9444651.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ACHBKA_000943"
     CDS             complement(22848..23876)
                     /locus_tag="ACHBKA_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM9444652.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ACHBKA_000944"
     CDS             complement(23893..24999)
                     /locus_tag="ACHBKA_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9444653.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHBKA_000945"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHBKA_000945"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM9444654.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7854 a         6122 c         5035 g         8850 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa a
//
LOCUS       DAYYOA010000023        27689 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000023 DAYYOA010000000
VERSION     DAYYOA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.023"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(234..2126)
                     /locus_tag="ACHBKA_000946"
     CDS             complement(234..2126)
                     /locus_tag="ACHBKA_000946"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444655.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2123..3850)
                     /locus_tag="ACHBKA_000947"
     CDS             complement(2123..3850)
                     /locus_tag="ACHBKA_000947"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444656.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3866..4516)
                     /locus_tag="ACHBKA_000948"
     CDS             complement(3866..4516)
                     /locus_tag="ACHBKA_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM9444657.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4720..5016
                     /locus_tag="ACHBKA_000949"
     CDS             4720..5016
                     /locus_tag="ACHBKA_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9444658.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5188..5808
                     /locus_tag="ACHBKA_000950"
     CDS             5188..5808
                     /locus_tag="ACHBKA_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9444659.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5900..7066)
                     /locus_tag="ACHBKA_000951"
     CDS             complement(5900..7066)
                     /locus_tag="ACHBKA_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9444660.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7078..7431)
                     /locus_tag="ACHBKA_000952"
     CDS             complement(7078..7431)
                     /locus_tag="ACHBKA_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9444661.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7559..8581
                     /locus_tag="ACHBKA_000953"
     CDS             7559..8581
                     /locus_tag="ACHBKA_000953"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM9444662.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8600..8761
                     /locus_tag="ACHBKA_000954"
     CDS             8600..8761
                     /locus_tag="ACHBKA_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444663.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9020..9454)
                     /locus_tag="ACHBKA_000955"
     CDS             complement(9020..9454)
                     /locus_tag="ACHBKA_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444664.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9564..11423)
                     /locus_tag="ACHBKA_000956"
     CDS             complement(9564..11423)
                     /locus_tag="ACHBKA_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9444665.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11424..11564
                     /locus_tag="ACHBKA_000957"
     CDS             11424..11564
                     /locus_tag="ACHBKA_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM9444666.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11541..12701)
                     /locus_tag="ACHBKA_000958"
     CDS             complement(11541..12701)
                     /locus_tag="ACHBKA_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9444667.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13008..14432)
                     /gene="araA"
                     /locus_tag="ACHBKA_000959"
     CDS             complement(13008..14432)
                     /gene="araA"
                     /locus_tag="ACHBKA_000959"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM9444668.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14448..15146)
                     /locus_tag="ACHBKA_000960"
     CDS             complement(14448..15146)
                     /locus_tag="ACHBKA_000960"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9444669.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15103..16764)
                     /locus_tag="ACHBKA_000961"
     CDS             complement(15103..16764)
                     /locus_tag="ACHBKA_000961"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM9444670.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16982..18382
                     /locus_tag="ACHBKA_000962"
     CDS             16982..18382
                     /locus_tag="ACHBKA_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM9444671.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18634..19713)
                     /locus_tag="ACHBKA_000963"
     CDS             complement(18634..19713)
                     /locus_tag="ACHBKA_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9444672.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19799..20758)
                     /locus_tag="ACHBKA_000964"
     CDS             complement(19799..20758)
                     /locus_tag="ACHBKA_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9444673.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20770..22281)
                     /locus_tag="ACHBKA_000965"
     CDS             complement(20770..22281)
                     /locus_tag="ACHBKA_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM9444674.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22303..22866)
                     /locus_tag="ACHBKA_000966"
     CDS             complement(22303..22866)
                     /locus_tag="ACHBKA_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9444675.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22872..23735)
                     /locus_tag="ACHBKA_000967"
     CDS             complement(22872..23735)
                     /locus_tag="ACHBKA_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9444676.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23749..24645)
                     /locus_tag="ACHBKA_000968"
     CDS             complement(23749..24645)
                     /locus_tag="ACHBKA_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9444677.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24820..26109)
                     /locus_tag="ACHBKA_000969"
     CDS             complement(24820..26109)
                     /locus_tag="ACHBKA_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9444678.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26081..27598)
                     /locus_tag="ACHBKA_000970"
     CDS             complement(26081..27598)
                     /locus_tag="ACHBKA_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM9444679.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8084 a         5842 c         4788 g         8975 t
ORIGIN      
        1 ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtaaaat
       61 cagcggtttg acctgagtat tagagataca aaacggaaaa atagcttctc atattttgcc
      121 gttttttgag ctaataccga aggacagctg attgaacacc gtttattcgg aaaaagggac
      181 tgtgacacga cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa
      241 gcttcttctt cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc
      301 aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct
      361 gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg
      421 cggttcatcg ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca
      481 tcaaggatca ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc
      541 tgcccttgcg aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat
      601 ttacgaacaa aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt
      661 gcatcgtcgt tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct
      721 tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca
      781 ccatcgtaac gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt
      841 gtcgtcttcc ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc
      901 aagttaaaat cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa
      961 cgaactttgt atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct
     1021 tcttcatcca acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc
     1081 atcaaacttg caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct
     1141 tgaacatccc ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg
     1201 aacacatagc ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat
     1261 tgagctttcc aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct
     1321 ttttcagtat gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg
     1381 ttattcaata aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct
     1441 acgatcatca cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc
     1501 cagctgattg aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat
     1561 tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac
     1621 atgatatccc catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct
     1681 tccaaatcat gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc
     1741 tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc
     1801 gcgattttat caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga
     1861 gcgccagcca ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt
     1921 tggatttgga agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata
     1981 cgtttagaca tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca
     2041 ccttttacac cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga
     2101 gagggacgtg tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga
     2161 tttcttgata agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt
     2221 ttccttcatc tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa
     2281 ctaaaacggt gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg
     2341 ttttgaaatc caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta
     2401 atgcccgtgc aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt
     2461 ccacatggct atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt
     2521 ctaatgcttt ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc
     2581 ggatcgttcc agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat
     2641 tatattgagt catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt
     2701 catataatcg agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga
     2761 ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg
     2821 ctccatgata tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag
     2881 aaactgtttt aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa
     2941 tacggtcagc tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa
     3001 agctgatcaa gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag
     3061 tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga
     3121 tcaatgtcat cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg
     3181 tataatcttt attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc
     3241 gaatcactct gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct
     3301 tttgcatact tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca
     3361 ttactgggat gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta
     3421 tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa
     3481 ccatttggat ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt
     3541 tatcaaacgc atcttttgtc agactctctg attttttata gacatccgta cgcaattgct
     3601 tacccagttt ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac
     3661 tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt
     3721 gcgctacccc tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta
     3781 cttggatcac catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga
     3841 tttttagcat ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt
     3901 tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc
     3961 gatacgaatg cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca
     4021 attcatgatc attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg
     4081 catcttgttt gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag
     4141 cgacttttct tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat
     4201 tctcgccttc caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata
     4261 gatcaccatc ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg
     4321 tctcaaagta ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc
     4381 tagggatatc cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag
     4441 aaaactcgat tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa
     4501 atgtctgtgt cggcatatgg atcctccttt ctttcaagat gacacattgt catatgacgc
     4561 tgtgtcatta taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat
     4621 catccgatac attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc
     4681 ccattaaaat agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc
     4741 caagacttca ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc
     4801 ttagattaca caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca
     4861 agacaattaa aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg
     4921 cgatcagaag cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt
     4981 ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga
     5041 ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta
     5101 ttcttcaacc ttatcaatat gacttgatag ccttttcttt ttctcatagt tataacaatc
     5161 atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa aggtcatccc
     5221 ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac aagttataaa
     5281 gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga tattccggaa
     5341 atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga attcacttcc
     5401 ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga tcagttcctt
     5461 gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc aacaaaatta
     5521 aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac agaaaacggg
     5581 ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg cggtgtatac
     5641 aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt aggcatcgat
     5701 caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc agatgagttg
     5761 atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa attgagataa
     5821 atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtacc ttggatattt
     5881 tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct tcataaagag
     5941 aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct gctgcataaa
     6001 caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc agcagaacta
     6061 ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga gagatcaagc
     6121 gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc atgaatggac
     6181 ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct aagtaaggga
     6241 tcaggttcaa aaaacccgca ataatcccca acgtgacagc atactctaaa ccaatgaccg
     6301 caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt cctcttatat
     6361 aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt tgcatgcgtg
     6421 tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat agataaaata
     6481 agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg acaaaacttc
     6541 caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc gaattcatga
     6601 acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg tcccgcaaaa
     6661 tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg atgaagctca
     6721 ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt agtgcaacaa
     6781 ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt tcaaagtaat
     6841 caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc agacctacga
     6901 ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat aaaaagacga
     6961 tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt aaaaaccagc
     7021 gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt cctcctgtta
     7081 gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat gaagatagat
     7141 tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata cgtgagctac
     7201 tgtctctaaa ttcccattgg ccatcggacc gacataatcg atcgtatata cctgatcatc
     7261 aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata cttcttgaat
     7321 caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca gcatgggttc
     7381 ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca ttgcactctc
     7441 tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg tgtggaataa
     7501 ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat cagctcatat
     7561 gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa tctatacgat
     7621 cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg aagaaaatag
     7681 ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta gtgtggatgg
     7741 attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg taacagagtc
     7801 tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat acggggcgaa
     7861 ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac tgaaagcagc
     7921 agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc atgcgcatgt
     7981 ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg gtacagatcg
     8041 tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta ccttcactgc
     8101 agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat tcgcttattt
     8161 atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa caggtgcttt
     8221 tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa atggaggggc
     8281 agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag gacataactc
     8341 gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc aaatcatctc
     8401 aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt ttgtcgtgtg
     8461 tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa caggattact
     8521 ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt ttgatcgtta
     8581 gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac gggaagaact
     8641 aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag aggaatatca
     8701 gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg tagaaaacta
     8761 atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt ctatcctgat
     8821 gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa aggagtattt
     8881 actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt atcataaagg
     8941 attttgatca ctctatcata aaggtaatat tacatttata ttagataact ctagttttta
     9001 ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt tggatggcag
     9061 ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat agatcttcat
     9121 ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct acatatgaaa
     9181 gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga cgcaaagtac
     9241 tgactttctt aagcattgcc cgatattttt ttgttccttc ttcaaataac aggatgatct
     9301 cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg tttctgatca
     9361 cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg atttgatcga
     9421 tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt tctccattgt
     9481 tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa aaaatttaat
     9541 ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc taaactatat
     9601 tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat ctcttgttgc
     9661 gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag ttctcgttca
     9721 aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt tgttgtctga
     9781 ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc atcgtgagtc
     9841 gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc atgcaatgtt
     9901 tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg atccccccaa
     9961 aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg cttcagtgcc
    10021 gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc acttccatct
    10081 gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat atgtgtctgc
    10141 atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt cattacttct
    10201 ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt ataggtcatc
    10261 gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc tcttttatac
    10321 atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt taacgctgaa
    10381 acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt ggtgagttgc
    10441 gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt tgctgttcca
    10501 ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac ttcataataa
    10561 tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc tgagaaactt
    10621 tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt tttttcatcg
    10681 gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata tccttcaggt
    10741 tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc agtgttatag
    10801 tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc ttttcgcaac
    10861 aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc cacaagcagt
    10921 gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa catgtaccag
    10981 caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag catagtgccg
    11041 gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg ataaatcggt
    11101 tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat ggctacgaca
    11161 tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat aagaataatc
    11221 agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc aaatttcaac
    11281 gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact aaaaacaaca
    11341 agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg tttggtcgaa
    11401 agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata aaagaatgtt
    11461 gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa gaattaatga
    11521 aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg gacctttcgt
    11581 actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa atcagtactg
    11641 tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat atctgcgatc
    11701 gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt tttcttttga
    11761 taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg ataattgacc
    11821 gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc caaacgtccg
    11881 ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag tatacggtca
    11941 ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg ttgtttttcc
    12001 aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt gataagtaca
    12061 gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt atcttctgtc
    12121 aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa atctgcttgc
    12181 cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat ccctaagcta
    12241 tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc gatggttttg
    12301 ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc taaaatatta
    12361 ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga agataagagc
    12421 aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt attcatgacg
    12481 atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac accagctctt
    12541 gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact acgaataact
    12601 attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa cttttctatc
    12661 tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc tccttactaa
    12721 caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc ctagaagcaa
    12781 aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac aagttactgc
    12841 cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata aatggtgttc
    12901 cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca aatttcgtca
    12961 atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta tctaataacc
    13021 actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt tagatttagc
    13081 gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc ttcatggaaa
    13141 ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc agtcgttggt
    13201 tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc tgccatcgat
    13261 acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc ttctctatct
    13321 cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt cggatctact
    13381 tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta agtataatct
    13441 tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc aatcgctgca
    13501 gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg ttgtacagct
    13561 agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa agcgttatag
    13621 cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac ttttacatgt
    13681 tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc cttgtactct
    13741 tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac aagatctccg
    13801 ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt gtctccttct
    13861 gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc aaaactttca
    13921 ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc aggtcgtttc
    13981 cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa cccatattcg
    14041 cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt tttccaagga
    14101 atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag ctttgtcccg
    14161 cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc tgctacttct
    14221 tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac tgctaattct
    14281 tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac catttcttgt
    14341 gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga tcctacaaca
    14401 aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta tttgttttgt
    14461 ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat ccgttgaggc
    14521 agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca tacttcatct
    14581 agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa tggaccgtgt
    14641 ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt gaatgtttct
    14701 atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg tgtcaaaaat
    14761 cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat cgcaggaaca
    14821 tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac gattcctccg
    14881 atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga gggattgagt
    14941 ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc tgtcatctct
    15001 tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc aaaagcgctc
    15061 acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt tgcttggtat
    15121 acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca attgatgaat
    15181 cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct gatagcctgc
    15241 tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca cgtcttctgc
    15301 caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta ataacgcgat
    15361 tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct ccatagcaga
    15421 tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc ccactaaacg
    15481 atcaatttgg acattctcag atttcaatat atccattccg attctcattg caccaaaagc
    15541 actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg gtgtacgtac
    15601 aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat aaccataaga
    15661 aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata ccgtgaataa
    15721 tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa tactgaccca
    15781 agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt ctcctgaagg
    15841 tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta atacgatcat
    15901 agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccgt ttggctacac tgttcgtagc
    15961 gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg gtctcaaatt
    16021 tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt cttctcctgc
    16081 tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt tttcgattaa
    16141 ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat cgataggaaa
    16201 catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc aatggacata
    16261 acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat tcaaaataga
    16321 ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt tttccgttaa
    16381 tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta ctagtaactg
    16441 ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa tcgacccgat
    16501 tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc ggtaactgac
    16561 gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt ctagtttgtt
    16621 ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca atacagcttt
    16681 gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga catctaacat
    16741 actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc aaacctgatc
    16801 aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg tacatgtcaa
    16861 gcggttattt acaagaatac aataaaaaac acttgttttg tacgtacata agttataaaa
    16921 tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg agagaatact
    16981 tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg catttggcgg
    17041 cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt tacagcatga
    17101 ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg tcatgttagg
    17161 tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac gacgaaaaat
    17221 gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat ctgcccctca
    17281 caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg ttggcgctgc
    17341 atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa ggggaagatt
    17401 gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg ttgcagatta
    17461 tttgctaaaa gtgttgcctg aaacgatggc ttggcgcgtg atgttagggc tcgctgctgt
    17521 ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc gttttcttat
    17581 gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga caccaaaaga
    17641 agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga caaccggaac
    17701 ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg gtattggtgt
    17761 agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc cgctgatcgt
    17821 agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca tccaaggaat
    17881 cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca atcggcgaac
    17941 tttattgaaa ataggcggta gcgtgatggg actttcattc atcctaccag ctgttttagg
    18001 aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat gtatttatgt
    18061 cgcattttat tcttgcacat gggctccact gacatgggta atcataggcg aaatattccc
    18121 actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga ttggttcttt
    18181 cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa tcgttttcgg
    18241 catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg taccagaaac
    18301 tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt atcctaaacg
    18361 tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc aagaaataag
    18421 aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt ctcatgtttg
    18481 atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga gaggtggtga
    18541 aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga cgagtaaccg
    18601 taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt tcactgtttc
    18661 ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt tttcaatcgt
    18721 tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg tcaatgttgt
    18781 caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga tcgataggtc
    18841 atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag ctatttgatc
    18901 gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta ctgttttttc
    18961 agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca aaaaacgcac
    19021 attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt gaagatcgtc
    19081 gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt ctgctgccat
    19141 aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt cataagcagc
    19201 attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg ctaagtttgg
    19261 gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat aagcaatcat
    19321 tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta aaagcaatga
    19381 atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa aaatataatc
    19441 agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg ttttttcttt
    19501 cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat agccctcatt
    19561 taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata tttcttgcag
    19621 cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga tcttttgttt
    19681 tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt atttgtacgt
    19741 acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt tcttgtgctc
    19801 ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc cacgtaaagc
    19861 attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg ttcttctctg
    19921 gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta aacgagttgt
    19981 gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc gataatttat
    20041 gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg cccatggcat
    20101 agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg cgagtaagga
    20161 ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca ggtatagaaa
    20221 ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga taaagattag
    20281 aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt ccttgagaag
    20341 agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt cccttttcta
    20401 tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg cggtgatgat
    20461 gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac cattttccat
    20521 tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct tcatgagcct
    20581 gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc cttcttaatt
    20641 caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct gtatgtttat
    20701 atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc ttcatcataa
    20761 caaatttcct cacattttct ttattctaat tacattccaa gagtattttt ctatccctac
    20821 atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta ttttttctgg
    20881 ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt gctctccttg
    20941 atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg tgcgattgat
    21001 caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat aaggaacatt
    21061 ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata cttgaccttt
    21121 agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg cttgtccttt
    21181 agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac taatctttac
    21241 gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg tatcttccat
    21301 agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc ttttttccgg
    21361 ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac ttctcaaata
    21421 tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga atcgataagc
    21481 ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag tcaagtcttc
    21541 ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct cataagcttc
    21601 ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag agcttccaac
    21661 taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt catttgctaa
    21721 cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta gctcattccc
    21781 taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc ttaaatcact
    21841 ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt catcaattcc
    21901 ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga tccatttaaa
    21961 aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat caattttttt
    22021 tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg taaagtttcc
    22081 tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga catcttgtct
    22141 aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat agacgacact
    22201 tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta ccgtaaaatc
    22261 ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt catttcctat
    22321 agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa taaaaggagg
    22381 gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa tatttctcaa
    22441 aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg tgatatcttt
    22501 gttttcagaa atcacatata tccaggataa aagaaacatg gaataaataa gtaaaactac
    22561 atatccacta attgaaagta cgattgtctg ttcttctaat aattgattaa caagaaaata
    22621 aataatgatc gttaacagac taaggaacaa gttcttacca aaatgttgct ttattccttg
    22681 aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc cattgcgcca ttacataaaa
    22741 aacagcacat gtagcaggca cgatagtaat aacaggcaaa cacacgatta cccagagaat
    22801 acctaataag atgtaatcag aaaccacaat caactgtcca taaaatttag tctcaactat
    22861 tttcatcttc ctcactcttt cattccagac atagctgttc cttgaacaaa atatttctgt
    22921 gcaaaaatat aaaccactag taatggcagc attgaaataa ccgttccagc cattacaggt
    22981 ccataaaaag aaatgttctg ccctgtaaat aaagctaacc ctgctggtaa agttctcatt
    23041 tttgaactac ttgtcaatat caatggataa agcaagtcat tccaactatt catcagcgtg
    23101 aaaatcccta atgataaaag agtaggtttg ctcaatggca acatgatttt caaaaaaata
    23161 ttaaactcac ttaatccatc aatgcgtgct gcctcttcca aagaatctgg taaagtaata
    23221 aaaaaggaac gcatcatgaa gataccaaat gcagttgtca tacgaggaat gatcaaacct
    23281 gcataagtat caagcatgcc aagcaaattg acttcaataa atagaggaat catgaagact
    23341 tggaatggga tcatcattgt cagcaaaacg aaatagaaca aaactgattt tcctttgaaa
    23401 cgcatacgag caaatgcata ccccgcaaga gaatcaaaga atacagaagt gatcactgtc
    23461 cctccagcaa aaatcactgt atttttgatg taatctaata aagggatcgt tgtccaaacc
    23521 ttaatatagt tatctagggt atagctatct gcaaatattc taggcggaaa tgagataatg
    23581 tctttttcgt gtttaaaaga agaaataatc agccacaaaa aaggaaacgc aataaccaac
    23641 aatataacta gcatactaat cgttcgaatg atttttttac cactaactgc ttttcgacta
    23701 ttcacagtaa ctgtaatcga gctttctggt gccatacagg tttccctcct atttcatttg
    23761 tttttctttc cgtgacatgt acacattagt ggagatagct atagttgcaa caatgataaa
    23821 caaaaccgaa gacagtgctg atgcatagcc caattcataa ggtgctgtaa atcctcgact
    23881 gtaaatatat tgaacagctg tttctgtctt aaattgtggg ccaccagcgg ttgcaacata
    23941 tacttggtca aacacttgca tcgaaccaat cagattcgtt aaaatacaaa aacctaagga
    24001 gggaacgatc tgaggtagcg taatgttaaa aaactgctgt cgatttgttg caccatccat
    24061 ttgagcagat tcatacaaag agggagagat tccctgaata gcagtcaaga gaatcgtcat
    24121 agtaacacca aaattcttcc aaacagtcat gatggctacc gtcggcatag caagggttgg
    24181 atcgcgaaaa aattgaggga tcggcaagcc gacttgtgaa aacaagtaag gaatcgcccc
    24241 tacattcgga tcaagcaaca tagaaaaaat aatcgcaatt gacgtcaatg aacaaataac
    24301 tggaatgtaa aatgtcgaac gacaaaaacg attgaatctg gtatttttag ataatgctac
    24361 tgctgctaat aaaccaacga taatttgtgt tggcgtttcg agtacggtga aatataaaga
    24421 atgaatcaac gaattaactg ctcgttcatc actaaatata cgtgtgaaat tttcaaaact
    24481 tacaaaattt cttgatgtaa agaaaatatt caaatctgta aaactaatag caaatgttcc
    24541 taaaagcggt atgacagtaa ataataataa aactaaaaaa gatggagtta aaaacaaata
    24601 cgcagcacga tcaggacgat tccaatattt ggtgaatccg ttcatatagt ccacctcatc
    24661 tttcattttg ataaaatata caatcttggt ttaaacgaat ctgaataaga tatccgaaca
    24721 atcgctgtga tttttgcaat aacacctatc tgcaaatact aggtcgatca ttcagatatc
    24781 tgtatcttgc caacagtcta tcctgtataa agttctgtac tatttttgca gtaattgatt
    24841 gatggcatca tctgcatcgt tcatgagtgt ctcaggatct tctcctgcta ataatttttc
    24901 aatcagtgga ttgatgatat cgttattgat cgcagagatt tgggtaaaac ctggtaaata
    24961 tggctctgca tagtccattt gtttcgataa ttcagataca ataggatcat tttttacttc
    25021 ttcatctttt gctactgaat ccaagtaggc aggaaaacca ttttcgatac tccatttttt
    25081 accaatttca gttgtattcc aatatttcaa aaactcatag acagcttctt ttttattatt
    25141 atctgtactt gccggaatgg catatcctac gccatctaaa atagctgttt ttttgtctga
    25201 atcactccct tgtggtaagg tagtcacatt ataatcaatc tcgtttgctt tcaatccgtt
    25261 atttaaccat ggcccattga tttcaatggc tagttgacca gcattcatta aattgtccat
    25321 ttcggcgcca gaaatcgatt ctggaccagt cttgtctaca tggatcattt tttggatcgc
    25381 ttttaacgtt ttaaggttct caggagacgc aaattcagac ttagtataat catcgttcac
    25441 taatttaccg ccatttgcta acatccagtt atataacggt gcagttccat ccttattgaa
    25501 gacaaaacct gatacatttt tactactatc agttaatttt ggtgccattt ctgtcaattc
    25561 ttcccaagta gtaggtggtt gttcagtatc taatccagct tgtttaaaca aatcattatt
    25621 ccaatacata tacatgcctt gtacctgcat ggggacaaaa tactgtttac cttctacttc
    25681 acctaattca atagccgttt ctttaaaatc agatttatct actccatcat attcccagaa
    25741 atcgtctaat ggttgtaccg ctccgttttc tacatactgt gcaaaatctg gataatttaa
    25801 tgcaataaag tcaggtgctt ttttagaaga aatcgctgta ggaagttttt cattcaaatt
    25861 ggcccaagtc atgatatcca tttcaatttt gatgttttta tcatttgttt tattaaattc
    25921 attaacgatt tcttgcaata tttcaccatc agatgcagta aacccattcc aaaaagtcaa
    25981 agtcacttct ttatcagaat cagattctgc tgaacttccg ccaccgcaag ctcctaaaat
    26041 acctgttgca gctaccaaca aagagccaat taaccatttt ttacctttca ttttttgttc
    26101 ctccttcatc tgataaacga catctgacca cattccaaga tagcggtttc aataaacaat
    26161 tgattttccc atcttcagct tttacatttg ttgaatgact tggttttact tgttcacttt
    26221 taggaagatt agttgctttt gtatcaaatc cactcatttc agaaaaatca atcactccct
    26281 ctaatgataa ttcagacaag tgaacagtta gattcatttc ttcttctcca cggttaactg
    26341 caaatatgac aagttctttg tcatttatta cggctatact ttctaaatac ggcacttctt
    26401 caaaatcttc cgtcgcatag tttgctgaac gaatttgtgg gcttaacacc tctccttgtc
    26461 catacacagc agcttgcata aatggataat agatcgtctg tctccaagct tctccgtttg
    26521 tttctgtcct gattggtgca ataacgttta ccaattgagc aagacaagca atctttactc
    26581 tatctgcatg tttcaataaa gtgattaata gacagcctaa tagtaatgca tcttcaaaat
    26641 tatagttatc ttctagtaaa ggaggagcaa tctgccaagg cgctgcttgc tcatcttgtt
    26701 ccgctgaatg ataccaaata ttccattcat caaaggacag gttgatctgc ttcttactat
    26761 gtttcttcgc cttgactgta tcgcacatcg ctgtcacttc agaaatgaac cgatccatgt
    26821 ctaaggaacg tgcaagataa tgagaaagat catttttttg gttgccataa tattgatgca
    26881 gagacaaata atctatttga tcgtacgctt gatcaagaac tgttaactcc caatcaccaa
    26941 atgtaggcat ttgccgattc gaacttccgc ataaaaccac ctcaattgaa tcatctacga
    27001 gtttcatcgc cttagccgtt tcctctgcta accgtccata ttcttccgct gttttatgtc
    27061 caatctgcca aggaccgtcc atttcattcc ctaaacacca tgtcttaatt ccaaaagggc
    27121 tctcttgacc attcgaacgc cttaaatcac tccaataagt tcctttagga aaattacaat
    27181 attcgactaa attacgagct gcatctactc cacgagtacc cagattgaca gccatattga
    27241 tttcggcccc cacatgctga caccagttat aaaattcatg aatcccaact tcattcgttt
    27301 caattgtccg ccaagctaaa tccaaccttc taggtctatt tgctatgggc ccaatactgt
    27361 cttcccaacg aaatcctgac acaaaatttc caccaggata tctaatcatg ggaatgttca
    27421 attcttttac caagtgaatg acgtcttggc gaaatccact tttattagct gatggatgat
    27481 ctggctgata aatcccttca tatactgctc gtcccatatg ttcaataaat gaactgtaca
    27541 accgcggatc aactttgcct acacgcttga cactactaat caacattgtg gcttccataa
    27601 ctgacctcct ttaatttata catgaataat acacctcgtt gaaagcgttg tcaataatat
    27661 aaatgttaaa cttacttatt ttttatcga
//
LOCUS       DAYYOA010000024        26661 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000024 DAYYOA010000000
VERSION     DAYYOA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.024"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..834)
                     /gene="truA"
                     /locus_tag="ACHBKA_000971"
     CDS             complement(94..834)
                     /gene="truA"
                     /locus_tag="ACHBKA_000971"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9444680.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1025..1660)
                     /locus_tag="ACHBKA_000972"
     CDS             complement(1025..1660)
                     /locus_tag="ACHBKA_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9444681.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(1807..3027)
                     /gene="sstT"
                     /locus_tag="ACHBKA_000973"
     CDS             complement(1807..3027)
                     /gene="sstT"
                     /locus_tag="ACHBKA_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM9444682.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            3246..4244
                     /locus_tag="ACHBKA_000974"
     CDS             3246..4244
                     /locus_tag="ACHBKA_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444683.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4440..6275
                     /locus_tag="ACHBKA_000975"
     CDS             4440..6275
                     /locus_tag="ACHBKA_000975"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9444684.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6335..7051)
                     /locus_tag="ACHBKA_000976"
     CDS             complement(6335..7051)
                     /locus_tag="ACHBKA_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9444685.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7278..7820)
                     /locus_tag="ACHBKA_000977"
     CDS             complement(7278..7820)
                     /locus_tag="ACHBKA_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM9444686.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7825..8052)
                     /locus_tag="ACHBKA_000978"
     CDS             complement(7825..8052)
                     /locus_tag="ACHBKA_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM9444687.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8131..10011)
                     /locus_tag="ACHBKA_000979"
     CDS             complement(8131..10011)
                     /locus_tag="ACHBKA_000979"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9444688.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10200..10814)
                     /locus_tag="ACHBKA_000980"
     CDS             complement(10200..10814)
                     /locus_tag="ACHBKA_000980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9444689.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            10956..11516
                     /locus_tag="ACHBKA_000981"
     CDS             10956..11516
                     /locus_tag="ACHBKA_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9444690.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(11821..12135)
                     /locus_tag="ACHBKA_000982"
                     /pseudo
     CDS             complement(11821..12135)
                     /locus_tag="ACHBKA_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(12389..14024)
                     /locus_tag="ACHBKA_000983"
                     /pseudo
     CDS             complement(12389..14024)
                     /locus_tag="ACHBKA_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14579..15142)
                     /locus_tag="ACHBKA_000984"
     CDS             complement(14579..15142)
                     /locus_tag="ACHBKA_000984"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9444691.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(15157..16200)
                     /locus_tag="ACHBKA_000985"
     CDS             complement(15157..16200)
                     /locus_tag="ACHBKA_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444692.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(16333..16737)
                     /locus_tag="ACHBKA_000986"
     CDS             complement(16333..16737)
                     /locus_tag="ACHBKA_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9444693.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(16970..17605)
                     /locus_tag="ACHBKA_000987"
     CDS             complement(16970..17605)
                     /locus_tag="ACHBKA_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9444694.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(17871..18923)
                     /locus_tag="ACHBKA_000988"
     CDS             complement(17871..18923)
                     /locus_tag="ACHBKA_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9444695.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(18936..19874)
                     /locus_tag="ACHBKA_000989"
     CDS             complement(18936..19874)
                     /locus_tag="ACHBKA_000989"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9444696.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(20325..20975)
                     /locus_tag="ACHBKA_000990"
     CDS             complement(20325..20975)
                     /locus_tag="ACHBKA_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444697.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(21075..21584)
                     /locus_tag="ACHBKA_000991"
     CDS             complement(21075..21584)
                     /locus_tag="ACHBKA_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFM9444698.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(21785..22654)
                     /gene="scrK"
                     /locus_tag="ACHBKA_000992"
     CDS             complement(21785..22654)
                     /gene="scrK"
                     /locus_tag="ACHBKA_000992"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9444699.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(22674..23795)
                     /locus_tag="ACHBKA_000993"
     CDS             complement(22674..23795)
                     /locus_tag="ACHBKA_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9444700.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(23797..24192)
                     /locus_tag="ACHBKA_000994"
     CDS             complement(23797..24192)
                     /locus_tag="ACHBKA_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9444701.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(24211..25008)
                     /locus_tag="ACHBKA_000995"
     CDS             complement(24211..25008)
                     /locus_tag="ACHBKA_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9444702.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(25010..25798)
                     /locus_tag="ACHBKA_000996"
     CDS             complement(25010..25798)
                     /locus_tag="ACHBKA_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9444703.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(25812..26288)
                     /locus_tag="ACHBKA_000997"
     CDS             complement(25812..26288)
                     /locus_tag="ACHBKA_000997"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9444704.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(26360..>26661)
                     /locus_tag="ACHBKA_000998"
     CDS             complement(26360..>26661)
                     /locus_tag="ACHBKA_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9444705.1"
                     /translation="NPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLV
                     KQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         8379 a         5426 c         4134 g         8722 t
ORIGIN      
        1 tcttttttta tatttcttta atttgcttca tacattattt aacatcgata tttatactgt
       61 gaaacatgcg tttcatccat tatcaaagta tttttatcga taataaactt catccaaaaa
      121 cagaccttgt gcagggacgg tttctcctgc atgttgccgc actttgcttt caaatatttc
      181 atcgattgtt tctatcggca tggcacctgt gccgatttct agtaatgtcc ccatgataat
      241 ccgcaccatt ttatataaga atccgtctcc gacaaaagta aaatgaagca tttctccctt
      301 tcgttcgata gataattcat caatcgtacg cactgtggat ttttttgatt ttttcaatgc
      361 agaaaaacca atgaaatcat gtgtacccac taatttttcg caagctgcat tcagcttgtc
      421 catatccaac tcttgaggga aataaaagct gtggtttcgc tcaaatgctg aaggaatcac
      481 gttgttccaa acataatagc tgtatttttt gcccaccgca ttatatcttg aatggaagcg
      541 ctcaggcatt tcttcgattc ttttgatgac aatatcccgc ggaaggtaac gattcataaa
      601 gtcgatcatt tctgccagtc caatttctgt gtttgtttta aaatttgcta cttgtcctcg
      661 cgcatgggtg ccagcatctg ttcttcctga tccaataatt tctatctctt catttgtcat
      721 ctgatgcaat actttttcga tttttccttg aatggtttta tcgtcatctc caagtctttg
      781 ccatcctgaa tatctttttc cgtcatattc gattgtcaac ttgatgtttc tcatatttta
      841 gtggctcctc tgtcttccat ttggtcttct ctctaccttt ttaagattgt agcacaaaat
      901 atagctcctt gcttctctaa gacagaagct atacaagaaa actgtggaaa tatatatttc
      961 agaaaaacaa aaaacagcca ccggatgaag tatccgctga ctgttcaatt tgacggctca
     1021 agttttactg tttgagatgc aagatactca acctggcaat gggcacataa ttaaagaaaa
     1081 aactgattct ttggcaaaaa cctattttat tcagtaatgg ccaagaattt atagaaggaa
     1141 ttcttgccag tccataaatc gccgcaaaaa gcaaactgat cacagacagt ccaaggtata
     1201 agtggcttac gtccatttgc ccattttttc gatagatcaa gaaaagaaga actggaaaca
     1261 ataaaaaacc actgtaacct aaacctgatc cgatattatg gacccatgtc gagacattcc
     1321 acgaagattc atgggtattg atactgaaca aacctgtaaa gatacaatca cctataccat
     1381 agaacgcgat ggctgctgta actagggtag ctaacacacg agatgtacta acaaatgcag
     1441 catacaatgc aggcaatgac aggatgaaaa agatccctga aaccacagac caaataagaa
     1501 acgcttttct tacaggactg gaaacctctc caaatacact gatcacgctc ttcatttgat
     1561 ctaattcagg ataatacaat cctaatacgt acggagtcaa aaaatcactc agcacgccta
     1621 ataataaaaa gaaaaaacca aatttacgta aaaaatccat gatacgcctc caataacaat
     1681 tacctatgct tttaattgtt attataaacg actttatctt gatttaacag aaacctctat
     1741 ttacacgaac atctatataa ctcattaaaa actcgaatca ttttaaaaat actaaatatc
     1801 taacgttcaa cgttcactta atccatctaa acaatctttc aatttgattt tttccccact
     1861 tcttttacgc gcgcctagct ctcctattgc agtaaacaac agatcacttg acgaatttaa
     1921 ggcagtttcc attgaatcct ggataacacc gataataaat ccaataccga cgatttgcat
     1981 cgcgacatca ttagagatac caaacaaact acatgctaat ggaatcaata acagtgaacc
     2041 gcccgcaaca cctgaagctc cacaagcagc taaagcagac aaaatacata aaaggacttt
     2101 caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca gtcaacgtca tcaaggaaat
     2161 ggtaatcgcg gcaccgccca tattgatcgt agcacctaac gggatcgata tggaataaga
     2221 ctcttttgtc aatttcaatc tttcagccaa catcatgttt atcgggatat ttgcggcaga
     2281 acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa caaaagaaaa tcaatggata
     2341 gggattttct ttaataaaca aaaacgtgag gaatgggtac acaactaatg cgacaaacag
     2401 catcgtaccg atcaaaacaa gtaaaagctg cagatactct gccaatccag cgattccagt
     2461 cgtagcaatt gattgataaa ccaatcctaa aatccctatc ggagcaaatg caataatcac
     2521 ttgtacgact tgtgacaacg ctatagatat ctgatccacc aactcttttg tcgattctga
     2581 acgtgctcga aaagctagtc ccaacaatac agcccagaat aagacgccaa ggtaattcgc
     2641 atcaataaga gattgaacag gattttgtgt gacattcgtc aacatagtag ataaaacact
     2701 ttccaaactt tgcggtgctt tttcagatac tgtttcttgt aaagtaatag gaacagtaaa
     2761 aattgaactt acaataactg ctacaagtgc agaaagcaat gttcccacta aatataagat
     2821 caaaataggc ttcacgaatg tcttattccc aattttatgc ttggcaattg acgccatcgt
     2881 caagaaaaaa accagaatag gtgcgattgc cttcaagcaa ctgacaaaca acgttcccaa
     2941 tattgaaata aatgtccatg tggggaaaaa gataccaaac aatattccga ctaaaatacc
     3001 aacaacaata cgttgaacta acgacatatc caaaaacttc tctaacaaat gaccaccctt
     3061 tcacaatttt aacaatcatt gtatcatttt atgcaaagaa actaaaggta taattttcta
     3121 aaaattctaa caatacaaac aaccatttct tgctaatttt gtactttttc tgtctacacg
     3181 ttgttttttg agatattcta agttaggatg ttattaatgg gcaacttatc aggaaacgtt
     3241 ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt cttgaaaaaa ataaagaagg
     3301 agagttatgg cttcctgaca atctttgtcg cttgtataat gatggtttta cagcgtatat
     3361 tgatttcagc agtcactatt cctacatatg caacaaaccc tggtcgattc tacatctata
     3421 catttttaca aacttccgtt gttattggtg caaatttgat cccactttac atgggttacc
     3481 aatatcaaaa aatcgcccgt ttaaaagttt tacattattt aagtcgtttt gcctttatat
     3541 tcctaatagc cacactagtt tgcaatatct ttttttacgt acaagctggc agcttgaata
     3601 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     3661 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     3721 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     3781 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     3841 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     3901 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     3961 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     4021 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     4081 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     4141 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     4201 cagggaaggc atggctgata aatatcgggc agttttttat ttgatattta ttagcagtgt
     4261 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     4321 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     4381 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     4441 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     4501 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     4561 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     4621 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     4681 ttccttcaga tttaaaaatg ctgaccggat tgtcagaact attatcgatg gttagcccag
     4741 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     4801 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     4861 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     4921 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     4981 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     5041 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     5101 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     5161 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     5221 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     5281 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     5341 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     5401 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     5461 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     5521 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     5581 acaaagaaac cttgaaagct ttacaaagca atacggaaac gtcacaattc atccaattat
     5641 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     5701 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     5761 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     5821 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     5881 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     5941 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     6001 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     6061 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     6121 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     6181 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     6241 gtgcaacatg ggcgacacaa aaaataaaag actaacaaat aaactgagca aggggttgtg
     6301 ataaaagaca tgtcacaacc ccttgtttat ctcattaatt tgcttgtcca taaacgattt
     6361 gattaaagcc atttttcaat tcattattcg ctgtctcgtt gaagtaaaga taattatcgg
     6421 gattcccctg attttgttgt gtcaccaata cttttggctg attttgctga aacccgaata
     6481 gatagacgtg ctttttcaga tatggttctg tctctgcatc ggaatagacg gctgtgattt
     6541 gatagtctgc ttgacctgtc ccactttctg accattctgc agaaacaggc actccaccaa
     6601 tagccatctt ccactccccg ttcaaaatag cttgagggac tttaagacca tataagtcca
     6661 cactcatttg attcgtataa cttttgtatt gttgtcccat tgtttggccc catttggtca
     6721 taaatgcctc taattgtgcg gatttgtctg cattccacag tgtttgttca acttgttcgc
     6781 tgctttctga agaggaagat gaagcctcta cagaagaact tgaagtagat gtttgagttg
     6841 tttcagtcgt tttggaagac tctgacgaag tagaggtatc tgctgtttct gtacttgctg
     6901 cagttgactt gctctctgtg ctagttgttg tagtagttgc aactgttgct ttcgctgttt
     6961 cttttgttga ttcttttttc gattcattcc cgttggtaca accagctaac aaaaaagcta
     7021 gaccaattgt tactaaaatc ctttttttca attaaaaaac tcctttcgat tactcccact
     7081 tctttattct atcaaataag caatgtgatt ttattgattt ttcgtatttt ctcatcaatc
     7141 tttcaaatct tctaaaaact gagaagcacc cgctgatgtt gtctgtaata catcagcggg
     7201 tgcttctttt attttaatct tcgtgatttt ccaatttacc tcacgcttta tatttgtatt
     7261 tgaagaaaca tagactttta gtcatcatcg tcttcctcat ccaaatcttg tcggacagtt
     7321 tttccttgga gtctttgtac ttggtagatg atttctttat tgaaacttaa aagttcaaca
     7381 agcaattgtt gcaagccata tttttcttct ttttcagcta atttgattgc ccgtgtgata
     7441 aagacatttt ggaaatcaaa atcttttgca aagttttcca acattgttga ggctggtaaa
     7501 tatttgtttt gaccatgttc gtaaattttt ccataacgca tgacttcttc taaagtagac
     7561 ggaacgattt cattttcaat cactaactca tgagagattt tattgaacca tctcgcttct
     7621 tgatcagcat attctgaaaa aagggtactt aattcttgcg aagatgtacc caaaacatac
     7681 aattgtgctt ggatcaattt catttcttct aatttaaaat ttgctaatac atgccccacc
     7741 atggcgccag cagttggttt atgatggtcg atatctgctt gttttgtttc ttgaacaaat
     7801 ttttcttcca ctatattcat tgatttacgc ctcttttact tttgctgatt ggacttcata
     7861 acctaatttt tccacaactg cagctaattg ttcagggtgg acttcatctg gatcaaagtt
     7921 tgctttgatt ttgctcgcat taaacaaaac tttcgtattg cttaccccag gctgttgaga
     7981 aaccgcatgt tcgatttttt gcatacagct tggacaagct agtgtatcta attgcataat
     8041 aatttttttc atcgtagttc ttccttcctt tttcttgata gtcttattct agattacagc
     8101 ttccataaaa aaattgattc ttatcaagtt ttacgatttt tcttctttgc tttcgtgtcc
     8161 aaaatacaat aaacgcatcg cgttaaagat aacgatcaag atactagctt cgtgaacgaa
     8221 catcccacta gccatgtaaa taaacccggc aaataagccg ttgagtaaaa ataagacgac
     8281 accaatggca atcactacat tttcacgggt atttaaaaca gttttcttag ccaagcggta
     8341 ggcatgaacc aacgattcaa aattagattg catcaagaca acgtcactcg tttcgatagc
     8401 aacatccgtt cctgatccca tagcaatccc gatatcagcc gcagccaaag agggagaatc
     8461 attgattcca tctccaataa aagcaacgcg caatccttgt tcttggtatt ttttcacaaa
     8521 ttgaaccttt tcgtctggta ataattcagc atggacttca tcaatgccta acatatcagc
     8581 tacataatca gcagtcatct gattatcacc tgtcagcatc actaactgtt ttgctcctgc
     8641 ttgtttcaac ttagctaatt gttcagcgac ttctggtcga atcacatctg acacacctat
     8701 gatttgagtg atttttttgt cgattgctac aataatgacc gagctgcctg tttgttgtaa
     8761 ttgatgtaaa tcttgtactt gctttggtgt gagagaaagt ttgtgagcgg caatcaattt
     8821 ggtgttgccc gcacaaactg tatgaccatc aatctcagct gtgattcctt gtcccttgat
     8881 tgtctgatta tctttgactg ctaattgttg aaaatcaatg tctttctcct gagcataatg
     8941 gatcacggcg cgccccagag gatgatcgga taacgtttct aatcgagcca ctaaagctaa
     9001 gctgtcccct tcatcctctg tatagttctt gatagtggat acagcagtag ttcctttggt
     9061 caatgtacca gttttatcaa agacaaaagt atcgattttg gaaaaagtat ccattgcttc
     9121 accgcctttg actaagatgc cccgtttcgc tccattaccg attcctgcta cattcgaaac
     9181 aggcgcacca atgactaaag cgccaggaca accaagaacc aaaacagtga ttgctaaacg
     9241 aaaatcacga ctaatcagac caacaaccaa cgcaataagt aaaacggttg gagtataata
     9301 ctgagaaaat cgatcaatga atttttccgc atgggatttg gtatcttgtg cttcttctac
     9361 taattcgata attttggcaa aggtcgtatc atctccgacc ttagttgctt caatcgtcac
     9421 gtagccatct gctaaaatcg ttccagagaa gacttcatct ccactgattt tgacgacttg
     9481 tcgtgattca ccagtcactg cggcttcatc cgcataccct ttcccgtcaa tgactcgtcc
     9541 gtccacagga atagctccac cagtttttac taagacgata tcgccttctt ccacgtcatc
     9601 gatttccact tcttcagtcg ttccatcttc ttggacaaca ctagcagtag atggggccat
     9661 ctcggtcaaa gccttgattg actcccgcgt tttggtcaat gttttttgtt ccagatagct
     9721 accaaacaaa aacaagaatg taacgatagc tgactccttg tattctccga tcataaaggc
     9781 accaagcaca gcaatagtaa cgagtaaatc aattgagata actttgactt tcactgcctg
     9841 atacgcatgg atcataatag gtatgaccgc aataatagaa gctatggcta gagcaatgtt
     9901 atagaacaag acaaaaccag taaattttcc gataaaacca aaaataatca acacagctga
     9961 taaggcggtg atatgatttc gatgctcttg gatccacttt tgtaatttca tctttctccc
    10021 tcttttcctt tcattcttac gagactaagt atagatactt atccatcata aaaaattgac
    10081 cactatcaag tttagtcaaa aaggcggttt tcttttggta gaatacacta caaaaaatag
    10141 ttagcaagaa gactcactaa aagtgatctt gccaactatt gtcgtttcta tcgaaatttt
    10201 cattgaatga ttttcctgta cagcatacat cggagatcta tttctttatc taagattttt
    10261 atcttgctaa ttttttcctt tagtctttca aatccacgtc gttcatagaa ttgatacgta
    10321 cagccactat ctgtaaaaag gaatatttct tttcctttct ctcttttttc aaattctgtt
    10381 agcaacttgg taccgatgcc ttttgttttc aaatcaggat ttgcagctaa aaaaatgatc
    10441 tctccatctg gcgaatgctt ctttaaatat gctgcaagca tttgttcatt tgctgtctcg
    10501 taagctcctt tactttcttt agcaaatata ttttgtaaaa aatcgaatat cttaacatat
    10561 atttgctgtc caaaagaacg atgtttcttt ttttctcctt ttatctccgc cagcaaaaca
    10621 cctgccaact cgtcctctgc atacaaagca agaatttgag ttgctcgatt caactccata
    10681 tacaaaaaat atcttccgta aagattcagc agaagttcat tttccatata tctctcaaag
    10741 ttcattcctt caacagcaaa atagatagct tttttgtaat ctttcttttc taagggttta
    10801 aaagttagtt tcatcatttc gcctcctcaa cttcaatgat tgtgttatac ttacattata
    10861 atagacacag tgtctactat ctataatcaa tcatctataa gtttgtcgat taagcgacag
    10921 ctttgctttg atatgtcgga taaggagata ggtaaatgaa aaagacagat agacgcgtca
    10981 aaaaaacaga aaaagctttg gctgaaacac tatccacatt attagtaaat aaaaagattc
    11041 aagcaatcac tatacgcgaa ctaacagaaa cggcagatgt acatcgttcg actttttata
    11101 cacattataa ggatatctat gatctatatg atcaattaga aagcaacttt tttcgggatc
    11161 taaatgaaat tctctcttat gatccagccc atagctatga ggaactatac actcgcttga
    11221 ttgactatgt ttacgcaaat acttttttat atcgcttatt tatcctcaac catggtgata
    11281 tttcgtttca gaagaaagtt aaacaagtta ttgaagaaaa ctatctgaac atatggctat
    11341 atgaagacaa gaaaacgatt atcacagaag aaatgagatt ctttactacg taccacgtcc
    11401 aaggttgtct atccgttctc aatcggtggg tagaaagcaa ttacaagtac tccaaaaaag
    11461 aattattaga actcttacag aaactgaatg atcacataga gaaaatcatg ccttgaataa
    11521 aaacatacta agcttagtta agaagaaccg taaccttctc gtataacaac tgaatcttat
    11581 ttagccaaat tgtttactga agattttttg agtaaaattt ctataattcc tacttattga
    11641 aaagtagttt gtagtgctgc ttctccctct ctaagtgata tatcttgttg taccaatcaa
    11701 attcacctca tcattaataa attactttta ggctaattta ttacaactta taggttgaag
    11761 ctgtaaccaa tttatagtca tattatattt ttatcatcca gtttattgaa ttcttctcgt
    11821 ttacctcaat tcattttcaa ggggaatcag ctcatcatct aacattttac gccatgctcg
    11881 cttcatttct tgtgggtatt taaatggttt cgcttctgga tgtttggctt tatatccttc
    11941 gtaatcctta ctgttgataa ttgctttcat attaggaatt ggatcttcac ctggcatata
    12001 ttgcagatta gaagcatgta attttttctc agaaacaaac cactcattgg aaaagacatg
    12061 aatcaaatca tcacttactt gtgtaaaata gcgacgttta atgacaaaga tatcttcatt
    12121 gctatctagt gtaccttcat ttctttgtgc ttaaattcaa agataatttt ctcaataaag
    12181 atttttgatt ccagtaagat tcctttactc cttttacaac atagcgttgt cctttgtttt
    12241 gaaacatctt cttcccctct ttgccattaa aaatagccca attctctata aagaagatta
    12301 agcttcatta ttataatata aagtttaaat ttggtttatt acttactttt tcttattctc
    12361 tagtaagcta atattgaaaa aaatgtaatc agatcattat ttttatatgt tgtaacttcc
    12421 tgataagaaa actaattaaa agaaagaggt aactacataa actaattaaa aaagctaact
    12481 tcatagttgg aggataaatg gaagaaactt caatgattgt ttctccttgt gttccttcaa
    12541 tcgacactag tcctattatc aatctctctt ttttgtccat ttacttttgc gactgttccc
    12601 caataatcaa atacaggaag cgttattttt tgtcctttag attcatgtac tgtatagact
    12661 attttcgaat ccatcacttg ataaggcata gtatgccatt ccttgccaat tcttacttca
    12721 tgattctgca aatgttcttt gtattgtaat gcttccgtgg gtgcatagtc ataaagtcct
    12781 ccgggaagga gtgtcgtgta acgaataaga ttttcttgat tcattaccaa gtgatctctc
    12841 tgagtgacaa ctttatgttc atagtttgct tttgttgcag cagatacagc caacagtaat
    12901 aaaacaattg ccgttatatt aactactttt ctttgcttag ctgatgtgct cttcatgaaa
    12961 acgaacgata aaatcaacga gccaaacagt acttgaaagc ctaagattcg ccaaggaaat
    13021 tgaatcattt gaatgggtgt attttgtaat aaagaccatg gaaaaaattt ggtggataag
    13081 agccaagaga ctaccgcact aaggtaaatc actttttctt tgcgattaaa tcctttaaaa
    13141 aagaagagtg gacagatcaa agaaataata ccaatgattc ctaccgtaaa ggttgtcagg
    13201 tcattattca tcgcaccaat caaactatcc gagacattta atgcttggtg ttgtaaggtc
    13261 gttttatctg gctcattaat cgattgaacc atgatttgtc ggaacatcgg ataccaaaag
    13321 tatgctgtta aaagaatcgt cattcctact gcctttgaca actccacaat caaccggatg
    13381 ctttcttttt ttcttgtttc acttactaca aagccgccta agactagtaa gccaacggct
    13441 attacactca taaaagtgga aagaacatgt gtataaatga gtaagctcat actgactgct
    13501 aatggaattg cacatggctt tttctcataa atcagtgcgt aagcataata cataactatc
    13561 ggtataaccg taagtgcaat cgcttcagca atagcagaac gatgataaat atcaatcgta
    13621 cggtacccat aaaatgtata taaacagcta aatataaaag ccgcatcgat tctttttaaa
    13681 aattttagtc cataatgaaa catcagcatg atagtacaaa cattcaatag ccaaacatat
    13741 aagatataac taacgattaa attattactt atccaataaa aaatgactgc cggaaaaaag
    13801 gtcaagtagg gataaaaata attcactccg ctcccataat gattaaacgt cgtaaaattg
    13861 attggaccag aaaaaattga agataaccct ttgactctag caaaatggaa ggcaatatcc
    13921 cccgatgtag aaatagtatg aacatttcta aaataattga ttgaataaag tccagcaata
    13981 attgctagga gaatggacaa tattattttt ttctttactt gcattactta attatatact
    14041 cctttcatgg attgctttat ctagtataac ttaaatttta aataaatcgt tatttatttt
    14101 tactctcact taaaaaagca ctaaaattat aagagaggga ggtaatggtc tgaacaccaa
    14161 gaattgttcc aacatcgaat tgcttaacta atgagttcaa agcttaatta tgtggctctt
    14221 atatttaccg agcgtggatt gcgatctctt tagcgaaaca aacatctttc ataaaaaaaa
    14281 cagccatatc cgctttcttg gatatgacta tttactacta ctttactatc tttcattctt
    14341 cttgtctcac ttatgataag gcgctgcgta tcatgagtga atgatattag gaaaacttca
    14401 taaaataaat cttttataca tttccacttc tagatggtca ttcatgttag aagaatcaaa
    14461 ctattttttt actgtcgtaa taatattaaa acactagaac aaatcaatta aattccataa
    14521 aatataaata atagacctag catcatctgg tataaaatgg atgctaggtc cgttatattt
    14581 actcaaaatt gattctacga atagatcttc ttatcatttc tattttagta gtcatgcgtt
    14641 gttgttcttc taaaggtggt aatggtataa gtagtttaga taaatttgta ccatttacat
    14701 ttggttgccc agttccgtaa gaaatttttt ctaataactt ccaatataat ggcgagtcta
    14761 agaagcagtc tacgtattct gataataatt tctcaactaa cctaattcga atcaagtaag
    14821 aagcaaaaac agattcctca cttatttcct taaccaagaa actttttccc atagttcctc
    14881 ctgtccttgc tatcaaaatg tcattttctt ccaatcttaa atcaactagt ttagaattac
    14941 taatatcaca gtaaggcaca ctggaccaat ttacacgtcc ctcttgtata tctgtgattc
    15001 tcaaaaactt aacattgcct tgttttttcg ccgaatctgt atagccatat tgaatcttag
    15061 tagaaatact tcctaaataa acccatagtc gaacatcgct agatgatttt tcttgtcgtt
    15121 ctttatgaac aacagtgatc atcttgagtt gtccgactat tttttagttg cagcaatctt
    15181 tttgggacgc ttatttgcat gaagcaacgt tgctaagtct agtaataaaa cattggctaa
    15241 cacaggattg gctttctcgc gatacgctgc ggcgccactt tcaaacaata ctgctagacg
    15301 atcttctaca acaacgattt gttccaaata cggtggaaat ttgattcgtt ttaggatccg
    15361 gctaggagag tcccgctcgg acactaagag ttccgaggta taaggaccat aagaacgact
    15421 ggctaagaga taatggccat aaaaagccac accttgaatc ttatcagatg tttttctgct
    15481 cattgtcacg gttggttgga ttttgtcttt gcggcgaatt gctgttttca tttcagctat
    15541 cgtcggcaat agatagaaag tcaactgtcc ttcacctttt agtgtgaagt tgccagatac
    15601 cagttgatca ttgttttgag tcaaatacga tgcttgtgga atctctgaga gatcagttgt
    15661 cagccaataa gcgattggct gtcccgtctc ttcagatgta tctgctaaga tatcgatcaa
    15721 acgtaaagcg gctactcgtc cagtagcaga tcctgtgatt gtcaaccata aaagttcccg
    15781 ttttgtatca tatactaagc cccctacatg tggccgttta ggtaaaatca gcagtttgat
    15841 cggctgtcct gtttctctat ccagtacatg aataatagaa tgatgctgat ggtcatagca
    15901 gtaggcagaa ataaacacaa aatcttgaga aaaagccaac ccttgcggcg tcatccattg
    15961 acaaacctca ttttcgccgg tttccggcac caccattctt gtttctgtta atccaggaat
    16021 gttatattgt tttgcttcag ccttttccca agctgcttga acaataggat accgttttaa
    16081 ctttttttca atatcctgat gtatccgttg ctgcttccaa tgcttaaact ttaggacata
    16141 gtaaacaaga aggccaatga ccagcaacac taaaatcatt attgctaccc gctgtgtcat
    16201 ttttttgcct ccttgttcta ttcgatcaat cgtccatata ccgctattaa ttaccgtaac
    16261 tacatgtttc ttttactagc gtttctttat aggatctttc ataatctcat ttatagtaac
    16321 attaagaatc ccttattcta ctatagtcac ttccacatca atattgcctc ttgttgcttt
    16381 agagtatggg caaaatgcgt gggcttcttc tgcgattttc tctgcttggt ccttctcaag
    16441 tcctggcaaa gaaacttcta aaacgacccc gatttggtag cccccatctt ctaattgaaa
    16501 taaagatacc cgtgctttga cttcagaatc agactcaaga ttattttctt ccagcatgtg
    16561 ttgaaccgcg ccgttgaagc aagatgcgta tccagccgca aaaagttgtt ccggatttgt
    16621 cccttcacta tggattcctg ggcggtcgat cttcatgtgc atacttccgt ttggtgcctc
    16681 aacttcgcca tcacggcctc cacgattgat cattgctgtt tcgtacattt ttttcattga
    16741 gatcatcctt tctttcgtcg ttctatatta agtgtaacga gtatttttta atctgacaaa
    16801 ggtaatgctt gcattcggcg acatcagtta gacaacatcg atgatcagtg atagaagcag
    16861 tttatctata caaaaaaaga agggttctat gatatttggt gtccttactc gccttgagta
    16921 acaacaccaa atatcataga accacttaat cagcttcaca ctgctttttc tactttcgta
    16981 gttgaaaaaa acttaaacaa atcatcggca aatagttaaa gaaaaaactt aatcgttgaa
    17041 agaagcccaa ttgcttaaag aaggcaaact gactaatcga cggaatccgt gctaatccgt
    17101 aaagacctgc aattagtaaa ctaataactg ttaaaacaag ataaaaatgg ctaagcgtac
    17161 cttgaccact ttgacgataa agcagaacaa gtaacaatgg gaaaagtaga aagcctgcat
    17221 agcctagccc agaacctata ttatggatcc aagtcgatag attacaagag ctttcattcg
    17281 tattgatact gaataagcca gtaaaaatac aatcacctat cccatataaa ccaactgttg
    17341 caacaacaag tattgcaagt gtttttgatg ttccaacaaa taggtgatac aacgcaggca
    17401 acgaaagaac aaaaaaaagt ccagatacaa ctgaccagac caagaaagca cgtctgactg
    17461 gactatctac gtcaccaaac acactaatta cggtagtcat ttgattcagt ttgggataaa
    17521 aaaggccaag gatatacggt gtcgataaat cactcatcac acctaataat aaaaaataaa
    17581 aaccatattt ttgcaaaaaa atcattttct tcccctactt tttagtgatt attcgataca
    17641 tccaacaatc aaattttttc tcattttaac acatattttt attctagttg tcttttgaac
    17701 aatcaataac ataaaggtga gtttcttcta cattttaaaa aatcgaatgg cactgaagag
    17761 ttcagttgtc agcatcaatc tcctactcaa aaaggcaaaa aaacgtaagt cagatgttgt
    17821 tgacatctca cttacgcatc cattgattga aaatttctta gtaaaatcta ctagcctaaa
    17881 aatttgaaga cttcttctcg atcatacagt ttaaatactt tctcgcataa aaattgagta
    17941 attagtgccg caaaaacagg gatgactaac caacaaagaa caacaagaaa gccatttagc
    18001 cctgcatcta aagaagccaa cggaccaaca agtccgacca cgccgaaacc agcagaagca
    18061 ggcgtacctg aaatagaaaa tagagcgaca ggaatagctg taatcactgc actaactaaa
    18121 caaggaataa gaatcacagg ttttctaaat agattaggca tcatcatctt catagcgcct
    18181 aaagcaatcg caattgtcac acctgactta ttaaccttcc aagagttcac tactaaaaca
    18241 aaagtggttg cagctacgcc catcgcagcc gcaccagcag aaacaccatc tagttggatt
    18301 gccattccga ttgctactgt tgaaatagga gagataatca aaaaggcaaa ggaacaacca
    18361 atcaagatag acattaacag cggttgtaag gtagtgaagt tattaatcaa tgatccaatt
    18421 gcagaagtaa ttgctgttac aaaagggaat aaataaaaac caagtaaacc tgcacctaca
    18481 cctacaacaa tcggtgttaa cacaatcgac actgacccaa aactatttcc aatcagtaaa
    18541 atcagcccga cagcaatcga tgctgtcagc atcgtattaa taatatcgcc tgtaccagca
    18601 ccaacgtaaa cacccgtttc acccaaatca ggtgtgaatt tgactacacc agaaccaaca
    18661 aaagaagctc cggctacaac catcattctc atcggatcaa atccaaattg taaggcaatc
    18721 aaaccaccaa tgatcaaagg tgttgctagt tgaaaaataa ctgctaactg tgcgatcgtt
    18781 accgcaaagg gatacgcact aaaatatttt aaaatggcac ctaatactgc attaggaatc
    18841 aatccaataa tgattcctgt tgctgtacca gcgaggactt tattcagaaa aattttcggt
    18901 gatatttttt gtgaagtttc cattcaatct ctcctttatt ttacgttcaa actatcttct
    18961 tttgcatgga caagctcagt caagaaatca caataaggcg tcgcaacacc atatttcttc
    19021 ccttttctag aaattgcgcc attgatataa tcgatttctg ttaatcgatg gttcttaatc
    19081 aagtcttgat acattgaagg ataatgtaac ccaattgttt ccgggtcaaa acaagattca
    19141 caatgtgcaa tgacttctgg gacatctagt tcaatcttct ctactgctgc tactttggca
    19201 aactcattga caatcgtcgc caccatttta tgagcagtcg atgtttttcc tagctcagcc
    19261 atattaacgt ctaagattgt gcatagtccg ttcattgttc cgttcacaca tgctttgcga
    19321 taaatagaat agtgaatatt gtcagaaaaa tgagcattta atcctgattc agacaatttg
    19381 tctgctaatt tttttgcagc tgcttctttt ccatcaccta aattttgtaa ttctactgaa
    19441 ccacttccaa ataatttgac ctgaccagga ccttcaagac ctgccgtcca catggtattt
    19501 ccaatataga tattttccat tggtacaaat ttctcaataa tatcttcatg cccgatacca
    19561 tttaatagac ataagacctc tgtatctttt ttgattaatg attggatatc ctgcagcatt
    19621 ttttccagct gcatcgcttt ggtaaataga ataatcagat caacttgatc ttctttttct
    19681 acttcggatt gaagaacgat tggtagtttt gcttctacct ctttaccatt aaaatttgct
    19741 tgtaatccat gttctttgat ttgttggaca tgttctgccc agccatcgat taataatact
    19801 tcgttcccac tttggtgaag catcaaccca aagcgacttc ccattgcgcc agcgcctgca
    19861 attgctattt tcattgattc ttcctccttt ttctttctat tatactcatt tgagaatctt
    19921 ttcacaacct cgttttgtta ttcttctctt atttatcatg tttatatcca ccattcaagt
    19981 tattttatca gcaaatcaat aaaaactgga atagaatccc aatttcatcg taagagaaac
    20041 tagactagca ataagaaaat agaaacattc atagaaaaaa tctcactcct ctgattataa
    20101 aagagggggc attaacctcg tgtatactaa aaccttccat tcattcgatg gatgattttc
    20161 tttttgtgaa aaaatattca ttgtagtacg atgttgcgca acacagacgg atggtattgc
    20221 ttattattta ttaaaatcga acaatacact tatttaaaaa cttctttccc agattttctt
    20281 ccatttttac agttataact agtattatca tgaaaaatag ccttttattg tctatgggaa
    20341 taataattta aaattggtac ttttttatta attagttcat cgcccaataa tctctccagt
    20401 ttcttagcat ctcctgactc aaccggaatt atccaagccc agtctaaata attttttaaa
    20461 atctcatcat attttgcaaa tatttcattg ccatattccg tccttaaaat tatattttca
    20521 gtgccagaca agtgatttat attgagttga taaaaaagat ctccatctaa aaaaatatca
    20581 ttagaagaag atttccccac agtaatgacc attccatcag ctctatgtgt tacatacaaa
    20641 taattgtatg acctcatttc acctaatgaa tcaattattt cttgcccttt agctctaata
    20701 tattgatctc tattacctat ctcactctta tttaacaaaa gttttttcat gtcaaattta
    20761 atcggctgtt ttatttttgt aaattcgctt ctactactaa tccaatccga gattaatttt
    20821 tcaacatgtc ccagagtaaa ctctgattta ttcgctaaat actttttttg ttgtattaaa
    20881 gacatctttc cccgaagctc aaaattgagc ctacatcgat tctctatctc ttcaaaaagc
    20941 atcttatctt catcattctt atattcatca ttcataaaac caatccagct cctcgattaa
    21001 gataatattt taatctcata caaaaactag ctctcatcaa aacaaccatt gttttgattt
    21061 ctcatgaaat ttctttatac ttttctccga ttataaattc ctaacccctt gagaaaatta
    21121 cgcatatatt tatctccgca aggcttaaaa tttcgttggc cttcttttct caaaaatgcc
    21181 ccgatttcgc cttttgatgg atagatttct gcctcagcaa aaacgtctaa aatatcatca
    21241 gtggtcatag acaaggcgat ttttaatttt tttagcaaca cattgttgat atatttgatt
    21301 tccttgcttt tttgcggcgt gtctactatc ggcttatctt tttgcggacc acgcgccaag
    21361 agaatcaggc catttaggaa tgcgtcaaaa acttggttgt tgatggtttt ttcatacaca
    21421 ttattttctg cattatcttt gttcttatcc tgtactttgg tcagtaccgc ctgagcttct
    21481 tctttagtca gtggtacacc gcctagctca aaagctttaa tcaaatccgt atctttcaaa
    21541 tcaatcgcat aacgcaatcg caataaccta tcattgttat tcatttgttc ctccgaagta
    21601 tttagtcttt actttcttac gtactttttg tcttgctaag atttttagga tcaatgataa
    21661 aaaccgcatc acttatgata aggcgctggg tatcatgagt gaatgatatt gctgaataca
    21721 aatagtccga ccacattaag ttgatcggac ttcataagca ttttattgac taatactttt
    21781 ttgcttaaga attgattcta acatcttttg ctaaagttaa gcatcctata gttcctgcat
    21841 tatctgctaa cttaggtgtg acaatgtatt cttcgatatt tggcgtatcg acatagccgt
    21901 taattagttc taaaaaacgt ctttgtactt tctttttcag atgttcttgt tgcatgacac
    21961 caccaccaaa aattataata tctggtgaaa gcatcagggt cgtattacag gcacattgtg
    22021 caatataatc cgcctcgatt tcccagaacg gatcttctat agaaagatct tgtcccttgg
    22081 ttcccgtacg catctcgata gcgggtccag atgccaaccc ttcgagacaa tcatgatgaa
    22141 aaggacaagt accagagaaa gtatctcctg aatgtctctt aactaacgta tgccccattt
    22201 ctggatggct aaacccttca acaaaaatcc catcttgtat agctcctcca ccaattccag
    22261 ttccaacagt gtaataaaca acactggata aaccttttcc atgacctgaa acatactccc
    22321 cgtaacaagc agcattcaca tcggttgtcc aggcaattgg aatttcgaaa tgcttattca
    22381 acgttccaac aaaatcaaaa tttttccagg cagtttttgg agtagaggtg atatgtccat
    22441 aggtctttga ttcacgatgg atatcaatcg gtccaaatga ccctactcca atgctttcta
    22501 attgttcttt atagtgatta aagaaatcaa ttaccaaact cattgtttca ttcggtgtag
    22561 ttgtaggaaa actcactctt tctaaaacct ttaaatcatc attgccaatg gcacaaacaa
    22621 atttagtccc acctgcttct attgagccat acatttctta ctccaccgtc cttttatcta
    22681 ggaattcgtt cttccctgtc taatagtttt tcgaaaaact catgtggttc cttttggtac
    22741 caataggcaa cagaagaaat atcgtcttga cgctcataaa gcttgatgtc atcatttcct
    22801 agggtttgga aagtaacacg taaattttct ttgaaactaa tggggtcggg taaatgccag
    22861 cggtacaacc catgcatcgg taaagcatca tcgccaaaac catgaactgg atttggatta
    22921 ggttttccag cactaaaata atcccgggtg tgatctcttt tagattgaaa aggatagccc
    22981 ataaatagcg tattaaaatt ttgagcatat gccctttctc caaagttctt tttatggaaa
    23041 gcccaagcgc cgccaaaata atcttctgct cctgtagatg ttattgtagg gaaatcttca
    23101 tctccatcta tataaaactt aaattctcct tccccccacc agtatctttc caatgcgcac
    23161 aaagcaaagt aggttcctac ataataccca gtgcctttga ttccatcaat aagggtgtaa
    23221 tcttttcctt ttgtcgtgat gggttcacgg ttccatttag catgaaaata catcgcatca
    23281 tccggtactt cagtcaatgc ataattaaat gtataaaaaa tgtgcttaat aaactcagga
    23341 tgttcattcg ttaaagtaat cttagctttt tttcgaaatg gcatctcaaa atagcaattc
    23401 atccctgatg ttggattcac tacgattggt aaggaattca cttcgcatct tgtcccaaac
    23461 ccattacaaa aaaaatctcc taatggactt tctacagaag gagtggattc atcatcccaa
    23521 taaatacgca aaattaaatc acgtaagacg aaactcccag cgattgtttt gtctgtcact
    23581 gtcatccaaa aatgacgtaa aattccaggg ccttccattt ctgctaaagt aattgtctct
    23641 ccagcattta tcggaacaga aggtgaccct tttctcccaa ttcctagatg actcgctgtc
    23701 attgccgctt tccctttttc accattttta ttttctggtg aaatagttct actttcctct
    23761 cccttgtgga gccatatatt atttaaacaa ttcattttat tcgtcctcta tcctttcttc
    23821 acttttttga ttattcatat agatgatttg ttgttgtgcc tccgcaataa ttgctccaat
    23881 atctaaatta tcatttccta actgcaaagc gattgacatt gccaagggaa gattcattcc
    23941 tgcaattacg tgcactctgt cagaaatata ggtaaaaaac ttttgattta cacttcctcc
    24001 agccatgtca gttaaaacaa ttatttcgtc tgtagtatcc actaactctt taatttgttc
    24061 atctatatca tcaatttctt cgacatatgc acataagcta ctaatttgat tatgcatacc
    24121 tgttaaaaac tctagtgtct ctttaaaacc ctcagctagt attgaatggc tagcaatgat
    24181 caatcttctc ataattcaaa tccttttcct ttacgctaat attccaaatg cagccataac
    24241 catcgcaaaa acaataacga gtagaatcaa aatggtcata ctgatttttt tcttactgag
    24301 tagataatat gctgcagcag taataattac tggaattaat gttggtaaaa ttttgtccaa
    24361 cattggttgg attttcatac taacttcacc aaaagtgaat ttaagtggcg ttgaaacact
    24421 gataactgaa gcaattaatg atccaactac tgttagcccc aatactgaag cagcttcagt
    24481 taaataatta atcttttcac cgtattcagt aacaatcttc attccctgct tataaccaaa
    24541 ttcaaagcct ctcattcgga aaaagaagat taccatgatt aaaatgaacc ataaaaagat
    24601 tcctacagga ttattttcta aagccatata agccgcaatt gatcccatga tggttgggat
    24661 catcgtcatc aaaagagagt ccccaatacc agcaattgga cccatcgtac caactttaaa
    24721 gtcattaatc gcatcttttg cttctaatcc ttgcttttcc tccattgcaa cacaagcacc
    24781 taaaataatg gctgccagcc aaggctgtgt attgtaatac tgaaagtgat tatttaaagc
    24841 ttcgatgtat tcatcatcat ctttgtataa ttttcttaaa ataggtgaca atgcgtaagt
    24901 cacagatgca ccttgctgtg tttgataatt aaaggtacaa actgacatca accaacgata
    24961 gttcgctttc ctaagatcct ttttcgttac ttgatatttt tcgctcattt tattcatcct
    25021 ccccaataaa cccaacatca gaagctcccg cagaagcagt gatttgtgca tctttttcag
    25081 ctctatgata cacattaaat gctaaagcga atcctatgat cgccgctcca ataataggta
    25141 ccttcaaaaa agcggctaag aaaaatccaa caagtaaata actaatgtat cgacctgtag
    25201 gcataaagcg taacaacatc gcaataccaa cagcaggcaa catttttccg gctaatgtta
    25261 gcccatccat aaaccaatta ggtaaaacat ccaaaacgta attaacagca gactgtccta
    25321 aagtaacggc aataaatact ggaattgctg cagataaacc aaagaaaatt ggccccaacc
    25381 acaaaacact attcattttc ttaaattctc gtttattcgc atattcctgt gatttgcgaa
    25441 caacaaaacc atttaagatt ttgacaatta catctagttg cacccctaac atacctacag
    25501 gtagtcctat tgctaatccg gcttctactc caagctggct accaaatgcc gttgcaataa
    25561 ttgcgcacgt tccatagtcc ggtacagatg agccaccaaa accggcaact cctaatgaca
    25621 tcaattgaac tgtaccgcca actatcaaac ctgtttttat atcacccatc aaaattcctg
    25681 tgaataatcc ccaaaaaact gcgtaattta caaagatcat cggaatccca taataatcaa
    25741 catgtttaat aaatgctaat atagtcagca taataatttg aaaaatagta aaatgcatta
    25801 tccttactcc tttatcctaa ttttgattga atttcctgtg ccttatccgc cggaacaaat
    25861 tgtgtaacaa tttctacacc agaatccaac aattttttat acgtgctgat attttcatct
    25921 gatgcatagg ctctagaaga tagctgtaat gcattatctt taacaacagt accgccaaca
    25981 attagtcggt caattctaat accgttatct actaaatgaa ggattgtttc cggctctttc
    26041 acgatgacaa atactttttc ctgatcgtat ttcccctttt taaaattcgc aatcgctgtt
    26101 tcttcagtga taattgatac agccattcca gcaggtctag ctaaacgcat acttgatttt
    26161 tggatgtcgt tatttgcgac agaatcatcg atcaccatca ctctgtttgc tttagacatt
    26221 ggtgcccatt gcgtgacaat aatcccatgc aataaacggt tatcaactct cgacataaca
    26281 atactcataa atacttcctc ctttatcttt cattttctta caagcaatta ccatgccaaa
    26341 acaaaaagaa cttgataaat caagctcttt ttacataatc ataaacgtat ttaacctcat
    26401 gaatcgggat gtcgacacta taataatgtt caacgacact gaaagattgc ttaaaatgtg
    26461 tctgaaattt ttgaaattcc tcactcgttt cattgaaata aatttcatct tccaaagtat
    26521 tttgttttac taatcgttca attaagcaac taatgtgaac atataatccg aaacacgtat
    26581 tattatcaaa tgaaattcct agatcatttt gtaagatgta tatagcatct gaaacccgtt
    26641 ctaataattt agtcggattt a
//
LOCUS       DAYYOA010000025        25194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000025 DAYYOA010000000
VERSION     DAYYOA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.025"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHBKA_000999"
     CDS             99..746
                     /locus_tag="ACHBKA_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9444706.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHBKA_001000"
     CDS             1139..3784
                     /locus_tag="ACHBKA_001000"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM9444707.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHBKA_001001"
     CDS             4094..5407
                     /locus_tag="ACHBKA_001001"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM9444708.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHBKA_001002"
     CDS             5397..6050
                     /locus_tag="ACHBKA_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9444709.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="ACHBKA_001003"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="ACHBKA_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="ACHBKA_001004"
     CDS             6787..7872
                     /locus_tag="ACHBKA_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9444710.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="ACHBKA_001005"
     CDS             7869..9761
                     /locus_tag="ACHBKA_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM9444711.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="ACHBKA_001006"
     CDS             9768..10145
                     /locus_tag="ACHBKA_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM9444712.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="ACHBKA_001007"
     CDS             10299..11081
                     /locus_tag="ACHBKA_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM9444713.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="ACHBKA_001008"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="ACHBKA_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFM9444714.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="ACHBKA_001009"
     CDS             complement(11996..12055)
                     /locus_tag="ACHBKA_001009"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFM9444715.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="ACHBKA_001010"
     CDS             12447..13109
                     /locus_tag="ACHBKA_001010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9444716.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="ACHBKA_001011"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="ACHBKA_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            13637..13837
                     /locus_tag="ACHBKA_001012"
     CDS             13637..13837
                     /locus_tag="ACHBKA_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9444717.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="ACHBKA_001013"
     CDS             complement(14091..15770)
                     /locus_tag="ACHBKA_001013"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9444718.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="ACHBKA_001014"
     CDS             complement(15772..15984)
                     /locus_tag="ACHBKA_001014"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM9444719.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="ACHBKA_001015"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="ACHBKA_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM9444720.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="ACHBKA_001016"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="ACHBKA_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM9444721.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="ACHBKA_001017"
     CDS             19959..20156
                     /locus_tag="ACHBKA_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444722.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="ACHBKA_001018"
     CDS             20309..20794
                     /locus_tag="ACHBKA_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM9444723.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="ACHBKA_001019"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="ACHBKA_001019"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM9444724.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="ACHBKA_001020"
     CDS             21671..22198
                     /locus_tag="ACHBKA_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444725.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="ACHBKA_001021"
     CDS             22289..23026
                     /locus_tag="ACHBKA_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9444726.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="ACHBKA_001022"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="ACHBKA_001022"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM9444727.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="ACHBKA_001023"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="ACHBKA_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM9444728.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8390 a         4103 c         5297 g         7404 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DAYYOA010000026        24628 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000026 DAYYOA010000000
VERSION     DAYYOA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.026"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACHBKA_001024"
     CDS             complement(59..187)
                     /locus_tag="ACHBKA_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9444729.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHBKA_001025"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHBKA_001025"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9444730.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACHBKA_001026"
     CDS             complement(1674..2729)
                     /locus_tag="ACHBKA_001026"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9444731.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHBKA_001027"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHBKA_001027"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM9444732.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHBKA_001028"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHBKA_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM9444733.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHBKA_001029"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHBKA_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM9444734.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHBKA_001030"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHBKA_001030"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM9444735.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHBKA_001031"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHBKA_001031"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM9444736.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACHBKA_001032"
     CDS             10491..12050
                     /locus_tag="ACHBKA_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM9444737.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACHBKA_001033"
     CDS             complement(12161..14092)
                     /locus_tag="ACHBKA_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9444738.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHBKA_001034"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHBKA_001034"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9444739.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACHBKA_001035"
     CDS             complement(15021..15773)
                     /locus_tag="ACHBKA_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9444740.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACHBKA_001036"
     CDS             complement(15932..16141)
                     /locus_tag="ACHBKA_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444741.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACHBKA_001037"
     CDS             complement(16152..17480)
                     /locus_tag="ACHBKA_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444742.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACHBKA_001038"
     CDS             17616..17771
                     /locus_tag="ACHBKA_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM9444743.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHBKA_001039"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHBKA_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM9444744.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHBKA_001040"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHBKA_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM9444745.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHBKA_001041"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHBKA_001041"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM9444746.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACHBKA_001042"
     CDS             complement(21455..22387)
                     /locus_tag="ACHBKA_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444747.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACHBKA_001043"
     CDS             complement(22544..23203)
                     /locus_tag="ACHBKA_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9444748.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHBKA_001044"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHBKA_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9444749.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACHBKA_001045"
     CDS             complement(24244..24534)
                     /locus_tag="ACHBKA_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444750.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
BASE COUNT         6540 a         5331 c         4339 g         8418 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggac
//
LOCUS       DAYYOA010000027        24575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000027 DAYYOA010000000
VERSION     DAYYOA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.027"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACHBKA_001046"
     CDS             complement(152..550)
                     /locus_tag="ACHBKA_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM9444751.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACHBKA_001047"
     CDS             complement(547..1320)
                     /locus_tag="ACHBKA_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9444752.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACHBKA_001048"
     CDS             complement(1317..2000)
                     /locus_tag="ACHBKA_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9444753.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACHBKA_001049"
     CDS             complement(2167..3054)
                     /locus_tag="ACHBKA_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9444754.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACHBKA_001050"
     CDS             complement(3051..3920)
                     /locus_tag="ACHBKA_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9444755.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACHBKA_001051"
     CDS             complement(4057..5343)
                     /locus_tag="ACHBKA_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9444756.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACHBKA_001052"
     CDS             5616..6194
                     /locus_tag="ACHBKA_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9444757.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACHBKA_001053"
     CDS             6194..7123
                     /locus_tag="ACHBKA_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444758.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACHBKA_001054"
     CDS             7120..8856
                     /locus_tag="ACHBKA_001054"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9444759.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHBKA_001055"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHBKA_001055"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM9444760.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACHBKA_001056"
     CDS             complement(9752..10261)
                     /locus_tag="ACHBKA_001056"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM9444761.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHBKA_001057"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHBKA_001057"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM9444762.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACHBKA_001058"
     CDS             complement(11562..12653)
                     /locus_tag="ACHBKA_001058"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM9444763.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHBKA_001059"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHBKA_001059"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM9444764.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHBKA_001060"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHBKA_001060"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM9444765.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHBKA_001061"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHBKA_001061"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM9444766.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="ACHBKA_001062"
     CDS             complement(16616..17590)
                     /locus_tag="ACHBKA_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM9444767.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="ACHBKA_001063"
     CDS             complement(17571..19454)
                     /locus_tag="ACHBKA_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM9444768.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="ACHBKA_001064"
     CDS             complement(19472..21697)
                     /locus_tag="ACHBKA_001064"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9444769.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="ACHBKA_001065"
     CDS             21812..22846
                     /locus_tag="ACHBKA_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9444770.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="ACHBKA_001066"
     CDS             complement(22913..23926)
                     /locus_tag="ACHBKA_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9444771.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="ACHBKA_001067"
     CDS             complement(24004..24192)
                     /locus_tag="ACHBKA_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM9444772.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7062 a         5341 c         4397 g         7775 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattatt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DAYYOA010000028        24458 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000028 DAYYOA010000000
VERSION     DAYYOA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.028"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..911)
                     /locus_tag="ACHBKA_001068"
     CDS             complement(63..911)
                     /locus_tag="ACHBKA_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9444773.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="ACHBKA_001069"
     CDS             complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="ACHBKA_001069"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM9444774.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3412..3591)
                     /locus_tag="ACHBKA_001070"
     CDS             complement(3412..3591)
                     /locus_tag="ACHBKA_001070"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM9444775.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3638..5461)
                     /locus_tag="ACHBKA_001071"
     CDS             complement(3638..5461)
                     /locus_tag="ACHBKA_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9444776.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5739..8018
                     /gene="helD"
                     /locus_tag="ACHBKA_001072"
     CDS             5739..8018
                     /gene="helD"
                     /locus_tag="ACHBKA_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM9444777.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8111..8758)
                     /locus_tag="ACHBKA_001073"
     CDS             complement(8111..8758)
                     /locus_tag="ACHBKA_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444778.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8937..9656)
                     /locus_tag="ACHBKA_001074"
     CDS             complement(8937..9656)
                     /locus_tag="ACHBKA_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9444779.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9787..10938)
                     /locus_tag="ACHBKA_001075"
     CDS             complement(9787..10938)
                     /locus_tag="ACHBKA_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM9444780.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11155..11895)
                     /locus_tag="ACHBKA_001076"
     CDS             complement(11155..11895)
                     /locus_tag="ACHBKA_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9444781.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="ACHBKA_001077"
     CDS             complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="ACHBKA_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM9444782.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="ACHBKA_001078"
     CDS             complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="ACHBKA_001078"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM9444783.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12856..13506)
                     /locus_tag="ACHBKA_001079"
     CDS             complement(12856..13506)
                     /locus_tag="ACHBKA_001079"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM9444784.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="ACHBKA_001080"
     CDS             complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="ACHBKA_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM9444785.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="ACHBKA_001081"
     CDS             complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="ACHBKA_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM9444786.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14949..15476)
                     /locus_tag="ACHBKA_001082"
     CDS             complement(14949..15476)
                     /locus_tag="ACHBKA_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM9444787.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15656..16441)
                     /locus_tag="ACHBKA_001083"
     CDS             complement(15656..16441)
                     /locus_tag="ACHBKA_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM9444788.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16434..17303)
                     /gene="accD"
                     /locus_tag="ACHBKA_001084"
     CDS             complement(16434..17303)
                     /gene="accD"
                     /locus_tag="ACHBKA_001084"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM9444789.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17304..18683)
                     /locus_tag="ACHBKA_001085"
     CDS             complement(17304..18683)
                     /locus_tag="ACHBKA_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM9444790.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="ACHBKA_001086"
     CDS             complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="ACHBKA_001086"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9444791.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19135..19611)
                     /gene="accB"
                     /locus_tag="ACHBKA_001087"
     CDS             complement(19135..19611)
                     /gene="accB"
                     /locus_tag="ACHBKA_001087"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM9444792.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="ACHBKA_001088"
     CDS             complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="ACHBKA_001088"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM9444793.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="ACHBKA_001089"
     CDS             complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="ACHBKA_001089"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM9444794.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="ACHBKA_001090"
     CDS             complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="ACHBKA_001090"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9444795.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22641..22865)
                     /locus_tag="ACHBKA_001091"
     CDS             complement(22641..22865)
                     /locus_tag="ACHBKA_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9444796.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22918..23880)
                     /locus_tag="ACHBKA_001092"
     CDS             complement(22918..23880)
                     /locus_tag="ACHBKA_001092"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM9444797.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23890..24330)
                     /locus_tag="ACHBKA_001093"
     CDS             complement(23890..24330)
                     /locus_tag="ACHBKA_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9444798.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6693 a         5438 c         4255 g         8072 t
ORIGIN      
        1 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
       61 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
      121 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
      181 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
      241 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
      301 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
      361 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
      421 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
      481 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
      541 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
      601 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
      661 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
      721 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
      781 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
      841 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
      901 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
      961 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     1021 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     1081 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     1141 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     1201 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     1261 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     1321 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     1381 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     1441 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     1501 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     1561 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     1621 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     1681 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     1741 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     1801 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     1861 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     1921 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     1981 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     2041 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     2101 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     2161 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     2221 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     2281 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     2341 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     2401 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     2461 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     2521 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     2581 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     2641 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     2701 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     2761 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     2821 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     2881 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     2941 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     3001 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     3061 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     3121 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     3181 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     3241 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     3301 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     3361 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     3421 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     3481 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     3541 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     3601 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     3661 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     3721 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     3781 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     3841 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     3901 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     3961 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     4021 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     4081 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     4141 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     4201 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     4261 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     4321 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     4381 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     4441 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     4501 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     4561 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     4621 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     4681 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     4741 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     4801 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     4861 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     4921 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     4981 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     5041 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     5101 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     5161 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     5221 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     5281 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     5341 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     5401 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     5461 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     5521 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     5581 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     5641 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     5701 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     5761 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     5821 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     5881 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     5941 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     6001 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     6061 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     6121 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     6181 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     6241 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     6301 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     6361 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     6421 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     6481 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     6541 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     6601 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     6661 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     6721 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     6781 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     6841 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     6901 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     6961 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     7021 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     7081 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     7141 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     7201 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     7261 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     7321 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     7381 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     7441 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
     7501 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
     7561 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
     7621 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
     7681 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
     7741 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
     7801 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
     7861 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
     7921 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
     7981 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
     8041 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
     8101 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
     8161 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
     8221 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
     8281 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
     8341 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
     8401 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
     8461 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
     8521 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
     8581 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
     8641 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
     8701 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
     8761 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
     8821 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
     8881 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
     8941 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
     9001 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
     9061 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
     9121 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
     9181 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
     9241 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
     9301 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
     9361 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
     9421 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
     9481 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
     9541 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
     9601 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
     9661 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
     9721 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
     9781 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
     9841 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
     9901 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
     9961 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    10021 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    10081 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    10141 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    10201 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    10261 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    10321 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    10381 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    10441 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    10501 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    10561 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    10621 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    10681 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    10741 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    10801 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    10861 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    10921 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    10981 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    11041 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    11101 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    11161 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    11221 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    11281 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    11341 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    11401 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    11461 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    11521 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    11581 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    11641 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    11701 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    11761 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    11821 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    11881 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    11941 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    12001 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    12061 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    12121 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    12181 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    12241 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    12301 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    12361 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    12421 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    12481 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    12541 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    12601 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    12661 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    12721 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    12781 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    12841 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    12901 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    12961 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    13021 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    13081 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    13141 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    13201 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    13261 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    13321 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    13381 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    13441 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    13501 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    13561 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    13621 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    13681 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    13741 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    13801 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    13861 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    13921 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    13981 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    14041 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    14101 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    14161 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    14221 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    14281 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    14341 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    14401 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    14461 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    14521 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    14581 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    14641 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    14701 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    14761 caaaaagtcg tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa
    14821 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    14881 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    14941 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    15001 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    15061 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    15121 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    15181 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    15241 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    15301 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    15361 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    15421 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    15481 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    15541 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    15601 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    15661 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    15721 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    15781 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    15841 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    15901 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    15961 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    16021 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    16081 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    16141 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    16201 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    16261 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    16321 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    16381 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    16441 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    16501 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    16561 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    16621 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    16681 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    16741 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    16801 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    16861 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    16921 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    16981 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    17041 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    17101 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    17161 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    17221 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    17281 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    17341 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    17401 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    17461 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    17521 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    17581 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    17641 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    17701 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    17761 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    17821 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    17881 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    17941 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    18001 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    18061 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    18121 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    18181 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    18241 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    18301 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    18361 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    18421 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    18481 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    18541 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    18601 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    18661 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    18721 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    18781 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    18841 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    18901 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    18961 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    19021 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    19081 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    19141 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    19201 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    19261 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    19321 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    19381 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac
    19441 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    19501 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    19561 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    19621 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    19681 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    19741 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    19801 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    19861 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    19921 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    19981 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    20041 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    20101 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    20161 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    20221 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    20281 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    20341 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    20401 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    20461 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    20521 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    20581 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    20641 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    20701 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    20761 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    20821 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    20881 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    20941 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    21001 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    21061 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    21121 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    21181 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    21241 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    21301 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    21361 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    21421 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    21481 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    21541 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    21601 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    21661 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    21721 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    21781 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    21841 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    21901 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    21961 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    22021 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    22081 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    22141 tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt
    22201 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    22261 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    22321 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    22381 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    22441 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    22501 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    22561 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    22621 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    22681 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    22741 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    22801 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    22861 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    22921 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    22981 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    23041 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    23101 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    23161 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    23221 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    23281 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    23341 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    23401 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    23461 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    23521 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    23581 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    23641 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    23701 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    23761 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    23821 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    23881 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    23941 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    24001 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    24061 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    24121 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    24181 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    24241 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    24301 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    24361 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc
    24421 atttttttac tatatgagct ttttttaaga ttttactc
//
LOCUS       DAYYOA010000029        24263 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000029 DAYYOA010000000
VERSION     DAYYOA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.029"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACHBKA_001094"
     CDS             202..1077
                     /locus_tag="ACHBKA_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM9444799.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACHBKA_001095"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACHBKA_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ACHBKA_001096"
     CDS             complement(1642..2973)
                     /locus_tag="ACHBKA_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9444800.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ACHBKA_001097"
     CDS             complement(3122..3835)
                     /locus_tag="ACHBKA_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9444801.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ACHBKA_001098"
     CDS             4010..5434
                     /locus_tag="ACHBKA_001098"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9444802.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ACHBKA_001099"
     CDS             5733..6440
                     /locus_tag="ACHBKA_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9444803.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ACHBKA_001100"
     CDS             6427..6750
                     /locus_tag="ACHBKA_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9444804.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ACHBKA_001101"
     CDS             complement(6839..7876)
                     /locus_tag="ACHBKA_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9444805.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ACHBKA_001102"
     CDS             complement(8197..9180)
                     /locus_tag="ACHBKA_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM9444806.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ACHBKA_001103"
     CDS             complement(9595..10365)
                     /locus_tag="ACHBKA_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444807.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ACHBKA_001104"
     CDS             complement(10358..11293)
                     /locus_tag="ACHBKA_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444808.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ACHBKA_001105"
     CDS             complement(11609..13258)
                     /locus_tag="ACHBKA_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9444809.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ACHBKA_001106"
     CDS             complement(13375..15978)
                     /locus_tag="ACHBKA_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9444810.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ACHBKA_001107"
     CDS             complement(15990..17039)
                     /locus_tag="ACHBKA_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9444811.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ACHBKA_001108"
     CDS             complement(17065..17379)
                     /locus_tag="ACHBKA_001108"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM9444812.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ACHBKA_001109"
     CDS             complement(17395..17841)
                     /locus_tag="ACHBKA_001109"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM9444813.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ACHBKA_001110"
     CDS             complement(17842..19764)
                     /locus_tag="ACHBKA_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9444814.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ACHBKA_001111"
     CDS             complement(19976..20872)
                     /locus_tag="ACHBKA_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9444815.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ACHBKA_001112"
     CDS             complement(20865..21545)
                     /locus_tag="ACHBKA_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9444816.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ACHBKA_001113"
     CDS             complement(21546..22328)
                     /locus_tag="ACHBKA_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9444817.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ACHBKA_001114"
     CDS             complement(22321..23238)
                     /locus_tag="ACHBKA_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9444818.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23656)
                     /locus_tag="ACHBKA_001115"
     CDS             complement(23402..23656)
                     /locus_tag="ACHBKA_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444819.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         7369 a         4921 c         3991 g         7982 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaa
//
LOCUS       DAYYOA010000030        23830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000030 DAYYOA010000000
VERSION     DAYYOA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.030"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACHBKA_001116"
     CDS             complement(54..788)
                     /locus_tag="ACHBKA_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9444820.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACHBKA_001117"
     CDS             1115..1525
                     /locus_tag="ACHBKA_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM9444821.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACHBKA_001118"
     CDS             1518..2219
                     /locus_tag="ACHBKA_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM9444822.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACHBKA_001119"
     CDS             2219..3805
                     /locus_tag="ACHBKA_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444823.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACHBKA_001120"
     CDS             3824..4045
                     /locus_tag="ACHBKA_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM9444824.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACHBKA_001121"
     CDS             4042..4803
                     /locus_tag="ACHBKA_001121"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM9444825.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACHBKA_001122"
     CDS             5183..5692
                     /locus_tag="ACHBKA_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9444826.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACHBKA_001123"
     CDS             complement(5767..6306)
                     /locus_tag="ACHBKA_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM9444827.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHBKA_001124"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHBKA_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM9444828.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACHBKA_001125"
     CDS             7319..8170
                     /locus_tag="ACHBKA_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM9444829.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACHBKA_001126"
     CDS             8198..8833
                     /locus_tag="ACHBKA_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM9444830.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACHBKA_001127"
     CDS             8853..9626
                     /locus_tag="ACHBKA_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9444831.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACHBKA_001128"
     CDS             10034..10831
                     /locus_tag="ACHBKA_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM9444832.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACHBKA_001129"
     CDS             10809..11222
                     /locus_tag="ACHBKA_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9444833.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACHBKA_001130"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACHBKA_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9444834.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACHBKA_001131"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACHBKA_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM9444835.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACHBKA_001132"
     CDS             15999..16559
                     /locus_tag="ACHBKA_001132"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM9444836.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHBKA_001133"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHBKA_001133"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM9444837.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACHBKA_001134"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACHBKA_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM9444838.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACHBKA_001135"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACHBKA_001135"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM9444839.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACHBKA_001136"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACHBKA_001136"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM9444840.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACHBKA_001137"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACHBKA_001137"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM9444841.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACHBKA_001138"
     CDS             21906..22484
                     /locus_tag="ACHBKA_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9444842.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACHBKA_001139"
     CDS             22536..23018
                     /locus_tag="ACHBKA_001139"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM9444843.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACHBKA_001140"
     CDS             23135..23509
                     /locus_tag="ACHBKA_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444844.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6649 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DAYYOA010000031        23723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000031 DAYYOA010000000
VERSION     DAYYOA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.031"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(129..1154)
                     /locus_tag="ACHBKA_001141"
     CDS             complement(129..1154)
                     /locus_tag="ACHBKA_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9444845.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1429..3114
                     /locus_tag="ACHBKA_001142"
     CDS             1429..3114
                     /locus_tag="ACHBKA_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9444846.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3128..4411
                     /locus_tag="ACHBKA_001143"
     CDS             3128..4411
                     /locus_tag="ACHBKA_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9444847.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4481..5350
                     /locus_tag="ACHBKA_001144"
     CDS             4481..5350
                     /locus_tag="ACHBKA_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9444848.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5347..6183
                     /locus_tag="ACHBKA_001145"
     CDS             5347..6183
                     /locus_tag="ACHBKA_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9444849.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6209..7276
                     /locus_tag="ACHBKA_001146"
     CDS             6209..7276
                     /locus_tag="ACHBKA_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM9444850.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7273..8100
                     /locus_tag="ACHBKA_001147"
     CDS             7273..8100
                     /locus_tag="ACHBKA_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9444851.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8145..9170
                     /locus_tag="ACHBKA_001148"
     CDS             8145..9170
                     /locus_tag="ACHBKA_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9444852.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9167..11350
                     /locus_tag="ACHBKA_001149"
     CDS             9167..11350
                     /locus_tag="ACHBKA_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM9444853.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11350..12480
                     /locus_tag="ACHBKA_001150"
     CDS             11350..12480
                     /locus_tag="ACHBKA_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9444854.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12477..13154
                     /gene="pgmB"
                     /locus_tag="ACHBKA_001151"
     CDS             12477..13154
                     /gene="pgmB"
                     /locus_tag="ACHBKA_001151"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9444855.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13238..13765
                     /gene="msrA"
                     /locus_tag="ACHBKA_001152"
     CDS             13238..13765
                     /gene="msrA"
                     /locus_tag="ACHBKA_001152"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9444856.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13966..14652)
                     /locus_tag="ACHBKA_001153"
     CDS             complement(13966..14652)
                     /locus_tag="ACHBKA_001153"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM9444857.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14732..16270
                     /locus_tag="ACHBKA_001154"
     CDS             14732..16270
                     /locus_tag="ACHBKA_001154"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM9444858.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16390..16857
                     /locus_tag="ACHBKA_001155"
     CDS             16390..16857
                     /locus_tag="ACHBKA_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM9444859.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17391..17819
                     /gene="rplM"
                     /locus_tag="ACHBKA_001156"
     CDS             17391..17819
                     /gene="rplM"
                     /locus_tag="ACHBKA_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM9444860.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17835..18227
                     /gene="rpsI"
                     /locus_tag="ACHBKA_001157"
     CDS             17835..18227
                     /gene="rpsI"
                     /locus_tag="ACHBKA_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9444861.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18315..19541)
                     /locus_tag="ACHBKA_001158"
     CDS             complement(18315..19541)
                     /locus_tag="ACHBKA_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9444862.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(19613..19915)
                     /locus_tag="ACHBKA_001159"
     CDS             complement(19613..19915)
                     /locus_tag="ACHBKA_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444863.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            20463..21182
                     /locus_tag="ACHBKA_001160"
     CDS             20463..21182
                     /locus_tag="ACHBKA_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9444864.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21184..22026
                     /locus_tag="ACHBKA_001161"
     CDS             21184..22026
                     /locus_tag="ACHBKA_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM9444865.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            22023..22973
                     /locus_tag="ACHBKA_001162"
     CDS             22023..22973
                     /locus_tag="ACHBKA_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM9444866.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            22988..23221
                     /locus_tag="ACHBKA_001163"
     CDS             22988..23221
                     /locus_tag="ACHBKA_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9444867.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            23552..>23723
                     /locus_tag="ACHBKA_001164"
     CDS             23552..>23723
                     /locus_tag="ACHBKA_001164"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444868.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINM"
BASE COUNT         7763 a         4080 c         4884 g         6996 t
ORIGIN      
        1 tctaaaacag atggaaaata agaaaaattc gctaaaatac cgggaggggg taacgaggga
       61 tgagtgattg aaaaaagaac agagtaactg acttatcctc agtgtttctg ttcttttata
      121 attctgttct acatttcttg ttgttggatc gaatctgtga gagtaatctc ggttgaaaga
      181 atgattcttt tttcttcagg gtattggtgg tggattagtt ggaataaaag ttctgtggct
      241 ttgattccta actgatttgt cggaatgtct atccgagtca actgtggcga aaatagggag
      301 gacaataagg gatcactgaa tcctgcgatc gaaagatcat caggtatctg ctttccagat
      361 tgttctgcca ctttgtaaat actcaatgat tttaatgcat cggtagaaaa aatcgcagtt
      421 ggtctttctc gtagcttcat taaatcattc attgctgcca tgctatcttc taagcgaaag
      481 ccgccgtcta taatccattc attacggata tctaggtggt gatcaaataa gcattgacgg
      541 taaccattga gacgatcatt ggatacataa acttctgtcg gagcatggag acagccgata
      601 tgacggtgtc cattcttgat cagtgtttcc accaaatcgt aactatcctg gaaattatcc
      661 gtgtccactg agtagatgtt cgtgtagtcg tttccgactt ttccgattac cacaatagga
      721 atattatatt gatccaattc aaaaagaagt gtttcatcca tcgtagagct taaaataata
      781 attcctcgga tcattttttc ttggatcttt ttcgcgcatt tttctagctc ttcttctggt
      841 gtttttgccg tttgtagaat caagtcgaag ttattttgtt cagaaacggc agaaatggat
      901 tgaataacat ctgaaaaaaa aggattgcca taggtcgtat gagaagtacg atgagataat
      961 gctagaatcg cgtcaaaacc atttcctgac agtcctcttg caattttgct cggttcataa
     1021 ttcaattctt tgattgcttg aagtacactt gcttttgctt tatcagagat atttggctgg
     1081 ttatttaata ctctggagac agttgatttt gagacattag cttttttcgc aatgtcataa
     1141 atagtaggtt tcaattttat atgcacctcc tttttgggag cgttttagaa taattccata
     1201 gtacaataga cattcttttt tgtctatgat tttgaaaatt taggaatagc ttgcatgatc
     1261 atgagagacc atattttttt aagaagctat taattattga tacataagct ttcttatttg
     1321 tatttttgtg tatatcaaaa ttatttaaaa aaatgctatt gacagtaagc gttttccata
     1381 atatgatcaa atcgttggga acgctcccaa aaaagaaagg ggaatccgat gtatacaaaa
     1441 cttttacaag cgatttatcg cgactcagat ttgtctgaga aaaccgcctc tatgattgaa
     1501 caaaaagtca gtgtttatca gcagaagaaa ggccaggcag gcaaactgca ttgggacgaa
     1561 gaagatattt tcttgattac ttatggggat caattttttg aagagcagca accaactttg
     1621 cgtacattta agaaatttta tcaaaagtat ttggcagata catttagagt tgtacatttc
     1681 ttgccatttt tcccatatac atctgatgat ggtttctccg ttgtaaatta tcaggcaatc
     1741 aacgaccgtt tgggcacatg ggaagatata gaagaaatga gaagcgaaac acggttaatg
     1801 ttcgactttg tctgcaatca tatgtctgcc gaaagtactt ggtttcagga atatttaaac
     1861 ttgaatgata cgtacaaaga tttttttatt gatttgccac tggatacaga tacaagcagc
     1921 gtgactagac caaggaccag cccgttactt acaacgttca ttgacaaaaa cgggaaagaa
     1981 aaacacgtat ggacgacttt tagcgaagat caagtagact tgaattttaa aaatccagaa
     2041 gttttattga aaatgatcga tattcttttg ttttacttag aaaaaggcgc ttcatttatt
     2101 cgcttggacg ctgtgggatt tttatggaaa gaactaggaa cttcttgtat acacctgcca
     2161 gaaacgcata aaatcatcca attattccgt gcaatcgctg atgaagcagc tccaggaacg
     2221 gtattgatca ctgagacaaa tgtgccacac aaagataata tcagttattt tgggaatggc
     2281 gagaatgaag cacagatggt ctatcagttt tcgctaccac ctttgaccct tcaagcgatt
     2341 cgttcgggta atacaagtaa gttgaaagaa tggttaaaaa ccatcgattt cgcatcgaaa
     2401 aaaacaactt tttttaattt tttagcctcc catgacggta tcggattgaa tccggtacga
     2461 ggtattctgc cggaaaaaac gatccttgaa ttagtggaac agctgaagaa agagggtgcc
     2521 cgagtaaact acaagaaaaa cccagatgga acgaccagtc catatgagat aaacgctacg
     2581 tatctagatg cgttaagttc tccaacagat acgacggaat ggagaagcaa acgcttttta
     2641 gctgctcatt catttttggt aacgttacca ggcgtgccgg caatttatat tcaaagtgtg
     2701 ctgggaagtc gaaatgatta caaaggcgta gaaaaaacag gagagaatcg aaaaatcaat
     2761 cggcaaaaat ttttgttagc agatattgag aaagagttgc agacagatcc attaagaaca
     2821 gcagtatatg gtgaattgac ccagatgcta tctattcgaa aaaaagaaaa agccttccat
     2881 ccgaatagtt tgatttactt actggactca gcagattctt ttatcagttt tattcgcgaa
     2941 gcgaaggaag agaaagtctt agttatccat aatttttcca atcaatcagc aggttatctt
     3001 ttaccaaaag gagattacct agacctgttt tctggacaaa ttatccattg cagtcaagaa
     3061 cgaaagttga tattccagcc gtatgaatat atgtggctga aaataataaa ataagaggtg
     3121 gaaaaaaatg aaaaaaatta ttgttactac ctgtctgcta gctactggat ttcttcttac
     3181 tgcttgtggt gaaagtcaaa gctctaacga atcacaacag acaacgattc aattcatgca
     3241 ctcttctgtc gaacaggaac gattaagtgt gatcaatgaa ctggttgctg attttgaaaa
     3301 agaaaatcca gacatcaaaa tcaagcaagt gccggtagaa gaatcagcgt tcaatacaaa
     3361 aatcgttacg ttagctcgtt ccggtaaatt accagaagta atggaagtca gtcaggattt
     3421 tgctaaagtg atggataaag atgaactgat tgatcagacg gcagtccaaa acgttatcga
     3481 ggaagctgga gaagataatt attatgacgg agccaaaaac cttgttcgtt cagaagatgg
     3541 aaaaagctat attgctgcac caattagcgg ctgggttcaa ggtatttggt atgacaaaga
     3601 aaaattatca gaagctggtt tttcagaacc agaaaattgg gatgatattt taaaaattgc
     3661 tcaacatttc acagataaag agaataaaca atatggaatt gctatgccta ctgctgaagg
     3721 aacaatgagt gagcaagcgt tctctcaatt tgctttgtca aatggagcaa atgttttgga
     3781 tgatgaaggc gaggtaacga tcgatacgga aaagatgaga gaagcattgt cgttttatca
     3841 aaacttatcc caatacacga tgccaggctc taatgatgtg acagaaatca aagatgcttt
     3901 catgaatgga acagcaccaa tggcaatcta ttcaacttac attttgcctt ctgtttatga
     3961 agcggggaac tcagaaaata tcggttttgc tatcccaaca caaaaagatc aagctgtcta
     4021 tggcacagta tctggattga cgatttcttc tggtctagac gagaaacaaa aagaggctgc
     4081 tgaagcattc acagctttct tatctcaacc gaaaaacatg gaaaagtggg tcttgatgtc
     4141 tcctggtggt gctcaaccag tgaataaaca agtagtcgaa ctggatggtt acaaagaaaa
     4201 tgaagtcatc aagtcttttg gcgagctgcc atctgagatt gctagtgctt ttgatgaagt
     4261 acaagtcttt ggactagtcg gggaaaagaa tttcacgaaa atgggagata tcacaagctc
     4321 aggtgtgatc ggaaaagcag tgaaccaagt aactgtcgga aacgaagatg tcgatcaagc
     4381 tttagctgac gcacaaaaaa atacgaaata acaagaagtt ctgccaatgg tgttcgaaaa
     4441 aagtagatag ctattggcag attatcaggg ggaggtttca atgaatcgtc caaggaaaca
     4501 ttctgactta aaattagcca tgggactttt agcaccaagc atgcttttat tagctgtttt
     4561 agtgctgatt ccaatgcttt ccaatattca gatcagtttt ttcgatatgc cgctgaatcc
     4621 taactcagac gccgttttta tcggattaag caattatctt gaaattttaa gtgatagtga
     4681 attttatcgt tctttaggtt taacagttgc ttacacattc ttagttgtag caggcagtac
     4741 agctttagga ttagccgtag ctattttgct taaccgcaag ttcagattta ggaaaacggt
     4801 gcgttcactg attttattaa gttatgttac gccatctatt tctttgattt ttgcttggaa
     4861 atatatgttc aataatagtt atggaatcgt caatgatctg ttagtagata ggttgcagct
     4921 tttcaacgaa gcaccgctgt ggttcgacca gccagttgct agcttcttta tggtcgtttt
     4981 gtttgctatc tggagatact atccgtatgc gtttatttca tttttggcta ttttgcaaac
     5041 agtcgacaaa acgctttatg aagcagctga aatggacggt gcaaatgctt ggcagaagtt
     5101 caagatcgtg acactgccgg caatcaaacc agtactagca actgtgattg ttttacgatc
     5161 tatctggatg ttctatatgt ttacagatgt ctatctactg acgaataaag tcaatatctt
     5221 aggcgtttat ttgtatcaga cagcctttgc gttcaatgat cttggaaaag cagcagctat
     5281 atcgattcta ttgttcatca ttattgcagc ggtcgtcttg ctctcacgaa agaaggtgga
     5341 cttgaaatga aaaataaaaa gagcaggaaa atcttatttt acgcgacgat tttgttgcta
     5401 ttagctgtca atttgtttcc atttttgatc atgctgatga catcattcaa ggattcacaa
     5461 gaagcaattg caacgacaca aactattttt ccgaaagaat ggacttttga acattatcag
     5521 gatattttca atgctgagat ttttccattc atcatgtatt tcaaaaatag tttagtgatc
     5581 tccttgatag cctccgtaac tgcagtagtg attgggatca gcggggcata cgctttgtca
     5641 aaactacgct tcaaagctcg ctcgaaaatc aatgctagct tttacaccgt ttatatgttt
     5701 tccggtattt tgatcgttgt tccattattt aaaatcattt ccggtctagg tttgtatgat
     5761 accaatacag cactgattat cacgatggtc gttcaaacac ttcctactgc gatattcatg
     5821 ctaaaaagtt attttgacac gattccgact gatattgaag aagcagcaat gatcgatggt
     5881 cttagccgga tccagatcat tattcggatc attataccat tggctatttc tggaatcatt
     5941 tctgtcttcg tttattgctt tatggtttcg tggaatgatt atttatttgc ttcgattttc
     6001 ctgtcgacct ccgaaaaatt tactttacca atcggtttga acacattatt cagtacacca
     6061 gactacattt ggggacgaat gatggccgct tcattggtga ccgctttacc agtcatcatt
     6121 atgtatgcgt tatctgaacg atttattaaa ggtgatttag tcgaaggcgg cgtaaaagga
     6181 taaaaagaaa agaaagagga aagaaaagat gaaaaaatta gttgcattag aaccaagaat
     6241 agccggattc aaagattacg aagaccgtga gatcgaaaag aacgaagtac gtatccgagt
     6301 aattttcgct actccaaaac caggaacaga aattgtcgac tttagaggat taagtccatt
     6361 tatcgatgaa gaattttcag aagaatggcg cctattcttg ccaagagaag aaggaagcca
     6421 acgcggtgtg gtatttggcg aatttcaatt aggaaatatg attgtcggta cgatcgaaga
     6481 aacgggcagt ttggtgacag agtatcaaaa aggagagtta gtctgcactt atggacccat
     6541 tcgtgaaacg ctgattacaa acggtgttga taattataaa ctacgtaaac ttgatcggtt
     6601 ggatcaatgg aaaaatgcag tatgttacga tcctgctcaa ttcgctttag gtggtatccg
     6661 ggaagcacat gttcgagcag gtgatgctgt agcaatcttc ggtttaggcg ctatcgggca
     6721 aattgcgatt caattagcta aaaaagcagg tgctactacc atcgttggga tcgatcccat
     6781 agcgcagcgt agagaattag cagaaaaaca cggtgccgat tacacgctag atcctatgaa
     6841 agcagatgtc ggttttcgtc taaaggaatt gacagataaa cgtggagtag attcagttat
     6901 tgaaacgagt ggcagtgctg ccgcattaca agcttcttta atagggatcg gctatggcgg
     6961 gatcatttct tatgttgcct ttgctaaacc ttttccagct gggtttaatc ttggtcgcga
     7021 agcacatttc aataatgcaa aaatcgtctt tgctcgagca tctagtgaac caaacccaga
     7081 ttacccaaga tggaaccgta aacgaatcga ggacacatgt tgggaattat tgatgaacgg
     7141 ttacttggat tgtgaagaaa tcattgatcc agttgtgcca tttgcagaaa gcgcagaagc
     7201 ttacatgcgt tatgtagacc agcacccaga agaaagtatc aaaatgggcg ttcaagtccg
     7261 aggaggagaa caatgaaatt cggtacgcaa gatcaagcat ttttcccaag agacatttta
     7321 gaaaaattta gatttatcaa agactgtgga tttgaagaat acgaaatcga cggtaagttg
     7381 ttaatagaaa atatcgacac agtcaaacag gcaatcaaag aaacagggat cccagtagtg
     7441 acagcttgta acggctacga tgggtggatc ggtgacttta tcgaagaacg gcgtcttaat
     7501 ggtgtacgtc aaattgctga gattttgcgt gcactaagcg aggttggagg caaaggaatc
     7561 gttatcccag cagcttgggg aatgttcact tatcgattac cgccgatggt ttcaccccga
     7621 agcaaagaag gagactataa agcagtcagc gaatcacttc gttatttaga taaagtagcc
     7681 gaagaaacag gtacggttat ttatttggag cctttgaacc gataccaaga ccatatgatc
     7741 aatttgttag cggatgcgcg tcattacatt gactctaatc aattgaaaca tgtgcagatc
     7801 atcgctgatt tttatcatat gaatattgaa gaagatgata tttcaaaagc attgactacg
     7861 caaaaagagt atgtgggtca tatccattta gcagataatc atcgctacca accaggatct
     7921 ggcgcaattg attttactcg ccattttcaa acgctacttc aaaatgagta tgaaggttat
     7981 atggtatatg aatgtcgttt gcgaggagaa aatgaagtga aaatttatca ggaatcattg
     8041 caatacttga aaaactgtct ggaaacagcg cgtcatttaa atgatttaga gaaagaatag
     8101 agaaagaata acaataagta aataagcaaa ggagagagaa ataaatgaag aaagtagcaa
     8161 tcataggcgc tggacaagta gcagaaaaag tacatgctgc ttattatcaa aaaagatcag
     8221 atgtcttaga attggccgct gtggttgatc cagtcagcca gcgagcagaa gacttttgcc
     8281 gacgtacagg attttctcat gcgtatacat cagttacaca aatgctgaat gagatttctc
     8341 ccgatatcgt gagtgtctgt acacccaatc gctttcactt cgaacatgta ttggctgccc
     8401 tcgaagctaa tgcttcagtg atgtgcgaga aaccaccagc tatgacagct gatcaagcaa
     8461 gaaagatggc agagacagcc gataaaaacg ggcagatttt ggcttatgga ttccagcatc
     8521 gtttttctga cgaagcaaca atgatcaaga atcatcttga tgttctcggg gatatttatt
     8581 acgtagaagc gaatgcgctt cgtcggtctg gtgttccagg ctggggaacg tttatcgaca
     8641 aagaacttca aggaggcggg cctttgatcg attatggcat tcacatgctt gatacagcga
     8701 tgtatattac tggatttcct aaagtaaaaa gcgttcgggc gtacagcttc caaaaaattg
     8761 gtacgaaaaa aaattcaggg aaattcggga aatgggatcc aagcaaattc acagtagaag
     8821 atgcaatttt tggcacactg gaattagaaa acggcaagat catccaactg aatacttcgt
     8881 ttgctttgaa tatcagagaa gacaaactga atgtggcttt ttgtggcgat caggcaggtg
     8941 ctaatctgta tccaggcgaa ctattcgaag atgtagatgg aaagctggtt tccttacaaa
     9001 agaccacgcc agttaatgac ccacatgaac taagtatggc tcattttgtg aatcgggcgc
     9061 tcggtgacca gactcagatg attgcagatg gctggcaagg atatgtcatc cagaatctgg
     9121 tggaaaattt atatcgttcg gcagaaacag gagaagttat taccttatga aagtagaaga
     9181 aaaacagttt gatttagagt atatcaataa gtatgcgagt cagatgacca ttggaaatgg
     9241 attttttggt atcagaggaa gccaggagga agactatcgg aatcaagtaa ggggaatgtt
     9301 tgcagctggt gtatataacc gacctttagg agcagaatca gccgagttag tgaatctccc
     9361 agatgtcatc agatgggaaa taaaaatcga cggtgaacat ttttctcttg agacgatgaa
     9421 agttcttgca tacgagcggg cactagattt agaaatggga gagcttaaac gctatgcgct
     9481 cattgaaact gctcaaggaa aaaaagtgga agtcattact tgtcgtgttg ctgatcaaaa
     9541 taatctccat caggcagccc tgaaagtggt catcaagccg ttgaccgaag actgccaagt
     9601 agatgtgaaa acgggtatcg atggacagca aaccaatttt ggtacacagc agttggttga
     9661 aaaagaactg cgagtatttg gtgacgattt gatcagtgca tcctaccaga caacagaaag
     9721 taaaattgat attgggattg cagctaaatt caataaagca ggtgtattcc aagcaaaaaa
     9781 tcgccggatc accgcttcct tttcagaaaa gatcagaaaa aatacgtcct ttacgctgga
     9841 gaagatcatc gtgctctcta cctctcttca gcaaacagac ccgtttaaga aaagtattgc
     9901 ccaagcccag aaaagcgtta gttatacaga aattttagag cagtcgatgt cttattggac
     9961 agatttttgg catcagcatc ggataacgat ccaaggcgat tcttctttcg atcagttagc
    10021 gatggacttc gctgcttacc atttacagat tatgactccc cgcaatgatc caagtgttag
    10081 tgtaggggcg aagggactaa ctggcgaagg ttataaaggg catgttttct gggatacgga
    10141 aatttttatc ctgcctttct ttttatatca gtttcctgaa gtagcaaaga atttgctgct
    10201 gtaccgttat gatcgattag aaggcgcacg gcaaaaagca agagattatc actatcaagg
    10261 tgcacttttc ccatgggaaa gcgcacacac tggtcaggaa gagacaccca aatttgctgc
    10321 attgaatatc aaaaccggaa caagacaaga agttgcttca gctgccgcag aacatcatat
    10381 cagtgcagac cttgcttatg ccgttcaaga gtatgtccaa gcaacagagg atcaatcgtt
    10441 tttagaaaca aagggaaaaa cgctcattca tgaaacagca gctttttggc tgagcagagc
    10501 tgtaaaagtc gatgatcatt tagagataca cagagtcatt ggtccagatg aatacacaga
    10561 atatatcgac aataatgcat atacgaacta tctggcgcat tataatgtgg ccttagctgt
    10621 taaatatcaa gctggcgatc cgcttttttt gaaagaatgt caaaattttt tagataagtt
    10681 atatttgcca gtgcctaaca aagaacagct gattccgcaa gatgacacgt atctcagtaa
    10741 gccagttatt gatttgacca cttacaaaga acgtcctggc agccagtcta ttttacttga
    10801 ttatgcccgt agcgaagtaa atgaactcca agtcagcaag caagcagatc ttgtcatgct
    10861 gttatactta ttacctgagt atttttctca accaatcgtt tctaagaatt tgcattacta
    10921 tgaaaacaaa acaatccacg actcatcgtt gagtaaagcg attcacgcca tagaggcttg
    10981 ccgtatccaa gaaatggaat gggcgtatca tttattccaa gaagcgtgcc tgattgattt
    11041 aggaccagat ccgcattcat cagatgacgg aattcatgct gcttcattag gagcactatg
    11101 gctagcagtt atatttggtt tttcaggtat tacagtaacg aatggaactc tttctatttc
    11161 tccaagactt ccttcagcat gggaaaaaat ttcttttcct tttatttgga aaaatcacag
    11221 gttgctattt gttttgacaa aaaaacaaat agtaataaaa aaagagacga aagaaacatt
    11281 acctattttt gtcaatggag accaatacca gctggaaaaa gaactgatct tggaacggag
    11341 gggaaaataa tgcgtattct gattgcgcca gattctttca aagaaagtct gacagcctta
    11401 gaggcagcac aagccattca atcaggtttt caagaagttt ttgttcaggc tgaatatgat
    11461 ctgctaccta taggcgatgg aggagaagga acggtttcat cattagcaga cagtttaagg
    11521 ttgaagcggc aaacagcagt cgttactaat gcatttggca aaaacaaaga aatccagtac
    11581 gcttcagacg gtcaaactgc gatttttgaa atggcagaaa tcgtcggatt gaaagatgtc
    11641 ccactaaacc aacaacagcc acttaggtta tccacgcgag gagtaggaga actgatacga
    11701 caattgttgc aacaaggtgt tcgacacatc atcatcggcg ttggcggaag tagtaccaac
    11761 gatggcggca tcggcatggc agaaggtcta ggttatgctt tttacgatca agataatcaa
    11821 aaagtagaag cactgggaga aaatctaggt aaaattgcta cagttgataa aacgaacgtg
    11881 cctattgagc tgattaaagc caaaatcact gtcattactg atgttactaa tcttttatgc
    11941 ggaaaagaag gcgcttcttt tgttttcgga ccacaaaaag gcttgaaaga aaatgaatta
    12001 gcagtggctg accaagccat gcatgatttt tatatgagat ttgcccctca agtaatcgag
    12061 gcatcgggca gtagtgctgg tggtggaatg ggtgcaggac tgttagcatt tgcttcaggg
    12121 aaaatagtct ctggcatcga ctacgtattg gatttactgg agttcgataa acgggtacag
    12181 caagcagacc tcgtaatcgt aggcgaagga cgtctagatg gacaaagtct aaaaggaaaa
    12241 gcgccagttg ggattgctag aagagtacca gagggcgttc ctgtgatagc tatttgcgga
    12301 agtgttggta aaggaagcga aaaagcagca gatgttggca tttcggccgt ttttccgatc
    12361 cttcctgcag ctgtgccttt acaagaagct ttacagcaag ccactgataa tcttcatcgc
    12421 acagctaaaa acatcgcatc tttacttcaa gtcgttcatt ttcaaggagg taatcgatga
    12481 aaatcaaagc agcactattc gatttagatg gcgttcttgt agacacagct cgttatcatt
    12541 acgaagcgtg gctggtttta gctaaccagc tgtccattcc attcactgag aaagaaaacg
    12601 agcaactgaa aggaatatcc agaaccgagt cactagagcg attgctgtca tttggaaaaa
    12661 tggaacagaa attctcagaa aaagagaaat cagcctttgc tgagcaaaaa aacaatctat
    12721 atcttcaagc gattcaaaaa atggatgaaa catccgtttt gcctggtgca atagctgttc
    12781 ttgagtactt gaaaaaaaca aacatcaaga tcggattggg atcagcaagc aaaaatgcac
    12841 gcctcatttt agaaaaaacg aatttgacaa gttattttga tgtccttatt gacggcacgc
    12901 aagtatcaaa agcaaaacct gatccagaag tttttctcaa aggagcacag caattgaatg
    12961 taccgccaaa tgcatgcttg gtcattgaag attcagaagc tggttgtcaa gcagctttgg
    13021 ctggtaacat gcatgtattg ggaataggtg aaaacataaa tcttccttct gctgaatatg
    13081 tgattcctga tttaaccgtt tttgatcaag tacgttcgtt ttggcattta tcagaagccg
    13141 tatcacgtat ataatagttt ccgttttttc tgtcacaagc aaaacgttat cctccttttg
    13201 gaaatcaagg tatgatgaga aaaaaggggg attttatatg acaaaaaaag cagttttcgc
    13261 aggcggatgc ttttggtgta tggtcaaacc atttgaagag cagccaggga tcatttctgt
    13321 gacatccggt tatactggcg ggcatgtacc aaatccaact tatgaacaag taacaacagg
    13381 cacaacaggg catacagaag cagtagagat cgaatatgat ccagaagtta tttcttatga
    13441 tcagttggtg gaaatctatt ggcagcaaac cgatccgact gacgcattag gacagttcgc
    13501 tgatcgcggt gattcttatc gtccagtgat ctactataat gatgaagaag aacgaaatat
    13561 tgcagagcgt tcaaaacaag cattgcaaga cagcgggcga ttcgataagc caatcgtgac
    13621 aaaaatcgaa ccaagaagcg aattttatcc tgcagaagat taccatcagg attattacaa
    13681 gaaaaacaag ctccattaca atctttatcg agaaggttct ggacgtgcag gtttccttcg
    13741 tcgacattgg ggcgtcaaga aataagaagc cgaaaaagct acttatggaa caccgtatat
    13801 cagaggttgt aaaaccagca ttttgagcta atactgaggg tcagctgatt gaacaccatt
    13861 tagtggagag gttgtgaacc tgtcgcctag ctccaagtca caaggatttg gtaagagaac
    13921 actatatatc agaggttgtg acaagtcgtt tgtcacaacc ttttttcaat acctaagatg
    13981 aaggacaatc agcaacgcac tgctcaaaaa aggaatgaaa ggcagctttt ccaacttttt
    14041 tttatgacag ataaagtaaa cagaaaaaac ggatattcca aacagactag caagaaacaa
    14101 gatctgaagc aactcttcta gagacaagcc accactccaa gaaagcatca acagagtatc
    14161 gccagctccg agataggatt gaccatagcg aaggcacaca ccgcctacga taaacgttaa
    14221 cagcacactt tgccattgga agacgccggc ctgcaaccaa aaaatccata aaattgccca
    14281 agaacagaat agcgtgtgag aatggaccat gaagtaaaat aaatccatca acgaaaatag
    14341 caaagccgaa agcatccata tgaacgtcga aaaatttctt gcggaagaaa aagtaaagaa
    14401 ccaggcaaaa agaccaccac ataaggcttc tgccaacaaa tagtagaagg gcaaccgaca
    14461 cctgcaatag cggcagcgaa atcgttggac gatgatcgag agaatcggaa tcaattcata
    14521 ccaagaaagc gaacgacagc atttgacaca atgagatcta ggataaataa tagagtgacc
    14581 taacggaatc cgctgggcgg ttaaacataa aaatgatccg atacagcaac caatgataaa
    14641 ataaagtaac acgccatctc ccccttttca aaaccaagat acgcaagctt aaactaaatt
    14701 tttgtacata cataaaattt gaggtgaaaa aatgaacgtt tcaatcggat tagatatcgg
    14761 aaccacacaa accaaagcag ttgctttcac agatgaagcc caagaagcag catccgctta
    14821 ttttcgctac cctttgatcc aagaaacaca agggatggca gaacaagatc aggaattgat
    14881 ctttcaaggt gtctgcaccg ttataaaaga agtaattaaa cagttgcctt cagacacaat
    14941 cattcaattt gtctccttct cctctgcgat gcatggcctg atattgttgg atgaagagaa
    15001 aacaccgtta tctcgaatga tcacttgggc agataaccga gcagcggacg aagcagaaaa
    15061 actgaaatat accgtgcttg gcaaagaatt ttatcggcag actgggacgc cgattcatcc
    15121 aatgacacca ttgaacaaaa tcaagtggct gcaaaacagc caacctgaat tattcaaaaa
    15181 aactgcttat tttgtcggta tcaaagacta tatcttttat agattgtttg gcgaactgat
    15241 cagcgactac ggaacagctt caggaactgg atattttgat atccatgaat tccaatgggc
    15301 agaagaagtg cttgactact tgaacattcg aatagagcaa ttgccgcaag tctttccttc
    15361 cacctatcaa gtaacaggtc taccatcaaa aacagcagca gaactaggtt tgccaaatga
    15421 tatatcattt gttttgggag gggcagacgg gccgctttct aatctagggt taggtgcttt
    15481 tggtgatacg atcgccgctt tgaccgtagg aacgagcgga gcacttcgtt ttattactac
    15541 tcagcctcag ttccatccag aaatggagac gttttgttat gttctggatc agaaacattg
    15601 ggtagtggga ggagcgacta gcaatggtgc tggcatcttt gattgggcag ggcatacatt
    15661 tttacaggaa gtcgttcaat ctgctaaaga aaaaggagaa aatccatatg atgcactgct
    15721 ttcggctatc gcttttgtgc cagcgggtgc caatggtctg atttttcatc cctatttact
    15781 tggtgagcga gcaccgcttt gggacgcaga tgcgtttggc agttttatcg gtttacagcg
    15841 gcaacataca gaaaaagaaa tgatgaaggc gattctagaa gggatctgtc tcaaccttta
    15901 tcggatttta aaaggagttt gccaggaaaa tcagctcaca gagatccgtg ctacaggcgg
    15961 gtttgctcaa tctccgattt ttcgtcagat gatggctgat gtactgggtt acccgcttgt
    16021 tttccctact gtcacagaag catccgcatt aggagctgtg attttaggct ggagaagcat
    16081 cggaaagatc caaagtatca gtgaagcaca ggaattgata gaattgaaag agcaagttgg
    16141 agtacaagaa actgatcata aaaaatatca gaaaatctat ccattatttg tttcgacaca
    16201 aaagcattta gcacagactt atcagcaatt tgctcaactg cgagaagaat tagctgattc
    16261 tgaaaaatga ttggctttac ggataaaaaa gcgaactgaa aatcgttctc aggtaaatga
    16321 ttttgttgta cttttcattg aaagtctact attattttaa gtgtaagaaa atgttaggag
    16381 gcaagaaaga tgaaattcca acaaaccaaa gaagtattaa atcaattagt tgcagatcta
    16441 agccaaatgt ctgttgttgt tcaccaaaca cattggtaca tgagaggacc aggtttcctt
    16501 acattgcacc caatgatgga tgagttcatg gatgatttga acgctcagtt agatgaagta
    16561 tccgaacgct tgatcacatt agacggttca ccttattcta cattgcgtga atttgcagat
    16621 aacacaaaaa tccctgatga aattggccgc tgggatcgta ctattccaga acgtttggaa
    16681 acacttgttg caggctatcg ttatctagca gatctttacc aaaaaggaat cgaagtttct
    16741 ggagaagaag gcgacgatcc aacacaagat atctttatcg aattcaaaac agccacagaa
    16801 aaacgtatct ggatgatcca agctcatttg ggcaaagcgc cagagatcga cgcgtaaaaa
    16861 ctgttgttga acttgaccga aaaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16921 agaaacaaac gtcaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16981 cagctgattg aacaccgttt accaagaggt tgtaagcctg ccgttcagct ccgagtcaca
    17041 agaaacaatt ttaaaaaaca gcttcttatg ttttatggat attgtgactt gtgaccgagg
    17101 agttggcagg agaacaccgt ttattcaaaa taaggggttg tgacaagact tttgtcacag
    17161 cctctttttg tttttcattt tgacaggaaa cgaccctttt atataaaaga aagaaaagaa
    17221 gtaaagtttc atataaattt ttcaatcaag cagaaaaacg ttgacattga ctagattagc
    17281 gggtaatatg ttaaatggta ttgtttgccc cacaaagagc cccggaaact cgaagtgtat
    17341 gtcaaacatc agaacttaaa ttggaggaaa ataacgtgcg tacaacatat atggccaaac
    17401 caggcgaagt agaacgtaaa tggtatgtag tagacgcaac tgatgttcca ttgggacgtc
    17461 tttcaacagt tgttgcatct gtactacgtg gtaaaaataa accaactttc acacctcatt
    17521 tggatactgg cgatttcgtc atcgtcatca atgcagacaa agtgaaatta acaggtaaaa
    17581 aagcgactga caaaatttat taccgtcaca gcaactaccc aggaggattg aaatcaatca
    17641 ctgctggcga attgcgtgct aaaaattctc gtcgtttgat cgaaacttct gtaaaaggta
    17701 tgcttcctaa aaacacttta ggccgcaaac aatttacaaa attgaacgta tacggtggag
    17761 cagaacatcc acatgcagca caacaaccag aagttttaga catcacaaac ttaatctaat
    17821 aggagggaat ttcattggca caagttcaat atatcggcac aggccgtcgt aaaaacgcag
    17881 ttgctcgcgt acgtctagta ccaggaactg gcaaaattac tgtaaataaa aaagatgttg
    17941 aagaatacat tccacatgct gacttgcgtg aagtaatcaa tcaacctttc gctgttactg
    18001 aaacaaaagg cgcatatgac gtatttgtta acgtaaacgg cggaggatac gctggacaat
    18061 caggagctat ccgtcatggt atcgctcgtg cattgctaga agttgatcct gacttccgtt
    18121 tagctcttaa acgtgctggc ttacttactc gtgacgcacg tatggtcgaa cgtaaaaaac
    18181 caggtcttaa gaaagcacgt aaagcttcac aattctcaaa acgttaatat cctttatata
    18241 tcaacgtttc tggcactttt caattatttg gaaagtgctt cttttgtttt taagaacaca
    18301 tttaagaaca catatcaatc acacttattc aatcctctct gaaaagcttc aaaaacagcc
    18361 ttatcaacta tatctggtgt gttcacatat atctttgttg tagaaatatc tgagtgtgtt
    18421 aaagcatttg aaatttgatt catatctgct ccaccttgtc tagctatagt tgcataagta
    18481 tgacggagtt tgtgtggaga taaatgaact aagtcaggat gccttctttt aattgaattg
    18541 attcgatagt ttaggtaatc ggcatgaaca ggacaattta cttctccaga tggtttagta
    18601 taagtaaaga gatactgatc taacgtttgc ttaattttta atttcaacaa ttgatcagct
    18661 tgtaccgatt tccattcact tagcaatgtc attacaactt ctggtacttg aatgacggta
    18721 tcttttcgac cttttgtaaa cttttttctt tgatatttat ctaaagtatg tttaagacgg
    18781 accgtttgtt tttcaaaatc aatatatttc cattgcaaag cgtaagtctc actttttcta
    18841 tcaccaagat acagggtaat cataaaaagt gtgtaatcat gaaaagtaag taaatgatta
    18901 ttaaaatcat catttactgc ttctatccaa ttagtaagtt cttttgatga tagtgcttgc
    18961 ttaccttctt gtattctttt ttcttctaaa gcattttttt taggtgcagt gatactttga
    19021 ataattttag tagttcgatt aaattcgata taatttagta tttctgcaat atcgaacatg
    19081 gaacgaacat atccttttat gatttttata tttgcatatt ctttcgattt tttggttaat
    19141 tcatctgata ttatctcagt attgatattt agataattca ttgcgtattc accaaacata
    19201 ggtaaaatat gcaaccgaaa tatatctttt gtattactaa tggttatatc actaggaggc
    19261 ctgtttgatc taattgtttg acctaattca tatctaggaa gccatttaga ttcgtaaaat
    19321 tttttaaacg ttatttttcc ttttaattct aatgagtttg cattctcctc tctcaaaact
    19381 ttttctattt tcttcttcat atcattttcg gctttaatcg ccaattgcct atttggaaat
    19441 gttttttgat acttttctga tgcaattcct gttatttctt taaacctttt tggatatctc
    19501 agattagttt tatatttccc atttgaaagc tttgtaggca aaatcctcac tccttaaaaa
    19561 aagtaaggca gtatatttca ctaattttag tatatgcggc gagaagaaaa tttcaagtta
    19621 ttttaaatgt attcataaag tctttaatat cttctacgtc aaagcgaact acaccaccaa
    19681 ctcttattac tgggaaatta tatatacgaa tccacttatc tagagtatta ttatcgattc
    19741 ctagaaatgc acaggtttgt tttttattca ggtagactct ggattctata aaatcatctc
    19801 ctaaaatagt agttatgaaa tttttgaatt gctttgggta agcacatagg aatttcacct
    19861 ctccccagtt tacggcgagc cttacggaag tatcattatt tgctgctatt gtcatggcat
    19921 tttgttttcc cagaacaaaa agaaatctct aagtatttgt cgctaatcag gtcatggcgt
    19981 acttgagttt ctagctcgaa tagcagggat cgttattcaa aaattgtatt taattgtcaa
    20041 tgatcaaaaa tagttttttt cctattcact attaataacg ataaacatgt ataaaaagac
    20101 acactacgaa aaaagttaaa tggagtttac tgattttcat atttgtatat ggaacagaca
    20161 tagtcttgaa taatttttat aaatgttttg atgcttatca acttattcaa cccgctatcc
    20221 acttcaagta taatttttcc atacgccaat aaagtagttt taggagcatg ttctcatttg
    20281 agaacgctcc ttttttatat attttgaaag ctacttacta tacgcgtagt ttcaaatagt
    20341 tttgtcattc taatcttcag caacagaaaa aaatctaaac tataaaattt agggttgact
    20401 aaaatataat ttttgtatac catatgatcg tgataatcat tatcatttag aaggagaaag
    20461 ttatgttaga agtgaaaaag cttaatgttt gttataacga ttttttagca ttgaacgaga
    20521 tttctttaaa aatacaagaa ggtgctttaa caggtattat tggtccaaat ggtgctggaa
    20581 aatccacatt gcttaaagca atgttaaata ttatccctca tcagggagag gtctccatta
    20641 ataatgaaga tatcaataaa aaactggtga agattgcata tgtagagcaa aaagctgata
    20701 ttgattttac ttttccaata aaagtaaaag aatgtgtatc tatgggaaca tatgccggaa
    20761 tgaaagtttt tcaaagaatt aaaaatgcag aatggcaaag agtttcaaag gctttggaaa
    20821 aggtcgatat gggaaaatat tctaatcatc aaataggaga attatctgga ggacaatttc
    20881 aacgtgtact tttagcaaga tgtcttgctc aaaatgcgga tttcatattt ttagatgaac
    20941 catttgtagg gatagactta gttagtgaac gaataattat ggatacgtta aaaaaattga
    21001 aacatcaggg aaaaacagtg ctaattgtgc atcatgattt aagcaaggta aagaagtatt
    21061 ttgataatat tattcttttg aatcgaaatc taattgctca cggaagtgta gaatctgtat
    21121 ttaatgaaga aaatttaaaa aaagcctatg gagatactat atttattgga gagggggaat
    21181 aatatgcttc aaaattttat taatggtctg tatgactttc actttttaca gaatgctcta
    21241 attacatctg tagttatagg gattgtttct ggaacagtag gttgttttat tattttaaga
    21301 ggaatgtctc ttatgggtga tgctatttct catgctgttt tgccaggagt agcaatttct
    21361 tatattttag gtattaattt ttttattggt gcgattgtat ttggtctatt agcttctatc
    21421 attattactt ttataaaaag caatagtgta ataaaaggag atacagcaat tggtattact
    21481 ttcagttctt ttttagcact aggagttatc cttataggag ttgcaaatag ctcaacggat
    21541 ttatttcata ttttatttgg aaatatttta gctgtacaag atattgataa gtggattaca
    21601 attggcgttt cggttttagt gttggttata gtagttctat tttttaaaga attattaatc
    21661 acttcttttg atccattaat ggcaaaagca atagggatga atgtcagttt ttatcattat
    21721 ctattgatgg ttctattaac tttagtagca gtaacagcta tgcaaagtgt gggaactata
    21781 ttaattgttg caatgttaat tacaccagca gctacagcat atttatatgc aaatagcttg
    21841 aaaactatga tatttatttc tgcaacagtc ggagcaagtt catcattgct tggattgttt
    21901 attggttata gttttaacat agcagcagga tcaagtattg ttttaacagc ggcattctta
    21961 tttgtaatta gttttctaat ctcaccaaaa caaaaatttt taagaaagaa ggagagatcg
    22021 ttatgatgag aaaatggaaa gcagtactgg gaagtctggg gattttgatt gctcttttta
    22081 tattcggggc atgttcaata aatagtaaag acaaagataa agtggcttcg aacgaaaaat
    22141 taaaagtagt agttactaat tcgattttag cagatattac cgaaaatata gcaaaagata
    22201 aaattgattt acacagtatc gtacctattg ggaaagatcc ccacgaatat gaacctttgc
    22261 ctgaagatgt tcaaaaaact tcaaaagcag atttgatttt ttataacggt gttaacttgg
    22321 aaactggagg aaatgcttgg tttacaaaat tagttaaaaa tgcgaacaaa gaggaaaaca
    22381 aagactattt tgcagcaagt gatggcatag atgttattta cttagagggt cagagtgaga
    22441 aagggaagga agatcctcat gcttggttaa atttagaaaa cggtattatt tacgctaaaa
    22501 atattgaaaa acagttagcg gaaaaagatc ctgataataa aaaattctat aaagaaaatc
    22561 tagataagta tattgaaaaa ttggattctc tagacaaaga agctaaatct aaatttgctt
    22621 caattccgaa tgataaaaaa atgattgtta caagtgaagg atgctttaaa tatttctcga
    22681 aagcgtataa tgtgccttct gcttacattt gggaaatcaa cactgaagaa gaaggaacac
    22741 cagatcaaat aaaacactta gttgaaaaat tacgcacaac aaaagttccc tccttattcg
    22801 tagaaagcag tgtggatgat agaccaatga aaacagtatc aaaagatacc aatattccta
    22861 tctattcaac gatttttact gattcaattg cagaaaaagg acaagatggt gatagttact
    22921 atgcgatgat gaaatggaac ctggataaaa ttgctgaagg cctttcgaaa taatttggag
    22981 ggtaggtatg gtacaaatta aaacgactac tatactaatt tatttactag caattcagga
    23041 attatcaaaa agaaaaaaga aattaaaaaa taatgattta gcaaaagttc taagtgttag
    23101 tccagcatct gtaagtgaga tgctggctaa acttagatat gaggactatt tagatatagg
    23161 atttcaatta actgataaag ggaaaaaatt tattgacgat tacaaaaaag gttttaagta
    23221 aaagacttta tttttaggga ggataagaaa atggctattg gagtgattat gataggactt
    23281 atcctaatag ctggatataa tttttttaaa tctttctcgg attattttag aaaaggtgaa
    23341 tgatttagat gagtctatta attcttgtag gattgctttt tgtatcagtt attctgctct
    23401 ttctcattag tttagctcta aggaatgagt tcagtaataa acgttaacga ttgaaacttc
    23461 aagtcctgtg taagataata aagttaattc atcttattag gaggaagtac ccatagaaac
    23521 cagagattat attataactc accaaaagtt aatagaaagt ggactaagcc attttgaaaa
    23581 agaaggattt cagcaaacta agataaggca actgtgcctg accgcaggcg taactacggg
    23641 tgctttttat aaacatttta cttctaagga agcgttattt gaagaaatta ttcagccgca
    23701 tattaatgcg cttattaata tgt
//
LOCUS       DAYYOA010000032        23665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000032 DAYYOA010000000
VERSION     DAYYOA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.032"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACHBKA_001165"
     CDS             complement(1..120)
                     /locus_tag="ACHBKA_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9444869.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACHBKA_001166"
     CDS             299..1960
                     /locus_tag="ACHBKA_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9444870.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="ACHBKA_001167"
     CDS             complement(2030..2377)
                     /locus_tag="ACHBKA_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM9444871.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /gene="opp3b"
                     /locus_tag="ACHBKA_001168"
     CDS             2461..3402
                     /gene="opp3b"
                     /locus_tag="ACHBKA_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9444872.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="ACHBKA_001169"
     CDS             3403..4446
                     /locus_tag="ACHBKA_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444873.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="ACHBKA_001170"
     CDS             4452..5522
                     /locus_tag="ACHBKA_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444874.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="ACHBKA_001171"
     CDS             5512..6465
                     /locus_tag="ACHBKA_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444875.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="ACHBKA_001172"
     CDS             6477..7487
                     /locus_tag="ACHBKA_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9444876.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="ACHBKA_001173"
     CDS             complement(7772..8593)
                     /locus_tag="ACHBKA_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9444877.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="ACHBKA_001174"
     CDS             complement(8690..8968)
                     /locus_tag="ACHBKA_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444878.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="ACHBKA_001175"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="ACHBKA_001175"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM9444879.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="ACHBKA_001176"
     CDS             complement(11724..12311)
                     /locus_tag="ACHBKA_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444880.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="ACHBKA_001177"
     CDS             12486..13808
                     /locus_tag="ACHBKA_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9444881.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="ACHBKA_001178"
     CDS             13963..14739
                     /locus_tag="ACHBKA_001178"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9444882.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="ACHBKA_001179"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="ACHBKA_001179"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM9444883.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="ACHBKA_001180"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="ACHBKA_001180"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM9444884.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="ACHBKA_001181"
     CDS             16629..17258
                     /locus_tag="ACHBKA_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444885.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /locus_tag="ACHBKA_001182"
     CDS             complement(17382..18491)
                     /locus_tag="ACHBKA_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444886.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="ACHBKA_001183"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="ACHBKA_001183"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM9444887.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19309..19407
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19445..19981
                     /locus_tag="ACHBKA_001184"
     CDS             19445..19981
                     /locus_tag="ACHBKA_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9444888.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="ACHBKA_001185"
     CDS             complement(20021..21784)
                     /locus_tag="ACHBKA_001185"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444889.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="ACHBKA_001186"
     CDS             complement(21787..23502)
                     /locus_tag="ACHBKA_001186"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444890.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7631 a         4322 c         4802 g         6910 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt gttaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaag
//
LOCUS       DAYYOA010000033        23337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000033 DAYYOA010000000
VERSION     DAYYOA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.033"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACHBKA_001187"
     tRNA            2..74
                     /locus_tag="ACHBKA_001187"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACHBKA_001188"
     tRNA            78..150
                     /locus_tag="ACHBKA_001188"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACHBKA_001189"
     tRNA            155..225
                     /locus_tag="ACHBKA_001189"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACHBKA_001190"
     tRNA            238..311
                     /locus_tag="ACHBKA_001190"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACHBKA_001191"
     tRNA            323..394
                     /locus_tag="ACHBKA_001191"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACHBKA_001192"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACHBKA_001192"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM9444891.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACHBKA_001193"
     CDS             1983..3455
                     /locus_tag="ACHBKA_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9444892.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACHBKA_001194"
     CDS             3538..4326
                     /locus_tag="ACHBKA_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9444893.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACHBKA_001195"
     CDS             complement(4393..5061)
                     /locus_tag="ACHBKA_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM9444894.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACHBKA_001196"
     CDS             5176..6888
                     /locus_tag="ACHBKA_001196"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444895.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ACHBKA_001197"
     CDS             6891..8660
                     /locus_tag="ACHBKA_001197"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444896.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ACHBKA_001198"
     CDS             complement(8865..9431)
                     /locus_tag="ACHBKA_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9444897.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ACHBKA_001199"
     CDS             complement(9431..10387)
                     /locus_tag="ACHBKA_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM9444898.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ACHBKA_001200"
     CDS             complement(10387..11043)
                     /locus_tag="ACHBKA_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9444899.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="ACHBKA_001201"
     CDS             11413..13431
                     /locus_tag="ACHBKA_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9444900.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /gene="leuS"
                     /locus_tag="ACHBKA_001202"
     CDS             13858..16272
                     /gene="leuS"
                     /locus_tag="ACHBKA_001202"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM9444901.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="ACHBKA_001203"
     CDS             16620..18173
                     /locus_tag="ACHBKA_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM9444902.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="ACHBKA_001204"
     CDS             complement(18554..20338)
                     /locus_tag="ACHBKA_001204"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444903.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="ACHBKA_001205"
     CDS             complement(20353..22074)
                     /locus_tag="ACHBKA_001205"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444904.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="ACHBKA_001206"
     CDS             22174..22935
                     /locus_tag="ACHBKA_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9444905.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7376 a         4189 c         4854 g         6918 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatgg
//
LOCUS       DAYYOA010000034        23145 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000034 DAYYOA010000000
VERSION     DAYYOA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.034"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..573
                     /locus_tag="ACHBKA_001207"
     CDS             <1..573
                     /locus_tag="ACHBKA_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9444906.1"
                     /translation="LLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEP
                     KYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLI
                     PLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAA
                     AGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            585..1505
                     /locus_tag="ACHBKA_001208"
     CDS             585..1505
                     /locus_tag="ACHBKA_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9444907.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1546..3003
                     /locus_tag="ACHBKA_001209"
     CDS             1546..3003
                     /locus_tag="ACHBKA_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9444908.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3348..3962
                     /locus_tag="ACHBKA_001210"
     CDS             3348..3962
                     /locus_tag="ACHBKA_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9444909.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3955..5679
                     /locus_tag="ACHBKA_001211"
     CDS             3955..5679
                     /locus_tag="ACHBKA_001211"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9444910.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5676..7127
                     /locus_tag="ACHBKA_001212"
     CDS             5676..7127
                     /locus_tag="ACHBKA_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9444911.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7600..9753)
                     /locus_tag="ACHBKA_001213"
     CDS             complement(7600..9753)
                     /locus_tag="ACHBKA_001213"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM9444912.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9755..10801)
                     /locus_tag="ACHBKA_001214"
     CDS             complement(9755..10801)
                     /locus_tag="ACHBKA_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9444913.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            10951..12240
                     /locus_tag="ACHBKA_001215"
     CDS             10951..12240
                     /locus_tag="ACHBKA_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM9444914.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12253..14955
                     /locus_tag="ACHBKA_001216"
     CDS             12253..14955
                     /locus_tag="ACHBKA_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9444915.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            14940..15797
                     /locus_tag="ACHBKA_001217"
     CDS             14940..15797
                     /locus_tag="ACHBKA_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9444916.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15868..16725)
                     /locus_tag="ACHBKA_001218"
     CDS             complement(15868..16725)
                     /locus_tag="ACHBKA_001218"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9444917.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            16936..19107
                     /locus_tag="ACHBKA_001219"
     CDS             16936..19107
                     /locus_tag="ACHBKA_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9444918.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19175..19516
                     /locus_tag="ACHBKA_001220"
     CDS             19175..19516
                     /locus_tag="ACHBKA_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9444919.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19792..21546)
                     /locus_tag="ACHBKA_001221"
     CDS             complement(19792..21546)
                     /locus_tag="ACHBKA_001221"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444920.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21557..22393)
                     /locus_tag="ACHBKA_001222"
     CDS             complement(21557..22393)
                     /locus_tag="ACHBKA_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9444921.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22395..23078)
                     /locus_tag="ACHBKA_001223"
     CDS             complement(22395..23078)
                     /locus_tag="ACHBKA_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM9444922.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7610 a         4139 c         4765 g         6631 t
ORIGIN      
        1 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
       61 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
      121 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
      181 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
      241 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
      301 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
      361 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
      421 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
      481 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
      541 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
      601 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
      661 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
      721 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
      781 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
      841 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
      901 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
      961 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
     1021 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
     1081 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
     1141 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
     1201 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
     1261 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
     1321 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
     1381 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
     1441 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
     1501 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
     1561 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
     1621 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
     1681 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
     1741 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
     1801 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
     1861 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
     1921 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
     1981 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
     2041 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
     2101 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
     2161 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
     2221 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
     2281 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
     2341 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
     2401 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
     2461 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
     2521 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
     2581 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
     2641 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
     2701 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
     2761 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
     2821 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
     2881 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
     2941 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
     3001 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
     3061 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
     3121 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
     3181 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
     3241 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
     3301 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
     3361 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
     3421 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
     3481 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
     3541 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
     3601 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
     3661 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
     3721 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
     3781 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
     3841 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
     3901 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
     3961 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
     4021 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
     4081 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
     4141 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
     4201 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
     4261 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
     4321 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
     4381 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
     4441 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
     4501 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
     4561 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
     4621 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
     4681 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
     4741 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
     4801 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
     4861 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
     4921 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgagg
     4981 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
     5041 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
     5101 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
     5161 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
     5221 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
     5281 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
     5341 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
     5401 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
     5461 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
     5521 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
     5581 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
     5641 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
     5701 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
     5761 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
     5821 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
     5881 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
     5941 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
     6001 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
     6061 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
     6121 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
     6181 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
     6241 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
     6301 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
     6361 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
     6421 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
     6481 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
     6541 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
     6601 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
     6661 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
     6721 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
     6781 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
     6841 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
     6901 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
     6961 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
     7021 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
     7081 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
     7141 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
     7201 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
     7261 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
     7321 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
     7381 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
     7441 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
     7501 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
     7561 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
     7621 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
     7681 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc
     7741 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
     7801 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
     7861 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
     7921 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
     7981 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
     8041 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
     8101 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
     8161 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
     8221 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
     8281 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
     8341 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
     8401 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
     8461 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
     8521 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
     8581 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
     8641 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
     8701 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
     8761 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
     8821 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
     8881 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
     8941 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
     9001 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
     9061 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
     9121 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
     9181 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
     9241 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
     9301 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
     9361 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
     9421 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
     9481 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
     9541 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
     9601 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
     9661 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
     9721 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
     9781 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
     9841 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
     9901 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
     9961 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    10021 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    10081 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    10141 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    10201 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    10261 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    10321 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    10381 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    10441 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    10501 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    10561 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    10621 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    10681 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    10741 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    10801 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    10861 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    10921 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    10981 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    11041 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    11101 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    11161 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    11221 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    11281 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    11341 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    11401 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    11461 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    11521 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    11581 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    11641 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    11701 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    11761 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    11821 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    11881 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    11941 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    12001 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    12061 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    12121 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    12181 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    12241 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    12301 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    12361 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    12421 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    12481 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    12541 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    12601 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    12661 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    12721 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    12781 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    12841 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    12901 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    12961 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    13021 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    13081 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    13141 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    13201 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    13261 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    13321 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    13381 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    13441 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    13501 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    13561 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    13621 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    13681 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    13741 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    13801 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    13861 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    13921 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    13981 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    14041 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    14101 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    14161 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    14221 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    14281 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    14341 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    14401 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    14461 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    14521 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    14581 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    14641 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    14701 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    14761 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    14821 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    14881 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    14941 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    15001 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    15061 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    15121 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    15181 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    15241 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    15301 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    15361 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    15421 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    15481 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    15541 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    15601 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    15661 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    15721 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    15781 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    15841 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    15901 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    15961 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    16021 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    16081 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    16141 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    16201 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    16261 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    16321 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    16381 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    16441 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    16501 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    16561 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    16621 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    16681 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    16741 agtgcaattg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    16801 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    16861 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    16921 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    16981 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    17041 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    17101 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    17161 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    17221 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    17281 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    17341 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    17401 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    17461 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    17521 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    17581 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    17641 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    17701 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    17761 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    17821 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    17881 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    17941 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    18001 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    18061 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    18121 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    18181 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    18241 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    18301 cctgctgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    18361 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    18421 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    18481 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    18541 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    18601 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    18661 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    18721 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    18781 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    18841 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    18901 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    18961 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    19021 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    19081 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    19141 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    19201 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    19261 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    19321 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    19381 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    19441 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    19501 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    19561 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    19621 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    19681 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    19741 attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt
    19801 tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac
    19861 gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg
    19921 agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt
    19981 ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg
    20041 tatcaacgct cgtgcaatgg agatacattg cttttggcca ccagacagct tgttgccatg
    20101 ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc
    20161 agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt
    20221 ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg
    20281 cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt
    20341 cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc
    20401 aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt
    20461 gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg
    20521 gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga
    20581 agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga
    20641 attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc
    20701 ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc tgaaatatct gaaaactagc
    20761 ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat
    20821 ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc
    20881 aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct
    20941 gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag
    21001 aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa
    21061 agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt
    21121 ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt
    21181 ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc
    21241 tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg
    21301 accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc
    21361 tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag
    21421 atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag
    21481 atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc
    21541 ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag
    21601 gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga
    21661 tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct
    21721 ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt
    21781 ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg
    21841 cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta
    21901 actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat
    21961 tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag
    22021 taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg
    22081 cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa
    22141 cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt
    22201 ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa
    22261 gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata
    22321 ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat
    22381 ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag acgggattct
    22441 tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta
    22501 ttgtctttta gtccttctgg gtaattttta caagtatcag gcataagatg caggtagaat
    22561 ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct
    22621 aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt
    22681 atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag
    22741 accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag
    22801 ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt
    22861 acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg
    22921 ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa
    22981 aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct
    23041 gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg
    23101 tttataggca atgaagcaga acagcaagag aagaagacgg agcaa
//
LOCUS       DAYYOA010000035        22602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000035 DAYYOA010000000
VERSION     DAYYOA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.035"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..805)
                     /locus_tag="ACHBKA_001224"
     CDS             complement(<1..805)
                     /locus_tag="ACHBKA_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444923.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            943..2457
                     /locus_tag="ACHBKA_001225"
     CDS             943..2457
                     /locus_tag="ACHBKA_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM9444924.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2860..3843)
                     /locus_tag="ACHBKA_001226"
     CDS             complement(2860..3843)
                     /locus_tag="ACHBKA_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM9444925.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3848..4495)
                     /locus_tag="ACHBKA_001227"
     CDS             complement(3848..4495)
                     /locus_tag="ACHBKA_001227"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9444926.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4580..5212)
                     /locus_tag="ACHBKA_001228"
     CDS             complement(4580..5212)
                     /locus_tag="ACHBKA_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9444927.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5228..5779)
                     /gene="coaC"
                     /locus_tag="ACHBKA_001229"
     CDS             complement(5228..5779)
                     /gene="coaC"
                     /locus_tag="ACHBKA_001229"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM9444928.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5776..6513)
                     /locus_tag="ACHBKA_001230"
     CDS             complement(5776..6513)
                     /locus_tag="ACHBKA_001230"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM9444929.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6510..7499)
                     /locus_tag="ACHBKA_001231"
     CDS             complement(6510..7499)
                     /locus_tag="ACHBKA_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9444930.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7757..8299
                     /locus_tag="ACHBKA_001232"
     CDS             7757..8299
                     /locus_tag="ACHBKA_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM9444931.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8316..9401
                     /locus_tag="ACHBKA_001233"
     CDS             8316..9401
                     /locus_tag="ACHBKA_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9444932.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9413..10219
                     /locus_tag="ACHBKA_001234"
     CDS             9413..10219
                     /locus_tag="ACHBKA_001234"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444933.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10220..11047
                     /locus_tag="ACHBKA_001235"
     CDS             10220..11047
                     /locus_tag="ACHBKA_001235"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9444934.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11044..12117
                     /locus_tag="ACHBKA_001236"
     CDS             11044..12117
                     /locus_tag="ACHBKA_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9444935.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            12545..15241
                     /locus_tag="ACHBKA_001237"
     CDS             12545..15241
                     /locus_tag="ACHBKA_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9444936.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            15306..15806
                     /locus_tag="ACHBKA_001238"
     CDS             15306..15806
                     /locus_tag="ACHBKA_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9444937.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            15818..16312
                     /locus_tag="ACHBKA_001239"
     CDS             15818..16312
                     /locus_tag="ACHBKA_001239"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9444938.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            16333..17142
                     /locus_tag="ACHBKA_001240"
     CDS             16333..17142
                     /locus_tag="ACHBKA_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9444939.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            17139..17960
                     /locus_tag="ACHBKA_001241"
     CDS             17139..17960
                     /locus_tag="ACHBKA_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9444940.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            18011..20110
                     /locus_tag="ACHBKA_001242"
     CDS             18011..20110
                     /locus_tag="ACHBKA_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM9444941.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            20107..21225
                     /locus_tag="ACHBKA_001243"
     CDS             20107..21225
                     /locus_tag="ACHBKA_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9444942.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            21264..22142
                     /locus_tag="ACHBKA_001244"
     CDS             21264..22142
                     /locus_tag="ACHBKA_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9444943.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            22288..22485
                     /locus_tag="ACHBKA_001245"
     CDS             22288..22485
                     /locus_tag="ACHBKA_001245"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9444944.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         7022 a         3909 c         4842 g         6829 t
ORIGIN      
        1 atcgtttgat ttttttagga ataaaattct tgatgaacgt aaatttttgg taatcgtttc
       61 gactatagag gtaaccaaat acactgaaaa caactgctcc aataaaatta acaaataaat
      121 ccttcatagt atccatgatt cccacgtcta agtaaccatt ttggatgatt gttttatctg
      181 ttttaccatc tgcattttga ctttcaatga ttgtccgagt gatatctgga acatgataga
      241 cagtgtttcc gtctttactt aactccacac tattgatttg ctggatgatc gtatcttttt
      301 gcatatcgaa ttgcagtaat ttatctgctg aaaattccac aaactcccac ataacaccaa
      361 ctgtcatcga aaaacagaag gaaacaactg ctagtaataa aggagtcaag ttcatggttt
      421 ttgtattttt attgagcaaa taaacaaggg aaaagccgac acccccagct aaaaatccgc
      481 tgaatgcatg tagaaaagta tcccacattg aaaaatagcc ataaaaatca cttaactctc
      541 ctaaaactgt tgaactaaaa ataaaagata acaaaatgat ttctagcata tttggaatct
      601 gtatctttaa tatcttttcc agaaaataag ggatttcaaa taaaattaat gtcagtaata
      661 aaagcattgt atttccccag tgttggttcc agacctgatc cactagcata ataacgacga
      721 tgatacgtaa taaaatatag attccgtttg tgattctacc tgcagttttt gtactgttcg
      781 taaaaaaggt tttcaaatga ttcataaaat aactccttca tgtatatcct taattatact
      841 aagaagcgtg tgtttaccaa agctatatga cttaaagaaa aacagagttg atattgtgaa
      901 aaaaatcact taatatagta taaggagaaa agggggatac aaatgaaaaa attagttgct
      961 ggtttattaa cagccgttgc acttcttgct atgagcgcat gcggcgcaga tcagaaaaat
     1021 acagcgcaga caagttctag tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa
     1081 caagtatata cagatccaaa agaaatgaaa gaatcttatg atgtcgtgat tattggagct
     1141 ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt
     1201 ttggaaaaga tgcctgttgc cggaggaaac acatctaaat catcaagcgg tatgaatgct
     1261 tcagaaacaa aatttcaaaa agaagaagga atcaaagact cgaatgatgc cttctatgaa
     1321 gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa tgcttcgata ctttgtcgat
     1381 cactctgctg atgcggtcga ttggctagat caaaacggta tcaaactgga caacttaacg
     1441 atcactggag ggatgagtga aaagaggaca catcgtccgt ctgatggctc ggctattggt
     1501 ggatacttag tggaggggtt attgaataac gtccatgagc gggaaatccc tatttttgtc
     1561 aacgcagatg tgacagacat taagaaaaat ggggaaacgg tcaatgaagt gactgtacat
     1621 gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt
     1681 tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg atttagctga ttatattacg
     1741 acaaatcaag aaggatcaac tggagatggt atcaccatga ttgaaaaatt aggtggacaa
     1801 accgttgata tggataaaat acaaattcat ccaactgtgc agcaggatga gggggtcttg
     1861 attggtgagg ttgttcgcgg agaaggagca atcttggtcg atcaagaggg gaaaagattt
     1921 gtcaatgaaa tggatacaag ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa
     1981 tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat
     2041 gaccaaaaag gatatgtcac aactggaaag acaatggaag aattagcaga aaaaatcgat
     2101 ttaccaaaag aaacactagc aaaaacagtt gatggatgga atacggatgt tgcggagaaa
     2161 aaagatgctc aattcaaccg tgccacagcc atggaacatg acttatcgtc tccgaattat
     2221 tatgcgatca aaatcgcacc aggaatccat tacacgatgg gcggtgtgaa aatcaatacg
     2281 aacactgaag tattagataa agaaaaccag ccaattaggg ggttatatgc tgctggtgaa
     2341 gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa attcagttgc ggagatcatt
     2401 atttttgggc gacaagcggg catacaagca gctcgatttg cttcggaaaa tgaatgaaat
     2461 agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga gctaataccg agggtcagct
     2521 gattgaacac tgtttagtga agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     2581 cagccaagta aaaacagctt cttatgtttt taacttggtt ggacttatta ccgcagatgc
     2641 aagcttttga acaccgttta ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt
     2701 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag aaattccttc ggaaataagc
     2761 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
     2821 aacgcttctg tcccagcctc ttttttgatc agcatgattt tattcagtta tcttcaaaat
     2881 gatttttccg atgtttttgt ttgcttccat atattcatgc gcttgtttga ctgcgctgaa
     2941 ttggaatgta cggtcaatga ttggccgaat cttgccattt tctagaaaag gcccagcttt
     3001 ttgcataaaa gtttgtgcga gctctgcttt atactgatca cttctaggtg tcaataatgt
     3061 accaagcagt tggacacgtt tcccaatcaa tacaccaagg tctatctcag gcacgatggt
     3121 cccacctagc ataccaatca agacccaacg tccatcaacg gctatagaag ctaaattctt
     3181 ttcccagtaa gaagcgccga tgaaatccaa aataacatcg actccccgac cttcagtgat
     3241 ttttcgtacc tcttcttgaa agtcttgttt tttatagttg atcaattcat ctgcaccaag
     3301 ttctttacaa aaggcgagtt tttcttctga tcctgcagta acgatgattt tcgcgtgaga
     3361 aagctgtcta gctaattgga tcgctgctgt tcccacacca cttgctcctg catggatcaa
     3421 gacagtttcg ccttcttgta attttccaag ccagtaaagt gtttgataag cagtcaaaaa
     3481 aacttctgag attccagcag cagaaacata gtctagcgta tcagggagaa ggattgcacg
     3541 atcggcaggc atcacagcat attcggcata accacctaga ttgactaatc cgtatacttt
     3601 tgtcccagga gtaaaagtgg agatatcact tttattctca actacctctc cagctacttc
     3661 gactcccaaa atagggtagg gaggtttcaa agagactgat tctcttttca taatatctgt
     3721 tcgattgaca gctgctgtat ggactttgat cagtaattcg ccttgttttg gtttaggaac
     3781 aggcacttca atttgttcta atacatctac agaaccagga tgcttgattg aaatggcacg
     3841 catggatcta ttcctccttt gcccattctt ggaatgctgt attcaactct ttgctacctt
     3901 cagaaagtac tagagtcgta cgagaaatat ccgtttcttc cactagagcg gcaagtacag
     3961 cagcaactgt agagcgagtg atcgtttttg atgccttctc attaggctta gttaattgaa
     4021 tggaagtaac ttcttcatcg ttagtcaatg tgactggttg aagaatgaca aaatctaagt
     4081 tagttcgttt cagccattca tctgcataat gtttagtaat ataataatct gtcatggact
     4141 caggccattt tgctggttca tctgcataaa cagcactgac catcagatag cgagagacat
     4201 gcgcattttc agctgcgatc actgttttga ttgcgccatc caagtctact tgcagaagat
     4261 tctttccttg agagccggca gcaaaaatca atacatcgat gcctttgaat gcttcggtca
     4321 ttttttccac ggaccaagtt aaatcaaaag agacatacga aacattctct ttttttacta
     4381 atgtttggga agcaacatct ctcacccctg caaaaatttc atgggaactt ggagctaaat
     4441 cttttatcag gtgtcggcca atttgtccgt ttgcacctac aacaaaaatt ttcatttgga
     4501 tcatcctttc gacgtcaaat ctttccattc aatagtaaca ccaaaagaga aaaaaatcag
     4561 agatcggcta cgatgactat cagttagtaa gagaagggtg cttgaagtat cttaacaaga
     4621 cattgcatac tgctgcagtc gcaagacctg cgacgattcc ttcaggtata ccacttgtaa
     4681 aaataaccgc tagaatagct gaataaacag catttaaatt tgttccgatg actttggcat
     4741 aatcttgctg gaataaaaag tagatcatat tcatcactaa aatcgtattg gtcaatgaac
     4801 cggaaagtcc agctaagaat aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca
     4861 gacctttata aacgaaataa ggaaccaccc caatcaagat gcgaggaata aacgcgatca
     4921 gcaaggcttt ccagcttcca tgttctgttc cgatgactgg gataaacgga gaaaagacaa
     4981 aagacaaagg tgtttggata actgtgctac ggatcatgct gatcagtcca aatgtaccgc
     5041 caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca
     5101 tggttgttgc attgattgga ccgataggga taaaacctaa aggcgtaaca gctaataaaa
     5161 tcaaaatagc taaaaacatc gcagttaatg taaagttttt tgtatttttc ataagtactc
     5221 cttttttcta agcatttctt tcattaagag tcagcatgac ctgttcgatg atcgtctgaa
     5281 tatcagctaa ggcacctttt ccaaaatctc cacaagctaa aagagattct cttggttcaa
     5341 tttcctgata gccgacagat ctcaatgttt ccagattacg ctgagtgatc gggttttgat
     5401 acatattcgt gttcatggca ggggcaatca attttggtgt tcccactgga agcgccattg
     5461 cgactgttga aagaagatcg tctgcaatgc cgtttgctag cttgccgata atgtttgctg
     5521 tagcaggcgc gatgagaaac aagtcagctt gttttgctag ttcgatatga ttgattacct
     5581 gcggatttga ttcttgcatg acgtctgtat ggactgcgcg cttcgacaga gattgcaagg
     5641 ttaatggcgt gataaaatgt gtgctgcttt tagacataat cacatctacc tgggcaccta
     5701 atttgacgaa ttgacttgta agatcagcgc ttttgtaagc agaaatactt ccagtaacgc
     5761 ctagtaagat ctttttcatt ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc
     5821 aatctcagat ttactttgag cttcttccac tgttccatct ttagataaga gatagccgtg
     5881 gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt
     5941 tatcagacta ttttttgcga ctgccagaag agattccttt ggtacatcta ctaatagttt
     6001 gaaaccaatg atttttgctt ttggctgcca ttcacgtaga taagaaatga tttttggtgt
     6061 tttcttcaaa acgagcacaa gatgctcggt gttagaagag atctttgaaa cagtggtatc
     6121 tgtctgttca ttttccgcaa accaattgtc cagttcttct tgcgttgaag aagtgggtag
     6181 gttttttgcc agctgttctt ctgaaaaact aaaagcagga gtaaaatcac tgactgccat
     6241 actgtggata atcgcatcgt agtgttcttt tttcgttagt tcttctaatt gtaaaaataa
     6301 gtccagtgca gattcgatcg tataaatcgt tatattggag tgtcttttcg gtttcagtgc
     6361 ttgtttcgta gtcacgtaat cgattgtggt atccggattg gcagctaaat aatcagctaa
     6421 tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca cgtacttgat cgattttttc
     6481 agtcgttcct ccagctgtga ttagaatctt catttatggt cacctacatt tactagtgtt
     6541 cttccttggt gtgttccagc aagtagttta gatgctgttt caggcaattg atccagagta
     6601 atctcttgga cgaccagttc atctgcaatc tgcaaatccg ccatctgttg ccataaagaa
     6661 agacgttgat ctattggtac attgacagaa tcaattccaa tcaactggat gtttcgcaga
     6721 ataaaaggca agacagtcgc gtttagtttg attcccccag cattgccaca taaagctact
     6781 gcaccgttat aagagatcaa tggaagaaga ctgcttagct gctcgccacc tactgtgtcg
     6841 atgacatagt cgatttgctg ttttcctaaa gcttttgttg tctctggcag aaattcatca
     6901 ggtcgtacga cttgcaaagc acctagactt tttagccatg tgacagactc ttcttttctg
     6961 gaaaaggcaa tgattgactg gaatcctagt ttatgtaaca gggctatcgc tgtactgcct
     7021 acaccgccag aagcaccagt aacgacaact tttttatcgg aggtcatacc ttgacggatt
     7081 aaagcgttca cacacaacat agcagtaaac cctgctgtac caaggatcat cgcttgacgc
     7141 agcgtcatat tttttggtag aggaactaac cagtctcctg gtactttttg atattggcta
     7201 taaccgcctg gatgggtgac gccaagccca aaaccagtag caagtacaag atcttctttt
     7261 gaaaaacgtg gattgttact ttggacgaca gttccggata aatcaatccc tggagtcatc
     7321 ggatatgttc gtatcacctg accagactcg ctcattgcca atgcatcttt ataattgaca
     7381 tcagaatagg caatttttac caagacttca ttttcagcca actcagtaaa tggcagatca
     7441 ctgattgctg tatggaaaac aggttccttt attacctgaa aagatcgaaa agcgttcatt
     7501 cgtgagacac tccttttaaa attttctttt atcataccat aaagcctact cttttattct
     7561 tgttcttccg taaatttaga caaaaattag gtgaaaaata aagaatcaaa caactagaat
     7621 ttattccaaa agataaagaa atattcagca aaaaaaattt tacaaagggt tgcctttttc
     7681 aataaatttc gtatactaat acgagtggta gtaaacagga agtttactat tagaaaactt
     7741 cgattcggag gaaattatgg aaattggtga gaagttaaga aatttgcgca tccaaaagaa
     7801 tttgacacaa gaagaactag gcgaacgcac agatttgtca aaagggtata tttcccagtt
     7861 ggaacgagat ttaagttcac catcaatgga aacattcttt tctattcttg aagtcttagg
     7921 tgtgactcca gaagaatttt ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa
     7981 agaagatcat acggtttatt tcgatgaaga aaacgggtat gaattggaat ggctcgtggc
     8041 tgattcgaat gaaaaagaaa tggagcctgt cttgctgact tttgaaggtg caggagaata
     8101 taagacgttt gaaccttcat tatctgaaac gtttattttt gtattagaag gggaattaga
     8161 gttaagctta ggagagacgg tctatgtggc aaaagcagga cagtcgatct attatcaagc
     8221 gacaaaacaa catcaattaa ggaatcatct caaaaaacga acaaaagtat tgattgttgc
     8281 aacgcaatca tatttgtaat atagggggta aaaacgtggg gaaaccaatc atttcatttg
     8341 atcaagtagt gaaacgctat gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg
     8401 aacaaggaaa attttataca ctgcttggtc cttccggctg tggtaaaaca acgatcctac
     8461 gtattatagc tggattcacc gaagctacag aaggggacat ctattttgaa gggaaacggt
     8521 tgaatgattt gccagctaat aagcgacaag taaatacagt cttccaagat tatgcacttt
     8581 ttcctcatat gaatgttttt gataacgttg cttttggatt gaagatcaag aaaatgtcca
     8641 aatcagatat cgaaaagaag gtaaaagatg ctttgcgtat ggtccagctt cctggatacg
     8701 aaaaacggga gattagtgaa atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag
     8761 caattgtaaa tgaaccgaaa gtattgctat tagatgaacc gttatcggcg cttgatttga
     8821 aactacgtac agatatgcag tatgaactgc gggatctgca acaacgatta ggaatcacgt
     8881 ttatttttgt tactcatgac caagaagaag cgttggcaat gagtgatgag atttttgtca
     8941 tgaataaagg gaaaatcgtc caaagtggta cacccgtaga tatttatgat gagccaatca
     9001 atcatttcgt tgctgatttt gttggggaga gcaatatcgt ggacggtgta atgatcgaag
     9061 ataatctagt tgagtttgtt ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa
     9121 atgaaccagt tgaagttgtt ttacgcccag aggatttgac gatcacgaca ccagataaag
     9181 gaaaactagt cgtaacggtc gatactcagc tgtttcgtgg tgtccattat gaaatcatct
     9241 gttatgatga acagcaaaat gaatggatgg tccattcaac gaaaaaagct aaagaaggaa
     9301 gcaaagtagg attggccttt gagccagaag atatccatgt catgcgtttc aacgagtctg
     9361 aagaagaatt cgatgcacgt cttgacagct acgaagaata ggggggacaa gaatgaaaac
     9421 aatgcgcagg atctattccg tcccttatat tttgtggctc ggtttattcg tgatcgcgcc
     9481 tgtattaatg atcatttatc aatctttttt tgatatgaat gggcaattca cgctgaataa
     9541 ttatctatct tattttacgt cggggacatc gctttctatg acgatcaatt ctgtatggta
     9601 tgcatttttg atcacgtttt tcacgctgat catcagttac ccgactgctt attttttaac
     9661 aaaactgaag cacaagcaat tatggctgat gctgattatt ttgcctacat gggtgaatct
     9721 gctgcttaaa gcttacgcat ttattgggat tttcagtgtg cacggcagcg tgaatcattt
     9781 ctttgatttt atcgggatcg ggccgaaaca gatattattc acagacttca gttttctgtt
     9841 cgtggcaacg tatatcgaga ttccatttat gattatgcca attttcaatg cattggaaga
     9901 gatgaatcct tctttgatca gtgctagtag agatttaggc gcgaatagct ttgagacctt
     9961 ccgacgagtg atttttccgt tattattaaa tggtgtgaaa agtggtgtcc aagcagtatt
    10021 tattccttct ctttctttat tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac
    10081 tttaggaacg gctatcgaac agcatttctt ggttacacaa aactggggga tgggttctac
    10141 aatcggtgtg attttaatca ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa
    10201 aaaaggaggt cgctcataaa tgaaaaaatt taaatggtcg tatctttatt tagggatcgt
    10261 ctttttattc ttatatgcac cgatcttcta tctgattttt tattccttca atgaaggagg
    10321 aacgatgaac agctttacag gatttacttg ggaaaattat gaagctgttt ttgaagatac
    10381 acgtttgatc attatcgtat tgaatacgtt catgctggct ttcttatctg cattgatcgc
    10441 cacgatcatc gggacatttg gggctatggg aatctattac acgaaaaaac gacgtgcacg
    10501 gacagcgttg ttgagcatga ataacatctt attagtctct cctgatgtta ttatcggtgc
    10561 tagtttcttg attttcttta cactactagg attcggatta ggttttcaga gtgtattgct
    10621 ttcccatatt gctttcagca ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga
    10681 aatgaacgat tcaatggtcg atgcggctag agaccttggt gctaataatt tccaagtagt
    10741 caaaaatatc attttgcctt atctttctcc aggaatcata gctggatatt ttatggcatt
    10801 tacctattct cttgatgatt ttgcggttac atttttcgtc acaggaaatg gctttacgac
    10861 gctctcagta gagatctatt ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct
    10921 aagtgcgtta gttttcttgt tctccatgat attggtcatt ggatactact ttattagtaa
    10981 agaaaaccgt ccaaaacggc tgaagaaaaa cagaagagta cgcgaggggg tggcacggct
    11041 gcgatgaaaa aattacaatc tctgttttta ggtattctca tcattattgc ggtgctgctg
    11101 cttggtgttc gtcaacttga aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc
    11161 tataactggg gagattatat cgatcctagt ttgatcacga aatttgaaaa agaatatgga
    11221 tacaaagtga attatgaaac atttgattct aatgaagcga tgttcacaaa aatccagcaa
    11281 ggtgggacaa actacgacat cgctatacca agtgaatata tgatccaaaa aatgatcaaa
    11341 gaaaaactag tacttccatt agatcattcg aagatcaaag gattaaataa tattgatcca
    11401 cgatttttag acttggattt tgatccagat aatcagtatt cgattccata tttttgggga
    11461 actttaggga tcgtatataa cgataaattc tttgatgcaa atcaaattaa gcattgggat
    11521 gatttgtgga agccggaatt aaaagacagt ttgatgctga ttgacggtgc acgagaagtt
    11581 atggggcttt ctttgaatag tttaggctat tctttgaata gtaagaatat ggcacagttg
    11641 acagaagcag caaataaatt gaatcattta acaccaaatg tcaaagcaat cgtggcagat
    11701 gagatcaaaa tgtatatgat caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa
    11761 gctgccgata tgatgtggga aaatgaacat ttacattatg tgatcccacc agaaggatcc
    11821 aatctttggt ttgataatat cgttatgcca aaaaccgtga aaaataaaga aggtgcctat
    11881 gatttcatca actttatgct agaaccggaa aatgcggcac aaaatgcaga gtatattggt
    11941 tattctacac caaatgaaga agcaaaagcg ttattgccaa aagacatttc gtcggacgaa
    12001 caattctatc catctgatga cacgatctcc catcttgaag tctacaaaga tctaggatcg
    12061 aaatacctag gcatctataa tgacctattc ttagaattca agatgtatag gagataatgg
    12121 caaaactcag taagaatggg gacttatcaa cgttctaaag agctaaaaat cgtccttttt
    12181 ctatctgagt tttccaatct tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat
    12241 tttatttttt ctaccacgtt tttctaccag agaaaagtgg acaaaaccat tgtaaaatag
    12301 ggcccgagac gggctagtaa atctaagatt gaaaaagtta cagaaggata aagtaattgg
    12361 aaactatgca aatatactta acccttctgt aatctattaa taaaactaat atatgaatcc
    12421 agttcaatat ttgtagctat gaaaaaacat aaaaatcata ttagataaaa actaaattgg
    12481 tagataaatt attttgtcag atatctgagg caaatattcg actatttgtg tttcaaagta
    12541 aaaattggaa tacaaatata gaatagacat attgcagccg atagtttatt atgcttttag
    12601 acacattaaa ctggaggaaa aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct
    12661 gatacgacta acagggaata tgtcttctgt cactgccgag aaaatttcga agaatctagg
    12721 tatctctcgt cagaatgcca gccattattt gacgagatta gtggaagata aaaaggtcga
    12781 gaaattaccg ggaaagcctg ttttgtggaa gcctttagat gaatatgcgg ttgtagataa
    12841 tacggagcaa ataaacgaag cctttcattc tgtagttggt catgacggaa gtctgagaga
    12901 ggtcattcaa aaatgtatag ccgctgtcaa atatccgcct aacggactca gtgtattaat
    12961 aaatggagcc acgggtgttg gaaaaagttt tttagccaca aaaatttttg aatacgctat
    13021 ccatgagcaa attattgaaa aagatgcacc atttgctatc ttaaactgtg ccgattatgc
    13081 ggacaatccc gaactgcttt cggcaacact gtttggatac aagaaggggg cctttacggg
    13141 ggctgaaaag gataccgatg gtttattggc aacggccaac aacggctatc tttttctcga
    13201 tgaggtccat cgcctatcga aagaaaatca agaaaagctc tttctattta ttgatactgg
    13261 aaactatcgt ccagtcggtg aaaatgtaaa ttggcattct gctaaggttc gttttatttt
    13321 cgccacgacg gaaaaaggtg aaaattattt attagacacc tttgatcggc gtattcaaat
    13381 tagtgttatg ttgccaactt ttgaagatcg ccccattcga gaacgattag agctgattca
    13441 attatttttt caaaatgaag cggatgtttt acaaaaagat attattgttt caagagaagc
    13501 cttgtcttta atgttatctc atccctttag tgggaatatt ggtaagctga agaatatcat
    13561 taaaatcagt tgtgcggatg tgtatagtcg aacacaagaa gaaccattaa cgattgggaa
    13621 aaatgagatc ttgattcagt taaatatgct ggaatccgaa gtagaaagct tgccgattgc
    13681 tagtttattg ataaagaaaa atcaaaagat aaaaacggat cgaatcaaac cgtttgatta
    13741 tgattcgatt ttggaaaaaa ttactcagct gataaatgaa ggtaatttca atgaaaattt
    13801 ttcttcgata aagaatgaag tacagcattt ggtgcatcac attgataagg attctttttt
    13861 atcaatgagt agaaaattga tgattcagat gtttagaaag gtctgggtat ccgttttagg
    13921 aaaaacgtac ggattgacga cgagtggacc tgttgcagaa attattagca gatcctatgt
    13981 catttcccca agctataaga cagacttaac cgggataaat cgtacattag ctgcaaatct
    14041 acctcggacg acttacttat gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa
    14101 ggacgagaac tttctaagac tgcttattgt cgtgacttta tctgattatg tagatgaatc
    14161 catcgagctg aagggcctgt tgcttgctca cggagatagt acggctagca gcattcaagc
    14221 agtagtgaat caattatgcg gcaattatgt ttttgaagcg ttggatatgc cagtagagag
    14281 taatgtgtca gagattgtcg aatcgacaag gaatttcgtg aaacggcaag tgaatacaga
    14341 tagtctagtg ctgatcgtag atatgggctc tttaaatcaa atttacacac agatcaagaa
    14401 cgatttaaaa ggggagttac ttcttctgaa taatttgacg acttccattg cacttgatat
    14461 tggcttgaag atgacgatga atgcgccatt taaacagatt gcggaggatg cagcagacaa
    14521 gtattcaatc aatgttcaat actttgaagg cttttctcaa agtagcaaca ttatcatttc
    14581 ctgtatgtcg ggtttgggga tttcggacaa attgaaagat gtttttcgtc atcatttatc
    14641 ggatgaatcc atcgaagttt tcacgaagga ctacagagaa ctacgtgaat taatcgacca
    14701 aaatgatgaa acttattttg acaagacaaa attggtcatc acgacttcgg atcttccacg
    14761 ttccttttca attcctaata ttaatgttta tgacatctta gacggtgatg gagcagccaa
    14821 tttggagcat attttgagtc ctgaaatgaa gccggcagaa tttaaccgct tgattcagga
    14881 gctagtaaaa ttcttttcgt tggaaggaat tgcggaccgt ttgaactttc tgaacccaac
    14941 gatcgtgatg agtgaagtgg aaacagtcat ttctaaatat gaaaattact atcatttcac
    15001 tttaaatggc cgtgtaaaac taaatctcta tatgcatacg gcattgatga tggaacgttt
    15061 attcttggct cggacagaaa aatctgaaga accgaattat gatttgaaac cagatgaaga
    15121 tgaattttat tatgtttcta gaagtatttt tcaatcaatg gagatgaaat ataattttaa
    15181 agtcaatgct tatgagcttt cgctgctcta tgaattatta ggaccgtata ttcaaaaata
    15241 aagtgtctaa aaaagtgtct aatctgtttt ttttaggtta gacacttttt ttaatgtgaa
    15301 ttaacgtgat taaaccaatg tttttaaaag ttggcacagg aattgcttat ataaaagtga
    15361 acagaaagaa aggaattaac catatgaaga aaattctaat agcgactcac ggtcacttag
    15421 cttcaggctt tcttagcagt attcaattac tgacggggaa ggttcaagag attacggtga
    15481 ttaatgctta tgtggatgag tgtgattttg aacaggagtt gaatgtattt gtaaaccagg
    15541 tgaacgagca ggatcagatt tttgttttta ccgatttatt tggcggaagt gtcaatcaga
    15601 agattaccaa aacctttttg gagaaagaga ttgctgtaac tattttgtct ggctttaatc
    15661 tgccaattct gctcgaaata gtattggcaa ccaaggaatt aacaaaggag gatattcagg
    15721 aactggtaaa taaatgccaa caggaattaa agatgaccat tcttgatgag gtacatgtag
    15781 taaatgaaga ggaagatttt ttttaggagg aagaaaaatg attaaagcag tgcgaattga
    15841 tgaacgatta attcatggac aagtagcgat gacttggaca aagagtctaa atttaaccgg
    15901 gttagtagta gcaagcgatg aggcggctac caacgatatt caaaagatga cattgaagat
    15961 ggctgcacca tcgaatgtca aagtcattat taaaacagtt gatgacgcga ttcatttgtt
    16021 aaaagatccg cgggcgaagg acatgcgctt gatggtttta gtccctaatg tcaaagatgc
    16081 ggtaaaagtt gcgaaagctt tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg
    16141 gaaaatgaca ccgccatcgg aagataagaa aaccttatca aaggaagtga tgttaacgac
    16201 gtcagaaatt caagacttaa aagaattagt tgaactttat ccagataccg tttttcagcc
    16261 tacaccggcg atggaaaaac gttcagctat ctctattttg agtgattttt agaaaaagga
    16321 aggagaaaaa ttatgtatga tgcatttgta gtcgctttgg ccgtttttat tggaattgct
    16381 ggacatgaaa tgttcgggat gtccatgctg agtcgtccga ttgttgttgc tccgttagca
    16441 ggagcgttga tgggggattt gcagacgggg ttgattgtcg gggcgtcact agaagccatc
    16501 tttatgggtg tcgtcaatat cggaatctct agtacggcgg agccttcttt ggctgctggg
    16561 ctggctacag cgttcgcaat caaaatggga ggaaacgtgg atgcgattat tcccattgtc
    16621 tttccactag cagtattagg attacagtta atgaatatga ttttttcttt tgtatgtgga
    16681 ccgatggctg ctaaattttt caaatttgca aaatctggtg atcaaaaagg aatcatcaat
    16741 ctgcacttct ttatgtggtt tgtccactat gcgctttatg ctcttattcc attctttgcc
    16801 gttttatttg gagcagatgt tgtcaaaagc gtcttggata atattccaga ggtcatcatg
    16861 aacggattaa cagtcgctgg aaatctgctg ccggcagttg ggatggcgat gctgttacgg
    16921 atgctttgga gtaaaaatat tgctgtgtgg tttttctttg gggccgttgt tatggcgtat
    16981 ctcgagttac cattgattgc aattgccgca gttggaacga ttattgcaat tgttatagcc
    17041 caaagagatc tacaactaaa aaatatatct gttcatgcag ttcaaagcga tgctgctgta
    17101 aatgcaacgc ttgaccaaga agaggaggat ttctttcaat gaaattagct gatggaattt
    17161 ctaaagatga gaaaaaattg gttcggaaga tgttttggcg ctcgatgact atgtatgctt
    17221 cggtgaatcc ggtgacgatg ggcggcggtg gtttcgccta ttctatgatg cccttcatta
    17281 atcatttcta caaaagtgaa gaagatcgag ctgcggcgct ggaacgtcat acacagtatt
    17341 tttcgacgac cattccttgc gcgtcctttg tcatggggat tgcggcatcg atggagaagg
    17401 aaaattctga aaaagctggt tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga
    17461 tgggaccatt ggctggaatt ggcgattcat tgttctgggg agtttggcgt gtcgttaccg
    17521 cgggactagc gatcaactta gccaatgccg gaaatatttt ggcaccaatt atctttttag
    17581 ttatgttcaa tatacctaat tacttgatgc gatactatgg cggcgtttta ggctattcat
    17641 taggctctaa atatattgaa aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag
    17701 ctgctagtat cttgggtctg atcatggtag gtgcaatgac gatcagtatg gtcaaattca
    17761 ataccgtttt gagcttttca atgaagggcg aagaactaat gaatgtccaa gaaatcctcg
    17821 atagtatttt taaaggaatt gtaccgttat tggtaacatt gagctgtttc aaattactag
    17881 ataaaaaagt cggcgtcatc aaaattattt ttggaatggt cgtcttggga attttattgt
    17941 cattacttgg aatcgcatag taggaagctg cagctactag gctgcagttt ttttagcaag
    18001 gagggaaaag atgactatta atgaagtaat ttattcaccc aaacacccat tttttaatgc
    18061 tgagttaaac gacggaaatg tgactacttc aaacgaatac agctactatc ggaatattcc
    18121 tgatgtaaag ggattgacct tcgatgtaag taatcaaaag acattagcaa accttgatgt
    18181 ttgcgggacg attcaaacgt tgacctttta tcgagaaaat ttactaactg aggaaaaacc
    18241 aggtgtttgg gtcaataaac agctctctca aaccaattcg ttatccttaa aggttgaagt
    18301 aaacgggatt gtctaccatt tgtcagaatc ggatcatcgt attgaagtcg atgtgattca
    18361 agactgtttg cctagatata ttcatcatta ttcgacgttt aaggtcattg tagtatcctt
    18421 tgctcccata ctggctaata agcgtttaag tatgcttgtt caacaggttt acattgtgaa
    18481 tgaaacacac gaaagccttc aagtttcagt gaaagatgtg ccttcgtatc aagagaagta
    18541 ttcggatcag caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca
    18601 ggaagtggca agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac
    18661 ttttcaggaa tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct
    18721 atttgggcag ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta
    18781 tcaaagcttt tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg
    18841 cagctatcct gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt
    18901 caccatgttt gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt
    18961 taagtttcct ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc
    19021 cagtgcgtta ctggctagtc tttattttga acatactggg gatacaaaat tctttgagcg
    19081 gtgtccgcag gttctactga atgcttcaag aattattgag gacatattag ctcaacgtcg
    19141 gtcaggggaa ccaatgcttt ttccatctgt ttggttatca gacgcctttg cacttggaaa
    19201 gtatcatacc ggcagcaatc tttgtctttg gaaggcctgt attggacttg cagaaatttt
    19261 tgaagcccta gaacaactga gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa
    19321 ggaatcaatt tgtaaacaaa tgactatcga tggtcctttt ggtgagcaat ttttggaggg
    19381 aatcggtgat gaggagaaaa caacctattc agtgaagcac tatcaaaaac cgattcttga
    19441 gcaagggtta atctttttaa gtgatgtgat tgtggatgga aaaattaatt tattgatgca
    19501 tgatggagaa gaatcagaca ctacgttgat gcctttttat caatttttag ataataaaga
    19561 tcatttatat cagaacacca tggccttttc tgctagttct ttcaatccaa cctatagcga
    19621 ggaaatcaaa ggcatcactt ggggattaga atccggcgcc acttttcctg gctttatcac
    19681 tgttttaatg agcgagctgc acaaccctca agctttcgta acccgattag aagaactaat
    19741 ttgtttggca gatttagacg gttcgtggtg gtggtggccg tatcgcttag aggctcaaca
    19801 aggagaagtc gtgcgagact ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt
    19861 cagcctaatg atcagtcaat atttcggatt gaaattcaaa aaaggagtat tgcaaattga
    19921 tcctgttgat aatttaacgt tttcttggaa gaatttgaag ttcggtcaag ccttcatttc
    19981 gattgaatgt acgcaatccg agttgagtat ttcaaatttg tcagaaaagc cattgaaaat
    20041 aagtgatacg aaaaaaattc ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag
    20101 aaaaagatga gtatactaaa aaatattacc caatttaaaa aaatccagag tcgagcaatc
    20161 actcccgaaa atccaacagg cgagaaaggt aaagctgcga tgttgccaag cgagctagga
    20221 ccaagccgca aaggtcgtgg cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg
    20281 gatattactg gaacgggaat aattcgtcat atgtggatga cgattcgtga tcgaacagaa
    20341 tttggcagtt ttgttttacg tgatttaatc ttaagaattt attgggatgg gtcggatagc
    20401 cctgctgttg aaagtccttt aggggatttc ttttgtaatg ggtttggaga gcgttgcgag
    20461 gtgaactcgt tgccgatagt ggttaatcct acagggggct ttaattccta tttcgaaatg
    20521 cctttccaaa catccgctaa aattacgatc gagaatttac atccgaagga tatgtcctca
    20581 tttttttata cgataaatta cgctttggtg gacgaattgg aagaaaatag cctatatttt
    20641 cacgcaaatt ggcatcggga acgcattacc aaattacagg aggattataa gttaattgat
    20701 cgattaaagg gtcaaggcta ttacgtgggt acttaccttg cgcttacctg cctagaacgt
    20761 tattggtggg gcgaaggcga gtttaaattc tacattgatg gggatagtga gtatccgacc
    20821 gtaacctcaa caggttcaga agattatttt ggcggggcgt gggcctttca tgagtacgat
    20881 gagttcggac gtccacatgc gaaaaactat tcaacagcct ttttagggta tccttaccaa
    20941 tcaaatagag atgccacaag agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc
    21001 tttggtgacg acgcaatgcc acgacatggt ctttatcgtt ggcatcttca agaccccatc
    21061 tgttttaccg aagatttatc ggtcacttta caacaaatcg gcaacgatga tgttcgctta
    21121 tttgagcggc aagacgatgt tgctagtgtc gcttactggt attcaacaga agcttcagga
    21181 aatactaaac gtcatttcac tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac
    21241 atattcgaag ggaggaacct atgatgatat atggggcaat tgaagctgga gggactaaat
    21301 ttgtctgtgc agtcggtact gaggaattaa cgattttgga aagagtcagt tttcccacaa
    21361 caactccagt tgaaacaatg aagcaggtca ttgatttttt taaacctttt aaggaacaat
    21421 tggcaggcat cggggtcggc tgctttggtc caattgatat tcaatgcaaa tcaacgagtt
    21481 tcggtcatat cacatcaaca ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc
    21541 taaagcaata tttcaatatt ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg
    21601 gtgaatatgt tgctggttct ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa
    21661 caggtatcgg aggtggtgcc ttgttcaatg ggactttcgt ggaagggttc agtcatccgg
    21721 aaatggggca tatgttgatc aagcgacatc caaaggacaa tttttcagga aactgtccct
    21781 tccaccaaga ttgtttagag gggatggcag caggaccagc gattgaaaaa cggttgggtg
    21841 taaaaggcca gaatttattg gctgacgatt cgttttggca gattgaagcc ttttaccttg
    21901 cccaatgtgc atacaataca acactgatgt tttcgccaga tagaattatt tttggcggtg
    21961 gtgtaatgaa acaagaacac atgaagaaaa aagttcagga taaatttgtt gagcttataa
    22021 atggatatgt tgaaatacca ccaattgata gctacattat tacgcccgaa ttaggtgata
    22081 atgccggaat aatcggcgga ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat
    22141 aattaatgaa tgtttcattg atactcatgt caaaagtatt taaccgagta atcaaatgat
    22201 caaatgaagt tggataaagc gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg
    22261 ttgttttcat taaatcactg aaaggaaatg cttctgcgtt tccttttttc tttttgttgt
    22321 gcgctgtata ccttgaggat attcactggt ggcgtacact taaatagtaa tatattgtgt
    22381 tactttatgt ctgtggtaat agttatagtt gcttaccaat gtatgttcat ttttcgaaat
    22441 gttgacttca taatccttgg cttaatgttt gaattttttt ggtgatagat tttttcagcc
    22501 gtataaaata aatcgtaatt ctattataga gagttgtgat tataaaaaga tagcaagaaa
    22561 aaaaaagaaa gtttctatac attttaaata ttttattcct at
//
LOCUS       DAYYOA010000036        22031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000036 DAYYOA010000000
VERSION     DAYYOA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.036"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACHBKA_001246"
     CDS             367..555
                     /locus_tag="ACHBKA_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444945.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACHBKA_001247"
     CDS             545..805
                     /locus_tag="ACHBKA_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFM9444946.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACHBKA_001248"
     CDS             846..1073
                     /locus_tag="ACHBKA_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444947.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACHBKA_001249"
     CDS             1138..1710
                     /locus_tag="ACHBKA_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444948.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACHBKA_001250"
     CDS             1711..2022
                     /locus_tag="ACHBKA_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9444949.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACHBKA_001251"
     CDS             2013..2273
                     /locus_tag="ACHBKA_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444950.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACHBKA_001252"
     CDS             2434..2793
                     /locus_tag="ACHBKA_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444951.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACHBKA_001253"
     CDS             2790..4433
                     /locus_tag="ACHBKA_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9444952.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACHBKA_001254"
     CDS             4468..5550
                     /locus_tag="ACHBKA_001254"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFM9444953.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACHBKA_001255"
     CDS             5594..6766
                     /locus_tag="ACHBKA_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9444954.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACHBKA_001256"
     CDS             6753..7457
                     /locus_tag="ACHBKA_001256"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9444955.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACHBKA_001257"
     CDS             7462..8622
                     /locus_tag="ACHBKA_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9444956.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACHBKA_001258"
     CDS             8698..8976
                     /locus_tag="ACHBKA_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFM9444957.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACHBKA_001259"
     CDS             8957..9328
                     /locus_tag="ACHBKA_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFM9444958.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACHBKA_001260"
     CDS             9344..9748
                     /locus_tag="ACHBKA_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444959.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACHBKA_001261"
     CDS             9745..10155
                     /locus_tag="ACHBKA_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444960.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACHBKA_001262"
     CDS             10216..10803
                     /locus_tag="ACHBKA_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9444961.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACHBKA_001263"
     CDS             10876..11343
                     /locus_tag="ACHBKA_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFM9444962.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACHBKA_001264"
     CDS             11389..11697
                     /locus_tag="ACHBKA_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444963.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACHBKA_001265"
     CDS             11892..18626
                     /locus_tag="ACHBKA_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9444964.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18657..19502
                     /locus_tag="ACHBKA_001266"
     CDS             18657..19502
                     /locus_tag="ACHBKA_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM9444965.1"
                     /translation="MNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESIEL
                     SEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTEID
                     SQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYTKNVWEKKGV
                     FKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFINMKDTQILE
                     VSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGHANVEIELYE
                     ERDLF"
     gene            19499..20548
                     /locus_tag="ACHBKA_001267"
     CDS             19499..20548
                     /locus_tag="ACHBKA_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444966.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACHBKA_001268"
     CDS             20552..21376
                     /locus_tag="ACHBKA_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444967.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACHBKA_001269"
     CDS             21394..>22031
                     /locus_tag="ACHBKA_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM9444968.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAYYOA010000037        21988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000037 DAYYOA010000000
VERSION     DAYYOA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.037"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHBKA_001270"
     CDS             <1..395
                     /locus_tag="ACHBKA_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9444969.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACHBKA_001271"
     CDS             398..1147
                     /locus_tag="ACHBKA_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9444970.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACHBKA_001272"
     CDS             1159..1428
                     /locus_tag="ACHBKA_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444971.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACHBKA_001273"
     CDS             1433..1597
                     /locus_tag="ACHBKA_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444972.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACHBKA_001274"
     CDS             1590..1892
                     /locus_tag="ACHBKA_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444973.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACHBKA_001275"
     CDS             1889..2050
                     /locus_tag="ACHBKA_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9444974.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACHBKA_001276"
     CDS             2047..2352
                     /locus_tag="ACHBKA_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444975.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACHBKA_001277"
     CDS             2352..2708
                     /locus_tag="ACHBKA_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM9444976.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACHBKA_001278"
     CDS             2668..2913
                     /locus_tag="ACHBKA_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444977.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACHBKA_001279"
     CDS             2910..3329
                     /locus_tag="ACHBKA_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM9444978.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACHBKA_001280"
     CDS             3326..3883
                     /locus_tag="ACHBKA_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM9444979.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACHBKA_001281"
     CDS             3880..4176
                     /locus_tag="ACHBKA_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444980.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACHBKA_001282"
     CDS             4253..4666
                     /locus_tag="ACHBKA_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9444981.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACHBKA_001283"
     CDS             5012..5386
                     /locus_tag="ACHBKA_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444982.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACHBKA_001284"
     CDS             5423..5590
                     /locus_tag="ACHBKA_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444983.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACHBKA_001285"
     CDS             5616..5960
                     /locus_tag="ACHBKA_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM9444984.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACHBKA_001286"
     CDS             5965..6246
                     /locus_tag="ACHBKA_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444985.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACHBKA_001287"
     CDS             6349..6663
                     /locus_tag="ACHBKA_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9444986.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACHBKA_001288"
     CDS             6641..8335
                     /locus_tag="ACHBKA_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9444987.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACHBKA_001289"
     CDS             8355..9533
                     /locus_tag="ACHBKA_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9444988.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACHBKA_001290"
     CDS             9496..10182
                     /locus_tag="ACHBKA_001290"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9444989.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACHBKA_001291"
     CDS             10182..11342
                     /locus_tag="ACHBKA_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9444990.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACHBKA_001292"
     CDS             11352..12227
                     /locus_tag="ACHBKA_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444991.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACHBKA_001293"
     CDS             12224..12535
                     /locus_tag="ACHBKA_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444992.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACHBKA_001294"
     CDS             12525..12878
                     /locus_tag="ACHBKA_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9444993.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACHBKA_001295"
     CDS             12868..13269
                     /locus_tag="ACHBKA_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444994.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACHBKA_001296"
     CDS             13262..13666
                     /locus_tag="ACHBKA_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444995.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACHBKA_001297"
     CDS             13678..14286
                     /locus_tag="ACHBKA_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9444996.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACHBKA_001298"
     CDS             14306..14668
                     /locus_tag="ACHBKA_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444997.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACHBKA_001299"
     CDS             14671..14853
                     /locus_tag="ACHBKA_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9444998.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACHBKA_001300"
     CDS             14870..18289
                     /locus_tag="ACHBKA_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9444999.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACHBKA_001301"
     CDS             18340..19077
                     /locus_tag="ACHBKA_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9445000.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACHBKA_001302"
     CDS             19087..21378
                     /locus_tag="ACHBKA_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9445001.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACHBKA_001303"
     CDS             21402..>21988
                     /locus_tag="ACHBKA_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM9445002.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAYYOA010000038        21912 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000038 DAYYOA010000000
VERSION     DAYYOA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.038"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHBKA_001304"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHBKA_001304"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM9445003.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHBKA_001305"
     CDS             complement(1172..1867)
                     /locus_tag="ACHBKA_001305"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM9445004.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHBKA_001306"
     CDS             complement(1880..2596)
                     /locus_tag="ACHBKA_001306"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM9445005.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHBKA_001307"
     CDS             2926..3534
                     /locus_tag="ACHBKA_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM9445006.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHBKA_001308"
     CDS             3859..4182
                     /locus_tag="ACHBKA_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9445007.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHBKA_001309"
     CDS             4364..5734
                     /locus_tag="ACHBKA_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM9445008.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHBKA_001310"
     CDS             complement(5864..7165)
                     /locus_tag="ACHBKA_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9445009.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHBKA_001311"
     CDS             complement(7519..9510)
                     /locus_tag="ACHBKA_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM9445010.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHBKA_001312"
     CDS             complement(9654..9920)
                     /locus_tag="ACHBKA_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9445011.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHBKA_001313"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHBKA_001313"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM9445012.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHBKA_001314"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHBKA_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM9445013.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHBKA_001315"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHBKA_001315"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9445014.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHBKA_001316"
     CDS             complement(14080..14601)
                     /locus_tag="ACHBKA_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM9445015.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHBKA_001317"
     CDS             complement(14604..15446)
                     /locus_tag="ACHBKA_001317"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM9445016.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHBKA_001318"
     CDS             complement(15439..16164)
                     /locus_tag="ACHBKA_001318"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM9445017.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHBKA_001319"
     CDS             16340..16636
                     /locus_tag="ACHBKA_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445018.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHBKA_001320"
     CDS             17204..18796
                     /locus_tag="ACHBKA_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9445019.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHBKA_001321"
     CDS             18809..19549
                     /locus_tag="ACHBKA_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9445020.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACHBKA_001322"
     CDS             19717..21066
                     /locus_tag="ACHBKA_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9445021.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACHBKA_001323"
     CDS             complement(21194..21784)
                     /locus_tag="ACHBKA_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9445022.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DAYYOA010000039        21825 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000039 DAYYOA010000000
VERSION     DAYYOA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.039"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1159
                     /locus_tag="ACHBKA_001324"
     CDS             <1..1159
                     /locus_tag="ACHBKA_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9445023.1"
                     /translation="ANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKRAEKI
                     RDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHNRIYK
                     FFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELISTNV
                     ADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSA
                     IDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQD
                     NRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNAS
                     IMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1733..2302
                     /locus_tag="ACHBKA_001325"
     CDS             1733..2302
                     /locus_tag="ACHBKA_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM9445024.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2289..3149
                     /locus_tag="ACHBKA_001326"
     CDS             2289..3149
                     /locus_tag="ACHBKA_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9445025.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3339..4043
                     /gene="yycF"
                     /locus_tag="ACHBKA_001327"
     CDS             3339..4043
                     /gene="yycF"
                     /locus_tag="ACHBKA_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM9445026.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4048..5883
                     /gene="walK"
                     /locus_tag="ACHBKA_001328"
     CDS             4048..5883
                     /gene="walK"
                     /locus_tag="ACHBKA_001328"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM9445027.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5880..7196
                     /gene="yycH"
                     /locus_tag="ACHBKA_001329"
     CDS             5880..7196
                     /gene="yycH"
                     /locus_tag="ACHBKA_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM9445028.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7197..8066
                     /locus_tag="ACHBKA_001330"
     CDS             7197..8066
                     /locus_tag="ACHBKA_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM9445029.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8121..8930
                     /locus_tag="ACHBKA_001331"
     CDS             8121..8930
                     /locus_tag="ACHBKA_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9445030.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9033..9662)
                     /locus_tag="ACHBKA_001332"
     CDS             complement(9033..9662)
                     /locus_tag="ACHBKA_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9445031.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9832..11124
                     /locus_tag="ACHBKA_001333"
     CDS             9832..11124
                     /locus_tag="ACHBKA_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9445032.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11200..11862)
                     /locus_tag="ACHBKA_001334"
     CDS             complement(11200..11862)
                     /locus_tag="ACHBKA_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9445033.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12087..12371
                     /gene="groES"
                     /locus_tag="ACHBKA_001335"
     CDS             12087..12371
                     /gene="groES"
                     /locus_tag="ACHBKA_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM9445034.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12422..14047
                     /gene="groL"
                     /locus_tag="ACHBKA_001336"
     CDS             12422..14047
                     /gene="groL"
                     /locus_tag="ACHBKA_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM9445035.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(14136..15275)
                     /locus_tag="ACHBKA_001337"
     CDS             complement(14136..15275)
                     /locus_tag="ACHBKA_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9445036.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15386..15970)
                     /locus_tag="ACHBKA_001338"
     CDS             complement(15386..15970)
                     /locus_tag="ACHBKA_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM9445037.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(16097..16528)
                     /locus_tag="ACHBKA_001339"
     CDS             complement(16097..16528)
                     /locus_tag="ACHBKA_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9445038.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16546..16866)
                     /locus_tag="ACHBKA_001340"
     CDS             complement(16546..16866)
                     /locus_tag="ACHBKA_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9445039.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            17166..17306
                     /locus_tag="ACHBKA_001341"
     CDS             17166..17306
                     /locus_tag="ACHBKA_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445040.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            17604..17861
                     /locus_tag="ACHBKA_001342"
     CDS             17604..17861
                     /locus_tag="ACHBKA_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFM9445041.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17832..18140)
                     /locus_tag="ACHBKA_001343"
     CDS             complement(17832..18140)
                     /locus_tag="ACHBKA_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445042.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            18218..18964
                     /locus_tag="ACHBKA_001344"
     CDS             18218..18964
                     /locus_tag="ACHBKA_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM9445043.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            18979..19182
                     /locus_tag="ACHBKA_001345"
     CDS             18979..19182
                     /locus_tag="ACHBKA_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445044.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            19287..19586
                     /locus_tag="ACHBKA_001346"
     CDS             19287..19586
                     /locus_tag="ACHBKA_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445045.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            19586..19768
                     /locus_tag="ACHBKA_001347"
     CDS             19586..19768
                     /locus_tag="ACHBKA_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445046.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19774..20250)
                     /locus_tag="ACHBKA_001348"
     CDS             complement(19774..20250)
                     /locus_tag="ACHBKA_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445047.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            20303..21028
                     /locus_tag="ACHBKA_001349"
     CDS             20303..21028
                     /locus_tag="ACHBKA_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM9445048.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            21202..21543
                     /locus_tag="ACHBKA_001350"
     CDS             21202..21543
                     /locus_tag="ACHBKA_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445049.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            21536..>21825
                     /locus_tag="ACHBKA_001351"
     CDS             21536..>21825
                     /locus_tag="ACHBKA_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9445050.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         7424 a         3632 c         4367 g         6402 t
ORIGIN      
        1 agcaaacata attgaaaaaa gaggaacatt acaagtagtt gtatcttata aagactctaa
       61 cggaaaaatt agacaaaagt ggaaagatac aaaattaaaa aagaaaggaa ataaaaaacg
      121 agcagaaaaa attagagatg aatttttact agaattggaa agtcaattag ctatggaatg
      181 tgaatgtgct aatccaaatt tattgtttta tgactatttg cttaattatt tggaagtatc
      241 aaaaaaacaa gtagcattta acacatatgt tgggtataag cattacgtcc ataatcgaat
      301 ttacaagttt ttttatccta aaaaaattaa actaaatagg ttaaagcctt atcatttaca
      361 agagttttat caatatatgt taaatgataa ctgtacagca aatacggtta ttcattatca
      421 cgcatttata cgcaaagctc ttcaagaggc tgttattaca gaattgataa gtacaaatgt
      481 agcagataaa gtaagaaagc caaaaaagaa acaatttgtt tctcaagttt ataatcaaca
      541 agagatcttg aaattacttg acattttgtc agaagaaaaa ctctatttgg tagttctttt
      601 aacagcattt tatggattgc gaagaagtga agttttagga ttaaaatggt ctgctattga
      661 ttttttaaac aaaaaaatca tgataaacca tgtaatcata gaaaatccag agaatatgtc
      721 acagttaata aagaaagatc aaacaaagaa ctcttccagc tatcgtacat tacctttggt
      781 tgaaacgatt gaacgagcat tagtaaatca agctaaatgg caacaagata atcgtattct
      841 tttaggagat gattatattt tagaagataa tgagtacgta tttactatgg aagatggacg
      901 gttgatgaag cctcaatatg taacgcatcg tttgagtaag ttgattaaga aaaatggatt
      961 gaaaaaaatt cgctttcatg acttgcgtca ttctaatgct tcaataatgt tagatagtgg
     1021 ccagaatatg aaatccattc aagaatggct gggacatgca tcgtattcga caactgctaa
     1081 tttatacacg catttaaccg caggagtaaa agatcgtgca gcagaagcat tagaaaacgc
     1141 atttgggttt aaagaataaa aaaacaaaaa acaatatctt tggggtatcg cttcttgttt
     1201 ttagaatttt tttgttggca atttgttggc agtatgttgg caatgaaaaa attcacaaac
     1261 taaaaaccta tttataaagc tttataaaac ttttttcaca aatagagcat ttggttaaga
     1321 gtcagctgct ctaccaactg agctaacggc ccgtacaaaa agtacttaat aatagtagca
     1381 ctaagaattt taaaatgcaa gactacattt aaagaaaatc agaaaaatct ttacttcctt
     1441 ataggaaatc attcatagat tattcacatt tgctcgctat tatataagca taccaacaaa
     1501 caaagttctt gcaagagaac aaaccctaac acttttttca ttttttactc ctttcccgcc
     1561 gtctccccgg cgggtctcct gttcttaacg ataaaagaca agaaggctta gccttcttgt
     1621 tttttgtttt catggaatcc cgctaatcaa atcacctagt taaagcaact agattgaatg
     1681 tttatcataa gtctgatatc atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa
     1741 aatgcgtcag caattaatgg agtgggggaa ggaactagaa acattccgtc tgcctcactg
     1801 ggaagaactc cctgacttag aactttatat ggaccaagtc agaacgttag ttgatcgtta
     1861 tctttcacct gtgatacaag gagaaaagca tccattgctt acctcttcta tggtaaacaa
     1921 ttacgttaaa ctaggtttga taccagcacc agttaaaaaa agatataaca aagaacacgt
     1981 agcattttta cttgcaatca ctacgttgaa acaagtattg actattcctg aaatcaaaga
     2041 agggatattg ttccaaggaa aaacagtagg aattcgtgaa gcctataatc tattttgcga
     2101 tgaacaagaa gcagctgtgt ggatggtcag ccaattagca caaggtaaat ctcatccgca
     2161 aaaattcgac caggcaaccc ctgtagaata tattgctgta agagcggcaa ctttatcgtt
     2221 tgcaatgaaa cttcttgcgg aaaaaacgat tgttctagaa accgaatatt taaaagagga
     2281 gaaaacaaat gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg gatgttccag
     2341 aagaattgat aaaaaaatac gagatgtata tgatcccatt gaaaattatt tataaagatc
     2401 aggtatatac agataaaatt gacattaccc ctgaagagat ctatcaacgt ttgccagagg
     2461 aaatccctgg tacatctttg ccggatggtg agtcgatcac agaaatcttt gagcaaatca
     2521 aaaaagacgg ttatgaaaag gtacttgcgg taacaatttc cagtggattg agcgggacat
     2581 ataatgtggt tcgtttaatt gcacaacagc aagaagaatt ggaagcatat gtactagata
     2641 cgaaaaatat tggtattggt gcaggttttt ctgcgatcca agcagcaaaa tggctggaag
     2701 aaggagtaga atgggatcaa ctgatcagta atctgaatga gttagtcgaa agaacaaaag
     2761 ttttctttaa tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc ggtttagttg
     2821 cttctattgt aggaaccgct ttaaagctta acccaatcat ctcatgtaat gaagaaggga
     2881 tttattacac agtaggaaaa gctaggggac gtaaaaaaag tttggataaa acggttgctt
     2941 acgtcaaaga aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc catggggatg
     3001 cgaaagaaga agcaattgaa atggaagcga gacttaaaag agagttccca caagcaaacc
     3061 aaatctactt tggccagatt tctccggcat tagtagtcca cactggtccg ggattattgg
     3121 gtgtcggtgt acaagtttta gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc
     3181 agtgttttcc ttttttattg aagttagaac aaaaaataat aaagtatttt gcactttcat
     3241 tttaattttc tcttttgttt tataggcaaa gatgaaacaa aagcagaaat acggtataat
     3301 gagaaggtat gacaataata tataagaggt ggctctttat gaaaaaagtt ttagttgtcg
     3361 atgatgagaa gccgatttcg gatatcgtca aattcaattt agccaaagaa ggctatgacg
     3421 tttatactgc atatgatggg gaagaagcct tggaaaaagt tgctgaggta gaacctgatt
     3481 tgattttact tgatttgatg ctacctaaaa tggacggctt agaagttgct cgtgaggtac
     3541 gcaaaactta tgatatgccg atcattatgg taacagccaa agattctgaa attgataaag
     3601 tcctaggctt agagttaggc gccgatgatt acgtgaccaa gccattttcc aaccgagaat
     3661 tagttgcacg tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag
     3721 aggcagcacc tgcggaattg acgattggtg atttgacgat ccatccagaa gcttacatgg
     3781 tgacgaaacg aggagaaaca atcgagttaa cacatcgtga attcgaacta ctcttttact
     3841 tggcgaagca ccttggacaa gtgatgacaa gagaacattt attacaaacg gtttggggtt
     3901 atgattattt tggagatgta cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga
     3961 tcgaagataa tccaagccat ccaaattatt tagtgacacg tcgtggtgtc ggttactatt
     4021 tgcgtaaccc agaacaggag taattggatg aaaagaaaag tccgcttttt tcgatcagtc
     4081 aattttaaga tcgctattac ctttattctg attttattga tctcgatcga aattattggt
     4141 gcctacttta tacgtggttt agaacgttcc acgatcaata catttattaa agatatgaat
     4201 caaacggtgg aatcattagc aacaacaatc agcccggaat tgaatcgaaa agataatgca
     4261 gatgatgaag aggtaaatgc gaatatcaaa agatttattg aaaacagtgc gactagcgat
     4321 atcatcgaaa tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat
     4381 gaacaaagcg ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt cacgactaaa
     4441 cgttatgttg ctttagataa cgacgataaa cgggtgaata tcaatgttca accgatcctg
     4501 tccccgaccg gcgatgccgt tatcggggct ctttacgtca aaagtaatat cgaacaaaaa
     4561 tattcggaaa tcaataacac agcagtcatt ttctttactg cctcactaat tgcggcattt
     4621 atttcaatga tcgtatctgt tttagttgct cgttcgatta cacagccgat cggtgaaatg
     4681 agagaacaag ccattcgaat cgctagagga gactacagtc ggaaagtcaa agtgcatgga
     4741 caagatgaac ttggacagct ggctgatacc ttcaatcagt tagctgaacg tattgaagaa
     4801 gctcaagata caatggaagc tgaacgcaat cgtttggata gcgtgctatc ccatatgaca
     4861 gacggcgtca ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga tatggctgtt
     4921 tccttgctag atatcaaaaa cgaggaagca atcggacaat cgattttgac acttttagat
     4981 atcgaagaag aatatacgtt gcggaaatta ttagaagatc caaacgaaat ggtacttgac
     5041 cgttcaacca gtacattgga aagtgatcag atgatcttgc gagtagactt ttctatgatc
     5101 cgtcgcgaat caggttttat cagcggatta gtagctgtct tgcatgatgt gacagaacaa
     5161 gaaaaaacag aacgggaacg tcgtgaattc gtctctaatg tttcccatga acttcgtaca
     5221 ccgttgacaa gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag
     5281 gaagtagcac caaatttttt gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg
     5341 atcaatgacc tcttaaatct atctcgcatg gatacaggta atacccagct tcaattagaa
     5401 tatgtcaatt tcaacgagtt agtgaatttt gttcttgacc ggtttgatat gatggtggga
     5461 aatcaggaaa aaaattataa gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa
     5521 gtagatacag ataaaatcat ccaagtagtc gataatatca tgaataacgc gatcaagtat
     5581 tcaccagatg ggggaacgat tacttgtcgc ttattagaaa cgcataataa cgtgatttta
     5641 agtatcaccg atcaaggatt ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg
     5701 ttctatcgag tagataaagc acgtgcaaga gcacaaggtg ggacaggact tggattagct
     5761 atttctagag aagtgattaa ggcgcatcga ggcgctattt gggcagagag caaggaagga
     5821 aaagggtcga ctttttatat ctcgctgcca tatgaacctt atgaggagga atggtgggaa
     5881 tgagaatctc tgaaaaagtc gttcgtatag gactcatttt gatggttgcg ttaagcatat
     5941 acttctctta tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac gtagatgaga
     6001 caaacactca aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc
     6061 ttcatgttac atggcaccat tcgggaatga tccaagaaac caacagtgaa aatctcgtgt
     6121 accgattgca attgatcatc gataaagcaa gatttggaaa gctttctgaa gtagtttcag
     6181 gagacaaatc acagtttgaa caggtgaaaa atctgaactc tggttttgag ttggcctata
     6241 atgcgccttt tttgctaaga gaatacaagg aagcatttga tttagattta gatgtgtcga
     6301 acttggcaaa agaaagcgaa aatatgtatt ttactagaat acaattcgat caaggaaata
     6361 aaaaaatccg atttttgaat tattcgaacc atctcgtata tgaagcaaat atttcgttga
     6421 attggacaga tatcaataaa gaattaacag caaaagacac aaaatggaca gaaatgacaa
     6481 cagaaccatc gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa ctgaagaagt
     6541 atagttatat cctgtcacag caaccctata cactttttag aaatgctttt ttccagaaac
     6601 cagatagcgt gaaaaataat gatgaaagca atgaattgat tttttacggt gggaatgaaa
     6661 caatgtctat ccattcggaa acgcaagtag ttgattttcg tggagagctg ccagagaaaa
     6721 aagaaaatgc tagtgtattt tctgaaagtt tcccctatat tagcaaatta ggcagttctt
     6781 tagggaacct cagatatttt gaccgcagtg acaatacgat cgattatcgg atattcgtgg
     6841 aaggcttccc ggtttttggg acagacagca gaggacagat tggtgtggaa gttggcggtg
     6901 aaacgaatgg ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt caagtgccga
     6961 taccttcaga cgaagaagta gaacttgcaa gtacgaatga aatcatccag cagttaaaag
     7021 ctaagggagc agatagcaac aagattggtt cagtgatcgt tggctataca tggcaaaatg
     7081 tcaaagaaac gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa tataaagata
     7141 actggtattc agctaacgac cttctggaaa aattacctgg attggaggcg aattaattgg
     7201 atttcaaaaa aatcgaatgg atatttttcg tcgctttttt agggttgaat gtgtttttat
     7261 tcagcagtta tcatgacgcg gtataccaag aaaacaatgt cattcgctct aatcagactg
     7321 aatcaatcca acaacgtctg gctagcgacg atatcactta cacggggaag atttcgtctg
     7381 agaacaagca aggctattat cttagcgcgg agcagaccaa tcttagcact tcattagcga
     7441 attatcgcaa ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta gacggtgatg
     7501 ttttgacgaa atctttatcc agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa
     7561 ttagtagttc cttggaagat tttttatcta acaaagacaa tattttattc ggtaatgatt
     7621 atgtgtacac gaaaaacttt tctcatttag aagaagaacc actagaaatc caagcaagcc
     7681 aagagtatga aggcatccca ttcaatgatg atacagcaaa aatcgttctt tctttagaaa
     7741 aaaacgataa tgattatagc atcacgaaat acatgcagac gcatttatct gatatcgagc
     7801 agctgcgcga aaagacggaa ctgtatacgg aagaggaagc aatcaataca ttgtacatca
     7861 ataacaaaat accaagagga tcaaaaattt tatggcgtca gctagcttat tcctgtattc
     7921 tgaaagtccg ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg
     7981 ataaagtggt ccaaattgag agcgtcaacg ctttcagcaa tacgatcgtg acaaacaata
     8041 ctgtaccaaa agtggaagat caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta
     8101 agaagggaag taggagtcaa atggctgaag aaaaaccagc ttttaaaatc agtatcctag
     8161 caagtggaag ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg cttttggtcg
     8221 atgcaggact tagcggtaaa aaaatcactt ccttgatggc tcaaattgat cggacaccgg
     8281 accaattaga tggaatactt gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg
     8341 tcttggctcg taagtatcat ttggatgttt ttgccaatca gcagacttgg gaagcgatgg
     8401 aaccactgat tggaaatgta ccagtggaac aaaaacatct atttgagatg gggaaagtca
     8461 gaacattcgg cgatatcgat attgagagtt tcggcgtctc ccatgacgca gcggctccgc
     8521 aattttatcg tttccataaa gaaaatcggt cattcgtcat gttgacggac accggatatt
     8581 gcagtgatta tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc
     8641 atgatcttga gatgttacgc atgggacctt atccgtggaa tttaaaacag cgtattttag
     8701 gagatagagg acatctttcc aacgaagacg gtgcacttgt catgactgat gtgatcggta
     8761 atcggaccaa acgaatctat cttggccatc ttagcaagga aaacaatatg aaagaacttg
     8821 ctcatttaac aatggaaaat gttttgaaag aaaaagattt tggcgttgga catgattttg
     8881 aaatctatga tacagatcca gatgaagcaa ctgagctatt ttcgatttaa acagttcaat
     8941 caataaaaat ataaaaagca gaagataaaa tcaatcaggg ttggttttat cttctatttt
     9001 tattttttta gcatattaaa aaaataaccg tattatcgac tgactacact gatgtgctgc
     9061 ttttcatgtt tctcatccag tatctcatct actaatgcaa tcgcgtaatc tgccatgctg
     9121 atcgtactgt cgctagcagc gtttctttgt aaataatcat cacttaacaa ataagtacct
     9181 gtacgtttgc catgtggatc aaataaagaa gctggactga tataagtcca atgtagattt
     9241 ttagattgtt tcaattttaa caatccttcg tacatattga aagctgttgg atagaagggt
     9301 gcatttttag gtacttggtc aatcatacga gtcgtctttt ctgtatctaa gaataacgaa
     9361 gaggcacccc caacgacaat taagcgtgta tcggttcctt caagcatctc aatcaaatgg
     9421 gacaaagaag tttgatgcat ttcttctttt cctttgggag cgttgaaagc atcaacgaca
     9481 acatcaaaag atgcaagatc agtagttgtc aactcaaata aatccttttg tataaacgga
     9541 acatctgatg gcaatttttc tggatgacgc acgattgccg tcacgtctaa atctctggac
     9601 aatgcttcgt ctaaaataaa tgaacctgca tggccagtag ctccaatgag tgcaattttc
     9661 ataaaaaacc tcctttttta ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag
     9721 aatccgattt ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc attgggtata
     9781 gttaaattaa gaaaactgat tgataaaaat agaagttgaa ggagataaga aatgaaagag
     9841 tggttagatc gtgccttttt accaatgata acatctgttg tgaatcttag acccttaact
     9901 gctttgcgta atggaatgac ttacacgatc ccaataatta ttgttggctc gctgtttttc
     9961 ttatttgctc attttcctgt ggattacatc gctcagtgga tagacaaaac aggattttct
    10021 atgtatttta cacaagtata ccaggctaca tttgatgtga tagccttttg gatcgttttt
    10081 gccattgcat acgcttatgc aaaagaagaa gacatcgatc gcttaccagt cggcataaca
    10141 gccttgtctg cttatctact gcttattcag ccactaaata ctcagataga tactgtctgg
    10201 acaggtgctg aagggatact aggagcagtt acaactgggc tcatcatcgg atggggctat
    10261 gcacgtttag taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt
    10321 tggcagtctt ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt
    10381 tcagcggttt ttcaacattt gttcagaatg actgttgttc aaggagtatg gacatatgtc
    10441 cagcttccca ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt
    10501 gttccgtttt tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt
    10561 cccgtcttgc aagcgaattc attagcaaat gcagaaatct tagcctcagg acaagagttg
    10621 acggtggcaa atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt
    10681 acgggtgctg gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag
    10741 aaatacagag aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca
    10801 attatttttt ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca
    10861 ccggtcgttt caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt
    10921 acagcagtcc aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga
    10981 gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca
    11041 tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga
    11101 gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa
    11161 atcaccaatc taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac
    11221 agctaattga acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca
    11281 gatccgacca gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag
    11341 cagatgacca tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca
    11401 aaaatttgaa tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat
    11461 gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat
    11521 gatattttgt gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac
    11581 agcgatgctt tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt
    11641 tggagcagaa gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata
    11701 aaaaatagct aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc
    11761 agacgtagat tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc
    11821 atagcaaaaa atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag
    11881 ggataacaag catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa
    11941 taagcagctg tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga
    12001 gaatgagtat tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa
    12061 aaaaagagtt atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg
    12121 aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag
    12181 agaaacccca aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg
    12241 aaaaagttcc tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta
    12301 cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga
    12361 ttgttgagta aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat
    12421 catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga
    12481 taaattagca gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga
    12541 aaaatcttac ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga
    12601 attagaagat cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac
    12661 aaatgatatt gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg
    12721 cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga
    12781 attggcaaca aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa
    12841 agaagccatt gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat
    12901 tgctgatgca atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg
    12961 gatcgaaaca gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca
    13021 atatatggta acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat
    13081 cacagacaaa aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca
    13141 acaatcacgt ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct
    13201 tgtattgaac aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg
    13261 cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac
    13321 tgacgatcta ggtttagaat tgaaagatgc aacaatcgaa aacttaggaa atgcttctaa
    13381 agttgttgta gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat
    13441 cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg
    13501 cgaaaaatta caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg
    13561 tgcagctact gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc
    13621 tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt
    13681 tatcagcaaa gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt
    13741 tgttcgtgct ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc
    13801 agtgattgtt gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg
    13861 agaatgggta aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc
    13921 cttgcaaaat gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga
    13981 taaaccagaa ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggtat
    14041 gatgtagtaa ttaagaaaga accctatttt aacagggttc tttcttcttt ttttgtttta
    14101 ttttaagcaa aaggggcaaa gaaggggcaa aaatatcaaa ggctatttag tttatcaatt
    14161 acttgttgtt tggctctttt tgttacatgg ttataaatag acagagttgt gtttgcatca
    14221 gaatgcccta cacgctccat aatggcttta agaggtacgc ctaattcaga taatagtgaa
    14281 acgtggctat gtctgaatat atgtgaggat agactttttt ctaattccaa ctcttcctct
    14341 actttatgga gtatagcgtt aaatgagtgc agcgcaagcg gagtgccact tgtagatata
    14401 aatatatatt gatcgggatc tgtgggtcta cctgcaagaa tattgtcagc tatcacactt
    14461 tcaatcaatt cttttgcacg attgggtaat tgcacttcac gttgcgaata agtatttttt
    14521 ggagttgttt ttatagcatt atccattttc acagatgtgt aatctaaggt cccattaatg
    14581 gaaattttcc catcctcata gtccttcatt tgcaaagcta gcaattcccc atatctcaaa
    14641 ccagttaaat atagaaactc agctattatg ccgtgtagtt ttctgcgagg attggagtat
    14701 agctgtttca gtatttgatc aatttcttct ttctccagat attttttatt catagaaagc
    14761 cttctttttt cttcttccac ttttttagga tggattttaa ctgctagcgc agggtttctt
    14821 tgaatgtatt ttctatcgat tgcatagttt agcataacag atagagttgt ttttgtttgt
    14881 tttgtgtagt tcagtgagag gtcaccaaac gtatacatat cttcaattat cttattaatg
    14941 agtgtctcat caatgtttct aacgatcgta tcatcgctta tgtgcttaga aacatgtttc
    15001 atcatcattg gaaccttcaa atagctagta cgtttaacat gctgcttata atattcatac
    15061 cattctttat acagctcacc aaaagtgata tctgatttat tgtaatcttc gagtgcttct
    15121 ttaatttttt tatctagaat tttctgagct tttttccacg cttgtggtga attacttgta
    15181 agtgttgtag atttttttcg tgttttttct gtataaggat ctacatatct ttcaataaac
    15241 ttgaattggc catctttggt ctgttcaacc cacactttta acatctccta tcatttgcta
    15301 taataggcat aacaaataga cctatatagg tttgttttct aaaagcacgc tcttactctg
    15361 gacggtgggg cgtgtttttt atttattata cagttgcttt aaaaatagct gctttttcaa
    15421 atgatactaa aggagagaag tggatttcta tttctccctt ttggtttaac gtgaaatgag
    15481 cagttacatc catttgtttt cctggagcaa cagatcccat tgtattttcg ttggcatatg
    15541 tctctgattt tttgtcatct ggtccatata cttccacatc tgtacctaca gggatatctg
    15601 aatcgccatc attttttacg gtataagtaa tttttactac ttgtgcaggt tggttttctt
    15661 caaattgatt tctttcatca gttagttcta cactatttag tgtatattca gcatcaccaa
    15721 cttttacagt gtcaccaatc ttatagaagg tatcgctttt ttcttcttta gaagatgaag
    15781 cagaggttga agatttagtt actttttcgc caccattatc acttgcttta ttactatctg
    15841 atccaccatt taaagcagaa ccaataatta taattaaaat tactgctaat acccaaaacc
    15901 atactttttt gtaaaaaggt ttactaactt tatacatttt tccgtcttga cccataactt
    15961 tttttgccat ttaaatattc ctcacttctt gttataatat atttgcgatc tcagaaatga
    16021 ggtatgagtc cgtgtgccag cacgggcttt tttaatgttt tgctgaaatc ggttttttta
    16081 ataattcttg atgtattcat acatattgcc ttgcgtaaga atattttttc ttaaaatagc
    16141 attggcagac agcataacaa gtgtatctga gcttattctg aatagaataa tactccataa
    16201 atttttcgag attaaactga gattcatcag tcagttcgtt ctcaatatag atatttaata
    16261 aaattagaat agctatttta tctgcttctg tttcaaattt tgagtgaaaa gttgtagaag
    16321 tatcgtacaa aactgaatat tcaaaatgag aagcggtgaa atgcgcaagt tcatgagata
    16381 aatgaaaggc ttctgcagtt tcgccatata gattttcatt caaaaaaatg attctaggct
    16441 tcggataata gaagccaggc tctttcattt ccatgtaaac tactttcaaa ttgtattcgc
    16501 tcaacatttc tttcaatttc aaatacatac aacccatcac tccaactatt cattttcctc
    16561 taaagcttta gcaattgcaa tcgctttacg cattgtctcc ttagatattt cttttccatc
    16621 aaaagaaaaa acagtatcgt tttctgataa atccacatgt ttaggggttt ctctttcttc
    16681 tctacctaga aggtagtcta cagagactcc aaagtaatca gctaaccgag ctaactcaat
    16741 agaattagga gtttgtcttt tccaacttcc taaatatcca ttcgaatacc cgaaattcat
    16801 ttcgagctgt ctaatggata atcctttttc ttttgctaac tcctttatta tttcgtaagt
    16861 attcattgat aaatcaacct ttctgaatgc ttacaaaaaa agtttagata aataatgtaa
    16921 aaatagttga cttatataca ttattaatct atactatgtt ttgtaaacaa gtttaacaac
    16981 taaaaagaca acaaaaaata ctattgatta ataaatgcca accgccaaga aagcttttta
    17041 aatcaaagtt tatatgtctt atttaactat gcatttagta tagattatta atctaataaa
    17101 gtcaacgcag ttttttaaaa agttgttaaa tttgtttacg aatataaaag aaaggagaga
    17161 agaatatgga aaaaacagtc tcggcaaaaa tcgaagattt gaaattagac atactgaaac
    17221 aagcaaaagt ggcgatggaa cacgcggtag ataaagaaga ctctgccatg gttgcagcca
    17281 tagcagagat tttagctcac gtttagtcat cgtttttacg gtcttctgga agctgaccat
    17341 agataacagc ataatgaata tctaaataag cttgaacaat ttcttttggc gaaattgatt
    17401 cgcctttagt agatacttgt gattcgtgat ttagccatgc aacaactaaa tccgcagcga
    17461 tttcaaacgg cgttacactt tgtgtcagtt gccataaaga atttcacaat atgtatggtc
    17521 gtggtaataa taccaaagag caatttgagg aatttatgga aattaaaaaa tacaaaaaat
    17581 catttattaa aggaggctaa acaatgaaca aggaaatgag ccaaacaatc aaggtacaaa
    17641 aaatgataga tgatctaaca catggaattg atagccaagc cgataaaatc atcaaggaat
    17701 tgcaaggaca aaaagttaag gatgcaaaaa tgcttctcaa gactatcaac tttgaaatga
    17761 atccaacaaa aagaaagctc gccgatgtgt tggaagaaaa gttggcttcc gctatcaacg
    17821 agcaagaatt actatttgaa accgatactt ttaacagctg atgttttata gatgacagtt
    17881 tcatccgaat ctatatcatc gccgagagaa aagaatgttg cttttgacaa aaagccgata
    17941 agacctacaa gcggtcttga tgttgcaaga gtagataccc caatatcacc ttgcaaggta
    18001 ttcacgtaaa tttgagaaga atagaacatt ccatcttttt cttcgatatt tgacgcatca
    18061 tcgtatccaa cgaccaaagt atcggcaaat attttgattt tttctccatc aagcatagta
    18121 attacaaatt ctggtttcat aaattttcac ctccttaaca attatttcag cctgtcacac
    18181 tgataaggaa attataccaa agaaaggaat gagaaatatg aatacaccac aaatttttaa
    18241 tttcgagcaa aacgaagttc gaactatttt agtaaatgat gaaccatatt ttgtaggaaa
    18301 agatgtagca gacgttctag gatattcgaa tccgcaaaaa gctattcgag atcatgtgga
    18361 cttggaggat aagacgcaga acgattcgtt caccgtcaat ggaacagcag ttgttttgat
    18421 taacgagtca ggcctttaca gtttaatctt aaaatcaaaa cttccctctg ccaaaaaatt
    18481 taaacgttgg gtaacgagtg aagtccttcc aacaattaga aaaacaggta gctattcaaa
    18541 cgtacctcaa agttttgcac aagcattgcg tttagcagcg gatttagaag aaaagaacca
    18601 attactcgaa caacaaattg ccgagtacga accaaagatt agctacttag atacgattct
    18661 ttcatcgaca gatacggtag ctacttctca aattgcagct gattacggaa tgtcggcaat
    18721 tgctctaaac aaattgctaa acgagttagg tgttcagcat aaagttagcg gacaatggat
    18781 actttaccga aaacatatga accaaggata cacaaaatcg cacacaagtg agataccgaa
    18841 agccgatggc ggcactaaag ttgtaatgaa taccaaatgg acacagaaag ggcgagtgtt
    18901 tatttacaac ttattaatcg cagagggcta ttaccctcaa atggatttag aggaaattgg
    18961 ttagaaagga gttttagtat gactgacatt gcagaaatca ctcaacgaga tagagaaaaa
    19021 atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca ccatgcttgc tgaaagattt
    19081 ggaattagca aaagctactt atctttaatt ttaaacggta aaaagacttc tgcagaagca
    19141 aacagaatta tagattcgat tatcactatg tacgaattgt agagggaggt aaagtcgttg
    19201 gaagataaaa tcattcttac aaaatcagag cttcaagaaa tgttaaacat cgagtatgga
    19261 acgataattt ttaggaggga taataagtga aaaaaccaac actttcagag ctgatagaag
    19321 ctgctgagaa ggcagtaaaa ccagacgact ggtaccgaca aagtttaatc ttggagaagt
    19381 tccacggcat gtcaaaaact actttagttg aatactgcaa ggaaatggaa acaattcctg
    19441 aatttgcaga aggaatactt cgtccaggtc actcaaccac atttattcat taccatactt
    19501 ttatttggtt tttaaagtgg aaagacgcaa acaaatatcg tgtaaaaagt ttatcgcctt
    19561 cagatgtttt gaaggaggca agttaatggg caaattcaac agagcactag tattcagcgc
    19621 accgctaatc atctacgctt taggcctctg gggaagcaga caagcgttga tagggacaat
    19681 cgtttacatg gtttggattt ttatggggct tgatgaagct gagcacagag cgaaaaaagc
    19741 cagtcgggag ggactgacta aaatatgatt tttttatttt tgttcctttg ggctgctatt
    19801 gcgtacttca tccagctcct tgttgtcaaa gttgctctta tttcttttag gaagaacaaa
    19861 agaaaatagc gctataaatg aaagataagt agccgctaca ttaaactgtt cttttgaaaa
    19921 agttacagtc atcggaatga taattgatat tgaggaagta atcttaatta aaaatatagc
    19981 aatattttca acatcgcgtt ggtttttatt taacattgtc caacatgttt tagcaacaat
    20041 aaaaataatt acacatgata ttagcacaca gactgaaaaa agttttagaa atgcgatgta
    20101 gtcagtgtta ttgaactcct tcaaaatact atttttattc aatgtaataa aaggaatttt
    20161 tttaattgaa gacaggaaag ataaggtaat aaacagaatt atatatatgg ttaaagtcgc
    20221 agtaagaatg taattgagct ttttcctcat agaatcacct cactttctaa cgaaaattat
    20281 accaaaaagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt
    20341 aactacgagc ttacaagtgg cagaaagttt caaaaagaaa cataagcatg tactagaagc
    20401 aattgaatca ataaaaagat cggtcgaaaa ttcggccaat gttgaagatg ggtccaattt
    20461 tggacagatg tttgtggaag ggaacgagcc agactcatac ggaagaagtc ggagagttta
    20521 tttcatgaat agagacggat tttccttgct agctatggga ttcactggaa gtaaagcaat
    20581 aaatttcaaa ctaaaattta ttgaagcttt caacgaaatg gaagatgtta ttcggaagaa
    20641 tactgttcct caaacaattg aagacatgat gatctatcaa ttagaagaaa tgaaagatgt
    20701 taaaaaagat gtttccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga
    20761 aattaagcaa aaaggaaata tgaaagttat ggaagttcta gggggaaaag aaagccgagc
    20821 ttatgaagaa atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt
    20881 ttcaatccca agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat
    20941 tgaaatgtgg ttaccagaaa ctgcgatccg catggaaatc gatcaactga acagacaaca
    21001 aagacttttc ggtgatgaaa atgaatagag ctgaagcgct aagaataggg acggtaattg
    21061 ctaatcgctg gtggagacac aataaaccaa acatcctaag ccaacaacat atcgaaaagc
    21121 aaaaagcatg gcaacaaata aaaaagtgac tcagccggca agcatagagt cacaaaacat
    21181 atctaaggag aattttagca tatgaataaa gaactttcca ctttagatca atatttgact
    21241 gattctgaat ggggcaagtc gaatatcaag gaaacaaata atcgaaaaat cagacgaaat
    21301 cttttgacga acgaagaact atcatgtgat caagatgact tgggaaattt tgtgagtatt
    21361 tgggatcatg tttaccttat ccatctatca aaacattcaa ataaacctga atacatctat
    21421 gtcatcgaag atggcttgat tgatgcgcta gaagagtaca acagagataa cttgattgat
    21481 atctcttatt acggatcagg taagaaatac attgctgaaa tggaggcaga atttgatgag
    21541 tgaaatcaaa gggacaacga actttgaaaa actttttagt cgtaagttaa ataaaattct
    21601 caagaaaaaa ggaaattttg attatttatc ttgggctcac gcgtgggaga ttatgaaaaa
    21661 gaatgatcca caggcaacgg taactattaa tgagtataaa cactacaggg ttgtttctgg
    21721 aactcatcaa gactttcttg ttgaggaata taaacctttt cttatggatg aaacagggac
    21781 ttatgtatct gtctcagtaa cggttaaagg acatacggaa acaga
//
LOCUS       DAYYOA010000040        21750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000040 DAYYOA010000000
VERSION     DAYYOA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.040"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..1104)
                     /locus_tag="ACHBKA_001352"
     CDS             complement(205..1104)
                     /locus_tag="ACHBKA_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9445051.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="ACHBKA_001353"
     CDS             complement(1114..1470)
                     /locus_tag="ACHBKA_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9445052.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="ACHBKA_001354"
     CDS             1825..2121
                     /locus_tag="ACHBKA_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM9445053.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="ACHBKA_001355"
     CDS             complement(2765..3718)
                     /locus_tag="ACHBKA_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9445054.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="ACHBKA_001356"
     CDS             complement(3741..4496)
                     /locus_tag="ACHBKA_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9445055.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="ACHBKA_001357"
     CDS             complement(4493..5452)
                     /locus_tag="ACHBKA_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9445056.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="ACHBKA_001358"
     CDS             complement(5445..6395)
                     /locus_tag="ACHBKA_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9445057.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="ACHBKA_001359"
     CDS             6545..6997
                     /locus_tag="ACHBKA_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM9445058.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACHBKA_001360"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACHBKA_001360"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM9445059.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACHBKA_001361"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACHBKA_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM9445060.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="ACHBKA_001362"
     CDS             complement(10549..11358)
                     /locus_tag="ACHBKA_001362"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9445061.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACHBKA_001363"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACHBKA_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM9445062.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACHBKA_001364"
     CDS             complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACHBKA_001364"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM9445063.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="ACHBKA_001365"
     CDS             complement(16002..17783)
                     /locus_tag="ACHBKA_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9445064.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="ACHBKA_001366"
     CDS             complement(17809..18723)
                     /locus_tag="ACHBKA_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9445065.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACHBKA_001367"
     CDS             complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACHBKA_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9445066.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="ACHBKA_001368"
     CDS             complement(19712..20656)
                     /locus_tag="ACHBKA_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9445067.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="ACHBKA_001369"
     CDS             complement(20657..21661)
                     /locus_tag="ACHBKA_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9445068.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6079 a         4640 c         3849 g         7182 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat
//
LOCUS       DAYYOA010000041        21560 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000041 DAYYOA010000000
VERSION     DAYYOA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.041"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..1430
                     /locus_tag="ACHBKA_001370"
     CDS             261..1430
                     /locus_tag="ACHBKA_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9445069.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1903..2457
                     /locus_tag="ACHBKA_001371"
     CDS             1903..2457
                     /locus_tag="ACHBKA_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9445070.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2630..3067)
                     /locus_tag="ACHBKA_001372"
     CDS             complement(2630..3067)
                     /locus_tag="ACHBKA_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9445071.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3115..4062
                     /locus_tag="ACHBKA_001373"
                     /pseudo
     CDS             3115..4062
                     /locus_tag="ACHBKA_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4333..4768
                     /locus_tag="ACHBKA_001374"
                     /pseudo
     CDS             4333..4768
                     /locus_tag="ACHBKA_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4784..5617
                     /locus_tag="ACHBKA_001375"
     CDS             4784..5617
                     /locus_tag="ACHBKA_001375"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9445072.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5862..6887
                     /locus_tag="ACHBKA_001376"
     CDS             5862..6887
                     /locus_tag="ACHBKA_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9445073.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7025..7150
                     /locus_tag="ACHBKA_001377"
     CDS             7025..7150
                     /locus_tag="ACHBKA_001377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445074.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7175..8290
                     /locus_tag="ACHBKA_001378"
                     /pseudo
     CDS             7175..8290
                     /locus_tag="ACHBKA_001378"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8373..9269
                     /locus_tag="ACHBKA_001379"
                     /pseudo
     CDS             8373..9269
                     /locus_tag="ACHBKA_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9285..10109
                     /locus_tag="ACHBKA_001380"
     CDS             9285..10109
                     /locus_tag="ACHBKA_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445075.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10141..11451
                     /locus_tag="ACHBKA_001381"
     CDS             10141..11451
                     /locus_tag="ACHBKA_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM9445076.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11599..12858
                     /locus_tag="ACHBKA_001382"
     CDS             11599..12858
                     /locus_tag="ACHBKA_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9445077.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12868..13740
                     /locus_tag="ACHBKA_001383"
     CDS             12868..13740
                     /locus_tag="ACHBKA_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445078.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13752..14582
                     /locus_tag="ACHBKA_001384"
     CDS             13752..14582
                     /locus_tag="ACHBKA_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445079.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14622..16805
                     /locus_tag="ACHBKA_001385"
     CDS             14622..16805
                     /locus_tag="ACHBKA_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9445080.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16866..18086
                     /locus_tag="ACHBKA_001386"
     CDS             16866..18086
                     /locus_tag="ACHBKA_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9445081.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18083..19543
                     /gene="gtfA"
                     /locus_tag="ACHBKA_001387"
     CDS             18083..19543
                     /gene="gtfA"
                     /locus_tag="ACHBKA_001387"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM9445082.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19620..20726
                     /locus_tag="ACHBKA_001388"
                     /pseudo
     CDS             19620..20726
                     /locus_tag="ACHBKA_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21048..21257
                     /locus_tag="ACHBKA_001389"
     CDS             21048..21257
                     /locus_tag="ACHBKA_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9445083.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21250..21399
                     /locus_tag="ACHBKA_001390"
     CDS             21250..21399
                     /locus_tag="ACHBKA_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM9445084.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT         7415 a         3223 c         4130 g         6792 t
ORIGIN      
        1 atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc aaagaaagtt
       61 taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta
      121 gtgatataac gaaatatcta cacagacaaa atgatagcgt aaaatatttt gtgtaaatag
      181 atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa
      241 ttttatagga ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca
      301 ataaaggtga tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat
      361 tgcaggctga attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt
      421 caggtaattc ccgtaatggg aattattcac gttcattcag aacagagtat ggagaattaa
      481 atttggtaat tcctagagat agaaatggta agttttctca acaaacttta cctgcttata
      541 aaagaaccaa tgactcttta gaaaccacta ttattcagct atttcaaaaa ggaattacta
      601 tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca tcactatacg ccacaaacca
      661 tttcaaatat gactaaaata gtttctgaag atattattgc ctttaaagaa aggtccttag
      721 aatccagata ctctgtcatt tttatggatg ctactcacat tcctttaaaa agacaaactg
      781 tttcaaaaga agcagtttat attgtaattg gcattcgatt agatggaacc aaagaggttc
      841 ttgggtttag tcttgctcca actgaatctg cttatgtttg gaaagagatt cttcaagacc
      901 taaaagaccg tggtttaaaa gaggttttat tagtcgtaac tgatggttta agtggcatta
      961 acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat
     1021 ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
     1081 aattagttta ccaagcaagt tcaaaagaag aagcaaataa tcaaataagg tttatgatag
     1141 ataaatggaa aaagcagtat ccacgagtgg ttaaattgct catgaatcct gctatattaa
     1201 ccttttataa tttccctccg tcaatcagaa gaactattta ttcaactaat ttgattgaag
     1261 gttttaacaa acaattaaag aaatatacca aaagaaaaga gcaatttcct aatgaagaat
     1321 ctctggagag atttctagta tctcaattca acaactataa tcaaaagttt ctatgccgag
     1381 ttcataaagg ttttaaagaa atacatgata cattagaatc aatgttttaa cttgatcaac
     1441 agaatgggct tttccattta cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc
     1501 gtaaagttat aaaatattaa taaaaatatt ttattaatga aaagcttggt agcacggtta
     1561 ttcatatgct aatttatttc actttatagt aaggggagtt cttagtatat tgttcaaact
     1621 taaatttcat ataatttaat tatgtaaatt agctattcta agaacttcta cggataactg
     1681 cccgtctaaa atttaaataa gagcttagaa tcttcatata agtaaggtta atttttttgt
     1741 acaaaaaagt cggaatattc tgagttactg aaatgatata aaatcataaa ctaaatccct
     1801 tgattttatt gactttcaaa agtttatata tataagttaa tgattatggt aaaatatata
     1861 cataatcatt aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa
     1921 ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat
     1981 ccggaaagag atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat
     2041 attataggat taaaagttgg agatatacga ggcaaaagta agcctattat tgttgaacat
     2101 aaaacaggta agcgaaaacc gatttttata gataatttga gagaagaaat tttattatat
     2161 actgaaggta aagaagaaaa tgattggtta tttccgagtc ggcaacaagg tcgacatata
     2221 acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg acgagatgat
     2281 ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa aactagagac
     2341 attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt
     2401 ggtataactg aagatgaaat aggagcatca cttcgtggat ttaaactagg tgtataattt
     2461 aaaaattttt ataaacagta aagacaaaga ttaaaagcta tttttatcta gaacgatttt
     2521 atttcataaa gttcgcggaa taaaagctgg ggttactggt acttggtata ggttcgtgtt
     2581 atataaacta agtaattaaa taacattttt aggttggaaa aggatgaaat taaaaaaaga
     2641 gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa gaaaaatact gataactcaa
     2701 cgtttagcag actatctaat agggatagtc tgcttttttc gtttttgacc atacttttga
     2761 ccttctgcaa acttccttgc ttttcctctg tagaactcat gaaatctgca aaacgaattg
     2821 ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat atcatccata gtggttttaa
     2881 tatctgtatg tccaagtcga acatacactt ctttgacaga cgcaccagat tcaaagagca
     2941 gactacaatg ggtatgacga aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa
     3001 tgatgatttt caaattgtga tttacatagt ctaaataaag gggcttattt tcttctgatg
     3061 aaaacaaaag ttgttcacca tcttttatag gtttgattct catttttaga tattatgccc
     3121 gcacgtattc atcacatttc tattgtgaat cgctttattc gaccgacatt tgacttttat
     3181 cataatatac tcggtttaaa attactaatg aaaacaatta atcaagacga tcataccatg
     3241 tatcacctgt ttttttcaga taatcaccac cgtgtgggaa ctgaactgac tttttttgaa
     3301 gtacaagaag ggaacaacca gttttttgga accaatacga ttgaacgaac gatactaaaa
     3361 gttccttccg aagcatcatt gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc
     3421 cattatggaa tcgaatcatt ttctggacga cctatcttac gttttgaagg gccagatgca
     3481 acacgattag ctttagttcc cttacgcgaa tttgaagata ttgatgatta tttcccgtat
     3541 gtcacagacc agatccctac agagcatgcc attttaggaa ttgatgccat tcaactacgc
     3601 gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg taggctggta gcacaaagaa
     3661 tacgtcagtt tcttttcggg tcatcaacga gtgacaatct tagaaaatca agacactcag
     3721 ttctatcaag aagttcagat tattcaagat cgtgagaatc cagttgccat tgaagggatt
     3781 ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat ctgatttaca acgcatcgac
     3841 aaacaattac aagagcgcaa ttttataaat tcaggaataa aagatcgtga attttttgta
     3901 tcactttatt atcgtgaccc aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac
     3961 ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg aagaaattcc tttattttta
     4021 ccgcagtact taaatttcat tcgtgaacaa gtggagcaat aattagctaa tcaaatccat
     4081 gacgaagagt aagaggttga ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat
     4141 tctaaagaat gttgatttga tgcggttcct atttttataa actatcattt tcgactaata
     4201 aaactactta tgagtcagcc tctctttttt tcttgatttt tatacctctt tctaggtaaa
     4261 ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa
     4321 ggggagtacc aaatgaacga gtatattgaa attatccacg caagagaaac aacttaaaaa
     4381 tgtcagtctc aaaattccca aaaataaaat tagtattttt accagcgtat ctggttcagg
     4441 aaaatcatcg attgtttttg atactattac ccaagaagct ggtcgtcaac tgaatgacac
     4501 ctatgatagt tttacccgtt tatttttacc taaatacagt cgtccagagg ttgatgagat
     4561 tgataatctt tctacggcga ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc
     4621 aacgttaggg acgattacta acatcaatgc gttatttcgt gtgttgtttt catgttttgc
     4681 aacacctagt ttaggatatg caaatgctta ttcatttaat gatcctagtg gaatgtgttc
     4741 tgtctgtcag ggaatcggta agacgattgc atttttaata ggattgcttt atttttcggc
     4801 tattgataat gctgattttg tattttctga tccatttacc gcagaagttt tgtctggaat
     4861 tgaggaacag gcaaagaatc atggttattt tgtattggtg cataatgtta attcagcaga
     4921 agatgttaaa actattcaga gaaattggcg ttttgagggg ttcattgttg ttggtgtacg
     4981 tgtaaaagaa tttttggaat taaataacgt ttttgatggt cctgttgttt atattgatac
     5041 gcatctttca aaagagacat taagagaact tgaacaacaa atgaatcgtt tatttgtaaa
     5101 tacaaatgat taccaagcta gtacattggc agtggaacat ttgattcatt atggtcatga
     5161 gaaaatagcg tttctttctt ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg
     5221 acatcaagct tatatcgata cattaaagaa atatcattta acctttaaat cgcattggat
     5281 atataacaac tctgaatttg agcgaattta tcaagaatta gctgaattta ctgcaattgt
     5341 tgttactgga gattatttag cggctaaatt agtaaaattt ttaaaaaata agaataaacg
     5401 agtgatggaa caattatcga ttattggatt tgatgatatt acttttgcag aattaatgga
     5461 tcctgcgttg acaacaatac gactaaatcc aggtaacaag ggggcgattg cttttcacca
     5521 actgattgga ctggccaaac aagaagaaat aaaaaatcaa gtactactat tagagggaga
     5581 attgatttct cgagactctg ttcacaaaat caaataaaaa agctctgtga aataagtata
     5641 gttaacaata aaatatccga acgatagaga gaatgaactg tgatctagca gttcctcgtc
     5701 gcaaattcac tagaattagc attatttact ctaaattgtt cggatatttt ttcttgtttt
     5761 taattatgat tacagtgcct atttttaacg aaatttgctt attttcaaaa tgtatgatta
     5821 tatgttagta aacactttca aatatgaaaa gaggagctat tatggtagga attagggatg
     5881 ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg cgtgctaaat gaggatccga
     5941 cactttcagt aaccgatgaa accaaacgaa agattgcaga atcggttaat ttttatcatt
     6001 atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat tgcattaata acgacagttt
     6061 ctgaagtaga tgagttggaa gatccctatt ttcgaacgat tcgcagaggg attcaagtcg
     6121 aagcagaaca aagcaaagtc aatttgaaga aaattattcg tttgtcagaa acaccgttgg
     6181 agcaagacga tttgctaaaa tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg
     6241 tgattgaaga agtatcagca attaattcga atgtcgtagt gattgatgac cctagtattg
     6301 aagctgaagt agatgcagtt tatactgatt taacaaaagc aacgaatcag catttagatc
     6361 gattgtataa aaaaggacac cgaaacattg cgtttatcgg aggaggacgt gttcgtttag
     6421 atagccaagg aaataaacac gtgtcagatg atgattatcg aaaaatagcc tatgagacgt
     6481 ggatgaaaca aaaaggatta gaaaaatata gtcaaattta tttagaaggc tggaccacac
     6541 ttgacggaat gcaggcagca gatttacttt tgaaaaaaca tgctaaagat aagttgccga
     6601 ctgctatcat ggtaggaaat gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa
     6661 atggtctaca tattccagaa gatatttcga ttgtgagttt tgataatatt gaagttgctg
     6721 aatttttaac acctgcatta aatacggtga atgtgaatac agaagaaatt ggaaaactag
     6781 cagttcgcat ggttaatgaa cgaataaaag aagttagaaa tgtttcgatc aaagtgattg
     6841 tttctaataa tattattgtt agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc
     6901 caaaaaaggg tgtttacttt tttgtttact aatattttta gtaaaaaatc tcgacaaaac
     6961 attaagtaaa cagttatggt gttgttaaca aaaagaaaac gcattcaaaa caggaggaat
     7021 taagatgaaa catttcaaaa aaatagcttt aacagggata gctgttttaa ctgggacatt
     7081 actcgcagca tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa ctttaagcta
     7141 tgcgagttga gatactgacc aagctccagg actaagaaaa atgttagatg aatttgaaaa
     7201 acaaaatcct gatatcaaag tagaaatgga gacaacgcct tgggatcaat attgggttaa
     7261 attagaagct gcaacaacgg gaggtaatat gccagatgta gtaaccatgc actctagcga
     7321 atcctataaa tatatgtcac agaatgcttt gatgggactt gatgatgtta tcaaagaaaa
     7381 tgatattgac atgggaaact ttactgaagg aattgctgat ttctatacgc ttgaagacga
     7441 actatacgca atccctaaag atgcttctgt agtggggcta tggtacaaca aagaattatt
     7501 tgataatgca ggtgtcgatt atccagatga aacatggaca tgggaaacac tcagacaagc
     7561 tgcaattgat ttaacagatg aatcaaaagg catctatggt tttgcatcac atattggaac
     7621 agagactggc tactggccat ttatttatca aaatggggga gaagtatttg ccgatgacaa
     7681 tacaaaatca ggtttgaata ccccagaagc acaagaagct ttagcttttt atcgagatat
     7741 tgcacttgtt gatcatgctt caccaacaat tacagaatta caagatcctg ataagttaag
     7801 tcgttttcaa tcaggtagat tagcaatgat gattgaagga aactggtttg caccgggatt
     7861 tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt
     7921 taatgcaaca attacaaatg gacttggttg ggctgccagt gcgagtacaa aatacccaga
     7981 agaagtgaaa aaactaatgg catttttagc tagtgaggac gccaataaaa tccaagcgga
     8041 aacgggggca tcaatcccag cactaaaagg ctttgaagac gagtgggctg cttcttcaga
     8101 tacaattgat ttgagtgctt ttggaaaaat gttggagtat ggagtccctc gtccatacaa
     8161 taaagatgga ttacgagctg aacaagcaga agttcaaatt attaaccgta ttctgtctgg
     8221 tgaagtaact gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg cgatattgag
     8281 cggaaattaa attagttaat tatacaatat acaggcgaaa ggattctatt tcgtctgtat
     8341 attaatggat gaataccaaa aagggggata ttatgaaaaa tcgaattttt ggaaaatatc
     8401 gtaaagatca agtgatatgg ggctatattt ttatttttcc aacggcatta ggattaattt
     8461 tattaaatat atggccagca attcaaacag caattttaag ttttcaaaaa acggtagggt
     8521 tcggtgatac tgaatgggtc ggattaagaa attaccaaaa aatgtttgca gacggtgaag
     8581 tttttcagtc tttattaaac acattaattt atgcagtggt ttcgatacct agtattgttg
     8641 tattatcctt gttggtggca gttttaatga atagaaaagt taaaggattg tcgatttatc
     8701 ggacaattta ttttctacca gtcgttgctg caccatccgc agtcgcaatg atttggcgct
     8761 ggatgttcaa taatgattat ggactgatta ataacatgtt ggctaaaata ggtctcgatg
     8821 gaccagcatg gttaacggat ccatcgattg cgatttattc tattatcatt gtaggagttt
     8881 ggagtgctat tggttataac atggtgttat tactagccgg cttataagaa attcctaaag
     8941 attattatga agcagcggaa attgatggtg ctagtccaat ccagcagttt ttcaacatta
     9001 cgttaccatt agttacacca acattatttt ttgtagttgt aacaacagtt atcaatgctt
     9061 tccaagtatt tgatgttatt tttatgatga ttactcctaa tagtaccgcc atgtttaaat
     9121 cacaatcatt agcctattta ttctataaac attcatttat tttaaatgac aagggatatg
     9181 gttcggcaat cgttatgttc cttttagtga ttatattaat tattactgca attcaaatga
     9241 agttgcagaa aaagtgggta aactattaag gaggctatca aattatgaaa aataaatttt
     9301 tttcaaatgc gccaatccat atcatgctta ttttaggtgg tattgcaatg gcattacctt
     9361 ttatttggat gattttaact tcagggaaaa cgttggttga atctacccaa attccaccta
     9421 caattttccc tgagaaattt cagctaagta actatcaaca agtacttgat ttattaccat
     9481 tcatggcttt ttatatcaat acatttttaa tggttgttgg acgagtgatt ggctcggttt
     9541 tattttcagc aatggctgca tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc
     9601 ttttcagctt agtacttatt caaatgatga ttcccggttc catttttatt actccacagt
     9661 atttattagc acaaaaattg ggtattttaa atacaattcc agcattaata ttaccagggg
     9721 tagttagtgc gtttggtacg tttttattaa gacaattttt cttaggttta cctgatgaat
     9781 tggaggaagc ggcctattta gaaggtgcca atatttggac gatctttttc aaaattatgt
     9841 tgccgcttgc taaatcagga ttggttgcac ttggaatatt cacagcgttg tttgcattta
     9901 aagatttaat gtggccattg attgtcaata cttcaattga taagatgcct ttagctccgg
     9961 gattagcaaa cttacaagga caatatgcca cgaacttccc acaattaatg gcaggttcgt
    10021 tattagcaat ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag
    10081 gaattgctac aacaggtagt aaattataaa actattaatt agtgattgga gagtaagtac
    10141 atgacaaaaa ttacatttat tggtgcagga agtactattt ttgcaaaaaa cgtattaggc
    10201 gatgcgatgt taactccaag tctacaagat gcacatattg cattgtacga tattgatgaa
    10261 aaacgactaa aagaatcaaa tttaatgctt aaaaatatta ataaaaattc gaatgaaaat
    10321 cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt taagagaggc tgattttgtt
    10381 atcaatgcta ttcaagtggg tggttataaa ccttcaacag tgattgattt tgatattcct
    10441 aaaaaatatg ggttgcgaca aactattggt gacaccatgg ggattggtgg gatttttaga
    10501 accctacgga cactaccagt aatgttagac tttgctaaag aaatggaaga agtttgtcca
    10561 aatgcttggt tattgaacta tacaaatccg atggcgatgt taaccatggg tgttttaaaa
    10621 gcgacgagta ttaagactgt tggtttatgt catagcgttc aagtatgcgt accagaattg
    10681 tttgagcatt taggaatagc cgatcaatat gacttaaatg actttcaatg gaaaattgca
    10741 ggcattaatc atatggcatt cttgttggaa attacaaaaa atggggaaga tttctatcca
    10801 acaattcgtc agttagctgc agaaaaacca aatccacatc cagattctgt acgttttgaa
    10861 ttgttgaaac gttttggtta ctatattaca gaatcaagtg aacacaatgc agaataccat
    10921 ccatacttta ttaaatcaaa atacccagaa ttgattgacg aattagggat tccgttggat
    10981 gaatacttac gtcgttgtga acaacaaatt aatgattggg aaagcatgcg agaggacatt
    11041 gtaaataatg gggagttaac acatacgaga agccgtgaat atgcttcata tatcatggat
    11101 tctattacga caggaactcc gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc
    11161 acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg
    11221 caaccaacct atgtcggtga attgccaaca caattagctg ctttaaatcg gacgaatatc
    11281 aatgttcaag aactgacagt agaagccgct ttaacactag ataaatcaaa aatttatcaa
    11341 gcagcgtatt tagatccaca tctatcatct gaattgtctt taacagatat caaaaattta
    11401 gtggatgatt taatcgaagc acatggggac tatttacctg aatataatta aaaagaagct
    11461 gaaagagtaa ggaattttca attccttact cttttcttag ttaaatttag taaaaatatt
    11521 gacaaaacac ttagtaaaca gttacattag tgttaacaaa aagaaaacgc attcaaattg
    11581 tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg tatagcacta
    11641 ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta
    11701 gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga gattgctaaa
    11761 gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc gaatgctggt
    11821 gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa tctttatcct
    11881 caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt aaccgaagaa
    11941 ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga ggatcgtgta
    12001 tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac tgcttttgag
    12061 gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt agctgagatt
    12121 aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac tttaaatggt
    12181 tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa caatgtttgg
    12241 cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa agattttaat
    12301 cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg tgccggttat
    12361 aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg
    12421 gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt tccttttcca
    12481 gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt atcagtttct
    12541 gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac aacacctgaa
    12601 gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag
    12661 aatactcagg attcaccatt aagtggttta actgaacttg cttttactga tcgtcattta
    12721 gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac aacaaattat
    12781 ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt taatccaatg
    12841 aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa tcgttactgg
    12901 gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta ctttccaatg
    12961 gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa ttttgatttt
    13021 gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa atcgattggc
    13081 ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg tattttgatt
    13141 gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg gttctttttt
    13201 ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt taactatggt
    13261 ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct tttaggaaca
    13321 acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat tgccatgcca
    13381 gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga agctgctaga
    13441 atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta cttattacca
    13501 agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt tgatcaaatt
    13561 tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt acttgtttat
    13621 aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt agtcttattt
    13681 atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt tgaaatctag
    13741 gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg cttttattat
    13801 tagggggctt catgatttta gttcccttag tttcaacgct attcggttca tttaaaacaa
    13861 cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc
    13921 gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc atcacaatca
    13981 ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta gcaagaaaaa
    14041 tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga atttttgttc
    14101 ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt ttagcaaata
    14161 tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta tttttatatg
    14221 ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa attgatgggg
    14281 ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca atgcatgcga
    14341 cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca ttattaattt
    14401 taaatcgtga ttctaatatg tggacactac cattgtttca atataattat acgggacaat
    14461 attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc acgattacac
    14521 tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga gcagtgaaat
    14581 aaccaataaa ttattctcat tataaaggag ttcataaact tatgattata tttgacaatc
    14641 acacacaaac atttcattta caaaataaaa aaattagcta tctattagga atcgaagaaa
    14701 ataattattt aacgcatctt tattttggaa aaaagattac gcactattct ggtgggtatc
    14761 gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat
    14821 tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat tttagagaac
    14881 cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct
    14941 acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat acggaagatg
    15001 atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga ttgactttac
    15061 agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg caattagtaa
    15121 ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt gattttccaa
    15181 atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt caaatgacta
    15241 gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca agcagccatc
    15301 aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat
    15361 atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa gatccatttg
    15421 accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa ctaaaaactg
    15481 gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt ctgaatacgt
    15541 tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac tatcgtttaa
    15601 aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt aacgaagaaa
    15661 aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt gttttagatg
    15721 atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg gttgaaacag
    15781 aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat aacgggatga
    15841 aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta tttcgagcac
    15901 atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga aatcaatatg
    15961 ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg acggcgattt
    16021 ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg acagaagtct
    16081 attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat atgttagggt
    16141 tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt gaaagttgtt
    16201 cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa acttggacga
    16261 gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc ttagtgtatc
    16321 caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa
    16381 cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat gagctagatc
    16441 taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc ttttataaaa
    16501 agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg tttgaaggca
    16561 atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc ttttatttcc
    16621 gagtattagc agaagctgcg catccattag tcacattgaa attagcagga ttagcagaag
    16681 aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg aatctcggtt
    16741 tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat ttgaaacaga
    16801 tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt ccaaacgaag
    16861 aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat tgggaaaatg
    16921 aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa ggactaggcg
    16981 gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc atctttgaat
    17041 ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag tcgggcattg
    17101 aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt atgacggtag
    17161 cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat ggctattatt
    17221 cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt gaatggttta
    17281 aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat caattagtta
    17341 cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca cgttcaatta
    17401 ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg gcgacgcttt
    17461 ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga atgaccaaca
    17521 tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag tataaacggg
    17581 cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg
    17641 ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc tcaacttctg
    17701 ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa gctcagttaa
    17761 ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga aaaaatagtg
    17821 tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa aatgaacata
    17881 ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta aacgttacag
    17941 caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc aataattatg
    18001 aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa agtattgtat
    18061 tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc
    18121 agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac atttacaagg
    18181 tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc gaggctttgc
    18241 gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg tggaagcatt
    18301 aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc gtgaatcaaa
    18361 atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa tgtttatccg
    18421 tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt taatctacaa
    18481 gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga cagaagaggt
    18541 ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag ttgtgaaaga
    18601 atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa ttcgattaga
    18661 tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg aaccagatat
    18721 ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc
    18781 agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt attatatcta
    18841 tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat cagaacgttt
    18901 agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata cgcatgatgg
    18961 aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt acacctctga
    19021 ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca attacaataa
    19081 tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata atgaccgtag
    19141 ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa tttattacgt
    19201 tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag aagggcgtaa
    19261 tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac ggccagtgat
    19321 tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg atttggatgg
    19381 aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta aaaataaaaa
    19441 cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta tcattgagtc
    19501 taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc catagaacaa
    19561 aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag gactcataca
    19621 tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata aaattaggaa
    19681 aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt
    19741 cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc
    19801 gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat gtgtcatcga
    19861 ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg ttagaagaaa
    19921 tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag cgaaatcatc
    19981 tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag tgggcgactt
    20041 tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg gacgatacta
    20101 cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc aatcaactac
    20161 cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat taggactttg
    20221 aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt aaaaaaaaca
    20281 ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt ctacgagacg
    20341 agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta gtcactgttc
    20401 aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc tcttcaaaag
    20461 aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag cacatcaaac
    20521 aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga
    20581 ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg aaaacggaaa
    20641 aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc tttgaatgtt
    20701 gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac ttttggtttt
    20761 cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc agcacagttt
    20821 tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca
    20881 ctagtgatat tttttagaat tagatttcta atgtgcattt tttgagtatt aaggagcaag
    20941 tatacaaaga tttataaagc tgattaaatt tgaataaaaa tatgcaggaa cgctactatt
    21001 tttagtagtg ttctttttta tatttgaata aaaaatgtgg tggttaaatg gtggttataa
    21061 caaaaaatga attaatgaaa ttaggttatg ggaagtatca gtctgagaga atcatacgac
    21121 aggcaaaagc aattatggtt tcaaaaagct atacctatta cgaaaataga agattaggac
    21181 tagtacctgt tgaatctgta gaagaagttt taggtatgaa gatagaggta gaggaggatt
    21241 ttcagaatca tggttaattt taaaaaagat aaaaatggga aatatcattt tactgctagt
    21301 ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag
    21361 ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat tatggaaaag aagcagaaac
    21421 ttctcaaagt actatgttgt ttgaggagtt tttttataaa ttattcctac cttattatca
    21481 agggaaagta gaagaaagaa catataaaaa tcggttatct acaatcaaaa aaacttttct
    21541 tatttcttta aaatggagtt
//
LOCUS       DAYYOA010000042        20484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000042 DAYYOA010000000
VERSION     DAYYOA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.042"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="ACHBKA_001391"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="ACHBKA_001391"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM9445085.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="ACHBKA_001392"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="ACHBKA_001392"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM9445086.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="ACHBKA_001393"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="ACHBKA_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM9445087.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="ACHBKA_001394"
     CDS             2856..3731
                     /locus_tag="ACHBKA_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM9445088.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="ACHBKA_001395"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="ACHBKA_001395"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM9445089.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="ACHBKA_001396"
     CDS             4628..5062
                     /locus_tag="ACHBKA_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM9445090.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="ACHBKA_001397"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="ACHBKA_001397"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM9445091.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="ACHBKA_001398"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="ACHBKA_001398"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM9445092.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="ACHBKA_001399"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="ACHBKA_001399"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM9445093.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="ACHBKA_001400"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="ACHBKA_001400"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM9445094.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="ACHBKA_001401"
     CDS             13333..14259
                     /locus_tag="ACHBKA_001401"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM9445095.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="ACHBKA_001402"
     CDS             14441..15334
                     /locus_tag="ACHBKA_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM9445096.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="ACHBKA_001403"
     CDS             15468..15593
                     /locus_tag="ACHBKA_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9445097.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="ACHBKA_001404"
     CDS             complement(15727..16359)
                     /locus_tag="ACHBKA_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445098.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="ACHBKA_001405"
     CDS             complement(16507..17049)
                     /locus_tag="ACHBKA_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM9445099.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="ACHBKA_001406"
     CDS             17184..17573
                     /locus_tag="ACHBKA_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445100.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="ACHBKA_001407"
     CDS             17577..18341
                     /locus_tag="ACHBKA_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9445101.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="ACHBKA_001408"
     CDS             18470..19417
                     /locus_tag="ACHBKA_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9445102.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="ACHBKA_001409"
     CDS             19547..20287
                     /locus_tag="ACHBKA_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445103.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7192 a         3481 c         4316 g         5495 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
    20341 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
    20401 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
    20461 cgcagatcgt ttgcaaaaat agtt
//
LOCUS       DAYYOA010000043        19480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000043 DAYYOA010000000
VERSION     DAYYOA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.043"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHBKA_001410"
     CDS             133..3660
                     /locus_tag="ACHBKA_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9445104.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHBKA_001411"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHBKA_001411"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM9445105.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHBKA_001412"
     CDS             7448..7789
                     /locus_tag="ACHBKA_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9445106.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHBKA_001413"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHBKA_001413"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM9445107.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHBKA_001414"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHBKA_001414"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM9445108.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHBKA_001415"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHBKA_001415"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM9445109.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACHBKA_001416"
     CDS             13000..13737
                     /locus_tag="ACHBKA_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9445110.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACHBKA_001417"
     CDS             13751..14566
                     /locus_tag="ACHBKA_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9445111.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACHBKA_001418"
     CDS             14579..15226
                     /locus_tag="ACHBKA_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9445112.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACHBKA_001419"
     CDS             15242..15898
                     /locus_tag="ACHBKA_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9445113.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACHBKA_001420"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACHBKA_001420"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM9445114.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACHBKA_001421"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACHBKA_001421"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM9445115.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACHBKA_001422"
     CDS             18240..18758
                     /locus_tag="ACHBKA_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9445116.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHBKA_001423"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHBKA_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM9445117.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3344 c         4182 g         5402 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DAYYOA010000044        19221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000044 DAYYOA010000000
VERSION     DAYYOA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.044"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..849)
                     /locus_tag="ACHBKA_001424"
     CDS             complement(25..849)
                     /locus_tag="ACHBKA_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM9445118.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(822..1502)
                     /locus_tag="ACHBKA_001425"
     CDS             complement(822..1502)
                     /locus_tag="ACHBKA_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9445119.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1525..2463)
                     /locus_tag="ACHBKA_001426"
     CDS             complement(1525..2463)
                     /locus_tag="ACHBKA_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9445120.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2708..3559)
                     /gene="ispE"
                     /locus_tag="ACHBKA_001427"
     CDS             complement(2708..3559)
                     /gene="ispE"
                     /locus_tag="ACHBKA_001427"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM9445121.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3713..6286)
                     /gene="mprF"
                     /locus_tag="ACHBKA_001428"
     CDS             complement(3713..6286)
                     /gene="mprF"
                     /locus_tag="ACHBKA_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9445122.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6427..7233
                     /gene="proC"
                     /locus_tag="ACHBKA_001429"
     CDS             6427..7233
                     /gene="proC"
                     /locus_tag="ACHBKA_001429"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM9445123.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7312..8868)
                     /gene="rny"
                     /locus_tag="ACHBKA_001430"
     CDS             complement(7312..8868)
                     /gene="rny"
                     /locus_tag="ACHBKA_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM9445124.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9279..10328)
                     /gene="recA"
                     /locus_tag="ACHBKA_001431"
     CDS             complement(9279..10328)
                     /gene="recA"
                     /locus_tag="ACHBKA_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM9445125.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10437..11678)
                     /locus_tag="ACHBKA_001432"
     CDS             complement(10437..11678)
                     /locus_tag="ACHBKA_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM9445126.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11918..12496)
                     /gene="pgsA"
                     /locus_tag="ACHBKA_001433"
     CDS             complement(11918..12496)
                     /gene="pgsA"
                     /locus_tag="ACHBKA_001433"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM9445127.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12705..13628)
                     /locus_tag="ACHBKA_001434"
     CDS             complement(12705..13628)
                     /locus_tag="ACHBKA_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM9445128.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14367..15653)
                     /locus_tag="ACHBKA_001435"
     CDS             complement(14367..15653)
                     /locus_tag="ACHBKA_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9445129.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15646..16914)
                     /locus_tag="ACHBKA_001436"
     CDS             complement(15646..16914)
                     /locus_tag="ACHBKA_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9445130.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17166..17627
                     /gene="mscL"
                     /locus_tag="ACHBKA_001437"
     CDS             17166..17627
                     /gene="mscL"
                     /locus_tag="ACHBKA_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM9445131.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17687..18133)
                     /locus_tag="ACHBKA_001438"
     CDS             complement(17687..18133)
                     /locus_tag="ACHBKA_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445132.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18226..18471)
                     /locus_tag="ACHBKA_001439"
     CDS             complement(18226..18471)
                     /locus_tag="ACHBKA_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM9445133.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18586..19164)
                     /locus_tag="ACHBKA_001440"
     CDS             complement(18586..19164)
                     /locus_tag="ACHBKA_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9445134.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT         5503 a         4181 c         3332 g         6205 t
ORIGIN      
        1 tataaggctt cttttcttta taatttagtt attttttctt ctgaaggcaa ccaatattcg
       61 tttgatgata tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg
      121 aggtgtatct agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca
      181 aacactgata gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg
      241 aagaaccatg attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc
      301 cacaccagta atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg
      361 ggcggtatct tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat
      421 accgatggca acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat
      481 cgaaccaaac agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa
      541 tactaacgct agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt
      601 tcggtagatc gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt
      661 cgtaagtgta ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg
      721 agaaagcgta tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg
      781 ggcgattccg gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag
      841 caacgccatt gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg
      901 atatcttcat gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt
      961 cggagaagac gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca
     1021 agaacaaata agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt
     1081 ccgccagaca attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa
     1141 gcacgctgta catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga
     1201 tatcttcctg aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca
     1261 acttgttgag gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc
     1321 tttatcattc cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg
     1381 gctgctccat tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca
     1441 tcttctagta caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc
     1501 accttacaac ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt
     1561 ttccatgaca gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa
     1621 aggattcaac acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga
     1681 agaggcattt tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag
     1741 ctcagctaag cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg
     1801 ttttaagtcg tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg
     1861 gtttgttgca tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat
     1921 ataagcatcg gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc
     1981 ttcagcaatc gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc
     2041 gtattcatgt gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga
     2101 ttctaaacgg tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga
     2161 atcctttaac gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc
     2221 atcggaaatt tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc
     2281 agggatcaaa agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat
     2341 cggataaaaa gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt
     2401 tccacatgct gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt
     2461 catttatatg ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa
     2521 tgtatcaaaa taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta
     2581 tattcttgat taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca
     2641 ataaaaaact gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc
     2701 ttatatttta ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat
     2761 tatatactct ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc
     2821 cactcatcaa tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga
     2881 ctggatgccg ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct
     2941 gataatcttg ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt
     3001 gcgtatctac ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca
     3061 caacccagca ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa
     3121 atgccgtact tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg
     3181 ccagttcttc taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg
     3241 cacagtctgt acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga
     3301 tcttgacacc ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat
     3361 tgttccgttc atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg
     3421 ttatttcttc aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact
     3481 catgataccc atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg
     3541 ctttttcaat caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat
     3601 catagggaaa aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc
     3661 aacagctttc acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt
     3721 ctgtgagcac ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga
     3781 gtagctgcat ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt
     3841 cccaatgatc gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat
     3901 taccataatt atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc
     3961 caacattagc taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata
     4021 caataaacag attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc
     4081 gcaacaaatc atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga
     4141 ctttttgatc tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc
     4201 gtcccacaga gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta
     4261 cagcttgatg taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca
     4321 tcaaacgttg atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa
     4381 caatccctgc ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt
     4441 agctttctga tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa
     4501 actgattcgt tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc
     4561 atttgtttcc tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat
     4621 aaaaataact gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt
     4681 ccaataaacg gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag
     4741 ctaaatattg ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg
     4801 agaaccacac atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta
     4861 aaaagcgtcc gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc
     4921 tagcatcaaa gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc
     4981 tggataacca cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg
     5041 tacgtgtgat tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct
     5101 ttttgacttt tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat
     5161 tcactgtctg atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga
     5221 ttgcagacag attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag
     5281 cacttacgag cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa
     5341 atcgattgat tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta
     5401 gaacataata aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag
     5461 acaattgact cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca
     5521 tcgtcagcat tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa
     5581 tatctattct catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc
     5641 caaaggatgc cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct
     5701 ttctctttag ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc
     5761 agatccaata gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa
     5821 gaaacgacca tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta
     5881 aaaccatttt tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca
     5941 caccaccaaa accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact
     6001 cttttctttc catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat
     6061 acaacaacat cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc
     6121 ggctttgttg tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg
     6181 cgacttgatt tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac
     6241 tatgttcctt tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct
     6301 aacttgtata cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt
     6361 tagacctgtt ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag
     6421 gataacatga aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt
     6481 ttattaaaaa aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa
     6541 aatgcacaaa aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt
     6601 gcatcacaat cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg
     6661 gaagaactaa gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca
     6721 ttaatagatt tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg
     6781 aatgtcaatg tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg
     6841 gaggaacagc ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta
     6901 gcagaatcag atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac
     6961 atgctgatcg atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc
     7021 actgagattg ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa
     7081 aatccatggg aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta
     7141 gttgccttag aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc
     7201 gagaaagatc ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtgccttac
     7261 aaaaaaagaa gagctgtggc ttttgccgcg gctcttcttt tgtatttttt attatttagc
     7321 ataatcagtt gcacgagttt cccgaataac gattactttg atgtgtccag gataatccag
     7381 ttcttcttcg attcgtttac gaatatcacg tactaaacga acagcgtcta aatccgagat
     7441 ttcttctggc ttgaccatta cccgaacttc gcgtccagct tgaactgcgt aacttgattc
     7501 tacaccggcg aagctgttcg aaatattttc cagacttctt aaacgacgga tgtagttttc
     7561 taatgattca cttcgcgcac caggtcttgc tgctgagagc gcatctgctg cagctacaag
     7621 gacagagata acagaagtag cttccacatc tccatgatga gaagcgatcg cgttgattac
     7681 tactgggttt tctttgtact tggcagctaa ttccgcaccg atctcaacgt gagatccttc
     7741 aatctcatgg tcaagtgctt tcccaatatc atggagcaac ccggcacgtt ttgctaattg
     7801 gatatcttcg ccaagctctg ctgctaagat accagctaat tttgcaacct cgaccgaatg
     7861 cttcaagacg ttttgtccat aactagttct gaatcgcaga cgtccgatga tcttgattag
     7921 atctggatga agtgtatgag caccgacttc aaacgctgcc tcttccccgt attcgcgaat
     7981 acgttcatcc atttctttac gagatttctc gaccatttct tcgatacgcg ctggatggat
     8041 tcgtccatct tgtatcagtt tttccaaggt catccgtgcg atttctcttc ggattggatc
     8101 gaatccggaa agaacaactg cttccggtgt atcatcgatg attaaatcga taccagtcag
     8161 cgtctcaagc gttcgaatat tacgaccttc tcgtccaatg atacgacctt tcatttcatc
     8221 attcggaagg gtaacgactg aaacagtcgt ttctgaaact tgatctgctg cgcagcgttg
     8281 aatcgctaat gataataagt ttttcgcttt gcgatctgct tcttctttgg cacgctgttc
     8341 agattctttt accattagag ttaactcatg attcagctct tcttcagtcg atttcatgat
     8401 gacatctcgt gcgtcctctt ttgataaacc agcaattttt tctagttctt tttgttgatc
     8461 ttcaatcatt ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt ttgaactaag
     8521 tgcttcttct ttactttcaa gtgagttctc gcgtttttct aacgagtcat ctttacgatt
     8581 cagcgtttgt tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta acttactttc
     8641 tctcagctca ctttcaactt cagaacggta tttctgattt tcttccttag cttctaacaa
     8701 tgcttctttt tttagagttt ctgcttcttt ttttgcacta tcgatgattc cagcagctga
     8761 tgattgtgca ccagctattt ctttctcgtg acgtgacttt gcgaccataa accctaaacc
     8821 aagaccgaca attaaaccga tgatagcgag gagaatgttg aataccataa aatccacctc
     8881 catactatct ttttcatttt tatcatctat acagcttcta tgataaatta ttctaaactc
     8941 gtcaatatgc atcttcgcat atatgtgtac aaaaaacatg cacaacttta ttctaatgtt
     9001 tgtaccccgt agtgtcaact ttaaataaac aaagaaacgg ctttctactt attcattaaa
     9061 cgatggtttt taaaataaat gacactagtt tcatataggt aaacctttaa attgagaaac
     9121 ttttctatta aattattctt tgtagatgaa cagatatttt tattttatgt acccactcgt
     9181 ataattaaat aactcacgat ttctttattc aataaaaaaa cggcaggtca aggagagtac
     9241 ctactcttca gaccagccgt ttttgtttat cataattttt attcttctac taaaggcaat
     9301 tcttcttgtc ctttttcatc ttctgggaca gcgacttctt caccaatacc ataagctgct
     9361 ctaactagag cagaaacttc tgccatcatt tcaggatgat tagccatata gattttagcg
     9421 ttttcccgtc cttgaccaat ccgatcttct ttataagaat accaagcacc gcttttatca
     9481 acaatgtctt tttctactgc catgtctaga agttctcctt cttgtgagat ccctaaacca
     9541 tacatgacat ctacttcagc gattttgaaa ggaggtgcta ctttgttttt tacgactttg
     9601 atctttgtac ggttaccaac aatatctgta ccttgtttca attgttctgc acgacgtact
     9661 tccaaacgga tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat ttctggattc
     9721 ccaaacatca cgcctacttt ttcacggatt tggttgatga aaatagcgat tgtttttgtt
     9781 ttgttgatcg aaccagagag tttacgcaat gcttgagaca tcaaacgtgc ttgtaacccg
     9841 acatgtgagt cacccatttc gccgtcgatt tctgctcgtg gaactaaagc agcaactgaa
     9901 tcgactacta cgatatctac agccccactt gatactaaag catcagcgat ctctagacct
     9961 tgttctcctg tgtcaggctg tgaaagaagt agttcatcga tattcacacc taatttttgt
    10021 gcatattgcg gatctaacgc atgctcagca tcaatgaaag cggccgttcc gccatttttt
    10081 tgtacttctg caatagcgtg tagtgcaact gttgttttac ctgaactttc aggtccatat
    10141 acttcgatga tacgtccacg ggggtaaccg ccgacaccta aagcgacatc taacgcaaga
    10201 gagccacttg gaattgtaga gatttgttgg tcgatttttt cgcccaattt cataatagag
    10261 cctttaccat agctcttttc aatttttttc agtgcagcat ctaaggctgc tttacgatca
    10321 tctgccaata cgataatcct ccttatattt acaaatcaga gcaacatttc attatcctta
    10381 tcatatcttt tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg catttatcat
    10441 tttcttaaaa gctgttttct caaaagatca aatccttgca taaccgcatt atatctgata
    10501 gcagcacgat ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt agatgaaata
    10561 gcgatccata ctgttcctgc agattgacct tctaacgtgt ccggtccagc cactccggtg
    10621 aaagctacac tgtaatctgt tcttgctatc tcacgtgctt gaatggccat ctgctccaca
    10681 cattctttgc tgactgttcc atattctgct aatagctccg gatcaatctc cagaaaacca
    10741 gcttttgttt ggagcgagta ggtaacgaat ccgcctggaa atacttcaga tacaccagca
    10801 atgtcgccta atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc tgttacagtc
    10861 tgcttgtttt ctttcagtag ttccactacg acttttgcta gcgaattatc atcgccgtat
    10921 ccataaaaat attcccctac tcgttcttgg attttttctt ctaatgctaa aagagcttga
    10981 ttccctgcct gaacatcatt tgttttcact gtcagtcgta atgtcacttc atttggtttg
    11041 gcatatggtg cgatcgttgg attgatctgt gtctcaatca gatcttttag ctcagtgacc
    11101 agtcgcgatt caccaatccc ataaaagcgc aaaactttag aaattagttt ttcttcagaa
    11161 ggaaaacgtt cctctagtaa aggacgtact tgttccacaa acattggttt caactcatta
    11221 ggcggaccgg gaagaaggat atacgcatgt gtatctgttt gataaaaagt ccctaaagca
    11281 agccctgtac gatttggtag aggaaccccg ccttcgataa tctgggactg ttgcagattg
    11341 tttttggtca tttttcgatg ggttgtttca aaatatgcca ataatttttt gtatccttcc
    11401 gtattttgta tgagagactt accgaggtga gccgcagttg cttcttttgt caggtcatct
    11461 tctgtcggtc ctaatccgcc acataagagt atgagttcac ttcttgtttc agctagagca
    11521 atcaactctt ctaaacgctg ctgattatct cctacaactg tttgataata aacttcgata
    11581 cctagatcag ctagctgttc tgacaagaaa gtggcattcg tattcacgac ttgtcctagt
    11641 aaaagttctg ttccgacagc tattatttct gctttcatta cttcccgctc ccttatatag
    11701 tatacgctgt atttttatat ttttttattt aaaaagaggg caaggagctg tgacaaaaat
    11761 cttgtcacag ctccttgttc caaataaaca gtgttctcct gccaaaacct cggtcataag
    11821 tcacaatata cataaaacat gaaaagctgt tttttgaaat tatcccttgt gactcagagt
    11881 tagacggcag gctcacaacc tctccactaa acgatactta cattgatcct ttgaacacac
    11941 cgctgttttt gatgaagtaa tccacacctg aatagatagt aaagatcaga caagcataaa
    12001 gcatgatttg atctactgga ataccgatca ggctgaatgg gatattattg ataaacagca
    12061 agataatagc aaccatttgt gtagccgtct tgacttttcc cggccaagct gcagccatga
    12121 cttctccatg ttcgacaagt agtaaacgca agcctgtgac ggctaattca cgacagacaa
    12181 tgatcgctac tacccaacca ggggcctttc cttggtcaac caacatgata aaagcagtca
    12241 taactagcat tttatctgcc aacgggtccg caaacttgcc aaaattagtg acaaggcctc
    12301 gcgcacgggc aatttttcca tctaaccagt ctgtgatact agctaaagca aaaataactg
    12361 cacctactag ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt ccccaatcta
    12421 aaggtaaacc tacaaccaaa atgaaaatcg ggatcataca aattctaagt acagttaatt
    12481 tatttggtaa attcacacta tcgactcctt tcttccctat tctacgtgac tatcagcgaa
    12541 gtttccaccg atattattta cttttccaaa tgaatttatt caaaggagta tttttattga
    12601 aattgatctt tttgaattaa cgaagaagga ccgcgacaca gatttgtcac gatccttctt
    12661 ttttatacct acaagtaaac agatgcgtaa actaatttat acgatcattc tgcgtattga
    12721 agagtaaggt tcacattttt tggactctga ttgttttccc ctggattaac tggtacggat
    12781 tctccattcg ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt cgcatttgtt
    12841 gctgtttccg gtaacacggt actctgtgtt tcatttgctg ccagtgtccc ttgataaaca
    12901 taggcattgt ctactagcac acctaaccat acacgattga cagcagtaaa ttcaagggtt
    12961 acaggcttgg tagcgtctgt gatatttact gcgatggcac tgccagaatc ttctcctgta
    13021 gctattgcca ttttcttttc ttcacttgat tttgttgttg attctactgt agaagactct
    13081 ttggtctgcg tgctttgctc tgaggaagag gactcctcga ctgatccttc tactttaagc
    13141 gaagaagctg tgtctccgat tattggatca gcttgacgat cttgccaagt catatagccc
    13201 acgacaacga caatcgccaa agcaaccaat cctaataaaa tgacaggaag acttgtccaa
    13261 aagcgacttg gatgcgattc ttcctgatgc attgcttttc tagaagtact tactgtttca
    13321 ggctgcggac gttcagggac cgatcctgtc aatttgcttt tcccatcaaa aacatcgact
    13381 aaatggtctc catcttctcc tacagcttca gcgtattgcc gaataaatgc ccgcacgtaa
    13441 aatttaccag gcaattggtc aaaatcatca ttgtcgattg cttccaaata acgtttttga
    13501 atttttgtaa tctgctgtaa ttcatctaaa gatatttttt tattcaggcg tgcttgccgt
    13561 aatttttctc cgatggtttc aaaagccact cgttcatctt ctccagtctg tttgattctc
    13621 cgaagcatct gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca aaagtcatgt
    13681 cacagctcct tttttccgaa taaacattgt tctcctgcca actcctcggt cacaagtcac
    13741 aatatccaat aaatataaga agctgttttt tgaacttgtt ccttgtgact cggagttaaa
    13801 cggcaggctc acgacctctt tcttaaacgg tgttcaatca gctgttcctc ggtattagct
    13861 caaaaaccgg caaaatataa ggagatattt ttccgtatgt ttctctaata ctcaggtcaa
    13921 aacgctgatt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata
    13981 agtccaacca agttaaaaac attaagaagc tgtttttact tggctgttct tattacttga
    14041 tgcagggcag ctttttcaca acctcttggt acacggtgtt ccagaagtag cttcttcggc
    14101 aataagccga aatatcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct
    14161 tgaagctgac cacttctgtc acaacctctt tgattacttc gtttgatttt attttaatat
    14221 aagttgatta aaagagagca tgatttgttt tattttctca ctaatttttc caacacaact
    14281 aagaatacca taaaagctga ttttggagga ggttcctact acaagtctaa aacgaaattt
    14341 aagacttttc ctcataaaat actggtttat tttggtctca tgtaaaactc gctgaaggcc
    14401 tcttcactta taaatgcttc cccagctgcc agtacgtctt tcatttggat cgaatcgatg
    14461 atttcaggaa gatcaaataa agttcgatcg ccaaataaac tttgcgtaaa ttgattggca
    14521 atatattcga tcgaatttaa tgattggaaa tattgtccaa gcattttctt cttcagcaaa
    14581 tcgagatttg tttctgacac ttccggatca tcagcgaatc caagaatgat ttctttgact
    14641 ttttctgctg ctttctctgg ttcgtccgta tctccgctga agtccgcaaa gtgaaattca
    14701 cgatccaagc tgaattcaaa accaaagctg tcatcgataa ttccttgatt gtacatcgct
    14761 agatagtttc gagaagtatt gcctaaaatc atttggaaca acagattgat tgctgtttta
    14821 tagcgtaaca gttctgttcc ttcttgcggc aatgtgtcta atcctttaat accaagtaca
    14881 aatttatctc gtgtaatggc tgcttccaat gcactttgtt tgatgatttc ttttgtgttt
    14941 tcagggaaat aacgtacaat ttcttgcttt ggagggaaat tttttgcttc ttggttttcc
    15001 cgaataagtt tcatcaattt ctcaggctcc atcttaccaa ctacaaataa aaccatattg
    15061 cttggctgat aaaacgtccg atagcacgta tacaagtctt gagctgtgat tttgtcgata
    15121 ctttcaaccg tgcccgcaat atcgatatgc aatgggtgtg taggatacaa gttatttagt
    15181 atgccaaaaa acatccgcca gttagggtcg tcttcataca tttggatttc ctgaccgatg
    15241 attccttttt ccttgtttac agtttcttct gtgaagtaag gtgcttgtac aaaatcgatc
    15301 agagtcgtca gattcttttc tacctgatcc gtcgtagaaa acagatagct ggttttcgta
    15361 aaacttgtga aagcattggc ggaagctcct tgtttcccaa ataattgaaa gacatcacca
    15421 tcttcttttt caaacaactt atgttctaaa aaatgggcaa ttccatctgg aactttcacc
    15481 ttttcttttt caccataggg aataaattcg ttatcgatcg aaccataatt cgtactgaac
    15541 aaaccatatg tcttgtgata gtcattttta ggcaacaaat acacggtcaa accgtttggc
    15601 agtacttcat gataaagtgt ttcattgatt tgctcatatt ctgttttatt catttgcatt
    15661 tcctcctgct aaaaagaaga ttgcctgcaa ttcgatttgc tcagctactt gttgtatctc
    15721 tttgatcgtt acgctctcca tacgtttcaa ccattcttca tcagataatt ttgtttccgg
    15781 cagccagctg tccaaaaaag aagcttcgat tgccgcttgc ggagagtcta gagaaagcag
    15841 atattggttt ctcagcattg cttttgtctg agacagttcc aactctgtta tttcaccatt
    15901 tcgcaaactt tctaattgct cgtgaatcag atgaagtact tgatttcggt tcttttcatc
    15961 gataccggtt tggacactca tgaatcctcg gaacgtatcc acgctactag aggcataata
    16021 agctagactt tctttctctc tgacattcat gaaaagttta gagtgaggaa aaccgccaaa
    16081 taaaccgtta aagaccatta gcgcaaaacg ttctggttca tcgtaataca catttgtctg
    16141 ataagctagg ttcagctttg cttgaacgat tggctcacgt acttgtcgtt ccttcacgat
    16201 attcacttga ggttgggtat aaaacagatc tggataaact gtttcccgcg actcaaatgg
    16261 taattgccca actgcttcag ctgctttgtc cggatcaaca tcgccgacaa caaaaatgtc
    16321 gatttgatca tttgccatca tttcttgata gtaggacgct agcgaactag cagtgatctt
    16381 gtccaaatct tcgactacgc ccaaccctgg gatcttttgt tccgggctgt tttcaaaata
    16441 aagttcttgc aaggcatagg aagcaaaagt ttgtttatct tccttcaaac tttccaaata
    16501 tagacgcaga ttgttctttt ccaagtcaaa cgtcaactga tcaaactgat gatttttaat
    16561 atttggataa aataaaacct cttttaaaaa atctaccgct tgagaaaaaa ggtttggatc
    16621 attgacatat tcaccattca caaaactgat ccctgcgttt agccaatgta aatttccttt
    16681 ttttctaact gataatccaa agcttgcgcc ataaagctct gcaagttgcg cacttaactt
    16741 tgtctgatcc gaataattca aactatttgt ttccaacatg ctgctgagaa gtgaacgttt
    16801 cgtgatgact tcttgttgca gacgcgcagt aaaacggata aaaatccgga tcgttttgta
    16861 ttgttttgtt ggtaagacat gaagattcac gccttctgcc aattgataca ccatatttat
    16921 atgctccttt tcacagttga ttgcccgctt actcttttaa tgggtcaacc tgcctttttt
    16981 acttattcct ctgcttgaaa tttataagtt caggcaattc gccgtttttt ctaaatgatg
    17041 ttacacttta tagtatagca tagatgtttt ttatcaaaag aaacagacgg ctaaaagcgt
    17101 atattgtttt tttatttttt atttttcgat atactaaaga caaataaaag aagggagggt
    17161 cattcatgat caaagaattc aaagagttca tcatgcgtgg agatgtcttg gatcttgctg
    17221 tcggcgttgt tattggtagt gcgtttacaa gcatcgtaaa ccaagtggtg caaggcttga
    17281 tcacaccact aataggttgg tttgtcgcaa tgatcacagg tacttctgat ttagaaggag
    17341 cactttcaat tcttgactgg tcgccgacaa aaggcgtcac atttgcgttt ggtaacgtta
    17401 ttagtgctat cattacattt ctgatcactg gttttgtctt attcttagtt gtcaaagcgg
    17461 caaaccgtgc gaagaattta cgctccaaag aagagcaagt ggaagtggaa gaaactccta
    17521 ctgcagaaga ttatcttagt gacatacgcg atcttcttgc caaacaagtg gcagaagaca
    17581 atacacatcc agtagagcaa acaaatcttt ccgatacttc taaataataa agaatctaaa
    17641 aaaaaagaaa gccgattttc agtgtcggct ttcttttttt agattcttaa cttgctactt
    17701 gttccttttt catgtcgata tgtggaatat catcttccaa atacacatct gagattgcct
    17761 tgaatccaaa cgactcataa aaagcttgta ggtattcttg tgcgccgatc acgattggtt
    17821 tttctggaaa atttttctgg atcatgttaa tcgtttcttg aacaatcttt cttcctaaac
    17881 catctttacg gttgtcctga tgaacaagga cacgtccaaa atggatactc tccgcttctt
    17941 catatactcg cgtgtaggca aggatcttcc catcagtatc ttgaaaaaac gtatgccaag
    18001 cttctccatc aatgtcatca acttcttgat aaggacactg ttgttccaca acaaaaactg
    18061 cgattcgttc tttgacgatc gcgtaaaact ctgctgttga caattcttca aatttcttta
    18121 cctgatattc cactaagcaa acgccccctg cttttttatt cttagcatac cgattcgcac
    18181 acaaaaaagc aaacgaacct tacgaatctc aggctcgttt gctttttaaa ctgcttcagt
    18241 taaaaattcg atctcaactg tccgagtaag tacatctgaa tagctgtaag atactcgttc
    18301 aaaagaattt tcttcaggat ccaaatcaac aacgaatact gaaggatagg tttctgttaa
    18361 aacgccgcgg cgttctgttt gacgctttct tcctgtctga gcaaccaaca tgattgggct
    18421 gccgatacga ccttctaaat ctttcttgat tgaagctaaa gttgttggca tacagttcac
    18481 ctctactatc aactatacca catattttta aaattttcaa gtttaccata aaaaaaaaag
    18541 aaatgcaata aaaataaata agaagctcat taagattttt ctaaattatt cctcattata
    18601 aaatttcaag tgttttttta ttttgagttt tactctcccg taagcgctca ttacttgctt
    18661 gtgatccatt gctaaacatt cagccatgtc tacaaaatca acgccttgca tataataatg
    18721 aaaaatttta gcttctattt ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa
    18781 taaaagatgc tgaattgcta aaggttcttg caatgtgatc tctgctaaaa agtctggtcc
    18841 ttctgtctct attttttggt cgattgagac ttcatctttt tgatcacgac gcttcagcgc
    18901 gttctgcttc ctcaacagag aatagatttg attgcttata acgcttttat aataacttcc
    18961 aaagctaaca cctttgcttt tttcgtaagt tctcaatgcc tgatataaag cgatccttgc
    19021 ttcttgttcc caatcctcag aaacataatt tcttaaataa taggtgtttt tcaaacgtag
    19081 taaaagtgga aaatattttt gatacagggc agcaaatgct tcttcgtcac cattacgtgc
    19141 agcgcgcaat aactcctcag acataaaata attcctccat aaattcgttt atggaggaat
    19201 cttatcatca atgattcatc g
//
LOCUS       DAYYOA010000045        19200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000045 DAYYOA010000000
VERSION     DAYYOA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.045"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACHBKA_001441"
     CDS             102..2543
                     /locus_tag="ACHBKA_001441"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM9445135.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ACHBKA_001442"
     CDS             complement(2873..3577)
                     /locus_tag="ACHBKA_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445136.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="ACHBKA_001443"
     CDS             complement(3764..4009)
                     /locus_tag="ACHBKA_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445137.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="ACHBKA_001444"
     CDS             complement(4026..4877)
                     /locus_tag="ACHBKA_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9445138.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="ACHBKA_001445"
     CDS             5043..6047
                     /locus_tag="ACHBKA_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM9445139.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="ACHBKA_001446"
     CDS             complement(6288..6644)
                     /locus_tag="ACHBKA_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9445140.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="ACHBKA_001447"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="ACHBKA_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9445141.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ACHBKA_001448"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ACHBKA_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9445142.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11765)
                     /locus_tag="ACHBKA_001449"
     CDS             complement(11004..11765)
                     /locus_tag="ACHBKA_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9445143.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11932..12564
                     /locus_tag="ACHBKA_001450"
                     /pseudo
     CDS             11932..12564
                     /locus_tag="ACHBKA_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            12623..13483
                     /locus_tag="ACHBKA_001451"
     CDS             12623..13483
                     /locus_tag="ACHBKA_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM9445144.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13668..15983
                     /locus_tag="ACHBKA_001452"
     CDS             13668..15983
                     /locus_tag="ACHBKA_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9445145.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /gene="dnaG"
                     /locus_tag="ACHBKA_001453"
     CDS             16165..18006
                     /gene="dnaG"
                     /locus_tag="ACHBKA_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM9445146.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="ACHBKA_001454"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="ACHBKA_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM9445147.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6226 a         3392 c         4028 g         5554 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
//
LOCUS       DAYYOA010000046        19130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000046 DAYYOA010000000
VERSION     DAYYOA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.046"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACHBKA_001455"
     CDS             complement(<1..703)
                     /locus_tag="ACHBKA_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9445148.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACHBKA_001456"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACHBKA_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACHBKA_001457"
     CDS             1758..2837
                     /locus_tag="ACHBKA_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9445149.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACHBKA_001458"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACHBKA_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACHBKA_001459"
     CDS             complement(3572..3799)
                     /locus_tag="ACHBKA_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9445150.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACHBKA_001460"
     CDS             complement(3902..6490)
                     /locus_tag="ACHBKA_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM9445151.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACHBKA_001461"
     CDS             complement(6510..7169)
                     /locus_tag="ACHBKA_001461"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9445152.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHBKA_001462"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHBKA_001462"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM9445153.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACHBKA_001463"
     CDS             complement(7942..8808)
                     /locus_tag="ACHBKA_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9445154.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACHBKA_001464"
     CDS             8917..9558
                     /locus_tag="ACHBKA_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM9445155.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHBKA_001465"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHBKA_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM9445156.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACHBKA_001466"
     CDS             complement(10999..11898)
                     /locus_tag="ACHBKA_001466"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9445157.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACHBKA_001467"
     CDS             complement(11900..12697)
                     /locus_tag="ACHBKA_001467"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM9445158.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACHBKA_001468"
     CDS             complement(12675..13379)
                     /locus_tag="ACHBKA_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM9445159.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACHBKA_001469"
     CDS             13510..14085
                     /locus_tag="ACHBKA_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM9445160.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACHBKA_001470"
     CDS             14203..14880
                     /locus_tag="ACHBKA_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM9445161.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHBKA_001471"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHBKA_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM9445162.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACHBKA_001472"
     CDS             complement(16943..17941)
                     /locus_tag="ACHBKA_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM9445163.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACHBKA_001473"
     CDS             complement(18167..18820)
                     /locus_tag="ACHBKA_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM9445164.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4137 c         3324 g         6144 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DAYYOA010000047        18603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000047 DAYYOA010000000
VERSION     DAYYOA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.047"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1358
                     /locus_tag="ACHBKA_001474"
     CDS             <1..1358
                     /locus_tag="ACHBKA_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9445165.1"
                     /translation="EVNLEKLSNLLHVCKTTLLNDINNIKKEFEEQIAYTHREKDRYT
                     LYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQV
                     LAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVT
                     ENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTD
                     FQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESH
                     FNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQ
                     LPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQ
                     VAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQN
                     K"
     gene            complement(1450..1815)
                     /locus_tag="ACHBKA_001475"
     CDS             complement(1450..1815)
                     /locus_tag="ACHBKA_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9445166.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1812..2177)
                     /locus_tag="ACHBKA_001476"
     CDS             complement(1812..2177)
                     /locus_tag="ACHBKA_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9445167.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2369..2617)
                     /locus_tag="ACHBKA_001477"
     CDS             complement(2369..2617)
                     /locus_tag="ACHBKA_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445168.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2616..2744
                     /locus_tag="ACHBKA_001478"
     CDS             2616..2744
                     /locus_tag="ACHBKA_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM9445169.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2801..4495
                     /locus_tag="ACHBKA_001479"
     CDS             2801..4495
                     /locus_tag="ACHBKA_001479"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM9445170.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4595..4768
                     /locus_tag="ACHBKA_001480"
     CDS             4595..4768
                     /locus_tag="ACHBKA_001480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445171.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5146..6279
                     /locus_tag="ACHBKA_001481"
     CDS             5146..6279
                     /locus_tag="ACHBKA_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM9445172.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6310..6630
                     /locus_tag="ACHBKA_001482"
     CDS             6310..6630
                     /locus_tag="ACHBKA_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM9445173.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6659..7123)
                     /locus_tag="ACHBKA_001483"
     CDS             complement(6659..7123)
                     /locus_tag="ACHBKA_001483"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445174.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7255..8508)
                     /locus_tag="ACHBKA_001484"
     CDS             complement(7255..8508)
                     /locus_tag="ACHBKA_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9445175.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8537..8854)
                     /locus_tag="ACHBKA_001485"
     CDS             complement(8537..8854)
                     /locus_tag="ACHBKA_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9445176.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8880..9200)
                     /locus_tag="ACHBKA_001486"
     CDS             complement(8880..9200)
                     /locus_tag="ACHBKA_001486"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9445177.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9664..11079)
                     /locus_tag="ACHBKA_001487"
     CDS             complement(9664..11079)
                     /locus_tag="ACHBKA_001487"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9445178.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11096..12412)
                     /locus_tag="ACHBKA_001488"
     CDS             complement(11096..12412)
                     /locus_tag="ACHBKA_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9445179.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12562..13260)
                     /locus_tag="ACHBKA_001489"
     CDS             complement(12562..13260)
                     /locus_tag="ACHBKA_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM9445180.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13549..14538)
                     /locus_tag="ACHBKA_001490"
     CDS             complement(13549..14538)
                     /locus_tag="ACHBKA_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9445181.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14618..15451)
                     /gene="msrA"
                     /locus_tag="ACHBKA_001491"
     CDS             complement(14618..15451)
                     /gene="msrA"
                     /locus_tag="ACHBKA_001491"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9445182.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15626..17260)
                     /locus_tag="ACHBKA_001492"
     CDS             complement(15626..17260)
                     /locus_tag="ACHBKA_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM9445183.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17247..>18603)
                     /locus_tag="ACHBKA_001493"
     CDS             complement(17247..>18603)
                     /locus_tag="ACHBKA_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445184.1"
                     /translation="GEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIP
                     AVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKN
                     DIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQA
                     LNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKT
                     PISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKEN
                     YAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEK
                     GKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAG
                     ENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNEN
                     K"
BASE COUNT         5865 a         3682 c         3113 g         5943 t
ORIGIN      
        1 aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt ttgcaaaacc actttactca
       61 atgatatcaa taacataaaa aaagaatttg aagaacagat cgcttataca catcgggaaa
      121 aggaccgtta cactttatat ttttcagaac atatacctcg atgcaagatc atgcagcaat
      181 tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta tttggaagaa gatgaacctg
      241 attatcttca attaactgaa tgtgaattca tctctgtcag caaagcttat tcattaaaaa
      301 aacaagtatt agcttacttt aatgactgcg ggatcgaaat cgaccgctac tcccctcgat
      361 tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta
      421 attcttggga gctacctgaa tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa
      481 cggaaaatag tggcagattt tatgataaag aaaataaaga aatcttatcg attggttttg
      541 ctatcagttt cctacgccag caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag
      601 aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc atgggaaaac actgatttcc
      661 aaacctatta taaaaaagaa gaattcgcct ttattttgac actattcaat ctttgtaatt
      721 atggtttcca ttcttatcag ttgattgcgg aagactttca acagctacac caagtgttta
      781 ttgacaatac tcccgaaata aaagaactag ttgctacctt tgaatctcac ttcaatcaag
      841 aattattcgg aaaccaaccg ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg
      901 ataactatca attattcatg ccagaaaaat tttatctttt aaatgaggaa cagacaaatt
      961 tatataaaga agtccagaca attttttcca gctggtccag ccagttacct tatgatctac
     1021 ggttgaatcc taattgcatg cgggcttttg tgatcgaatt gagcggaatt ctccgtctga
     1081 caaaagaaca tctgactatc tatatcgtca caaattcgga tgttcattat ctgatttatc
     1141 gggaagcctt ggaagctgta actactttcg attttcaagt tgcaccaaca atttattcaa
     1201 gtatatctga tataaagaag tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta
     1261 cattatatac accagatgcg gtccaatatg aaaatattat tcctatttcg atcaatacga
     1321 tcgatcgagc tatcatttca gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt
     1381 cacagcctct tatttgttga agatgcccat ttctgtaaca atctcttgat acacgtcagg
     1441 ctcacaacct cacttatttt ggacatctag gggtccgatc tctttattta aaaaataaga
     1501 aatgatggtt cggataacta ccaacgccgc tagttggaaa atatcctgca aggtaggttt
     1561 gataatcgat tcgatgatcc cagctgaaac aagaatttcc aaacttagaa gtatatagcc
     1621 tcccaacatc tttttaatag cattgttctc gcttactcgg tcgctttgca cttttgtatg
     1681 gacaaataaa tatttcaaaa aatccttgat tgatagacaa acaccccaaa ccaataaaca
     1741 gatagcaaag atatcaagtc ccagtacaag tttttcgaaa tatggaagaa gaaactgcat
     1801 caactctgtc gtcatcctac ctctcctctt ctttgttttt ctttgtcttc tatttcctga
     1861 ttcaagaaat aagaaatgac tgttcgaatg ataataataa gtgcaagctt caaaatatct
     1921 gtccaagtcg gcttgataac tgactcgatg atatcagcaa cgactagtac ttccaagctt
     1981 aacaaaatat aacttgctag cagttttctt gtatctgcat tacgagtatt acgttctgca
     2041 tagtttttct tgacatgaga ccaatcaaaa agtttgaata gaacaagaac gatcccgaca
     2101 attaagatac cgatagaaca aatatttaaa atagcaatga tccattctag aaattcaatt
     2161 aaatttgagc gaaacatata cgctacctac tttcttttaa tttgttccta ttcttttatt
     2221 gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt ctaatgataa cggttcgctt
     2281 aaatttttct ttttcactct cccatttttc tccctccatc cataaatcgt ggaagtatcc
     2341 aaaacaacgt agcaagttaa aataaggctt agtccgtcat cagcttttca aagaagcact
     2401 gtctcggtgc tttttttgta cccaaatttc taaaaataga accatacacc tcctcaaatc
     2461 ttttcttctt acctacccta tttttattta caggaaaatc agacacttct tcttttttgt
     2521 caaaatttcc tttttcttct cgtcctatca tttggcattc taataaaaac gaactatcct
     2581 tggatataga aagagaaaac aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg
     2641 gcatcggttt aggtttgctt gcccttggcg ctgcatatct aggaaaaaag acaggactat
     2701 tcgaagatga cagtcattta tatgatgaat tcgaatcaat ctagcatcct atttagttga
     2761 atcactacgg acatacttgg aagacattgg aggaatcaca atgagaaaaa gtaaagcaat
     2821 aatgattggt gccggtattg ccaatatggc agcggctgtt tatttgattc aagaaggcaa
     2881 atggaaaggg gatcagatca ccttttattc attggatgat cacggttcaa atgatggtgc
     2941 accgaccgac actgtagtgg atgaatattg gaataaaaac catccattag aaaaccaaaa
     3001 aggctatatt gctcgaggcg gtcgcatgct taattaccgt acctatgtag atttgatgga
     3061 tctgttaagt cgaatacctt ctgttactga gccagggatg actgctgaag aagatacgag
     3121 agattttgat gcaaaacacc gaacctttga caaagcacgt ttgatggaag gtggcaaagg
     3181 aatcattgat ggcggaaaac tgggattcaa taataaagac cgtattcttc tgactaaact
     3241 gattgctatg cctgattcag aagaagaaaa actagacaat atcactatcg aagaatattt
     3301 caaagatgat ccacactttt ttgaaacaaa cttctggttt atgtgggaaa caacatttgc
     3361 tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga
     3421 attcactcaa atcgaacatc tagtgggcgt caatcgaaca cgttacaatc aatacgaaag
     3481 tattatgctt cctttgatcg agtacttgaa agaacaagga tgcaaaatca ttttgaaccg
     3541 ccgtgtcact gactgggaat tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg
     3601 tttgaaaatt accaatacac tgactcaaga agaagaatac gttgtggtcg atgaagatac
     3661 agcagttatt ttcactaatg ggtccattac agattcagct actttaggag attacgaaac
     3721 accagctgta gaaaatatgg attatggtgc agcttctagt ctctggaaaa aagccagtga
     3781 acgtttctac aacttgggaa atcctgacaa attctttgct gatcgagatg caagcgaatg
     3841 ggtcagcttt accctgacga cgaaaaacca tttgttgttg aatgaaatca cccgtatcac
     3901 gactcaagaa ccaggaaatg ccttgaattc atttatttca accacaccga tcacgccgtt
     3961 aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat caaccgcatt ttacgtctca
     4021 gaaaccaaat gaaacagtta tttggggtta tttcctttat ccaagacgac gtggcgaatt
     4081 tgtggataaa cagtatatta aaatgaatgg aaaagaaatg ctagaagaac tgattggaca
     4141 actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa aaagaaacag aaattttcga
     4201 cagcgtaata aataatatcc ctgtctatat gccttatgct tccgcgttgt tcaataatcg
     4261 tgccaaaagc gatcgaccaa aagtcattcc taaacattcg accaatctgg cctttacagg
     4321 agaatttgtc gagcagcctt atcagatgat ctttactgaa caaagtgctg tacgttctgg
     4381 agaaatcgct gctttccatt ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg
     4441 ttacgacaaa gatattccta ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag
     4501 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca accaggctaa aacagcttct
     4561 tatgtttttg atttggttgg acttattacc gcagatgcaa gcttttgaac accgtttacc
     4621 aagaggttat gaaaaagctg ccctgcatca agtaataaga gcagccaagc taaaacagct
     4681 tcttatgttt ttgacttggt tagacttatt accgcagatg caagcttttg aacaccgttt
     4741 accaagaggt tatgaaaaag gcgtttagtt tcgagtatga agaaaaattt tgtgaaaatg
     4801 gttcctcacg tttttgcata aatttgactt aataccgaga aactgatccc ttctgtcata
     4861 acctcttttt atttatgtct gtttcttttt gaaaacaact attttttcaa cgtttactct
     4921 cttgccaatc caacatcatc taacggatac aactatttca atgattacca ttacactaaa
     4981 aaatcggaaa cacacctctc cgaacacaaa tacataagac tatagaatgt ttcatttaag
     5041 aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt aaaatgaaat tatctgtagt
     5101 actaataatg cagacaattt tatgaatctg aaaggagcat ctccaatgaa agcagtcact
     5161 tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg atccaaaaat attagaacca
     5221 acagatgcaa tcatccgtat cacagcaaca gccatttgcg gctccgatct gcatctttac
     5281 catcatggtg aagcagtttt ggaaaaaggg tatgttgtag gacacgaacc aatgggaatc
     5341 gttgaagaag tgggatctca agttcaaaca ttgaaaaaag ggcaacgtgt ggttatccct
     5401 tttaatatta gttgtggtca ttgtcatttc tgcctgaatc acatggaaag ccaatgtgat
     5461 gaatcaaatc cagatcagtt atatggcggg cttttcggat ttggcaagtt gaacggtaat
     5521 cattggggtg gtcaagcgga atatctaaga gttccttatg cagactctac ttcatttgtt
     5581 gtaccagata atgatcttcc tgatgaaaaa gttttatttc tatcagatat tttgcccaca
     5641 gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg atacagttgt gattctaggt
     5701 tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta tggctggagc agagcgcgta
     5761 atcgcagtag atccagtaca gcaccgtcta gataaagcgg ctaattacaa tggggttgaa
     5821 acttatttct tagaagacac ggcacaagct ggaacagaat tgtatgaact ggctaaaggt
     5881 ggcgcagatg tcattatcga ttgtgtagga atggacggat tagagcctgt caaagaaaaa
     5941 gcgaaaaatt tagtaagctt acaaagcggc acaatctctc cattacagat ggcatcgcaa
     6001 gctgtcaaaa aatttggaac agtccaagta actggtgttt atatgactcc agcttcttct
     6061 tacccgctgc aagaattttt tatgcgggat atcgctgtca agcatggaca ggcaccaatt
     6121 atccatctaa tgccaaaaat ttacgcaatg attgcagaag gactattcga tcctacacaa
     6181 atcattactc attcgctgcc actatcagaa gcagcacaag catatgagat ctttgacaaa
     6241 aaagaagata aaaatataaa agttgttttg aaaccttgaa ttcagtaaaa aatacaggtg
     6301 attttcttta tgcccaaaat caaacgaaat acccatgaga tcgaaatagg aaaagaagaa
     6361 gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc aaaatcgtta cacattgatc
     6421 tcactggctg tcgatttatt aaccgggatt ttctatattt ttggaagcct tgcttcctta
     6481 actaaaatcc ccgatcgata tggcacctat ttttatttgg ctggtgctat tttcttgacg
     6541 attcgtccta ttctcagaat cgtaaggaac atttttattt acgacgaaaa aaaagcaatg
     6601 aagagaacaa gcaaaaaaaa agaggcatga gaatttttca aatctcatgc ctcttttttc
     6661 accctatcgt atcactttga tacagacgag cggtcgtccc actttcttcc ttgaccattg
     6721 tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg atctgtaatt aagtaaagtt
     6781 tttcgtaccc aagttgatgc atttcttttc tgatttccgc taatatccgc ttggctatcc
     6841 cctgcttacg cttttccggt tcaacaaaca gggcacaaag atttggtgtc agatcagggc
     6901 ggttatggaa gtcgttttca atgacgcccg ctcctccgac gatggtttct ttttcatcta
     6961 agaccacata ccattgagga atactttcct tattagcgat tccagtacga atactttcct
     7021 catacacaac agcaggaact ccccattttg atgaaaacca attcgcgctt tgttttacta
     7081 attctgggtg ttctcttaat ttgatccagt tatatgtaac caatgtttaa acctccgact
     7141 aaaataaaac gtttacaaaa attatacaag tctttatgaa aattagcaat gacaacaata
     7201 gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc cgttttttga ttaatcaagc
     7261 atctaaagct tcttttcttc tttcttggat cgtagctacc ttgacgaacg gtagatagat
     7321 caaaatagaa agaaccaaat tgacagcggc tagtacacca cctgcaatac tttttgttgc
     7381 caatacaccg ccaataattg gtggagttac ccatggaggc ataacggtag ctgctggcac
     7441 taaacctgta gaagtcgcaa agtaagcaac actaactaag accattggtg tcaaaataaa
     7501 tggaatgaac atgatcgggt tcaacacgat aggtaaccca aacatcgttg gttcattaat
     7561 attgaagaca cctggcgcaa tcgacaagtt tgttacaacc atatatggtt tgtttttacg
     7621 tcctactaaa taaatagcta acagtaaacc taaagttgca cctgttcctc ctaagttgac
     7681 aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg ctatgtccag ctgcgattgc
     7741 attaacgttt gcttcaatgg ctggcgcatt gattgtttgc atcaaaggat cgaccatatt
     7801 cgctccgtgt aaaccaaaga accataagaa tggtgtgata aatgcaagca gtaaggcaga
     7861 tggataagag tttgcaagtc ccataaatgg ttcttggacc actgtataga aagaacctac
     7921 gatatcatca acaccgaatg cagcaaaaat tgcagcaact aaagcaaacg cagagatagt
     7981 gatcattgct ggtagtaatg aagcaaatgc ttttgcaact gccggtggca caccatcagg
     8041 cattttgatg atcaattttt catttccgct taaacgtaca aagatttctg ttgaaataat
     8101 cccaacaata atagcaataa acaaaccgtt tgagctcata cctaatgctc cgcctaaagc
     8161 gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg tcttttcggt agccgttagc
     8221 taaatgataa gcaagcaaga aagcaatcaa gacagaaaga atggcaaatg taccgttcca
     8281 agcagcagcc ccgaatcctt tccatgattc accaccaaaa attgagttca tcaattcttg
     8341 gaacccggga atcggtaagt tattgatcat tactgctaat gctcctaaga tcattaacgg
     8401 catactcacc ataaatgagt cccgaatcgc aactaaatgc cgttgtgcac caatttttga
     8461 tgctacaggt ataaaatgtt tctccataaa tgcaacaaat ttatccataa atcttccctc
     8521 ttttctttac tgtagattac gcgttcaatc gacgatacag attaatgatc tcttttgcta
     8581 aatctgtaaa agctatagat gtcatcagat gatcttgact gtggaccgtc aaaagcgtga
     8641 cttcaacgtg atttccttgt gcttcttgtg tcagcatctc tgtttgagag tgatgtgctt
     8701 ggactaaaga ttcctctgca tctttgattt tttcatctgc taaagtgaaa tcaccttttt
     8761 tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt tccgccaaac atgatcagac
     8821 ccatgatggc ttctagattt ttttgatctt ccatttattg tatcctccga taatttttat
     8881 tatttgttta ttagtttttc tgcttggtcg agtacttttt caccattcat catgccatag
     8941 tcagccatat tgattacatc taaaggaatg cctttaggtg ctagcttttc agcaaattga
     9001 ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt ctacattttt agattctagt
     9061 tgattatccg catctgaagc agatacagca aagatgtctg cttccattcc ttttgcttct
     9121 gctgcctttt gcatttttgt gactaggaga cttgtactca ttccagctga acatacaagc
     9181 atgattgttt tcttttccat tttaaacacg ttcctttcta tacttacact tgtatataag
     9241 caagtagcgt gccaacttta aaagcgcttt aaaaagttga tatcatcaca tttaaggtct
     9301 ataccaagtt gcatacacac taaaaaagaa agcgcacact aaacacacaa attaattttt
     9361 attacctttt ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt gtcacaactt
     9421 ttttcaaata aacggtattc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa
     9481 aacataagat gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca
     9541 caacctctca ataaacggtg ttcaatcagc tgaccctggg tattagctca aaacgctgga
     9601 ttcacaacct cttttatttc aaataaccag taaatacaat gaacatctac aaaaaacttc
     9661 tatttattta tagatttcgt caggcaaatc caattctctc ttatcaatca aagtctgata
     9721 ccacgaagca gattttttca atgttctgct tcgttcatcg ttcaattcga ttcgaactaa
     9781 tccatatcga ttcttaaatg cattcattgg tgacacacag tcagtgaatg cccataacat
     9841 atagccttcg caatttgcgc cttcttctgt tgctttgatc aagtaatgca aatgctgtcc
     9901 gataaattcg atacggtaat catcttgcac gattcctttc tcatccatat actgttcttc
     9961 atattctctt cccataccgt tttctgttat cagccaaggg atattatgat aattttcttt
    10021 tagatacatg gctatatcat acatgatttt cggatagatt tcccatcccc tggaattatt
    10081 cattttcttc ccaggtaaat caaagacttc ataatatcgt tcaggatgga aaggtgtctc
    10141 ttcattccat tgataacgag gggcgcgaac gcgtttagga taatattgat tgattccaag
    10201 gaaatctact gtatgatgct gaatcgtttc taaatcctct gttgcaggat cgaaataaat
    10261 cgattgtttt tgacacaact ccaggaactc ttctgtgtag tatcctttta ccatcggatc
    10321 aaaaaatact cgattataaa acaaatcata catataagca gctttttggt cttctggaga
    10381 attggatcga gcatagacca tttcaggatt taaaatacag ccaatccttc caccataatt
    10441 tccgctgcgg taaatctcta ctgctttagc cgttgctaaa actttatgat aattccacat
    10501 catccacttt tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt
    10561 ttggggaacg attggttcgt tgaatgtaaa ccagtttttt accctatcac cgtaacgatc
    10621 aaaggcaatt tgagcatact tagcataaaa atcaactact tttcttgaag accagccatc
    10681 atatttttcc aaaagaaaag ctgggacttc ataatgttcc aaacaaatca tcggttctac
    10741 gtctgcttcg atcaaagagt cgatcatttc acttatatgc tgagcatatt cttcatctac
    10801 tatacagttc tcataatcag taaaaaaacg agcccaattg atagaagtcc gataatgtgt
    10861 cagatggatg gatttcatca gatccacatc ctgctggtat tgttccataa aattagttgc
    10921 tacggctggg ccatacccat tatgccaaac aaaatgttca tttttatacc agctatctat
    10981 aaacgaatcc tgtccttctt tttttccttt ccaaccttcc gtttgccaag cagaagatgc
    11041 agcaccaaga ataaaatttt ttggaatagt aattttcatg atttcctcct gttttttaga
    11101 tcccatcatt ttccacacga tttgctactt ttacaaatgg cagatagatc aaaatagaaa
    11161 caataataca aacaagttgc gtaataactg caccgatatt tcctgctgtt gccagataag
    11221 cattgacgat cggcggcatt ggccaaggga ccattacaac tgctttcgcc gcaaatccta
    11281 aagcggttgc aaaataacca atcgttccag tgataagagg cgttaagata aacggaatag
    11341 ctaaaattgg attcagcatg atcggcatac caaaaataac tggttcattg atgttgaaga
    11401 tcccaggagc aagggataac ttggtgattt gtttcatatc ttcccgtttt ccaactaaca
    11461 atactgcaat cagtaaaccg atcgtgactc cagacccgcc catactcata tacatatccc
    11521 aaaatggcat agtaataatg ttcggtactt cttttccagc ttcaaaagct tccgtattta
    11581 cagcaattgc cgctaaaagc aacggttccc gtatcggctt caccatttga tttccatgga
    11641 taccaatcac ccaaaagact tgcgcaataa acatcaatag caaaataccc ggtaaccctt
    11701 gtacgacacc ttctaatggg cgttgtacga cattgtaaat gatatcataa aggtacattc
    11761 ctgtaagttc tttgatgata aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg
    11821 ggaataaagc agaaaaacta gcagaaacat tcgttggcac ggtatcaggc attctcagct
    11881 gtagtttctt ctgtttcctc aaccagcaat aaagttcaat agaaaggatg gagaccaaca
    11941 ttcccaaaaa taaccctttc gtatctgtat attgtttagc caatacgttt tctactataa
    12001 cagtctgatt ttcattcact aattcataaa cagttggatt aacagataaa taagacatca
    12061 ctgcgatgat accagggaat acaccatcca ctttattcag cttggctaat tcgatcccta
    12121 tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac atagctgatg gcagatgcga
    12181 taggctttaa ttcttctaaa aaacgcaaga aacttatctg tgctaagcca ttttccgagt
    12241 caaagaccat tgctgaaaac aatgtgccaa aagcacccgt aataattacc ggaatcaatg
    12301 cagcaaatgc gttttttatc accatgatat accttaaatt attgattctc gttgccacgt
    12361 tccctaaaac attggctaat ttatccataa aactcattcc agatcccctc attcctgctt
    12421 cttctccttg acgtcatctc tttttgtcta ttagtcagcg cttacatttc gatgaacaga
    12481 aactatttac tcctcttttt taccaaatat aaaacaaaaa ttcatctgat gtatttatca
    12541 taaaatacat cagatgaact gtcaattagt ttctttttct cctattattt tctgtaattc
    12601 tctccgattt attgccatat ggacagtcat tgcatcaaac gcagcaagtg cattccgcct
    12661 ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc tcgattttca gatgctggat
    12721 atcataattt ttattaaaca aagcaattga ttcattaatg attgggataa tatgcagcaa
    12781 ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta attgaacatg
    12841 ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc gttaattttt tcatttggac
    12901 gatttgattc ttcgttgcat attccgctgc caaacctgcc atcttaggct ccaataaata
    12961 tctcagttca aataagtctc gtatcatccg atcctgatct tcaatcaaag aaaaaccgag
    13021 tgggtcattg gcaatccctc gcttatcact gacaaatgtc cccgctcctt gccgaacttc
    13081 aaggatattc ctggaagcta gagcccgcac agcttctcgc accgtgcttc gccccacctc
    13141 taaataatcg gacaattcat attcattcgg cagtttatct cctacttgca actgcatttc
    13201 ctttaaatat ctaaggatct tgtcagtagt tacttctact agggttttat tttcttccaa
    13261 accacatacc tccttttcat ctgatgtatt ttttcgtaat tattataaca cgtttgaatc
    13321 atctgatgat tttttgtata caaaattgat taatccgttc atagacatac aaacgaagaa
    13381 gatatttacc ataaaaaaga ctaggataaa attcactcct agccttcgat catttttatt
    13441 ttgaacgaca aaaaatatga tgttcaataa cagacgatct tcattcgatt cttgatgtca
    13501 tcttttgttt tttttaatat atggataaaa agtttgatta catcgtttct attctagaat
    13561 ttggtcgatt ctcaattctt cttctggtga gaaatctaaa ttatccatca ttcgaacact
    13621 ttcttgtaac tgacttaacc gacttgctcc gaccagtaca cttgtaacag atttttgtcg
    13681 caaattccat gcaagggcca tttgcgctaa tgattgtcct cgtgatacgg cgattgtttg
    13741 cagtgctttg attttttcca gagttgatcc aacttgttct tctgaaagga atggtatttc
    13801 cttacgatgt gctctagaat cttcaggaat accatgtaga taacgatttg tcagcaaccc
    13861 ttgagcaagc ggactgaacg tgattgcccc tagcccttct tcttctaata ctggagatag
    13921 atcgtcttct agtagtgcac gagaaaacat attgtagcgc atctgatgaa taatgaacgg
    13981 tgcttccttt ctttttaaaa tttcagtcat tttttttgta tcttctccat tatagttgga
    14041 tataccaata tataatgctt ttccttgtct tacaagttgc atcaaagctt ctgctgtttc
    14101 ttccaaaggt gtatcagggt ctgggcgatg atgatagaag atgtcaacat aatctagccc
    14161 cattctttgg agactttggt cacaacttga aatgatggat ttacgagagc cccattcccc
    14221 atatggccct tcccacatat gataacctgc tttgctagaa ataatcagtt catcacggta
    14281 ggcttgaaaa tcttctctaa aaatacgccc aaagtttttc tctgcagctc cagcagggcc
    14341 tccataatta ttcgcaaggt cgaaatgggt aattcccatg tcgaatgcgc ctcttaaaat
    14401 ctctctttga ttatggatag gatcataatc accaaaattt tgccataatc cgagagataa
    14461 aatcggtaat ttcaaacctg aatgaccgga tttacgatat tccatttttt cataacgtgc
    14521 actatctgca atatacaatt tttctcctcc tcatcaaatc tttttaaaac gctttctttt
    14581 ttctattttt agtttactac attttaaaga aaaggagtta atcaaaatga ttttttcgta
    14641 tatttttttg ccatttgttt tttactcgtt gatagatcag aaattgatta cgtgcttgct
    14701 ggattttttt ataacgtttc ggttgttttt tgtaaaattg ctgatgatag ttttctgctg
    14761 gccaaaacgt accggctggt tcgatttttg tgacgattgg ttggtggaaa gttcctgaac
    14821 tgtctagttt ctgttttgat tgttccgcta attctttttg tcgttcgact tgataaaaaa
    14881 tcactggacg atattgcttc cctctatctt gaaattgtcc aaatgtatct gtgggatcag
    14941 taatttgcca atagattgtc agcaactcgc tataacttat ttctcttgtg tcataaatga
    15001 tctccacagc ttccacatga cctgtgtaac caccacttac ttgatcataa tttggttttt
    15061 caacatgtcc gcccgtgtat cctgacaaaa cagataggat cccttttttc gattcaaacg
    15121 gctcgaccat acaccagaaa catccccctg caaatactgc acgttcttga tagtcagcat
    15181 cttgcatggc atgcttggaa aagtcatatt tttgctcctt atcaaatcct tccgtaatca
    15241 ataaataaaa gtccattaca tcaggcgtca gattcattcg gatagctaat ggacgtagtg
    15301 ttgcttctag ttttgataac tgttctttaa cggatatatt ttctcttatg tcattctttg
    15361 tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg attcaaaatc agattataaa
    15421 gtgtttgaat gaatatttgt tcctcatcca tagattctcc tcctttccga agaagacaag
    15481 ctagtgactt tagctgtaat ttatcataaa gtgaggaaat aagcgattac ttcatccccc
    15541 tacagatttt tcgtataaaa aagagagaat ccttatcgac agattctcct ttttaatcaa
    15601 tttattttat aaatgaaaag ttaatttacc ttttatggaa aactatttta aaaaataaac
    15661 ataaatatgc agttaatgta acaagataag aaataatagt cgttactgta taacttgtgc
    15721 ttactttaat atcagcaact cctttgactc cgcttatttg taccaatcca ttagcattca
    15781 cctgctcctt tacaggttga tgatttattt ctacattcgt tcctaaataa gcatatacag
    15841 gaagtgtgat tacttggctc ttctcattag ttactctata tatgatagaa ctaccagttg
    15901 ttttataagg aaggtttacc cattgactgc caatctttgc ttcgtgtttt attatatgct
    15961 ccttattctc caatgaagcg ataggagaat aatcatataa aaaataatcc attcctttag
    16021 tggtatacgc ctctactgtt tctttgttta caacaatatg tcccggctga gaaattattt
    16081 tttgattgaa atttttttta ctagaaacag ttaaagtgat taataacaaa acagtcataa
    16141 ataaataaaa tagttctctt gtcttattgt atcttctatt tttgaaaaat atcatcgtta
    16201 atatcagaga agagaatatt atttgcaagc ttaagatccg ccaaggaaat tgtaacaatt
    16261 tggctggcgt attttgcaga agataccatg gcaccaaact agttgtagct agccaagtca
    16321 aacacgtacc ataataaatc tttttttcta tgtttgttaa ttttttaaat aaaatcaggg
    16381 gaatacataa agataacaga ccaactagtc ccattgagta agtagttaag tcgttgttca
    16441 tagcttcagt cagactatcg aacacattag atgcatgagc atataaattt gtcacgctag
    16501 gtttgttgat tttttggtag agcatttgtt caaacatcgg ataccaaaaa gcactcgtca
    16561 gaaccaatgt cattcctgcc gcactaaata attttttaaa tatagcaata aaatctgcat
    16621 tcttgaaacc tttagaaacc aaacgaataa aaataaaaat gataattaat gctgtactca
    16681 ttaatgttga tagtacatgg ctgtacacta gcaaactcat actcaatgct agctttacag
    16741 acggatactt ttcttctttt ccagagatga tttgataagc ataaaataat acagggataa
    16801 caagtgttat tgcgatagct tcggcaattg cggaacgata gtagatgtct actgtccgat
    16861 aagcagagaa aatatagaga caactaaata aaaatgccgc ttttttttgt tttaaaaacc
    16921 gctcgccata atgataagct acgagtgttg tacacaagtt caataaccaa acgtaaataa
    16981 tatagcttac aatcaaattt tttgtcatcc aatataacat tacagctgga aaaaaagtca
    17041 gaaagggata aaaataattt acaccatatc cagtataatt gaatgtctca taattaatcg
    17101 gactggtaaa aatagtcgat aaccccttga ttcgagataa gtgaaaagaa agatctccag
    17161 tggtataaat actctgtgta tttttgaaaa agatgaaaga atatatacta ctaactataa
    17221 ttaatccact caatattact acttttttat ttattttcat tcttcttgtt tatctccttt
    17281 ttagtcacat atcgagaatc tttcaactta ttaggtgcaa atcgtttaaa acttgaatac
    17341 accactaaac aataagtcag aatactgatc cataaagcca tgagagcagt tggagtatat
    17401 tttgttgtaa tttctatctt attttctcca gcatttacat ccacattcac taagccatgt
    17461 tttttataag agtcatttac gactttaccg tttactttta cctgtgtacc taaataagca
    17521 tatactggta ctgtcacttt ttgctttttg ttgcttgcca ctgtgtaagt aatgttatta
    17581 tctgcagctt tatacttccc tttttcccac gattctccta cttttacttc atgtttttct
    17641 aagtgaggcc tttcttttat cgcttctgtt ggggcgtaat cgtataatcc tcccatatct
    17701 gatgttgtat aacgtgggag tgtttcttca ttgataacaa tatgaccgtg ttttgattgg
    17761 ataacttttg cgtaattttc ttttgctgct atagacgtga cgagtagaaa caccacagta
    17821 gcgccaatga ttaaccatga atatttttta ttcatagtct ttttcataaa aacaagcgtt
    17881 atgatcattg aagaaaaaaa gatttggata cctaagatac gccaaggaaa ctggagtaag
    17941 cttatcggtg ttttttgaaa caaaaaccat ggaaataaat tagttacgca tacccatgta
    18001 gaaactacgc caacatagat gattttttct tttttctcca tttctttcag tatgaataaa
    18061 ggaaaaatca acgcaaccaa tcctaataac cccatagtgt atgttgtaat atcgttattt
    18121 aacgcttgaa tgatactatc tgaaatagaa agtgcttcta gctgcaaatc tgttttgaag
    18181 ggactgtgta tttcttgata attcatctgc tgcaacatag gataccaaaa atagctagtt
    18241 aatgcacaag tcaaaaaaac agcctttact aaattcacga ccacaaattt tatatcgttt
    18301 tttatttgct tgcttaaaaa caaacgaact ataatcatac aagcaatcaa tgcactacac
    18361 aatagtgtag ttaagacatg agtatacaca agtagactta tactgacaga taaagcgatg
    18421 actttttttt ctctattgaa tatcaatcca taagcataat ataaaacagc tgggataatc
    18481 gtaagagcga ttgcttctgc aattgcggat cgataataga tatcaactgt tcgataagtt
    18541 gcaaaagtat ataaacaact aaataaaaaa gcagcatcta tctttttaaa aaacttttct
    18601 cca
//
LOCUS       DAYYOA010000048        18541 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000048 DAYYOA010000000
VERSION     DAYYOA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.048"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(293..1585)
                     /locus_tag="ACHBKA_001494"
     CDS             complement(293..1585)
                     /locus_tag="ACHBKA_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9445185.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1582..2205)
                     /locus_tag="ACHBKA_001495"
     CDS             complement(1582..2205)
                     /locus_tag="ACHBKA_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445186.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2208..3044)
                     /locus_tag="ACHBKA_001496"
     CDS             complement(2208..3044)
                     /locus_tag="ACHBKA_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445187.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(3061..3948)
                     /locus_tag="ACHBKA_001497"
     CDS             complement(3061..3948)
                     /locus_tag="ACHBKA_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445188.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3956..5191)
                     /locus_tag="ACHBKA_001498"
                     /pseudo
     CDS             complement(3956..5191)
                     /locus_tag="ACHBKA_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(5874..6557)
                     /locus_tag="ACHBKA_001499"
     CDS             complement(5874..6557)
                     /locus_tag="ACHBKA_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM9445189.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6575..7192)
                     /locus_tag="ACHBKA_001500"
     CDS             complement(6575..7192)
                     /locus_tag="ACHBKA_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9445190.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7202..8026)
                     /locus_tag="ACHBKA_001501"
     CDS             complement(7202..8026)
                     /locus_tag="ACHBKA_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM9445191.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(8019..8579)
                     /locus_tag="ACHBKA_001502"
     CDS             complement(8019..8579)
                     /locus_tag="ACHBKA_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445192.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8753..9709
                     /locus_tag="ACHBKA_001503"
     CDS             8753..9709
                     /locus_tag="ACHBKA_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9445193.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(10014..10970)
                     /locus_tag="ACHBKA_001504"
     CDS             complement(10014..10970)
                     /locus_tag="ACHBKA_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM9445194.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11074..11676
                     /locus_tag="ACHBKA_001505"
     CDS             11074..11676
                     /locus_tag="ACHBKA_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9445195.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11764..13263)
                     /locus_tag="ACHBKA_001506"
     CDS             complement(11764..13263)
                     /locus_tag="ACHBKA_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM9445196.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13279..13803)
                     /locus_tag="ACHBKA_001507"
     CDS             complement(13279..13803)
                     /locus_tag="ACHBKA_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM9445197.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14101..14850
                     /locus_tag="ACHBKA_001508"
     CDS             14101..14850
                     /locus_tag="ACHBKA_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9445198.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14841..15626
                     /locus_tag="ACHBKA_001509"
     CDS             14841..15626
                     /locus_tag="ACHBKA_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9445199.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15595..16311
                     /locus_tag="ACHBKA_001510"
     CDS             15595..16311
                     /locus_tag="ACHBKA_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9445200.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(16413..17432)
                     /locus_tag="ACHBKA_001511"
     CDS             complement(16413..17432)
                     /locus_tag="ACHBKA_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM9445201.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17451..18452)
                     /locus_tag="ACHBKA_001512"
     CDS             complement(17451..18452)
                     /locus_tag="ACHBKA_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM9445202.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         5550 a         3904 c         3256 g         5831 t
ORIGIN      
        1 aatgttgttt caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt
       61 tacttatttt tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt
      121 aaatagttat ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga
      181 caaaaagcat gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg
      241 caataaggca agattttata aactacggga agtaatttcc gtaaatctat tcttattctt
      301 ttaagctgac aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg
      361 cgattcctgt caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat
      421 cttcttcttt ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc
      481 cgcccgcttc ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga
      541 tggactgaag aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact
      601 tttcgatcac tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt
      661 cccacggcat atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg
      721 ttacgcctag tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat
      781 atccactgtc tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt
      841 ctatagcaaa tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt
      901 ctactccgcg tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata
      961 ccttttgggg acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat
     1021 gaacaagaag cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc
     1081 cgtgttgcgt atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac
     1141 tttctatcag ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc
     1201 gcaggcttct tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga
     1261 tagtcagagg tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat
     1321 catgatgcgt tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta
     1381 gttgctcaaa ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa
     1441 gtatccgatt tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac
     1501 aagtttgatg atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc
     1561 cgacttgtac atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac
     1621 tgtgacaaat ggcagccctg gatacagtaa tgcagtgagt aaaaaatcag gctgtataca
     1681 ccagaacagt ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa
     1741 cagccagata ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat
     1801 atcaattagc gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg
     1861 aatcagatac caagctccaa tctttcccac taataaatac caatcaactg caaaaataat
     1921 gacaaacagc tgaaacaacg taatgatgaa acacatccgt ttgattttcc ctttcctcca
     1981 acataccttt ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa
     2041 aaaataaatc agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc
     2101 tagaactaaa gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata
     2161 gtagttgagc ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga
     2221 ccgcttgtac tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc
     2281 ggtagcagcg acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg
     2341 tctgcaaata atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc
     2401 aatggtccca tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc
     2461 gaaggtttca gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca
     2521 ataatcgctg attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa
     2581 atgaaaaatg cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa
     2641 ttcagccgat tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc
     2701 gttactaaca tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg
     2761 taagcaatac aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac
     2821 gaattcctaa agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa
     2881 ttttcccatt gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc
     2941 gttttaaaag aacctgcgat catccataag accggataaa tcatgactag acctaatagc
     3001 aaaataaaac cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat
     3061 tcacacaacg acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat
     3121 caacagcatg atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg
     3181 gttatacaca taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat
     3241 aaatgcctgc gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat
     3301 gatcggcgaa agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc
     3361 gatctctgcc gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat
     3421 cattgccgaa ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg
     3481 atcgctaaac caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc
     3541 gtttttagaa aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact
     3601 aggcacataa tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata
     3661 agcaatcaat agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt
     3721 gactcggatg gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc
     3781 gatccatgag actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat
     3841 catcggtatc actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc
     3901 gaccacattg tcttgatcca agaaccgttt tagtttttct tttttcatgc cttccttctg
     3961 ctgttacttc ttcataaatg actttttcaa ctaaaagatt gtattggtct tgaatttccg
     4021 gattcaaagc actatcaaag acattcactt cgccgttgtc catatcggaa atcatttcta
     4081 aataatcaaa tgtcgcttgc aaagttgcat catctgcacg agcgagctgt tctcgaatat
     4141 tcgacatgat tggtacacca cgttctcctt ttaaaatttc attggcttct tcactgtttt
     4201 gaaagaaatc aataaactta gcagcttctt ctttatgggc tgagcttttt gccatactca
     4261 gcatttgcga agaacgtaaa gggataccag attctccatc tgcttttctg cgaggtggat
     4321 taatcaactg aatctccata tcttcaggag cccctgacaa tatttctgtc aactggttac
     4381 ttgatagaaa caccattgcc gcttctctat ccctaaccgc ctgaaaagac agatccgtcg
     4441 tacttgcagc tccaccacct ggttctggat gggccccttt gtctaccaga tttttacgca
     4501 gctggaaaaa atctgttaga tagtctggat tttcaaatcc caagctgtta gatagatctt
     4561 cccgattata aaaattcaat cctttctcaa cttgtggtat ttgaataata cagccagcta
     4621 taaaatcttc gaatcttgaa atagcataga ttcctaattc ttcagtgatt tgatagctga
     4681 tttgttcaaa ttcatcccat gtccaatttg aagctggttc agggatattt aattgttcaa
     4741 atatattttt attgtaagca atcgcaaacg aattcacccc gttagacagt ccaactaatt
     4801 gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc attagaaata tcaattatcc
     4861 cttgctccac atattcatct aatggttcaa tctgattgat gtattgagga aattgattgt
     4921 tcagttggaa aatgtcccag atatcatctg atgctgctag tgtacctaat tttgtgtaat
     4981 atccttcata atcataaaat tcataatcga tcgtcacatg gggatttttt tcttcataaa
     5041 gctcgattac ttttactgtt gcatcgtgtc ggacttgcga accccaccaa gccatgttca
     5101 gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa gaagaataac ataacactta
     5161 aagctaagat gattctcatt cttttcttca ttctctgcac ctctacttcc attgttattt
     5221 ttcaatatac tgtattttta tacagaacat atttattaaa cgatcttttt caaggggaat
     5281 aaaaaccaag acttgcctta tcgttctgcc taggatgaac aactcaatac tttcaagaaa
     5341 aattagatag tgctgattct tctttcatcg ctaaaaacac ttcttgcttc aatgatggga
     5401 ggataattac gcaaagaaaa aaggatatca gacaactgcc caatatcctt gacatagcta
     5461 aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac agaaaaataa ctagtaattc
     5521 tgtatgaaat taggtacaat atacacgtcg atgcacaagc attgactatg gcaacttgat
     5581 tggtcagttc ccgctgactt ttcaagcccc gacaatttag ctgctactaa attgtcgggt
     5641 gcctttttta gtttttattc gtttttcatt ctatagaaag cgcttcaata aatcaagaca
     5701 tttttttgtt agaatgctaa taataacaaa aggttttaac tcttcttatg tacattacag
     5761 gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca
     5821 aaagacgagg aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca
     5881 tgattctttt tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta
     5941 cttggtggcg tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct
     6001 tccgttggac cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt
     6061 actgcttctg cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac
     6121 ttttcatcta tttctgtata tttcgacgtt accgtactga taactggaat atcaactact
     6181 cgacaaactt cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa
     6241 ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca
     6301 cctacaccgg agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga
     6361 tgaggtgtaa aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca
     6421 atatccgtag taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct
     6481 tctaaaatga tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc
     6541 gtaaattgtt ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt
     6601 gatacgtctc gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg
     6661 catcttgcaa tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca
     6721 gcgcaggatt ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt
     6781 cccatgaatc atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt
     6841 cctttgtttc caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat
     6901 tgtatttgct agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa
     6961 tgatactagg aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt
     7021 caataaattg ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag
     7081 acagattctt caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg
     7141 ctggccaacg gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt
     7201 cctacttttc atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat
     7261 actcagcgtt ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta
     7321 tatcttcgtg tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga
     7381 tccatttagc tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt
     7441 tgtaccagtt ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg
     7501 gctccgtaat cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac
     7561 tcatcacatc ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag
     7621 ctgagaccat cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt
     7681 ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact
     7741 catcaatttc tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg
     7801 ttttaacatc tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc
     7861 tgctggttgt ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga
     7921 ctgcttgatt gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg
     7981 ctgcgatcat aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt
     8041 cgttgttctt cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc
     8101 acccaatcga gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga
     8161 cgaaaattca aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca
     8221 aggtatctac cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg
     8281 ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg
     8341 tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca
     8401 taatcagtga aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca
     8461 tctttccatt tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt
     8521 tcagtttcaa actgttctaa taatacactc ataataagat caatatatac ttgatgcacg
     8581 tttatcccct ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg
     8641 ctgctttcat tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt
     8701 ataataatag acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa
     8761 atatcaatgg ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa
     8821 tcaagagacg agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt
     8881 cgctcaccgt tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc
     8941 tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc
     9001 agcattggaa gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga
     9061 actggctgac gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac
     9121 aatcttcaac atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg
     9181 ggctttaaaa atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg
     9241 gttcttcaat ccagaattag ctggaggagc tctgttggat atcggtacgt atgctgtatc
     9301 tttcgctcgt ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt
     9361 tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc
     9421 tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa
     9481 cggttatgtg acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga
     9541 tggtacaaag gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa
     9601 gaatatggta cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga
     9661 tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt
     9721 tgtgaaaaag ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta
     9781 tttttcgttt ggctggactt attaccgcag aggaaagctt ttgaacaccg tttaagaaag
     9841 aggttgtgag cctaccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct
     9901 catgtttgtt gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag
     9961 aagaggttgt gaaatcagct ttttgacctg aatattagct gaattcacaa cctctatttt
    10021 tggatttttt gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa
    10081 aactggaaca attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc
    10141 cgatacaata cataaacttc ttcaatacgt gttaaaattt gtctgccatc atttgactct
    10201 gaaaatgttt ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt
    10261 tgctggattc tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc
    10321 atagagaagt aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct
    10381 ctggcatctt cttggctttc gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca
    10441 tcttctttat tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct
    10501 ttttcgatct gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt
    10561 gcaagcaaag caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc
    10621 caagacatgt tttcctccac taaataatga gtgaccaaaa ccgaatttac tactatccct
    10681 tcggttaatt tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca
    10741 aaaatagtat acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag
    10801 gctgcgattc gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat
    10861 aatgcaatga tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc
    10921 gaaaagaaga cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc
    10981 cttttttatt ttaattatac ttgaataatg aaaaactagc tatcagacaa aagtttaggt
    11041 atgatagtaa aaagaccaaa aaggagaatc tctatgcaat ttggccgttt tcgactaggt
    11101 atgcgtacga tcaaaagtgc cttagctgtt tttttatgta tcttgttttt tcatttgact
    11161 caacgaggaa tcccgatgat tgctgcatta tctgctgttt tttctttgcg tcaggacttg
    11221 actacaaccg tttcttttgg aaagtctagg atcatcggca atacattagg tggtacatta
    11281 gctatcattt atttttttgt caaaagctat tttcacaatg attttttggt agaattgatc
    11341 ttgcttccta ttctagttat tattgtgatc gtcgtgtcag atggtattaa caataattcc
    11401 ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa
    11461 tccgctttgt atgctatcca acgtgtacta gatactttta tagggacatt cattgcaatc
    11521 ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga aggaagaact atcagaagat
    11581 ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa atttacaaga gatagaagct
    11641 aagatccaaa aacaaaagaa aaatagcgag gactaatttt cttttgcaat aaaataaaaa
    11701 aatggagaga agaactttaa aaagtcttcc tccatttttg attgaacggt gaagtgtact
    11761 actttattct tccttcatcg agttttgttc tgcctctttt ggcgcttcat acatatttac
    11821 ttggctagta gtcccacgtg tcgacaaatt ctctacgatt tccttcaagt caagtcctgt
    11881 tgtcgctttt agtgtttctt gagtagaaga aagcaaattt gtggcataat ttgtcactcg
    11941 gttcgctcca ctggtttctc ctgaactgct tcctgtatcg acaacagaga ttttttcgat
    12001 atttcctagc ggctgtgctg cttcgcgcat caattgcggt agcatatcga taaccatact
    12061 caagacagct gcttcgccat attccttgaa ggcattggcg atcttttgtt ttgcttcggc
    12121 ttctgctttt ccttttgcta gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt
    12181 attggcttcc gcttctgcca gtgcttccac acggaatcgt tctgcttcgg cttcagtcac
    12241 ttctttgact ttttgagctt gtgcttcttg ttctttagcg taccgatcgg catctgcttt
    12301 tttcttcact tccgaatcat attgcttttc acgacgtgtg atttcttttt cttccagttc
    12361 gatttgtttt tggcgttcta cgattttaat ctgcatttcc tgttcgatta cttgttgttg
    12421 tgctcgggca ctttctaaat tgtaggcttg gtctgctttt gctttagcaa catcttgttc
    12481 ttgtttgtag gttgctaatt tcagttcttt ttcttttaat gattctgcga tctctgtttg
    12541 tcgctgtaat tcagctgctt gtgattcttt ttctgcttga gcttttttga tacgcgtttc
    12601 ttttagagct tccgcttctg ctatatctgc atctcttttg acttgggcga ttcttggttt
    12661 ccctaaagag tcaagataac cgtttttgtc tctgacctct ttgatagtga aagaaacgat
    12721 gatcaacccc atttttgcaa ggtctacaga agcgacttct tgtacgcttt gactgaactt
    12781 atcgcgattt tgatagattt cttctacagt catagaacca agaatcgagc gcaagtgtcc
    12841 ttctaatact tctcttgctt cattttccag ttcttctctt gtcttgccta aaaactgttc
    12901 tgctgctgta gcaatctctt ctacagatga accgattttg atgatcgaag taccatcaca
    12961 catcaccggt acgccttgtt cggtatacac ttcaggggtg gagacgtcca atttactcga
    13021 taatagactg atccgattcg aacgttgaaa aactggcaag acgaatgcgc cacctccacg
    13081 cacaattttg attttgttat tgctttcatc aatgtgcaca tttttgcttc ctaaataact
    13141 tccactgatg atcaacgctt catccggttt tgctgtctgg tacttcgcta caaagacgat
    13201 cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc
    13261 catttaacac acgctccttt aagcttttat catgctttca taaggcatga cataacagat
    13321 tctttccttg atctcaatga tcaatacttt cgttccccgc tcagctgtct gttcttgggg
    13381 gctgtaaaaa caagctggac gactaatgga accggtaaca cttttcaact gaatttctcc
    13441 catacctcgt acagggatcg gtgtgatcac aacagctact tgtccttcca gagttttttc
    13501 agaagacgac atcgttgatt cggcatgttt catcggcatc aatatataga aattaagcaa
    13561 aaataccaac actatcgata tgcctccact agcaataaat aaaaataaag atgattgatc
    13621 tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa gttaaccaag ggataataag
    13681 catcggatca agcgggccat caaaatcgaa tacatctcca aaaataatca ataggactgc
    13741 caatcctgca caaatcagca atgtatacca ataaatcgtc tcaagtgcga ttccccctat
    13801 catatacacg cctccttcca tttctacgtc tatcatacca atgtttgaaa attctgtata
    13861 gcttctttta ccgactcagt ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt
    13921 tgttaaattt ttgttttcat gttaaaattt tcacaataaa ttctttcgag gtagcctatg
    13981 tttccttttt ttaagaaaaa accattaaag ctccagaaca cgatcatcct tgtcgtttgt
    14041 ttctccacat tcgtttcttt gattgtttct gccatattga tcaggaattt tattctttcg
    14101 atgaaccgat ctagtgtgtc agataaaatc tctagcattg ctaaaatcgt tgccaatgat
    14161 aaactcatcc aaaaaaagct gcagactggt gacccgacac atgcgattca aggaataaca
    14221 aatgaaatca tgattgcatc gaatttagat tttatcgttg tcatggaccc tgaaggcatt
    14281 cgatattctc acgcagaccc aagttacatc ggtcttcctt tctcaagtca tgaagatgtg
    14341 aaaaaagtga ttggaggaca agaatttttc tctgaaaaag aaggaatgtt agggttcggc
    14401 ttccgctatt tcgtccctat ttattatgat caagagatta ttggtgcagt ttgtgttggc
    14461 ttaacaaaca acacgattga caatcaagtc aaagatggac aaaaaatgat tgttttttca
    14521 ttgattattg gacttagcgt aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa
    14581 aaagtactgc ttggacttga accacaagaa atagcagaac agttaagtga aaaaacgatt
    14641 atcgaaaatg aagtagatga aggaatctta gccatctctt ctgataaaac tatcctcttg
    14701 atcaatacac ctgcaaaaaa cattttctac caaacagagc agctattgga tttcgaagta
    14761 ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa agagacgatt gagcatcaaa
    14821 cgaccgttcg gactcaatcc atgtatttaa atgggataga aactatttct agcgttacac
    14881 ctctttttag caatgaccgt tttttaggag cagtcgcaac tttacgaaat cgatcagaag
    14941 taatgaaatt aatggaggag cttgggggga cgaaacaata tataaactcc atgagagctc
    15001 aaacccatga attcatgaat aaacttcatg tcatttctgg tttgattgat ttgaagaagt
    15061 atgaagaagt cacccatttt atccagcagc taaatcaaaa ttataaaaaa gagattggcc
    15121 aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt tttattaggc aaaataaacg
    15181 aagccaaaga acaatctgtt gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg
    15241 agatggatga ctccatccat tcacttttgc aagtgttagg gaacttactt gataatgcaa
    15301 aagaagccgt gctacaaaaa catgaaagaa acggacaggt ccggattcat ttaaactatg
    15361 acacagaagc tgaagtattc atcgtgaccg tctgcgatgg cggtgtcggg atatcagaag
    15421 aaatctcaga aaaaatattt acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg
    15481 gattgaatct catccattca atcgtcaaac gccacggtgg atttttagaa ttttccaaac
    15541 aaacaaatgg cggtacaatc gtctatctag aattaccaaa aaaggaagat caaaatgatg
    15601 acaaatgtcc tgataatcga agatgatcca atggttgcag aattgaatcg tacttttgtg
    15661 gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa
    15721 tatttcagac aaaaaaacgc agatctgctg ctgcttgatg tatacatgcc tggcgaaagt
    15781 ggtatccatt ttttatcttg gcttagacag caaggaaatg atatcccagt gattttgatc
    15841 actgctgcag atgatatcga aaccataaag aatgtgatcc gctacggtgc cttagattat
    15901 ttgatcaaac cgttcacttt gcaacgtttt cagctggcga tcgaaaagta tcttgctttc
    15961 tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat tgatcgatca agtattcaac
    16021 cacaccactc ccattcacga aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa
    16081 ctgactttaa aaaaaataag tgcatttatt ctaaaagaag atactatttt ttcaacaaaa
    16141 gaactggctc aaaaaagctc actgtcacgt atatccgtca agaaatattt gaatttttta
    16201 gtagaacaag atatattgaa cgaaagaatc aaatatttgg aaatcggcca gccgctaacc
    16261 tgttatgaat tgacaaagaa tgggcggaaa aagctcacag attactgttg agtcatcgaa
    16321 ttatcccata caaaatagtc caagtataca gaagtaacaa ctgtttactt ggactatttt
    16381 taaacatttt attgacgaat atccatgttt tcttatctta attgacgggc tttaggaaga
    16441 gatacctcat ttttcgtttt tccagtttct attatttcac gaaagttctc gaaacttgtc
    16501 gaaatcatat ttgataatgc ctcatctgta tttgaaccta catgcggggt tacaagaact
    16561 ctagggtaca tttctactaa ttgctggata gcagggtctg gaatcgtctc ccatggcttg
    16621 aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat cggttgcaaa accagctaaa
    16681 tgattgcttt tcaaagctct taaaatagct tcattatctt gaagctctcc acgggctgta
    16741 ttaatcaaga tgctaccttt tttcatttga gctaaaaatc cttcattgat catttgatcg
    16801 ttttcacctg ggatatgagg tacatgaata ctgacaatat cgcttttggc taataactct
    16861 tcaagcgagt ggaacgtcaa tactttttta gcagcttctg tctgatgaat atcgtagccg
    16921 atcactttgg ctcctagtcc cttaaacaaa acagcttccg tcaaccctat cctgcctgtt
    16981 cctatgatcc ctactgtgca atttctgatt tcacgactaa acatttgctc atccactcga
    17041 aaatctttga aagatgttcg gatcgtcgta tacgcagtat ttctcaaaag catcatggca
    17101 aaagtcaaag ataactctgc tatcgcattt ggcgaatacg atggtacacg ggctactgtc
    17161 ataccaaaat cagctgccgc ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa
    17221 acataacgaa tcccatattc ctgcattttt gcaagattgg tctcatctgc tatacaattt
    17281 cccctcaaca atacagcatc atggtccttt gctgacacga tattgtcatg tgtcaacagt
    17341 tcttcataaa gactgagatc ataatggtaa atattcaatt tttcaaaaaa atctatctcg
    17401 ttcggacgaa caccgtaaca tgcgattctc atactttctt cctccatttc ttatgaaaat
    17461 aaacctatgc catgaatcat ttgtaataca acgatccata caggtgtcag taccacagca
    17521 aaaatagttg acagtaagga tgcattggag gctaaaagtg cctccctatc aaaactgata
    17581 gcataggctg ctgccactgt tgcagctggt gtagccatca tgataacgat tgttgccagt
    17641 gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa ctgctagaat cacgatattc
    17701 aatgcaggta caaataccac tttgactaga ctgtaatacc atgaagtttt ttcagatgca
    17761 gcttttttga agcttacttc acctaatgtt gcgccgattg ctagccacgc caatggtgaa
    17821 cttaaaccag ctaaataatc catcggctga aatagccacg gtgcagtctg gtcaattcgc
    17881 aaaaacgcta cttggcttat ctctccttct gcacctgtaa ctgacacttg tggcagatac
    17941 ccttggaaca gccaaatgaa aaggccggca aaagtagcga taatgattgg attgacgaac
    18001 atcgctttga tatttttagc ttccatcttc aagccactca tttttatgta tccataagaa
    18061 tacaaaaaga ttcggtagcc aatattgaaa attgaggaat acatcacgcc gacaggacca
    18121 taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca
    18181 cgcaacgttg tttgcttatc ccctttatag cttaagaaaa aaggttttga aactagaata
    18241 agaataatat aaataactaa tccccaaatc aagacattga ttccttgcat cagtaattct
    18301 gtattgatat cttgcatgaa agctcgaaat gccagtgctg gcaaagccac tgttaagacg
    18361 actttcgata gaatttttcc aacagattca gaaaaaatcc ccttttttct acaaacaaac
    18421 cctaataata taataaaaac tgttgaagtg attgcactga caatcgacat atctgtcaaa
    18481 gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc taattatgtt catttattca
    18541 c
//
LOCUS       DAYYOA010000049        18334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000049 DAYYOA010000000
VERSION     DAYYOA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.049"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..565)
                     /locus_tag="ACHBKA_001513"
     CDS             complement(110..565)
                     /locus_tag="ACHBKA_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445203.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="ACHBKA_001514"
     CDS             complement(719..1927)
                     /locus_tag="ACHBKA_001514"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM9445204.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="ACHBKA_001515"
     CDS             complement(2039..2371)
                     /locus_tag="ACHBKA_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9445205.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="ACHBKA_001516"
     CDS             2519..3394
                     /locus_tag="ACHBKA_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9445206.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="ACHBKA_001517"
     CDS             complement(3476..4057)
                     /locus_tag="ACHBKA_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM9445207.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="ACHBKA_001518"
     CDS             complement(4063..5322)
                     /locus_tag="ACHBKA_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9445208.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="ACHBKA_001519"
     CDS             complement(5324..6370)
                     /locus_tag="ACHBKA_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445209.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="ACHBKA_001520"
     CDS             complement(6605..6880)
                     /locus_tag="ACHBKA_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM9445210.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACHBKA_001521"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACHBKA_001521"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM9445211.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACHBKA_001522"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACHBKA_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM9445212.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACHBKA_001523"
     CDS             complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACHBKA_001523"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM9445213.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="ACHBKA_001524"
     CDS             complement(10708..11343)
                     /locus_tag="ACHBKA_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM9445214.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="ACHBKA_001525"
     CDS             complement(11409..12812)
                     /locus_tag="ACHBKA_001525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM9445215.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="ACHBKA_001526"
     CDS             complement(12799..13830)
                     /locus_tag="ACHBKA_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445216.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="ACHBKA_001527"
     CDS             13861..14082
                     /locus_tag="ACHBKA_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM9445217.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="ACHBKA_001528"
     CDS             complement(14247..14984)
                     /locus_tag="ACHBKA_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM9445218.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="ACHBKA_001529"
     CDS             complement(15137..16477)
                     /locus_tag="ACHBKA_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9445219.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="ACHBKA_001530"
     CDS             complement(16714..18237)
                     /locus_tag="ACHBKA_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9445220.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5173 a         3908 c         3101 g         6152 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca tttt
//
LOCUS       DAYYOA010000050        17976 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000050 DAYYOA010000000
VERSION     DAYYOA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.050"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1540)
                     /locus_tag="ACHBKA_001531"
     CDS             complement(62..1540)
                     /locus_tag="ACHBKA_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445221.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1838..2773)
                     /gene="murB"
                     /locus_tag="ACHBKA_001532"
     CDS             complement(1838..2773)
                     /gene="murB"
                     /locus_tag="ACHBKA_001532"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM9445222.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2846..3766)
                     /locus_tag="ACHBKA_001533"
     CDS             complement(2846..3766)
                     /locus_tag="ACHBKA_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9445223.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3879..4631
                     /locus_tag="ACHBKA_001534"
     CDS             3879..4631
                     /locus_tag="ACHBKA_001534"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM9445224.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5495..6028
                     /locus_tag="ACHBKA_001535"
     CDS             5495..6028
                     /locus_tag="ACHBKA_001535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9445225.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6518..7042)
                     /locus_tag="ACHBKA_001536"
     CDS             complement(6518..7042)
                     /locus_tag="ACHBKA_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM9445226.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7035..7508)
                     /gene="tsaE"
                     /locus_tag="ACHBKA_001537"
     CDS             complement(7035..7508)
                     /gene="tsaE"
                     /locus_tag="ACHBKA_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM9445227.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7631..8611)
                     /gene="pta"
                     /locus_tag="ACHBKA_001538"
     CDS             complement(7631..8611)
                     /gene="pta"
                     /locus_tag="ACHBKA_001538"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM9445228.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8764..9444)
                     /locus_tag="ACHBKA_001539"
     CDS             complement(8764..9444)
                     /locus_tag="ACHBKA_001539"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9445229.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9570..10439
                     /locus_tag="ACHBKA_001540"
     CDS             9570..10439
                     /locus_tag="ACHBKA_001540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9445230.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10465..10980
                     /locus_tag="ACHBKA_001541"
     CDS             10465..10980
                     /locus_tag="ACHBKA_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM9445231.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11027..11590)
                     /locus_tag="ACHBKA_001542"
     CDS             complement(11027..11590)
                     /locus_tag="ACHBKA_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445232.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11645..11824)
                     /locus_tag="ACHBKA_001543"
     CDS             complement(11645..11824)
                     /locus_tag="ACHBKA_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445233.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11901..12410)
                     /locus_tag="ACHBKA_001544"
     CDS             complement(11901..12410)
                     /locus_tag="ACHBKA_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445234.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12506..12886)
                     /locus_tag="ACHBKA_001545"
     CDS             complement(12506..12886)
                     /locus_tag="ACHBKA_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9445235.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13216..13965
                     /gene="pnuC"
                     /locus_tag="ACHBKA_001546"
     CDS             13216..13965
                     /gene="pnuC"
                     /locus_tag="ACHBKA_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM9445236.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14149..15426)
                     /locus_tag="ACHBKA_001547"
     CDS             complement(14149..15426)
                     /locus_tag="ACHBKA_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445237.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15505..16149)
                     /gene="pcp"
                     /locus_tag="ACHBKA_001548"
     CDS             complement(15505..16149)
                     /gene="pcp"
                     /locus_tag="ACHBKA_001548"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM9445238.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16348..17292)
                     /locus_tag="ACHBKA_001549"
     CDS             complement(16348..17292)
                     /locus_tag="ACHBKA_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM9445239.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17294..>17976)
                     /locus_tag="ACHBKA_001550"
     CDS             complement(17294..>17976)
                     /locus_tag="ACHBKA_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM9445240.1"
                     /translation="NYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGML
                     ELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFR
                     MIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFF
                     GQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAK
                     KYYPGKGQ"
BASE COUNT         5226 a         3676 c         3117 g         5957 t
ORIGIN      
        1 tctttgaaaa aaacataaat cagctaataa atatgtgaga caaagctgcc atccataaat
       61 actagctgat tttttcagtt aaatgtctat ttttttctct gatgatggta tttccaaaat
      121 attcccagtc atctggagtc ggtggatttt tccaaattat ctgttcttta ttaaattttt
      181 caagtaaaca atcagataaa taaatattcg tacgagaagt gattcgactc tcaatcacaa
      241 tattgtaatt tttaaatcct ttcacttgag aaataatgta atcggtataa gcagatcctt
      301 gcgaaaaatc aatacagaca tgaatcggtg cttcaaaata tgataccgga caaataccga
      361 ccaataaaaa gagataatca taaaataagc gaatcatgaa acgttcatct tttcccaaga
      421 aatagcgttt attctcttgt atgacctttt ggattgctgt actgaatatt ggatacgtct
      481 cgaagaaaaa ctgtagctgc tttttagacg taaagctagt catctcaaag tcgaatatcc
      541 aattttttcg attgacatgc atcataccca tacgaagttc ttttcgtata tatagtcttt
      601 cttcttcaga gatttcaatc gaaaatggta attgatcaat caattccact gacatttttt
      661 gactaatcat gtggatatca tgaaaatgag cttccgcaag tccaaatgcg ccatctaatt
      721 caaattcttc ttccgtgatc aaaaacaaca tgatataaga aatctcatca atgatctgct
      781 ctttagggat atgaagaaaa gaaatatgtg ataaatcctt gatgatcttc attccttctg
      841 agctcttaaa gtattcatct gaaaagaaac gttttccgat tggttgattc ccataggccc
      901 tcaacaaact aatagtggtt agcaaattga atttgattcg ttttgttgca gacagtttta
      961 aattgaacag atattcaaaa tattgcacga tactctttgc ttcattcatg acgctttctc
     1021 taaatggcat agaaaaacca ttaaagaccc cgaaatatac cccaaataat acgttccgta
     1081 tgtttatttc ttccccaact aatatgtttt tctttaactt tatttgatgc tgttcaagaa
     1141 aagagactaa ttctcttctt tttacatagg ctcttgatat gcttaaaaaa tgattctctg
     1201 caaatttttc gatcgttact tttgaatcca cgatctccgt taacagtcga gcaatttttg
     1261 atcgttgaaa gtagattctt cttaaatttt tgatatccgt caaattcaga cgttcgacat
     1321 ggatctctgc ttcttctgaa atttggatct ctggctttgt tttgaattca gtaaattctt
     1381 tatttaattc tatgatataa tttcttgttt taaatttaga caaacctaat gtttcatata
     1441 acttaggcag cactacataa ttctttttct caaattctaa atatctagct agcagcagtt
     1501 tttctctatc ttcctcttct ataaggtaat ccaacttcat atcctacacc tcaaaaaatg
     1561 cctcatacca attacttgat atgcagatga aagttcatct tttcacctat actgatttta
     1621 tgatggatta tgcttgtttt ttcatcagtt attctcattt tttatctttt ttttgagaat
     1681 attgcaaaaa atggaaaaat ttatgaggaa atattctata ttttttgttt tattgtgtta
     1741 aaatgtacaa gatttgcgcc atttgcgtaa acaaccacaa aaaagagcag gacggccacc
     1801 gtcctgctct tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg
     1861 tttctgatat tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa
     1921 atttttcttt gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg
     1981 catgatcgat attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt
     2041 tcaggccttg caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct
     2101 taaagacact tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca
     2161 actcatccat tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt
     2221 ccaatacgat agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt
     2281 cttttgtcaa agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat
     2341 ctttgatctc tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg
     2401 ggatgccgca agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat
     2461 caatcagtga ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca
     2521 tttcagtcag catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg
     2581 catttcctaa taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta
     2641 cttcttcttg ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg
     2701 tatagttcat caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt
     2761 cttctttatt cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg
     2821 cttaatacaa tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt
     2881 cctcacataa ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt
     2941 tacctaaacg gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta
     3001 gcgtgtttgt ccagtcgcca aattctttca ctttttttcc atcagcctct tgaatgacaa
     3061 gctgggtcag atcgtttact tcataagtac catttttact tgttacttga tatttttcta
     3121 tattagcgcc acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt
     3181 gtaaaatggc atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa
     3241 tgggttcatc taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga
     3301 cgaactctgt cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat
     3361 ctgggatacg tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca
     3421 aaattatttg gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac
     3481 tcagtggttt gtcaatataa acatggatac cattttctag acattttttg gctaattcat
     3541 aatgtacttt tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat
     3601 cgatcgtttc atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt
     3661 tatttcttgt tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat
     3721 atgctttttc tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg
     3781 gatcatttag ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg
     3841 caaaggtaag gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg
     3901 taaacgggct tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg
     3961 ccgatttttt ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc
     4021 caggttatta tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt
     4081 ttgctaaaaa accagcattg aatgccactt acggaatggg tatcgatatc catgatacag
     4141 aaggccgtct gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa
     4201 attctcaaag cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata
     4261 attatttgga aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg
     4321 ctcatcaaaa gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc
     4381 cagaagaaag agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt
     4441 atttttatcc aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga
     4501 aaaacaatgc tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac
     4561 tagctgatgc aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag
     4621 atttgaaata aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt
     4681 gaaaatggct ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa
     4741 cactgtttat cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
     4801 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt
     4861 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     4921 aaacaaaaat agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct
     4981 tttgaacacc gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca
     5041 gccaaacaaa aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa
     5101 gcttttgaac accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag
     5161 aaacaaatgg caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca
     5221 gctgattgaa caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa
     5281 gaaatttttt tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag
     5341 tcatgatccc catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa
     5401 caaatattac ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta
     5461 tgttacagta atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga
     5521 aactgccaat catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag
     5581 ccagatcgtt gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa
     5641 tgtccaaatt cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt
     5701 ctggcaaact atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc
     5761 ttttgataca aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta
     5821 tctttcttta tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg
     5881 attgaacact gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga
     5941 agatagtcgt gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc
     6001 attgaaaaaa cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt
     6061 gagcctgccg tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt
     6121 attaaatatt gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt
     6181 tgtgaaaaag ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
     6241 tttttcattt ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga
     6301 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc
     6361 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa
     6421 aaaaaatgga ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc
     6481 aaaaggatga cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt
     6541 tatatacatc taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga
     6601 tttggaagcc catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa
     6661 gctccaatcg aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa
     6721 gagtgcctag tcccattccc caatattcct gcagaatact gataccgact tctcccacgt
     6781 gagacaaacg aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat
     6841 agattgcaag cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta
     6901 aggtttctgg tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat
     6961 taacttgtcc catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga
     7021 tgatcccaac ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag
     7081 gtttcacttt gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca
     7141 cttttttcta aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc
     7201 cattcaacaa cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg
     7261 ctagctgcta cccgataaat atccatatga tacaacggaa tccgaccttg actatattct
     7321 ctaataatcg tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt
     7381 cctttcgtca acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct
     7441 ggcattgcgg aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt
     7501 tcgatcattt ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa
     7561 gtttttttaa agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg
     7621 cgtgtatttg ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat
     7681 cttcttcatt tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg
     7741 gtccgattgc ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact
     7801 gcaattctgg aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca
     7861 gttgtgcaac tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt
     7921 gtggcgctag ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt
     7981 tcgctgagcc ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt
     8041 ctgctgtttt tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt
     8101 tgatcgcaca atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg
     8161 ctccgcttgt ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag
     8221 tgtcgccagt cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa
     8281 gcatcgtacc aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct
     8341 ttccatttct acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag
     8401 ggctgatgat cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt
     8461 cttcttcgtt ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg
     8521 cgcctaaaat acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa
     8581 ttttaaatct taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta
     8641 ttatcataca ctattccaga aaaaaagaac agtttatttt atctgtatca gtcaaaaatg
     8701 ctggaaaaat taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt
     8761 ttattacact gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc
     8821 atcattcgtt ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact
     8881 gggatgtgga gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg
     8941 tgccggtttt ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat
     9001 gatcgtgtct gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg
     9061 aacggtaaga acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc
     9121 atgttgaaca ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga
     9181 tggcgggatc tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc
     9241 atgataagga tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa
     9301 tgcttcatag atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa
     9361 aaattctctt agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca
     9421 actgttatga atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt
     9481 ttcttcaatc acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga
     9541 acaagaagca gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg
     9601 gcacattatt agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg
     9661 caaatgatgc cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg
     9721 cagctttaga tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat
     9781 ttgataaaga aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg
     9841 ttttagatat tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt
     9901 attctgaaaa tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac
     9961 ctcaccttac gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca
    10021 tcacatacgt ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa
    10081 ttatttgttt tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc
    10141 ttttaggcga tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa
    10201 acgctcaaaa aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag
    10261 aagtcatgac gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca
    10321 gtttcgcaat gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca
    10381 caaaccttga atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt
    10441 tttatctaga aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga
    10501 cgtgcaactc ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt
    10561 cgctggggaa caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct
    10621 cacatcaagc agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa
    10681 aaggtgggaa ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga
    10741 cttcactgga cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat
    10801 cagcaaatca tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat
    10861 gtctcaaatg atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg
    10921 ctctaccgta aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa
    10981 aaagatgtac ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt
    11041 gcatatgatc ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc
    11101 gttcgtataa ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc
    11161 gattagcttt cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc
    11221 ctctaaaatc ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt
    11281 tatcaatgaa caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg
    11341 gctgatcgat gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac
    11401 cgtctatttc ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg
    11461 cttcttctaa aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca
    11521 tatcttttag aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa
    11581 aacgaatcat agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa
    11641 atcgttattt taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc
    11701 ttgccgcttc agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga
    11761 ctacattatt gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt
    11821 ccatgttatc tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa
    11881 agagaagcaa tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct
    11941 attaatcgga ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact
    12001 tttgcttttt tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac
    12061 tcttccgttt tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg
    12121 gttgttttcc cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat
    12181 tcatatgttg cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat
    12241 atgttatgtt cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga
    12301 tattcctggt cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg
    12361 gtaatcccta ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct
    12421 cctgtttctt ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga
    12481 aaaaaaagtg acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat
    12541 caaagaaaaa cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga
    12601 tccctaagga ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat
    12661 gacgcaagcc acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa
    12721 tctccaattc agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt
    12781 aattttctct gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat
    12841 aatctgaaac aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat
    12901 tcttcgttta agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga
    12961 aaaaaatagc tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc
    13021 tccaagacac tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag
    13081 aaaacaaaca gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt
    13141 caagaaaagc atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt
    13201 aaggagtcct catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg
    13261 ccgcaacaaa attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc
    13321 aatagtccca tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg
    13381 tgtattttat ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg
    13441 atttgtttta ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt
    13501 gcttatatga tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg
    13561 gcttcaaaaa tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc
    13621 cttgtctatc ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg
    13681 gtagatgcca ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac
    13741 aacaaccaat attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt
    13801 tcatttacgc atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg
    13861 aatgatgtcc ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg
    13921 caaaaaatgg aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg
    13981 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga
    14041 agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat
    14101 ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tctatttttt atagtaattc
    14161 cttcagatac tttcttgcct catcattgaa acgcagtgat tcttctaact tctgataata
    14221 ttccaacgct tgtttctgag cttcagtaaa acgataatgt tttcctttcc atttgtcgat
    14281 tgtctcttgc acatttccag attggatatc tttcactact agttgccatt tttcttttga
    14341 gactgtacgg tctttcttat cagcagattg tatagcttct cgttctttac gcaatacttt
    14401 taaaaaacca tttctgacta tccgaggaat gctgtcgaag aacccttctg taaaaccata
    14461 gcgacaatat tgcttccaaa taaatagata gatttttggt tggatgaatc gttggttttt
    14521 cgcaaaatat tccatctgag cagtgacttc tggtgttcgt tgacgttttg tctttctcaa
    14581 cacggaaata aatgtttcaa aataagcatt aggatgtact ggcacttctt catatacgat
    14641 ggattgccaa tctttacgta aaaatgcgcg atagttgatc aagaattctt ctacatcctt
    14701 tactaattct tctgcaaagt cgtcaaatag ttcttttttt atctgtcgct tttttgactg
    14761 cccattgatg tgttcactta ttttcttgat ttcattttcc aatgcttgat aatccgctat
    14821 gtaaatcggc atttctactt ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg
    14881 taaatatggc tgtttcatcc gacgattttg gtacaagaca aaacagtatt tttgccatag
    14941 tccttcagga aaatcgatat tcttctgctt aagccataat aattcgtcta aggccagatc
    15001 aacttttgtc attccctgat ggatcgaagc tgccactgtc ggagagacat gcaggtgatc
    15061 ggcaaagtgg ttgatgccta gttttaggat ctttccggtt tttggcgatt taacgatgta
    15121 ttgcactttt aattctctac cacactcaca atacaatttt tgctggcttc gttttgttcg
    15181 aaaaaacgga ttgatcttga agtcagaaaa cacccaatct gttgcagcta aataatgttc
    15241 ttccataaaa cgagagctga ttagatatcg gcggtgctta tcgactagtt cccgctgttt
    15301 tttcgttaat tttgcataga tctcccaacg ttgactgctt gtgtattgtt tagaaatttt
    15361 gatgggatag cttttgctga tttcttttgg aaaccattct tcaaagacgg attgccattc
    15421 ttccacggct gtttctttcc tttctaatta caaaaagctg gctactaaca taacgttagc
    15481 aaccagccag tgtttcttgc taaattagtg agttgttccg gcatttccgg aaatatcttc
    15541 tttattttct actgctgctt caactgcata agtcaaagaa gaagcaatgt cttctaaaga
    15601 catagaaggc gtacccactg gacgatcgat cacttgatcg ggtgagaatg gcacgtggat
    15661 gaacccacct cgaattgccg ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat
    15721 atcattgcag acaaatgttc ctgctgtata agagatgtac gctggtagac catgatcttg
    15781 tacatttttt gtcattgctt tgattggcaa tgaggtaaag taagcggctg gaccgttttc
    15841 ttctaaagcc gtatcgaatg gctgatttcc ttcattatca gggattcttg cttctgctag
    15901 attgattgcg acacgttcga acgagactgc tgaccgccca cctgcttgtc cgacacagat
    15961 cacgacatct ggttggtgct gttggatggc ttctttgact acttgactgc ttttttcgaa
    16021 tacggtcggt acttccactt tgatgatttc agcaccgctg atttcatctg gcattttttt
    16081 tactgcttca taggctggat tcactttgtc cccaccaaat ggatcaaaac ctgttactag
    16141 tactttcatc tgttgttcct ccgtccgatt gatttaacat atggttatct tttatcatac
    16201 cattttcctt gaacagcgta aataaaggaa acaatagttt aaataacata acagatcaaa
    16261 cttaatatat tcttataaaa aaatagtgtt ccactacaat gttagaagag gttgcgacaa
    16321 ttattctgtc acaacccctc tcttctatta tttaaataga atcatataag caatttggaa
    16381 aactagcata aataacgcaa taaacaactg gtttttgatc actccgtatt tgtctttcat
    16441 ctccagcatc gctacaggca cgatattgaa gttagcagcc atcggtgtca gcagggtccc
    16501 acaaaaaccg caagtcaatg caaccattcc tattaacgct ggattcgctc catgactaaa
    16561 tacgaatggt gcaccgatcc caaccgtcat cacggtgatg gccgcaaatg cattccccat
    16621 gattaccgta aatatcacca tgccaagagc gtaaatgatg atgccgatgt tcacatttcc
    16681 ttctggaacg atcgtaccta ctgcattgga aatcactgtc ccaactccag cgcttgtaaa
    16741 taccgcacct agagaagcta aaagcattgg caacatgctt aacggcccta ctgtgcccag
    16801 catatctgct gcatcatcta aaaatgtact tggtcgatta tctcgagaaa agaacatcaa
    16861 gattagcatg gctgcaaaaa caccgacgcc cacgccgact aaagcaccta aattggtaaa
    16921 tagagcaaac aagatcgcaa agacaccgat actcaaagct ggaataaaaa ttttcattcc
    16981 tagtttgtct gacatttttt ccatatattc ttttgatgga agccggcttt ctcctttgct
    17041 gactttctta gcaattgctg gtatggtcat cgcaaagatc aagccaccgt tcacgattgc
    17101 cggaagccat cttcctcctg caataactac ccctaagacc gtccagaaaa aggctgaccc
    17161 ataacgatga ggattggttt tatctgttaa atttcttact ccagtgtaaa tcaatacgag
    17221 tcccatcacg atataaagga tctccagtaa tttatcgcct aataaaatat caggatttgt
    17281 aaaaaagtcc atcttattgc ccctttcctg gataatattt cttcgctaat ttcttttctt
    17341 taagatagaa gtagacactt gccacaacta aagccgtgat agccactgga acttccacta
    17401 aagcaagctg tcccaatgtt acgtcatatc ctagatcttt cagtgttgat tggactagca
    17461 acgcccctgc tcctccgatg aatagtactt ggccaaagaa ccagcagaca ttttccatag
    17521 ctgaagccat accttttagt tcttcttcat gcttaggatg gacttctaac ccttttgatt
    17581 cgatggtccc caatgccatc ggtaaaatga ttggacgaac aaatccagca actccaccaa
    17641 aactcacgtt aaaagccgca aagatcattc gaaagatccc ataaatatct acaatcatac
    17701 cagcagatac tttcttgaat cgcttgatca aggaagccgc tgattctttc agaccatttc
    17761 tttcaagcgt tcctgttgcc aacataatga tgataaaaat cgccatcccg cgattgttta
    17821 caaaacttgt acctaaaagt tctaacattc cttgaatgcc aagaccgcca gtcaaagctg
    17881 taacaactaa agcaaccatt acgatcaata tcgagtctaa tttaaatgca aacccgagaa
    17941 taacaatcaa gacgcctaaa aggctaagat aatttt
//
LOCUS       DAYYOA010000051        17288 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000051 DAYYOA010000000
VERSION     DAYYOA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.051"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..1932
                     /locus_tag="ACHBKA_001551"
     CDS             166..1932
                     /locus_tag="ACHBKA_001551"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM9445241.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2034..3293
                     /locus_tag="ACHBKA_001552"
     CDS             2034..3293
                     /locus_tag="ACHBKA_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9445242.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3440..4741
                     /locus_tag="ACHBKA_001553"
     CDS             3440..4741
                     /locus_tag="ACHBKA_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445243.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4744..5595
                     /locus_tag="ACHBKA_001554"
     CDS             4744..5595
                     /locus_tag="ACHBKA_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9445244.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5608..6423
                     /locus_tag="ACHBKA_001555"
     CDS             5608..6423
                     /locus_tag="ACHBKA_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM9445245.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6574..7740
                     /locus_tag="ACHBKA_001556"
     CDS             6574..7740
                     /locus_tag="ACHBKA_001556"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9445246.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7970..8617
                     /locus_tag="ACHBKA_001557"
     CDS             7970..8617
                     /locus_tag="ACHBKA_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9445247.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8701..9645
                     /locus_tag="ACHBKA_001558"
     CDS             8701..9645
                     /locus_tag="ACHBKA_001558"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM9445248.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9780..11231
                     /locus_tag="ACHBKA_001559"
     CDS             9780..11231
                     /locus_tag="ACHBKA_001559"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM9445249.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11252..12088
                     /locus_tag="ACHBKA_001560"
     CDS             11252..12088
                     /locus_tag="ACHBKA_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9445250.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12088..12747
                     /locus_tag="ACHBKA_001561"
     CDS             12088..12747
                     /locus_tag="ACHBKA_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9445251.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12873..13562
                     /locus_tag="ACHBKA_001562"
     CDS             12873..13562
                     /locus_tag="ACHBKA_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445252.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13568..14524
                     /locus_tag="ACHBKA_001563"
     CDS             13568..14524
                     /locus_tag="ACHBKA_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM9445253.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14568..15080
                     /locus_tag="ACHBKA_001564"
     CDS             14568..15080
                     /locus_tag="ACHBKA_001564"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445254.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15336..15884)
                     /locus_tag="ACHBKA_001565"
     CDS             complement(15336..15884)
                     /locus_tag="ACHBKA_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9445255.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15973..17154)
                     /locus_tag="ACHBKA_001566"
     CDS             complement(15973..17154)
                     /locus_tag="ACHBKA_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM9445256.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5683 a         3081 c         3459 g         5065 t
ORIGIN      
        1 gtagactaaa acttttaaaa gttttaagtc tatctatttg gcaagtttaa aaaataaaaa
       61 ttcggtatca aaaaactgtt accgataaca tttttgaaag cgttgtcttt tgcttctcta
      121 tcacttatag tgatgacgtc atattaaaac acaaggagga aatcaatgga tacagctgca
      181 atttatcatc gaccggaaag tgaatatgca tttctatata cgaatacaag atttcgtatt
      241 cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg tgctatgtgg agatccatat
      301 accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg tctaagtacc
      361 aatgttcatg attattggga aattgaagtg acgagcgaaa caagtcgctt acagtatgcg
      421 ttccatgttg ttggggaaga taacacggac tgtttttacg gtgatcaagg aatttttcct
      481 tatcgagaaa atgtgattac tgaaccaaat ttttattttc gaatacctta ttttcatcaa
      541 atcgatcgat tcactattcc agaatgggta aaagaaacag tatggtatca aatttttccc
      601 gaaagatttg caaacgggga caaagcaaat gaccccgaac acactttacc ttggggaagt
      661 aaagatccag accgagaaga ctttttcggg ggagatttgc aaggtgtgat ggatcatttg
      721 gattacttga cggaccttgg catcaatggc atctattttt gtcccatatt taaagccaca
      781 tccaatcata aatacgatac gattgattat tatgaggtgg atcccgcttt tggcgataaa
      841 aaacttttaa aaaacttgat tgatgaagca cataaaagag ggatacgtat catgttcgat
      901 gctgtattca atcatatggg tgtccattca ccacagtggc aagacgttct caaaaacgga
      961 gaaaaatcga tctataaaga ctggttccac atccgttttt tcccagtaga ttcttatcag
     1021 atgactgact taccagaaac tgctgaaaat atcccatatg atacttttgc ttttactcca
     1081 ttcatgccga aactaaatac agcaaatcca gaagtgcaaa aatatttgct ggatatcgca
     1141 acttattgga taaaagaatt cgatattgat ggatggcgtc ttgatgttgc taatgaagtc
     1201 gatcatcatt tttggaaaaa attcagagaa gcagttacag aaatcaaacc agacatctat
     1261 atccttggag agatttggca ttcctctcaa gcttggctgc aaggggatga attccatgca
     1321 gtgatgaatt atgcatttac agattcgatt aaagattatt ttgcaaaaaa gaaaatcaca
     1381 gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc tttacagaga tcaggtgaac
     1441 gaaggaacat tcaacttatt agattctcat gatactgcca ggatcttgac cctttgccaa
     1501 ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa agggtctcct
     1561 tgtatctatt acgggacaga aatagggatg actggtgaag atgacccgga ttgccgcaaa
     1621 tgtatgatat gggagaagga ggaacaagat ctagagctat tcggttttat aaaagaactt
     1681 gtttctctaa ggaaacagtt gtctaagatt atttctgaag gttctactca gtggctaata
     1741 gtaaatgatc gtgaagataa gctctatttc acacggaaac tagagggaca gatcatctat
     1801 gtttacttta atcagtcaaa agaaccttgg gtagtagaac aggaaaatga ggtgattctt
     1861 agtcaaaatt gtcagttgtt agaagacggg aaagcagaga tacgacaata cggatttttg
     1921 atcatgtgtt gaaaactgtt accggtaaca acaagaaagc gtttgcaaat gaagatttgt
     1981 gcccattata ataagggtgt aacagcacgt tgaatacaaa ggaggaaatg aagatgaaaa
     2041 aaagaagtgt gaaacagttg ggtttagggt taatctcttt aggaatctta ggcggattag
     2101 cggcttgtgg caatggtaac tcttcagcag aaggggaagc cgatgaagga aaaacgttaa
     2161 cagtttccgt tgattctgga tataaagatt atataaacga aataaaaaac actttcgaaa
     2221 aagaaaacga tgtgaaaatc aagctagtag aaaaggacat gttcgatcag ttggaatcat
     2281 tagcgttaga tgggcctgct ggaaagggac ctgacgttat gatggcagca tatgaccgga
     2341 tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa actgggaaat gaagcagctt
     2401 acgatgaaac agacaaagcc caagtgactt atgatggaaa aatctacgga gaacctgcag
     2461 taatcgagac ccttgttttg tactataaca aagatttagt cgatacagca ccatcaacgt
     2521 ttaaagattt ggagaatcta tccaaagaca gtcgttttgc ttttgagtca gaagcaggaa
     2581 aaaatacagg atttttagct aaatggactg acttttatta ttcctatggt ttaattgcag
     2641 gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc ggtttgaata
     2701 atgcaggtgc tgtagaagga atctcttatg caacagattg gttcaaaaat gtttggccaa
     2761 agggaatgca agacataaaa agtgcaggag acttcgccag ccagcaattc atgtccaata
     2821 aaactgcagc aatcattgac ggtccatggc aagcgcaaac atacaaagaa aacaatatca
     2881 attatggtgt agccaaaatt cctactctaa ataatggaca gccttatcaa ccgtttggcg
     2941 gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa agacctctct caaaaatggc
     3001 ttgattatgt aacgaatcag gaaaatcaag aaaaattctt tgaaatggta aatgaaatcc
     3061 ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa cgatgaatta acaacagctg
     3121 tgattgaaca atatgaaaca gctcaagcga tgccaaacat cccagaaatg ggcgaagttt
     3181 ggacaggtgc agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca ccaaaagaat
     3241 ccgcagatga agcagtaaaa acaatcagtg aagccataga acaaaaatac tgattaaaat
     3301 gataaccgtc catttttata aaaaatggaa caggtgcttc agataaaagt ggcaagagcg
     3361 agatggaaaa ttatcttgct cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa
     3421 gtttagaggg ggcattcaga tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat
     3481 cacgctttgt tcacttattc cagggatggg gcagtttgta aatggccaag cttttaaagg
     3541 tatcgttttt ttcgccatat tcgtcctatt cattggtcag atgcttctgg gcggatttca
     3601 tgcattggta ggattggtta ctttgggaag tataccaatg gaagatcatt cattatttat
     3661 tttgattgaa ggcactttgc aattcatcat tacaatcatt ttcttgattt tttacggttt
     3721 gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg
     3781 gacgatgaag gaagtcatac ataatatagg tgatgaaggt tttccttatc tattgacact
     3841 tcccgcttat cttctcatga tttttacgat cattttccca gtcctagtta cattgttcac
     3901 tgcatttact aattatgact tcaatcatat tccaccagcc agtttaattg attgggtagg
     3961 ggctaaaaac ttttttagta tcttcttttt aagctcatat cgtaatactt tttttgcagt
     4021 cttttcgtgg accgtgattt ggactttatg tgcaacaact ttgcaaatca cccttggtat
     4081 tctcacagcg gttgttgctc accagaaatt tattaaattc aaacgtattt tcggtatcat
     4141 tttcttatta ccatgggcgg tcccagcttt tattactatc atgagttttt cgaatatatt
     4201 caacgatagc gtaggtgcca tcaatactca agtgattcct ttcataaacc agcttccttt
     4261 tgtagatatc agtgcaatct cttggaaaac ggacccgaac tggacgaaag tagcaattat
     4321 cttagtgcag ggctggttag gatttcccta tatctatgtg atggttacag gaatcttaca
     4381 atcgatttca gaagacttat atgaagcagc aaaaatcgac ggagcaaatg catggcagcg
     4441 cttttcaaac atcacgcttc ctgctgtctt cctagtggca gcgccaacat ttattacaca
     4501 gtatacgggg aatttcaata atttctccat gatctatctg tttaacaatg gcggtcccgg
     4561 aagcgtaggc ggaggagctg gttctactga tattcttatt tcctggatct ataaacttac
     4621 gactggaact tctccgcagt attctatggc ttcagcaatc acgttgatta tctccgtcat
     4681 cgttatctgt gtgtcattgc tggtatttaa aaagacaaat gcgttcaata tgggggatta
     4741 acaatgaaaa aatttcattc gagtgctcga ttccaaaaac gactttctca cttttttact
     4801 tacttttttc ttttaatact gtcagtcatt atcatctatc cgcttttgat cacagcaagt
     4861 actgccttca agtcaggaaa tattgcagct tttagtttag atctttcaag tgactggact
     4921 ttaggaaact ttactcggct attcagtgaa acactttacg ggacttggta taaaaataca
     4981 ttgatcattt ctgtcctaac aatgatcgta caagtgactg tagtaacttt ggcgggctat
     5041 acttatagtc gctatcgttt tatcggccgc aaatcaagtt tgatgttctt tttagtgatt
     5101 cagatggtac cgactatggc agcattgact gccttttatg tcatggcttt cttattagaa
     5161 gcgctcgata agtactggtt cttgacaatg atctatatag gcggtggaat tccaatgaat
     5221 acttggctga tgaaaggata ctttgacact gtaccaatag atttagatga atctgctaaa
     5281 cttgatgggg cagggcattt caggattttt ttccagattg tattaccgct tgtcaaaccg
     5341 atgctcgccg ttcaagcatt atgggctttt atgggacctt ttggggattt catgttagct
     5401 aagttcctgc ttcgaacacc tgaaaatcag actgttgctg tcggcttaca aacatttatt
     5461 tctgatgtga caaaccaaag agtctctctt tttgctgctg gggcaatttt gattgcacta
     5521 ccgatttgtc tattattctt ctttttacaa aagaactttg ttaccggcct gacatctggc
     5581 ggaacaaaag gataggagca gagtatcatg caaacatttc cgttaaacta tttttccgca
     5641 ttacgtacgc caactcaagt gtttgcggga cgcaaactat tatcatggcc taaatttttc
     5701 ttgatatttg tatttttagt ttccttgatg gtcatgcctg tgactctttt ttatgccaat
     5761 cagatccaag cgattccatt agaacaattt ctatccgttc attcgctgat cgatgaacaa
     5821 ggaacccaaa aattttctga acttgaatta tctgaaactg ggttacaagc atcgcaacaa
     5881 acgatagctg tgacccctga aattttggta ggtgtgtctt tatctgagaa acaacaatcc
     5941 gatcatggaa cttttattga ttttgagaag gaacaatggg tgatccaaca aaaggataaa
     6001 tctggtatac gccgctatac gatgaattat agcccatctt tccaaccaga ttctgtacgt
     6061 acgcccgaag atttccaacg atttttagaa agagaatttt atgccagtaa tcgtccaaca
     6121 atcattttat cttatagttt aagtcttggt cttgtcctat ttgtcatgac gagtctcatt
     6181 ttattcgggg cttccttttt cttgtggatg acaagaaaaa gccaactttc atccattcat
     6241 acattcaaag agagtgcaaa tttgatgctg aatgttatgg gaataggttc catggcagca
     6301 gcagttgtag ggtttatcca ctttgacttt atcttgatgc taagtgtcca aacaacgata
     6361 actgtcttgc tgcttttatg ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa
     6421 tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt
     6481 tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt cttttctttt ttagtattaa
     6541 cgtaataggg tagttttttt aaggaggaaa atcatgttag agaaaacttt atacaatcaa
     6601 ttattttcac gttctttctc gctccctgtc gaagtaaatt attgggacgg tacaacgaaa
     6661 caatacggag atacggacac tcctccccaa atcaaaatca atatccatga agaaatccca
     6721 atgaaagaga ttacaagtaa cgcatctttg gcactaggag aagcctatat ggatcatcga
     6781 atcgagatcg agggaagtat ccaagcattg atcaacgatg cttatcagca agcaaacagc
     6841 tttttacgag gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa
     6901 aaaaacaaag aagatatcca tgcgcattat gacttaggta acgactttta taaactttgg
     6961 ctagatccaa ctttgactta ttcttgtgct tatttcaata cacctgaaga tacgttggaa
     7021 caagcacaaa tcaataaagt ccatcatatc ttagacaaac tctttatcaa agaaggcgaa
     7081 acgttgcttg atatcggttg tggctgggga acattgatgt tcactgctac caaagagtat
     7141 aatgtcaagg caactgggat cacactaagt gaagaacaat acgatttgat taccaaaaga
     7201 ataaaagaag aacatttaga agataaatgc cgtgtattat taatggatta ccgcgaacta
     7261 aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa
     7321 aatcttgaag gttatttcaa agtggtaaaa gatttattga aaccaaaagg aacagctttg
     7381 atccatggaa tcagccgaca acaaggcggt gcgacaaatg cttggatcaa taaatatatt
     7441 ttccctggcg gttatgtccc tggtgttact gaattagttg ataatattac aaaaaacgat
     7501 ttgcaactga ttgatttgga aagcttacgt cgtgactacc aattgacatt agaacattgg
     7561 acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga ttccttctat
     7621 cgaatgtggg atctatattt acaagcttgt gcagcttcat ttgaatcaag taatatcgat
     7681 gtgattcaat atttgcttgt tcaacctgga aacaatgaca tcccaatgca tcgtgcataa
     7741 ttgaggcaaa agtactgttt aaagactatt taaaaagaag ctaagtttta tgatgctcca
     7801 gaagtgaact tttttgtcta acttctagga ttgtctctaa agcttagttt ctttttcttt
     7861 atattttcat aataaaagtt tggttcttta atttttaaac atgacagaaa attttttctt
     7921 cgttatactt gtaatagaat actattttat aacaaagggg atcgaatcaa tgaaaaaaac
     7981 tgttatttat actagtttat ttgcagcttt gtttttagct gcttgtcaat caaataccaa
     8041 tcaaggcaca tctgggaata cgtcttccag tagcgaacag cgctcttcac aggtatcgga
     8101 ctcctcaaca aaagcaagca catcttcgtc tagccagaca aaaagttcta gtgaagcaag
     8161 tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc gaattgttcc aagataaata
     8221 cccagatact gcaattacta gtttggaatt agatagtgat tggggatctt acttctacaa
     8281 gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa
     8341 aatggagaca gagaatccag aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa
     8401 agaagatggt ctggatgttg ataaattgat cacgattgag gaagcagcaa aaatcgccgt
     8461 cgacaaagta ggaagcggta cggcaacaga ttgggatttg gataaagatt taggtactac
     8521 ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca
     8581 aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa tttcttcgga aaacaagagt
     8641 tgatttttct tttagtcatg atacactaga caaaataact tgtttgaagg agcgatttac
     8701 ttgagtttag aaacgacctt tgccaatcat atctttgcca atcctttgat gaatgcatct
     8761 ggtgtccact gcatgactac ccaggaatta gatgaactgg ctcattcaga agcaggagca
     8821 tttatcacta agagctgtac gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt
     8881 gatgttcctt tagggagtat caattctatg ggacttccaa acttaggttt ttcttattat
     8941 ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt
     9001 tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt
     9061 gggtttaatg ggatcactga attaaattta tcttgtccca atgtgcctgg aaaaccacaa
     9121 ctagcttacg attttgaggc cacttatgaa acactgaaag aagtattcag catcttttct
     9181 aagccgctag gaatcaaatt gcctccttat tttgattttg ctcattttga tcagatggct
     9241 gatatcttga atcagtttcc attgacctat gtcaatgcta tcaacagtgt gggcaatggt
     9301 ttgtatatcg atacagaaca agaagcagta gtcatcaaac caaaagaagg gttcggcggc
     9361 attggcggag agtatatcaa gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt
     9421 ttgaaaccag aaatccaaat cattggtaca ggtgggattc ggacaggaca agatgcattt
     9481 gaacatctgc tatgtggcgc aagcatgtta cagattggta ccgagcttca taaagaaggg
     9541 ccggaaattt tttctagaat cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat
     9601 acgtcaattg acgaatttaa aggcaaatta cgcaccattt catagcaaag agacgaaggt
     9661 catgattgat cttcgtcttt ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg
     9721 tactcattgt atgttagacg aacttgtggg aaaataacag aggcaaaaag gaggataata
     9781 tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac
     9841 tagttacgga tatacagaaa aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg
     9901 cgcttgaacc attcgagagt aattcccacg agctattgat tccccagtca gaaaaagggc
     9961 cattggctgg tatcccattt cctttaaaaa tgctcggtca agaaaaaaag ggatggctag
    10021 ctacttcagg atctcgttta tttgaaacac accgagcttc tcatacatct aattatgtgc
    10081 ggcaagcaga agcgataggt ctagttcctt ttggacaaac caatgcgcca gagtttggtt
    10141 tcaaaaatat tacagaccca gtcatctatg gcccagcaag aaacccttgg aaccttgaat
    10201 gtacaccggg cggatcaagt ggaggagctg ctgctgcagt ttcatcagga atcgttcctt
    10261 tagctggtgc tagtgatggc ggtggttcta ttagaatacc cgcttcgttt tgcggattga
    10321 ttgggctgaa accttcgcga gggacgatgc cagttggtcc atatgcttgg agaggatggc
    10381 aaggagctgc tattgatttc ggcttgacag tttctatgag agacacggag gctttatttt
    10441 atgggatgag aagtatccat tccggtgcgc catatcaagt atcgccagtt gagtggcaaa
    10501 cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt tgattcaccg atccactcac
    10561 agattagtga agaggcagta cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag
    10621 ggcatgattt gacagagatt ccttacccac tagatggtcg taaactgatc acgtcttatt
    10681 atcagatgaa tggcgcagaa acagcagcaa tgatccgctc gattgaaaca ggcatccagc
    10741 gagctgtcag aaaagaagaa cttgaaccca tttcttggac tttgttccaa tatggtcaga
    10801 agctctctgc cgctacttac atcgattctc tgcatgtatg ggatgaagca gcaattgtca
    10861 tggaaaacct acatcaagaa tacgatctat tcctttcacc aacgactgca tttaccgccc
    10921 ctaaaatcag cgaagatttg caaagtgatg cgataagagc acagatggaa cacatcagtg
    10981 attgttctga agtagaaggt ttagacgttg tttcttccat gtttgaggag agcttgaaga
    11041 tcacacctta tacacagttg gctaatctga ctggacaacc agcaatcagc ttgcctactc
    11101 atttgacaaa agaaaacatg cctctaggaa tccaatttat ggctgccaga ggaagagaag
    11161 atttgctatt tcaaatcggc tatctatttg aagcaaatca tcagttccat ttaccaaatc
    11221 catggaaata gcagaaacaa ggagaagaag aatgaagaaa aaaagattgt ttgctctaat
    11281 aactagtgcc ttttttattt taagcgcttg cggtacagct gccgctaatc aggaagataa
    11341 cagctggagt aatgtagaaa aaaagaaaga attagtagta gcgacttctg gcacactgtt
    11401 tcccgcatct tattataatg atcaaaatga attagttggt tatgatgtag atttggcaaa
    11461 agaggtggct aaacggttaa atgtcaaaat ctcctttaaa gaatacaacg tggatggaca
    11521 aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg aacgattttg gtatcacaaa
    11581 agaaagagaa aaaaaattct ccctttcaga gactttcaaa tattcatttg acagtatgat
    11641 cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc
    11701 agctggcgaa cccaatacta gctatatgcg tttagcggaa aaacacggag ctaaattagt
    11761 cacatatgac aatgccacga atgatcagta tttaagcgat gtagcaaatg gtcgaacaga
    11821 tgttattcta aatgattatt atctgcagaa aatggccgta tctgctttac cagatatccc
    11881 tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa agtggttttt tattgaaaga
    11941 aggaaatgat gctctgaaaa agaaaatcga tggcgtatta aaagagatga aagaagatgg
    12001 gacgacaaag aaaatatctg aaaaatattt tcaaactgat gtttcagtca aaccgaaaga
    12061 aacgatggct gagtcggtga gtgagtgatg tactttcccc taattgattt ttccctcatg
    12121 tggacttcac ttcctttcgt actgcaaggc ttggtataca cattagggat tggcttcgtg
    12181 tcgtttcttt taggaaattt ggtagggttg cttttgacag ttttaggtct tttggattgg
    12241 ctgcctctga atgtatttat tcgtttttat ttatcgttct ttagaggcat tcccgcacta
    12301 gtcctgctgt ttctgttata tttcgggtta ccttatcaat taagtgcctt aacggcttca
    12361 gtcatctgct ttacgattac cagcagtgca tttattggtg aaatctatcg aggatcacta
    12421 gcaggcgttt cttcaggtca gtgggacgct ggtttagctt ctggtttttc tttctttggg
    12481 gtattgaaat atatcgtttt gccgcaagct tttcgaatct cagtaccggc actcagtaac
    12541 gtagctatgg atctagtgaa aggtacttcg ttagcagcaa caatcacagt gccagaaata
    12601 tttcaaaaag caaagatcat aggcggtcgg acattcgatt atttttctct ctatatttta
    12661 gttgcactga tttattgggt catttgtatc gggatcgaaa aagtgcaaca gtatttagaa
    12721 aaacgttcta tccatcagtc aatctgaaaa atagaaatgg tgctagcgtg gctggcacca
    12781 tttctatttt ttgtttcttg tatattctgc ctagtatttt aaaaccattt catgtaaaat
    12841 gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac cccgctaacc atcgaaatag
    12901 aatcctcgtt ataccaatat tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga
    12961 tgcctgatga ccaaggaatt gtagatacgc ttagctgctt gacaaaattt gatggaagcc
    13021 gagaatggcg atgctatgag ctgaaagtga caaaagcaga ttttcgctct acagccaaac
    13081 tgtcatttgt tggacattat aattattttg ttttaccaag taaactatat gaagaagtaa
    13141 aaactgaaat ccctacagga ataggtgttt tagtgtatcg tccgtacacg gttgaagagg
    13201 agatgcctgc ctctggtaca tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg
    13261 taccggaaga agctttgaca aaccgcttca tggcttcttt attcagggaa gtaagaaaag
    13321 caaagcaaat ggagcgaggc gtaaaatatt ttccaaaaga tcaattgtac aaagaattga
    13381 aaagacgttc agaaaatcaa gatccatttt ctaacgaacg atattatgaa cggtttttag
    13441 aggaaatagc tagtcaggca atcaatgagt tgcaagaaga agttatcgcg cttcagcatg
    13501 attatgaata tttgaggcaa caacagcaga tccgtcggtt gcctacagag ccattggagt
    13561 gaaaaatatg tactatcctt atttaagagg aaaacaattt gacctattag cattaaaaga
    13621 agcactgtcg cgaggattgc tctccaataa aatccaacca gtgatcgaac cagtacgtga
    13681 ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa aagcaccatc caatcgctgt
    13741 gatccaaaat ccccaagtag gacaattcaa attattcgat cagcatgtgc acagttggac
    13801 agtcaaagaa aatagctctg tcgtccctgc acagatctta acgccagaaa atctcagtga
    13861 agtactggat tcacctccag cgtttcttgt tttcgatgga cagcactacc caaaagatac
    13921 agaggtgtgg aaacagttgg caggagttga ttcgaaattt ttgataccag atactagtcg
    13981 ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa
    14041 gcatgttgaa agttatgcag ataaaacaga tgactttttt agtgatgact atcttttttt
    14101 ccacgcagac ggatatattg gattttctga tttcacgatc gaaggtagcc gttatttcga
    14161 taaagggttc cctagccgag cgcttgctat ccatctaacg tatattgatg cttacggcaa
    14221 tatacgcgtg aaacattttg tctctgacag taacgactct gctaaagacc aagcattgaa
    14281 gtttcttgaa gcaggagata aaatgaaaaa atggatcatg cgtcaccatc atcaactctt
    14341 gattacttca ggaatgattg aattattggc tctttaccag caacaaaaat tccctggact
    14401 gggagtgttg aaaaagtggt cacttatgca tcatttggaa cttattagtc acctgctaga
    14461 tcatccaaaa gattggctta aatattattc aaaagttcag gaactatatg aagtgatcca
    14521 atgattagat aatgtaaatg aaaaaggaat agaaaagagg aaactggatg tatattcggc
    14581 aaacaaggaa agaagaagta gaagaagtgg tagaaatcat taatgccgca gcggatttca
    14641 ttgcttcgca aggctctcct caatggcaaa acgggcagaa accaacaaaa gaaacgattt
    14701 tgaacgatat tgaaagaaaa gaaagctatg tattaatagt ggaagatcaa attgctggaa
    14761 cagctgcatt agtctcaggc gtggatcccg tttacacaaa aatagatggt agttggaaag
    14821 aatcaactgg tccttattta tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa
    14881 aaaaactagc aaaaccgttc ttagaacatt tgttgacgat tgcagccagc aaaaatatcc
    14941 aagatatccg tatcgatact tatccagcta atgaaccgat ggaaaagacc atctattctg
    15001 ctggattcac ttatcaaggg atgattgaat ttccttttga acacggcgaa agaaaagcat
    15061 atcaaataat catcaattga ctataaaatc atctgagcaa agataaagac attaacacta
    15121 ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa
    15181 acaagaaaga atttccgaaa atgcttctca aatttttgtg gaattttagc gtattttacg
    15241 aaatagctgc tttctgaaac tcccttcatc gagaggtcgt gacatagctt ttgtcacgat
    15301 ctctcggctt atcctacgag tattattcga tacatttata tcaataatcc tttttttatt
    15361 ttatctgcag cttcttggaa cgctccatcg tttgacacga ctaaaagtga ggggatagct
    15421 ttcaaacgtt tcaactgtgc taaatcttct acataaaccg ccagtctggc tgaaatctgt
    15481 tcagatgaat ctccacgttt tttcaggcgc gattgcaatg tttctttaga gatcgtaata
    15541 aagataggac ggactaccgt cccgaaatac tcatataaat gccaaaaacc atttgctgtc
    15601 aaaacggcgt aacaatttcc tactgtcagt ttctttttaa tttctgcttt actggaacca
    15661 tatttatgtt gagcatattg gtcccactct gcaaactcat ttcccttgat catctgattg
    15721 aatgtattgt cagaaacaaa ataataatcc tcttgatctt tttccccagg tcttggtgat
    15781 cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt
    15841 tttccagaac cagaaggacc gataaataca tagcaataat tcattgtcat ttctcctttg
    15901 atgattgaac aagtgaaaaa gctttggaaa atatttccaa agcttttaaa agtaactttg
    15961 cttttttgta ttttatacca gcaagccagt cacaatagca cttaaaaagc tgactaaagt
    16021 cgctgttagc aaaattttca atccgtgttt tgcgacaaga tttccttttt cttcatttaa
    16081 tcccttcatc gcaccagaaa tgatcccgat agaagagaag ttggcaaatg aaacgaggaa
    16141 gacagatata attgctgttg tacgctcagt aaaatggaaa ttaccatttg ctaattctgt
    16201 catagcaaca aattcatttg taaccagttt agtcgccatg atactaccgg catccactgc
    16261 ttctttccat gggattccgc tgatgaaggc aagaggggca aagatgtaac ctaggatctc
    16321 ttggaaagtg atgccgaata tcccgtggaa gatagcattg atcatggcaa tcaaagcaac
    16381 aaaaccgata agcatagcag caaccgtgat cgccacatgg aaaccatcaa gaatgtattc
    16441 tcccaacatt tggaaaaatg tctgttcttt gttttcttca atgatcagct catcttcttt
    16501 ttcttctaat acatagggat ttacaattga agccagaata taaccgccaa ataagttcaa
    16561 aacaagggca gtgatcacat atttagaatc gatcagtgcc atataagatc cgacgatcga
    16621 catagaaact gtagacatag ccgatacact catggtgaac aaacgtttct cagataggaa
    16681 gggtaattcc ttttttatgg aaataaaaac ttccgattgt cctaaaatag cagaagctac
    16741 tccattataa gattccaatt tgcccatccc gttgatttta cttaatccta gaccaacagc
    16801 tttcataaat aatggaagaa tcttgatata gcgtagaata ccgataatag cagaaataaa
    16861 tacaattggc atcaatacgg aaagaaagaa aggtgcactt cctttatttg ctacaccggc
    16921 aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa
    16981 tacagaaaca accgctgtac caaaagttgt acgtaataat acgaaaccaa gaacaagctg
    17041 taaaacgaac atcacaatta gtggtttcaa tttgattgct tttttgttac tgctgatcaa
    17101 aaaagcaagc cctaatgtga caaataaacc aataatacct aataaataac tcaaataatc
    17161 actcctattc caaattccgt tttattttac ctgttttgca aataaaatac taatctttag
    17221 tgaaattttc attacttttt tagagtgaaa cgcattcatt ttccattaga aaataaagcg
    17281 aacaaaat
//
LOCUS       DAYYOA010000052        17225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000052 DAYYOA010000000
VERSION     DAYYOA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.052"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACHBKA_001567"
     CDS             1..369
                     /locus_tag="ACHBKA_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM9445257.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACHBKA_001568"
     CDS             549..1490
                     /locus_tag="ACHBKA_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM9445258.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACHBKA_001569"
     CDS             1559..2290
                     /locus_tag="ACHBKA_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9445259.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACHBKA_001570"
     CDS             2434..4614
                     /locus_tag="ACHBKA_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM9445260.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACHBKA_001571"
     CDS             4634..5116
                     /locus_tag="ACHBKA_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM9445261.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACHBKA_001572"
     CDS             5132..5893
                     /locus_tag="ACHBKA_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9445262.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACHBKA_001573"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACHBKA_001573"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM9445263.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACHBKA_001574"
     CDS             7665..8150
                     /locus_tag="ACHBKA_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM9445264.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACHBKA_001575"
     CDS             8143..8826
                     /locus_tag="ACHBKA_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM9445265.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACHBKA_001576"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACHBKA_001576"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM9445266.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACHBKA_001577"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACHBKA_001577"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM9445267.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACHBKA_001578"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACHBKA_001578"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM9445268.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACHBKA_001579"
     CDS             13123..13455
                     /locus_tag="ACHBKA_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9445269.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHBKA_001580"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHBKA_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM9445270.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACHBKA_001581"
     CDS             14281..15654
                     /locus_tag="ACHBKA_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9445271.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHBKA_001582"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHBKA_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM9445272.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACHBKA_001583"
     CDS             16709..17149
                     /locus_tag="ACHBKA_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM9445273.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3009 c         3795 g         4546 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aatattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggga
//
LOCUS       DAYYOA010000053        16879 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000053 DAYYOA010000000
VERSION     DAYYOA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.053"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..641
                     /locus_tag="ACHBKA_001584"
     CDS             <1..641
                     /locus_tag="ACHBKA_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM9445274.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(712..2022)
                     /locus_tag="ACHBKA_001585"
     CDS             complement(712..2022)
                     /locus_tag="ACHBKA_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM9445275.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2034..3452)
                     /locus_tag="ACHBKA_001586"
     CDS             complement(2034..3452)
                     /locus_tag="ACHBKA_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9445276.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3692..5260)
                     /locus_tag="ACHBKA_001587"
     CDS             complement(3692..5260)
                     /locus_tag="ACHBKA_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM9445277.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5264..5545)
                     /locus_tag="ACHBKA_001588"
     CDS             complement(5264..5545)
                     /locus_tag="ACHBKA_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9445278.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5542..6465)
                     /locus_tag="ACHBKA_001589"
     CDS             complement(5542..6465)
                     /locus_tag="ACHBKA_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM9445279.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6462..7340)
                     /locus_tag="ACHBKA_001590"
     CDS             complement(6462..7340)
                     /locus_tag="ACHBKA_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM9445280.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7327..8664)
                     /locus_tag="ACHBKA_001591"
     CDS             complement(7327..8664)
                     /locus_tag="ACHBKA_001591"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM9445281.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8667..9770)
                     /locus_tag="ACHBKA_001592"
     CDS             complement(8667..9770)
                     /locus_tag="ACHBKA_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9445282.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9844..11352)
                     /locus_tag="ACHBKA_001593"
     CDS             complement(9844..11352)
                     /locus_tag="ACHBKA_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9445283.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11318..12010)
                     /locus_tag="ACHBKA_001594"
     CDS             complement(11318..12010)
                     /locus_tag="ACHBKA_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9445284.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12032..13006)
                     /locus_tag="ACHBKA_001595"
     CDS             complement(12032..13006)
                     /locus_tag="ACHBKA_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9445285.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13306..14178
                     /locus_tag="ACHBKA_001596"
     CDS             13306..14178
                     /locus_tag="ACHBKA_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445286.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14760..>16879)
                     /locus_tag="ACHBKA_001597"
     CDS             complement(14760..>16879)
                     /locus_tag="ACHBKA_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445287.1"
                     /translation="FIYQNTYESIESSYLTDKKNLLRQVKTNVEWKIRTIEYSFSTYG
                     STKNFSDIFKHPLTYTDYSTYSEIRKELNFIETIVMDDNNYDLVSLAGKWGVINGSLS
                     QLTEEEVEKYQQKYINNKNNLFWQKKNKGIEMVISLPMFEKEKYALGIATIEKHTIEQ
                     VVGESGEDILSIENEVGNLFTTQTVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLK
                     SDYNNWIYRLEVDSSAIGAMIRNLRIGLVAVSLTLVGLIGLFSYLFSDRFARPISQIQ
                     ERLNLKSKGFSGKELDLVAQSVSRIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVER
                     RELDQRLQQFGYSSQNDCYYTALVQIDLLEDSHGGERDLLLLAINNMITEIVPKENRM
                     LPIVLNEEMQATIYRMDKDDAEASKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLN
                     ESKQSVDRAKEALYYRVNTGPSSIIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGN
                     QEVKNLVHELIDALFAQNKNPLSREVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVK
                     ALNNYHPKELEKLVLEQLVLPITECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVI
                     ADRLHYNPNYLSNVFKKETGEKFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNP
                     QNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4986 a         3542 c         2757 g         5594 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagagaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata tatcattaaa
      661 aaaaataaag gtcaagctaa aatattattt aagcttgacc ttattataat ttcaacgatt
      721 gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata gataaatcac agcttttgaa
      781 acataagttt tgtactgctt cgattcgccc gaattctttt gcagttattt ggcaatgaga
      841 aaagcagaca tcttctatag gcttttcagg aaatgctact aaatcaaaag ctcgggaagg
      901 aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg atgtgtatgt ctttgacttt
      961 cgtcatttgc tttgattcag gtatttcttc agccactgct tgccaacttt ctggcatttg
     1021 agcaaggtca ttgctatttt ttctattgta ttcattatgc cagtccatta tccaagaaaa
     1081 aggaaattgt acatcaatca tattcagatt ttctaccctg atattttcga tcactccccc
     1141 tctttctttt gaagatttca ttcgaatacc acagtctgac tgaaaaaaat tgatatcgtg
     1201 aatatatacg tcactaactc cagcactaac ttcgctacca atagttacac cgtatcctga
     1261 acggatttta catctagcaa cctcgattct tgatgatttt cgattgactc ttgcaccatt
     1321 gccatctctg ccagacttaa tagcaataca atcatcacca caggccaaat cacattcata
     1381 gacacgcaca tttgttgaag aatcaatatc aatcccatct gtacttggtc catcattgtt
     1441 gcggatgatt actttttcta ctaatacatc gttagaataa gtgatttgca agttccaaaa
     1501 acctgatttc tgaaaaatca aatcacgaac aacaacatgt tctgcatgat acagtaaaca
     1561 agcgcgtgga cgtttaatag cataatccgc aatccaacga agtccctttt gatcgtattc
     1621 agctcttgtc ccgctttttt gatcttttcc ccaatacagc tcccaccaat ggtctccttg
     1681 accgtcgatt attccttttc cttctataag aatatctttc gccgagagaa cattcaaaat
     1741 cgctgctggc catttcattt ctacgccggc tactcgtgta tcgatttctg gaaatgcttc
     1801 gatatctgtt ctccccttga ggactgcacc ttcttcaaag ataagatgac tcttatcttt
     1861 taaaaaaagg gctccgacta aataaatgcc ttttggaata aatacaggta agtgttcttt
     1921 cgcaccttga tcgattgctt tttgcagtat tattgtatca tcttgttgag tgtcgtttgt
     1981 tttaaagttc aaagcattga taatcccttt ttttgtccgc aatacgtttc tccctatctt
     2041 tcattgattt ttttcaattc ttcaccaata aatacagttt ttgaacgatt cagtgggtaa
     2101 cctactagca aaatgattgc ggatacaaat aagcagaatg ctggtaacaa gtttgccatc
     2161 gtataaatcc tttcttgtac taaaactgat tggatggctc cacctgttgt agacgattgg
     2221 taaccaatga gtgccagcat aaatccgcct accgctcctg cacatgcttg tcctactttt
     2281 ctcgcaaaag aatttactcc atatactgtt ccatcttctc ttaagcctgt cttatattga
     2341 tgataatcaa tcacgtcagt aatgaatgcc cagaccatca aattgaataa accagatcct
     2401 aaagtagcga taaataaaag aatcagatat acccatgcat ttgttatatg aaggaaatac
     2461 atcagcaagt acatacatga agaagcaagt aacgcaaata cgcttgcttc ctttttacca
     2521 aataattttg tcagctttgt agcaaatggt gctaaaacta ctactgttcc gtaagtaaat
     2581 aaaagagcaa cagacattgc ttgtttatta tggaagtaat cattgaataa ataagtcata
     2641 ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt caacaatgac taaaataatc
     2701 agtgctcgat tagctaacaa accttttacc aactgagaag cagatacttt ttcgtttttg
     2761 acaactctta ctctttcagt agtcaattta caagttaaca tatatgcgac aaatgctatt
     2821 gcagcacatc cgattgaaat ccaaaagaaa tgattccctg aaacgacttg ttgtccactc
     2881 ttgttatggg tatacattaa aatcggaatg aagaatcctg tagttgatcc gccaatcgca
     2941 gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat taggatttga gctaattgct
     3001 gctgccattg ctccatacgg gatattgatt gttgagagaa aaatcccata aacaatgtat
     3061 gaaacaaaaa tataagcaat cttcaccgtc attggaagat gttggacgaa tggcaaaaac
     3121 aaaatgacag tcactaagca aaatggatac ttcatttttc tgatccaagg aataaatcgg
     3181 ccagtttctg ttaactctga gacatcacat aatcttccaa ccatcacatc agcaaacgca
     3241 tccaaaattc tcgaaatcaa gaataacgta cctacgattg cccctgaaac gccaagaaca
     3301 ttcgtataat aaatcatcaa gaaactattg actaaaccta aaataaaaca gttacctaga
     3361 tcaccgaaca tgtagcctat cttatcggaa atcccaaatt cctttttttc tttttttact
     3421 gtatttgctg taacagcatt ttgtgaaagc atttgaattc ctcctacctc ttctagtaaa
     3481 cgttttcaaa attataactc tttttttctt gtttttatat tcacaatgtt ggctttttat
     3541 tgcattatct tgacagataa aaaacaaaaa ataatctaaa aacattgatg cataagattg
     3601 ataacttttt ttacataaca atatatacaa acaaaaaaat tcactaacta gtgaaaataa
     3661 gttagtgaac ctcatttatt aaagaattaa attacagatg atcttttcca tcatacttga
     3721 aaaactcaaa atcagcataa cttcctcttt ttttatttag atcatgcact gctatcccga
     3781 taaaatttcc agtaaaccct cctgagagta tacacatgtt tttttgcgga cctactttct
     3841 tccattcttc agttgatacg tctttcaaat gaaactgtgc tgtctctttc tttacagtga
     3901 ctttcaaaaa agtcttcttt tctgtgagtg gaatcgtcca atcttctatt tgtgctactc
     3961 cgttttctga ttctatcata cgtaggcatt ttcccttgtc ctcattccaa gtaacataac
     4021 aataaatata attggattca tttaagtaca gtaccagtcc agccatctga ttaaaattat
     4081 taggcatgta ttcaaatgct gtctccgcat caaaagtaaa atccgtttgc cgaatagcta
     4141 acaaatgatg ttcgaaggta gattgaagag attcaccgga aatcagacgt aaaaaacctg
     4201 gtctacattt tgtatcacac caatcagcat ttggcaaaat gcgtctggca ttccagttct
     4261 tctttagctc tccttcaaat gtatccataa actgatgatc tttttcttgt tgtacctctt
     4321 catttgtctc aatcattatt tcttccatcg gagttgtctg gctatcttta agtcttaacc
     4381 aatcgtcgga cgtccaatac acttcttgaa tagcggtttc tcttcccaaa atagccgcac
     4441 cgtttaatgg tctggtacaa agatatgtca tgtaccatct tcctgacggg gtttgtacta
     4501 agctcccatg tcctgtacat tgcaatgtag aagcaggttt gtcactagct gtcagcatag
     4561 gaaaaccagg atcaagctcg taaggaccag taatattctt tgctcggcaa actgtaactg
     4621 aatgtcctct tcctgtaccg ccttctgccg tcatcaaata ataatacccg ttacgaaaat
     4681 acaaatgtgg tgcctccgtt ttcgctaatt ctgtacctgc aaatatttta tagatgggac
     4741 catcaagctt cttcttctct gcatcatatt cttgtaatac gataccgctg gatttattcg
     4801 gtgtgtccag acgataatcc catagttcat tgacaagcca tttttttccc gtttcaagat
     4861 catggaacaa agaaggatca aagccgttac tatttaaata aattggttcg gaccaaggac
     4921 ctgtgatctc agatgcagtg atcaaataat tgtggacatc tttaaacgga cggcctgcac
     4981 ttttgacatc tgtgtaaaca agataaaaca atccatcagc ataactgatc tgtggcgccc
     5041 atatacttcc atttgtagga tttccttgca aagatacttg agtggtcaaa atatccgttg
     5101 tatgtgacca gttgactaaa tttttcgact catatactcg gattcctgga agccattcaa
     5161 aagaggagac aataatgtaa taccgctctt tcactcttaa tatattagga tctggattga
     5221 atcctcttaa aataggattt ttcactttga tagtctgcat gattcaggct ccttttctca
     5281 ttcttactcg atataaactg aatgccccag ctgctaaaaa agcaagtgaa ataaacaaca
     5341 caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct aaagacaaag
     5401 ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca gacatattac
     5461 ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg accattccta
     5521 gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat gtatccattc
     5581 aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga cagtctgttc
     5641 atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac ctatcttaca
     5701 cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga cgtttctagt
     5761 atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt gatttttcgg
     5821 cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga tatgacactg
     5881 atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa tccagcaata
     5941 ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa aaggagtacc
     6001 atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac cagatagtcg
     6061 acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct gtcctacctt
     6121 ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat tcgatccttc
     6181 taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat actcctgttt
     6241 tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa aattggataa
     6301 tctgctttct atatattcat catatcttcc ttcttttact atcaagacct ttggatggct
     6361 atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat ccgtcggggt
     6421 atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg ctcccttatg
     6481 aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt taaagaaaaa
     6541 gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc ttttctcagc
     6601 ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct aagacgaggg
     6661 cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag atttggcaca
     6721 tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtgtttc gatgtccacc
     6781 tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac ccaccagctt
     6841 aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt gtcttccctt
     6901 gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca taacgttttt
     6961 cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg ttcttcaaat
     7021 ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc tgcaaaagat
     7081 ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga aaaatctccg
     7141 ccgtgagaat gcaggtaaag gaccgtcgga aacggtcctt ctttttctgg ataagcatag
     7201 agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag cataaaaccg
     7261 ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga tggagcacct
     7321 aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat ccatattttc
     7381 tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta atagaaatgc
     7441 tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg caaagccaat
     7501 ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa caccgggaac
     7561 tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta tgaattgttc
     7621 cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga tattcgaaaa
     7681 atggatcctt ctgaatgctg atgtccgttc atctatcgga taagcctttt tttcccatac
     7741 atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa atggacacat
     7801 cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc ctcgacctcg
     7861 tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg aaatggtaat
     7921 attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc catggaccat
     7981 cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat cttcagtccc
     8041 tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa tacgcacatt
     8101 tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt tcaagatcgt
     8161 caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac ttggtgactg
     8221 gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca tcaatttagg
     8281 acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt tttttccatt
     8341 tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag catagataca
     8401 ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag gataatcttt
     8461 tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga cattgctttt
     8521 taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc cgcctccgtc
     8581 acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat caattgatgc
     8641 gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa tgcttgcaag
     8701 aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta gtaagcatat
     8761 gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt attcaagttc
     8821 agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg tgatttcttc
     8881 acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt agccattgcg
     8941 taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt tgtttctgtt
     9001 gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc agccagtgtc
     9061 ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa ttcataggta
     9121 tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg ggatagacct
     9181 gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat cagcaaattt
     9241 gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac atcttggtaa
     9301 tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc catatataag
     9361 ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc ttcggatgtg
     9421 cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata tttttcttcg
     9481 cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc gatattgtat
     9541 tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc gatattgtct
     9601 tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg gaacccttta
     9661 agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc atataagcga
     9721 ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat tatttgctct
     9781 cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc ccaatcgtct
     9841 atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc gatcgcctcc
     9901 ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct gtcccatgat
     9961 gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt cattgttcgt
    10021 cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt ctttgatcaa
    10081 ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca cttcactagg
    10141 acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga taaattcgat
    10201 agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca tcaatttata
    10261 cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt gtgcttcgtc
    10321 ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt ctgaccaaac
    10381 atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt ccatatcatc
    10441 ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag tgtagattcc
    10501 agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg caaagtcttg
    10561 tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca tatactcttc
    10621 tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat ttggtgctcc
    10681 aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg cacgatcgcc
    10741 aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa attgtttagc
    10801 tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat ccaaccagtc
    10861 ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc cgtacaaggc
    10921 accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat ttttatagtc
    10981 ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc ggacagaaga
    11041 gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag aagccaatgc
    11101 agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca gttccgtatc
    11161 ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat gatgcaaaat
    11221 atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt tacttgaact
    11281 gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt ttacgagcgt
    11341 tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt tcttctgttt
    11401 tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct gtccacgtga
    11461 ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt gtttggatga
    11521 tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga agatgtgaga
    11581 atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca taatgagcca
    11641 tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata tacaaagcga
    11701 tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc ccgatggcgc
    11761 catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat gtgaagtacg
    11821 ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact gcaaccatcg
    11881 catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa ccgccccaca
    11941 gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt ttatctaatg
    12001 actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct cctagctttt
    12061 tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta aacaatccca
    12121 ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata taggtgtcga
    12181 aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc tcgaacccta
    12241 agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga cgaattgccg
    12301 gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct gcagcttcat
    12361 acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac cagcccaact
    12421 ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat gttagaaaac
    12481 tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg tcaacattca
    12541 aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg atgtaaatga
    12601 tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt aaagacaaaa
    12661 tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac gtatttctta
    12721 ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa ccgacccaag
    12781 gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg agtcccccca
    12841 taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc acatataaaa
    12901 atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt tcttcttttt
    12961 tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc ccctccttag
    13021 ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat tacgtatata
    13081 atcattttta tcgtaagtaa aaacgcttac atagcaatga tttttgtgct atacaaaacg
    13141 ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt ttttctatct
    13201 gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa attgaacata
    13261 tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa aaagaaagtc
    13321 tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt gctagctttg
    13381 atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac acaagaagtc
    13441 gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta tcgctacatt
    13501 ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac aggtgtcgga
    13561 acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag aaagtatttg
    13621 tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag cggggggatg
    13681 attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg ggccatgatc
    13741 ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt ccaagccttg
    13801 ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg tgtatttttc
    13861 aaaattatgc tgccattagc tttaccgtct attgctacta tctcgctttt ttatgctgtt
    13921 acttactgga acacttatat gactgctatt ttgtatatca acgattcatc taaatggccg
    13981 atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc agaaagtagt
    14041 gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt tattgcaaca
    14101 gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg agcactcgta
    14161 ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc agaagtggtc
    14221 agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg tgaaatttcg
    14281 gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt gaaagagacg
    14341 ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac ccacaggagc
    14401 aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc cttattgtcg
    14461 atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag acttttgtca
    14521 cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa atactagggt
    14581 tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc ttgattaaac
    14641 ggcgtttatc ttgttgattc tgcggcaata aaatcagcca gcagcaaaac atgaagtgct
    14701 gttttttgct gtcctctttt attgcttgaa tcagatcaca agaaaaacag cctcttgatt
    14761 cacaaatatt tttttcgata ttctcccggc gtaatcgatt ccttcttttt aaagaaacga
    14821 ataaaatttt gcggatttcg atattgcaat ctttctgcaa tctcttttac ggagaggtta
    14881 gtctctttca accatttttt tgctacctct aaacgatgat tttgaacaaa atcaccaaat
    14941 ttttctcctg tttccttttt aaatacatta cttaaatagt tagggttata atgcaaccta
    15001 tcagcgatca catctaaaga cagatcacga tcatattctg tgtagacgat atgcataata
    15061 ttctccgaca aatttttaaa ttcatgatca gtgattgctt gagagcattc tgtgattggc
    15121 agaactaatt gttctaaaac aagtttttct agttctttgg gatgataatt gttcagtgct
    15181 tttacataaa tttgtttcat gttatcaaag ttttttgaat ctactcccaa taactgtcct
    15241 aattgaacga gttcattgat caagcggacc gaaactactt cccgactcaa aggattttta
    15301 ttttgtgcaa acaatgcatc aatcaattca tgtactaaat tcttcacttc ctgatttcca
    15361 gaacgaatgg cttcaaacaa tcgattttgt tcttctatcg gatatctaat aagtgttttt
    15421 tcatgagcag caggaacgat ttcctgataa aaaataattg aacttggacc agtatttaca
    15481 cgataataca gggcttcttt tgcccgatca accgattgct tgctttcatt cagcgtttcg
    15541 aaccggttac tgatgcctat actgatagtt aaattcaagt attcttttac acgtttttga
    15601 atgagtcggc aatactccat gacttttttg cttgcttctg catcatcttt atccattcga
    15661 taaatagtgg cttgcatttc ttcattcaaa acgatcggca gcattcgatt ttcctttggc
    15721 acaatttccg tgatcatgtt gttgatcgct aaaagtagca aatcacgctc cccaccgtga
    15781 ctatcttcta acagatcgat ttgaaccaaa gctgtataat agcagtcatt ttgagaacta
    15841 taaccaaatt gctgcaaccg ctgatctagt tctcgccttt ctactcgatt tctaaataaa
    15901 ctaagtacaa atagcgtttc cagctgtggc ttttgggtaa ctaagtgtgc acttaacgct
    15961 tctttttctc ctatgatccg actgactgat tgagcgacca aatctagttc tttcccactg
    16021 aatccttttg atttaagatt cagacgttcc tgtatttgag aaatcggacg ggcaaatctg
    16081 tccgaaaata agtaggaaaa aagaccaatg agtcctacca gtgttaaaga cactgcaact
    16141 agcccgattc tcagattgcg gatcattgcg ccaattgcac tagagtcaac ttctaatctg
    16201 taaatccaat tattataatc tgatttcagt actatatacg ttttatcttt ttctcttatc
    16261 actttcagct gattgtaatc tagatccacg tccgttaata cagttggttg ctctttattt
    16321 tttactgtct gagtagtaaa aaggttgcca acttcgtttt caatagaaag tatgtcttct
    16381 ccactttcac caacaacttg ttctattgta tgtttctcta tcgtagcaat tcccaatgca
    16441 tatttttctt tttcaaacat tggcaaagaa atcaccattt cgattccttt gtttttcttt
    16501 tgccaaaata aattattttt gttatttatg tacttctgtt gatacttttc aacttcctct
    16561 tcggtcagtt gactcaacga tccattgatt actccccatt taccagccaa actgactaaa
    16621 tcataattat tatcatccat aacaatcgtt tctataaaat ttagttcttt cctgatttca
    16681 gaataagtgg agtaatctgt atatgtcagc ggatgcttga atatgtccga gaaatttttg
    16741 gtcgaaccat acgtagaaaa cgaatattca atcgttctaa ttttccattc aacgttagtt
    16801 ttaacttgcc tgagcaaatt ctttttgtct gttaaataac tcgattcgat cgattcatag
    16861 gtattctggt aaatgaata
//
LOCUS       DAYYOA010000054        16719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000054 DAYYOA010000000
VERSION     DAYYOA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.054"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..561)
                     /locus_tag="ACHBKA_001598"
     CDS             complement(<1..561)
                     /locus_tag="ACHBKA_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM9445288.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="ACHBKA_001599"
     CDS             complement(748..1398)
                     /locus_tag="ACHBKA_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445289.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ACHBKA_001600"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ACHBKA_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM9445290.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /gene="yvcK"
                     /locus_tag="ACHBKA_001601"
     CDS             complement(2910..3908)
                     /gene="yvcK"
                     /locus_tag="ACHBKA_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM9445291.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ACHBKA_001602"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ACHBKA_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM9445292.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="ACHBKA_001603"
     CDS             complement(4924..5655)
                     /locus_tag="ACHBKA_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM9445293.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="ACHBKA_001604"
     CDS             complement(5659..6924)
                     /locus_tag="ACHBKA_001604"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM9445294.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ACHBKA_001605"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ACHBKA_001605"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM9445295.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ACHBKA_001606"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ACHBKA_001606"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM9445296.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="ACHBKA_001607"
     CDS             12266..13768
                     /locus_tag="ACHBKA_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9445297.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="ACHBKA_001608"
     CDS             13770..14507
                     /locus_tag="ACHBKA_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9445298.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="ACHBKA_001609"
     CDS             complement(14552..14725)
                     /locus_tag="ACHBKA_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445299.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14737..15366
                     /locus_tag="ACHBKA_001610"
     CDS             14737..15366
                     /locus_tag="ACHBKA_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445300.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ACHBKA_001611"
     CDS             complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ACHBKA_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM9445301.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         4868 a         3627 c         2824 g         5400 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga catttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt
    15421 aatgacttag cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt
    15481 aaacttatcg aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc
    15541 gaatgcacgt aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac
    15601 tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa
    15661 acggaagttc gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac
    15721 agttggtact tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata
    15781 gtaaatttca gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata
    15841 caaatcacca aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg
    15901 ttgtccttct tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg
    15961 tcctgagcca tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac
    16021 atcacaagtt tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt
    16081 acctgcacca tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc
    16141 accacaagta tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat
    16201 cgcttcttca aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt
    16261 tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc
    16321 gaagccgcca ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc
    16381 gtaatttgga tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc
    16441 acttaaaatc tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt
    16501 attgatgtca ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc
    16561 tgatgctcct tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt
    16621 tcctcctatt ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa
    16681 acaaaaaccg atacttcaaa ctatttttca aaagaaaaa
//
LOCUS       DAYYOA010000055        16440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000055 DAYYOA010000000
VERSION     DAYYOA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.055"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACHBKA_001612"
     CDS             complement(29..727)
                     /locus_tag="ACHBKA_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM9445302.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACHBKA_001613"
     CDS             complement(720..2072)
                     /locus_tag="ACHBKA_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM9445303.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACHBKA_001614"
     CDS             complement(2295..3695)
                     /locus_tag="ACHBKA_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9445304.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACHBKA_001615"
     CDS             3856..4593
                     /locus_tag="ACHBKA_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM9445305.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACHBKA_001616"
     CDS             4718..5197
                     /locus_tag="ACHBKA_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM9445306.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACHBKA_001617"
     CDS             5194..5961
                     /locus_tag="ACHBKA_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM9445307.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACHBKA_001618"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACHBKA_001618"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9445308.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACHBKA_001619"
     CDS             complement(6864..7466)
                     /locus_tag="ACHBKA_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9445309.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACHBKA_001620"
     CDS             7572..7802
                     /locus_tag="ACHBKA_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445310.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACHBKA_001621"
     CDS             7793..8041
                     /locus_tag="ACHBKA_001621"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445311.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACHBKA_001622"
     CDS             8147..8731
                     /locus_tag="ACHBKA_001622"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9445312.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACHBKA_001623"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACHBKA_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM9445313.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACHBKA_001624"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACHBKA_001624"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM9445314.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACHBKA_001625"
     CDS             10632..10811
                     /locus_tag="ACHBKA_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445315.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACHBKA_001626"
     CDS             10833..11858
                     /locus_tag="ACHBKA_001626"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM9445316.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACHBKA_001627"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACHBKA_001627"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM9445317.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACHBKA_001628"
     CDS             13430..14377
                     /locus_tag="ACHBKA_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM9445318.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACHBKA_001629"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACHBKA_001629"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM9445319.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACHBKA_001630"
     CDS             complement(15279..15521)
                     /locus_tag="ACHBKA_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445320.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACHBKA_001631"
     tRNA            15642..15714
                     /locus_tag="ACHBKA_001631"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..>16440)
                     /locus_tag="ACHBKA_001632"
     CDS             complement(15833..>16440)
                     /locus_tag="ACHBKA_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HFM9445321.1"
                     /translation="KKGILGTIVVVLGLLGLLFAPSIISIFSLTITGFIAFTLVFGKK
                     DTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFII
                     LPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILL
                     IQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5607 a         2938 c         3312 g         4583 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
//
LOCUS       DAYYOA010000056        16375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000056 DAYYOA010000000
VERSION     DAYYOA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.056"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACHBKA_001633"
     CDS             135..2606
                     /locus_tag="ACHBKA_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9445322.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACHBKA_001634"
     CDS             complement(2709..4445)
                     /locus_tag="ACHBKA_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9445323.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACHBKA_001635"
     CDS             complement(4442..5146)
                     /locus_tag="ACHBKA_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9445324.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACHBKA_001636"
     CDS             5277..5780
                     /locus_tag="ACHBKA_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM9445325.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACHBKA_001637"
     CDS             5740..6078
                     /locus_tag="ACHBKA_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM9445326.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACHBKA_001638"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACHBKA_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM9445327.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACHBKA_001639"
     CDS             7629..8207
                     /locus_tag="ACHBKA_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM9445328.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACHBKA_001640"
     CDS             8211..8732
                     /locus_tag="ACHBKA_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM9445329.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACHBKA_001641"
     CDS             complement(8811..9365)
                     /locus_tag="ACHBKA_001641"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9445330.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHBKA_001642"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHBKA_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM9445331.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="ACHBKA_001643"
     CDS             9905..10156
                     /locus_tag="ACHBKA_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM9445332.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="ACHBKA_001644"
     CDS             10281..11072
                     /locus_tag="ACHBKA_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445333.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="ACHBKA_001645"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="ACHBKA_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM9445334.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="ACHBKA_001646"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="ACHBKA_001646"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM9445335.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="ACHBKA_001647"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="ACHBKA_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM9445336.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13129..14265)
                     /locus_tag="ACHBKA_001648"
     CDS             complement(13129..14265)
                     /locus_tag="ACHBKA_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9445337.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14381..15295)
                     /locus_tag="ACHBKA_001649"
     CDS             complement(14381..15295)
                     /locus_tag="ACHBKA_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM9445338.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15386..15814)
                     /locus_tag="ACHBKA_001650"
     CDS             complement(15386..15814)
                     /locus_tag="ACHBKA_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9445339.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15819..16193)
                     /locus_tag="ACHBKA_001651"
     CDS             complement(15819..16193)
                     /locus_tag="ACHBKA_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9445340.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5279 a         2952 c         3219 g         4925 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag actgagaaag
    13021 aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta acgatttcta
    13081 tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt acagtttttc
    13141 tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa tcttttcagt
    13201 gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag ttcctagttc
    13261 cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt
    13321 catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga aaggagttcc
    13381 tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct cattgtaaat
    13441 agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt ttgtagaatc
    13501 agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat ttataactac
    13561 tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg cttctccagg
    13621 cctcattcca gtcaaatata gccactggaa aaggagagaa tatcttttat tgtgtgaagt
    13681 tgtgaaattt actagtcttt tatattcatc atcttctaaa aacttatctt taatttttat
    13741 cgtttttgac tctcgtttat aatcaataac tacttcgttg ataggatttt tttctacata
    13801 gccttttttc atagcgtatg aaaaaagaag attcaatttg gatttaatta cactgacgta
    13861 cttatttgac aagtcatctt tatatatcat atcttcaaaa atattattta aatctactgt
    13921 attaatacca gaaacgatgt aagtctcagg tatctttttc tttatagtgt ttaagatatt
    13981 attcgtagga taatatgtag actccttaac ttgtcttttg taaataacta accattcttc
    14041 gacaagctca tgaaatgtta gatcaggctt ctgaagtgtt ttttcgttta attttatatc
    14101 aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt tatttttata
    14161 agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct cattatattt
    14221 atatactttt tttccgtttt tatcagtttt ggtttcaatc cacattgttt tcatctccta
    14281 tttgttgcta taataggaat ggataagtaa gccaattata gcaggtttat ttttcatcac
    14341 gtccacaaac tttggcgagg gagggggcgt gtttttttgt ttagtagcta cctttccatg
    14401 aagtatctgc gaagaacact tcaaatgttg ttccatcatt tttcacttca aaatatgctg
    14461 ttccttgtgc ggatttaccc ggttggatgg tttccgaaaa gtaatctcta ctagctaaat
    14521 cagatttaat atctgatgaa tcataaaatt ctaaataact tgaacttgca tcaaaaggtg
    14581 tagtccctgt gttttctaca agaaaatcta cctttgcgta aaataggcct tctggtttat
    14641 agaaatcatc accgtaatct tttgatacag actggacagt tacgttaata gagttaccat
    14701 tttcagaagt aaaaggaact gcttctccta tactatgtgc aactgatgcc gtctggggtt
    14761 ctgaagacga agttgtagtt gtagttgtag tagagcttga atctttggat tttccatctt
    14821 tatctaagaa agttcgttga ttataatata caacctgttc agtttttgga ctaacccaaa
    14881 ttaacattgt ttcatcatca gaatcagttg aataagtata ttggagcatc tctaattttt
    14941 ttatatcttt tgcactattt ccatgttcaa ttaattcaga gtgctcttta taataatctc
    15001 cttgtgcatc ctttgaaata gatacaatta attcatcccg tgtgatagca ttgtctaatt
    15061 ctttgtaaac aaaatcattt gtccatttac gtaacggtga accaagtgtc tgataaactt
    15121 gagttaaatc atcaccaact tttattttat gaacgtctga tgaagttagt ttttttgttt
    15181 catattcaga tgttgatgaa acagtatttt ctttagtgct agcagaatta ggactagtgg
    15241 agcatccgct taataccaag cctaaaaaaa gcaacccaaa aacaattttt ttcattttta
    15301 attcctcact tctatgatat gatttttttg taggatctta gaaataaggt ttttagtccg
    15361 tgttgcagca cggacttttt ttacattata taagaaagcg ataagcgctt tctggaagtc
    15421 cgtaaagatt ctttaattcc tcaattcgtt taggatattg atcattgtct tctttgtata
    15481 gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg cttctagatg
    15541 ttcttgttga tacatagtaa ctggataagc cacgatgaaa aatagcgtga cctaattcgt
    15601 gagcgcaaat gtagaatctt tcctcagagt ctctcagttc atcatttaag aagattattg
    15661 cacgacctct aatttcttga aactgtcctt tgggattctc gataaaagga acgtattgaa
    15721 ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat ttccgcttca
    15781 actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt gcctttttct
    15841 ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac gcgttggacc
    15901 tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc tagtaattta
    15961 tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct accgagtaaa
    16021 taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt actaggagtt
    16081 ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc caattgagtt
    16141 atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt cattttcgca
    16201 cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac aactaataaa
    16261 agtgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg ataactaata
    16321 aacacttcac ggtcggcaaa ctctgaaatg taaattacta ggcatttctg tgtct
//
LOCUS       DAYYOA010000057        16298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000057 DAYYOA010000000
VERSION     DAYYOA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.057"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(645..1148)
                     /locus_tag="ACHBKA_001652"
     CDS             complement(645..1148)
                     /locus_tag="ACHBKA_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9445341.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1507..2532
                     /locus_tag="ACHBKA_001653"
     CDS             1507..2532
                     /locus_tag="ACHBKA_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9445342.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2855..3733
                     /locus_tag="ACHBKA_001654"
     CDS             2855..3733
                     /locus_tag="ACHBKA_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM9445343.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4209..4325
                     /locus_tag="ACHBKA_001655"
     CDS             4209..4325
                     /locus_tag="ACHBKA_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9445344.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5253..5591
                     /locus_tag="ACHBKA_001656"
     CDS             5253..5591
                     /locus_tag="ACHBKA_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445345.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5685..5915
                     /locus_tag="ACHBKA_001657"
     CDS             5685..5915
                     /locus_tag="ACHBKA_001657"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM9445346.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6315..6686
                     /locus_tag="ACHBKA_001658"
     CDS             6315..6686
                     /locus_tag="ACHBKA_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM9445347.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6960..7652)
                     /locus_tag="ACHBKA_001659"
     CDS             complement(6960..7652)
                     /locus_tag="ACHBKA_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9445348.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7655..8440)
                     /locus_tag="ACHBKA_001660"
     CDS             complement(7655..8440)
                     /locus_tag="ACHBKA_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9445349.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8677..10077
                     /gene="lacG"
                     /locus_tag="ACHBKA_001661"
     CDS             8677..10077
                     /gene="lacG"
                     /locus_tag="ACHBKA_001661"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9445350.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10102..10419
                     /locus_tag="ACHBKA_001662"
     CDS             10102..10419
                     /locus_tag="ACHBKA_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9445351.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10432..12105
                     /locus_tag="ACHBKA_001663"
     CDS             10432..12105
                     /locus_tag="ACHBKA_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM9445352.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12119..13750
                     /locus_tag="ACHBKA_001664"
     CDS             12119..13750
                     /locus_tag="ACHBKA_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM9445353.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            14622..15812
                     /locus_tag="ACHBKA_001665"
     CDS             14622..15812
                     /locus_tag="ACHBKA_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFM9445354.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            15946..>16298
                     /locus_tag="ACHBKA_001666"
     CDS             15946..>16298
                     /locus_tag="ACHBKA_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9445355.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKG"
BASE COUNT         5218 a         2670 c         3315 g         5095 t
ORIGIN      
        1 ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa
       61 atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa
      121 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
      181 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      241 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      301 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      361 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      421 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      481 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      541 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      601 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      661 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      721 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      781 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      841 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      901 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      961 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
     1021 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
     1081 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1141 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1201 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1261 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1321 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1381 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1441 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1501 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1561 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1621 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1681 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1741 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1801 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1861 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1921 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1981 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     2041 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     2101 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2161 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2221 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2281 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2341 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2401 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2461 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2521 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2581 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2641 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2701 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2761 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2821 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2881 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2941 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     3001 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     3061 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3121 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3181 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3241 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3301 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3361 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3421 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3481 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3541 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3601 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3661 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3721 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3781 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3841 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3901 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3961 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     4021 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     4081 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4141 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4201 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4261 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4321 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4381 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4441 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4501 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4561 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4621 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4681 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4741 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4801 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4861 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4921 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4981 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     5041 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     5101 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5161 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5221 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5281 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5341 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5401 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5461 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5521 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5581 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5641 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5701 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5761 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5821 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5881 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5941 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
     6001 cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt aagtagctac
     6061 acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt cttaattagc
     6121 ttattgattc tataatattt ttgatttata taggaaatta aaaatattat agaatcaaca
     6181 ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa atttctcctg
     6241 gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac tttgcggagt
     6301 atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc atcaatcaat
     6361 cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc attatttctt
     6421 cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa
     6481 ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat cttaagttta
     6541 atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa gatacggaaa
     6601 cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact cagcttggtg
     6661 gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa gtatatcaaa
     6721 aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac actattttta
     6781 atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga
     6841 tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt tcactggcag
     6901 ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag ttgaatcgtt
     6961 taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa atctttgatg
     7021 gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc gaatggatca
     7081 attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct
     7141 tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt aagaaaaata
     7201 tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc ccagtcaaac
     7261 cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac agtagccgtg
     7321 gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc aagccctttt
     7381 gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct ttctttttct
     7441 tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc
     7501 tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa accatctttt
     7561 gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc caaaaacatt
     7621 gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg gtattttcgg
     7681 ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga
     7741 tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca taaaaatcat
     7801 ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta tttagagcga
     7861 tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg tgaacgccat
     7921 ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc
     7981 cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt tctggtccat
     8041 ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga attttcttgt
     8101 tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa atcgtttctc
     8161 cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga atctgcagag
     8221 tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt tgagcccctc
     8281 catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg cgaacggtca
     8341 tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct tttttattga
     8401 ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc tctaatttaa
     8461 ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag taaacattta
     8521 gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt ttatgattat
     8581 ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc agttagaaag
     8641 agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc agaagatttt
     8701 atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga aggtggcaaa
     8761 ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc cgatccggca
     8821 agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat
     8881 ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg gaaaccaaat
     8941 ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg
     9001 ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca tggagatttt
     9061 ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt tcatgaattc
     9121 aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac caatcaatat
     9181 ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt cgcctgtctt
     9241 cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg
     9301 ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga cgcgccggaa
     9361 gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt agatgctacc
     9421 tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg tgaagcaaat
     9481 caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt
     9541 aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta cgatggagaa
     9601 aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa agtgaaggga
     9661 atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg gctgatttat
     9721 ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca ttacaacaaa
     9781 atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg cattattatg
     9841 gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa ggcgataaca
     9901 gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt ctcatggaca
     9961 aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca gaagcgttac
    10021 ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa
    10081 gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg ttttgagatc
    10141 gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga agtaagacaa
    10201 ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt aacccttgca
    10261 cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat ggagatggga
    10321 ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag agatctgatt
    10381 caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc tatggatgga
    10441 atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg taatagttat
    10501 ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt ttcaagcatt
    10561 ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga atctgtctcc
    10621 acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact cgtttcagca
    10681 accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc
    10741 aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg
    10801 attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc ttcctttgtc
    10861 gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat taccattaaa
    10921 atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt tcctttctct
    10981 ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt ccaaatctca
    11041 tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga cgggtactta
    11101 ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg
    11161 tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac caatctgcaa
    11221 ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg gaattttgta
    11281 ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct actctttgct
    11341 aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag tttcgctgtc
    11401 aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt tattccattt
    11461 ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg
    11521 aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt ggttctagga
    11581 acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt tgacttcctg
    11641 atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga agcacaaaaa
    11701 gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga agtacctgtg
    11761 gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt actttgtgct
    11821 ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa
    11881 gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct gcaggatttt
    11941 gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa agaagacacc
    12001 gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg attaacgaga
    12061 gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag gagccataat
    12121 gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag gggctttggg
    12181 ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag cagatatttc
    12241 gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata tttcaaaaga
    12301 tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt tgtggcagga
    12361 tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg aaacgacgat
    12421 tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt ttcactacag
    12481 tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt
    12541 tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg aaatgaagca
    12601 gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg gaatgctctg
    12661 gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg ctgctttttt
    12721 gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag taatgctcca
    12781 cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa tcacgagtcg
    12841 aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg gttcaatgaa
    12901 tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt
    12961 tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg cttttggtga
    13021 agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag agtatttgct
    13081 ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct tcttctattg
    13141 ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta tgaattatct
    13201 cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg
    13261 aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa
    13321 aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc aaacctggtt
    13381 tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt ttggggacta
    13441 caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa atgttagtgg
    13501 cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag agtttgatac
    13561 taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg ttgttaaaac
    13621 aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg tttgtgaaat
    13681 tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg tttcattggt
    13741 gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca gccacatcgt
    13801 tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga
    13861 tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac tttgtattat
    13921 gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag
    13981 ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga
    14041 gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag aacttctttt
    14101 tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg gaaccatctt
    14161 atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata gggattcccc
    14221 ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg tataaaacag
    14281 aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac tataaataaa
    14341 aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt atatatgaaa
    14401 actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa cgtcttttat
    14461 atattacttt caaataaata tgaggtagaa tactatctat tttcttttta acccaattgt
    14521 tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc ttctgtagaa
    14581 tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat tttactacag
    14641 aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt gtccatttgg
    14701 agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat tacgaaaagt
    14761 atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac cgtacggtca
    14821 agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt gagttcaagc
    14881 aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc gtcattcacc
    14941 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
    15001 attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa gaggtaacgg
    15061 cgtttaatgg gcgggagctt catgaccgtt atgctgctat ttatatggac gcaacgtata
    15121 ttccgttaaa gcggaaaacc gtcgccaagg aagctattca tatcgcagtt ggcattcgcc
    15181 cggacggatc aaaggaagta ttgagctatg cgattgcacc gactgaatcc atcacgattt
    15241 gggaggaaat tttattggac cttcaagagc gcggtttgaa aaatgtcctc ctgttcatca
    15301 cggatggctt aaaggggatg gtaggagcga ttagtcggtt ctatcccaaa gctcgttttc
    15361 aacattgttg tgtacacgtt tcccgtaata tcagtcataa agtgcgtgtc gatgatcgta
    15421 aggaagtctg tgatgatttt aaaatggtgt atcaagcctc atctaaagag gtggcgttgg
    15481 aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta tccaaaagtg gttgaatcga
    15541 ttctttcgaa cgatcacttg ctcactttct atgatttccc cttggccata cgcaagagta
    15601 tttattctac gaacttgatt gaatccttta ataaacaaat caagaaatac agccaccgca
    15661 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
    15721 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
    15781 aacaaatgct aagccaactg attgagaatt agaaacaatc tgcagtaggg aagaatcatt
    15841 tacacaaaat tattgacgct cccttataat gagaactttt actaacataa tttatgataa
    15901 acttactata taatcatatt aattaaatag ttaggagatc tgcttatgtt tatatctact
    15961 cttcaaattc gaaattttag aaattatgaa tccgaaatct ttcgattcga taatgagaca
    16021 aatactatta taggtgagaa tgattcaggg aaaaccaatg ctcttactgc attgagaatc
    16081 ttattagatg atagttatta ctatagtagt aaaacactaa aagaatcaga tttttttcat
    16141 ggaattcaaa atgggtggca agggcattgg attattatct ctgcgacatt tgaaggaatc
    16201 agcgaagaag aatttgacaa tgagatttgt gcttccattt ctttaaattc tgaaagtcaa
    16261 acaatcttag aagaattgat ttcaaatgca gataaagg
//
LOCUS       DAYYOA010000058        16225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000058 DAYYOA010000000
VERSION     DAYYOA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.058"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACHBKA_001667"
     CDS             138..1475
                     /locus_tag="ACHBKA_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM9445356.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACHBKA_001668"
     CDS             1523..3652
                     /locus_tag="ACHBKA_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM9445357.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACHBKA_001669"
     CDS             3627..4316
                     /locus_tag="ACHBKA_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445358.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACHBKA_001670"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACHBKA_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACHBKA_001671"
     CDS             complement(5546..5740)
                     /locus_tag="ACHBKA_001671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445359.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACHBKA_001672"
     CDS             complement(5790..6230)
                     /locus_tag="ACHBKA_001672"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM9445360.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACHBKA_001673"
     CDS             complement(6234..7079)
                     /locus_tag="ACHBKA_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM9445361.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACHBKA_001674"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACHBKA_001674"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM9445362.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACHBKA_001675"
     CDS             complement(8703..9650)
                     /locus_tag="ACHBKA_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9445363.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACHBKA_001676"
     CDS             complement(9873..10223)
                     /locus_tag="ACHBKA_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9445364.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACHBKA_001677"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACHBKA_001677"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM9445365.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACHBKA_001678"
     CDS             11646..11966
                     /locus_tag="ACHBKA_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9445366.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACHBKA_001679"
     CDS             11988..12452
                     /locus_tag="ACHBKA_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9445367.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACHBKA_001680"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACHBKA_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM9445368.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACHBKA_001681"
     CDS             complement(13301..14590)
                     /locus_tag="ACHBKA_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM9445369.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACHBKA_001682"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACHBKA_001682"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM9445370.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15825
                     /locus_tag="ACHBKA_001683"
     CDS             15700..15825
                     /locus_tag="ACHBKA_001683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445371.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            15822..>16225
                     /locus_tag="ACHBKA_001684"
     CDS             15822..>16225
                     /locus_tag="ACHBKA_001684"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9445372.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIV"
BASE COUNT         5134 a         2971 c         3297 g         4823 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttga
//
LOCUS       DAYYOA010000059        15987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000059 DAYYOA010000000
VERSION     DAYYOA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.059"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHBKA_001685"
     CDS             226..894
                     /locus_tag="ACHBKA_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM9445373.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACHBKA_001686"
     CDS             complement(1094..1480)
                     /locus_tag="ACHBKA_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445374.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACHBKA_001687"
     CDS             complement(1452..2834)
                     /locus_tag="ACHBKA_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9445375.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACHBKA_001688"
     CDS             complement(2990..4177)
                     /locus_tag="ACHBKA_001688"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM9445376.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACHBKA_001689"
     CDS             complement(4184..5215)
                     /locus_tag="ACHBKA_001689"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9445377.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACHBKA_001690"
     CDS             complement(5231..6685)
                     /locus_tag="ACHBKA_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM9445378.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACHBKA_001691"
     CDS             6860..7765
                     /locus_tag="ACHBKA_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM9445379.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACHBKA_001692"
     CDS             7856..8533
                     /locus_tag="ACHBKA_001692"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9445380.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACHBKA_001693"
     CDS             8530..8700
                     /locus_tag="ACHBKA_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445381.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACHBKA_001694"
     CDS             8814..9710
                     /locus_tag="ACHBKA_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9445382.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACHBKA_001695"
     CDS             complement(9765..11135)
                     /locus_tag="ACHBKA_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9445383.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACHBKA_001696"
     CDS             11373..12119
                     /locus_tag="ACHBKA_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9445384.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACHBKA_001697"
     CDS             12116..12955
                     /locus_tag="ACHBKA_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9445385.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACHBKA_001698"
     CDS             12956..13906
                     /locus_tag="ACHBKA_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9445386.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACHBKA_001699"
     CDS             14047..14799
                     /locus_tag="ACHBKA_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445387.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ACHBKA_001700"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ACHBKA_001700"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM9445388.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5189 a         3001 c         3220 g         4577 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattat
//
LOCUS       DAYYOA010000060        15952 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000060 DAYYOA010000000
VERSION     DAYYOA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.060"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..809)
                     /locus_tag="ACHBKA_001701"
     CDS             complement(387..809)
                     /locus_tag="ACHBKA_001701"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM9445389.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="ACHBKA_001702"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="ACHBKA_001702"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM9445390.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="ACHBKA_001703"
     CDS             complement(2252..3154)
                     /locus_tag="ACHBKA_001703"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM9445391.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /gene="atpA"
                     /locus_tag="ACHBKA_001704"
     CDS             complement(3168..4724)
                     /gene="atpA"
                     /locus_tag="ACHBKA_001704"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM9445392.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="ACHBKA_001705"
     CDS             complement(4747..5289)
                     /locus_tag="ACHBKA_001705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM9445393.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="ACHBKA_001706"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="ACHBKA_001706"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM9445394.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="ACHBKA_001707"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="ACHBKA_001707"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM9445395.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="ACHBKA_001708"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="ACHBKA_001708"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM9445396.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..7644)
                     /gene="smpB"
                     /locus_tag="ACHBKA_001709"
     CDS             complement(7180..7644)
                     /gene="smpB"
                     /locus_tag="ACHBKA_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM9445397.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7666..10023)
                     /gene="rnr"
                     /locus_tag="ACHBKA_001710"
     CDS             complement(7666..10023)
                     /gene="rnr"
                     /locus_tag="ACHBKA_001710"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM9445398.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10026..10781)
                     /locus_tag="ACHBKA_001711"
     CDS             complement(10026..10781)
                     /locus_tag="ACHBKA_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9445399.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="ACHBKA_001712"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="ACHBKA_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM9445400.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="ACHBKA_001713"
     CDS             complement(11238..11516)
                     /locus_tag="ACHBKA_001713"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445401.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="ACHBKA_001714"
     CDS             complement(11629..12597)
                     /locus_tag="ACHBKA_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9445402.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="ACHBKA_001715"
     CDS             12880..13854
                     /locus_tag="ACHBKA_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM9445403.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="ACHBKA_001716"
     CDS             13859..14434
                     /locus_tag="ACHBKA_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM9445404.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="ACHBKA_001717"
     CDS             complement(14501..15640)
                     /locus_tag="ACHBKA_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM9445405.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5296 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgatat tt
//
LOCUS       DAYYOA010000061        15620 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000061 DAYYOA010000000
VERSION     DAYYOA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.061"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..618
                     /locus_tag="ACHBKA_001718"
     CDS             <1..618
                     /locus_tag="ACHBKA_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445406.1"
                     /translation="TLYHLQSALANQVQDRLSFIGASASTELLPALELASRFYIKGIF
                     SLFFLFAFLFIFFFYYSVRQNKQELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVL
                     LWIVSFQSVYEALLQRINFLVLKTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDL
                     FFTDILKGFAQTLIGLSGCCLILSLFPFTFFIHHLQSRRPGRNET"
     gene            608..1117
                     /locus_tag="ACHBKA_001719"
     CDS             608..1117
                     /locus_tag="ACHBKA_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445407.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1187..2635)
                     /locus_tag="ACHBKA_001720"
     CDS             complement(1187..2635)
                     /locus_tag="ACHBKA_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9445408.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2635..3351)
                     /locus_tag="ACHBKA_001721"
     CDS             complement(2635..3351)
                     /locus_tag="ACHBKA_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM9445409.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3332..3682)
                     /locus_tag="ACHBKA_001722"
     CDS             complement(3332..3682)
                     /locus_tag="ACHBKA_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM9445410.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3826..4599
                     /locus_tag="ACHBKA_001723"
     CDS             3826..4599
                     /locus_tag="ACHBKA_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9445411.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            4770..5136
                     /gene="ssrA"
                     /locus_tag="ACHBKA_001724"
     tmRNA           4770..5136
                     /gene="ssrA"
                     /locus_tag="ACHBKA_001724"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5570..5884)
                     /locus_tag="ACHBKA_001725"
     CDS             complement(5570..5884)
                     /locus_tag="ACHBKA_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445412.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            6170..6409
                     /locus_tag="ACHBKA_001726"
     CDS             6170..6409
                     /locus_tag="ACHBKA_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445413.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(6771..7031)
                     /locus_tag="ACHBKA_001727"
     CDS             complement(6771..7031)
                     /locus_tag="ACHBKA_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445414.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(7216..7713)
                     /locus_tag="ACHBKA_001728"
     CDS             complement(7216..7713)
                     /locus_tag="ACHBKA_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM9445415.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(7843..8553)
                     /gene="budA"
                     /locus_tag="ACHBKA_001729"
     CDS             complement(7843..8553)
                     /gene="budA"
                     /locus_tag="ACHBKA_001729"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM9445416.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(8566..10230)
                     /gene="alsS"
                     /locus_tag="ACHBKA_001730"
     CDS             complement(8566..10230)
                     /gene="alsS"
                     /locus_tag="ACHBKA_001730"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM9445417.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(10438..11100)
                     /locus_tag="ACHBKA_001731"
     CDS             complement(10438..11100)
                     /locus_tag="ACHBKA_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9445418.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(11110..11925)
                     /locus_tag="ACHBKA_001732"
     CDS             complement(11110..11925)
                     /locus_tag="ACHBKA_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445419.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(12186..12629)
                     /locus_tag="ACHBKA_001733"
     CDS             complement(12186..12629)
                     /locus_tag="ACHBKA_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM9445420.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(12763..13101)
                     /locus_tag="ACHBKA_001734"
                     /pseudo
     CDS             complement(12763..13101)
                     /locus_tag="ACHBKA_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(13089..13466)
                     /locus_tag="ACHBKA_001735"
     CDS             complement(13089..13466)
                     /locus_tag="ACHBKA_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9445421.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            13690..14883
                     /locus_tag="ACHBKA_001736"
     CDS             13690..14883
                     /locus_tag="ACHBKA_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9445422.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(15049..15471)
                     /locus_tag="ACHBKA_001737"
     CDS             complement(15049..15471)
                     /locus_tag="ACHBKA_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445423.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         4667 a         3073 c         2608 g         5272 t
ORIGIN      
        1 actctttacc atttgcaatc ggctttagcc aatcaagtcc aagatcggct ttcatttatt
       61 ggcgcttctg catctacaga gcttcttccg gctttagaat tagctagccg attttatata
      121 aaaggcatat tcagtttatt ttttctattt gccttccttt ttatcttttt cttttactat
      181 tcagtcaggc aaaacaagca ggaattgatc aattggcgcc tgactggtct ttctaaaacc
      241 aaattattcc tttttatttt ctggcagctg ttctttcctc tctttctttg ttgtatcttg
      301 gttttattgt ggatcgtaag cttccagtca gtttatgaag cgcttcttca aaggatcaat
      361 tttcttgtct taaagactac tgaactgcca gatatccgtg atatcttgac ttcggtaagc
      421 ggacttacaa ttccgatgga tcaacagact ttttttacca tcgatttcca aaatgatctt
      481 tttttcacag atatacttaa gggatttgcc caaactctta ttgggctcag tggatgctgt
      541 ctcattctta gtctattccc tttcactttt tttatccatc atttgcaaag taggaggcct
      601 ggacgtaatg aaacctgaat atttgattca tgctgaacac ttgaccgtta ttttgtgtcc
      661 tctcgaaaaa cagcataaaa tattggagca cttgaaaaaa gaggttcttg ttgcaaaact
      721 ggataatttt gcgatcatcc aaaaaaattg gccgatgttt ccttatctga atcttaaaga
      781 ccatgtcctg ttggatgtac cggaaaaaga aatcaaacaa gatcgtttgg cctatcaaga
      841 aaaacttgat atctctcctt ccttgcttaa ttgtgcggca gctgagttga cgtcttttga
      901 aaaagtaaag ctgcaattac ttcatgcctt gctttccaag cgaaacaata ttgttattga
      961 agatacattt gatgaactta gtgtattgga gatccaagag ctactccact tgctttctta
     1021 tttagctcat gaagaaaatc aaggtatctt actctttact catgatgcta ctattgctca
     1081 aagtccttat attgatcgtt tagagcccgc gggctaaatc aatcatttta tagtaaaaac
     1141 aagcggtata gaagatcgat tcatgatctt ttataccgct tgttttttac tcttttttga
     1201 tttctacgtc aggcaaacga acagcgaatt ttgttccttt tcctagttcg ctttctactt
     1261 ctaattttcc tttatgcaag cgtacaagtt gctgcacgat tggtaaacct aatcctgatt
     1321 caccatattt tgtatttttt cttgaaggat ctactttata ataacgatcc cagatacttt
     1381 tcatttgttg ttcagacatt ccaattcccg tatcttcaat cgtgatgatc gtttcaagat
     1441 agcctttttc taaagtaatc gtgatttggc cgttttcagt aaactgtatc gcgttttgaa
     1501 taatattgac catgatttgg acaaatcggt cataatcagc gtaaacatca atatcatcaa
     1561 cggctttcaa tgtcaatgta tctcctgctg cttctgcttt agcttctaac tgtgcaacaa
     1621 tatttttcaa tgtttctgtt ccattgaatt ttttaatgac catactgatt tgattcgtac
     1681 ggattttttc ataatccaag ttttcattca ccaaacggat cagacgctct gtttcatttt
     1741 tcatcaattt aatcgcgttt tctcgctgat tttcagggat tgcattgtat tctaaccctt
     1801 ccagaagtcc gttgattgtc gtaagaggtg ttctcatctc gtgagaagca tctgccataa
     1861 attgtcttcg acgattttct tgttcttcaa tttctgcttg agattctttc aatgaattcg
     1921 tcattttatt gaaatcatct gctaactcat caaattcatc tttgtcatgt acggggagct
     1981 gtacatcaaa attaccattc gtcacttctt tcgtcgcatt ccttaaccga ttgatacgct
     2041 taacttggaa tgctgcaaaa gcatagctgg caataactgc aacgatactg gaaaaaataa
     2101 atcctttgaa caggttcagt gtaattggac gaacgctgtt atcgatattt cgcgcaggtt
     2161 gactaactac aagtgctcca tagaattcat gattcaaatt aaatggcaca agtgcgtaag
     2221 aggtcgcctg tgcttgtcct aaaatatttt tattcgatgt gaatttttct tgacgtccat
     2281 tcctcagatt tttccattga tcagctgtta aggtaaaatt cagtaaagct gtattagtcg
     2341 gataaatgac acgttcgttt ttatcgataa atataaagtt cacattttgt tctgtcagta
     2401 cttgctctgt cagcagcaat gcattttgaa acgcctgatc ttgactaaac tgcgggagcg
     2461 tatcggcata ggtttgcgtt gtcttttcaa ctgattcagc atatccaaac agttgctggt
     2521 aattgttttc ttcaatcgtt tgttttgtca gttgtgtaaa agaaactcca acgatcaata
     2581 agatcaacgc gaccattccg caaaaagcta agagttgctg atacaaataa cgcattaggc
     2641 aacaccagaa tcatcgaatt tgtagccgac tccccaaaca gtttggatga cttgtggtcc
     2701 gaccttttcg attttttgtc gtaatttctt gatatgggcg tccactgtcc gttcatcacc
     2761 aaagtactga taatcccaga ctaattcaag caactgctcc cgagagaata cttgacgtgg
     2821 tttttttgct aaagtaaaca atagatcgaa ctctttaggc gtcagtcctt caattggccg
     2881 gttgtccaga tacgcttctc gtgttttcgt attcatcttg aaatgactag tctcgatatc
     2941 aaaatgatca tctgaaactg ctgcttcttc tcttgcttct gccaactcac tacggcgatg
     3001 caaagctttc attcgagcaa taagcgtcag aggactgaaa ggttttgtga cataatcatc
     3061 tgcccccatc tctaagccga tcacctgatc gctttcagaa tctctagctg tcagcatgat
     3121 aatcggcaca gtacttgaaa ctttgcggat ctctcttcct actagcatac cgtccagtgt
     3181 gggcaaattc aggtctagtg taatcatatc ccaatgttgt gggttttcta aaaaggcatc
     3241 gagcccttct tttccatcat acttgaaagt tgcttcccaa ccttcattta agaaaaacat
     3301 ctgcatcatt tcagaaacag attgattatc ttcaatcatt aaaatattca ttatcgttcc
     3361 ctccagtttc caggtcatat ttctgtaatt tcttttctgt cataataaaa ataggcataa
     3421 ttggaaatac tagcagaagc atttcaatca gaaaataatt cgtatgccca gatgtcttta
     3481 aataatagcc gatcaatgcc atcagtaagc ccataagaaa aaaccagttt ccgcttgtct
     3541 tttgagccag ccgccaatga gcgtctgttt ttgcggcaag aggcgaatga tacccataaa
     3601 aaggcagatt tccttttgag ggcagaatcc gaaaaagact gccaattatt aagaataaga
     3661 taccaatact gaaaaaaatc attctttccc tcctaaattc tcttcttata gtatactcta
     3721 aattgttgac tgaaccaata tgttcaggaa ttcttcacga aaggtttgtc ttttgttcat
     3781 tctttgttaa gataaactta ttccaataaa aaaggagttt tatttgtgtc aaattatcgt
     3841 gcaatcactt tctttgatct tgatggaaca ttactagatg cgaaatcaaa aatcacaccc
     3901 gaagtggcaa aagctatgaa cgcattaaaa gaaaacaatg ttctccccgt catcgctaca
     3961 ggacgtacag aagcagaaat ccactctatc atggaagatg cgggcattac ttctgcggtc
     4021 accatgaatg gttctttcat tcgagtagac ggcgaagaaa tctattctca aacattttct
     4081 tcagaagaat gtcaaaaaat gtatgatcat gtacagttga acggtcatga actttcttac
     4141 tataacgatc agaaaatctg gtgtaccggt catagccaaa caatgatcaa tgcttacgac
     4201 ttcattcatt cagatgttcc ggaaattgat cctttaggct ttaaagaaaa cacagtaaat
     4261 atgttgctga tcttatctca atcaggtgac gaatactatc acgaacattt tcctgagcta
     4321 actttttatc ggaatggtcc ttactcgatc gatacagtga agaaaggaat ctcaaaaggg
     4381 gcaggtgtca aacgtctgca aaaagaactt cagctggaaa aagttcctac ttttggattt
     4441 ggcgatggtc cgaatgattt tgccttgttg gaagcttgcg acaacaaaat cgctatgggc
     4501 aacgcctatg acggattaaa agaactttct acctttatca caaagaaaaa tacagaaggc
     4561 ggaattgtcc acgcattgaa gtattttgat ctgatttaag atcttttttt gaagctacct
     4621 cttttcttaa gaaaatcgct tattttttct taagaaaggg gcagttatta tataaataat
     4681 gggatatgat tcgctttttt attgaattta tcccaatata tgctatacta attttgtatg
     4741 aaaaatacac gtaacccttg ttagctaacg gggacgttac ggattcgaca ggcacagtcg
     4801 agcttgaatt gcgtttcgta ggttacgtct acgtaaaaac gttacagtta aatataactg
     4861 ctaaaaacga aaacaactct tacgctttag ctgcctaaaa acagttagcg tagatcctct
     4921 cggcatcgcc catgtgctcg agtaagggtc ctaactttag tgggatacgt ttcaactttc
     4981 cgtctgtaag ttgaaaaaga gaacatcaga ctagcgatac agaatgcctg tcactcggca
     5041 agctgtaaag tgaatcctta aatgagttga ctatgaacgt agatttttaa gtggcgatgt
     5101 gtttggacgc gggttcgact cccgccgtct ccatatatgt atggttgaga cagataaaca
     5161 tgtatgaaaa acgttgttaa atcaacgttt ttttattttg gtcaaggaga gaaaagtaga
     5221 gaaatacaaa attttttgca ttgattttgc attcaaaaaa acattcaaac atttagaaat
     5281 ccccaatacg tattcaggaa aatctatttt aaggtttaca aaaagaactt ttgttcgtac
     5341 actctttttg aggtgaactt ttatgctgat ggaaggaaaa acacaattat ggtttaagca
     5401 tgttccttca aataagtttt ttaattataa actctagaga aattaattca tcctaacata
     5461 aaataattgt tcactcaaaa gtttttctag aacaattaaa acagcaggct tcacattcgc
     5521 tatacatttg taagaagtaa taattcctgt actaaaaggc ataattgctt taattatttt
     5581 ttgacttctc ttcaaacaca agaagtagtt gtgtaataat tgcagaaata aggattccaa
     5641 taatagatat aaaatttgac atggaaacgt ctataacatt aaaagaataa agtgttgaag
     5701 aaagtaaact gattgaaata acccctccgt aaaaaatagt ctgtgaatat acccactttt
     5761 tttcagaaga cgtacttcgt ttggttctaa aacctatccc tgcatttcta ctcttatcaa
     5821 aaaaccaaat atttagaagt aataatattg ctattattaa gataaaatta ctgtaaaaat
     5881 ccaaaagcta tcctcctagc aactatttaa atacccaaaa aaattacctc tagataatat
     5941 catacctacg ttttcactta taaatttttt cataaaaaaa gcgtttagag gcataatcgt
     6001 taatacagat ggtaaccaat gtatctggtt tgaactggga tatcaccctc tccatatatg
     6061 tattactaag actaatagat actataaaag acttgatagt tttgcattga ttttgcctaa
     6121 tactgagcat aggtgtatgc taaacgatac tataaggagt gtgtacataa tgaaaagaat
     6181 tctgtttatc agtttatcaa atttattgtt acttactgct tgtgggactt ctgcatcaaa
     6241 gaacatacct tcagatacgc aaatttcgaa tagtatcgat tcaacaattt ctacaaatga
     6301 gaaaaattct ccggtagaaa cttccgatat aaaagtttct acctctgcaa aaaacagggt
     6361 aaagaaactg tcagcacgtc tcctttattt caaacaaact ctgaattagc ttcttcatct
     6421 tcttcagcaa ataatgatga tagtttcgcg tttactccta actaaatgca acctttacaa
     6481 atgctttacg ataacaagcc tgagctaaat gatcctgata tcagttttgc attttttgaa
     6541 atgattggat cggatgatct ttttaaaact actagtttgt ctatccgaaa ataggacagt
     6601 tctggcagta taggttttta tcgagtttct ccaccaggcc tcgttacaga gacagattct
     6661 ctaggcgaac cttttgaata aatcaaaaat catgaacaat cactgccaac taaataaagt
     6721 gccttcaaaa gcttggtctt ctcgcttttg aaggcacttt atttaaatct ttatgaaact
     6781 aaagtatgtt catatttgtt gctatagtta tatagatatt ctgttgtttc caactcataa
     6841 acgtgagtat attttgccac tacttttccg catttttcta atatttcatt gcgatcgacg
     6901 tcatgatatt cgtctacgca gatcacaaca caattttcta atttactgat tacttctcca
     6961 accacttttt gtttcaagcc aataggttgg caaatatatt tttttcctaa aatgatagtt
     7021 tcgttattca tatttcatcc atccttaatg ttttttcatt attcaaaatg ataataccat
     7081 taaaatatga ttgtgaaaat caataacact tgtgatttta taaaaaaata aaaaaaccga
     7141 aaaacagaaa tgaccactgt ctttccggct ctttttattc ttgatttctc ttcttttttg
     7201 tttcaataga cgattttagt tattaataaa tgaaaaagca tttatattaa aatttgctaa
     7261 taaaagatct acatattttt ctagaaatgc ttgatcgatt tcatcatacc ctttcgttac
     7321 tccgcaatcg atatctaaaa caccgattaa attaccttct tttaccattg gcactacgat
     7381 ttctgattgt gcggctgaat cgcaggaaat gtaatttgca tgggttttga catcatcaac
     7441 gatcaatgtt tcttgttttt cagctgattc tccacaaact ccttttccca tcttgatacg
     7501 tgtgcaagaa actcgccctt ggaacggtcc taaaatcaat tcattaccat cgaataaata
     7561 atatcctgca aagacagtct caggcaaagt ctcattcaac aatgcagatg aatttgctaa
     7621 atttgctaac cagttatctt ctgcttctaa taatccttgt tgctgtaata ataaaatttc
     7681 gtatgcatct tttttctgtt tttctgtcca catgataaaa accgctcctt tttgatctat
     7741 ggagaagatt atagctcagt ttttttgtaa acgctaggtt tcctcctcaa aaaaacgaga
     7801 tacgtcaggt taatcacctt gaacgcatct cgctgtcttt ttctattctg cttgttggat
     7861 ttctgctgct aaatcagcat aatcaatcgt ttcgtgaaca aaagtcggat catcaactgg
     7921 aaaatgctgt acaagggacg agatgcggct aatctcaact tttccttcag ccacttcaaa
     7981 atccattaca tgtccgccaa atgtatgtgt atcatcgata aaatgcaggt gaaatcccgc
     8041 tgcagcaact ccttggaata attcaggcgt aaagaacccg actaatgttc cttggatatt
     8101 gctttgttgg aattctggtt gagactctga aatttttgct agtcttctgt aaggtttttc
     8161 ttgcttcggc atgatacgga tgtgcatatt tttgaataat ccagttatct tcactgccaa
     8221 aaataggttt tccccggctt ctcttactaa tatgtctttt ttcaattgtt ctccatctgc
     8281 agtttttgag acagaaaaac tagtatctgg cgtaaaatct gtaattgctg catagggggt
     8341 aagttcactt ccatctaatt gggcaactga gccatctgat cttccttgat aagctacgcc
     8401 atctaaaaag atgacttcac catcaagccc atgcaatgtt cccaatccca gagttccttt
     8461 ttctaatata tgagcaatcg tttctgttcc atccatcaag ccggccatca aagctcctaa
     8521 tgtcccgtgc tggtataaga ttttttcagt catgtcgaca cttccttaat agaattgatc
     8581 tggtaagatt gtttctccca atttctcatt atcacggtaa tcaatcggaa tatcaataat
     8641 tacgggtcct tctgtcgcaa atccttcttc taaagctttt tctaattcct ctacacttgt
     8701 agctcggatt cctttagcgc caaaagcttc tgcatatttc acaaaatcaa ctggaccaaa
     8761 gtctacacct gacgcccgct gatatttcat tttttcttgg aactctacca tattgtagtg
     8821 tccatcattc caaatcaaat gaacgatatt caatttttta cgtaccgccg tttctaaatc
     8881 ctgggcagag aataaaaatc ccccatcgcc ggaaactgat acgatttgtg tatttggacg
     8941 caccaatgca gcagaaatcg cccatggcaa tgcaacacca agcgtttgca taccattact
     9001 gaataacaaa tgacgaggtt cataacttct aaagtggcgt gccatccaaa tataatgact
     9061 acctacatct acggtaacag tcatatcatc tgttactttt gactgaagcg tgttaataac
     9121 ttctaaagga tgcaagatac ctgcttctcc ttttgattga acaatgtccc gctccgttaa
     9181 ttttgcttga agagatgcta aatattcttt tgcatcttct gacacttgtt gaggctctaa
     9241 tgaacctgtc aataaatcaa gagtggctga gatatcaccg atcaattctc tttcaggctg
     9301 cataaaagga tcgatttcag ctggcgcttc atcgatcaca atgatccgcg catctttttc
     9361 cgcattccag tttcttgctt catattcgat tggatcatag ccaattgcaa taactagatc
     9421 acttcgtttc aaaagcatat ctcctggttg gtttctaaat aaacccacac gaccaaagaa
     9481 atgcgcttct aattcacgtg agatcactcc agctgcttgg aatgtctcta caacaggcaa
     9541 tgctgtcttt tcaactaatt gcctgatggc taacgtttct ttttcacttg atcctcgcat
     9601 acctactaat agtaccggta gttttgcttc tctaatgcgt tcagctaggt aacggatatc
     9661 atctgcagaa gcagaaccta atttaggatc gctcaatggc ttgatgacat taccttggac
     9721 aggtgcatcc actacatctt gcgggatact gataaagctc gcgccttttt ttgaactctt
     9781 agccatacga taagcattgg caatcacttc tgaaattgtt tctggatctt ggatttctgc
     9841 actgtatttt gtgattggct ggaacagtgc tgcattatcc atgctttgat gggtcaactt
     9901 caatagatcg cttcgtttta cttgtccggc aattgctaaa actgggtcac cttctgctgt
     9961 cgctgttacc aaacctgtag ctaagttact agcaccagga ccacttgttg cgataactac
    10021 tcctggctct cctgtaatac gtccgattgc ttgtgccata aatgcagcat tttgttcgtg
    10081 acgagtcacg attaattcag gaccttgatc ctctaattca ttaaatacac catcgatttt
    10141 tgcacctggt ataccgaata cataatcaac atgatgatta atcaaacttt ctactacaga
    10201 tttcgaacct tgtttcattt tttcagccat tgaacatcgt cctttttatg ctttattttt
    10261 aattattagt tagaaataat cacaaataag tgatacagtt tctaaacctg tttcatacaa
    10321 aagtatagca cgttttattt catttgttta gccatatgtt tcaaaaaatg aaaaagaatg
    10381 acagttttta cataaaaaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta
    10441 cagatccata gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga
    10501 aggacctagt agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa
    10561 tagtaaaaaa tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg
    10621 tagaacttga tttggtttcg acgctggatt tctcacctga aaataccagc agttggtgct
    10681 aaaggaaact gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga
    10741 aaaacgtata aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag
    10801 ccaataaaaa aatcccttta gccaaaattg gcgaaacatc tttatgaaca aacgtactgg
    10861 tgaaaaacta ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg
    10921 accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag
    10981 gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga
    11041 ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat
    11101 aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat
    11161 cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc
    11221 gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc
    11281 cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat
    11341 gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc
    11401 ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta
    11461 atattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca
    11521 caacgtatct gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg
    11581 ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt
    11641 tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac
    11701 aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac
    11761 cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc
    11821 attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc
    11881 taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat
    11941 ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa
    12001 tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt cttatttttg
    12061 taagcgctta tttaacaagt tatgtaaatc tctttatttt tttgatttaa aaatgaaaaa
    12121 atacaaaagc aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat
    12181 acttattact gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat
    12241 aaaatccgat tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga
    12301 acgaatgtca ctcgctttga atgatgactt acagcacctt ctatgactaa agcagcacct
    12361 acctgattct caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc
    12421 aactgctcct tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt
    12481 gagatcaaaa gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa
    12541 acagaatttt ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg
    12601 tatttctctg tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt
    12661 cactccgtga tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca
    12721 cggatcaact tttctagtaa tagtgccaat gtattcgaac tatcagcttc cacacagccg
    12781 atcgtaaatc ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga
    12841 ttcgttaaat ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa
    12901 gatcattttg cggttttcgc gtacaaacag ttctgttcct agttactctt ctaactcctt
    12961 cagttgtcgg cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt
    13021 tccttcttct gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct
    13081 tgattttttt acttgataca ttccatttta tttctagata acacacgatc atatttcttg
    13141 atttttcctt gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt
    13201 tcactttctt caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat
    13261 aggcgaatat attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta
    13321 aagtcgaccc attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta
    13381 actggtggaa tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt
    13441 tcttcactga cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt
    13501 acttatgtat tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta
    13561 ttaaaactaa gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc
    13621 ttgttttttt attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg
    13681 ggtataaaaa tgacaacgta tcagcgaaag gtattagtat cgacatcggc gggaattgct
    13741 ttagagaata tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt
    13801 catattagtg gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt
    13861 ggaggtattt tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa
    13921 acggtccttc tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg
    13981 gtttatctct ttcgctttat cgcaggtgta ggagcgggag gagaatatgg ggcgtgtatg
    14041 tccttgattt ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt
    14101 attggtgcgc aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg
    14161 tttggttgga agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt
    14221 cgtcgcggct tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc
    14281 aaacttagca gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc
    14341 atggtaactg tccaaattgc cggctatttt gggttaatga attggctacc ttcaattatg
    14401 caaaatcgat tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta
    14461 ggcatgtctt tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta
    14521 gccttttctt tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat
    14581 agccagtgga gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat
    14641 ggtggatatg gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat
    14701 aatttcgtta tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc
    14761 ttgatggatc attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc
    14821 agtctgctgg tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat
    14881 tgatagttga gtctgtaaat gacaagaaag aggtgtggaa aaagcttgca tctgcggtaa
    14941 taagaccagc caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg
    15001 atgcagggca gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac
    15061 gtccgtcatt tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact
    15121 cgtatattga gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc
    15181 aatcaaatga taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa
    15241 gttctcctgc ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc
    15301 aggatattgt tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga
    15361 taccttcaat tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa
    15421 atgaatcatt ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc
    15481 tcctgtatct actttactat actacataca agaaaaaaac ggatacaaat atcatattgt
    15541 cacaccgtca atagtgcata aacctgttat tatttctctt tttttcctaa gtattctatt
    15601 gatatgaatc atttaatcgt
//
LOCUS       DAYYOA010000062        15084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000062 DAYYOA010000000
VERSION     DAYYOA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.062"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1246)
                     /locus_tag="ACHBKA_001738"
     CDS             complement(167..1246)
                     /locus_tag="ACHBKA_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9445424.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            1354..2805
                     /locus_tag="ACHBKA_001739"
     CDS             1354..2805
                     /locus_tag="ACHBKA_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9445425.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3154..3786
                     /locus_tag="ACHBKA_001740"
     CDS             3154..3786
                     /locus_tag="ACHBKA_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM9445426.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4374..5813
                     /locus_tag="ACHBKA_001741"
     CDS             4374..5813
                     /locus_tag="ACHBKA_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9445427.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            6108..6326
                     /locus_tag="ACHBKA_001742"
                     /pseudo
     CDS             6108..6326
                     /locus_tag="ACHBKA_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            6575..6790
                     /locus_tag="ACHBKA_001743"
     CDS             6575..6790
                     /locus_tag="ACHBKA_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445428.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(7007..7263)
                     /locus_tag="ACHBKA_001744"
                     /pseudo
     CDS             complement(7007..7263)
                     /locus_tag="ACHBKA_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7450..8361)
                     /locus_tag="ACHBKA_001745"
     CDS             complement(7450..8361)
                     /locus_tag="ACHBKA_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9445429.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8380..9384)
                     /locus_tag="ACHBKA_001746"
     CDS             complement(8380..9384)
                     /locus_tag="ACHBKA_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9445430.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9381..11504)
                     /locus_tag="ACHBKA_001747"
     CDS             complement(9381..11504)
                     /locus_tag="ACHBKA_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9445431.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11509..13956)
                     /locus_tag="ACHBKA_001748"
     CDS             complement(11509..13956)
                     /locus_tag="ACHBKA_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9445432.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13943..14332)
                     /locus_tag="ACHBKA_001749"
     CDS             complement(13943..14332)
                     /locus_tag="ACHBKA_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9445433.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14392..14895)
                     /locus_tag="ACHBKA_001750"
     CDS             complement(14392..14895)
                     /locus_tag="ACHBKA_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9445434.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
BASE COUNT         4525 a         3032 c         2826 g         4701 t
ORIGIN      
        1 acaggtaact catttgtttg ataccgtaac tgcctcgtta ttctaaatca gtgaggcctt
       61 gataatgaac tgaggtatga tagtcttcag acgattctgg ccccaggcca gctttccaga
      121 catgaagcca tgtcccgagt gaggatcgtt atcaaggaat aatgggtcaa gccaccaacc
      181 gttcttgctt gaatgcctgg acgaatgtat catgtggtgt cgcataaccc aaaattttcc
      241 gcgggtaatc gttcatccaa cgctggatcc gcaaaatctg gttctcgctc acctcactaa
      301 tcgggcttcc cttggggacg aaacggcgaa ttaatttgtg ctggttttcg ctcgtcccac
      361 gctcccaaga agcatagggg tggctgaaat agacgtcagc tacatcttgc agagcatcgt
      421 gtagcccaga aaactccgag ccattgtcgg atgtaacggt cttaaataat tgatcgaagt
      481 tagcgccagc ccgttctctt agctgttgaa ccgcttggtc caccgattcg gtgtccttcc
      541 cgttcaactt taggatgact tcaaagcgtg tctgccgttc agccagcgtc agcagcacgg
      601 catccgactt ggtcttgttt cccactaccg tgtcgatttc ccaatgaccg aacgtttccc
      661 gtgtttcaac ttctttaggg cgttcttcaa ttgacgtccc gagaacacgc ttgttgggac
      721 ggtgtcctgg gcggaccgct ttgattttac gggacagttt ttcaagcaag tccatgttct
      781 ttgttcgcat gattccgcgg tcaatccatt gataaagagc ggttgtgcca ggaatgatgg
      841 cacaatcgaa cagctcgtgt tttctggcaa atccgaccac gacgtccggc gaccattttt
      901 catttaacat tttgtcatct gcccaatcaa tgaaggcatt tgtgttcgcc catttggggc
      961 gtctgccaca gttcagtcgt tgcttttcat agaaggcctg tcctgcatca gcgtcataaa
     1021 cagaatagta ggtgtcatac acttttccat tttgcgtttg gcgttttaac tgggtgatgg
     1081 ttccgcgatc aatctcattg tttatggttt gcggaacacg tcccagcact ttggcaacct
     1141 gacggttgga atagccttcc tttttcagga tagcgatttg gcaacgttct gagtaggaaa
     1201 gatgttttcc cttgcgtgaa actgtgttat catgggtttg cgtcatgtga attcatcctt
     1261 gtctattgag agttgtggta acttcaatat aacatggaat tcacgtggcg ttttttttat
     1321 tttaaatggc taagttcatt ataaaatccg ccaatgaaaa aatctttaaa acttgcatca
     1381 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     1441 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     1501 gtaacagcag agagtagtga acttttgaca acttcatcaa gtgaaagtag tacgacttca
     1561 aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
     1621 tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttcg
     1681 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
     1741 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
     1801 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
     1861 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
     1921 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
     1981 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
     2041 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
     2101 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
     2161 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
     2221 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
     2281 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
     2341 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
     2401 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
     2461 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
     2521 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
     2581 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
     2641 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     2701 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     2761 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     2821 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     2881 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     2941 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     3001 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     3061 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     3121 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     3181 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     3241 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     3301 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     3361 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     3421 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     3481 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     3541 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     3601 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     3661 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     3721 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     3781 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     3841 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     3901 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     3961 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     4021 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     4081 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     4141 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     4201 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     4261 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     4321 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     4381 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     4441 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     4501 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     4561 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     4621 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     4681 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     4741 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     4801 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     4861 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     4921 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     4981 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     5041 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     5101 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     5161 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     5221 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     5281 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     5341 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     5401 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     5461 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     5521 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     5581 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     5641 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     5701 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     5761 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     5821 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     5881 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     5941 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     6001 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     6061 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     6121 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     6181 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     6241 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     6301 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     6361 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     6421 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     6481 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     6541 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     6601 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     6661 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     6721 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     6781 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     6841 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     6901 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     6961 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     7021 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     7081 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     7141 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     7201 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     7261 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     7321 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     7381 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     7441 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     7501 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     7561 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     7621 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     7681 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     7741 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     7801 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     7861 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     7921 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     7981 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     8041 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     8101 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     8161 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     8221 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     8281 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     8341 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     8401 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     8461 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     8521 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     8581 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     8641 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     8701 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     8761 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     8821 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     8881 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     8941 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     9001 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     9061 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     9121 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     9181 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     9241 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     9301 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     9361 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     9421 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     9481 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     9541 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     9601 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     9661 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     9721 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     9781 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     9841 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     9901 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     9961 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
    10021 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
    10081 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
    10141 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
    10201 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
    10261 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
    10321 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
    10381 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
    10441 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
    10501 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
    10561 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
    10621 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
    10681 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
    10741 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
    10801 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
    10861 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
    10921 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
    10981 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
    11041 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
    11101 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
    11161 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
    11221 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
    11281 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
    11341 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
    11401 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
    11461 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
    11521 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
    11581 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
    11641 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    11701 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    11761 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    11821 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    11881 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    11941 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    12001 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    12061 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    12121 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    12181 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    12241 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    12301 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    12361 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    12421 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    12481 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    12541 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    12601 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    12661 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    12721 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    12781 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    12841 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    12901 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    12961 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    13021 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    13081 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    13141 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    13201 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    13261 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    13321 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    13381 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    13441 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    13501 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    13561 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    13621 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    13681 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    13741 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    13801 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    13861 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    13921 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    13981 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    14041 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    14101 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    14161 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    14221 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    14281 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    14341 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    14401 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    14461 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    14521 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    14581 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    14641 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    14701 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    14761 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    14821 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    14881 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    14941 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    15001 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    15061 aattgccagt aagactaagg attg
//
LOCUS       DAYYOA010000063        14815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000063 DAYYOA010000000
VERSION     DAYYOA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.063"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHBKA_001751"
     CDS             complement(296..1024)
                     /locus_tag="ACHBKA_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445435.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHBKA_001752"
     CDS             complement(1002..2576)
                     /locus_tag="ACHBKA_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9445436.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHBKA_001753"
     CDS             2805..3743
                     /locus_tag="ACHBKA_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9445437.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHBKA_001754"
     CDS             3755..4666
                     /locus_tag="ACHBKA_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445438.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHBKA_001755"
     CDS             4690..6162
                     /locus_tag="ACHBKA_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9445439.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHBKA_001756"
     CDS             6213..6824
                     /locus_tag="ACHBKA_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9445440.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHBKA_001757"
     CDS             6854..8767
                     /locus_tag="ACHBKA_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM9445441.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHBKA_001758"
     CDS             8895..11876
                     /locus_tag="ACHBKA_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM9445442.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHBKA_001759"
     CDS             11873..13534
                     /locus_tag="ACHBKA_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM9445443.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHBKA_001760"
     CDS             13574..14506
                     /locus_tag="ACHBKA_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM9445444.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAYYOA010000064        14514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000064 DAYYOA010000000
VERSION     DAYYOA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.064"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(426..4079)
                     /gene="rpoC"
                     /locus_tag="ACHBKA_001761"
     CDS             complement(426..4079)
                     /gene="rpoC"
                     /locus_tag="ACHBKA_001761"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM9445445.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(4158..7775)
                     /gene="rpoB"
                     /locus_tag="ACHBKA_001762"
     CDS             complement(4158..7775)
                     /gene="rpoB"
                     /locus_tag="ACHBKA_001762"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9445446.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8503..9471
                     /locus_tag="ACHBKA_001763"
     CDS             8503..9471
                     /locus_tag="ACHBKA_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9445447.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9489..10394
                     /locus_tag="ACHBKA_001764"
     CDS             9489..10394
                     /locus_tag="ACHBKA_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9445448.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10391..11197
                     /locus_tag="ACHBKA_001765"
     CDS             10391..11197
                     /locus_tag="ACHBKA_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9445449.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11354..12322
                     /locus_tag="ACHBKA_001766"
     CDS             11354..12322
                     /locus_tag="ACHBKA_001766"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM9445450.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12639..13163)
                     /locus_tag="ACHBKA_001767"
     CDS             complement(12639..13163)
                     /locus_tag="ACHBKA_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM9445451.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13450..>14514
                     /gene="scm"
                     /locus_tag="ACHBKA_001768"
     CDS             13450..>14514
                     /gene="scm"
                     /locus_tag="ACHBKA_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9445452.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4125 a         3113 c         2838 g         4438 t
ORIGIN      
        1 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
       61 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
      121 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
      181 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
      241 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
      301 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
      361 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
      421 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
      481 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
      541 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
      601 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
      661 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
      721 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
      781 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
      841 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
      901 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
      961 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
     1021 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
     1081 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
     1141 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
     1201 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
     1261 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
     1321 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
     1381 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
     1441 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
     1501 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
     1561 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
     1621 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
     1681 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
     1741 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
     1801 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
     1861 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
     1921 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
     1981 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
     2041 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
     2101 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
     2161 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
     2221 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
     2281 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
     2341 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
     2401 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
     2461 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
     2521 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
     2581 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
     2641 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
     2701 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
     2761 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
     2821 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
     2881 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
     2941 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
     3001 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
     3061 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
     3121 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
     3181 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
     3241 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
     3301 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
     3361 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
     3421 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
     3481 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
     3541 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
     3601 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
     3661 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
     3721 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
     3781 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
     3841 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
     3901 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc acgttcaggt
     3961 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
     4021 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
     4081 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
     4141 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
     4201 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
     4261 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
     4321 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
     4381 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
     4441 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
     4501 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
     4561 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
     4621 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
     4681 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
     4741 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
     4801 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
     4861 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
     4921 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
     4981 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
     5041 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
     5101 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
     5161 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
     5221 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
     5281 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
     5341 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
     5401 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
     5461 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
     5521 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
     5581 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
     5641 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
     5701 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
     5761 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
     5821 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
     5881 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
     5941 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
     6001 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
     6061 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
     6121 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
     6181 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
     6241 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
     6301 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
     6361 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
     6421 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
     6481 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
     6541 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
     6601 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
     6661 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
     6721 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
     6781 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
     6841 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
     6901 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
     6961 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
     7021 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
     7081 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
     7141 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
     7201 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
     7261 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
     7321 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
     7381 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
     7441 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
     7501 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
     7561 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
     7621 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
     7681 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
     7741 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
     7801 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
     7861 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
     7921 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
     7981 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
     8041 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
     8101 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
     8161 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
     8221 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
     8281 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
     8341 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
     8401 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
     8461 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
     8521 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
     8581 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
     8641 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
     8701 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
     8761 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
     8821 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
     8881 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
     8941 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
     9001 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
     9061 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
     9121 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
     9181 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
     9241 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
     9301 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
     9361 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
     9421 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
     9481 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
     9541 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
     9601 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
     9661 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
     9721 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
     9781 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
     9841 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
     9901 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
     9961 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    10021 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    10081 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    10141 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    10201 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    10261 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    10321 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    10381 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    10441 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    10501 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    10561 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    10621 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    10681 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    10741 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    10801 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    10861 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    10921 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    10981 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    11041 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    11101 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    11161 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    11221 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    11281 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    11341 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    11401 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    11461 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    11521 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    11581 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    11641 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    11701 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    11761 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    11821 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    11881 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    11941 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    12001 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    12061 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    12121 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    12181 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    12241 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    12301 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    12361 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    12421 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    12481 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    12541 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    12601 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    12661 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    12721 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    12781 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    12841 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    12901 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    12961 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    13021 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    13081 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    13141 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    13201 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    13261 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    13321 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    13381 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    13441 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    13501 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    13561 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    13621 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    13681 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    13741 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    13801 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    13861 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    13921 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    13981 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    14041 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    14101 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    14161 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    14221 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    14281 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    14341 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    14401 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    14461 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DAYYOA010000065        14157 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000065 DAYYOA010000000
VERSION     DAYYOA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.065"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..258
                     /locus_tag="ACHBKA_001769"
     CDS             <1..258
                     /locus_tag="ACHBKA_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445453.1"
                     /translation="YFFTDDDIFLILAILFLVSSNFSDNLIEVLTEPEIRSMTDEEQQ
                     IIKAMISENKKDVVVIKKIREYTNLGVLQAKKVYEKKKKGL"
     gene            435..1385
                     /locus_tag="ACHBKA_001770"
     CDS             435..1385
                     /locus_tag="ACHBKA_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9445454.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1903..2313
                     /locus_tag="ACHBKA_001771"
                     /pseudo
     CDS             1903..2313
                     /locus_tag="ACHBKA_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2756..3568
                     /locus_tag="ACHBKA_001772"
                     /pseudo
     CDS             2756..3568
                     /locus_tag="ACHBKA_001772"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3563..3727
                     /locus_tag="ACHBKA_001773"
     CDS             3563..3727
                     /locus_tag="ACHBKA_001773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445455.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            3667..3789
                     /locus_tag="ACHBKA_001774"
                     /pseudo
     CDS             3667..3789
                     /locus_tag="ACHBKA_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(4351..4857)
                     /locus_tag="ACHBKA_001775"
     CDS             complement(4351..4857)
                     /locus_tag="ACHBKA_001775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9445456.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RYWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRNSKILVPINILKSKISSLVSPLTEEL
                     QKIAMKVM"
     gene            complement(4862..5629)
                     /locus_tag="ACHBKA_001776"
     CDS             complement(4862..5629)
                     /locus_tag="ACHBKA_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9445457.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6119..6544
                     /gene="lacA"
                     /locus_tag="ACHBKA_001777"
     CDS             6119..6544
                     /gene="lacA"
                     /locus_tag="ACHBKA_001777"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFM9445458.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6561..7076
                     /gene="lacB"
                     /locus_tag="ACHBKA_001778"
     CDS             6561..7076
                     /gene="lacB"
                     /locus_tag="ACHBKA_001778"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFM9445459.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7087..8019
                     /gene="lacC"
                     /locus_tag="ACHBKA_001779"
     CDS             7087..8019
                     /gene="lacC"
                     /locus_tag="ACHBKA_001779"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFM9445460.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8022..9002
                     /gene="lacD"
                     /locus_tag="ACHBKA_001780"
     CDS             8022..9002
                     /gene="lacD"
                     /locus_tag="ACHBKA_001780"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9445461.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9030..9347
                     /locus_tag="ACHBKA_001781"
     CDS             9030..9347
                     /locus_tag="ACHBKA_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9445462.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9347..11053
                     /locus_tag="ACHBKA_001782"
     CDS             9347..11053
                     /locus_tag="ACHBKA_001782"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9445463.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11195..12601
                     /gene="lacG"
                     /locus_tag="ACHBKA_001783"
     CDS             11195..12601
                     /gene="lacG"
                     /locus_tag="ACHBKA_001783"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9445464.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12624..12896
                     /locus_tag="ACHBKA_001784"
     CDS             12624..12896
                     /locus_tag="ACHBKA_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFM9445465.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12917..13816
                     /locus_tag="ACHBKA_001785"
     CDS             12917..13816
                     /locus_tag="ACHBKA_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM9445466.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4615 a         2350 c         2737 g         4455 t
ORIGIN      
        1 tactttttta ccgatgatga tattttttta attttagcaa tactttttct tgtttcatct
       61 aattttagtg ataacttaat tgaagttctt acagaaccag aaattcggtc tatgacggat
      121 gaagaacaac agattattaa agctatgatt tcggaaaata agaaagacgt agtagttatc
      181 aaaaagatta gagagtatac aaatttaggt gttttacaag ccaaaaaagt atatgaaaaa
      241 aaaaaaaagg gtctataatt acaaattaga taatacatta gaagctggag acaagattgt
      301 ctagataatg tgtgagataa aatttttggc aggttatcat acataagaca aaatgttttt
      361 aaaactgata acgtatttat tccacaacaa ttacgcattt atttctgaga aaaatataaa
      421 gcaggaggat tacaatgctg aaaacttatt ccttaacaaa agatacatta ttagtttcgt
      481 cagaatcaaa tatcaaaaat tcttctgttg tttatgttta tggtgacgaa cgagaatata
      541 tcgaatcttc tgaacataaa tatgacttta aaataggcaa tatactaacg tttgatgatc
      601 gagttgcata tgacacgatg atagataaga acgatgaaaa aagtttgctc gtaagttttt
      661 tgtttcccga ggtacatgaa ggtgggcatc tagataccct gacgacatct gttgttttct
      721 tggtatttaa aaataagttg ataattttta caaaacaaaa attaaaattc attcccgaac
      781 tcttatccaa tatggtttta cgtgatgtca atcatttcgt ggaggaagta cttttaaaga
      841 tcatgcaaaa agatttctat atgatgctta acgaccttcg tggagtcaag gaaaaaattg
      901 atgatttaga ctcagaaata tcaacgtccg gttcactcaa accaacgttt ggtgatttat
      961 tgactcttca aaaatacatg atagctttgt caacaactta taaagctaac cataaagcgc
     1021 ttgatttcat taaaaaaaat ttcacaacta ttgacgatat tgattttagt actaaaaaaa
     1081 taattgatga tttatacgat acaagtgata caatggatag aatcatttta agttacagcc
     1141 aatatttgga caaattggaa aatatgataa acaatatgat ttcgtatcag ctaaacatga
     1201 ttatgaaaac cctaaccgag atatcaattg ttttgactat tccagcaatt atttttggct
     1261 tttggggaat caatgtccat gtcccgtttg aaaagtcttc ttatggtgtt ttagccgtat
     1321 tgattatctc tgctatacta agtctaattt gctggctttt gatgcgacgc aaaacatatt
     1381 tgtaaagtat ttgtgatacc ctaatatgat ttaaagcaga atttctttgt tgacttttta
     1441 aaaggctgat tcctttcaat aatttaatag cttcagcggc tgctcagaag tcaataaatt
     1501 ggactatttt tattcagtta cttaggcaac atttgagata cgattgcgat tataaaaatt
     1561 gatataccaa ccaatggctg cttggaagac tgacatatta gcctcgatat catcgaataa
     1621 ctaaatacag aaaaatacaa taaatattat caaactaggg actatactag aaaacatcag
     1681 tacattcctt aactcagggt ctataattag aaaatcgccc ctttgaaaac tgattaaaac
     1741 agcccccatt atctagtgac tagataatgg gggctgtttt tttgttaagt gatataataa
     1801 accataaaag aagtttagaa atacggtcct ggtcatcaat aaataaacaa tttaattaat
     1861 tatttattta attatttaat ttcagaaagg tatacatgaa tcatggtgca acaaattgtc
     1921 ctacccatca aagactccag cgtcttaaag atggtacaag atacacttct cgatagtttc
     1981 cgtgctggtc gccgaaatta caccgttttt caagttggta aggccacgct actacgtgtg
     2041 agtgatgtca tgacattaaa aaaatcagat gtctataatc cagacggctc tgtcaaaaat
     2101 gcaaccttta ttcatgataa aaagaccggt aaagcaaaca cgttatattt aaagcctgtt
     2161 cagcaagact tgttgcaata tcatgattgg ctggcccaag aaaatatcaa ttctgagtgg
     2221 ttatttccct cgacagccca ttatgatctt catatcactg agaagcagtt tcataaggtg
     2281 atggcgcatg ttggtgatct cttaggaatc aacttggaag acaaagagaa aaaattgcga
     2341 gtcagaaaac cgttttgaga tatgaacagc atttgccttc acgccttagc gattttaaat
     2401 aatagccttg aaagtgatta tggaaaagcc ctctacgctc aatgcaaaat tgacgtagaa
     2461 actatttttg atcgattgaa aggtgtattt ggtatgtgaa gaacccatgt cagaggcaag
     2521 aacgttgcac ctactgacat tggaatgatg cttatgagca tgaatctaac caaattatcc
     2581 cttgaggcta gaagaaaagc ggaagctttt caacacaaag cagccaaaaa taaaaaacgc
     2641 gacgaagcaa tcacatttat gattattcat cgcgtttttt atatttagag ctagttattt
     2701 gctagaccca ctatttacag atgaaacgtt tatcaccttt ggttttgggc atttagtgga
     2761 attagcagaa ccaggtcatt atgacgaaaa gtggcaaaat tggaaacttg aatctttgcc
     2821 gatttttcct gatcgatacg attttgaagt tgcaaaagat aagggaaagc agtttaaaat
     2881 tgttgcagaa cttctcaaaa aggcaaatac aattattgtt gcaacagata gcgacagaga
     2941 aggtgaaaat atcgcctggt cgattatcca taatgcaaat gccttttcaa aagataaaac
     3001 atttaaaaga ctatggatca atagcttaga aaaagatgtg atccgaagcg gttttcaaaa
     3061 tttgcaacca ggaatgaatt actatccctt ttatcaagaa gcgcaaacac gccaaattgc
     3121 cgattggttg atcggcatga acgcaagccc tttgtatacg ttaaatttac aacagaaggg
     3181 cgtacaaggt acattttcac taggacgtgt tcaaacgccc accttatacc ttatttttca
     3241 gcgccaggaa gccatagaga attttaaaaa agaacctttt ttcgaggtgg aagctaagta
     3301 aaacaagggt catttaaggg cgttctaagc cccacacagc gttttaaaac ccaagaggag
     3361 ctttttagct tttgtttctt ctaaacaagc taaaataggc aatcaagagg ggataattgc
     3421 tgatgttcaa accaaagaga agaaaacgaa tagtccgagt tcgttttctt taagtagttt
     3481 gcaatcaaaa gtcaatcagc tttataaagc gacagcgagc caaactttaa aagctatgca
     3541 aggactgtat gaagcaaaat tattgagtta tccaagaaca gatacaccat ttattacaga
     3601 gaacgaattt gcttatttaa aagcgaattt tggcaaatat agcggttttt taggacttga
     3661 tcttgaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc aaattttgac
     3721 gaattagtga aactctatga aaaagacgta gtaaaacatg caacatccta ttctaatcag
     3781 ctggtaaagc ttaattaggt tatataagct tgaaaataaa gggtttgtcc aatatggaca
     3841 agcctttttc ttatgttttg ccacagattt gccacactta ttgtgtaaga gtatcaagga
     3901 taggtataat ttgatacttt gtttttttag tgatgtgcaa ataaatttct gttacacgac
     3961 tacctctaga atgccctaca cggtcttgaa ttgcttctag gggtattcct tactctgcta
     4021 agaaactaat atgcgtatga cggaatatat gggtcgtaat atgtttatta aaactaagtt
     4081 tattatcaat atcaattcct gctcgttttg gattaccttt tcgatttaat tttccatttt
     4141 caggtgagtt gaggaaattg ttaaggacac tataatgaag aggtgttcca ttatagtcgg
     4201 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
     4261 tcatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
     4321 attggacatt tcggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
     4381 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
     4441 caatattttg gagtttcgac tctgtaagtc aacagggcca ttagttagaa tacctattgg
     4501 gatgcggttt ttatgtagag ccagcagtag ttgatgtatc tcaggaatca tcgtaatctg
     4561 atcgagttca tattcatagt cagcttggaa gtctagagct tctgtcctag aaatactatc
     4621 gatgccaaat tcttccatag tcttctgaca gcgaaaaatt ctaagctgct caatactgat
     4681 gagttttttc gtgtatttag gaaaagcaac atcagaccaa tatctgaaac gtttgtaaat
     4741 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac gtttaaaggg
     4801 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
     4861 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
     4921 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
     4981 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
     5041 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
     5101 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
     5161 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     5221 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     5281 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccaggt ccaataaaaa
     5341 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     5401 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagcaatt
     5461 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     5521 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     5581 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     5641 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     5701 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     5761 aacagaggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     5821 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     5881 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     5941 ctatttttta aaaaaacaaa taaaaaaaac aaaaaataaa caaaaaaagt gttgcttttt
     6001 gtttgaatgc ttgatattat ataaacaaag aaatgatgaa aacgtttttt cgaacatttt
     6061 gcaaaatgtt ttctacttct acgtagcatt tctttctaaa atttaggagg tggtcaaaat
     6121 ggctattgtt gttggtgcag atctcaaagg tacgagatta aaagatgttg tgaaaaattt
     6181 tcttgtagaa gaaggttttg aggtaattga tgtgactaag gacggacaag attttgttga
     6241 tgttaccttg gcagttgcct ctgaagtaaa taaagatgag caaaacttag gtattgtaat
     6301 tgatgcttat ggagctggtc cattcatggt agcaactaaa attaaaggta tggttgcagc
     6361 agaagtttca gatgaacgct cagcttatat gacacgtgga cataacaatt cacgaatgat
     6421 tacggttggc gctgaaatcg tcggtgatga gcttgctaaa aacatcgcta aaggtttcgt
     6481 caatggtaaa tatgatggcg gacgtcatca aatccgagta gatatgctta acaaaatgtg
     6541 ttaagaaaag gagttaagga atgagaatcg caattggatg tgaccacatt gtcacagatg
     6601 aaaaaatggc agtatcagaa ttcttgaaat caaaaggaca tgaggtcctt gatttcggga
     6661 catatgacca cgcacgtact cactacccta tctatggtaa aaaagtcggt gaagcagtag
     6721 taagtggtca agcagacttg ggagtatgta tctgtggtac aggtgtcggc attaacaatg
     6781 cagtcaataa agttccaggt gttcgctcag cgttggttcg tgatatgaca tcagctatat
     6841 atgcaaaaga agagttaaat gcgaatgtta tcggtttcgg tggaaagatt actggtggtc
     6901 ttcttatgaa tgacatcatt gaagctttca ttgaagctga gtacaaacca acagaagaaa
     6961 ataaaaaatt gattgcaaaa atcgaacacg ttgaaacaca taatgcacat caagcagatg
     7021 aagatttctt tacagaattc cttgaaagat gggaccgtgg tgactaccac gattaaggta
     7081 taacaaatga ttctgacagt cacactaaat ccttcagtag acatctctta tcctctagaa
     7141 acattgaaaa ttgatacagt gaatagggtg aaagatgtta gtaagacagc tggtggaaaa
     7201 ggcttaaatg ttactagagt cttgtatgaa tctggtgata aagttactgc tacaggtttc
     7261 ttaggtggta aaattggtga atttattgaa agcgaattag aacaatctcc tgttagtcca
     7321 gctttttata aaatctcagg aaacactaga aactgtattg ctatcttaca cgagggtaac
     7381 caaacagaaa ttttggaaca aggaccaaca atttctcatg aagaagcaga aggctttctt
     7441 gaccactata gcaatcttat taaacaatca gaagttgtga caatttcagg aagtttacct
     7501 tcaggacttc ccaacgatta ttatgaaaaa cttatccaac ttgcttcgga tgaaggagta
     7561 gcagtcgttt tggattgttc aggtgcacct cttgaaacag ttctaaaatc gtcggctaaa
     7621 cctactgcca tcaaacctaa taatgaggaa ctttcacagt tgcttggaaa agaagtaaca
     7681 aaagatattg aagaactcaa agacgttctt aaagagtcac tttttagtgg tatcgaatgg
     7741 atagtggtct cattaggtcg aaacggtgcc tttgcaaaac atggtgatgt tttctataag
     7801 gtagatattc ctgatatccc agttgttaat ccagtcggat caggggactc aacggtagct
     7861 ggtattgcat cagctttaaa tagtaaaaaa agtgacgctg acttattaaa acatgcgatg
     7921 acattgggta tgttaaatgc tcaagaaaca atgacagggc atgtgaatat gactaattac
     7981 gaaacactaa actcacaaat tggagtaaaa gaggtataaa aatggtactt acagaacaga
     8041 aacgcaaatc tttggaaaaa atttcagata aaaacggtgt tatctcagct ttggcatttg
     8101 accaacgtgg tgctttgaaa cgtttgatgg cacagtatca agatacagaa ccaactgtag
     8161 ctcaaatgga agaactaaag gttttggttg ctgatgaact tacaaaatac gcttcttcaa
     8221 tgttgcttga ccctgaatat ggacttccag caacaaaagc attggataaa gaagcaggtc
     8281 ttcttcttgc ctatgaaaaa acaggttatg atacatcaag tacaaaacgt ttgcctgact
     8341 gcttggatgt ttggtctgca aaacgtatca aagagcaagg cgctgatgcc gttaagttct
     8401 tgctttacta tgatgtagac agctcagatg aacttaacca acaaaaacaa gcttatattg
     8461 aacgtgttgg ttctgaatgt gtcgctgaag atattccttt cttcttggaa atccttgctt
     8521 atgatgaaga aatctcagat gcaggttctg ttgaatatgc aaaagtgaaa cctcgcaaag
     8581 tcattgaagc gatgaaagtt ttctctgatc cacgctttaa cattgatgtt cttaaagtag
     8641 aagtaccagt taacgttaaa tatgtcgaag gttttgctga tggtgaagta gtttacagta
     8701 aagctgaagc tgctgacttc tttaaagcac aagaagaagc gactaacctt ccatacatct
     8761 acctaagtgc aggtgtatct gctaaattgt tccaagaaac attgcaattt gctcacgact
     8821 caggtgccaa gtttaatggt gtgctttgtg gacgtgctac ttgggcagga tctgtagaac
     8881 cttacatcaa agaaggtgaa aaagcagcac gtgaatggtt gcgtactact ggattcgaaa
     8941 atattgatga acttaacaaa gttcttgtta aaacagctag tccatggact gataaagtat
     9001 agtaaaaaca taaaacggag gatattgtta tgaacagaga agagatgact ctcttagggt
     9061 ttgaaattgt tgcttatgct ggagatgctc gctctaaact tttagaagcg cttaaagcgg
     9121 ctgaaaatgg tgatttcgct aaggcagata gtcttgtagt agaagcagga agctgtattg
     9181 cagaggctca caattctcag acagctatgt tggctcgaga agcttctggg gaggaacttc
     9241 catacagcgt taccatgatg catggtcagg accacttaat gactactgtc ttattaaaag
     9301 atgtgattca tcacctcatc gaactttata aaagaggagc aaaataatgc ataaactcat
     9361 tgaacttatt gagaaaggga aaccgttctt tgagaaaatc tctcgaaaca tctatcttcg
     9421 tgctattcgt gatggattta tcgctggtat gccagtcatc cttttctcat ctatctttat
     9481 ccttattgcc tatgtcccaa atgcttgggg attccactgg tcaaaagaca ttgagacctt
     9541 cttgatgact ccttatagct attcgatggg tatcctagca ttctttgttg gtggtactac
     9601 cgctaaagct ttgacggact caatgaaccg tgacctacct gcgaccaatc agattaactt
     9661 cttatctacg atgctagcgt ctatggtagg gttccttttg atggcagctg agcctgcaaa
     9721 agaaggaggc ttcttgacag ccttcacggg aacaaaaggt cttttgacag catttattgc
     9781 agcctttgtg acagttaatg tttacaaggt ctgtgtgaaa aataatgtta ctattcgtat
     9841 gcctgaagag gttccaccaa atatctctca agtatttaaa gacttgattc cattcactgt
     9901 agcggttgtt ctcctttatg gatttgaact cattgttaag ggaactcttg gtgtaaccgt
     9961 tgcagaatca atcggtaccc ttcttgctcc tctcttctca gctgcagatg gttaccttgg
    10021 aattacactt atctttggtg cttatgcctt cttctggttt gttggtatcc acggtccttc
    10081 aattgtcgaa ccagcaatcg ctgctatcac ttatgccaat atcgatgcca acttgcatct
    10141 tatccaagct ggacaacatg cagacaaagt tatcacttct ggtactcaaa tgtttattgt
    10201 taccatgggt ggaacaggag ctacattgat tgttccattc ttgttcatgt ggatttgtaa
    10261 atcagaacgt aaccgtgcca tcggacgtgc ctcagttgtt ccaacattct ttggggttaa
    10321 tgagccaatc ttatttggtg caccaatcgt attgaatccg attttctttg taccgttcat
    10381 tttcgctccg attgtcaacg tttggatctt taaattcttt gttgacacat tgaacatgaa
    10441 ctcattctct gccaaccttc catgggttac tcctggtccg ttgggaattg tactcggtac
    10501 taacttccaa gttctatcat ttatcttagc cggactcttg gtagttgttg atactatcat
    10561 ttattaccca tttgttaagg tatacgatga acaaattctt gaagaagaac gttctggtaa
    10621 aacaaacgat gctcttaaag aaaaagtagc tgcaaacttc aatactgcta aagcagatgc
    10681 cgttcttgga aaagcaggcg ttgctaaaga agatgtagca gcaaacaata atatcacaaa
    10741 agaaactaat gtccttgttc tttgtgcagg tggaggtaca agtggcttgc ttgccaatgc
    10801 cttgaacaaa gcagcagcag aatacaatgt tccagtcaaa gcggcagcgg gtagttacgg
    10861 tgctcaccgt gaaatgttgc cagagtttga cttggttatc ttggctccac aggtcgcttc
    10921 aaacttcgat gatatgaagg ctgaaacgga taaattgggc attaaacttg caaaaactga
    10981 aggagctcaa tatatcaaat tgacacgtga tggacaaggt gctcttgcat ttgttcaaca
    11041 acagtttgat taataaattt gtaaaagtta tttgagacga gattattctt tcaattcctt
    11101 ataatattgt taaccatatc attgcatttc atttccttgg tttccggaac tcagaatatc
    11161 gtctcaaatg ctttttttga aaggatttat agttatgact aaaacacttc ctaaagattt
    11221 tatttttggt ggtgctacag ctgcttatca agcagaaggt gcgactcaca cggatggtaa
    11281 aggcccagtt gcatgggata agtatcttga agacaattat tggtacacag cagagcctgc
    11341 aagtgatttt taccataaat atcctgttga tttagaatta gctgaaaaat atggcgttaa
    11401 tggtattcgt atttcaattg cctggtctcg tattttccca actggctatg gcgaagtaaa
    11461 tgaaaaaggt gttgaattct accataatct ttttgccgag tgccacaaac gtcatgttga
    11521 gccattcgtg actttgcacc actttgacac accagaagtt cttcactcag atggagattt
    11581 cttaaaccgc gaaaatatag aacactttgt agattatgcc gctttctgtt ttgaagagtt
    11641 cccagaagta aactactgga caactttcaa tgaaattggc ccaattggag atggtcaata
    11701 cttggttggt aaattccctc caggcattca atatgatctt gccaaagtct tccaatcaca
    11761 ccacaatatg atggtttctc atgcacgtgc tgtgaaattg tataaagata aaggttataa
    11821 aggcgaaatt ggtgtagtac atgcgttgcc aaccaaatac ccttatgatc cagaaaatcc
    11881 agcagatgtt cgtgccgctg agttagaaga tatcatccat aacaaattta tcttggatgc
    11941 aacctacctt ggacactatt cagataaaac gatggaaggg gtcaaccaca tcctagctga
    12001 aaatggtgga gaacttgatc ttcgtgatga agacttccaa gcacttgaag cagctaaaga
    12061 tttgaacgat ttccttggta tcaactacta catgagtgat tggatgcaag cctttgatgg
    12121 tgagactgaa atcattcaca atggtaaggg tgaaaaagga agttctaagt accagattaa
    12181 aggtgttggt cgccgaatcg caccagatta tgtgcctaga acggattggg actggattat
    12241 ttatcctgaa ggcttgtatg accaaatcat gcgagtgaaa aatgattatc caaactacaa
    12301 gaagatttac atcactgaaa acggtcttgg atacaaagat gagtttgtag ataataccgt
    12361 ttatgatgat ggtcgtattg attacgtgaa gaaacacttg gaagttttat cagacgcaat
    12421 tgcggatggt gcaaatgtta aaggttactt catctggtca cttatggatg tcttctcatg
    12481 gtcaaacgga tacgaaaaac gttatggact attctatgta gactttgaca cgcaagaacg
    12541 ctatcctaag aaatcagctc attggtataa gaaagtagca gaaactcaag tgatagagta
    12601 atattacttg aaaatgtaaa aaattgttat ccgatacttt agaaaaagta caacacttat
    12661 ttagaccagg ctttgaaaag gtatatattg ttagtttcga agattgtcct atgattcctg
    12721 agcttgaagc taccccgttg ctaaaatgtg gtaaatggta tgtttcaact ggaaaagagt
    12781 ggatttgcca ttctgattta gagttatcag catttgaatg ggaattttta caatcactcg
    12841 atgtagaaat acgtgagact attcacttcg aggtaaatta tttgcctttc caataataaa
    12901 aagaaaggaa ttagttgtga ctatcgaatt aaaaaacgag tatcttactg ttcagtttaa
    12961 aacacttggt ggtcaactga cttcgattaa agacaaggat gggatagagt atctctggca
    13021 agctgatcca aattattgga atggacaggc gcctattcta tttcctatct gtggtagttt
    13081 acgaaatgat tgggctattt atagacctca agaaagaccc ttttttacag gtcttattcg
    13141 tagacatggt tttgttcgaa aagaagaatt tattctggaa gaagtcaatg ataatagcgt
    13201 gacctttagt attaaaccaa atgctgagat gcttgataat tacctatatc agtttgaact
    13261 gagagttgtt tataccttga atggtaaatc aattagaacc gaatttcagg taacgaactt
    13321 ggaaatggaa aaaacaatgc cttactttat tggagcccat ccagcattca atagtccttt
    13381 agtagaaggt gaaaactatg aggattatta tattgaattt agtgaggttg agtcttgctc
    13441 gataccaaag agttttcctg agacaggatt attagatttg caagatcgga cacctttttt
    13501 ggaaaatcaa aaaattctcg atttggacta ctcacttttt tcccatgatg ccattacact
    13561 tgaccagctc aagtctcgta gcgtaacgct tcggtcaagg aagtctggta aaggtcttcg
    13621 agtagatttt gatgattttc cgaacttgat tctgtggtct acaactaata aaagtccatt
    13681 cattgctttg gaaccttgga gtggtctctc cacttcactt gaagaaggta atttcttaga
    13741 agataaaaga caggtaacga aaatttcacc tcaagaaact tctagaaaaa tctatgatat
    13801 tactatcctt aattaaaatt tgttattatt tgagtcttca ataagatttg aatactctta
    13861 ccttgtgatg atagattggt cttctccgtg ccagtcttac atcgcaaggt ttttaagttt
    13921 tttgtgggtc tatgtcaact gtagtgggtg acgaaaagct aatatctaaa gacagtctgg
    13981 tagtaaaaaa taagattatt tactacttct tgatttaaaa taatagtgga gccattaatt
    14041 tgtggcaaat ttaatttaat attttgcctc aaattagcca cactttttat agaactaaga
    14101 ggaaaaatga aattttcttc ctattaatat agttgtttga aatcttaaat atagcgt
//
LOCUS       DAYYOA010000066        13900 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000066 DAYYOA010000000
VERSION     DAYYOA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.066"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..232)
                     /locus_tag="ACHBKA_001786"
     CDS             complement(2..232)
                     /locus_tag="ACHBKA_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445467.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            308..380
                     /locus_tag="ACHBKA_001787"
     tRNA            308..380
                     /locus_tag="ACHBKA_001787"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:341..343,aa:Lys,seq:ctt)
     gene            542..763
                     /locus_tag="ACHBKA_001788"
     CDS             542..763
                     /locus_tag="ACHBKA_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445468.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1087..1332)
                     /locus_tag="ACHBKA_001789"
     CDS             complement(1087..1332)
                     /locus_tag="ACHBKA_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445469.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1326..1727)
                     /locus_tag="ACHBKA_001790"
     CDS             complement(1326..1727)
                     /locus_tag="ACHBKA_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9445470.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1899..3707)
                     /gene="uidA"
                     /locus_tag="ACHBKA_001791"
     CDS             complement(1899..3707)
                     /gene="uidA"
                     /locus_tag="ACHBKA_001791"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM9445471.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(3735..5501)
                     /locus_tag="ACHBKA_001792"
     CDS             complement(3735..5501)
                     /locus_tag="ACHBKA_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM9445472.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(5514..6308)
                     /locus_tag="ACHBKA_001793"
     CDS             complement(5514..6308)
                     /locus_tag="ACHBKA_001793"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9445473.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(6319..7695)
                     /locus_tag="ACHBKA_001794"
     CDS             complement(6319..7695)
                     /locus_tag="ACHBKA_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9445474.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(7824..8471)
                     /locus_tag="ACHBKA_001795"
     CDS             complement(7824..8471)
                     /locus_tag="ACHBKA_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM9445475.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(8555..9982)
                     /gene="uxaC"
                     /locus_tag="ACHBKA_001796"
     CDS             complement(8555..9982)
                     /gene="uxaC"
                     /locus_tag="ACHBKA_001796"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9445476.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(10056..11135)
                     /gene="uxuA"
                     /locus_tag="ACHBKA_001797"
     CDS             complement(10056..11135)
                     /gene="uxuA"
                     /locus_tag="ACHBKA_001797"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9445477.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(11138..12769)
                     /locus_tag="ACHBKA_001798"
     CDS             complement(11138..12769)
                     /locus_tag="ACHBKA_001798"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9445478.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            13086..13757
                     /locus_tag="ACHBKA_001799"
     CDS             13086..13757
                     /locus_tag="ACHBKA_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9445479.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT         4427 a         2701 c         1837 g         4935 t
ORIGIN      
        1 actattttat tatagcatga tttttacctt catcttgtat tttcaatata tactcaatga
       61 cagctttttt aggtattttg taccttgctc caatttttat attttctatt gatctatctt
      121 tcagcaaatt ataagcttta ttttttccaa tatttaacat tttagttaaa tcagtaattt
      181 tcaatatcga tggataactt tcaaatattt tttcataatc ttgaaaatcc ataaaattca
      241 ctctcaattc tgataataag tattgcactt ttttatttta ggtgttagaa taacaaagta
      301 ttaatttggg ctgttagctc agttggtaga gcagatgact cttaatcatc gggtcgcggg
      361 ttcgagcccc tcacagccca ttgggtgcca aacccttaaa tacagtgtaa acgttggtgc
      421 ttagcattta gaaaacgtat atactgtatt tttttgtttt caaaagagac tgtagtaccc
      481 ctttttttca ctaaaagccc actgtagtca ttgatatttt tgataagaaa ggaacataat
      541 tatgcgtaat gaaattgcta atataacaat aatccgagaa actttacaaa accatactgc
      601 aaatgagatc tctaaacata caggattaaa tttgagtact ataaaaaaac taaaaagtgg
      661 agaacgttca atagaaaaac taaatttaca tgatgctatt tgcctcactg aatttggtct
      721 taaaaataat agaaaaaatg ttgaaatcaa catatggaaa taattcttaa tcagctgctt
      781 ttgtcaaaag cacataggaa tttcacctct ctccaagttt acggcgagcc ttacggaagt
      841 atcattatcc caacttcaaa tcatcgcttt ttattgacta taaaaattat tttagaatgt
      901 ttatcgctcc ttctttacag taagaaatca tggcgcaact aaaatatgct ccttgaagtg
      961 ttaacgtact gatagaatgt atttaaattt caatgatcaa taggttttct gtttaaaaga
     1021 atctactaat cctttaacta ttagaagatt cttttaaaca gaataagtgg aaaggggact
     1081 ttttagttag ttaattttaa attttaagat cgcttctatc aatttaattt ctagctgtct
     1141 tagcatatac tcatctacat aggtaataaa ttgccccgcc tcattaaatc catttgtcaa
     1201 acataactta gctatataac tatgataatg tttgagtatc atcttgattg aaattggatc
     1261 ttcttctaga gcagattgga taatggatag aggaactaat ggatactgcc gttttttggg
     1321 gttgtctacc ataagtcata ccccattttt aattgttcaa ctgccttctt tcgttgatac
     1381 cagataccac ttgtactcat attgaggatt ttaccgattt cagtatcatt aaaacctgca
     1441 aaataataaa gtaggataac ctttcttctt atttccgata aatgatcaat ggctgccgat
     1501 aacttttgat ctgaaattaa tatttccatt ccaaataaga aatagacatc ttgttcatca
     1561 aaatgagttt gggagtactt accttcattt gaattcgtca atggttcctc attatctgtt
     1621 atgttaatgt ttcgttttcg aatagactta gtttcgtttc gtaatacttt tttacagtag
     1681 gcatcaaatt gatgttgtat agatttctca ttgtaatttt gttgcatgat aaatatgctc
     1741 cttttttccc ctttccacta atacaacaat cagaaagcgc ctttttttcg aaaaaactag
     1801 aaaaaaatat ccttctttaa acagaaaatc tttttataaa aaaaaggagt tggcaattaa
     1861 gcagccactc cttttttata ttccttataa aaggtaaatt attttttata actgaaattt
     1921 ggtattgatg accatcgttt ctttagatag tgagcaatca ttttaggctt tctatctcta
     1981 gtaaagattc ctttcttatt tccttgcact cttaaaatcc cttgacttgt ttcaaaatct
     2041 gcaaaattcc acacttgttc accaacaaaa ttgtcacatt tatctattac ttcgtgattt
     2101 gcttcgtaat aacccacttg atactcttct gtaaacattg ttggtactgt atcatgaagt
     2161 ccagctactg tatctgctcc atattctgta aacattattg gcgtatcggg acatctttcc
     2221 atccatccat ctagctcttt tttcaacaga tattttgcat gttttaggtc tccacctgaa
     2281 acataccaac cataatagcg atttaaacat aaaacatcga caatatctcc tactttacaa
     2341 atatctggtg ttgactttaa gtaagtaacg attgttacag gtctcttctg agggtcaagt
     2401 tctttagtca attcaactaa aggttcaaaa tattctttag cgccttcaga atccgaatct
     2461 ggttcatttg caatagacca cattacgaca ctcggatggt tcttgtcgcg ttctatcaat
     2521 tccttaatcg ccattttgtg agcattttct gtccgtattt ctttccaagt atcatgtttt
     2581 tctacgttgt catctaataa agtagccatg aaattcaaat gtaatcctac tgcaggtact
     2641 tcatcaataa ctacaatacc ttctcgatct gctaagcgca taatttcttc tgaataagga
     2701 taatgtgaag tcctaaatga attagcaccg atccatttca ttaaattaaa atctttgata
     2761 tttactgctt cattcaaacc acgaccatta atatacgtgt cctcatgctt cccaaatccc
     2821 ttaaagtaaa aaggtttatt attaatcaaa aacttcccat tcgaaacctg aatactacga
     2881 atgccaaatt cttctgtgta cgtatcaatc actttatttt cgttaattaa actaactttc
     2941 atactatata agtaagcatt tagaggctcc caaagtgttg ggttcttaag ttgaatcact
     3001 ccttctttcc ccttagcttc tgctataagt tgagcttctt cgtcatagat tgctattaga
     3061 atttctccgg tccattctgt atcgatatta aattctacat ttgcatctga atcaattatt
     3121 tttgtattaa tagtgacatc ctttatatag tctttcggag ttgtatacaa tttaacgggt
     3181 ctattaatgc ctgcataatt gaaaaagtca aaattaacca aattttcttt ttttatgtct
     3241 ccatcttcct gtatcgtttc tttcaaaatc cctacaggta aagtgctatc gtcaataaca
     3301 ttgtttacag ccactgtaat tctgttactt ccatctttta taaactgatt aatttctgat
     3361 tcaaatggcg taaaccctcc tttgtgttga ttaatcaatt ttccattcaa aaatactttt
     3421 gcttcatgtg ttgcagatcc aaaacgaaga actaatcttt cttctaataa ttttttagga
     3481 agaatcactt ctttttcata ccacacccat ccaacatgat ttctaatgtt ctctgtttca
     3541 aataaatcat tataagaaga aggaactgaa attggataac tctctttttc aaccattggt
     3601 tctaaagcaa gttcttcttg taagtcatca tttgtatcta acttaaatct ccaaagaccg
     3661 cttaaatcaa ttacttgcct cgtctctgtc ataatgggat ataacatgta aatcactcct
     3721 atataattta aatattaaat aaatgtatcc tttaacccac aataagcatc tattggactt
     3781 attcccttaa attcagaaag gcccattagt ttgtccacta atatttctaa agtttcttgt
     3841 tttccatcat aagtattgat ataagttttt acttgaggca catcagctaa atgaaacgga
     3901 cactgtacag atacaaaaat tgttggaatt tcatgaatat aaaatggaat atccggagta
     3961 cctttacttg ctggccaaac aattctttga actgtacctg ggttaacatt agctatatta
     4021 ataatcaagt catatttttc agttaaatct ttgattggtc tcttagcagc atatattcca
     4081 gctatatttt taattttttc ttcttgcgat aatcctttca attcttcttc aggtgaaatg
     4141 tatttttcaa cttcaaatcc ttttgctgac aaaatctgtt tcaataaatc aatggcctga
     4201 ggtctatttc cagcaactac ttcaccaaac ccacctttta ccccatgtac atctaccagt
     4261 aaaatccttt tatatttttc tggtacaata ggccaaatat cttgtttatc tttaactagt
     4321 gtaattgctt gatcagctac ctctttaaaa attgatttat tttcaattaa accaattttt
     4381 tccatagctt cttgtttcgg taacaaaatt ttattctttt cttttttatg caaacctagt
     4441 ttagccttta agcctaaaat tcttcttaat gcatcacgta aacgctcttc tgaaataatt
     4501 cctttgtaat aaccttccat catataatta aagtcttcat ctggatcatt aaaaaatagg
     4561 aatagatcac atccagccgc aatagaagtg ggcaataatt ctgaccgttt cattgcccca
     4621 gtcattccta acatatgact agcatcagta actactaatc cattaaaccc gagttttcct
     4681 ctaagtaagt cagtgattaa ttcttttgat aaagttgcag gaagtaaatc ttcatctttc
     4741 agatcaagat taaaatgttt tgaataagct ggcatatgga tatgacctgc cataattgaa
     4801 ggtaatccag cttctattaa gcctgagtat acttttccaa aagaattatc ccattcttct
     4861 gtacttaaac tattaatact aaaagataga tgttgatctc gttcgtcaat accgtcacca
     4921 gggaaatgtt tagctgcagg caaaattcca ctctcttgaa tacctttcat ataagctaaa
     4981 ctaagttcta aaactttatc agggctttgt ccaaaagatc gactagaaat gataggattt
     5041 ctccaatttc tattaatatc tacaatagga gcaaaactcc agttacatcc aatagccgat
     5101 gcttcaattc cagaaactct ccctaattca taagcatatt tcgcatcatt tgttgcacca
     5161 atttttactt ctacaccaac ttcggtacca tctgtacaag ctccgttacc acctgcttct
     5221 gtattagcag caattaataa aggaattttg ctttctgttt gtaggatata gttttgatca
     5281 tatacctcat cagcagttcc tggattatat cgtacagctc caatatggta ctgattgacc
     5341 atactactta agtaattttt atcacgacta gatcccatat taacaaataa ttgacctatt
     5401 ttttcttcta aagtcatagc atcaatcgtc tcttcaatcc aagaaatagc atcttcatct
     5461 aaatgatatg gtttttttcg taagtctact tttatagtca ttttctttct cctctactta
     5521 ttagtaagat ttttccaaaa agtatctata tatttttgtt caatatctat atactctccc
     5581 gataaacatt taaataaaat atcagttctt aagttttccc atgattcttt ttctttatta
     5641 accaaaatag gataaaagaa aactttattc ttatttgcag cttctagatc accaggagaa
     5701 tcaccaatca ttaatatctt atccaaatca caaccatttt caattacact tcgaataatg
     5761 tcttcttttt ttcctttatc ttgacaacac agttcatcaa ctaaggataa taattcatgt
     5821 ctttcccatt cttcttgaat agcttcctta ttagctgagc ttacaataat tatcttccca
     5881 tattttttca gagcttttat agtatctcta gcacttaaaa agggtttatc taatccttta
     5941 gcttgactaa tctgtcgatt gacttcttcc gaccatttta aagcttttaa taaatcctct
     6001 gactgagagt attctatttc cctcttcaaa gatggattag acaattcttt tgttacagat
     6061 acccagtgct ttaatcgatt aatcgatggt acatcatttt tttctaatgt tttaactaaa
     6121 cctaaaaatc tgtttattcc acgagtctct gaatataaat taatattatt ccaatcctgt
     6181 aggaattttt ccctatgatg aacatcaaaa atctctgcag caattggccc gaaaaagaga
     6241 ttgtgtttat aagtcattgt atccatgaca caaccatctg aatcaataca aaatatccaa
     6301 ctgttcatgt tttttctcct agttatgttt ttgatttagc aaattataat tttctaatac
     6361 tttttcttta gataatggat atacgaaaaa taaaatcaat cctacaacaa agaaacaaac
     6421 tgctggaatc aacgttgaag agttatacag cttttgtaaa acttctccac tttgtacttt
     6481 tgctaacgaa tcatacccta ttatagaaag agtccaacct ccgacaccgc cagctaaagc
     6541 ttgtccaatt tttcgggcaa aagaatatac tgcataaata gttccatctt ctctttggcc
     6601 agatttcact tcttgatcat cgattacatc agtaatattc gcccaaatga ctgtattaaa
     6661 aatacctaac cccatatatc caacagaagt aagaactaag aatacataca tattactagg
     6721 ctttatcaaa aataaaagta aatatacaat agcagagaag aacatcccta ctgacgccaa
     6781 ttctttttta ccgtattttt tactgaaaat taaacttaca ggtgaaacaa gtaccaacat
     6841 caaaatagga ttgataaagt tcattaaaac tattccctgt gcattattat aataatctgg
     6901 aaatacataa ttgttcatac tcattgttaa aagctgagca agcaacaaaa aaatagctgc
     6961 agctattata gaaagcaacg ctctattttt agctaatcct tttaaagatt tcaacaatga
     7021 aacttttaac tgcgattcat cacttttaac ccgttctaca gttaatttat aacatattac
     7081 gtaacaaata actgcacaca ctgaaaatac tcctgccaca attgtaaaag ttgatccacc
     7141 acgtacaatt tgagctccag aactatctct tgtataaata aaaattggtg ttaacacgcc
     7201 aatgaacata cctgctaagg tagctcctat actacgaaat gttgataacg ctgtcctttc
     7261 ttctgcaaca gaagtgatag ctgatgccat tgaaccgtaa ggaatattaa tagatgtata
     7321 aaaaatactt ccccatagta tatatgttac atacatatat ataactttaa tactcataga
     7381 agctgatgcc attgatgttt gatacattaa aaaacttgct aaagcgactg gaccactcat
     7441 ccgtaaaatc cacggtttaa atttgccagt tttagatggt tttgatccat ccacaatacg
     7501 ccccattgtt atgtctgtaa ctgcatcaat aaatcttgct actaaaaata atgttcctac
     7561 ggtagctccg cttatcccca atacttttgt ataaaaaatc attaaaaaag aactagcaaa
     7621 aatgaaagtg aaatcattcc caaaatctcc aaacatatat cctattttgt ctcttagacc
     7681 aaattttctt tccatataat ttgcgctcct tcctattgtt aagcgttttc attctgttga
     7741 actgccaact tacgtactat actctgaagt ataaagagtt agtatttatt ttttacattt
     7801 tatagtatta tttatttttt ctcctaataa ttctttatct cataaaactt atctatatat
     7861 ttactagcta ttcgagttac ttcttcaaaa tcaccttttt caacaggagc caacaaattc
     7921 ccacctacac caactgcaac aacgcctgct tcaaaccact cttctaagtt atctagactt
     7981 actccgccag taggcattat atttacatat ggtaatggag cttgatacgt ttttacaaca
     8041 gatggaccaa aagcagaacc tggaaatagt ttaatgattt ctactccagc ggtaagtgcc
     8101 tcctttatct cagtaattgt catacaacct ggtaaataag gaatttgata taaattacaa
     8161 atctctgctg tttgtcgatc aaaagatgga cttacgatat attctgctcc agccataatt
     8221 gcaatacgag cagtaaacgc atctaaaact gtaccggctc caattattac atctttttga
     8281 tcagcatatt ttttagcaat ttctttgatt atttcttcag cattcggaac tgtaaaagtt
     8341 agttcaatcc ctttaattcc acccgcagta attgcattta ttgctttaat ggcctcatct
     8401 ttattttttc cacgtacaac cgcaatgact ccagccgctt ctaacttact taaaatttct
     8461 acttttttca tatctatctc cttcttttat ggatacatac tctagctagt tgtactaact
     8521 tacaataaat aaatttaagc ttttattttt tgtattattt tctactttcg atttcaaaaa
     8581 aattaaaata attatacgca ttacgataac aaatattctc cactatctga ctaagataca
     8641 tttcatcatc tggaattcga cctgctttaa cccactcgct tagtaattga cataatactc
     8701 gacggaaata ctcatggcga ggatagctta gaaaacttcg agaatcagtc aacatacctg
     8761 taaaattcga caacaaactt tgttgcgcca tcgtagtcaa ctgttgacgc attccttcag
     8821 ctgtatcatt gaaccaccaa gcacatccca attggatttt ttgaatcata cccccttgga
     8881 agctttgcat tgctgttgct agtggaagcc aatcggttgg atttaatgaa tataaaattg
     8941 tttttggtaa gcacccactc ttttcagcag tgtcgaacaa tgctacaaca ttttgtgtca
     9001 aactagcttg atcccccatc gaatcaccac cgacatctac tccaatttgt tcctttaata
     9061 tacttgaatt atttcttaaa gcatttaaat gcatttgcat cgtccaacca tatcttgtat
     9121 actcagacat caaatgtaat tgaatacctg taacaaattg tgcaatttct gataaatgga
     9181 aatccgtttt tcctgctaaa gcttttatta acagattgtc gagtattttt tcatctgcta
     9241 tttcataaaa ataagtattc aaaccgtgat ctgctaatcg acagccaatt tgatgaaaat
     9301 aatccactcg ttggctcaat gcctgcttca acccattaaa gtttttgatt ggtatacccg
     9361 ctgcttgact aagttttaaa atatagttcg aaaatcctga tgatttgata ctccagacct
     9421 tatctggacg gaaagtaggt aataccttga aagaattatc ctgtttagaa agctcagtat
     9481 gatatcgcaa gtcatctgta ggatcatccg ttgtacaaat ggctttcaca tccatcatct
     9541 tcagcaattc ttttgggcga taggaatccg tttggatttt ttcgttggca cgtcgccaaa
     9601 tttctggtgc attcttctca gaaattgtca aatcaatatc aaagtaacgt tgtaattcca
     9661 aatgagacca ttcaaaaata ggattacctg gcgccttctc cactgttttt acaaaagcta
     9721 aaaatttttc ataatcatta cctgcaccac tgatcaactc ttcttctatc ccattagctc
     9781 gcatcaatcg ccatttataa tgatctccag ctcctttatc atttaaccat acctgagtca
     9841 aattcttgta ttgcttattt tctagcaaac atgctgggtc taaatgacaa tgataatcga
     9901 tgattggttg gtgttctgca aatttatgaa aaagtctttt agctatttca ttctctaata
     9961 aaaagttctt atctaaaaac atatttaagc actcctttcc ttcccttagt ttttagaaga
    10021 gaaaattttt cttctaaata gactccatta tataattatt tactcgcccc cttttcaagc
    10081 gcttcccaaa taccatttaa ataaactgca cctagagctc ggtcatataa cccataacca
    10141 ggtctaccgg tttctcccca aatcatccga ccatgatctg gacgaatata gccattaaat
    10201 ccattatcgt acattgtttt taatatctta tacatatcta gagaaccata ttctgattta
    10261 tgcggcgctt cataaaaatc gagtccatta tcacttaaat atttgatatt acgaacatgt
    10321 gcaaaaggaa tccgatcccg ttgacaaaat tcagatagaa tttcatagac atcattatct
    10381 ggtcgttctg caatacttcc agtacataac gtaataccat tagaaggaga atccactaca
    10441 ttacacaacc attctaaatc gtcacgattt ttaacaatac gaggtaatcc gaaaatagaa
    10501 tatggtggat catctggatg aactgccatt ttaatcccaa cttcttcaca tgtcgggata
    10561 attgctttga gaaaatatgc aaagttctct cgcaatttcg cttcatcaac ttctttatag
    10621 gcttctaata attgcttgat ttgtgataac cgatgaggtt cccaacccgg caattcaaaa
    10681 ttattagtag aattttctac ttttctaata atttcctctg gatcagtagg aatatttttt
    10741 gaaatatagg ctaacgtaga agacccatca tctaaatgat aatccaaatc tgattttacc
    10801 caatcaaaga ctggcataaa attataacag ataacctcaa tcccaaattc tgccaaattg
    10861 cgaatcgtct gtttataatt ctcgatgtat tgatctcgtg ttggcaaacc aattttgata
    10921 tcatcatgaa cattaacact ctcaataact ttcaaggtaa ggccattggc ttcgacttca
    10981 tcttttaaag ctttaattcg ttctctcggc cagacctctc cggctggtat atccattaat
    11041 gttccaacaa ttcctttcat tgctgggatc tgacgaattt cttgtagtga gattttttct
    11101 tcatttgccc cataccatct aaaagtcatt tccatagtta ctttcctcca acttctgtta
    11161 tatatttatt gattgtttta cgaactgctt gaggacctac tagcatttcg caaaagattt
    11221 tttcaatttt ctttcctatc tcaacttgat ataaatctaa accaaatatt tccttatttt
    11281 ctaagatttc ctgtaccgca caatgaatct ttgcaggatc ttgttcacct aatttcaata
    11341 ctgagaattt ttcctgcaac tctaccaata aaggatcagg acttggcaca aaattttttc
    11401 cttgatcatc gactgccaat aaataacgta accaagcagc aatagcgata ggaataaatg
    11461 ttagattttc tgcatttccg tctttttcga catacttttt aattgtttca ccatagcgaa
    11521 tcgctacttt ttgagaagta tctgttgcaa ttcgttgcgg tgtatctgga atatagggat
    11581 taggcaaacg tttattgatt acttcatcaa taaattgttt tggatcaata acgcctggac
    11641 tttcaactac tggtaagcct tctacataac ctatcccttt aattaagcca accaaatcat
    11701 catcttgcat ttctgcctga attgatttat accccaacaa acaacctgtt attgctaatg
    11761 ccgtatggag cggatttaaa catgtcgtaa ccttcatgat atctgcttta tcaactgttt
    11821 ctctatctgt cataatcaca ccagccgctt ccagtttagg tcgaccattt ggaaaactat
    11881 cttcaatgac tagataatgg actttttcag tattggtaaa agcagcgata ttggtatttt
    11941 tcgcagtatg aattatatct aagtcttcga aacccgcctt caataaatcc attttgacac
    12001 tttccgaagg atttggcgta attctatcaa tcatactcca aggaaaagtc actttttctt
    12061 tatcctttaa ataagcaata aatttttcag aaacgtatcc ttttttttgc caagcttctg
    12121 caatcataaa aatgctatct tgaaaacgtt gaccattctg tgagaaatta tcagtagaaa
    12181 ccatcgcaat cggtaactct ccagcattaa acctgctata caacaaggat gtcacaatac
    12241 tcatcgtatg ttgaggattg gtaggtccag attttatatc tgcttccact accgaaaaaa
    12301 attgtccttt agaatctttt aaactgtatc ctttttcagt aatagtaaac gttgtaaact
    12361 gtaatgtagg atcttcaaaa tatttaacca ctctttgata gtccgttata ttctttggat
    12421 tgcaatataa actatcagct gttactgcta aaagtctttt tttgagttca ccatcttcac
    12481 ccataacaat ctccaaaata tcattgttgt agtcatgata agctttttct actacttctt
    12541 catcaaatgt ctcacacaca actactccgg attctaattc attttgatct gctaagcatt
    12601 gagcaatttc tgcatgaaat cctcgataaa gatttcctcc accaaaatga agccacactg
    12661 gatgtttctg accttcttgt ttgaccgctt cgacattaaa atacggtagt tgaacttgca
    12721 ttttgttcaa tccttcgata gaatcttgcc agtctttaat caaaaccata tttacccctc
    12781 catactgata tgttagtttc cgttacaatt gtagtataaa atcgaatgta atcgctgtca
    12841 ataagaaaaa actaaaaata ttcttttttc taccatgtca gaattattgt gtcttttttt
    12901 agtaaaagca caccaatata tattttttag aactaactta aattaaagct taatttctct
    12961 ttgatcttat ttgagtattt tttgtttttt taatagctag tatgttactt tgaaatttga
    13021 tattatataa cgagcataga actaaaatat caaattcaaa aatgaaattt tgagagggat
    13081 taaatatgac aattaaaact agtctccaaa atcaagctta tcaatctatt cgtcagcaaa
    13141 ttatctacgc tgacttagaa cctggaaaaa aagtatcaga aaaaaaattg gaagaaacgc
    13201 tttctatcgg tcgtactcca atacgtgaag cattgattca actccgtcat caaggacttg
    13261 ttgatacaat tcctcaaagt ggaaattata tctcattaat cgacttagat ttagcaaaaa
    13321 atgctcgttt tgtcagagaa catttagaac gacaaattat gctagagtgt tgtgccaaga
    13381 tgaataatca aaagaaacaa gttttagaaa ctataattgc agaacaagaa aaagctgtta
    13441 gtcaaaagaa tgctcgtagt ttttttcata atgataattt atttcataaa gcttgttttg
    13501 aaattgcaga acgggaagaa gtatggcaat ggttggaaga taataatacg cacttggaac
    13561 gatttcgttg gttacgagta aaaacggaag gattaaaatg ggaaaaaatt atggaacaac
    13621 atcatcaact atttaaagcc ttgaccatgc aagaccctga agaagctaac ttcttaacat
    13681 ccttgcatct tcatttaatg cttgatgaac aaaatgttgt attgactact cacccaaaat
    13741 attttaaaaa tatataactg ctaaatttct tttttttaat ttatttacat ttctgattac
    13801 tacagaaaac taactacttt ttttatgtaa agttctgtca aatatagtca ctttatatta
    13861 ctttctcttg agtaaaaaaa catgtgaggt cgtaaacaaa
//
LOCUS       DAYYOA010000067        13900 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000067 DAYYOA010000000
VERSION     DAYYOA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.067"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACHBKA_001800"
     CDS             complement(<1..175)
                     /locus_tag="ACHBKA_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9445480.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
     gene            complement(654..1373)
                     /locus_tag="ACHBKA_001801"
     CDS             complement(654..1373)
                     /locus_tag="ACHBKA_001801"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9445481.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1422..3068)
                     /locus_tag="ACHBKA_001802"
     CDS             complement(1422..3068)
                     /locus_tag="ACHBKA_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM9445482.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3247..3837
                     /locus_tag="ACHBKA_001803"
     CDS             3247..3837
                     /locus_tag="ACHBKA_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9445483.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3929..5434
                     /locus_tag="ACHBKA_001804"
     CDS             3929..5434
                     /locus_tag="ACHBKA_001804"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM9445484.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5518..6870)
                     /gene="celB"
                     /locus_tag="ACHBKA_001805"
     CDS             complement(5518..6870)
                     /gene="celB"
                     /locus_tag="ACHBKA_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9445485.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6870..7388)
                     /locus_tag="ACHBKA_001806"
     CDS             complement(6870..7388)
                     /locus_tag="ACHBKA_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445486.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7404..7730)
                     /locus_tag="ACHBKA_001807"
     CDS             complement(7404..7730)
                     /locus_tag="ACHBKA_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM9445487.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7743..9722)
                     /locus_tag="ACHBKA_001808"
     CDS             complement(7743..9722)
                     /locus_tag="ACHBKA_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9445488.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9743..10057)
                     /locus_tag="ACHBKA_001809"
     CDS             complement(9743..10057)
                     /locus_tag="ACHBKA_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM9445489.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10225..10518
                     /locus_tag="ACHBKA_001810"
     CDS             10225..10518
                     /locus_tag="ACHBKA_001810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM9445490.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10596..11708)
                     /locus_tag="ACHBKA_001811"
     CDS             complement(10596..11708)
                     /locus_tag="ACHBKA_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9445491.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11861..12709
                     /locus_tag="ACHBKA_001812"
                     /pseudo
     CDS             11861..12709
                     /locus_tag="ACHBKA_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13062..>13900)
                     /locus_tag="ACHBKA_001813"
     CDS             complement(13062..>13900)
                     /locus_tag="ACHBKA_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9445492.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4336 a         2837 c         2381 g         4346 t
ORIGIN      
        1 tagccaagaa cccatctgct gaaatatgaa aatttttgaa tgctacgaca ttcgccatta
       61 caggtatgta aaagaaaaaa atcatccaaa gcatacccgg tacagccatc aggacaagtg
      121 ctttataacg ccatagattc ataaaaaaat gattgaaccc ctttcgtttt ttcatctaaa
      181 aacccctttc gattcattcg caacgtaatt atagcgcttt caataatgag aataaataaa
      241 caaagtatag ggaaaatgaa acaaagtata ggagaggttg tgaaatcagc gttttggcct
      301 gagtattaga gaaaaaaacg ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat
      361 accgagggtc agctgattga acaccgttta tcagaggttg tgaattcagc gttttggcct
      421 gagtattagg gagatcgtgt ccttactatc taactctgag tcacaaggaa caatggggac
      481 tgtaacaaaa gtcttgtcac agtcccttat tccgaacaaa cggtgttcca gaagtagctt
      541 ctacggaaat aagccgaaat ttcacaaaaa agccatccga aagtcagacg gacaagtctg
      601 cttttcagat ggcgctacaa ctactctgct tattctttta gctatccatt caattatttt
      661 tctctcactt gttctagctc ttctttggtc atgggtcgat attgcccttt attcaaatct
      721 ttaggcagcc aaaattttcc catacggatt ctttttaaat ataagacttc ttttcctact
      781 gctttgacca ttcgcttaac ttggtgaaac tttccttcat gcaaaatcag acggataatt
      841 gaagtttctg tctcttgatc tacttgatca atttggagtt ttgccggcaa tgtctgctca
      901 tcgccatcaa taatcagtcc ttctgcaaat aaacggatat cttcttctgt cattataccg
      961 ctaacctcag ctagatattc tttctccaca tgttttttag gtgatagtaa ctgatgggca
     1021 aacaccccgt cattcgttaa aatcaagagg ccctctgtgt ctttatctaa tcggccgact
     1081 ggaaaaagat cttcccgata atcctggtct tccaataaat cgattacggt ttgatcatga
     1141 ttatcctgag tagcagaaat gactcctgca ggtttatgca aaatgtagta aaaatctttt
     1201 tgatacgtaa tgatttctcc taaataagta accgcatctt cgaattcatt tacttgcgct
     1261 ttatcgcttt tcaccacttg tccattgact tcgatcgttc cttttttgat taatcctttg
     1321 acttctttgc ggctgccaag tcccatatct gctaaaaact tatctaaacg catgcgtttc
     1381 ccccatttca aaagagcatc aatctagctc ttctgattca cttattttat ttttaattta
     1441 tgtcgtaaac gcgctattcc tggtcctagt agtctttctg ccaagcggat tttcaaagct
     1501 aaataaccat acactaaagc ccctatacct gctactacaa gaatcaacaa tagagactgg
     1561 aatttgctat cttcgtttaa aaaagcacca aaaatcatct ttgtgatcca cgaagccaaa
     1621 agcataatca aggtaagaat aaagatcagc aatgtcctgc gccagacaaa acttggttta
     1681 aagcgggcta cttcatggat tctctttaag atcaaggtac aagacacagc aaatccgatc
     1741 attgtcgcca ataaagggcc gtatacttca aataaacgaa tggctgggta ttgaagaagg
     1801 agtttcacta gaaacccaat aaccaaataa ttaattgccg ccttgttttc aaacattcct
     1861 tgcagcatgt tggaaactag catatatagt cctaggaaga gtcctacaaa acttgcttgg
     1921 atcagtacat tacttcctag agaatccggt gtataaaata atgtatttag cggataggca
     1981 agtaacataa caccaaatgt cgccggaaac ataaaaaatg aaaacaactg taaatttccg
     2041 cttgtcaatt ttgccagacc tactcgatct ttcaacgtaa cagcttctgt aatcaacggt
     2101 aacccagtag acgcgatcga tgttgccaat gcaataacta ccatcgtcaa cttatctgga
     2161 ttggcactaa aaatcgaaaa aagatccagt aactgtgcat tggaatactc ggtggatgca
     2221 gacatgatct tcataaaagt gaactgatcg actaatttga agatcgttac ccctgaaccc
     2281 acaataataa acggaatcgc ttctttaacg gtatctaaca acaattcctt cgttgcaata
     2341 tttattttgt tttcgctgta gtgaataaac gccgatgtat acgcttgatg tttcttcaaa
     2401 aaatacaaaa ggacaagaat acttgctagc ataccgataa atgccgcgaa agttgattgc
     2461 gtgaccgccg tcacatagtc accatccatg actttcatga tgatgaatgt agccaatagc
     2521 atataaaaaa tacgtgccac ctgttctaca atctgcgaca gcgcataagg catcatttct
     2581 tgattgcctt gcaaataccc tcgtatcaca ctcatacaag gaaaaaccaa tacagcgata
     2641 ctcaaagaac gcatcgttgg aatcagttct tctccgccac cagaagcatg agctagccaa
     2701 ggagaggcga tgtacatgaa caaagcaaac aacaggccta acccgcccat catctgtaat
     2761 gcacggataa ataatcgtcg tgaagtgcca tattcattca atgaattata gcgtgcagtt
     2821 tgttttgcaa tcgcagcagg tattcctgct gtagagatca gtagaaacaa tgcatatata
     2881 ttgtatccca tattgaataa tccattggca gccttagcat tttcgcccat ccaagcatac
     2941 cagggaatga tatagacagc gcctagcaat cttgaaatga tattactggc ggtcatccaa
     3001 gcagagcctc gtgccatttt ttcttgcggt gttaattgat ctaatgctgg ttttctttga
     3061 ttatccatcg gtcagattcc aactttctac aaaacattac tatcatttta attggtaaaa
     3121 cagaaactgg caatcgattt tatataactt ttataagaac aattgttttt ttgaattcgg
     3181 tttcgttttt ttggtaaagt aaagaaatat acagcctttt atggctaaca tttataggag
     3241 atatcaatga cacaagaaat aaaaaaacta cccgttatat ttttaggtct tttctttatc
     3301 acagttggat tgaattggtt cttgctccct cacgatatag cttccgccgg agtgggcgcc
     3361 atcggacatt tagttgaaac ggccttttct atccaacgct cagttactgt ttggagcatc
     3421 aatcttatga tgctgcttct agccgctgtc ctattaggaa aatcagtatt ttttaagact
     3481 gtagcgggaa gtctgctttt tcctgttgtt ttagagattg ttccaaaagt ggaaatgatc
     3541 tcctcacatt tcttttcact gatttcagga agtcttttgt tcagtcttgg ggtttacaca
     3601 ttgtacacag tcggggcctc aaatggcggt ataacgatac cgccgattat tttccacaaa
     3661 ttttttcggc ttccaatgcc gcaaggactt ttacttacca atagtcttat cgtctttttg
     3721 aattattatg tttttggatt gttggagaca ctattcgttc tcctatctat tacagtcagt
     3781 tcattgttta tgaaaatact catccgcttg tcccctgtgt caagaaaatt tgaataaagc
     3841 tggattttgt catagcggaa acaaacgata tattaaaaag agtgtgctat aataaaccaa
     3901 gaaattgaat aaagcgaggg atcaatcaat gagcttctct ttagaagaca tccgtcattt
     3961 actattaaaa gaaaatctat taaaagaatt tgtttcaaca agaggctggc atcttgatgt
     4021 accagaaaaa gctgaatttc ttcagttaag ttatgattca agaaaagctg atgcttctac
     4081 tttgtttttc tgtaaaggaa tgaatttcaa agaagaatat ttggattcag caatcgaaca
     4141 gggtattcaa tattatgtat cagaacaacc atacgaaaat tcagcagtag gaatcatcgt
     4201 aacggatatc cgtaaagcaa tggcgcttct tgctatggct ttttacgact atccgcaaaa
     4261 taaattgaaa gtgatcggat tcactggaac aaaaggtaaa acaactgctg cgtactttac
     4321 aaaagcaatc ttggatcaca caacaaataa aaaaacagca ttgctttcta ctatgaatac
     4381 aacgttagat ggcaaaacat atttcaaatc ccatttgaca acgcctgagt cattagattt
     4441 atatcgtatg atgtcagaag cagttgaaaa tggtatgact catctagtga tggaagtttc
     4501 ttcacaagct tacaaaacac aacgcgtata cggcttgact ttagatgtcg gaatcttctt
     4561 gaatatctcg cctgaccata tcagtccgat cgaacatccg acatttgatg actatttcta
     4621 ttgcaagcga caattgatct tgaattcaaa aacagttgtt ttaaatcatg aaagtgacta
     4681 ttttgattta cttagggaaa cagccgatct attccagatt ccaacgatca cttatggtcg
     4741 ttcggaagaa agcaattatc aagtgatccg ttcagataaa ggtactcacg gttttacttt
     4801 atcttctcat gaagatcgct tagctatctg cgatactgct tatgatattt tgctggctgg
     4861 aggattcaac caagaaaatg cagccagtgc gatcattgcc gcagcactaa caggtgcctc
     4921 cagtcaagat gcacaagacg gattaaaaga agctcgtgta ccaggccgaa tggatcaatt
     4981 gatccaatcc aatggagcgc atgtctatgt ggactatgca cataattatc taagcttgaa
     5041 aacattgctg aaatttgcca aaaatgaaca tccagatggc cgtgtaattg ttgtattagg
     5101 cagcccagga aacaaagcaa tctcccgtcg acatgatttt ggaaaagtgc tatcagaaac
     5161 agctgacatc gcctttttga cggcagatga cccagcattt gaagatccga aaaaaatcgc
     5221 ggaagaaatc aatgaagcaa ttactaatcc agatttgatc gttcattatg aaatggatcg
     5281 tcctgaagcg attcgacgcg ctttagcaga aagcgctccg caagattcag tcgttattgc
     5341 gggaaaaggc gtggatccct atcaaaaaat caatggtgta gatgaacctt atgaagggga
     5401 ttatgcaatc gtgaagcggt tgattgaaga ataatggtat ttatatacta gtggtttccc
     5461 atttctaaga aaaaaaggga ctgtgatttt ttgtcacagt tccttttttc ttagaaatca
     5521 ttttaacttt tcactagcta attctgctgc cttttctctt tcttcattta ataatttatt
     5581 atcatagaat ttgataaatg ggaaccagat aatgaatgcg atgattgcac aaatgactgc
     5641 taacacagca gctttccaat caccaccact accaacaaat gctccgatcc cgactggtga
     5701 aggccacggc atctgtgcaa gcatcggttt gacgatttcc agccgaatgg caaaataagc
     5761 gagtgtagca gaagccatcg gagctaagaa aaacggaacc gctaaatagg gattgtagac
     5821 gattggcatc ccgaaaatga ttggttcatt gatattgaat aatcctggta caagtgaggc
     5881 ttttcctaac actttcagct gatcagattt cgcgaggaat gcaataaaga tgatcaatcc
     5941 taaagtcgca cctgatccac caacggttac ataagaattg ttgaattctc ctgccaacgg
     6001 ataatttgcg ccttgtgcgt tagctgccat attagctaat acgatcgggg tcaagaaaga
     6061 agtgatgata ttcgctccat ggatccctac gatccacaat gcatggatca agaaataaat
     6121 caccatgaca ccaagccatg tattcgtcaa ctgcgtaaca aacccaaatg ggatagcaat
     6181 cattttgaag atatccgttc ctagtgcaac taataatcca ttgatgatca tcaccacaaa
     6241 cgcaatgaca aatgttggga tcagagcagt gaaagaacga gaaactcctg gcggaacaac
     6301 atctggcatt tttatgaccc aatttctctt gacacacata caatacaatt cgaccgcaat
     6361 aatcgacata ataatagctg taaagattcc tgaagttcct aaacgttcga caaaagagcc
     6421 catcctaata ccatttacta cagtttctgt ctcattgata gaagcaatca agctgatttt
     6481 accatcagtc atgatcaatt cagggataca cataaagaat gcaaaaacag atagtaatgc
     6541 ccccgtgagc ggattaacat ttaatttttg ttcatctcgt tcaattgacg ttaattcata
     6601 agcaaacaca atggcaaagt aaatagataa aatccccatc gtcattgtat tgacgatcat
     6661 gtacaagtca ctaactttga aaaaagttgc attaaaaaat ccttctaaaa atgggaacgg
     6721 taatggcaaa atatttaaaa ccaaaaacat cgagcctaca atcgtgaaag gaatagaagc
     6781 catccccgct gccatgacag cccgaacgat tttaagttgc gcaattttcc ccataggtcc
     6841 catcaggtat ttctgcaaaa aatcaaacat tagttattct cctttcctaa atataacgct
     6901 ttttcatatt gtttaatacg gatatactgt tggtcttggt tgagtgaaat tttctttaaa
     6961 cgatactgta taaatgcact ggcaaaaata ccgcttacaa caagaatgaa aacaaataac
     7021 tgtcctccat tgttatttgc cataaaagga tacaagaatg taaatcctga ggtaaacaac
     7081 actggaatca ctaataaatt aaagacactc tggaacaaaa aataccactt tgtccatgga
     7141 acgttcgttt ggtgttttcg ataaagcttt acttgttctc ctactgcagg caaaatagct
     7201 aaaatcagaa tcatcggtaa actacttcct aaacttttag ctgatagaag cagaatcatc
     7261 cattgcatgt tcatgaaaaa gaaaaaggca gtcaggtaac gtacgattag ataacgattg
     7321 aaatacatgt ttttcaaact aagttgcttt ttttctaatt cgattttcct ttttactgta
     7381 tcctgcacaa tgatccttcc tttctattta tttttttgat acaaataatt catttcctgt
     7441 gcaacttcca ataatgtcat cgtcgtcatc agatgatctt gtgcatggac catgataatt
     7501 tccatttcta tttttgtccc gctggcatat tctttcagaa gttgagtttg tgactgatgc
     7561 gcatgaagaa tctcttcatt tgcttgtcgc aacttctctt ctacttcttc aaacatctct
     7621 ttacgcatcg agcgaaatgc ttcatgtatc agtgtccgtg catttccgct atggaaaatg
     7681 atatcaaaag aagtcacttg tagtgtttca ctgtttattt tttcagccat ctgttttcct
     7741 ccttattcga tcagtttgat aaatttactg ctgaaattct caaaaatagg ttcagctaag
     7801 atttcctgct gtacttttag ctggtcaact aacctaacga ttgctttagt cacgaccgta
     7861 attccttcat tttcgacata agaaggcgaa actaaaaaaa caaaatgaat ttcttgatat
     7921 tgatcccact gcatcccaga aggaatcaat gcaacggcta cctttgtttc gattccaact
     7981 ggaacagcgg gatgtgggac tgccactgta tcactgaata tgatttgtcc catattttca
     8041 cgttgctgga tctgctttcg catttctctt atatatcctt cttcttcatt tatgctcagc
     8101 ttttgcagca aatcatgaag aatttcttcc tttttcggtt cgtacgtata agtcttgaaa
     8161 aatttttcag acaactgttc ggtgtaaatc tgcttttttt gctgtagaga aaatgctggt
     8221 atcggtctat ctgaggaatg atagggtata gattctccaa ctacctttcg tattttacga
     8281 acatcatctt cattcaaaaa aatactgaca tgtaaaactg gaattttgaa aaacattgtc
     8341 gaaagatcaa tagaagaaat aatcaggtcg attcctttta aagttgtttc attgatctca
     8401 taatagcctt ttacacttac cactgtaata tttttaccga attcactgac tacgcgattc
     8461 tttaatagtt gggcactgcc gtaacccgtt gcacagataa tcaatgcatg tagtttatgc
     8521 gcagctcttt ctttttccaa tgccgccatg agatgcaaag ccaaataggc ccattcatct
     8581 tcattgatgg tatagttttt caaagccggc atgttgctga aatgcctttt tactgcgtca
     8641 aagactttgg gattttcttt ctttatctcc tcagtcaatg ggttctcaat gcgaatacct
     8701 cggctcaatc gaatcatcat cggtgacaaa tgatcgagta gtccattgaa aagctggaga
     8761 tcctctacta atgaaaaatg aaattcctga gacaatcttt tcagaacttt cgctaattcc
     8821 tttgatagtt cctgatcatt ttgattttct ttgtgattcg acttagccat gagatgtaat
     8881 gtcaaataag caatttcttc ttcgggaaac gatacacctg cgattacctc aatacgttga
     8941 acgatccgct gcgccacttg atactctact cgcttagaaa cttcttgttc gatgcctaat
     9001 tccttgattt caaacccttc tcggagccgt ttgatcccca aagccaagtg taaagtcaga
     9061 ttctgtatga taaaatccga aacttttaac ttagcttctc ttgtttcatc gagaatgata
     9121 atgactagct cttcaaaact gatgtctggg aaaaaacggc tttgccctaa atattctttc
     9181 aatgaatttc cataatggtt tccaaaaaaa gtatccatga taaaatgccg tcgatttctt
     9241 tcttcccctt cgatccatgt cccttgacca tgcttagaaa cgatttttag tgaatatgga
     9301 atcaatcgct ccttgatttc ttgcatgtcc tttgtcaaac tggaacgact gatataaagc
     9361 tcttctgcca aatcatccat aaaaagaatg gcatcttcca aaagcaattg gttcaatata
     9421 tacttttgtc gatcttcgga ctcataaaat ccttgcggtt ctctagcata tccaggtact
     9481 atttcttgcc gactcaaaaa caagtcaaat gtcagtcttt gtaaaatatg cagttggtag
     9541 ccataacctg gtttagcaat gatcttacct ccgttttttt ctactaactc tttgatccgc
     9601 tgcaagtaat tacggattgt ccgatcagat aataacaatt cagaagctaa acgttgactg
     9661 gtgacatact ccttcttgtg atcaatcaaa tatctcaaca gcaatttttc cttctgtttc
     9721 attatcatcc actcctattc catcatatgt tctcttcgat cacctctgct aacttctgga
     9781 tgcctgtagg gattgggaca tacgcttgga aagggatact aacaacaggc tttccaacag
     9841 attctcctaa ttttttgaat ttatccaaaa acatcgtcgt ttgcgggctg atcagaaata
     9901 aatcaaaatc actattctta atcatattat cgccttctgt agctgaaact gcatctacga
     9961 gtacctcttc tcttttttgc ttaaagaaat ccgttgtctt tgaggccatc atcgaggatg
    10021 acattcctgc cgcacaaata atcaatgctt ttttcataaa tcaatcactc cttttatttt
    10081 tataaaccaa gcatatcttg taagcgtttt ctattcaaaa tatatatttc cgtagcattg
    10141 cggaaaaaat gtgtatcata tcttgaataa taatttctgg caccgtgata tactagtgat
    10201 agaatttttt caggagggga tttagtgagt atcacagtga atattttgta tacaagtaca
    10261 aatggacaag ccagaaaatt tgcagaagaa atggtcgaaa gaaacattgt caaagcgatt
    10321 cgtgcagaag aaggaaacga gaaatatgac tattactttc cactagaaga tccagaatct
    10381 cttttactga tcgaccgttg gaaagatgaa gaagcaatcg aaaagcatca caaatccgaa
    10441 atgatggctc aaataaacga actccgtaaa aagtataaat taaaaatgaa agtcgaacgt
    10501 ttcactgatt taccgtaaaa agatacacat ggaatgatag caatttatca tccatgtgta
    10561 tcttcacttt ttccttattt tttattcgat ctcatctaat gattcaaacc gtgtttttct
    10621 cttatttttc ggataagtat ttcagtcacc ccaatataca tgattcctaa acatacccca
    10681 aggatattct caaaaatgcg gatcgttact gctcctggaa ccccgaataa actggcagtg
    10741 attgccaaag caccaaaaga attaaaaact gttttatatc tataggtaga acaaacacct
    10801 aaagctagac cacctaatat accgacccag ttaaaaggaa taaattggct gataccaata
    10861 aataaaacac atccaatcaa tgagccaatg attcggtcta ctgctcgttc tttgaaacca
    10921 atcgacatca atccatagct agatacaata gaagaaaatg caaaccctgc ccacatcaat
    10981 cgctgaaaag ggacatactc tcctgcaacg atcaaaagac taatgccaaa tgcatagatg
    11041 agcatccaaa ttttttcctg cgaatacatg cctttcaaaa aaatctcttt aaacaagctt
    11101 ttgtctttgt tcttttctcg atgttttctg taaaggagaa ctgaaaacca acaaaaaaac
    11161 aaaaataata agctacttgt ttgtgtaaag aaattcttgt tcaacgaatc tttaggtaac
    11221 gaataaacaa taaagagata tgaaaagccg tacaaagaag cattccccat tttgggatca
    11281 ctagcggttg ccattaatat gctgcttagt aaaataaaat gaatcaacat gcccataact
    11341 gaccatttga tcagctggac atacggtgct actaatagtg agaacatgac gattgctaaa
    11401 cctatgatcg aatgggaaat cttataacca aagtcaacaa aacgaatact catcaacata
    11461 cagaataaaa ccacagcgta tggtttatgc ttcgattcaa ataatgttgt aattataatg
    11521 ataaaacaaa tggcaaacaa tacgatgaat aatgatcgta aaaacaatga acaaatatac
    11581 atgcactttt cttttttaga agctgcttct cgtatttttt gttttaaaat aaacggatct
    11641 agttgtagag actgataaaa atccacctgt tgtcgtcctt tctttttact tttatttttt
    11701 acagtcatct atgacaggtt gtaaatacta tggaacatca tatggcgttg tcattttctt
    11761 gtcaactatt tttcctattc atgtttttat aaaaaaacat atcgttttct tgtatgatta
    11821 aatcaccaca aaataaacaa ctgagagaac gatatgcttt atgaatggtt ctatcaaaaa
    11881 aattctgaga tcaatagaca aaaatttgat gattacagag gtctcttacg aaaccttaca
    11941 gaagaaaaaa acattgatcg ttgctgcagt tctcttccct gctccatgtg tctgtagaaa
    12001 ttgtggttct gctgtggtag atggaaatag gaaattaatg gtggtgaaaa acaagaagaa
    12061 ggaaaccatt gtccactttg aataatataa tcatatgcca atggttatac gcctaaaaaa
    12121 gcagcgctat acctgtaaaa actgtcgaag ccattggaac gctcaaagtt attttatccg
    12181 tccaaggcac tcgatttcaa atcatgtcag acataaaatc acttctttac tgactgaaaa
    12241 agtgtcgtta ttttttattt cgaaaagttg ttaggtatct ttgacaaccg ttattcgcac
    12301 attgaaagag ttgagaagct atttaccaaa gtcttccaaa agaattctgt cttgagtgtt
    12361 gagggttgtg aatttcgttc tcacgtttcc gttgaggata aactgagttt tatttccgcg
    12421 gatggcgaga cagaaaaatt aatcgagaaa atttacctac acaaaaaatt cctcgattaa
    12481 caaactattt ccttagctgc actaatccta aagaaataga attcttagtt acagacatga
    12541 acgccaccta ctttcagctc accaaacatg ttctaccaaa tgcgaaattg gtgattgatc
    12601 gattacttgg tttttccacg cctttcaagg aggcgtaacc tttattcacg agttggttga
    12661 agctttccga gacaaaagac ccgacttgtt tttctcctta ttgacaaaga gccattaaaa
    12721 ctagagaaaa aattacaact gatcgctgat tatttgtaga aagaaaatca aagtattacc
    12781 caataaactt gagtcattag gcaaaaaaca ctgcgaaact caagaatgat tttcgcagtg
    12841 tttttgtata ttttttatta actagtatta ctacaaaggt ttatttttca cttctcaacg
    12901 cagcacaaaa ttgtctttac ttatttactg gttgggttca accttctcta ctccgatact
    12961 aatatttgtt tgtatacccc ttctaaaatc ggtagtttcc attcaattga tgggtactag
    13021 ttgattttaa aaactatgct tattcaattt tttattttag ctcatatatt tcgtttcttt
    13081 ctaaatttta tactgatgaa aacaagtagc acacccaata ttgacaatat aaactggcta
    13141 atgatggttt ctccagtttt tggaagatgc ttatttccac tgcttgttgg attgtttcct
    13201 cttatatctg agctatcagc aacttttcta ttctcttttg ttaaaaagat cgccacttct
    13261 tgttcttttt cgatcttgaa agttactggt gtcgcatcca attcataccc cgttggcgct
    13321 tgagtttcta ccagctgata tgctcctggt gctaagccat ctatcgccag ttttccatct
    13381 gctcctgtgg ttagacctga ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca
    13441 gctccttgca ggacttctcc gctttggtca tcgatctttg tcaacacgac accgcctggt
    13501 gtaagtcgat tttctttcgt caattctacc accgctgtct gacttctttc gatctcaaac
    13561 tcgattggtg tcgcatccaa ttcatacccc gttggcgctt gggtttctac cagctgatac
    13621 actcctggtg ctaagccgtc aatcgccagt tttccatctg ctcctgtggt tagacctgac
    13681 tgcagaacgg ttccattggc atcttgtaac tcaaaaacag ctccttgcag gacttccccg
    13741 ctttggtcat cgatctttgt caacacgaca ccgcctggtg taagtcggtt ttctttcgtc
    13801 aattctacca ccgctgtctg acttctttcg atctcaaact cgatcggtgt cgcatctaat
    13861 tcataccccg ttggcgcttg agtttctacc agctgatatg
//
LOCUS       DAYYOA010000068        13810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000068 DAYYOA010000000
VERSION     DAYYOA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.068"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACHBKA_001814"
     CDS             complement(221..1423)
                     /locus_tag="ACHBKA_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9445493.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACHBKA_001815"
     CDS             complement(1426..1620)
                     /locus_tag="ACHBKA_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9445494.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACHBKA_001816"
     CDS             complement(1667..2515)
                     /locus_tag="ACHBKA_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM9445495.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACHBKA_001817"
     CDS             complement(2720..3070)
                     /locus_tag="ACHBKA_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445496.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACHBKA_001818"
     CDS             3248..4111
                     /locus_tag="ACHBKA_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9445497.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACHBKA_001819"
     CDS             4735..5727
                     /locus_tag="ACHBKA_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9445498.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACHBKA_001820"
     CDS             5743..6627
                     /locus_tag="ACHBKA_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445499.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACHBKA_001821"
     CDS             6676..8283
                     /locus_tag="ACHBKA_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9445500.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACHBKA_001822"
     CDS             8284..9768
                     /locus_tag="ACHBKA_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM9445501.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACHBKA_001823"
     CDS             9945..10937
                     /locus_tag="ACHBKA_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9445502.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACHBKA_001824"
     CDS             10989..11858
                     /locus_tag="ACHBKA_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9445503.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHBKA_001825"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHBKA_001825"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9445504.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAYYOA010000069        13790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000069 DAYYOA010000000
VERSION     DAYYOA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.069"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..876)
                     /locus_tag="ACHBKA_001826"
     CDS             complement(16..876)
                     /locus_tag="ACHBKA_001826"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM9445505.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            968..1396
                     /locus_tag="ACHBKA_001827"
     CDS             968..1396
                     /locus_tag="ACHBKA_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9445506.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1558..1734
                     /gene="rpsU"
                     /locus_tag="ACHBKA_001828"
     CDS             1558..1734
                     /gene="rpsU"
                     /locus_tag="ACHBKA_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM9445507.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            1761..2207
                     /locus_tag="ACHBKA_001829"
     CDS             1761..2207
                     /locus_tag="ACHBKA_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM9445508.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2361..3740)
                     /gene="brnQ"
                     /locus_tag="ACHBKA_001830"
     CDS             complement(2361..3740)
                     /gene="brnQ"
                     /locus_tag="ACHBKA_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9445509.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            4446..5417
                     /locus_tag="ACHBKA_001831"
     CDS             4446..5417
                     /locus_tag="ACHBKA_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM9445510.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            5441..7633
                     /locus_tag="ACHBKA_001832"
     CDS             5441..7633
                     /locus_tag="ACHBKA_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM9445511.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            7650..8126
                     /gene="ybeY"
                     /locus_tag="ACHBKA_001833"
     CDS             7650..8126
                     /gene="ybeY"
                     /locus_tag="ACHBKA_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM9445512.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            8110..8505
                     /locus_tag="ACHBKA_001834"
     CDS             8110..8505
                     /locus_tag="ACHBKA_001834"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9445513.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            8520..9419
                     /gene="era"
                     /locus_tag="ACHBKA_001835"
     CDS             8520..9419
                     /gene="era"
                     /locus_tag="ACHBKA_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM9445514.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            9562..10374
                     /gene="recO"
                     /locus_tag="ACHBKA_001836"
     CDS             9562..10374
                     /gene="recO"
                     /locus_tag="ACHBKA_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM9445515.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            10758..11675
                     /gene="glyQ"
                     /locus_tag="ACHBKA_001837"
     CDS             10758..11675
                     /gene="glyQ"
                     /locus_tag="ACHBKA_001837"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM9445516.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            11677..13752
                     /gene="glyS"
                     /locus_tag="ACHBKA_001838"
     CDS             11677..13752
                     /gene="glyS"
                     /locus_tag="ACHBKA_001838"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM9445517.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         4645 a         2478 c         2891 g         3776 t
ORIGIN      
        1 aaaagacaaa aatgattaat cgcttttttc tgttgtttca gttccataat aactatgatc
       61 ttctaggtct aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac
      121 attgatcact acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct
      181 aattttttcg atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc
      241 tttacggata ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc
      301 gatttcccac agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt
      361 taaattttta tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag
      421 caaaacgaca tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc
      481 aaattccatt gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg
      541 ttgccgaatt ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac
      601 atcgaaacta aataattgat gttcttcaca aaaagaacgt gttatctcga tcagttcttg
      661 attgattaat gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct
      721 taatgttttt tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata
      781 ctgagccata gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac
      841 aaaaaaagta acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt
      901 tttatgttta ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga
      961 ataagtcatg aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg
     1021 ttttaaatat actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta
     1081 cgtatctgct aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga
     1141 tacgatttac cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt
     1201 aaatggtgaa atgaagtttc gttttcattg ctgtcagggg gaaatcggcc atcaccatca
     1261 ttttatctgt acagtgtgcg gaacaacgaa agaaatccat atgtgtccaa tgaattattt
     1321 cgaagaacag ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg
     1381 tgaaaattgt caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag
     1441 atttccttga cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg
     1501 agatatattt caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg
     1561 tcaaaaacag tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt
     1621 tccgtgtcaa aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca
     1681 agtgtaagac gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt
     1741 gatgaattgg agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag
     1801 cgatgaaagc gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac
     1861 aaaacgagca gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt
     1921 ctcgcgaaat gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg
     1981 atttagctga aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac
     2041 tttccgacga agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat
     2101 caatgaaaga atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag
     2161 atggaaacca agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa
     2221 actgcggaca gtgattgtcc gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc
     2281 tagaagaaag aataagattc tttcttctag ctgttttttt cctgtttaat atctcttatg
     2341 ttaaattgtg tttgatttgg ttattcttct ggtagttcat ttgcttcttg tttataccct
     2401 gataggcgat aggcgtaata aaagagtaag ctgccagcgt aaccaagtaa cgttgggacc
     2461 atccagccaa gtcctaatgc agcaaaagga acgtgatgat gataaaatcc tagtaagctt
     2521 gctacgacag tttgctgact cactaatgca ggcgaatttg ccaacatatc caatacggct
     2581 ggaacggcag aaaagaacat cgttacttga tagacaaatg gactcttttg gaagaatttt
     2641 gctgttaagg aaagcaagat taacgcaagt gacaatggat acaaaagcat caatactggt
     2701 aaagaccatt ggatgatatt atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc
     2761 gttaaccgca gccatgtcat ataactgact ttagggaaca gtttatggaa atcttgtgca
     2821 aaagaaacaa cgagtcccat agcggtagtg aagacaccta atgtgaccat tacccctaag
     2881 aagctagcac cgaagttacc taaataaaaa ttagtgatct gagaaagtgc agttccgcca
     2941 ttttctgata aaggcatctt attcaaactc atcgcaccga gaaggaccag accaaaataa
     3001 atcatgactt ctaacgcaac gctcaggcta ccagcttttg ctgttaattc tgtgactttt
     3061 ttatctttaa aaccaagatc acgaactgca tggacaaaag tgacacttaa agcaagtaaa
     3121 gctactgcat caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc gttcgtctga
     3181 taagaatctg cagcagattg gtttagattc cccatgggtt ggaataaaga aacgacaaaa
     3241 atgatagcta ataatgcaag aaacaaggag tttaaatact ttccgacata tttgaccaaa
     3301 cgtgttggat gtacagtaag gaaataagca agtaaaaaga aaagagcaga gaataaaaac
     3361 atacctaatg aatcatattt ttcaggcaaa aagggtttag cagccatttc aaaagcagtt
     3421 gctgctgtcc gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag gatcaagaac
     3481 aaggcagcat accattttcc gacaggttta gctaagtcat acagaccatc actttttgtc
     3541 actactacag ctaagatagc taataatggg aaaagagcgc ctgagatggc aaatcctaca
     3601 ccagcgctga tccagtttga tccagccatt tgacctaagt ggacaggaaa gatcagatta
     3661 cctgacccga agaacatccc gaagataagt gaactgacca tcaataattg cttccatgaa
     3721 taagtttttg tttcttccat aaatttttcc tccaataatt atcattttga gtaacaaaat
     3781 aaacgttgca gaaactagaa atgccactaa tatcagattc catgaaaacc accaccttaa
     3841 aatataaaaa aaagcccttt tccacttggg aaaagggctc tagattctag aacggtacca
     3901 ccttttttcg cttaaaaaag cttcttcatt cacggacgct tttcgataaa gttatccgct
     3961 agcctgataa tggaggcagc cacaatgatc tacgttttcg acattgactc ggaaatgatg
     4021 ttcgtctatt cgatttattg tcatttctca cttacatgac tcactgggaa taaatcgctg
     4081 aatagcctac tgttttcctc gacgctttgc tgtatattta tttgttgacg ttataataca
     4141 cctgtttttt ttgaaatgca agaatatttc aaatattttg ttttcttttt ttgaaacaag
     4201 aaaaataccc agagaatact tgctaagtta gacggaatac ttgtcctcta tcttatgcat
     4261 agcttacatg atcagctttt tttattcaaa cgagaccttt agagaatgga agcagctatt
     4321 ttttaacgtt tcttggtact tatggttaag caactctttt agaacctttt cttatttgct
     4381 atatagtttc ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc
     4441 tatttttgac ggaagagtct agttctttag agattagatt gacggctgct gatgatgtca
     4501 gcatgctttt aggtgcacac gacaagcata taaaactgat tgaagaaaca acagaaacaa
     4561 ccatccatac acgtggagaa atcattcaga tcattggcga acaatcccag atttccttgg
     4621 ctgcctcagt cattcggaca ttgcaggaat tgatcaaacg aggaatccat gtaagtacac
     4681 cagatgtcgt gacggcactg aaaatggggc aaaaaggcac attagattat tttgttgaaa
     4741 tgtatgagga agagatcgtc aaagaccgta atggaaaacc aatacgggta aaaaacagcg
     4801 gccaaaagaa atatgtcgaa tcggtagcca aacatgatat cgtatttggc gtaggaccag
     4861 ccgggacagg aaaaactttt cttgcagtag tattagcaat cgctgccttg aaaaaaggtg
     4921 aagtgcaaaa aatcattttg acgcggcctg ctgtggaagc aggggaaaat ctaggctttc
     4981 ttcccgggga tctaaaagaa aaagtagatc cttatctgcg tcctgtttat gatgctttat
     5041 accaaatttt tggattagat catacgaacc gattgatgga acgaggcgtg atcgaaattg
     5101 ctcctttagc ttacatgcgg gggcggacat tggatgatgc attcgttatt ttggatgaag
     5161 cacagaacac gacagttgct cagatgaaaa tgttcctgac acgtcttggt tatcaatcaa
     5221 aaatgattgt caatggagat acgagtcaga ttgatctgcc tcgagggaca acaagtggtc
     5281 ttgttcatgc agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag
     5341 caagtgacgt tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcag
     5401 atctccatca agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca
     5461 gaaactacaa gaaaaacttg gaaaggcagc attgcctttc ctgctgttct tgttttttat
     5521 cattatctgc ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg
     5581 ccaagtagca gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga
     5641 acaaaaaaga aaactggcag cagaagcagt cactccagaa tacacttacc aaaaagatct
     5701 tgcagatgac cagaacaatc ggatcaagca actatttgaa ttgatcgata aaacgaatga
     5761 tgccatcaac aaatcttatg acgaaaagat tgataaggca aaggaagatg aaaatatccc
     5821 aaagcctact acagaagagc ggatcgccgc gttgaagaaa aattttgaaa atgtgaatgc
     5881 agaaaatgtt gcgttttatc aaaaactacc gaacaatttt tattcgacga tatttgaaat
     5941 gactgaaact gagatccata ctgtgaggga tgagagtctg aagctcattg acgaacagat
     6001 gagcaaacaa gttcgtgaaa gtgaattgga agcttttaaa caagaagcag aagagcaaat
     6061 ccaatatttg aatgtcacac cagcacaaca acaaatgatt cgctacttag tggatcaagg
     6121 tatcgtagtg aatgacgtat tgaatgagaa aaagacagaa gaattgaaac aatccgcacg
     6181 agaagctgta caaccagtga tgatcttcca aggagaaatc attgttcgtg aaggtaatca
     6241 aatcgatgct tcagctatga aaaaattgga attactagga ctgactaatc aaacaacttc
     6301 gatttttccg ctaattgcca tggttttagc tgttttgctt caaatcggcg tactgattta
     6361 taattcactt caattccaaa atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac
     6421 agctatgtca gtcagcatag tgctgatgaa atttttccaa ttattccaaa cggaacaagc
     6481 tacttatatc ccgctgtttt atcccgctgc ttttgcacca cttgttttaa gctttttctt
     6541 gaaccgaaga gcagggatca tggctggtat tttccaagca gtatctgcac ttttcatttt
     6601 ttatgattcg attggcacga actttctaac gattatcttg atgtcttatg ttttctctgg
     6661 gctcatggca acgattgtca gacgcaaacg gatctcagaa caaggattat ctgctgccat
     6721 gtgggtcatt atttttcctg tgatgatgga tgtcattttg atcgtctatc aaggaatgag
     6781 ctttggtgat gcaacgactt ggttaatgct tgtctgcggg ttcactggtg cactcttctc
     6841 attcttgctg acgatcggtc tacacccata tattgaattg atggtcaatg atgacagtgt
     6901 aattgtatta aatgaactca gcaatccaaa tcatccttta ttgaaacagt tgctggaaga
     6961 agcgccaggt acttatcatc atagtatgat ggtagctaat ctaagtgcta acgctgtagc
     7021 agaaatcggc ggacgttctt tgctaactcg agtagcttgc tattatcatg atatcggcaa
     7081 gatcaaacat gctagctttt tcgttgagaa cttgccttca ggagcggaaa acccgcacaa
     7141 tttcttattg ccagaagaca gtaaacatat tatctttgga catgtgacag atggagccaa
     7201 aatattggaa gattataata tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg
     7261 aaccacgcta atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga
     7321 agagcaattc cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat
     7381 tgctgacagt tgtgaggctg cagtacgggc aatggatcat ccgacgagcg aaaaaatcga
     7441 aagttttgtc cataatctta tcgaagagcg tatttccgat ggtcaattag acgatagtgg
     7501 actgaccttg aaagaaatcc gaaaagtgga aaaatcattg atcagcggtt taagtagcac
     7561 cttccattct cggatcaaat atcctaaaat gaaaacagaa gttgagaaga taaaggaaga
     7621 acaagaagaa tagaaagaag cgatgtataa tggatattac ctttatggat gaaacaaacc
     7681 aagtctcaga agagaaaatg aaagaaatcg atgacttgct gcaatttgca gcatcttatt
     7741 taaagctgcc agaggaaaca gaaatgtctg ttactttcat ggacaatgcg gcaatccaag
     7801 taatcaatcg tgattaccgt ggaaaagatg caccgacaga cgtcatcagt tttgctctgg
     7861 aagaagaagg cgaagacgaa atcccagtga tttttgaaga tggagagaat ccattgcctc
     7921 gtgtactagg agacatcatg atttctattg aacgtgctgc tgaacaagca gaagcttacg
     7981 gccattcata tgatcgggaa cttggctttt tagcggtaca tggattcttg catatcaacg
     8041 ggtatgacca tatgacacca gaagatgaga aagaaatgtt tggtttacag aaagagatct
     8101 tggatgccta tggactcaaa agataaaaac gtagagaaaa acaaacattt cctgatgtct
     8161 gtggaatttg ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa
     8221 catgttgcgt tcggtatcct tgctttattt atgggcgtga ttttccagct tgatcggata
     8281 gagtggttat ggctgctttt agctgttttt ctcgtttgga tagtagagat catcaatact
     8341 gtttttgaaa atgtagtgga catgtttacg gattttcatt ttcatccgat tgggaaaaaa
     8401 atcaaagata tggcagctgg agcagtttta gtgaccgctt gttttgctgt catcattggg
     8461 ttgattttat ttgtccctaa aatatatcat ttgttgttta attaaaaagg agaacgtata
     8521 tgccagaaca taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca
     8581 cattattgaa ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa
     8641 ctagaaataa aatccaagga gtatatacta caccagaagc tcagctgatt tttatcgata
     8701 caccaggaat ccataaacca aagcatcgtc taggagattt tatggtagaa gcagcttaca
     8761 gtgcgttgaa agaagtggat gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc
     8821 ggggagacga ttttattatt gaacgtctaa aaaacgtgca ttctccagtt tatctgatta
     8881 tcaataaaat cgataagatc catccagacg agttgctggg aatcatcgaa gattattcga
     8941 cgcagatgcc ttttgcacaa gtgattccga tctctgctac agaaggaaac aatgtcgaac
     9001 gcttaatgga tgtattagtc agtgaaatgc cagagggccc acaatatttt ccagatgatc
     9061 aagtgactga tcatcctgaa tactttatcg tttctgaatt gatccgtgag aaagtcttgt
     9121 ttttaacacg agacgaagtg cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa
     9181 acgagaatga taaaatccat atccaggcca cgatcatcgt cgaaagagat agccaaaaag
     9241 gaatcattat cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg
     9301 atatcgaaaa tcttttagga gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag
     9361 actggcgaga caaaaaagtc tatctgcaag actttggcta ccgaaaagac gaatactgat
     9421 attctgaact tcttataggt taagtataga aataaactgg ataaaaatcc gcacgttgag
     9481 aaaaacggcg gaagatcgaa acataaataa tctggatcgg cagaatctgc ttttccagat
     9541 ttatttttta ggagggagaa tatgagccag cttgcagaat cgaaaggctt gattttatct
     9601 agccgtaatt acaaagaaaa agataaatta gtcaaaatct ttacggaatc ttctggcaaa
     9661 atgatgtttt atatcaaagg aatccatcgg caaaatcagc cattagctcc ggctttgctc
     9721 ccctttaccg aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc
     9781 agtgtcaaag acgtccatgc gtttcggaac atccaagaag atatcttttt gagcggttat
     9841 ggtacctatt tgctaaatct agtagatgca gcaatcgaag atcatcaata tgacccgcat
     9901 ttattccaat ttacgcaaca agctctgcag cggatggatc aaggagatga tgcagaaatc
     9961 atcacgaata tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc aatttggacg
    10021 cactgtgtcg tatgcgggga aacaaaagga aaatttgact attcttccaa atctggcggt
    10081 gtgatatgcg agaaacactg gccaatggac gaacaccgtt atcatgcaga tcctcgcgct
    10141 gtttatttcg ttcgaatgtt ttcggctatt tcttatgata agatcagtgg aatccacttg
    10201 aaagaagaaa cgaagcaggc aatccgtgac ctcattgacc agttgtatga tgaatatgtc
    10261 ggtattcatc taaaaagcaa aaagttcatt gatcaaatga agtcttgggc acacgtatta
    10321 aagacgccag agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa
    10381 gatacaacaa aaaaatcaaa actatttgac aaatacaggg acttccgtct attattaacc
    10441 gtagattaaa tagatcgttc actgagagga tctattatat aaacaagaaa actgcaaaat
    10501 gaatacgtac attgaccaag atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg
    10561 gaagccggac ccaaacgatt tttgaagagg cacttgcgag tacgataaat gaagctgccg
    10621 aatctttatt cggaagtagg gtggaaccgc gaaattattc gtccctatgt ctgtaatcag
    10681 acatagggac tttttgtcgt tttctttcct gtattcttat tgacagcaac gcttaaaaat
    10741 tgaaaggggc aaaacagatg agtaaaaaat tgaccgtcca agatatgatt ttaacattac
    10801 aaaaattttg gtcatcaaac ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag
    10861 caggtacgat gagcccatat acattcttac gggcaatcgg accggaacca tggaatgctg
    10921 cttatgtaga gccatcccgc cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt
    10981 tgtatcagca tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat atccaagagc
    11041 tttatttaga aagcttgcgt cttttaggaa ttgatccatt agaacacgat attcgttttg
    11101 ttgaagacaa ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc
    11161 ttgatgggat ggagatcaca caattcacgt acttccagca agtcggagga ctaccttgta
    11221 aaccagttac ttctgagatc acttatggac tagaacgtct tgcttcatat attcaagaag
    11281 ttgaaagtgt ctatgatctg gaatgggctg atggtgtgaa atatggcgag atcttcaaac
    11341 aaccagaata tgaacattcc aaatattctt ttgaagtaag tgaccaagac ttgctactaa
    11401 gcaactttga ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat cttgttcatc
    11461 cagcgtatga ttatattttg aaatgcagcc atacattcaa tttattagat gctcgcggtg
    11521 cagtatccgt aacagaacga gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg
    11581 tagccaaaat ctttgtggca gaacgcgaaa aattaggatt ccctttattg aataaaaaca
    11641 cagcaaatcc agaaaacatg aaggaggctg aataagatgg caaaagactt attacttgaa
    11701 atcgggctgg aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa
    11761 aaaacagcaa aatttttaga tgaaaaccaa ttgacttatg atacgatcga aacgttttct
    11821 acaccgcgcc gcttagcaat caaagtaaca aacattccag aacgtcaagc agacagtgaa
    11881 gaagaagtca aaggaccagc caaaaaaatt gctttggacg cagatggaaa ctggacgaaa
    11941 gcagctgaag gatttgtccg cggacaaggt ctgacgacag atgctatcac ttttagagaa
    12001 ttaaaaggcg tggaatatgt ctatgtaacg aaatacactc acggcaaaga agcgaaagaa
    12061 gtattgacag gtttagcaga tgtcattaaa agcctcactt tcccagtaac gatgcattgg
    12121 gcagaatacg attttgaata tatccgccct attcactgga tcgtggcatt gttagatgac
    12181 gaagtaatcc cgtttgagat cttagatgtc aaaacaggca gagcctctcg aggacatcgt
    12241 ttcttaggcg aagatgtgac atttgcgcat gcaaaagaat atgaagaaaa attgaatgaa
    12301 caattcgtta ttgcttcacc gcaaaaaaga caagaaatga tcgttacaca agcaaatgaa
    12361 ttagccgaaa aaaatcactg ggttattgat ctagacgaag atttactaga agaagtcaac
    12421 aatttagtcg aatatccaac tgtcttttct ggcgacttcg aagaaaaata tctttctgtt
    12481 cctgaagaag tactagtcac ttctatgaaa gaacatcaac gttatttcga ggtacggaac
    12541 gatcaaggta tgctgcttcc tcattttatt tctgctcgta atggtgatcg ggtaaaattg
    12601 gaaaatgtgg ttcgaggcaa tcaaaaagta ttgacggctc gtttagaaga tgcggaattc
    12661 ttttataatg aagacaagaa agcttcaatc gaccaatacg ttgaaaaact aaaaactgtt
    12721 actttccatg aaaaaatcgg tagtatctat gagaaaatgc agcgggtaca agtcattgcc
    12781 cgtatcattg gcgaacaagt aggactatct gaggaagaat tgatggatct gcaacgagct
    12841 gctgaaatct ataaatttga tctagtaacc aacatggtag gggaatttcc tgaactacaa
    12901 gggatcatgg gcgaaaaata tgctctgctg aaaggcgaga aacctgctgt agcaacagcg
    12961 attcgtgagc attacatgcc tatatccagt gaaggagagc tacctcagtc tacagtaggt
    13021 tctgttttag cgattgcaga taaattagat agcatttttt ctttctttgc agttgggatg
    13081 attccaagcg ggtcaaacga tccttatgcc ttacgtcgcc aagcatacgg tgtcgttcgg
    13141 atcatcgaat cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac gattagtgat
    13201 gttatcagta aagatacgga tcgtttcggc attggtttat cagaagggca acaacaggtg
    13261 atcgacttta tcaaaggccg tctgcgccaa ttattgacaa cgaagaatat tcgtcatgat
    13321 gtcatcgaag ctgttttgaa tgcagaacaa aaagatctga caaaagtatt tgcagcagca
    13381 caattgttca agcagcatct tgcagatgaa gacttcaaac catcgatgga agctttgaca
    13441 cgtgtcgtga acttagcgaa aaaagcggag cttgaaaaac aatcagaagt agaccctgaa
    13501 ctgttcgaaa atgaagcgga gaaagaactg cacaaagcag tagaggcaat gaaaaaaagc
    13561 tttgctgacc aaacgatcaa cgaaaattat gatcagcttg taagccttcg cccactgatc
    13621 gaaaattatt ttgatcagac gatggtcatg gcagaagaac cagcagtacg taagaaccga
    13681 ttaacgcaat taagtcaaat tgctgccatg gctttgtcgc tagcaagttt agatcaaatc
    13741 atcacaaaat aatttctaat atgtaaataa accagagaat aggcacaaaa
//
LOCUS       DAYYOA010000070        13657 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000070 DAYYOA010000000
VERSION     DAYYOA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.070"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..475
                     /locus_tag="ACHBKA_001839"
     CDS             137..475
                     /locus_tag="ACHBKA_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445518.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="ACHBKA_001840"
     CDS             complement(680..1255)
                     /locus_tag="ACHBKA_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFM9445519.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1987..2568)
                     /locus_tag="ACHBKA_001841"
     CDS             complement(1987..2568)
                     /locus_tag="ACHBKA_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9445520.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2751..3377)
                     /locus_tag="ACHBKA_001842"
     CDS             complement(2751..3377)
                     /locus_tag="ACHBKA_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9445521.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3399..4730)
                     /locus_tag="ACHBKA_001843"
     CDS             complement(3399..4730)
                     /locus_tag="ACHBKA_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM9445522.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4885..5286)
                     /locus_tag="ACHBKA_001844"
     CDS             complement(4885..5286)
                     /locus_tag="ACHBKA_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9445523.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5372..5578)
                     /locus_tag="ACHBKA_001845"
     CDS             complement(5372..5578)
                     /locus_tag="ACHBKA_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445524.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5673..7175)
                     /locus_tag="ACHBKA_001846"
     CDS             complement(5673..7175)
                     /locus_tag="ACHBKA_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM9445525.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ACHBKA_001847"
     CDS             complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ACHBKA_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9445526.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7746..8363)
                     /locus_tag="ACHBKA_001848"
     CDS             complement(7746..8363)
                     /locus_tag="ACHBKA_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9445527.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8584..9957
                     /locus_tag="ACHBKA_001849"
     CDS             8584..9957
                     /locus_tag="ACHBKA_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9445528.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10032..11144
                     /locus_tag="ACHBKA_001850"
     CDS             10032..11144
                     /locus_tag="ACHBKA_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9445529.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11216..12853)
                     /locus_tag="ACHBKA_001851"
     CDS             complement(11216..12853)
                     /locus_tag="ACHBKA_001851"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM9445530.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13021..13629)
                     /locus_tag="ACHBKA_001852"
     CDS             complement(13021..13629)
                     /locus_tag="ACHBKA_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9445531.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3681 a         2878 c         2450 g         4648 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     1681 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     1741 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     1801 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     1861 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     1921 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     1981 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2041 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2101 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     2161 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     2221 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     2281 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     2341 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     2401 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     2461 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     2521 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     2581 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     2641 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     2701 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     2761 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     2821 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     2881 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     2941 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3001 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3061 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3121 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     3181 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     3241 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     3301 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     3361 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     3421 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     3481 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     3541 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     3601 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     3661 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     3721 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     3781 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     3841 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     3901 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     3961 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4021 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4081 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4141 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     4201 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     4261 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     4321 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     4381 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     4441 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     4501 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     4561 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     4621 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     4681 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     4741 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     4801 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     4861 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     4921 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     4981 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5041 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5101 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     5161 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     5221 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     5281 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     5341 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     5401 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     5461 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     5521 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     5581 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     5641 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     5701 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     5761 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     5821 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     5881 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     5941 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6001 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6061 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6121 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     6181 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     6241 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     6301 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     6361 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     6421 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     6481 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     6541 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     6601 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     6661 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     6721 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     6781 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     6841 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     6901 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     6961 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7021 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7081 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7141 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     7201 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     7261 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     7321 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     7381 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     7441 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     7501 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     7561 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     7621 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     7681 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     7741 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     7801 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     7861 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     7921 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     7981 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8041 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8101 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     8161 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     8221 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     8281 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     8341 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     8401 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     8461 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     8521 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     8581 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     8641 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     8701 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     8761 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     8821 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     8881 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     8941 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9001 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9061 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9121 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
     9181 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
     9241 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
     9301 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
     9361 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
     9421 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
     9481 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
     9541 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
     9601 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
     9661 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
     9721 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
     9781 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
     9841 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
     9901 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
     9961 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10021 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10081 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10141 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    10201 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    10261 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    10321 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    10381 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    10441 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    10501 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    10561 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    10621 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    10681 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    10741 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    10801 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    10861 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    10921 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    10981 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11041 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11101 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    11161 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    11221 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    11281 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    11341 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    11401 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    11461 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    11521 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    11581 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    11641 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    11701 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    11761 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    11821 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    11881 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    11941 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12001 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12061 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12121 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    12181 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    12241 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    12301 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    12361 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    12421 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    12481 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    12541 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    12601 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    12661 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    12721 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    12781 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    12841 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    12901 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    12961 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13021 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13081 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13141 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    13201 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    13261 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    13321 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    13381 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    13441 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    13501 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    13561 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    13621 tgaattcata ttaaactcct ttttttctcc gtttttt
//
LOCUS       DAYYOA010000071        13449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000071 DAYYOA010000000
VERSION     DAYYOA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.071"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..267
                     /locus_tag="ACHBKA_001853"
     CDS             100..267
                     /locus_tag="ACHBKA_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM9445532.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(329..2140)
                     /locus_tag="ACHBKA_001854"
     CDS             complement(329..2140)
                     /locus_tag="ACHBKA_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM9445533.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2137..2502)
                     /locus_tag="ACHBKA_001855"
     CDS             complement(2137..2502)
                     /locus_tag="ACHBKA_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM9445534.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2665..3060)
                     /locus_tag="ACHBKA_001856"
     CDS             complement(2665..3060)
                     /locus_tag="ACHBKA_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9445535.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3078..4040)
                     /locus_tag="ACHBKA_001857"
     CDS             complement(3078..4040)
                     /locus_tag="ACHBKA_001857"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM9445536.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4040..4252)
                     /locus_tag="ACHBKA_001858"
     CDS             complement(4040..4252)
                     /locus_tag="ACHBKA_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM9445537.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4273..4971)
                     /locus_tag="ACHBKA_001859"
     CDS             complement(4273..4971)
                     /locus_tag="ACHBKA_001859"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9445538.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4991..5533)
                     /locus_tag="ACHBKA_001860"
     CDS             complement(4991..5533)
                     /locus_tag="ACHBKA_001860"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM9445539.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5665..6669)
                     /locus_tag="ACHBKA_001861"
     CDS             complement(5665..6669)
                     /locus_tag="ACHBKA_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9445540.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6666..7655)
                     /locus_tag="ACHBKA_001862"
     CDS             complement(6666..7655)
                     /locus_tag="ACHBKA_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9445541.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7652..8458)
                     /locus_tag="ACHBKA_001863"
     CDS             complement(7652..8458)
                     /locus_tag="ACHBKA_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9445542.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8624..9580
                     /locus_tag="ACHBKA_001864"
     CDS             8624..9580
                     /locus_tag="ACHBKA_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9445543.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9657..10175)
                     /locus_tag="ACHBKA_001865"
     CDS             complement(9657..10175)
                     /locus_tag="ACHBKA_001865"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9445544.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10263..10412)
                     /gene="rpmG"
                     /locus_tag="ACHBKA_001866"
     CDS             complement(10263..10412)
                     /gene="rpmG"
                     /locus_tag="ACHBKA_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9445545.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10640..11086
                     /locus_tag="ACHBKA_001867"
     CDS             10640..11086
                     /locus_tag="ACHBKA_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9445546.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11281..13176)
                     /gene="asnB"
                     /locus_tag="ACHBKA_001868"
     CDS             complement(11281..13176)
                     /gene="asnB"
                     /locus_tag="ACHBKA_001868"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM9445547.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4193 a         2844 c         2292 g         4120 t
ORIGIN      
        1 aagggttttt tgcaaaggtt tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt
       61 ccataataga tataacaaat aataaatgag gtggattcaa tgaaaaaatt tgtatctgga
      121 atattagtag gaagtctggc aacagccgct gccgttgctg gtttggttgc ttctgtgaaa
      181 aaaacagtga tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa
      241 gcaatgcgta aacgcgtttc tcgttaataa ttatttaaaa atacgcatga agactttcta
      301 aaaaacagat aagtcatcat gcgtattttt atttgtcagt caatataagt tgtgctattt
      361 tatctgcctg tctcagattc catggatctg tataaggtct agctactgct gcagttagcc
      421 aatccacacc ttcaacaggt attccatagc aataaaagtc agactggatt tcccctgaag
      481 catcaatcac gcgattcgta cctcgctcaa catctatagc ttctgaatga taacttcctg
      541 tattatctga aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt tttatgacag
      601 gattacttga acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc
      661 tgatttcttt atcttgtaga gaaataacat agcggttgtg cttttgctga atatcaaatg
      721 gcggttcgat caaatgaaaa atacccgcat ctatcagtgc tgctaattct ttcgtccgaa
      781 tgattggcgg accgatcgtt aaaaagctat ttagatgagt aaaccatccc catagtattt
      841 ctttgtattc cttagcagaa aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc
      901 agtcttttaa tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct ttttccgctt
      961 cttttatctg cccttcaata aatattcggc tagcttctga aaagggctca ttctgatagg
     1021 caagaggagt ttctaaatac gaccaatccc atctataagg gattagtgct ggatgctttc
     1081 cgagacactc ctctgaagag agggagagaa aatcatgctc aaaatttttc tttggaatcg
     1141 gcaagtgata ttcttcgatg attttcttgt aataaaacaa ctcaacgtct tttttcagat
     1201 agtcaaaaaa tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc aggttttctt
     1261 ttgttagtaa ccgtggccat gccattgctc cgccttgctt ttgatttctt cctcgagggt
     1321 aataagggag cccccttctt gatccactgt atacaatcgg ttccttgcct gatggatgat
     1381 agaccagttt tcccttttcg ttctcgaagc gccctcctct tgatactgta aacaatccga
     1441 cataatcaaa aaaacttaag cctagacctc ttaaaaagac attttcttta ggcggtatct
     1501 tcgaaagatc tacatctgca ggattagaag gaagctgata cagtaaaaca tttttctttg
     1561 cgtagtctac ccacactctt tcttcttctg tctcatcatt ttcccagtga ccgcttgcta
     1621 aaacaagctg atcgatagaa tagctttgct gatctgtctg taaaagataa ccatcttttc
     1681 ttttgcttac tttttttact aaagaataaa caaactgaat agaagctgga aattcttctt
     1741 gcaactttga gaaaaaccag cgttgataga gtccgtataa acatcgagta gattgctgat
     1801 ctttttccag acgattgatt tcttctataa ttagttgctt atttttggtt accgtctgtt
     1861 cgatataatc tgtagcaggt cctttactcc attgaaacaa gttgggtccc tgtgttacca
     1921 atcctccact ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa acagaattca
     1981 ttaataactc cggtggctga ttcaaccgcc aaactcgacc tccaggacca ttgggatcga
     2041 aaagaaaaag ttcaagagaa tgtctgtcct gattattatg gagaatcctt tctgctacac
     2101 ttaatcctct aggtccggca ccaatgatcc ctattttcat ttgcatgcct ccatttcgat
     2161 aaattttttc aataaaacag catggttctg atgttcattt tttgcaccat aaacaaaagt
     2221 aatgactgga tgttctttca taagttgact gagtttaaaa atagcttgct tcttttcttc
     2281 ttcgaaaatc aattcttgct ggtatttctt tgcaaatgaa gcaaatcgtt ctggtacatg
     2341 gtcaaaagtt ttgcgtgctt cattactagg tgcaatttct ttcaaccaca gatctaactg
     2401 cgctttttct tttgaaattc ctcgtggcca gaatctatcc acgagtattc tatagccatc
     2461 ctctttttca gcatcaaggt aaacacgttt taatttgatc atagttgttt ccttctttct
     2521 accttttcgc ttagcttata tatgtagata atagcacaaa ataaaaaaaa tgaacggcac
     2581 aactatccat tgcgccgttc atgtgatctt ggtcaaaatc taatgattga ttcggttttt
     2641 aaagctgtca tacaatcatc tgttttatac ttttttactt ttgatttttc cagaccatcc
     2701 gtcatatcca cccttaagta aataaatctc ttgataacct gctttacgta atttatttgc
     2761 tgtacggatg ctcaaacttt tcttttgatc gtaaatatag acaggctggt cttttcgaat
     2821 agatcctaat gaatctttca aaacagtgta agggatgttt cttgcaccta aaatatgccc
     2881 agcatcaaac acatcttttt cgcgaacatc gattacttgt gcttttctca ttccttctcg
     2941 aaattcttct tctgtcaaag tcgttgctga acgtttggcc attactcgta gatagacttc
     3001 ccaaaggatg attgcaagca caatcaatag caaaatgata ttaattaccc ataaaaccat
     3061 gtaatgcctc cgttttttta agcaaatttc aaggctaatg aggcagcacc aatgacacca
     3121 gcttcattac caagctctgc taatttgatt tttgttgatt ctcttacttg cgggaaagta
     3181 aattctttaa aatatttctc tacacggcta cgtaaaaatt ctcctgctgc cgatactcct
     3241 ccacctaaca cgatacttga cggatttaat gtattcccca agtttccgca agctaaacct
     3301 aagtagaagc atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt
     3361 tcaaagatat ctttacttgt tacttcttct ccgttgtcta gcattgcttt cagttttgaa
     3421 tcccctgcgt attcttctgc caagaaacga cctaggcgta ccacacctgt agcgctagat
     3481 acagtttcta aacatccgcg tttaccgcaa gtacattcga acccttcagg atccacagtg
     3541 atatgtccga tttctcctcc agctccgctc acgccgtgaa tcaaatttcc gccagcaacg
     3601 atacctccgc caacacctgt tcctagagta acgaatacaa cgtctggatc gttttctcca
     3661 gcacctttcc atcgttcacc taaagcagca acattcgcgt cattatcgat agcgaatttg
     3721 attcctgttc ctgcctcgat cagttttttc acaaattgaa cttctgtcca gtttaagtta
     3781 tacgcaccaa taaccgttcc agcttcgctg tccacgcttc ctggggtacc catgccgatt
     3841 ccgataaagt cttcggcttt catattatat aaattcaaac gatgattgat tgattcaatg
     3901 atttcaggaa cgatatgctt accatcatcc aaaatattgg tatcgatgct ccatttttgc
     3961 tgaatctctc cttctggtgt taaaattgca aattttgctg ttgttccacc taaatcaatc
     4021 ccaataatct tcttgtccat tacaaatttc ctttctttcg gctttcttca attcggtgct
     4081 ctctttttaa tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa cggctatcat
     4141 acagattacg caattcgata gtcattaatt caatgtcata gatgcgtgca cccacataga
     4201 taaagatgcc aaattttttg aacagctgct gtacatcata taagctctcc atctttaagc
     4261 acttccttca tattatacaa gtaaaccata tttttttaaa cccagtaaaa tacatattac
     4321 aaaaagaaat acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg
     4381 ttgtttcggt agacccaaaa cattccctaa cactaatccg ccaattagcc caccgacatg
     4441 tccccaaaca tctacagagg tatccagaag accaaaaaca aatgtcaaca caataaataa
     4501 aagaaaatgt ctgactagct gctggatcgc tgggttatgt ttaaaatgaa accctaagat
     4561 aaacaacgca ccgaacaaac cgaatatcga agtacttgca ccaccagaca gaacattcgc
     4621 ttcgttaaaa gcaaagcttg ctgtatttcc cattatcccg ctgaacatat aaatcaagaa
     4681 aaaacgccaa tggccataga ttgcctcgat ttgctgcccc ataaaataca gtatcagtga
     4741 gttcattacg aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta aacgccacca
     4801 ttcattgaag tgaacaagat aaggaccata cattccggca taataaggga tctcaagtcg
     4861 cggaaataat tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca
     4921 taacggtcgt cttaaccatg cacgcaattt tatttgctgt tgataattca tgaaaacctc
     4981 ccatttcatt ttagtcaata aacaattgtt ggatcttctg atcaaagctc tccggttgcc
     5041 attgttcatt caattgttca cgaaaaacaa ggctacatgt ttttttatga aaattattta
     5101 aataccggtc gtaataacca ccgccaaaac cgattcgata accggctcta ttgtagataa
     5161 tacctggtac aatgaggaga tcgatcatct ccggcaaata gagcttctct gattgtggct
     5221 caagaacacc aaaactcgaa cgtacaagaa cggactgttc atccatttca taaaacgcca
     5281 tctgacgttt cggcaaagtt ttaggtacaa ctattttctt ttgttctttt tctgctcgtt
     5341 ggataaccgg ctgtgtatcc agttcaaatt cattcgataa agtcgtccct atcacttttg
     5401 ctgacttcca gtactcgctg gcgaatagct gtttatagat tcgctgttct ttttcctttt
     5461 tcttgccact tttggccaga tttttcagct tttcgattcc tttccggcgt aatcttactt
     5521 tcaattggtc caaaaactca cccttccttc tctcataaaa tggtatagga aaaaaagaaa
     5581 aatagcaatc gaactttcaa ataaatgcat aaatagaata aaaagaagac ctagaaatga
     5641 ttggccttcc ttttttaatg aatttcaact gttcagcgcg tgtttttgtc tgataaaaag
     5701 tgcatacaga aaatatggcg caccaaaaag ggcaatcaaa attcctgtag gtacttcccc
     5761 acttggtaga atcactctag caaccatatc tgcactaagc agaaaaatgg aaccgaacaa
     5821 accgctcaga acaaaggtcc agttattttc tttacgtacg actagcttcg cagcatgagg
     5881 agctagtagt cctacaaaag ataaactacc tgtcaccgct acactaccgc ttgccaaagc
     5941 aacagctaac aacaaaagga ttttttgact tttagccacg ttaaggccta atccttgcgc
     6001 gcgttcttga ccaaatgata gcaatacaat cctctgtgca taaagtcctc ctagcggaat
     6061 taagataacc gcccatggaa gcaatgccca aacataaggc cagctcgcac cccagattga
     6121 tcccgctaac catgcattca caaatccata attttcctta gataattgga tcgtgcatag
     6181 caatgttaat gcagaaaggc cggcattaac ggcaataccg gacaataaga ttcgattcat
     6241 acccaaaaag ccatttcgat cataggccgc aagatagaca cagcaagcag ctaaaacacc
     6301 acctagaaag gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa
     6361 ataaagcatc actaaaaatc ctgctccggc attaatgccc aatatactag aatctgccaa
     6421 ttcatttctt gttactcctt gcaagatata accagaaacg ccaagacaag ctcctgctaa
     6481 taaactgatc agtaatcgag gaactcgaaa ttctctaaca atcagcgcca aagaagatga
     6541 ttctttccct atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg taccaatcat
     6601 taaactacct atagacagga tgacaagtac aaaaagcaag aaatagaacg tccaatatat
     6661 ttttttcacc gttgttccct ccttacttgt atcagtaaaa acggaacgcc aatcaatgca
     6721 atgactaatc caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc
     6781 aacataacca atccaccgcc aactaaaaaa gagacaggta gaacataacg atagtccgtt
     6841 ccaaccattc cgcgaacaac tggcggaacc aacagcccaa taaatgaaac aggtccgact
     6901 aacgaaacgg caatggctgc taacactgca actagacaga aaacaatcaa ccgcgttcgt
     6961 ttaggatgac ttccaagagc taatgcatgt gtttcactca aatgaatcaa agatattgct
     7021 tttcctaatg aaaaagaaac caaaacagca agtatatata aaggaaacaa tactgctaat
     7081 tgtttccatg tgatattggc agctccgcct acaaaccaaa aagcaagatc ctgctgcaga
     7141 ttaaagaatt gggtaagtgc ttggctgata gcagtaaaaa aagaactaat agctacgcca
     7201 gctaaaatca gctgtaaagg attcatgccc attttcgaaa actgcaaaaa accaaatacg
     7261 agcaaagtta cagcaaaaga acctgccaat gacattagaa acacagaaaa atcagaagct
     7321 ttaggccaaa aagcaaaact aagggccatt gccaacgaag cacccgcgtt gattcctagt
     7381 aagcctgaat ctgctaaagg atttcttgtc accccttgca tcaatgcacc gctcaatgca
     7441 aaacaactcc ctacaagaaa tgctcccgcc acacgaggca aacgaatatt tcgtatgatt
     7501 tggtgacttt gttttgatct atcaaaatca aacaacgcct gccaaaaatc tgctttttcc
     7561 acagtcgctg cgccatttga aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc
     7621 aaaagcagga gtctattttt cttcatagtc ctcataattg atcctcctgc tcttttggca
     7681 acaaatcata ggttagaaaa actggtttat ttgttcttgg aagcgtgaca atcgttgcat
     7741 caatctcaaa tatttttttc atacactctt ttgtgatcac ttcttcaggt gtaccttctg
     7801 tgatcaactt accgtttttc attgctaata aataatctga aaaacgtgaa gcatgattaa
     7861 tgtcatgaat cgacatcaag atcgttgttt gtttcatttg attgatttca gtcaataact
     7921 gtaggatctc taattggtgt gccggatcta aaaatgttgt cggttcatcc aagatcaaga
     7981 tatctgtttc ctgagcaagc gccatagcga tccagacacg ctgggtttgt ccaccagaca
     8041 gcgttgcgat atcccgcata cgaaatccag ctagacctgt agcatccaac gcccactcaa
     8101 ttgcttcatg atcttgcgtg tccatgccac tgaacagttt ttgataaggg tatcgtccgt
     8161 aagatactac ttcttctact gtcatacctg tagggtgttc actagtttgt gctaataatg
     8221 caattttttt tgcaagttct tttgaactgt atgtagagat atcagagtta ttgacaatga
     8281 tctttcctga tttcacaggt agtatacggg taaaactttt caataaagta gattttccac
     8341 ttccatttgg cccaatcaaa ctagtgattt tcccttcagg aatcgtaaca gacatgtttt
     8401 caatgatttt tttgttttcg tagccggctg caatgtcagc tgctcttaat ctcgtcattt
     8461 cacccatcct ttcgctgaaa atcattctca attatgacgc tgtttcaaca tttcgtcaag
     8521 cgctactttc atatctatag ccaaatgaag cggttgacag cgttcgaaat atcaggaata
     8581 ataaccaatg agaaagattc tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa
     8641 agtattcaca ttatctagca tattgctgct ggctggagta ctgagcgctt gctccccctc
     8701 ttcagataaa gaaacagcta caactgccaa ttcgacgact tcatccaata gtactcatac
     8761 agtcacagat acacttggac ataaagtaga gattccaaac gcgcccaaac ggatcatcgg
     8821 cagttactta gaagactatc tgattgcact aggggaaaaa cctatcgccc agtggactgt
     8881 tggcagtggt tccattcaag attatctgca agatgattta aaggatgtcc caacgatttc
     8941 ttatgacctt ccttatgaga aagtgttaag ctttgagcct gatttgctgt tgatttcttc
     9001 ttccgctact gtcgaaggcg ggaaatacga acaatatagc aaaatcgcgc caacttatgt
     9061 tgtaaaaaac ggaacagatg tcacatggga agaacaacta aaagatgtcg gcaaagcttt
     9121 ggcaaaagaa aaagaggcag aaaaaatcat ttccgattat caaaaatcca tcaagaaaac
     9181 tcgagaagaa ctagcagata aaatcgagaa caagacagct gcagtgttgt gggtgaccaa
     9241 caactcagcc tttatggtca gcaaaaatcg gtcaagtgga agaatcgtat acgacgatct
     9301 ggaatttggt gttcctgatc tagtggaaga ggtctccaaa aaagccactt ctgactggtc
     9361 tgctgtttcc tctgaaaagc tagctgaatt agatgctgac tacatcatcc ttgtgaacag
     9421 tgacaaagat gctgccatgt tcaatgaacc aagctggaag aatctaaaag cagtgacaga
     9481 caatcatgta ctgcaattcg gacctgaatc tagttggcta tataacggac ctattgccag
     9541 tcaggaaatg gtcaatgata tcaaagaaaa actaaattga ttttaactaa aaaaagcatc
     9601 ttcacgatta gtcgtgagat gctttttttc ttattaacaa actagacgca tagattttat
     9661 tcttcttttg aagcaaccct attttttact ctttcccatt tttgaaaacg atgggcatat
     9721 agattttctt gatccgttat cccctcatac tcctctataa gggcgaagtc tgaaaaatca
     9781 atattcccaa tatacgtatc gccttcaaac gtgctatcga tcaatgttcg ccagatccga
     9841 ccagtgtaag gaagaagttc ttgaaaaatc cgactaccac ccgaaacata gatatcttct
     9901 gagatcgttt gactatacga caaaagttca tcgatcgaat gcattacttc cgcattttct
     9961 ttcgtgcgaa aatttggttg cgttgtcaac acgatgatat ggcgataagg aagtgaaaga
    10021 tcccccattc cttcataggt ctttcttccc atcactaaaa ttcgatccat tgtatgttcg
    10081 cgaaagaaac gcatatcatt tggcaatcgc catggtaaca aaccattttt gccgatcaga
    10141 ccatttttat cttgtgccca catggaaata aacatattat ccgctcctta tgattttaga
    10201 tgttttgaga taaaaaaagt ctcaattctt tgaccagcaa ggaattgaga actttaaaaa
    10261 cattattttg tttcacggtg caaagtaact ttgcgttcgc gtgggcaata tttttgtttt
    10321 tccaaacgat caggattgtt acgtttgttt ttgcttgtta gatagttacg ttctttacaa
    10381 gaagtacact ctaaagtgat gtttacgcgc atgtttttcc ctcccatttt tacaagaatc
    10441 ctctaagatg actctcagac ttgattatct taccatgatt ttttctagat tgctagtgtt
    10501 tttcacgatt ttgttaagta aaactacttg acgataaata caacggtttt acttctcaaa
    10561 caaaatcttt ttctttttgt ataaataccc ttacacttgt ttatatagat tatgtttttt
    10621 aataaagggg gatactaata tgccatggaa catgaaggac tttcctgcat caatgaaaaa
    10681 cttagataaa ttgacaagaa aaaaagcaat cgacatcgca aatgcattat tagatgaagg
    10741 gtaccctgat aaccgtgcga tcccaattgc cattgatcaa gcaaaaaaat ggtatgaaga
    10801 tgctagtgaa tcagaaaaac gaactttcga aaaagaaaaa aatccaagca agacggatga
    10861 acacgatacg aatcctcgtg ctggaaaact gcttgattca gatgtgatcg tagaatatga
    10921 ggaagaacaa tggatcgtga aatctaaagg ggcaaaaaag gcaagcaacc attttgatac
    10981 aaaaaaagaa gcaatcgaaa aaggaaagca agtagcacaa aacaaagaat caactttgtt
    11041 catttataaa aaagatggga caaaagaaaa agaaatcgat tattgaacat atgtaaaggg
    11101 gttgtgacga aaatcgtgtc acaaccccct tatgttcgaa acaatggtgt tcctctgcca
    11161 actccgaacc ataagtcata atatctataa aacgggaaga gctgtttttt gaaattgttt
    11221 cttatcactc agtgttaaac ggtaggagaa aacctctttt ttattgatat ttgattttca
    11281 tcaaacagtc acatagtcga ttgcttctgc ttgcggaatc gtttcatcta caaagtagac
    11341 tttataccat gtcaagaaac tgaagattgt ccagattttt ctttgctctt ctttacgtcc
    11401 ttcgtgatgg tcatctagca acttcatgat tttttcttga tcaaaaaatt cttttgcaaa
    11461 atctgcttcg aataatgctt tgacttgttc gtagccgtgt tcttctttca accaagcttt
    11521 gattggcaca gggaatccta atttcacacg attcgaccat tcttccggaa gatgtttgtt
    11581 cgcagcttgt ctgaaaatat cttttgtgtt gttttgattc aataaaaact tacttggtat
    11641 ttgttgagcc aatttcatga cttcgatatc caataaaggt actcggactt ccaaagaact
    11701 tgccatagaa agtttatctg cctttaacaa gatatctttt ggcatccatt gatgaagatc
    11761 gacatattgc atcttattga cttcatcttt gattccttct gttttctcgt aatgaacagt
    11821 catgatttca tccacagatg gtgctgtttg atattttggt gttaacactt ctgatgcttc
    11881 tgattcttcg aacactcgag catgaccgat aaaatattct tttgcaggag caagtgattc
    11941 atataaatgg atccgtccat ggaagttttt catttttccg attttacgtc ctaaattata
    12001 gcgtgttcct tttggtaatt ttttcaatcc ttgagcaatc acacggatga attttgaatt
    12061 cgtatggaat ccatacgttt ggtatcccgc aaacagttca tccgcccctt ctccagattg
    12121 gactacccgc acactttgtg aagcaagatt tgctaaaaag tacaaaggta cgcaagaagg
    12181 atttgaatca ggttcatcta agtgatactg gatcaaaggg aatgctttga acgacatgtc
    12241 tccgtcgatc actgctgctg tattatctaa gtccaatagt tcggttagtt ttctggcttc
    12301 aatcgcttca ttataggttt tatcatcaaa cccgattgag aaagaatgat ctggtttcaa
    12361 tacagaggtt acataactgg aatcaacccc gctggataag aaagaaccaa cttctacatc
    12421 actgaccgta tgtgcttcaa tagaagcttg aacggtttcg tcgatttttt cgatccattc
    12481 ttgtcgggaa tgcgtttctt ttgtttcaaa atctgcatcc caatattgct gaatgtctat
    12541 ttttccatct ttatacgtat aataatggcc ttcaggcaaa cgatagacat ctttgaagaa
    12601 tgtttcgccg tttaatggag aatattggaa agtcatataa ggtttcaacg cttctttgtt
    12661 caattctttg ttaaaatcag gatgaggcag aaaacttttg atttctgaac caaacataaa
    12721 tgttccgttc atttctgcat aataatatgg tttgatccca aagtggtctc ttgcaccgaa
    12781 taactcattc ttttcattat cccaaatagc aaaagcaaac attccgcgga tcttttgcaa
    12841 taattctgtt ccccattctt cgtaaccatg caaaaggact tctgtatctg catgtgtttg
    12901 aaaaacgtgg ccagctgcaa tcaattcttc tctcaaaggc tggtaattat agatttcgcc
    12961 attaaaaata atgatctttg tccgatcttc attgtaaata ggctgagtac cgccctctaa
    13021 gtcaataata cttaatcttc tgaatcctaa agcaacatgt ttatcgatat attctcctga
    13081 actattaggt ccacggtgaa cgattcgatc catcattgta ttaataattg tttttttgtt
    13141 gtccttgtcg ttaacaaaac ctacaatacc gcacatttaa ctttcacatc ctaatataaa
    13201 attactgttt atttcattca tatgcatctt atcatagaat aaatagcgga cgaatatttt
    13261 ctctcttaca atttcgtcac gtgaaaacaa aagatgaaag tttctgtttt tacagaaaat
    13321 aaagtcccaa tatctaattt accatgtttt cttttttgag gctaccttat tctcttcgtg
    13381 ttaaaaaaat attagtggat gctatttcca aaaaaaccag caaaaaaaga cacgacctaa
    13441 agatcgtgt
//
LOCUS       DAYYOA010000072        13429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000072 DAYYOA010000000
VERSION     DAYYOA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.072"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHBKA_001869"
     CDS             complement(<1..914)
                     /locus_tag="ACHBKA_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM9445548.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHBKA_001870"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHBKA_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM9445549.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACHBKA_001871"
     CDS             2006..2176
                     /locus_tag="ACHBKA_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445550.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACHBKA_001872"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACHBKA_001872"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM9445551.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACHBKA_001873"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACHBKA_001873"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM9445552.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACHBKA_001874"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACHBKA_001874"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM9445553.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACHBKA_001875"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACHBKA_001875"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM9445554.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACHBKA_001876"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACHBKA_001876"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM9445555.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACHBKA_001877"
     CDS             6751..7752
                     /locus_tag="ACHBKA_001877"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM9445556.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACHBKA_001878"
     CDS             7749..9110
                     /locus_tag="ACHBKA_001878"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM9445557.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACHBKA_001879"
     CDS             9070..10221
                     /locus_tag="ACHBKA_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9445558.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACHBKA_001880"
     CDS             10196..11365
                     /locus_tag="ACHBKA_001880"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9445559.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACHBKA_001881"
     CDS             11540..12919
                     /locus_tag="ACHBKA_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9445560.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACHBKA_001882"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACHBKA_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAYYOA010000073        13399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000073 DAYYOA010000000
VERSION     DAYYOA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.073"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..713)
                     /locus_tag="ACHBKA_001883"
                     /pseudo
     CDS             complement(15..713)
                     /locus_tag="ACHBKA_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            792..968
                     /locus_tag="ACHBKA_001884"
                     /pseudo
     CDS             792..968
                     /locus_tag="ACHBKA_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="ACHBKA_001885"
     CDS             complement(1144..1713)
                     /locus_tag="ACHBKA_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9445561.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="ACHBKA_001886"
     CDS             complement(1787..2110)
                     /locus_tag="ACHBKA_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445562.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="ACHBKA_001887"
     CDS             complement(2264..3076)
                     /locus_tag="ACHBKA_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445563.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3403..4278
                     /locus_tag="ACHBKA_001888"
     CDS             3403..4278
                     /locus_tag="ACHBKA_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFM9445564.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4292..4615
                     /locus_tag="ACHBKA_001889"
     CDS             4292..4615
                     /locus_tag="ACHBKA_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445565.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4612..5364
                     /locus_tag="ACHBKA_001890"
     CDS             4612..5364
                     /locus_tag="ACHBKA_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM9445566.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="ACHBKA_001891"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="ACHBKA_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACHBKA_001892"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACHBKA_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM9445567.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="ACHBKA_001893"
     CDS             complement(9828..12014)
                     /locus_tag="ACHBKA_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9445568.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACHBKA_001894"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACHBKA_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM9445569.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="ACHBKA_001895"
     CDS             complement(12236..12676)
                     /locus_tag="ACHBKA_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM9445570.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="ACHBKA_001896"
     CDS             complement(12750..13289)
                     /locus_tag="ACHBKA_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9445571.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DAYYOA010000074        13259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000074 DAYYOA010000000
VERSION     DAYYOA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.074"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1887
                     /gene="yfcC"
                     /locus_tag="ACHBKA_001897"
     CDS             148..1887
                     /gene="yfcC"
                     /locus_tag="ACHBKA_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM9445572.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2044..3228
                     /gene="iadA"
                     /locus_tag="ACHBKA_001898"
     CDS             2044..3228
                     /gene="iadA"
                     /locus_tag="ACHBKA_001898"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM9445573.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3339..3542
                     /locus_tag="ACHBKA_001899"
     CDS             3339..3542
                     /locus_tag="ACHBKA_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9445574.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3643..4305)
                     /locus_tag="ACHBKA_001900"
     CDS             complement(3643..4305)
                     /locus_tag="ACHBKA_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9445575.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4318..5820)
                     /locus_tag="ACHBKA_001901"
     CDS             complement(4318..5820)
                     /locus_tag="ACHBKA_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9445576.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6368..8047
                     /locus_tag="ACHBKA_001902"
     CDS             6368..8047
                     /locus_tag="ACHBKA_001902"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9445577.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8174..8653
                     /locus_tag="ACHBKA_001903"
     CDS             8174..8653
                     /locus_tag="ACHBKA_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM9445578.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8872..9552
                     /locus_tag="ACHBKA_001904"
     CDS             8872..9552
                     /locus_tag="ACHBKA_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM9445579.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9560..10891
                     /locus_tag="ACHBKA_001905"
     CDS             9560..10891
                     /locus_tag="ACHBKA_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM9445580.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10906..11868
                     /locus_tag="ACHBKA_001906"
     CDS             10906..11868
                     /locus_tag="ACHBKA_001906"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM9445581.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11979..12317
                     /locus_tag="ACHBKA_001907"
     CDS             11979..12317
                     /locus_tag="ACHBKA_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM9445582.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12396..13103
                     /locus_tag="ACHBKA_001908"
     CDS             12396..13103
                     /locus_tag="ACHBKA_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM9445583.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4221 a         2338 c         2907 g         3793 t
ORIGIN      
        1 tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa attattcatg
       61 tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt catcaaaatt
      121 actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg aaaaccgaaa
      181 cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg tgtagtcatc
      241 tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca agatattgaa
      301 tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga ttccttccgt
      361 tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact gcaagatcat
      421 ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt cattgctgac
      481 ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga tgatgtgctt
      541 tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa aacagccaaa
      601 atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt tgaaggacta
      661 gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct tgttgttgga
      721 ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta tgcgtttatc
      781 agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc tttttcattc
      841 ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt gatggttcca
      901 tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta tgtcgcatct
      961 caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc tcaagggatt
     1021 gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt agttgttact
     1081 gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa accagaaagt
     1141 tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac atctgatgaa
     1201 aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt tgtccttgtc
     1261 tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc ttctcaattc
     1321 tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga tggaatgaaa
     1381 gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg tacagcaatc
     1441 gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc atctgttcct
     1501 tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt tccagctgtc
     1561 attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt tacatcaaat
     1621 tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt agtaggtgta
     1681 tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga cgcttttaca
     1741 cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg ggcaaaatgg
     1801 gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat tttcatcatg
     1861 attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa agtaatccaa
     1921 acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga gtaaagatcc
     1981 tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa agaggaaaaa
     2041 tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg aaaaatggat
     2101 gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg cggctacgaa
     2161 ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg tttcattgac
     2221 tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg aacgccagaa
     2281 gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg tttgggaaca
     2341 gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct ggaagcagaa
     2401 ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac agtaacagat
     2461 tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga aatcgcagta
     2521 tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt agcagatgct
     2581 cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt aggcggagga
     2641 aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc gatcacacaa
     2701 tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt ggcatttgcc
     2761 aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg ggagaagaca
     2821 gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga tgtttcttta
     2881 gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga tgaaaacaat
     2941 catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat caaagaagct
     3001 gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag taatccagca
     3061 cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc agatctgtgt
     3121 atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga aataatggtc
     3181 caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc aacagctcta
     3241 actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga tgttaccctg
     3301 aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta aaaggacgtt
     3361 ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc aaagcaacag
     3421 acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac atcaaagaaa
     3481 aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac gaaaaaaaat
     3541 aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat atctgcgaga
     3601 cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt cgattttttg
     3661 aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt caatctcatg
     3721 agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa gtttgacgat
     3781 atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga ttagttgatg
     3841 ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg aaagtttggt
     3901 cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat ctaaagcaac
     3961 ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga cgttgtctag
     4021 agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat catttttacg
     4081 ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct ttccttcttt
     4141 tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac cacttgctcc
     4201 taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat aaagcgcatc
     4261 ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat tcctccttta
     4321 gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta aaaagacaga
     4381 aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag gtttgacatt
     4441 gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg ttccttgttg
     4501 accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac cgccaggagc
     4561 ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc ccacggcatt
     4621 cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac aaatagccac
     4681 ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata gcaagacgcc
     4741 taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta tgataatacc
     4801 ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt ttaaaggttc
     4861 caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg tgtcgatcag
     4921 cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag tgtaaatagc
     4981 agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag tgtaagaagt
     5041 gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc cgcttgtttc
     5101 gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat cttcagtact
     5161 cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag tagaatcaat
     5221 gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg tgatttttgc
     5281 tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga tttgaaagtc
     5341 tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc ctgcatcagt
     5401 agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc catcttttgc
     5461 catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt cagctatttt
     5521 ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg gacgttcttc
     5581 agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt ctcgattggc
     5641 acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt cttctgcagc
     5701 gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca cggcaaacaa
     5761 taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca taaaattcat
     5821 attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta aagctagctt
     5881 aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa aagtcactat
     5941 agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac gaagaagaaa
     6001 tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac gtgcatagtt
     6061 gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa tggggaacag
     6121 ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat tttgttaatg
     6181 aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc tggtaatttt
     6241 cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat tggaatggct
     6301 catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat aagaagaggt
     6361 gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa atggaaaaaa
     6421 catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc taaaatatcc
     6481 tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt tagaagaaaa
     6541 cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga tcggggcact
     6601 tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga cgatcgagtt
     6661 agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt tccatgtggt
     6721 agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc gtaccactca
     6781 tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta tcgtttatac
     6841 gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt atgcacgatt
     6901 ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa atgcagaaaa
     6961 tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata cgatcaaata
     7021 ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag tgcaacaagt
     7081 attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag atttcgaacg
     7141 aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag atctgcttgt
     7201 tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag aaactggccg
     7261 aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc gtaatgtcaa
     7321 gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga tggaaaccat
     7381 cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt tgatctctga
     7441 gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga tcaatttgat
     7501 gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg ctcatgcaga
     7561 tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac gcggagacgt
     7621 gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag aaaatgtaat
     7681 gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc gaaaaatctt
     7741 gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa agaaaatcat
     7801 ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc gtgatctgat
     7861 gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa gcaatgattt
     7921 tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact tatttgaaca
     7981 aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac gtcgaaataa
     8041 aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg ctcttttttt
     8101 gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac gatagaataa
     8161 agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag aacaaaagat
     8221 cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag aagaactaca
     8281 aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag gaaacaaaac
     8341 tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa aaacgagaca
     8401 agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa tgcatacagg
     8461 atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg attattgttt
     8521 tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg gtgaaatcgg
     8581 gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta tttacgctga
     8641 tttaattagt taagggacga gaagagaagc taagcagagc agagacctca aacttgagat
     8701 tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt tgtacatact
     8761 gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga tgtgcaagtg
     8821 atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa tatgaaaatt
     8881 acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg cgtgatgatt
     8941 gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt aaagacagat
     9001 tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc tattgcaaaa
     9061 gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa cagcagagga
     9121 gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg cgtggtaaaa
     9181 ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca gtatatgggc
     9241 gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc aggggatact
     9301 gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt cgctttcttg
     9361 ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc gcaaacaatt
     9421 cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga acaagatccc
     9481 gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt ctcggaaacg
     9541 ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat atattgaaag
     9601 tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga atgtcagtga
     9661 aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt caacaattca
     9721 acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac gtttgacatt
     9781 tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag gattagacaa
     9841 ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac gctatattac
     9901 gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag cattggaaga
     9961 tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg caaagctagc
    10021 tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag tcgcaaccat
    10081 gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg tcagtgatat
    10141 ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt ctgattatca
    10201 gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc atcatcgttt
    10261 gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga tcatcgatcg
    10321 ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct ctgttagcca
    10381 tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact tgacattgga
    10441 aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc gacaaccgca
    10501 gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg atactgaccg
    10561 tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac aaatggtcaa
    10621 tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg tcgcacaaaa
    10681 aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc tacattattt
    10741 gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg aaatcggcag
    10801 acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt ccaaagcaat
    10861 cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac agtacagcaa
    10921 gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca tttgcgccct
    10981 gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga aagtttccca
    11041 gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt agcagaaatg
    11101 caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga tacagctaat
    11161 tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa aatcgaccat
    11221 catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc aagtagctgt
    11281 agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat gaacgttaag
    11341 gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt gtatccctcc
    11401 acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt tgatgcaagt
    11461 gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc cggttatctt
    11521 tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc gcaagaaatt
    11581 cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact tcctggagtg
    11641 attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta ttaccgcgta
    11701 cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca tggaggaggc
    11761 catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga aatctataaa
    11821 gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata cttcgttcga
    11881 aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc tttttccgtt
    11941 acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca aattgcccag
    12001 aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca gaatgcggga
    12061 acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg gtattagatg
    12121 caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac ttgaaagtaa
    12181 aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt ttagtagaag
    12241 gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg aaatcggaat
    12301 ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct actactaatg
    12361 ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga atgtatggtt
    12421 ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga attgcgtcag
    12481 cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac aatgtgactg
    12541 gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc ggtgttcaac
    12601 aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg agtttgtctc
    12661 aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat gaatgcggac
    12721 atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca cttgttccag
    12781 cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta ttgcttagct
    12841 ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta cttttccaat
    12901 tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt ttgagtgaag
    12961 gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt gcagcagcac
    13021 tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg atcttattgt
    13081 tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt tgatctgagc
    13141 taataccgag agttagctga ttgaatacca tttatttgga ataagggctc tttgtcaata
    13201 aggactgatg ggttgtacaa aatcaaatct gggtcagaat gaacctcatt ctggctcag
//
LOCUS       DAYYOA010000075        13209 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000075 DAYYOA010000000
VERSION     DAYYOA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.075"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1013
                     /locus_tag="ACHBKA_001909"
     CDS             144..1013
                     /locus_tag="ACHBKA_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9445584.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1222..2247
                     /locus_tag="ACHBKA_001910"
     CDS             1222..2247
                     /locus_tag="ACHBKA_001910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9445585.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2286..3236
                     /gene="manA"
                     /locus_tag="ACHBKA_001911"
     CDS             2286..3236
                     /gene="manA"
                     /locus_tag="ACHBKA_001911"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM9445586.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3330..5033
                     /locus_tag="ACHBKA_001912"
     CDS             3330..5033
                     /locus_tag="ACHBKA_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9445587.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5035..7068
                     /locus_tag="ACHBKA_001913"
     CDS             5035..7068
                     /locus_tag="ACHBKA_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM9445588.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7079..7519
                     /locus_tag="ACHBKA_001914"
     CDS             7079..7519
                     /locus_tag="ACHBKA_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9445589.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7719..8846)
                     /locus_tag="ACHBKA_001915"
     CDS             complement(7719..8846)
                     /locus_tag="ACHBKA_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9445590.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8849..9526)
                     /locus_tag="ACHBKA_001916"
     CDS             complement(8849..9526)
                     /locus_tag="ACHBKA_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9445591.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9790..11556
                     /locus_tag="ACHBKA_001917"
     CDS             9790..11556
                     /locus_tag="ACHBKA_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM9445592.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11571..12008
                     /locus_tag="ACHBKA_001918"
     CDS             11571..12008
                     /locus_tag="ACHBKA_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9445593.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12021..13172
                     /locus_tag="ACHBKA_001919"
     CDS             12021..13172
                     /locus_tag="ACHBKA_001919"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM9445594.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3697 t
ORIGIN      
        1 ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt tctcacattt
       61 tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta aaataaagct
      121 tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc acctatttaa
      181 aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac aagaacgttg
      241 ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca gaagaagctg
      301 gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg ggggtgatca
      361 aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca gatatccttt
      421 gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat ggacgtatgg
      481 ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa gagaataaga
      541 acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga aaatctgaag
      601 aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta aatgttacta
      661 acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta cataatgaag
      721 aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca gaagctagcg
      781 gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg aaattcatga
      841 tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca gttgatttct
      901 tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc gaatggtggt
      961 tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct taactttttc
     1021 tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat cgcttattct
     1081 agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg cgcagttttt
     1141 ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg taagaatctg
     1201 atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt gaaaaagatg
     1261 gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca agtagaaaca
     1321 tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga agatctgttg
     1381 tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt acgtctatta
     1441 gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga atttgaaaat
     1501 cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac atggatacca
     1561 aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct agaaccaggt
     1621 gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct tgggttgatg
     1681 ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt attggaacca
     1741 ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt tcccacagaa
     1801 caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc aagagttttg
     1861 gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat tacttttgtc
     1921 caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga agagacgatt
     1981 gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt aggatgggac
     2041 ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta tacaggtacg
     2101 tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa tcgcgtacat
     2161 cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga aaactttttg
     2221 aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg agaggagttt
     2281 gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt tggggaggaa
     2341 gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga gaagattggg
     2401 cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc aaagggaaaa
     2461 gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca gaaccagtgt
     2521 ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa gtacatccag
     2581 atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc tggtatatca
     2641 tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca agagaagaac
     2701 tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta ccagttaaaa
     2761 aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa ggaatcatga
     2821 ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac gacagaaaag
     2881 atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta acaactgtcc
     2941 ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca tcgatcgtca
     3001 cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga atcacatctt
     3061 tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga gaccttgtcg
     3121 ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca aatggcgttt
     3181 ctgagtgggc ggtccaaggt gaactagcaa tcattgcgac tgaaccaggc aaatagttcc
     3241 cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat aaaatccgtc
     3301 catcaataaa tagaagtatg gtgaaaaaga tggaatacaa atggaaagca aacaacagca
     3361 gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa atgatcatgc
     3421 caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa ggtgtgtctg
     3481 gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg cttttaccca
     3541 tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca gtcgccggtg
     3601 caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta ggagcgatga
     3661 ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc cacgtgaaat
     3721 tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact ggtagcgtta
     3781 tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac ttgttgctcg
     3841 cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt ctgatcttag
     3901 aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg acacctttag
     3961 gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag gcaaatcctg
     4021 gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag gaaaaagtaa
     4081 atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc tattttccat
     4141 ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca agcggcacgt
     4201 tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg tcactactcg
     4261 tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg atagcaatca
     4321 gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat aaagaaaaag
     4381 agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt cattccgtca
     4441 tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg ctgaaaaaga
     4501 aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat gaattgaata
     4561 accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca agagaaaaag
     4621 tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc ttgctgtcac
     4681 gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata gcagatatca
     4741 cagaacaaaa aaagtcgcct gctaatattg tatttttgta taaaaacaac agacgcggct
     4801 cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa aaacagtttg
     4861 ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt tatatcgcac
     4921 aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta gttctagttg
     4981 aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata taagatgtat
     5041 ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc acctgtatct
     5101 atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg cgaattagat
     5161 aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc tagaggctca
     5221 tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac agatgggaaa
     5281 agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt gttactgacg
     5341 acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa taccactttt
     5401 tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga aatcattcgg
     5461 aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac agcgaatgta
     5521 ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga tgcatcactg
     5581 aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa agtaatcggg
     5641 gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca tttggttttg
     5701 atgctgacat tgacgatgga gcgtgtgact aaaaatcatt tactgacaaa tgagacatat
     5761 aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc acggatcgct
     5821 tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg attattaaat
     5881 gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac acagcttgct
     5941 tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca tttttatgaa
     6001 gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc aagagccgtt
     6061 ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca atacgaggaa
     6121 gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa attatccgaa
     6181 gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag tccgaaagaa
     6241 agcaatatca gtattgcagg attttcgcca agtggattag cttctactag catgttggaa
     6301 ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg gattgctgat
     6361 ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc tattttacca
     6421 ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga agtcaagcag
     6481 ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac gtataacagc
     6541 aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat ggatcaaatc
     6601 aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac gatttccgaa
     6661 acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga agaggtggtt
     6721 catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca tacaaatatg
     6781 gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat tttcaatttg
     6841 aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc ccgtatgtta
     6901 gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg aaagatcagt
     6961 ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa taaagaaatc
     7021 gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag gtgaaaagat
     7081 gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt ggaaagaagc
     7141 gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg catatatcca
     7201 atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg tagccttgcc
     7261 acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag tccaagtgcc
     7321 tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct tatttgtagt
     7381 ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt tctgttcaga
     7441 catcgaccaa gtaatggcgc tcagtgatgc gaaaaccatt gaagaagtcc aaatgatttt
     7501 tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc atcaagtaat
     7561 aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct tattactgcg
     7621 gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat aggggacggt
     7681 gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt ttttatatag
     7741 atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt caagatacca
     7801 ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc tatgatcaca
     7861 ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat ccatccaatc
     7921 gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa aagattgact
     7981 agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa gagcacccac
     8041 aacaacagga acagaaagct ctggaaaaga tactcataca tcggaaggat tcgtatctcc
     8101 tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt gattattact
     8161 agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc ataataaatg
     8221 gtaaaacgga tgctatgttt ctttaccata ctatccggta atagattgac tagagagatt
     8281 acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt tatacataaa
     8341 atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc taaagggaga
     8401 tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat ataacggagc
     8461 aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc agaaatcagt
     8521 cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg gacattccct
     8581 gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa actttccaca
     8641 aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat ctcttcatag
     8701 ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa aagtgggaat
     8761 gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt tagtaagaga
     8821 ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga taaatagcta
     8881 aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga aaacatgctt
     8941 tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc gtcttcccct
     9001 ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg tctaatccgt
     9061 tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca gcaagcaaga
     9121 catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac tcagtcatct
     9181 tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat ttgatcaaag
     9241 tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct agaaaaaaga
     9301 aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac aattgatcat
     9361 aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca aatccgttcg
     9421 gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt cgaatcacac
     9481 gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat aggcctccaa
     9541 tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta acaaaagtta
     9601 ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga agttttctga
     9661 taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt ttcgtgccaa
     9721 agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa ggagagaggg
     9781 agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc gagggtacaa
     9841 aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact gattgcctgg
     9901 ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc tttggctacc
     9961 atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac tggcggaaaa
    10021 gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg tgtgattgtc
    10081 ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg tggatatgcc
    10141 atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga aatgctagtc
    10201 aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt ttctgcgatt
    10261 ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat cattttatct
    10321 gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt atttttgaat
    10381 aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc agaggctgga
    10441 aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt attacttgct
    10501 tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat gatcattcac
    10561 tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc gttattattc
    10621 ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat ggatgctgga
    10681 ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac accgttcaat
    10741 tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt tggtatttct
    10801 gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa atctgttcaa
    10861 gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca aggagctaca
    10921 ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc aagtgcgatg
    10981 ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac ggtgaccaat
    11041 caagcaatca gccaactgac agatgactcg aatacattga tcgttactca ggtcgaacta
    11101 caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga aaactttttg
    11161 aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa aacatcagtt
    11221 tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa acaaatcaaa
    11281 gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat gaccgtgctc
    11341 aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa tatccaagat
    11401 ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga aaaagtacag
    11461 atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc gcaaaaatac
    11521 gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc atgcaaagtt
    11581 tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa gcgatccgct
    11641 attgcggtca gatgcttgta gaagcagggt gcgttgatga ggaatatatc gatgccatgg
    11701 tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt cctcatggaa
    11761 cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa gtaccagatg
    11821 gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc ggtattgcag
    11881 gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc agtgatatgg
    11941 acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa aatctagcaa
    12001 ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat atcggtcgag
    12061 gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta gatgtgaatc
    12121 acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta gcagatgaat
    12181 caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca aatccagaaa
    12241 aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc ccgaatatct
    12301 taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag gaaggaagca
    12361 caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca tttctagaaa
    12421 aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc ggctttccaa
    12481 atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta ttcgtccaag
    12541 tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa aaaatccaat
    12601 taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa ctatttagtg
    12661 tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac cagaccattg
    12721 atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg caggaaacag
    12781 gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg tatattgaaa
    12841 aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt gtcgctcgga
    12901 caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga gaactacaag
    12961 aacataactt gacttgccca catttgacgg caacaatggg gattattttt aattactatg
    13021 atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa aacttggaac
    13081 aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat atcaagcaaa
    13141 acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg ggaatgtgac
    13201 gaaattttt
//
LOCUS       DAYYOA010000076        12775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000076 DAYYOA010000000
VERSION     DAYYOA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.076"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..639)
                     /locus_tag="ACHBKA_001920"
     CDS             complement(277..639)
                     /locus_tag="ACHBKA_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445595.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(713..1093)
                     /locus_tag="ACHBKA_001921"
     CDS             complement(713..1093)
                     /locus_tag="ACHBKA_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM9445596.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1175..2350
                     /locus_tag="ACHBKA_001922"
     CDS             1175..2350
                     /locus_tag="ACHBKA_001922"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM9445597.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2427..3011)
                     /locus_tag="ACHBKA_001923"
     CDS             complement(2427..3011)
                     /locus_tag="ACHBKA_001923"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9445598.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3212..4210
                     /locus_tag="ACHBKA_001924"
     CDS             3212..4210
                     /locus_tag="ACHBKA_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9445599.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4322..4831
                     /locus_tag="ACHBKA_001925"
     CDS             4322..4831
                     /locus_tag="ACHBKA_001925"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM9445600.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4861..5520)
                     /locus_tag="ACHBKA_001926"
     CDS             complement(4861..5520)
                     /locus_tag="ACHBKA_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM9445601.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5522..6286)
                     /locus_tag="ACHBKA_001927"
     CDS             complement(5522..6286)
                     /locus_tag="ACHBKA_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM9445602.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6294..6971)
                     /locus_tag="ACHBKA_001928"
     CDS             complement(6294..6971)
                     /locus_tag="ACHBKA_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM9445603.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7008..8393)
                     /locus_tag="ACHBKA_001929"
     CDS             complement(7008..8393)
                     /locus_tag="ACHBKA_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM9445604.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8603..8687)
                     /gene="ffs"
                     /locus_tag="ACHBKA_001930"
     ncRNA           complement(8603..8687)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHBKA_001930"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACHBKA_001931"
     CDS             complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACHBKA_001931"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM9445605.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9332..10189
                     /locus_tag="ACHBKA_001932"
     CDS             9332..10189
                     /locus_tag="ACHBKA_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445606.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10270..10605)
                     /locus_tag="ACHBKA_001933"
     CDS             complement(10270..10605)
                     /locus_tag="ACHBKA_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445607.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10672..12042)
                     /locus_tag="ACHBKA_001934"
     CDS             complement(10672..12042)
                     /locus_tag="ACHBKA_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM9445608.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12120..12656)
                     /locus_tag="ACHBKA_001935"
     CDS             complement(12120..12656)
                     /locus_tag="ACHBKA_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9445609.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3808 a         2590 c         2122 g         4255 t
ORIGIN      
        1 tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat
       61 ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta
      121 ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact
      181 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
      241 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      301 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      361 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      421 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      481 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      541 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      601 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      661 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      721 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      781 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      841 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      901 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      961 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
     1021 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
     1081 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
     1141 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1201 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1261 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1321 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1381 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1441 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1501 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1561 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1621 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1681 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1741 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1801 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1861 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1921 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1981 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     2041 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     2101 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     2161 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2221 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2281 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2341 aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt
     2401 tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca
     2461 tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc
     2521 atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca
     2581 cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata
     2641 ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct
     2701 gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca
     2761 aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg
     2821 atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa
     2881 agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg
     2941 tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt
     3001 gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata
     3061 cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt
     3121 ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat
     3181 tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg
     3241 tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa
     3301 aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata
     3361 tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga
     3421 aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac
     3481 tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt
     3541 cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga
     3601 atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa
     3661 aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc
     3721 tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag
     3781 tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt
     3841 atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac
     3901 gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca
     3961 tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc
     4021 tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa
     4081 actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat
     4141 taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg
     4201 tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc
     4261 atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa
     4321 tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg
     4381 cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga
     4441 tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca
     4501 taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag
     4561 tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc
     4621 cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa
     4681 ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt
     4741 cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga
     4801 accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat
     4861 ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac
     4921 agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc
     4981 tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa
     5041 aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa
     5101 aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt
     5161 cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata
     5221 atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg
     5281 gaaatctggc aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc
     5341 aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc
     5401 gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag
     5461 tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat
     5521 attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag
     5581 gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta
     5641 tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca
     5701 gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg
     5761 gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc
     5821 cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa
     5881 tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag cctaccacta
     5941 catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc
     6001 ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg
     6061 ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta
     6121 gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta
     6181 attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac
     6241 gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct
     6301 tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt
     6361 ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt
     6421 ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt
     6481 ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa
     6541 tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga
     6601 tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa
     6661 ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc
     6721 ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc
     6781 aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc
     6841 tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca
     6901 gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt
     6961 tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg
     7021 tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca
     7081 ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta
     7141 gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta
     7201 atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc
     7261 attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag
     7321 cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca
     7381 gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg
     7441 tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata
     7501 aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt
     7561 tcttttaata attcatgccc acgttctaga taactattta tttcttgttc atcttctgta
     7621 aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct
     7681 tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt
     7741 tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata
     7801 acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc
     7861 atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca
     7921 taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat
     7981 gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat
     8041 gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca
     8101 tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc
     8161 gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct
     8221 tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt
     8281 ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa
     8341 ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct
     8401 ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc
     8461 tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag
     8521 tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac
     8581 ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc
     8641 ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa
     8701 tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg
     8761 gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc
     8821 tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt
     8881 tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc
     8941 gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca
     9001 gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc
     9061 ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac
     9121 tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg
     9181 catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc
     9241 tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa cttttttttc
     9301 tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta
     9361 cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat
     9421 tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt
     9481 gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc gctatctttt
     9541 taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc acgtcgaatc
     9601 aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat ttcgctattc
     9661 tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa taaaccgtga
     9721 ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg attttgatac
     9781 gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg tccctttgtt
     9841 tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc acagccgtac
     9901 tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa aagatagtaa
     9961 gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga tcattcaaga
    10021 attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc ttcatcgtaa
    10081 tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc taaaaaaagc
    10141 caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa gatagattaa
    10201 aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt gttcaaaagc
    10261 tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta catcttcggt
    10321 tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt tgatttttgc
    10381 taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga tttctagtaa
    10441 tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc gagtagtcat
    10501 atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta cactggcagc
    10561 ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta tcagctcctt
    10621 tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca ctcagtgaaa
    10681 tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc caaatccgac
    10741 aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt gaaatgtttc
    10801 tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta tattctctat
    10861 cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt tgaaatctcc
    10921 taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta actcaggaac
    10981 ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat gaaaatcatt
    11041 tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac ttttttttcg
    11101 tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg caacaaaatg
    11161 tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac cagctacgct
    11221 tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct caatcgttac
    11281 tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg caaacgtaca
    11341 agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag acaaaatctc
    11401 ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta ttgcatttgg
    11461 atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc gatcaaaaaa
    11521 gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg cgttcgaaac
    11581 tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga tctcattcgt
    11641 cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac caccgggagc
    11701 cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc caacgatcat
    11761 tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact ttccattgac
    11821 agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc tatggtaagt
    11881 ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt ttttcacgct
    11941 tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa gacgatattt
    12001 tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt ttcgttagac
    12061 attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca tgttgctgcc
    12121 taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc gtttttatcc
    12181 caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg caaacttccc
    12241 aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt gtgacctttg
    12301 acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga ttttgcaatg
    12361 atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac aatgcttaaa
    12421 acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat ttcttctaat
    12481 atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta tgtagaacaa
    12541 ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc ttccacgtat
    12601 accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc catcattctt
    12661 acctccgtct cttttcatct acccataaat aaataacata aataattcta aaaatattat
    12721 tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc cgcct
//
LOCUS       DAYYOA010000077        12734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000077 DAYYOA010000000
VERSION     DAYYOA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.077"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHBKA_001936"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHBKA_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9445610.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHBKA_001937"
     CDS             complement(322..891)
                     /locus_tag="ACHBKA_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM9445611.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHBKA_001938"
     CDS             1077..1979
                     /locus_tag="ACHBKA_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9445612.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHBKA_001939"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHBKA_001939"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM9445613.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHBKA_001940"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHBKA_001940"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM9445614.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHBKA_001941"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHBKA_001941"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM9445615.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHBKA_001942"
     CDS             8547..8732
                     /locus_tag="ACHBKA_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM9445616.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHBKA_001943"
     CDS             complement(8794..9990)
                     /locus_tag="ACHBKA_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9445617.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHBKA_001944"
     CDS             complement(10160..11596)
                     /locus_tag="ACHBKA_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9445618.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHBKA_001945"
     CDS             11889..12698
                     /locus_tag="ACHBKA_001945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9445619.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAYYOA010000078        12691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000078 DAYYOA010000000
VERSION     DAYYOA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.078"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHBKA_001946"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHBKA_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM9445620.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHBKA_001947"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHBKA_001947"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM9445621.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACHBKA_001948"
     CDS             complement(2303..2965)
                     /locus_tag="ACHBKA_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9445622.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACHBKA_001949"
     CDS             3149..4795
                     /locus_tag="ACHBKA_001949"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9445623.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHBKA_001950"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHBKA_001950"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9445624.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="ACHBKA_001951"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="ACHBKA_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="ACHBKA_001952"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="ACHBKA_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="ACHBKA_001953"
     CDS             complement(8944..10023)
                     /locus_tag="ACHBKA_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9445625.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="ACHBKA_001954"
     CDS             complement(10126..10566)
                     /locus_tag="ACHBKA_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9445626.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="ACHBKA_001955"
     CDS             complement(10623..11033)
                     /locus_tag="ACHBKA_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9445627.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="ACHBKA_001956"
     CDS             complement(11213..11647)
                     /locus_tag="ACHBKA_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9445628.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="ACHBKA_001957"
     CDS             complement(11747..12595)
                     /locus_tag="ACHBKA_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9445629.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4247 a         2358 c         2246 g         3840 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag t
//
LOCUS       DAYYOA010000079        12190 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000079 DAYYOA010000000
VERSION     DAYYOA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.079"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..316)
                     /locus_tag="ACHBKA_001958"
     CDS             complement(152..316)
                     /locus_tag="ACHBKA_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445630.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            611..1939
                     /locus_tag="ACHBKA_001959"
     CDS             611..1939
                     /locus_tag="ACHBKA_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM9445631.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2141..2974
                     /locus_tag="ACHBKA_001960"
     CDS             2141..2974
                     /locus_tag="ACHBKA_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM9445632.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2990..3727
                     /locus_tag="ACHBKA_001961"
     CDS             2990..3727
                     /locus_tag="ACHBKA_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM9445633.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3882..4850)
                     /locus_tag="ACHBKA_001962"
     CDS             complement(3882..4850)
                     /locus_tag="ACHBKA_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM9445634.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5082..5915
                     /locus_tag="ACHBKA_001963"
     CDS             5082..5915
                     /locus_tag="ACHBKA_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM9445635.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5949..6788
                     /locus_tag="ACHBKA_001964"
     CDS             5949..6788
                     /locus_tag="ACHBKA_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9445636.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6814..7374
                     /locus_tag="ACHBKA_001965"
     CDS             6814..7374
                     /locus_tag="ACHBKA_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9445637.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7573..9294
                     /locus_tag="ACHBKA_001966"
     CDS             7573..9294
                     /locus_tag="ACHBKA_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM9445638.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9459..10601
                     /locus_tag="ACHBKA_001967"
     CDS             9459..10601
                     /locus_tag="ACHBKA_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9445639.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10617..11828
                     /gene="thiI"
                     /locus_tag="ACHBKA_001968"
     CDS             10617..11828
                     /gene="thiI"
                     /locus_tag="ACHBKA_001968"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM9445640.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4153 a         2047 c         2531 g         3459 t
ORIGIN      
        1 tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt
       61 ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc
      121 tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata
      181 cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga
      241 cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag
      301 caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa
      361 tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac
      421 aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt
      481 atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata
      541 gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa
      601 ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca
      661 agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc
      721 tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac
      781 ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac
      841 attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta
      901 cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac
      961 aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga
     1021 tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc
     1081 ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa
     1141 attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga
     1201 cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc
     1261 attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca
     1321 tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga
     1381 tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac
     1441 agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat
     1501 ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg
     1561 ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc
     1621 aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg
     1681 agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc
     1741 aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt
     1801 tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt
     1861 gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc
     1921 aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga
     1981 gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg
     2041 tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg
     2101 atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt
     2161 atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac
     2221 ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa
     2281 aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc
     2341 ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga
     2401 gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac
     2461 agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc
     2521 ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt
     2581 ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga
     2641 acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct
     2701 gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt
     2761 ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc
     2821 ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact
     2881 gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg
     2941 ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa
     3001 atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt
     3061 tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag
     3121 aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga
     3181 gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta
     3241 caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt
     3301 caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa
     3361 tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa
     3421 aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga
     3481 tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata
     3541 tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga
     3601 gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac
     3661 aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa
     3721 taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg
     3781 aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt
     3841 cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa
     3901 atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt
     3961 tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt tcccccgcct
     4021 tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg
     4081 attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca
     4141 cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc
     4201 ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc
     4261 agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg
     4321 atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg
     4381 tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct
     4441 tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca
     4501 ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca
     4561 agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata
     4621 cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg
     4681 tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg
     4741 atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt
     4801 tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact
     4861 tccttacgaa taattttgca tagccgttaa atcttattgt atactatata aaagagtttt
     4921 gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa
     4981 tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac
     5041 aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa
     5101 catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg
     5161 cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct
     5221 cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc
     5281 aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg
     5341 cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag
     5401 tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta
     5461 gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg
     5521 atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca
     5581 tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga
     5641 actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag
     5701 agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac
     5761 atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt
     5821 ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag
     5881 atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg
     5941 cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt
     6001 caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat
     6061 gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat
     6121 tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg
     6181 accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc
     6241 gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac
     6301 aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga
     6361 tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag
     6421 agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa
     6481 agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata
     6541 tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa
     6601 ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag
     6661 gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact
     6721 ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga
     6781 tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa
     6841 gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca
     6901 gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca
     6961 gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta
     7021 gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa
     7081 caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc attactagga
     7141 atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca
     7201 gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag
     7261 atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg
     7321 attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag
     7381 tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt
     7441 gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat
     7501 tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg
     7561 gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc
     7621 gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat
     7681 gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat
     7741 gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa
     7801 tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa
     7861 aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct
     7921 acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg
     7981 gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg
     8041 aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa
     8101 cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac
     8161 cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct
     8221 ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt
     8281 gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa
     8341 gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt
     8401 gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat
     8461 tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa
     8521 tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt attgattgag
     8581 ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt
     8641 ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt
     8701 caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta
     8761 gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc
     8821 gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt
     8881 tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt
     8941 gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa
     9001 gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca
     9061 catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat
     9121 cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa
     9181 gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct
     9241 ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa
     9301 aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta
     9361 tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg
     9421 ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg
     9481 caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta
     9541 ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag
     9601 caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag
     9661 gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg
     9721 ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat
     9781 taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg
     9841 tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga
     9901 ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc
     9961 ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat
    10021 ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg
    10081 gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag
    10141 ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta
    10201 aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa
    10261 aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa
    10321 ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg
    10381 tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta
    10441 aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg
    10501 ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta
    10561 ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga
    10621 aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa
    10681 gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca
    10741 aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag
    10801 ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag
    10861 aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg
    10921 ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta
    10981 atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc
    11041 aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat
    11101 cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc
    11161 ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag
    11221 tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa
    11281 aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag
    11341 tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga
    11401 caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt
    11461 tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg
    11521 tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac
    11581 ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg
    11641 aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg
    11701 tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct
    11761 tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat
    11821 tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa
    11881 aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc
    11941 atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta
    12001 tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc
    12061 gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt
    12121 tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta
    12181 tcagaggttg
//
LOCUS       DAYYOA010000080        12042 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000080 DAYYOA010000000
VERSION     DAYYOA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.080"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            452..721
                     /gene="rpsN"
                     /locus_tag="ACHBKA_001969"
     CDS             452..721
                     /gene="rpsN"
                     /locus_tag="ACHBKA_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9445641.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            736..915
                     /gene="rpmF"
                     /locus_tag="ACHBKA_001970"
     CDS             736..915
                     /gene="rpmF"
                     /locus_tag="ACHBKA_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9445642.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            947..1324
                     /locus_tag="ACHBKA_001971"
     CDS             947..1324
                     /locus_tag="ACHBKA_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445643.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1345..1494
                     /gene="rpmG"
                     /locus_tag="ACHBKA_001972"
     CDS             1345..1494
                     /gene="rpmG"
                     /locus_tag="ACHBKA_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9445644.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1518..2501
                     /locus_tag="ACHBKA_001973"
     CDS             1518..2501
                     /locus_tag="ACHBKA_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9445645.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2531..2653
                     /locus_tag="ACHBKA_001974"
     CDS             2531..2653
                     /locus_tag="ACHBKA_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9445646.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2671..4194
                     /locus_tag="ACHBKA_001975"
     CDS             2671..4194
                     /locus_tag="ACHBKA_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9445647.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4218..4457
                     /locus_tag="ACHBKA_001976"
     CDS             4218..4457
                     /locus_tag="ACHBKA_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445648.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4650..5111)
                     /locus_tag="ACHBKA_001977"
     CDS             complement(4650..5111)
                     /locus_tag="ACHBKA_001977"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9445649.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5306..5524)
                     /locus_tag="ACHBKA_001978"
     CDS             complement(5306..5524)
                     /locus_tag="ACHBKA_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445650.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5642..7258
                     /locus_tag="ACHBKA_001979"
     CDS             5642..7258
                     /locus_tag="ACHBKA_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9445651.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7499..8839)
                     /locus_tag="ACHBKA_001980"
                     /pseudo
     CDS             complement(7499..8839)
                     /locus_tag="ACHBKA_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9277..9423)
                     /locus_tag="ACHBKA_001981"
     CDS             complement(9277..9423)
                     /locus_tag="ACHBKA_001981"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445652.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9462..9656)
                     /locus_tag="ACHBKA_001982"
     CDS             complement(9462..9656)
                     /locus_tag="ACHBKA_001982"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9445653.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9566..9898)
                     /locus_tag="ACHBKA_001983"
     CDS             complement(9566..9898)
                     /locus_tag="ACHBKA_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9445654.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10412..10603)
                     /locus_tag="ACHBKA_001984"
     CDS             complement(10412..10603)
                     /locus_tag="ACHBKA_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445655.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10697..11083)
                     /locus_tag="ACHBKA_001985"
     CDS             complement(10697..11083)
                     /locus_tag="ACHBKA_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9445656.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            11777..>12042
                     /locus_tag="ACHBKA_001986"
     CDS             11777..>12042
                     /locus_tag="ACHBKA_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM9445657.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRAN"
BASE COUNT         4032 a         1862 c         2286 g         3862 t
ORIGIN      
        1 attaaacagc atctaatttt gaatacacga aagaatcagt atgattgaat tagttcactt
       61 aatgtctatt cttataatat aaggaataat caaagtttta aaaaattggc gataaaccag
      121 ttaaacataa aagattgaat aaaaagttat agtttctttc atattgccgt tttaagtaga
      181 ggaaaagcta cttaaaacgg tatttgtgtt tgattggact aataaaagtg aattcttatt
      241 tttcagagcc taaactgcat attcttttaa atgatttgat tatctctatg taccgataaa
      301 ttcgttttca aatagttatc tattcatatg agttttattt catgttattt attgttgaca
      361 agagcaactc ttgttggtaa agtatcagtg tcaaaacgta acgattacga tttgaggtgt
      421 ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa ttgctaaagc
      481 aaagaaacaa atggcgatga ttgaaaagta tgcagataaa cgtcaggaat taaaagccgc
      541 aggagatcgc accgctctcg ctaaattgcc aagagattca aatccgaatc gtctgaggct
      601 tcgtgatcag acggatggtc gtccaagagg gtatatgcga aagttcggta tgtcccgtat
      661 taagtttaga gaacttgccc atcaagggtt gattcctgga gtaaaaaaag caagttggta
      721 aaaggaggaa atatcatggc agtaccagca agaaaaacat ctaaggcgaa gaaacggtta
      781 agaagaactc atcaaactgt agtaaaacca gaaatttcat ttgacgaagc gaatggtgat
      841 tatagacgta gccatcatgt atctttaaaa gggtactata atggaaagaa agtaattaaa
      901 ggagcttcaa aatagtttaa tatataactt ttaggaggga atcaaaatgg aaattattta
      961 cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc aatcgctaga
     1021 taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa cgggtttacc
     1081 gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc aaggcttatc
     1141 atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg agttatttca
     1201 atgtatcgat ggttattggg agatacctgt tgatatgaaa gaaaatttag tttcacaatt
     1261 ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc ttgaagatcg
     1321 ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg tgtcgaaact
     1381 ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg gttagaatta
     1441 aaaaagtatt cgccaaaatt gcgcagacgg gcaattttta aagaagtgaa atagctaagt
     1501 ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt ttaggggctg
     1561 gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg gaagaaatta
     1621 ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta gttcatagcg
     1681 aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc agtgatttat
     1741 tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca atttcacaaa
     1801 tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca cttttaacag
     1861 cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt gatagtgaca
     1921 gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg gcagatcgca
     1981 tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta cagagagaaa
     2041 tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa acgattcaaa
     2101 aaaaggattt ttataatttg aaaaaattta atcatccctt agtttttgaa agtgattcat
     2161 cagaaaatca ctataatggc aaatactatc atcaacacca tcaagtacat gagtttaaat
     2221 caattctctt aacttcagat caagacataa aaaaagattt tttactccaa tggattgact
     2281 ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt gctttacaag
     2341 agcaggattt tatagttgcc atgcaagagg taaacgaaca ggttaatttt caatacacca
     2401 ctcgacaaaa agaccaaacc actattattc taatcggaaa aatgttggat gaagaaagga
     2461 tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta gtgattgggg
     2521 gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta aaagcccgaa
     2581 tattaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa ataaaatgaa
     2641 gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg acttattggg
     2701 atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa agtatcgaat
     2761 gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga tttcaccaaa
     2821 aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc agaacctcat
     2881 gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt ttttgtatat
     2941 cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa gaaagctggg
     3001 cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga agaagaagat
     3061 catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg ggtttcaccc
     3121 aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt gtatccgaaa
     3181 aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa acggttggat
     3241 gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac gcaacacgca
     3301 gcctttggat atttagcttt ggattatgga ttgaaacaag ttcctattgc tggactaagt
     3361 cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt taaaaaaaat
     3421 aagatcaatt atatttactt tgaaaagaat gctaatgata aaattgcaaa aactttggcc
     3481 aacgaagctg ggataaaatt ggaagtactg aatcctcttg aaagtcttac aaaagaacaa
     3541 atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc gttagaaaaa
     3601 acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga tgaaaagaca
     3661 gttgctaatg ggtactttaa agatgccgat gtgaaggatc gcgaattgag tgattataca
     3721 ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga agttttcgat
     3781 tacaaagcaa aaatcaataa agacatgaca gctgccgagt acaaagatta ttatactact
     3841 ggctataaga cagatattga tactatcaac attaaagaca acaccattga ttttgtagtc
     3901 aacggtgaac atcatcaata cacatacaaa tatgttggat acaaaatttt gaattatgaa
     3961 aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc tggtcgtttt
     4021 aaatatattc aatttagtga tcatggaatt gcaccaagta aggcagagca tttccatatt
     4081 ttctttggtg gagaaagtca agaaaaactc tataatgaat tgcataattg gcctactttc
     4141 tatccagctt cattgtctaa acatgaaatt gctcaagaaa tgatggctca ttaaaattca
     4201 atttttggag gaaatacatg gtaagaaaag tattattgtc tatggattcc aagaatattg
     4261 atgcttatcg gcgttatcga tttgcaaagc aaatgagatt aaatgggagt acattagctg
     4321 atattgctgc aactttacat gttagtattg acacagttca tagagtatta aaacgggatc
     4381 ctgattgttg ttttatcaag gtcaatactg gaagaagtag acgtttagca aaacggcata
     4441 atagcttggc caattaattg ataataccag agtagatcat ttttccctta tttggatttg
     4501 atctgctttt tattatgtct tgaattgtca aatcaagcgc aacactttat caaagaagac
     4561 ttcataaggt gttctccaat ctaaacactt tctaggtctg ttattcagtt tttcaatcat
     4621 ctgatttatc gttgagttat ctaccgtagc tatctctttc gattttggca agtattctcg
     4681 aatgagtcta ttcgtatttt aatttgttca ttgttgccaa gggcatgagg atctggaaaa
     4741 taaaagggca tattattcaa tgcttctgta actgcgaaat gattggagaa ttcttttcct
     4801 ctatctggag tgatcgtttt gaccgcctca tttggaatac tgcttaaaag ttcaatcatt
     4861 ttctcgctca cataagcggc tttctttctg gggaccttct ttcctgctaa aaggaagcat
     4921 gattttctgt ctgtcaaagt cactaaacaa gtaccaccag ttttgccagc tacaatatcg
     4981 gctttccaat gaccaaaatc acttctatca tctgcggatt ttggacggcc atggatcgaa
     5041 tgagtaaggc aaatctttcc acgagactcg acatgatttt ttgtatgtct tgtttttcct
     5101 cgatggtgca atgaacggat caggccttta ttgccgtgtg agaggggttc tgtatcgaaa
     5161 agatcattgc aaattgcacg ttagatcgtg ataaaactaa ttgaaaagtc catatgttca
     5221 tgcttcaaac gattcgagat ttcctctggc gaccactgaa aatcgacaaa taaatgacga
     5281 acaattgacc aaactcacgg atcatttaga atgtgataac gtccgcattt ttttcttcga
     5341 taagcataat ttttttccgc tttggaaggt gaatagtttt tttcttgttt cttctcagtt
     5401 catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc aattatacta atagaaaatc
     5461 cttcttcgtg aagcagaaag attctttctc gttcgagtat agtaagatga cgatactggc
     5521 tcataattat tttccttcta ggagtgttct cgacaaactt atactagttg gatatcgatt
     5581 atgactcatt tttatgtgtt gcacttaaag tgtaaattca aggtatagaa agaggttccc
     5641 tatgaaaaaa atacctatta caatcttaac tggctatctt ggttcaggaa aaacgatact
     5701 tctaaatcat attctgtacg gtgatcacgg aaaggaaatt gctgtcattg taaatgacat
     5761 aggggaagta aatgttgatg gggtattagt cgacaagaaa ggttttaaaa gaactggaga
     5821 aaaacttgtt tcgatgccga atggatgcat ttgttgtaca ttacgcgaag atttgattgt
     5881 tgaactagat caattagtgc aattttctga cttgaatcaa attgttattg aagcttcagg
     5941 aatcagcaaa ccaattccaa ttattcaagc aattatatta gctaaaaacc ctttagacat
     6001 tgatttgacc caacgattag agattggtgc cattattata gtagtggatg taaaacgttt
     6061 ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag gtaatgtagt
     6121 tgaagaagaa caggatattc gtggcttgtt aattgatcaa ttcgagtgtt gtaacatatt
     6181 acttttaaat aatagtgact tagtgtcaac caagacgatt tacaaaatta acggtctaat
     6241 tgctaagtta cagtccaatg ccaaaataat taggacaatt aaaggtgaag tagacttatc
     6301 gaaaattcta aatacaaatc tttttaatat agatgagact tcctattcag tgggctggtt
     6361 caaagagtta gagcttggct atcaaaatca tattcctgaa acagaagaat atggtatctc
     6421 aagttttatc tatcgaaatc ggttactcct tgacgcacaa aaatttgccc atcttacgga
     6481 taaaaatttt cctggcaata ttacaagagc aaaagggttt ctttggtttg cccaacatag
     6541 tgatttttcg acgatctttg aaacatcggg tagctataaa agtattgaat cattaaatta
     6601 ttgggtagct agtttaccta aaattgagtg tcaaatgatt atacaaaaaa atcccgaatt
     6661 gaaggaagcc tggcatccgt tgtttggtga acgcattaat aaactagttt ttattggtat
     6721 gaagatggat caacaatgta ttaaggaaca acttgagggt tgtttaactg attctcttaa
     6781 tgcagataat ttccgaaaaa tttcaccatt tccatggaaa aagtctaata tgtttagcca
     6841 gatatcgtta atgaaatttt taagaaaagc tggtgtcaag ataggcgatg tcataattat
     6901 tggcattttg attattacct cttttattcc cttatttgta ttctctatag aaaaggagaa
     6961 tgaaacaact gaagatgcta tcaaatatgc catcgttcga attgatggca aggaaatgga
     7021 tcgcttcgat ttggataaga tcaatcataa actcgtgaat tatcatcctg cgaagggaca
     7081 atataatatt gttgagatta aagatggaag aattcgagtt aaagaagata atagtcctga
     7141 tcaaatcgcg gttagaactg gatggatctc aaaacccggt caaacgagta tttgtttacc
     7201 ccataagtta gttattagta ttgagaaaaa agaaagcaca gattactaca tttactaatc
     7261 tggaaataga tgaggaggtt tttaataaga aaaagatatt attattaata atagcagggg
     7321 ctttattaag tgtgttaagc gcttgtggac aagaaaaaga agcggtgaca acaggcacaa
     7381 gtatctttaa cgggacctgc aatggtttag ttaacagtcg gttagattac ttattttggc
     7441 tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata atcgagatac tcacacggct
     7501 agacagctat cttgtctagc cgtgttgttg ttctggcttt agtcagttgg gtagtattct
     7561 tagttttctt aaaatatttt cctcgatata aaaagattcg ttctcggtag ttttgaaagt
     7621 ttctatagcc ataggcaatt cgttttagta ctttgatatg gttattcaaa cattctatag
     7681 ggccgttgga atagggaacg cgcaacgctc gtttgatttc tgtttggtat ttcttgaagg
     7741 tcgtgaacac tggttggtag tgctctggca actccttgta gtcttctttt aggagcgcga
     7801 cgaattcagg aacatcttgt cttctgataa cggaaagaaa atcctggtag acttcgtagc
     7861 ctcgctttaa ggaatcatca taagataata gccgatcgac tatctctgct tctgtcagat
     7921 gatctcgaaa tgacggacgc cagtagcgat gctgaccttt caattcccac gcattctttt
     7981 gaagtagttt ccagtatttc tttaagtgct tcgaccgacg atcccctttt ttaaaggtct
     8041 tcattacttg aattcggtgg ttggtaaaag cccgaccaat gtgttggcta atatgaaagc
     8101 gatcggcgac gattcgggca ttcggaaaac actctttcac caatgtgcga tagggtgtat
     8161 agaaatctga aaccactaga cgcacttgtt cacggacttt tatagggtag cgcaagaaat
     8221 agtcgcgtaa aggattgagt tgccgatttt ccacaatatc aatcagttga tgggtcttgc
     8281 cgtccatcat gacaaagctc atggctcccg agacattttt cactgatttg aattcatcga
     8341 aacacaccac ttctggtaac agctggctca aagaaatggt tttaggccga aggctgcgaa
     8401 gtattcgaat gacagtggtc ggagaaatgt gcttcatacg tgcgatcaaa gacatagaaa
     8461 gaggttcgct taatagctca agaatggctt gtttgactct gcgagcaatg gagcagcgac
     8521 gatcactaac tgaatcttct gctaaaaaag tctttccaca gtgtttacat ttgaagcgct
     8581 gtttgttaat ccgcagaatg gtcttgtagt tactgacatc attcaataag atggatactt
     8641 ttttccagcc ctatttaatg atcgtttgtt cattttgata gtggcgatga aagcattcct
     8701 ccggttgata ggtcaatgtg ccatcaaaaa caaaacatgt ttcgtcattg attcgctctt
     8761 tgcgaaaggc atcttcatga aagataagat ttaggtctag aatatctagt aactctttgg
     8821 taaactctga ataggacatc ttgaaacact cctttgaatg gttttcgtcg actttattct
     8881 aaaggatttt aaggtgtctg tttgcgttta ttagaaaaaa gaaaaagtat tggcaacaga
     8941 aggcgtcacc aacactagat attatagatc ccttattttc ttcgcttcaa attgtcaagg
     9001 agtcatcata aattttgtat ggattctttt gtttgggtag tagtcttcta aataatcttg
     9061 gattaacacc ttcatctcta ttttactttt atatttgttg gagtcgatta attctcattt
     9121 gattgatcga tgaaacgctt caattactgc attgtcaaca ggagtcccag tttggctcac
     9181 tgaatgtgtg atgtggtggt cgttcaacag ctcttgataa ataaaagacc gatgctggct
     9241 acctctatcg gaatgaatta ccgtaggcaa ttctccttaa taaatttgta atggacggac
     9301 gacaatctct tttttcatgt gtgaatctac catatagtaa cgtagaatac gagttgctaa
     9361 gtcaatatag gtggacaaat agaggcgacc gtcattacat ggaatgtaag taatatccat
     9421 cacccaaaca gcatcaattt ggttctgttc aaattatcga ttcaacattt ttttaccatt
     9481 tctttgattg ttttttctct accatgagaa gatttgcgtc gatgaaatcc tcttgtgcat
     9541 aaattcattt cacgcataag aacagctaca acacgtttat tgatttcgat tccttcatcg
     9601 taaagttttt gagtgatccc tggagatccg tatcgatctt tgtgttcgta gaacactcgt
     9661 cttcctcttt tagtcgttta ttttcctgtt caagaatgta gaaatctgct ttatcaggta
     9721 atctttttcc ttgtccagca aaagcatttt tcccgtatcg acgatattct gaaacccatc
     9781 gggaaattgt tggacgagag atattgtatt cttcagcaat tttcttggta gttacttttt
     9841 tctcaaggat atcctgtgaa atctttagtt taaatttacg atcatatgtt tttctcaata
     9901 gagttcattc cttccggtaa cgtaactgat agtcaagaaa agtgtatcat agcccaactt
     9961 agtatcaggg tttgtcaact aaactgtgga agttgataga taagagtttt taacaaccaa
    10021 aattctcttg actatttttg aaatcagaca aattttgatt ggacacatag ttgaataaat
    10081 ctaccgtcgt tacggaaggt aaatcgcatg cttttcaatt ctagaggaga agtcctcgtt
    10141 gaagtaccat agcccagttc tggattcgac ctcctgccca cttcatacgt aaataaagta
    10201 attttaggag tgagactgtg acaaaagtta tttttaagct aaaaacgaat taaaaaacgt
    10261 tgataattaa aatcattcgt tttagattct atgaatcatt atagaacgct gtaaaatcga
    10321 tactttataa ttataaagaa gatagtttca aatttattgt tcgaaatttg aaactatctt
    10381 tttacttatg tcacagactc ccttagttga gttattcttt aatttcgaat ttcgagatag
    10441 ctagatatat tgcaagcata cattcttgat aacaatcttg atcatatttt ccagttctcc
    10501 aggattgctt atgaatcaaa gagctaaatc ttttgagaat tgcctcaaca gcttcttcat
    10561 ttccagcctt tgctaaaatg aaatcttctt ttaaagaatt cataaatcta ccttcttccc
    10621 atggacttat aatctatttc tttgggaagg aggagaacga gcaaagaagc atacaaaatt
    10681 agttattatg cagatattaa accagcattt tttgaatttt tttgtaaagt cgatgtttta
    10741 aatttgtcgc accctgtctt gtaatcccca gaacctttcc aatttcttta tcagttttat
    10801 caaagataaa tttttcgata agaaaaattt gttcaggttc tttcaacaaa ggaaggattt
    10861 ttgctaattt tatatcttca acaaggagag tcacatttaa aacattaatc ggttgtttaa
    10921 attttatagt atctttagta aacaaatatg gtgggagttg atcaagttgt tcatgttttt
    10981 gaatttcttc ttgtttttta taataagtat tagcggtgtt caaaatgacc ttttcaaaga
    11041 atcgaatcaa ctgggcattt acgcgttcgt cgttatctga catattgcac acctcctcca
    11101 ttaagattta aagggaaaag aggcaaaaaa cacgaaaaga agctaatgca attatgctgc
    11161 gctgcgaata acttctataa gctcacatgc attgatagga ctgctgatgt ttattttcaa
    11221 aaatgttttt ttagttgata atggatagtt atctttatac ttagaaaaga aaattcatat
    11281 atcttcttaa atatcaaaca tgtccttatg gctaagaaaa tgtcttgagc tttttacgta
    11341 taataaatct tttctaaaac ctccttttgg ataatattct tattcgtgta tagattgtat
    11401 tttttatttc ttcccccttc ataattaaca actagaaaag agttcgaata gacacgtatt
    11461 tgaacgaaaa tttttattgg attcccaaat tggaatattt agaaacgaaa aattcctatt
    11521 atctgtatag tgcttagata ataacggatc ataataatta ttgtctttga actagatata
    11581 tgtaaatagt attcatcatg atgaaaaatg atgagttctc ggtgataaaa tgtacttttt
    11641 acggtggaat atacatgaaa gcgataagtg gatattttat aatgaagtaa gttagcttat
    11701 tttgacagag gaatagtatt ttagataacg tataaaatac tatttaatga tgtagaaagg
    11761 aaagagagaa acaaaagtgt tttcagataa agaagtctta ttaaagtctc ttcatgtttg
    11821 tttaaagtta cctatcgtgg tttttaattc cgaattcagt attataaaag agtatcgctc
    11881 agatagaact acttattttt tttatgatta taaaaagtta ctaactgata atattaaaaa
    11941 aactaaagat ttccatttct ttactggtga ttttaatgag atgtttcttc tatacatgtt
    12001 taaaaccaga tattacttat ttggtccatt tagagcaaat aa
//
LOCUS       DAYYOA010000081        11669 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000081 DAYYOA010000000
VERSION     DAYYOA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.082"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACHBKA_001987"
     CDS             complement(37..216)
                     /locus_tag="ACHBKA_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445658.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACHBKA_001988"
     CDS             complement(232..432)
                     /locus_tag="ACHBKA_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445659.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACHBKA_001989"
     CDS             complement(597..1304)
                     /locus_tag="ACHBKA_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445660.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACHBKA_001990"
     CDS             complement(1297..1734)
                     /locus_tag="ACHBKA_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445661.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACHBKA_001991"
     CDS             complement(1749..2000)
                     /locus_tag="ACHBKA_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445662.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACHBKA_001992"
     CDS             complement(2000..2206)
                     /locus_tag="ACHBKA_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM9445663.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACHBKA_001993"
     CDS             complement(2643..2903)
                     /locus_tag="ACHBKA_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445664.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACHBKA_001994"
     CDS             3077..3181
                     /locus_tag="ACHBKA_001994"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFM9445665.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHBKA_001995"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHBKA_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9445666.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACHBKA_001996"
     CDS             complement(3810..4709)
                     /locus_tag="ACHBKA_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM9445667.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACHBKA_001997"
     CDS             complement(4722..5315)
                     /locus_tag="ACHBKA_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM9445668.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACHBKA_001998"
     CDS             complement(5391..5540)
                     /locus_tag="ACHBKA_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445669.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACHBKA_001999"
     CDS             complement(5556..7760)
                     /locus_tag="ACHBKA_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM9445670.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACHBKA_002000"
     CDS             complement(7771..9231)
                     /locus_tag="ACHBKA_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM9445671.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACHBKA_002001"
     CDS             complement(9292..11664)
                     /locus_tag="ACHBKA_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM9445672.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1958 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccg
//
LOCUS       DAYYOA010000082        11592 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000082 DAYYOA010000000
VERSION     DAYYOA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.083"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHBKA_002002"
     CDS             6..305
                     /locus_tag="ACHBKA_002002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9445673.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHBKA_002003"
     CDS             378..1739
                     /locus_tag="ACHBKA_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM9445674.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHBKA_002004"
     CDS             1761..2021
                     /locus_tag="ACHBKA_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445675.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHBKA_002005"
     CDS             2035..2787
                     /locus_tag="ACHBKA_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM9445676.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHBKA_002006"
     CDS             complement(2881..3486)
                     /locus_tag="ACHBKA_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9445677.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHBKA_002007"
     CDS             complement(3539..4513)
                     /locus_tag="ACHBKA_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM9445678.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHBKA_002008"
     CDS             complement(4891..5610)
                     /locus_tag="ACHBKA_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9445679.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBKA_002009"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBKA_002009"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9445680.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHBKA_002010"
     CDS             7161..8558
                     /locus_tag="ACHBKA_002010"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9445681.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHBKA_002011"
     CDS             8639..9079
                     /locus_tag="ACHBKA_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9445682.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHBKA_002012"
     CDS             9227..9553
                     /locus_tag="ACHBKA_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9445683.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHBKA_002013"
     CDS             9554..10303
                     /locus_tag="ACHBKA_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM9445684.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHBKA_002014"
     CDS             complement(10383..10838)
                     /locus_tag="ACHBKA_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9445685.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHBKA_002015"
     CDS             complement(11265..11540)
                     /locus_tag="ACHBKA_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445686.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3656 a         2012 c         2355 g         3569 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg tt
//
LOCUS       DAYYOA010000083        11560 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000083 DAYYOA010000000
VERSION     DAYYOA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.084"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..862
                     /locus_tag="ACHBKA_002016"
     CDS             155..862
                     /locus_tag="ACHBKA_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445687.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
     gene            complement(1024..2196)
                     /locus_tag="ACHBKA_002017"
     CDS             complement(1024..2196)
                     /locus_tag="ACHBKA_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9445688.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2402..3217)
                     /locus_tag="ACHBKA_002018"
     CDS             complement(2402..3217)
                     /locus_tag="ACHBKA_002018"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9445689.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3367..3813)
                     /locus_tag="ACHBKA_002019"
     CDS             complement(3367..3813)
                     /locus_tag="ACHBKA_002019"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM9445690.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3810..4325)
                     /locus_tag="ACHBKA_002020"
     CDS             complement(3810..4325)
                     /locus_tag="ACHBKA_002020"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM9445691.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(4398..5312)
                     /locus_tag="ACHBKA_002021"
                     /pseudo
     CDS             complement(4398..5312)
                     /locus_tag="ACHBKA_002021"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(5309..7636)
                     /locus_tag="ACHBKA_002022"
     CDS             complement(5309..7636)
                     /locus_tag="ACHBKA_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM9445692.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            8245..8391
                     /locus_tag="ACHBKA_002023"
                     /pseudo
     CDS             8245..8391
                     /locus_tag="ACHBKA_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(8479..8772)
                     /locus_tag="ACHBKA_002024"
     CDS             complement(8479..8772)
                     /locus_tag="ACHBKA_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM9445693.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            8809..8994
                     /locus_tag="ACHBKA_002025"
     CDS             8809..8994
                     /locus_tag="ACHBKA_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445694.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(9175..10353)
                     /locus_tag="ACHBKA_002026"
     CDS             complement(9175..10353)
                     /locus_tag="ACHBKA_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9445695.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10457..10771)
                     /locus_tag="ACHBKA_002027"
     CDS             complement(10457..10771)
                     /locus_tag="ACHBKA_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445696.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10878..11096
                     /locus_tag="ACHBKA_002028"
     CDS             10878..11096
                     /locus_tag="ACHBKA_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445697.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(11016..11486)
                     /locus_tag="ACHBKA_002029"
     CDS             complement(11016..11486)
                     /locus_tag="ACHBKA_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9445698.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
BASE COUNT         3347 a         2262 c         2018 g         3933 t
ORIGIN      
        1 aacagaacta tagcaaaaaa tcccgtgtat caacacggga ttagattaag tttcctcaat
       61 cactattcat aacacaatta ttataacaag taagtaaaca attgaaaagg acatttattg
      121 aaaacggttg ttttttactt aaggaggaaa tttagtgaaa aaattagttg cgttgttttt
      181 ggtcgcagga agtttgttga gtgggtgtgg tgctactacg tctactactc aaagcacaag
      241 ttcagctcag gcttccagca gcagtaccac gatctccgaa agtacggcag attcatcgaa
      301 agaacaaatt gaaaagaaaa aagaagaaga gaaacaaaaa gcggaaaaag ctgcactaga
      361 aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga
      421 aaactatgca actgcttcag cagctatcca agcgctacct gaaaacaaac aagagttgac
      481 aagccgtctt gctgcaattg attccacaat caaagcaaaa gaagcagaag aagaacggaa
      541 aaagcaagaa gaaattgcac gacaacaagc agaacaagcg caacaagaag caatcgcaca
      601 gcaacaagca gaacaggcga ggcaagaagc aatcgcacag caacaagcag aacaggcgcg
      661 acagcaagca gcagctgaac aggcttctca gcaggcacaa gccgcagctc ctgagcaaaa
      721 taatgaacaa actgtctatg tcacaccgac cggttcaaaa taccatacac ataaatgtgg
      781 aaatggtacg tatacccctg ccactttaag cgaagctcaa gctcgtggtt taaccccttg
      841 ctccaaatgt tatggaaact aattcaaaag ttatactata ataataaaat tcttctgaaa
      901 cataaaaaaa tagaagctgg cgttccagat agtaaaccaa aaatagcagt ttacacgtcc
      961 atttcgtcag cttctctctt ttttcattta gtctgttccc tctgcttttt tggacatctt
     1021 cattcattct ttctcaatca aatagtacac ctctttttct tttcgcagtt tatatcttcc
     1081 ttttttcaat ttcattgaaa actgtttcga atgtttatgt tttacatgga ttttaagcag
     1141 gagctcctca tgttgcaagc gatagttcct gccttttaaa tcagaaaatc tcactagtcc
     1201 gtattcatca gaagctttca caaatggaac tttttctcta gtaatatagt gtttgccgtt
     1261 gagcgttttt acagaaaaga ccattccttc gattggtcgt ccgtttgtgt tctttacata
     1321 aaaagataaa ggatagtttc gcttagcgat ggccaaagaa tctaaataag cgatgagtag
     1381 ccaaactaag ccgattgatc caagtagtat accgataaac caaataaaaa atttccattt
     1441 tttccattga tcaattcctt ggatcgcttt ttttgcctct tttgcttgat aaggaatacg
     1501 atgccccgtc acaagcagtc gatgtgtatt gaccatataa ggagtgcagg taagcaaagt
     1561 cacgagatct ctgccttttt ggatacccaa agaatctact tcgtcaggta agaccacttg
     1621 gattttttct acttgatacg ccaatgtctt tccattgatc tggatgaaaa actggtctcc
     1681 ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct gggagtccac gatgcccaga
     1741 aagaacggca tgggtgtttt tcccgccagt cggataagaa cttccttcca ataatgacgt
     1801 tcctttttgc aaaaatattt ccgttgtctg atcgaatata ggtaagctga cattgatttt
     1861 tgggatttcg atgactccaa gcatatgttc cataaaaaag gcattactag gtaaatcttt
     1921 taatgctttt gcatcaactg cttggttgaa actgcctatc cctggtgata cgttttgctc
     1981 tgccaactgt tggtttttct ttgtcatttt ttcctgctgt ttttttattt cctctgaatt
     2041 cttattcgat gcttgctttt gataatggat gatcatttgc tgggcaagaa aatcattcag
     2101 tgaatcttct acaaaaggat aagaaaagat cccgatccca acgactaaaa tgattgccat
     2161 gaagatgtcc agtattctta ttctgctttt attcatcatg tccacctatt ttctaatatg
     2221 tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa
     2281 atagttcctc ctattttgcc gtttgttgag cgaataccaa gagaccgacc acttgtcaca
     2341 acctttctcc ccatttgaat cactatcgtt tatttatcgt atgatttttg tttccaatag
     2401 tttaagcttc tgtttcttga tggcgttttt tgaaatagat cactgctcct ccaactaaag
     2461 cagtacctac caagatgaat acgatgatcc ccataccacc agttgaaggc aagattcctt
     2521 taggttggtt atcgatcgtt gtttcatcag aagttgttgg atcatcatct actgtaaatt
     2581 cagtgtacgc agcttcattc ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa
     2641 ggcagtatgc cccttgtttc agcccttcga ttgaaaattt cccttctgaa tcagaaatca
     2701 atttcacttc ttcccagtca gaacctgtga cttcatctga ccaagcatat gggtattctc
     2761 ctgaggcaag gtttgctatt tttttcatta atttaacagt tggatctttt gctcttagaa
     2821 tagaaaattc tgcatcggct aatttttctt gcgtgttcac atcttgtttg atgaagtgga
     2881 aatcaccgta gttttggaca ttttctacag taatcgtgtt atccgtagtt gtattttccc
     2941 gattgactac accgtttgga ttcccaagat tggcttcatc tgcgccatgg atgataccgg
     3001 ttgtttgttg caattttccg ttttttacta taaaagtagt ttctttttgt ggattggtta
     3061 attgataacc atctggtgct tttgtttcaa ctaaagtata attgccgtct attaatccat
     3121 tgacactgat ggatccttca gtatcagttg tgaatgtcaa cgctgactca gcaggatcat
     3181 agagagcgac accagtgttt tcatcaaaac cggtcacgaa ttgattctct gaatttttga
     3241 tctggaattg cgcccctgct aacttatcta cccctgaaaa gttcccagct ttctggattt
     3301 tgatattgct ttctttgatg acatttttag catataggtg aatcgtgtct aaaggtttgt
     3361 cagggtctag ggtaagctta acacgatatt tggcgacgtt tcgacaccgg gctttggtgt
     3421 ttctacaaag acgtatacag catctttccc atcagattta cctggaagag tcaggttagc
     3481 taatccttca ccagaagtcg ttacacttgc gaagggtgta gcgtctgctg gaactgctgt
     3541 gtctttactt ggatagtacg tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa
     3601 cggttcgcta ttcggaaatg tcaattccgt tccgttgttt atttgatttt ctggtgctga
     3661 agtgaatttc aatttatgca aattaaccgt aacattttct ggtgtaggtg atacggctgc
     3721 agaaacctct gcagttccta ggccgagaac gaaaggaagc agcatgatag cagttcctaa
     3781 aaacgtccat atttttgttt tgatgttttt cattttattt ccctcctatt gtttcctttt
     3841 tgttgttttt tataaaacca agctgtccct gttgaaagga ctagcaataa ccctaaaatt
     3901 gagaaacctc caagcatctc ttcgcttgtt tttggaaagc ttcctccatt tttttcattt
     3961 gatggaccat ttgtctgcat ctgaactggc gtttttccat tattcgtcgg atttgctttt
     4021 ggttcgttga tgatttccaa tggtatagga tgaagcggat cataactgcc tttttcaact
     4081 tcaaagagaa tggttgattg actcaatata tatcctttag gcggtttaat ctccactagt
     4141 tcgtaacttc caaaagacaa tccatggaca gaaaatgttc catctttact ggaagtaaaa
     4201 atcgttgctc ttgaatcatc tttggctacc caatgattta tcccgttttc ttgcgctaag
     4261 tattggtcat tttttttgac tagaaattga gcatctgcta gtttttgttt cgtttgccga
     4321 tccactttga caaattgttt gcctcctgtt tggatggcaa gatgtgtttc tactgtttga
     4381 ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt tttcactgtt ttctttcaat
     4441 gtcatttggt aacggatcgt taattttttt ccagcaaacg aactaagatc ttttccagaa
     4501 tctgataaga caaaaccatg ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca
     4561 ccaatcaaaa aatcgatcgt ttctggttct agccataact gaggatcagc tgtatctttg
     4621 atcgtatagg ttcctatctc gttgatgttc tcaggaattg ttacggtgat gagataaggg
     4681 actttatccc catctgcaaa atctgtctgc ttattctcta ctgtttttgt taatgtgagt
     4741 gcagcaggta ttttttgttc gctctttgga tacaagtgga tggtcgtcaa tttatgatta
     4801 gatgaatctg tcacaggaag cacaacgaca aatggtgctg atttttcttt tacttaatct
     4861 gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat
     4921 gcactaaaag aagcaatgcc ctcttcctcg tctactgttt ttgtcacatt ttctgccagt
     4981 attttttcac tatcactttt ttgtgctagt tttctttgtg cgtcttccac actacttcct
     5041 tctgaccgca gcttgtaaaa ttcatccgag acatcatata tagcgaaagt aacgccgttc
     5101 aaccctttta ctccttgaaa gttatccggg atatttattc cgtcgttttg actgatactt
     5161 ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa atgtaatagg tgattctcta
     5221 gctgacacag tcggagaacc tataaaaaag accagtagga taaaaaagag aacagcaaat
     5281 atatccacac gtacattttt tgtttttttc atcgctgttc acctgctttt ttcttacgat
     5341 ggataaaata aaagaagcca gaaaaaataa agatcagtcc taaaaagaca aagggcagta
     5401 gacctatccc acctgtcgaa ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat
     5461 ctgttgcgat aatcgtataa ttattgttta cacttccttc gcctaaacta atcgttacta
     5521 tttgtgatcc aatatctgtc cggctgttaa ctgttagggt ataatccaat gattcgccta
     5581 agacaacacc ggtaacgtgg agatttccaa ccgaaatcga gtgactttgg gcatcataat
     5641 taaaggcagc gtcatcgaca gtaatcactg cgttagcttt atagatggta taagttcctg
     5701 atgtgttggc tatatcagta aatgacaact tgggatttgc tgctttgact ttgtctattc
     5761 cgctattcgt tagtttaatt gtatagctgc cagcattaac catatttttt gctgattgtg
     5821 catctgctgt gtaatccata ctgctaagtg ccacttgtac tggagtaagc gaactagtga
     5881 caccctctgc tagcttgaca tggattgacg ggacacctgc ttttccatca tatgtcttac
     5941 tgccactgag ctctaccgca ccgctgattt gcttaatcgt atagacatca gatgtctgca
     6001 cgatagtaaa taaatagttt gttctggcag ctagtttagc tagtccgctt tttgtcaatt
     6061 gccaagacgt ataggttcca atttcagtag gcgcagttac ttcaactccc ttttcattca
     6121 tcgctttgaa atcagttgga ttccatgtga tttgatcaat actgagatca ttgtttaaaa
     6181 gggatgttga ttctgtttta tcattaaagg catatttaac gatcggtaca acagaaagac
     6241 catcatagta cttactgcca tttgcattga ttgatactgt tgcatttgtc ttataatata
     6301 ggatgacttc agaaatctgt tttccatttg cgtcctttac gattacattt ccatttgtat
     6361 ctaaactagc taaaacactc attgctgatt ggttctttgt ttctatcgct gaaacagctg
     6421 taaaggtata tccgttgata gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt
     6481 aagtaagatt cattatttta gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa
     6541 tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc gacgttactt tctagggctg
     6601 tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac tgctgttgga gtgataacca
     6661 ctttgtcacc cacatggatt ccagataatg tagtcccgta cgttccgatt tttgctgctc
     6721 caccattgac tgtataagaa aaagttcctg tccgattcgc attgacctga accgttgctg
     6781 cttgataggt tggaaaattt gaaacatttc ccttaccatc aacgatctgc ccatttttga
     6841 tcgagtaccc cgtgaatgga atatcatttg ctaactgtat accgattgaa aaagtactca
     6901 tacctcctcc ggcaggaagc cctccccatg ttgagggtgt aaaagaataa cttttatccg
     6961 cattgggtgt aactgaatcg atcgtatttc cagcggtagc cacccccata tagatacttt
     7021 ttgggaatgt gatagtagta ccatttactg tggagccaat aaacagatta tggatatctg
     7081 gacggttatc aataaaagcg attgctttgg ttacgccttg taacgcagtt ttatccgcac
     7141 tcatcgatcc gttaaccgca acaatgactc catctttggc atatatcact gtatatccat
     7201 ctggtatggt atgtccgttg aaattcgcag tggccgcaac agtactaaat gtctggcttc
     7261 tacctgtttc tgaataagct ttctcccctg ctgcttttgc tgctgtgatt tctttttctg
     7321 tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat
     7381 tggtgctttc tatggactgt atttcagaat agggtggact atttgtctcc tctgtcgttt
     7441 ggttagcaaa atttgacgtt tctgtataaa cgtttacata aatacttgat aagctcccta
     7501 agaaagtaaa aatcaagatg atcgaaatca taatgggatg taagtatttt cttttagata
     7561 cagctttgag tctagaaaca aaatgttttt gacatatacg ttggatttta ttttgagttt
     7621 tcttaatcat tttcatccct cctctcaata aaaaatcatc aaataatcct ttacacgtta
     7681 ttctaaattt aatttttatt attttattga tgtatagaaa gtttggggct ttttttctct
     7741 ttttattcta aggttatttt agtcttgtcc tttaatacaa agattttatt tttaaataaa
     7801 aaatactctt tttttacgat tattgaaaaa aaaagataaa acttgattaa ttgaagtatt
     7861 gcttaaattt ggaagctatg agggaagaat aaaagagtta ggacgggatt atctactatt
     7921 tttctttgtt ttaaatattt gttatctttg aacataaaag agaaatgtgt cgactgcagt
     7981 gttgtgtccc tcatcacgaa taaacggtgt tcaattagct gatcttgtat tcacttaaaa
     8041 aatggtaaag tacgagttgc tattccccat tttcaatttt ctaacgctca ggacaaaata
     8101 ctgttttcac aacctcattg gaataaaagc atatatattg tttcaagaac gaaatcttgt
     8161 tatcttttta aagggcatct gtccaaaatc taattttagg aggtacgttt tggtatttat
     8221 tcgtacaaat aaaagagaat tatctttatt catgtaacgc atccaattga ttttttcatg
     8281 tacgctcctg gaacgatttt actgacgatt gcttatttaa ctgcgaatcg ctcattagct
     8341 tttatcatag ctatccatat tttgaataac gttcttggct ttgtattata gatacagatg
     8401 acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc atacttgaac ttttttcaat
     8461 gagtataaat taaaacttct aattttcaat tcccatcatc tgatatgccc gaccgtctga
     8521 acttcttttg aaaaagccaa atgtgattag ataacgtctt tgttcgacat aatcgccact
     8581 agaaaatacg ttcagcagta tcgaattaac ctctgtttct gtataagtca ctccatggtc
     8641 aaagcgtttc aagacttctt tcaataggat gtatttcttt tttgttgaac ttggtacttg
     8701 ttttactttt ccatcattca tgaatgtgtc taaaactttc atcttttctt gctccgtata
     8761 agtataaacc atcttctttc tccttttgat tctaagaata ctccatttat gttagaatat
     8821 tctggatttt ttattaaagc ggttactctt aagaaagata ttatcatagt gctctttttt
     8881 ttactactac ttataggtag atttattcta atccattatt ctcccctacc aataattaaa
     8941 gaaaaaactg gatcagggtg ttcaaattac atgctaagaa aaaagaaccc ttgaatacca
     9001 agggttcttt ctcagtctga taacaatgat taacgacgga tttctttgat acttgtgaaa
     9061 agtcgttaaa gtattgatat aaaaggattt ttattacctc tgactacata ttgactaatt
     9121 tgttaaaaaa tcgtttttca agaatcctgt aataccagtg ctttcaccgt ttttttaact
     9181 ctctaaagaa ctaaaagcaa tcagaggttt taccttttca tttgacgtta agtggcgata
     9241 aattttcgaa gttgtttggt cagtatggcc taagcgatct ttcaagactt cccagctaca
     9301 gtgttccgct actaagcttg ccattgtatg acgagctaaa tgagtatgaa atggcttatt
     9361 aattcctgag cgattcacgc accgtttgac gtgatcattt acatactctc gacgataagg
     9421 aacaccacgt ttactagaat caagatacac aaacaaatat gtttcttcta taataaacct
     9481 gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt tgtagctttt ccataactag
     9541 aggagttact agaactcgtc tagcagaact aactgtctta gtagtttcaa ttcgaaaatc
     9601 cttactctta cctttaactg ccgtttgctt atagaacaga atttcatttc ttttgaaatc
     9661 tatatctcca ggctgtagag caagcgcctc gctgactcgg cagccagtgt aaaatagaac
     9721 atggaaaagt acagacatcg gtaattcctc atgaacaatg ccataattca aaaaagttct
     9781 caattcatcg acagtaagaa acttgtcctc aatattattt atggcttctt ttaaatcagt
     9841 taccgttaac tgatattttg gcattctcac agacttcatt ggattttcac ttatcaaatt
     9901 ctttctttga caatatttaa aaatcatatt tagaacttga ataagtgatt gtaaactatt
     9961 tttggcatag ccaacatttg ttccattaac gccacattcc tttaaggtaa aaagcaattc
    10021 ttgaaggcac tctggtgtca aatcagacaa ctttttatca ccaatccatc gaacaataat
    10081 ttttaattgt tcctctcgct tatcataggt tatttccttt tgttcattta acgaacggta
    10141 atattggaac cattgctcac ctacaacact aaacttgtca ttgcttgcaa caattttttt
    10201 cttaagcctt tgttgtttct ccatttccag ataagcgagt tgcgcctcac gttttgaatt
    10261 aaagccacgt cttgttgttc gatatgcttg aaagtaccat gtgatcctac cagtagaact
    10321 gatttgcttc ttgaatcgat ctgttttttc catttattga ttcctcctat tttgaaggaa
    10381 cccaacaagc agaaaccctt tggaaatctt tctatctatg tcatcattgt accgcatctt
    10441 agaaagaaaa tgtaacttat aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg
    10501 aatcactctt tctcatccat tctaaaacat catcacgaaa atagtgtcct cgaatcccgt
    10561 accaacaggg aggaaactca ccaagtttta ctaaacggtt cagtgtacta tcacttatct
    10621 gaaatagtcg ctttaaatca gtatttgtta ataccagagg taaatgactg actgcaagca
    10681 atttttccgt gtaatcttcc agtttttttt gtaaaattga atagaactct atttccagag
    10741 aattaacatt tgttttaact tccaactcca tcacccttct ctctcaaaat caaaacgacc
    10801 accacggtac cccttaaaat tgtaaaaaaa cactccagta ctccatcaaa acggactaga
    10861 aaagaggaat ttttaatatg aaacaaggaa ttgcagacat aaaaataatt aaagaaattc
    10921 ttgaaaaatc tacggctaat gcaatagcat tcggaacagg aatcaacttg agtacagtaa
    10981 aaaaacttaa aagtggagag agagctgaag aaaaattaaa tcttgccgat gctattaaaa
    11041 tcacagaatt tggcatgaaa aatatgccaa ccaaaatcga aatatggaaa tcctagctct
    11101 tccaaagcca tttgtcgttg gtaccaaaca ctgccgagac ttttcccaat aatatcgcca
    11161 atttcacgat cattaagacc gatgaagaag gacataaaaa tgatttcttg tctttgacta
    11221 ggtaatgctg caatcgcatt tgccaactcc tcattggaaa taaagatttt cataccaagg
    11281 acaataaaag taccactact attcaaattt ggttccaaat aatagaactc tgtacctaac
    11341 cctttttgtt ccaatatttc cattgatatt tgccgttggc gaaatctagc gtacttcttt
    11401 tgaatattta acgcctcatt gcagactact ttcttacaaa aactgtcaaa cgtatgttga
    11461 atgtgggttt cctctgagtc gcccataata ttctccccct caaaaaattc attcaactcg
    11521 ttttacttct atccctttcc attattacta caatctagga
//
LOCUS       DAYYOA010000084        11432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000084 DAYYOA010000000
VERSION     DAYYOA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.085"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..579)
                     /locus_tag="ACHBKA_002030"
     CDS             complement(34..579)
                     /locus_tag="ACHBKA_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9445699.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(591..1283)
                     /locus_tag="ACHBKA_002031"
     CDS             complement(591..1283)
                     /locus_tag="ACHBKA_002031"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM9445700.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1302..1586)
                     /gene="macP"
                     /locus_tag="ACHBKA_002032"
     CDS             complement(1302..1586)
                     /gene="macP"
                     /locus_tag="ACHBKA_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM9445701.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1599..2162)
                     /locus_tag="ACHBKA_002033"
     CDS             complement(1599..2162)
                     /locus_tag="ACHBKA_002033"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9445702.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2390..3052
                     /locus_tag="ACHBKA_002034"
     CDS             2390..3052
                     /locus_tag="ACHBKA_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM9445703.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3176..4372
                     /locus_tag="ACHBKA_002035"
     CDS             3176..4372
                     /locus_tag="ACHBKA_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM9445704.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4473..5849)
                     /locus_tag="ACHBKA_002036"
     CDS             complement(4473..5849)
                     /locus_tag="ACHBKA_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9445705.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5886..6752)
                     /locus_tag="ACHBKA_002037"
     CDS             complement(5886..6752)
                     /locus_tag="ACHBKA_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9445706.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6841..7866)
                     /locus_tag="ACHBKA_002038"
     CDS             complement(6841..7866)
                     /locus_tag="ACHBKA_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9445707.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7884..8558)
                     /locus_tag="ACHBKA_002039"
     CDS             complement(7884..8558)
                     /locus_tag="ACHBKA_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9445708.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8742..9635)
                     /locus_tag="ACHBKA_002040"
     CDS             complement(8742..9635)
                     /locus_tag="ACHBKA_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9445709.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9709..11406)
                     /locus_tag="ACHBKA_002041"
     CDS             complement(9709..11406)
                     /locus_tag="ACHBKA_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9445710.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3386 a         2515 c         1964 g         3567 t
ORIGIN      
        1 gtgcttttta ttttatcaaa tcactttgtt tttttataaa atttctgcgc ctagtgtgga
       61 ctcaaaatgt ctcaatgccc attcatgtcc aacgggatcg aaacttgcca cggcatcttt
      121 gaaaatatgg agtttgtatc ctagattata cgcatcgact gctgtatgca acacacagat
      181 atctgtacat acgccggtca aataaatatc tgtgatctgg cgttctcgca aacggatgtc
      241 tagatctgtt ccactaaaag cggaataatg ccgtttgtct atccaataaa cattttcctc
      301 ttgctcatag tcttggtata aactttttaa agagccgtac aattcccgtc cacccgttcc
      361 tatcacatta tgcggaggaa ataagcggtt ctccggatga aaggaatctt ttggatcatg
      421 cgcatcaatg gcaaaaacga caaaatcctt ttgatcaata aacttctttg tatgagcagt
      481 cagctttttt tcaattactt gtcctgctgt cccagtagtc agctttccat cctctgcaac
      541 aaaatcatat gtgtagtcaa tcgagatcaa tgcgttcatc ttagtcctcc ttatacgaga
      601 tgtttgacaa attcgatcac catttctgct gatcgtttgc ctgcgtcttc gataaattcg
      661 tcaaagcttt gtgtcgccga gtgatctgct gtgtcgctca ttgcacgtac gatcaaaaat
      721 gggatattga actgacgagc tgtttgtcca acagcggctc cttccatttc gcaagctaat
      781 gcttcaggga aattcgttaa gatttctttg attttatctg gcgagtcgac aaatgtatca
      841 cctgtaacga tcaatccttc ttttgcgttc aaattcgttg cttttgctgc tttaaccatt
      901 tcactgcgca aatatgtgct tgcttcataa tatagaggca ttcctggtaa ttgtcctggt
      961 ttgtagccaa atcctgttgc atctgcatca aaataagcta ctttgtctga tataacgatg
     1021 tcacctactt gtaagccttc accgattccg ccagctgaac ctgtattgat caccatgttc
     1081 acgccgtatt gttggatcaa cagacttgtg gtaatagatg ctaagacttt tcctataccg
     1141 gaacgtacga cgatcacttc gtggttacct aatgaacctg aaataaataa agcgcctgcg
     1201 cgttcccatg ataatggttc gcttaggttc tctctcaaaa tttttacttc ttcttccatt
     1261 gcgccgataa taccgatttt catcttttta attccttctt tctatataaa tgcgattgct
     1321 aaaaaaacca cacataacat taggataaca ataataagag atttcatcaa aaaagagttc
     1381 catttggtcg tcttttcccg ttcgctgatc cttgttttcg tgattggttt attctttttg
     1441 ctttctttac gataaaagct ggcaatcttt ttttcttctt gctgataagc tgtttctgct
     1501 tttcgctgtt tttttaatga ctcgcttgct tgtgcttgtt gtcgcttgcg aagttcgctt
     1561 cgcgtaataa gcggtccttt ggccataagc cgatccctct actttccttt ggtcaatagt
     1621 tcccagtatt gaatcgcata aatcgtcttt gcatcacaaa tcaactgatc tttcatcgct
     1681 tgttgcgcct cttcaagagt tagatgatac aactccagta cttcgtcttc atcttgtgct
     1741 aaaggattct cgaccttctc taccccttgt gcatgataaa tatgaaggac ttcatttgca
     1801 aaacctggtg aaagatacat cgaagtaaga tggctcagcg acttcgcacg atagccagtt
     1861 tcttcttcta attctctagc tgctgtcatt tcgggatttt gtccttctcc tggatcgatt
     1921 tttccagctg gaatctccaa aatcactttt tctaaaggtt ttcgaaattg tttgacaaga
     1981 agcagacgat cttgttcgtc aaaagcaatg atcccgacac caccaggatg aaataccagt
     2041 tctctcttcg aagttcctcc atctggcaaa cgaacttcgt ctacagcaac atcaataatt
     2101 tttccgctat agatttcttt gcgactgatt gttttttctt caaattgctc attgttcagc
     2161 atatcttctc ctgctttctt tgtaatcata actgcttttt tttcattttt caatttttct
     2221 caaaaactcc ttattagttt accatagata aaccttagat tttctctaaa tatagaaaat
     2281 ccttctttag tccgatactc tttctagtag tccgtatgct aaaataagta tgtaatcgaa
     2341 taacgaaaag aattcaatac tcaacgataa atgacatgga gggtttatta tgataaaacg
     2401 aaattggccg tggcttctag cattggtctt gctatttctc tttgtccgaa acaatagttt
     2461 ttcttttgtt ctaggacttt tattgatagg tgtagtttta gttttgtggg gactctttgg
     2521 ttcgcgcagg aaaaagggaa aacaagaaga aattcctgca ctgactgacg aacttgaaac
     2581 tcactatgaa aaaagcggta tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa
     2641 tcaaacaaaa aatgagatca atcaattgca aatgaatatg cagcagacag ccaaacttaa
     2701 agcaatcgat ctccgccatg atccagtcaa agcagcgaaa gctttattca aagaattggt
     2761 gaaagaacca aaccgactgc atgaagcaag tcagtttctt tatacgcatc tgccgaatct
     2821 agttgatttg acaaataagt ataatgagat caatgaccat gagatcaaaa acaaacagac
     2881 ctatgaaaaa ctagaagaaa gcgcgcagat catcgaccag ctgtcagctt taatcgcaca
     2941 agattatcaa aaattcgttg ctgaggatct agatgatatc gacgtagaga tatctgttgc
     3001 gaaacagagc ttaaagcgtg ataataaata tgaaccagaa caaaaggatt aactcttttg
     3061 ttcataaaag aagactcgga acattcagat gatgtctggc tgtttcgagt actttttcta
     3121 aagtagagac attcatgtaa gaatcgagaa cagattcaac aaggaggaat tttttatgga
     3181 tcataaaccg actgaaacaa aagatgttac accagttaac gatacacttg aagatttatt
     3241 gaacaacccg ttttcaactc caacagatac gctgaccact tcacaacaag cggagattga
     3301 tcagctccaa gagcaacaga cagctgctcg tttaattgac aaactaccgg cggaacgcca
     3361 agaacaagct aaacagctag cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag
     3421 ctacggatcg gctgcccaag caaaattaag tgaattctca caatcgatgt tgaatcatgt
     3481 gcaagcacaa gatattggtc ctgtaggaga ttccttaact gaactgatgt atcgtctcca
     3541 agaagccaat cctgacgaac tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg
     3601 gaaggtcaaa caatcgatct atgaagtgac agcaaaatat caaaagattg gtgcacaaat
     3661 tgacaagatt tcagtgaaat tagataaaga aaaagacggt ctgctaaaag ataatctgat
     3721 gttagaacag ctttaccaga aaaacaaaga ctactttgat gcactgaata tttatatcgc
     3781 tgctggagaa ttgaaaatgg aagaacttca aacaacgatc attccagaag cgatgaaaag
     3841 agcagaagaa actggcgatc agatggatgt tcagattgcc aatgattaca cgcaattcct
     3901 agatcgtctg gataaacgta cgcatgatct tcgcttagct agacagatca cgatccagca
     3961 agcaccacaa atccgtttga tccaaaacac aaatcaggcc ttggctgaaa agatccaagc
     4021 atctatcgct acagccattc cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct
     4081 tcgtcaaaaa gatgcagtaa ctgctcaacg ccaagtctct gaaacaacaa acgacttatt
     4141 gaagaaaaat tcagaaatgc tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg
     4201 tggaatcgtg gatatcgaga cccttcaaac cactcaaaat gatttgatcg aaacaatcca
     4261 agagacattg cgtatccaaa aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct
     4321 tggacacatg gaagaagatc tgaaacaaaa actgttggat ttaacccaat agatcacctt
     4381 tcgcttgtta ctaaaacaaa cgcccaacaa cagtttatta caactgttga tgggcgtttt
     4441 tcttatcttt cttctaactt ctccattgga gtctatttct cattcatctt gctgtgtcca
     4501 tagccatagc caaaataaat gatcagtcca ataaccatta cgataccgaa cacgatccat
     4561 gtcacagttt gcagacgaag catcaataca atacaagtca agatggagac gattggcaat
     4621 agcggaacga atggcacttt gaattctcca gcttttggtt tgccaaacat ttttctcaac
     4681 ttcagtaagc ctagactaac aagcatcaaa taggcgagcg ccatgatatt cgttagttcc
     4741 gctaatacat tcaatgggaa aaatccggca aagaatacag ctgccacacc agcggcataa
     4801 gtcgcttttt tcggtgtacg atgcttttga tcgatttctg tcataaactt aggaagtaag
     4861 ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat acatcatcga gatcgttacc
     4921 gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc ccactgttcc atgtcctaca
     4981 tacctcattg caaaagccac tgggtcattg actcctagtt cagtatacgg aacgattcct
     5041 gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa tcagtatcga accaatgatt
     5101 cctcttggca catctttttg cgggtttttg acttcctcgg ctgccattgc aacggcatca
     5161 aatcccaaga aagcaaaaaa cacaagagct gcaccatcta gcacaccgga aaaaccaaaa
     5221 ggcatgaatg gctgccagtt atcaggttta acgaaaaata ttcctacaat gataaacaac
     5281 gcgatgatac caaatttgac atataccatg gcattattca aacgtaatgc tttcttggct
     5341 tcaagcgaca cgatgtaagt gacgaatatc actacgagca ccgcaatcaa gtcaatgtat
     5401 gtaccatttt ccggattgta cccgccagtt aatgcttgtg gaagttcggc ccctaaactt
     5461 ttcaaaaaac cttgcatata gccagaccat ccggaagcaa cagaggaaac cgctagtaaa
     5521 aattccccga tcaacaacca ccctgtcagc caggcagcaa attcaccaaa tacaacatac
     5581 agataggcat atggcccacc aatcactggc actcgcgaag caaattctgc gaaacttagt
     5641 ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa gtgacagtgc tggaccagca
     5701 ttttgattgg ccgcaacccc tgtcaccaca aatattcccg taccgatgat cgcaccaatt
     5761 ccaagcatga tcaaatcaga cgtttttagt tcttttttca tctcactcgt atgattgaga
     5821 ttgatctctt tcttacgaaa caaatccatt gaagttcctc cccattttag gttggaaaac
     5881 agcgattaaa ctgttttcat ataccctaaa aggataccac ctaaaatgac taaaaggcaa
     5941 ccaacaataa cataaatgag ctcttttttc gtttttcttt cgccaaggat gaaaatccca
     6001 cctaaagtag agataatgat ccccatttgt gaaagtgaga agccaacagc taaaccaagt
     6061 gatttgactg ttagaagcat gcagatgttt cctaagcccc ataataatcc acagatcatg
     6121 tttttccaca caaactgatc gactttaact ttacggaaag caaagaagct tgcgcctaag
     6181 atcattccga tactttgagg taagataatt gacatcgcat ccaaattcgc ccagttgacg
     6241 atgatggtat atacaccata accaatcgta gagaagatca atgctctaaa acccttacca
     6301 aaatcaagca ttttattatc tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa
     6361 tacgcaccgc ttacaagaag agctaaagca ataataccgt atacataatc tttcgtttgt
     6421 gtccattcat ggaagaaaac ggctcccgca atcgtattca aaattaattg aaaaccagta
     6481 gacaatggta gccctacaga aacacccatg tacttcattc catggaactg tccattttgt
     6541 ccaacactcc aagctaagcc ggataaaaat ccgatcaaga aaatccaagg ggtgattaca
     6601 ggactaacaa cgaaaaagac aactaaagaa aacagaaatg caccgatcgt cattcctagt
     6661 gtttgttgat tggcatcgcc accaatttta ccacttacta aaccaatact accccatgcc
     6721 aacataggga ctaatgctat taaaatttcc ataaccaatc tccttttatt tcacatgata
     6781 agaaacttat cagcgaaaac gttaacaaca caagcacttt ttttcgtttc taacgatttt
     6841 ctaacgtttt tcattataaa aatttaagat tgggaaaaat aaagtcattg ttgtcccttt
     6901 tccttcagtt gattcagccg tcaaatggat acctaatttt tcggccagat tttttgctaa
     6961 ataaagtcct aatcctgttg aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc
     7021 cttatcaaag atccgtttca agtcttccgc tgggataccg atccctgaat ctttaactga
     7081 aaggctgact ccttgcgctt ccttttctat gctcacaatg atgtttccgt gatccggcgt
     7141 atatttgatg gcattactaa gcaattggtt gaagataaaa gcgatccatt tagcatcggt
     7201 caaaacagta tgatcctcac caacgatctc gtagcgaata tttttttgga taaaataatt
     7261 tgcctgactg cgtatcacag gctgaatgat tccttttagt ccatattctt ggatgaggta
     7321 gtcccgcgag aaactgtcga gtcttgcata atacaaaact tgttctacgt attcatcgat
     7381 ttttgataat tcattttcta gtaatgtata cttttgatcg tcaatatcga actcgatcga
     7441 acgaactagc aatttagaag cggaaagtgg cactttgatt tcatggaccc atgagtcaat
     7501 ataatctttt tggtcttgtt gattactgat tgctgattgc attacttctt ggtgttcacg
     7561 gatcagctga ttgatatatt cttctaccag tttttcttct tctgtcctcc caccactcaa
     7621 ataattttgc aaaggggaag ttttttgtat gtcggctaat tttccccacc atcttctctt
     7681 gctcaagtaa ccacccagca gataaaaaat cagaaagacg ccctcaatca aagctaaata
     7741 agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa
     7801 tgcaataaaa aacagccatc ccaagatcaa tgtccactga tctttcaaat atttaaaaaa
     7861 attcatctta accgctcatc ctttcaggga acgatatatc cctgtcctac ttttgtttcg
     7921 atatatcctt ggatacctgc ctgttcgatt tttcggcgta accgattgat gttgactgtc
     7981 aatgtattgt catctacaaa tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga
     8041 cttaagatct ttccattgtg cttcatcaaa ataaataata gacgatattc atttttgctc
     8101 aaatcaatga tctctccgtt gatttccata cttccgttgt caacattcaa cgtaatgcca
     8161 ttgtggctca tcatttcact agataattct gtgtagttgt aagaacggcg aagtaatgca
     8221 ttgatttttg caatcagtac atcgatttgg aaatgttttg tcacaaaatc atctgccccc
     8281 atgttcatag ccatgatcat atccatattc gtattcctgc tggagataaa aatgatcggt
     8341 actttcgata cttcacgtat tttttgacac caataatagc catccaaaac gggaagatta
     8401 atatctagta atacaagctg tggttcttct ctttcaaagt ctgagaacac ttgattgaaa
     8461 tcggtcgttc caaaggtttc aaattgccat ttctcaagtt cctccatgat caattgtcga
     8521 atgatctctt cgtcttcgac taccatgatt tttgccataa aaaatccctc catctttctt
     8581 cttgctacta caatatcaaa gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc
     8641 attttagcaa acgttaaaaa aaactggaaa tatgcgattt aatcgcgttt gtctcttcca
     8701 agcagcttaa atttattatg ttattcttaa taattggtct attatctgtt atttttcttt
     8761 cgttgttttt gtcttttaaa ttttcgataa caccaataac cgaaaagcgt tagcactagc
     8821 aatataccta gaaggagtat aagtaatcga taaagatggt atttttttgt ctgttctatc
     8881 tttgaagaga cttcttctgt aaagggaact cgtcttcctg tcactagtaa tcggtgagtg
     8941 ttcacggtgt aaggtgtaca agtgataagt gtgactagat cctgcttttc tctaattgct
     9001 aatgtatttg tttttgttgg aaggacaaca attttctcaa cgacttcata agcgagcatc
     9061 cgaccataca cttcaagata gaatcggtca tcttgctcca tttcttcaag atccgtaaag
     9121 agttttttct cagcaatacc gctatgggcg gtaatcacgg aatgtgtact ctctcctcca
     9181 gttggaaaag aggtgccttg caggactgtc gctcctctat ctaacaaaag atcattcgtt
     9241 tcatcaaata aaggcaagct aacattgatt ttcggaatat agatggctcc gatcatatgt
     9301 tgttcgtaat attcttttcc tggattttgg acatttctaa ccgcttgctc aaaaggatct
     9361 ttgacttgtc ccattcctgg gattactgat gtatgagcaa gcgtctgatt tttcttttca
     9421 tgcactgcta attgttgctc atgtttttct tctttatcgc gttcaagctg cttttgataa
     9481 ttctcgatcc ttctttgatc caaataattg ctgagagcat ctgctaaaaa tggatatgta
     9541 aaaactgcag ctccggcaag aaacatcact gccataaata attgtaataa aagactgatt
     9601 tgggtttgag gtctattacc tattttcttt ttcattttct tcaccttctt atttaaaaaa
     9661 actgcagcaa cctttataaa accgattgct gcagcttatt tttgttgctt atacttcaga
     9721 cttcatttta gatttacggt accagctgta cgctccaatc atcaaactta ttccgattag
     9781 taagaatgcc aagagcccgt tcccaccagt agcaggaagt gacccgcgtt tcatattcgg
     9841 tactctttct ggccctggat tttctactcc tacgatctga atttcttcat tatagcttcc
     9901 ataagagatg ataaattcag taaaggctgc ctcacctgtt ggaagtacat aaccttctgg
     9961 cgccatggtc tctttcattt gataggttcc ataatctaac ccttgaacct caaagtatcc
    10021 atttccatct gcgacaaatt ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc
    10081 tcctgattca taaacagcta aaggcgactc ccctttataa aatattgcgt attcagaacg
    10141 gtcttcattc cacaattcga atcttgcatc tttaagcaat tcactgcttg atgcatcata
    10201 tttttcaaac tttcttccac ctgttgtgac tttctctggc ggctctactc cttcttcata
    10261 atcaaattca tcatttcgtc cgatttcaat aaccgctgtg ttgttatgga aatcatccgc
    10321 ttctagttct gtggaaacct tcaaattata cgtgatcgtt aaatctcctc cacgtgcatc
    10381 tgctaatcta cctaaagtat ctactttggc tactccattt tcttctgtca aaatcgtcac
    10441 gacaaacccg ttgcttacta cttctactat ataatcttgt ccttttgtta aaatcgtctc
    10501 gccggctcgc acttctattt cctgatcata ataagttaga ccaactgtag gcgtatctgt
    10561 gattttgtaa tttttgataa cagcgcttcc gttttcaagg acagaatcga tgaaataagg
    10621 aataggtgct gtgatcgtgt aagaagccgt ttttcctctt tctagatcaa tataagaaat
    10681 cgtttcttgt tctccagctt cattaataat cgttacttgt ctaaggtcgc tttcctctac
    10741 attcatctgt ttatctgctt ctctgatttc gtttttcgga tacaaatgca catctgtatt
    10801 gtatgtgtct ttccatgtag aaccatccca cacgccatcc accatatcag gcatcatcac
    10861 tggcatcata acaaccattg gagacgcgat tctggaaata tgagctggcg aataagtttc
    10921 taagaaaaga tagactttat ctctagaaga aggtgcttct tccgtcactt ggacggtaga
    10981 gaataccgct tctccagctt cattcgttgt ttgtttgtca atacttgtca gtcgatattt
    11041 taaaaaccaa ctttctgtgt gccgtttttg aatccattca atcgcttcgg cagaattaag
    11101 accgtcttct cgttccggtg ttaaaggatt atctcgaatc aaatcgtaat atacttccgt
    11161 tacgtcaaac aaatcaaaat cgactcctgg aatattttct tcaccaaaat ccgtagaaac
    11221 caatccgcta ttcggtcgtt cttccggaac agatgagaaa ccttttttgt gtaatgtaat
    11281 cgtcacttca cttggttttt gtgcgacttc ttcggctttg actagttttc cgttcccgaa
    11341 aatccctatt cccgcagtaa cgagtaacag tacactcatt cccgtgacaa tcattttttt
    11401 attcattttt ttctccccgt tcttttttta tt
//
LOCUS       DAYYOA010000085        11219 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000085 DAYYOA010000000
VERSION     DAYYOA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.086"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHBKA_002042"
     CDS             136..309
                     /locus_tag="ACHBKA_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445711.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACHBKA_002043"
     CDS             949..1422
                     /locus_tag="ACHBKA_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM9445712.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACHBKA_002044"
     CDS             1431..1658
                     /locus_tag="ACHBKA_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445713.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACHBKA_002045"
     CDS             2294..2665
                     /locus_tag="ACHBKA_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445714.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACHBKA_002046"
     CDS             complement(2921..3163)
                     /locus_tag="ACHBKA_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9445715.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACHBKA_002047"
     CDS             complement(3195..4097)
                     /locus_tag="ACHBKA_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9445716.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACHBKA_002048"
     CDS             complement(4110..4298)
                     /locus_tag="ACHBKA_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9445717.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHBKA_002049"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHBKA_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9445718.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACHBKA_002050"
     CDS             complement(4913..5806)
                     /locus_tag="ACHBKA_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9445719.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACHBKA_002051"
     CDS             complement(5884..6807)
                     /locus_tag="ACHBKA_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9445720.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACHBKA_002052"
     CDS             complement(6856..7206)
                     /locus_tag="ACHBKA_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9445721.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACHBKA_002053"
     CDS             complement(7239..8141)
                     /locus_tag="ACHBKA_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9445722.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACHBKA_002054"
     CDS             complement(8134..8991)
                     /locus_tag="ACHBKA_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9445723.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ACHBKA_002055"
     CDS             9325..10134
                     /locus_tag="ACHBKA_002055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9445724.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ACHBKA_002056"
     CDS             10174..10671
                     /locus_tag="ACHBKA_002056"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM9445725.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ACHBKA_002057"
     tRNA            complement(11056..11129)
                     /locus_tag="ACHBKA_002057"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
BASE COUNT         3293 a         2097 c         1879 g         3950 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattg
//
LOCUS       DAYYOA010000086        10967 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000086 DAYYOA010000000
VERSION     DAYYOA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.087"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..1554)
                     /locus_tag="ACHBKA_002058"
     CDS             complement(91..1554)
                     /locus_tag="ACHBKA_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9445726.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1618..4635)
                     /locus_tag="ACHBKA_002059"
     CDS             complement(1618..4635)
                     /locus_tag="ACHBKA_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9445727.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4786..5625)
                     /locus_tag="ACHBKA_002060"
     CDS             complement(4786..5625)
                     /locus_tag="ACHBKA_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFM9445728.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5770..6015
                     /locus_tag="ACHBKA_002061"
     CDS             5770..6015
                     /locus_tag="ACHBKA_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445729.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6078..6500
                     /locus_tag="ACHBKA_002062"
     CDS             6078..6500
                     /locus_tag="ACHBKA_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445730.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6594..6911)
                     /locus_tag="ACHBKA_002063"
     CDS             complement(6594..6911)
                     /locus_tag="ACHBKA_002063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445731.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6938..7144)
                     /locus_tag="ACHBKA_002064"
     CDS             complement(6938..7144)
                     /locus_tag="ACHBKA_002064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445732.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7125..7307)
                     /locus_tag="ACHBKA_002065"
     CDS             complement(7125..7307)
                     /locus_tag="ACHBKA_002065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445733.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7321..7671)
                     /locus_tag="ACHBKA_002066"
     CDS             complement(7321..7671)
                     /locus_tag="ACHBKA_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445734.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7697..7999)
                     /locus_tag="ACHBKA_002067"
     CDS             complement(7697..7999)
                     /locus_tag="ACHBKA_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445735.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8002..8235)
                     /locus_tag="ACHBKA_002068"
     CDS             complement(8002..8235)
                     /locus_tag="ACHBKA_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445736.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9139..9669)
                     /locus_tag="ACHBKA_002069"
     CDS             complement(9139..9669)
                     /locus_tag="ACHBKA_002069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445737.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(9666..10781)
                     /locus_tag="ACHBKA_002070"
     CDS             complement(9666..10781)
                     /locus_tag="ACHBKA_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9445738.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10787..>10967)
                     /locus_tag="ACHBKA_002071"
     CDS             complement(10787..>10967)
                     /locus_tag="ACHBKA_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445739.1"
                     /translation="ILLNVSEEEIEEIKNELEDYDLLTIEFGRIYLKELIATMEDVRV
                     YQHQNSFLIYGQDEV"
BASE COUNT         2866 a         2283 c         1693 g         4125 t
ORIGIN      
        1 aaaaacgaaa atgagagata caaatatatc aaatatttaa aaagtaacca cattttctat
       61 accactcctt atcagaaaca gccttattct ttagaataag actgtttctg ggttgattaa
      121 tttgtctttt gttaaactac catctaaggc aagttctaaa tgtaaatgtg gtcctgtact
      181 atttcctgtt gaacccacat aaccaataat ttgtccttgt tcaactgttt gtcctacgtt
      241 cacgcaatat tcctgttggt gcgcataaag agccacactt ccgtcttcat gctcaattgt
      301 gacataattt cctgacaaag tatgatacga agcttctatg accgttcctg ccttgctcgc
      361 atagattggt tctccttgat tggctgccat gtctaatcct ctatgaaatt cttctcctcg
      421 ccaaccgaag gaactagaaa ttgaataatt ttttagaggt accatatagc cttctttgct
      481 tagttgtgga ccagctactt ctttatcata tttgattaag tcataggttt cgattaaccc
      541 atttaatttt ttatcgtagc tagtgtctgt tgcgtatctt cctgttaaaa atttagtcgc
      601 ttcctgatag gtttttgcct tacttttcca aacaccatca taaaaagagg tatttccaac
      661 taagccttct tttaacagtt tggcatagtc ttttaagctt tcttcatagc tctcatattg
      721 ccgaaaagtt gcttgggtcg tataaagtgt cccattgcct aaatcttctt gtgtcgcaaa
      781 agagacgccc tttccgttat gtgtgccttt gattccaaat agattgtagt ttggtgcctg
      841 agccaattga ctttgtccgc ttgcggattc taaaatggct tgcgcgatca taacagaagc
      901 gtataaatca ttttcttgtc caattttacg agcagattca ccgattttac ggataaacgt
      961 ttctacagac gcgtcttttt caaaatgaat agtcccttcg ttatcttgtg tgctggaagg
     1021 gactaattct tgtatcggtg cgcttggtgc ttggtggtag ttttttgtct cttctgaacc
     1081 gattgattgt gcctgcggta cgtcattcat aggtacagtc acactctgtt caggtgcctt
     1141 ttgtgggcgt tccgtttttg ttggcggttc cgcttctgtt tcggtacttg gtttttgagg
     1201 agtctgtgag ctttcatcag aaggttgctc atttggttct tctgacggtt cctcctcagg
     1261 ttgttgattg tcttctggtt tgtctggttg attcggtgtt tctggttgtt cttcagaacc
     1321 tgttggtgga gcgttcattt ctggattatc tggtgtatct attgtatctt cagtcggtgt
     1381 ttctggttga ttttcttctc cagtggaatt tgttgtcgtt tcttcagtga tcgttgcaga
     1441 ttgcgaattt gtcagttgat tgttgccttg ttcattagcc aaaaccgctg gcgtataccc
     1501 aaatacttga cctactaata acaaagcaga taaacattta ctcgtttttt tcacaagatt
     1561 gcctccattc ttcaatcaat catctagaga cagctgattg ctgtctctag tcatacttta
     1621 attgctttgt ttcattttac gacgaaccag aacagccccc acaccaacaa aaagaaacag
     1681 tcccaatgca attaacacat cgttcactgt ttctcctgtt tttggtaagc tttgttttgg
     1741 tgtgctcgtc ggcgtttgtt tgacaggggt tgtcggcgtt tcttttttaa tgccttttgc
     1801 tgtcaatgtt tgtttttcat tcttcaaatc aaagtgacgt gcttcttcct ctttatggtc
     1861 tttatcctta tagacaatat gagttgcgac caatttctcc ccgtctttta acgtgcctgc
     1921 tttgtagtca aaggatactt taaactcttc agacgcttta gaaactttac gtgcagtttc
     1981 taccgtacca acgactgtat cttttccatt tttatcaatc ttatggaatt gagtcaccca
     2041 gtatttggtt tcccctaccg tagattctgg aaattcttgc gatacatgat cgatcaactc
     2101 ttggtctttg tctggatcaa atgtttggtc gccattcact gtaataaagc gtgtttcgat
     2161 ttttggttct ttcgcagtca ctgtttgatc tttattggtt aaatcaaaat gacgagcgaa
     2221 ttcttcttcc tcttctttgt cgttatagac aatgtgcgtt gcgacaatcg tgtcaccgtc
     2281 tgaaaccatg tctgctttgt agttgaacgt cacatcaaat gtttcttttt catcggtcac
     2341 ttttcgtgtt tcttttaccg tatctaatac agtgactttt ccatcggcag taattttatg
     2401 gaattgcgtc acccaatatt tctctgtccc aaccgcagat ttagggaata cttgggacac
     2461 tttatccact aattcttggt cttttttcgg gttgaaatct tttccacctg tcgttgtcac
     2521 aaacaaagta gaaatttctg gttggtcttc ttctggaacc acgatttctt cgtttttcac
     2581 agtaattggt tgtacttctc cgtctttgac cgttactaaa atcgtttctg tttgtcctga
     2641 cgcttgccca taatgctcag ggaacacaat ttccgtaaat ttatacgtac cgtctggtaa
     2701 ttttagtttt tctggttctt cttttgtttc atacacaaag gacacatttt tattatgtgc
     2761 agtcatacct tcaatcagga atttcgctcc tggtaattgt agatgattga ttgaatcgac
     2821 tttattgaag tctacttcac ccgtttttaa caagttgtca atcacatcta attcaaacgt
     2881 aagcacaggc gtcgtgttat cgccataggc cacagagaac gtggtttgtt cttcagaagg
     2941 ttgacggtag ttgtctggca catttttttc aatcaattgg tatttgtttt gatcgccatt
     3001 gtagaatttt ttgtttgcct tccagtgtcc gtctttgtca gtttttactg aatccaagac
     3061 ttctcctttt ttcacaagga ctgtttcttt atctggcgca gtaatatctg ttttggcgat
     3121 aatatcaaat tcgatatttt ctgcaggaac ggtatcataa acaggtttgc cttgattcca
     3181 ggattcttcc gcattataga cttctttgac tttctttcct tctggaatga actgttgtaa
     3241 gtcatttttt gcagttaacg tcacttcttt gtttggttca atcgtaatcg tatgagtttg
     3301 tttgtctaga atatacccag ctggtgcttt gacttctttg acatccaatt tattttgaac
     3361 atattttccg tctttttcat agaatgcagg gatttctttt aaggcgattt cccctttggc
     3421 atccgttgta tattgttttg tttctcccgt atcatgacgt ttgatttcat acgtcacatt
     3481 tggaactttt ttccctgtga cagcgtctag tttggttagt ttgaagtcgc cattttttag
     3541 aactttaata gttagtccag ctctattagg atctggcaca taaagctttc ctagattttg
     3601 agcaacagga tctacatagg caaaacttga acctacccct ctagaatttg gatccagtga
     3661 agaaacagaa attcgtccgt catttgagtt ttgatttgct tttataaaca aactatttcc
     3721 tgattttctt acttgaacgt tagctgtgtt ttcaacaacc gacccatccc attgagaaaa
     3781 cacacctttt gtatccttaa cttctacttc ttcccctact ttaaccgtca ctgtttggct
     3841 atcaaaagaa ggatcttcaa gtaaattgtt tatttttcta tctacagtct gtttgaaagc
     3901 ttcataatta gattgtccaa tagttccata attaacaaat gaaccaccta aaacttccca
     3961 aatatatgtt tgtgctaata catagttatc cattgaacca ctagaatagt agccaaaata
     4021 accaattaaa gaaatctttt ttctaatagc acttgtcgta attaaatttt cgagtggtgt
     4081 cacatcatat tgaatacccg cactcgtgat tgcgccttct tgaatgcaaa aaacatcttc
     4141 tccgtcaatt tgccagcgaa ccatgtaatc atgagtatac attgttcctt caggagtaat
     4201 tcgagtcata ttcacatctt tacgaccatt ttcagtcaat gttagattgg tagcacgtgt
     4261 ctgtgcattc ttttccaaga ttgcttgcca ttcttcagat tcttgcgcca atcgttcttg
     4321 ttcagaaaaa gaagtcgctt tttgaatatc ttcaatggtt ataaagtatt tctcataacc
     4381 ttcagggacc tcttgagtca ttacattggg cggagaaaca tcttctgtcg tgtcctgcgt
     4441 agttgtctct tctgttgttt cacttgtggt ttcagtactg atcgcttctt cactactttt
     4501 tgattcttca gtcttcatta cttcttcagt agaatttgat tcatttatcg tttctgcaaa
     4561 tgccgttaca gatcctaaca tgggattgag aaatgtaacg aaaattgcca accaagttgc
     4621 cattctttta aacatatttt tttcgttcct ttcattttat ttttcagtct tctaaagtaa
     4681 tttcttatgg acttcgacac cgaaacattt ttatcctcct caaaacaaaa aaagactaga
     4741 acttataatt ctagtcttca tcaaccactt acttttcttt tatttttagt agaaatctac
     4801 agtgtgtttt tgtgacccgt cactgtaaat aataacatcc accataaaac ctttgttgta
     4861 ccactgcgaa tttggatcgc taacctctgc attagcccaa tcataggctt catctacagt
     4921 gttaaatacc tttccactat taccaatctc tccaactaca attgttggct cttcaggtgt
     4981 cgttgtgcca ccatttccgc cttgatcgcc tccaccagga ttgcttggtt cttcaggcgt
     5041 tgttgttcct ccacctgtgc ttgggttaga aggagttgtt tctcctccag tatttccacc
     5101 attacctggt tgtgtaggtg tttgagtagt gccaccattg ttttctggtg tacttggcgt
     5161 tgttgtacca ccagttgttc ctgtttgatc tccagctggt gttgttggtg tcgttgtatt
     5221 tggtgtcgtt tgtgtatttg aagtcgttcc ttgtcctact ggttgattcg gatcaacttt
     5281 gtctttctct tgaatcactg tttgagccac agtttcacct gcgctgtttt gtacacttgc
     5341 tacattgcca ttaaatacta atttatttcc tgcatagatt aaatccacat ttgtaatctt
     5401 attcatgtcc gctaattttt ggacagtaat atttgttgct tgactgattg cgcttagggt
     5461 atctccccag acaattgtgt attcattccc tttaatttct gctttgattt gttccgccga
     5521 acgaggtgtc caaactttgt tttctgctgc ttcagcattc gttcccacag caagactaac
     5581 tcctccgact acgattccta gtgccaatac tttcaacatt ttcattgttt ttcgcctccg
     5641 atttgaataa atttatttac aaaaacatag taccaaactt atgtctatca gtcgatatat
     5701 tttttgtaga tttttttatt ttttgatata ctaactttgg tactaaaata actaaaagga
     5761 atgtcacaca tgcaacttaa taatttaaat gaaatacatg attgggctca aaataattta
     5821 cttaatcgta aacaagctgc caaaatcact ggacaaaact atgatgcttt ttctagctct
     5881 ttacgtagaa ataatatcaa accatttcta gaaataggcg acgaacctgg tccttccatc
     5941 attcgcttat atctaagaga agatattgaa aaatacgcaa aacagctcga agaaaaaaga
     6001 cagaaacgtc aatgaggcgt tttctgtctt ggatttacaa gtaaaacaat aataaacact
     6061 tttacaacgg aggttctatg aataagaaat acattgtttc agttggttca atcgtactga
     6121 ttgtactcgc tattattgct ttttctgtat ccaacaaaac agaaacacag ccacaaaata
     6181 cggttcaatc gactactaca agtcaatcta gcgatcattc cgtctatcct tcactaaata
     6241 gtttaaaaga aacgcatgga caagatttaa ttgatcttgg ttcttctcaa attactactg
     6301 tcttatttga aaagaaaaat attgcgttta ttactcctaa agaaggaact gaccttgaat
     6361 tttctatcaa agtaacaaat accaaagaaa atttattctc cattcctgac attcaatata
     6421 cccctaacaa gttaaagatt ggggatacgt ttggtttggc taaagtaaat gaacaatact
     6481 ttgcttactc actgaaataa gtttctaata gcaagaggaa aaaccctctt gttattttta
     6541 tcacataatc attttataaa aacgcttcga aaatatcaac atttttgaag aaactatttt
     6601 tctaatttct caatttcttc caagggattt tcgctaatga cccatacagg cacagattgc
     6661 tctcgaggct ccccttcttc taaccaccaa cctgcataat gaacttcttc ctcctcagca
     6721 ttaccatgat accaaccaac aaaggattca tctacaacga atgaaatttc ttcttctgga
     6781 tcaaataatt catttttcca tgcttcaatt gcttgttctt tggtgtactt ttgtttgtta
     6841 aatacaccac aaatttcgtc atctacataa aaaatttcaa aatcaaaatt actcgttcct
     6901 tttactggca tcttaacttt tcctcctttg actcacttca atagatatac cactcatgtt
     6961 cattccattg tttactagtt aattggagtt ttatcttagg gctttgtgca ctctttccaa
     7021 aaacttgagc gtcgtttttt tcttctaacc aaaaaaggta atttcttaca ttcggtacta
     7081 aatgtttttc ctgcatttct tcatgaaaaa catataattc tgcttcattt ttcaaccaat
     7141 tcattattca ttccctcatt cacttcttca taccgccatt cagatagtga gatttctttt
     7201 tgtttctcca cttgtcctat aaaaatcgct gtattcaaca agctgatcac acttaataat
     7261 aaagcaatga aactaatggt tacacttact ctttcacatt ttgtcatttt gtttccttct
     7321 ttactcgttt gttatttctt gtaataggtc taataactcc ttcaccgaaa tttgcaaatg
     7381 gttcgcaaaa atttcaataa aagcactcgc acacattacc agaccttcaa ctgatatatc
     7441 actgcgacaa taaaattctg gagaaacact gatttctgat tctttcttac aattgaacga
     7501 aaaaactatt tttgaatcaa attccggtaa ttcttccaca actatgtttt gggattctac
     7561 caagaaaggg agtgcctgtt ctaatgcttc ttctggtgtt tgagcagata gaataattgc
     7621 ttcttgttct tttatttcat tgttctcaaa tactttataa ctttttttca ttgttagttc
     7681 ctctttcatt cctctctcaa ttagctaagc cgttactggt acgccattct ctaaatttag
     7741 tatcgctaat ccttaatgct cgtcgaatat caatcattcg ataacctatt gcacgaagtt
     7801 ctttatatct tttagcagtt agcgtctcta acgtcatttc aggcaatggg ttcgaatcaa
     7861 aaatagaatt cgaaccgatt atttgggcca attgttgtac ttgtaacagc tcttctgaac
     7921 ttaattcagt gattgctcga ttgcctatca ttgcatacat tttctgtcga atatacttct
     7981 tttcttgttt agacaacatt tctatccact cctattttca ttatcatttg ttacctccac
     8041 ttcagtaaat ccacaaataa atgaattcat attccaagta ccattccctt caactacaac
     8101 ttttctcctg tcctctttcg gatattcaag aatcagaccg tttaccaata tgtttttcac
     8161 tactactaat ttgtttgtat accgtggaat tgctgttccg atatacttca ctttatttcc
     8221 tacgctaatt gtcatttcct cactcctatt cttttttatc caaaactttt tcctgtccta
     8281 ttcctgccaa cgaatatact tcactgcatg cttaaacaaa acaaaatgtt gccctgtttt
     8341 atttccgtct tttacttgtt ccacatacag ttcatatttg gtcacacctt ttaaaacgcc
     8401 agatactttt tttcatccat tgtcacaatt agttttttca tcaatcgatt gatactgcac
     8461 tgctcatttt tcttgttcat atagtattcc tttctttttc aaaactagcc tcttttgttt
     8521 actagttttc atcaatcatc aacgatttaa cagatttttt ctttcgcagg gtttctagcg
     8581 tcagcaaccc caaactctaa aaaagtgcct ttgctccctt gaaaaacgag cggacgattg
     8641 tactcgtcat catatgtttt tcatactacc tgatccacgc aaataacctt tttttgttct
     8701 gtgccacacc catccatacg tatcgcttga gattcaccat acttttttag gtggtttatc
     8761 cttcctaagt ccctgttaga cacgctgatt aacttagaaa gtcctgtttg tatccaattt
     8821 tcaaagatct cccaaaagaa ataagtaggt ttacgtgtat cgcttctgtt caaagtacct
     8881 gtttgtactt gagtatttct actcccctcg ttcaagggat tccttccacc agctagcttt
     8941 tcctttcatc atcaaatgtt cctgaaaagc atttacgttt cgtgtccgtg tgcttactta
     9001 ttcttttttc gatagcgatt atccgctcgc tactcgctat tttatacttt gtttgttagc
     9061 tcaaataaaa actaacaaac aaaaaaggct agtttctttc cgttttattt ttttaactgt
     9121 cgataaatag ttgttagttt aattccaagt tctaaaacca atacgcccaa caaaaccaca
     9181 ggaaaaacaa ttggggcaaa caagtaaaat cggatagttt tcatgtcttc cctctttctt
     9241 tttggtgtcg ctgtggtcgt tcttgccgtt caatacttcg atacattttt tattcattat
     9301 ttgctcgttt tgttttcctc tggactgctt ttcttgcttt ttgaatactt tatacgcttt
     9361 tcgtgcctga attaataggt cttgtcggcg tttcattcgt gagaaaacaa tatttgaaac
     9421 catcaaatcc tggttcgaac aaccacgatt cgcaataatt ttcccatcaa aaaaaatagt
     9481 tttcacttga gaaaaaaaca gttggaaatc ttgttttgtt gctaattttt ttgtcaaaat
     9541 cactgtgctt ttccctgttg aatctacaat gattcttcga acaatccgat tcgataactt
     9601 ccaacgttgc cgattaaaca atgttacttc acaacaaata tgacgtaagt gattaggggt
     9661 cattttcatg tgtccaattc ctccaaccaa ttatgcatcg aaattttggg caattgtgac
     9721 agcccataaa tctgcgtttc caggtcttca taactacaag aacgcttggc ttgaagtaat
     9781 gaatattctg cttgatctga tagttttgtt gccattggat ctagattcaa ttctcttaca
     9841 caacattccc aaaaattcaa tagcgctcta aaataaaatc cttttcgatt ctgaattgct
     9901 tttccttcat gacgagcaat tttttcattt tgaatacacc gttgatacgt tttgcttaaa
     9961 tcttctgcaa atgtttcccc taaaattcga tactcgcttc catcagataa accatgtgtt
    10021 tgattatact ttcgttgccg cttttctata attcgtttac tgtcgtatat catgtttagc
    10081 tgctctttcg cttcacgcca ttcagctgaa ctgcgaacaa tccatgtcaa aacatctttc
    10141 ggaacaaacg tactttcgct gtaatgagca ataaactctt tttgccaatc atcagccatt
    10201 tcaacaggac tagtcgtatc gatttttgta tctagatttt ctgtatctag attgttagaa
    10261 gaattattaa ataattgtgc agaattttca catggatttt tttcgtcaaa atcatcgaaa
    10321 atcgctaaat tatcaacaga ttcctcctct tcttttcctt gtgcagaatc tacactggtt
    10381 gagtcaacag gacctaatga atacacatca ttttctgtcg tatctaattc tcctagatac
    10441 aaccgagctt tgtttctttt tcctgtttgt tgatcaaatc ccatatcctc tatttctagt
    10501 agctcatatt ctttcaattc ttttttgatt gtattaacag aacgtctgca acaattcaat
    10561 gcattggcta attcttgttc cgtaaattca aaataaacaa aaccttgact atctacttga
    10621 tttcgttgaa tggctaaatc taagcgtgac cgaaaaacca tatacgcgat ttttgcttta
    10681 tcagacaatg agcgatattt ttcacttgta ataaatacct ttggaaattg atagtataat
    10741 tcattgtatt tacgagttgc ggtaaatctt tttgttgcca tcgttattac acctcatctt
    10801 gtccatatat taagaaagaa ttttggtgtt ggtagacacg aacatcttcc attgtagcaa
    10861 tcaattcttt tagataaatt cgaccgaatt caattgttag taagtcgtaa tcttctaatt
    10921 catttttgat ttcttcaatt tcttcttcag acacattgag taaaatg
//
LOCUS       DAYYOA010000087        10922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000087 DAYYOA010000000
VERSION     DAYYOA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.088"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHBKA_002072"
     CDS             complement(<1..281)
                     /locus_tag="ACHBKA_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445740.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACHBKA_002073"
     CDS             453..1541
                     /locus_tag="ACHBKA_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9445741.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACHBKA_002074"
     CDS             1669..3045
                     /locus_tag="ACHBKA_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9445742.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACHBKA_002075"
     CDS             3446..5026
                     /locus_tag="ACHBKA_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM9445743.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACHBKA_002076"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACHBKA_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACHBKA_002077"
     CDS             complement(5652..5972)
                     /locus_tag="ACHBKA_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9445744.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACHBKA_002078"
     CDS             complement(6120..8354)
                     /locus_tag="ACHBKA_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9445745.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACHBKA_002079"
     CDS             8833..9099
                     /locus_tag="ACHBKA_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM9445746.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHBKA_002080"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHBKA_002080"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM9445747.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DAYYOA010000088        10752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000088 DAYYOA010000000
VERSION     DAYYOA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.089"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACHBKA_002081"
     CDS             complement(25..162)
                     /locus_tag="ACHBKA_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9445748.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACHBKA_002082"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACHBKA_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9445749.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACHBKA_002083"
     CDS             complement(1599..1853)
                     /locus_tag="ACHBKA_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445750.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACHBKA_002084"
     CDS             complement(1878..3020)
                     /locus_tag="ACHBKA_002084"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM9445751.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACHBKA_002085"
     CDS             complement(3080..4432)
                     /locus_tag="ACHBKA_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9445752.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACHBKA_002086"
     CDS             complement(4503..4847)
                     /locus_tag="ACHBKA_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9445753.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACHBKA_002087"
     CDS             complement(4857..5231)
                     /locus_tag="ACHBKA_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9445754.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACHBKA_002088"
     CDS             complement(5244..5558)
                     /locus_tag="ACHBKA_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9445755.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACHBKA_002089"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACHBKA_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACHBKA_002090"
     CDS             complement(8976..9638)
                     /locus_tag="ACHBKA_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445756.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ACHBKA_002091"
     CDS             10255..10485
                     /locus_tag="ACHBKA_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445757.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..>10752
                     /locus_tag="ACHBKA_002092"
     CDS             10491..>10752
                     /locus_tag="ACHBKA_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9445758.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYI"
BASE COUNT         2968 a         2377 c         1826 g         3581 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aa
//
LOCUS       DAYYOA010000089        10723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000089 DAYYOA010000000
VERSION     DAYYOA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.090"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..1089)
                     /locus_tag="ACHBKA_002093"
     CDS             complement(241..1089)
                     /locus_tag="ACHBKA_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9445759.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1253..2155
                     /gene="gnd"
                     /locus_tag="ACHBKA_002094"
     CDS             1253..2155
                     /gene="gnd"
                     /locus_tag="ACHBKA_002094"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM9445760.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="ACHBKA_002095"
     CDS             2157..3158
                     /locus_tag="ACHBKA_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM9445761.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="ACHBKA_002096"
     CDS             3155..3439
                     /locus_tag="ACHBKA_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM9445762.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="ACHBKA_002097"
     CDS             3472..4269
                     /locus_tag="ACHBKA_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9445763.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="ACHBKA_002098"
     CDS             4283..5116
                     /locus_tag="ACHBKA_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9445764.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="ACHBKA_002099"
     CDS             5134..5991
                     /locus_tag="ACHBKA_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9445765.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="ACHBKA_002100"
     CDS             6013..6522
                     /locus_tag="ACHBKA_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9445766.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="ACHBKA_002101"
     CDS             6559..6960
                     /locus_tag="ACHBKA_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445767.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="ACHBKA_002102"
     CDS             7072..7425
                     /locus_tag="ACHBKA_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445768.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="ACHBKA_002103"
     CDS             7459..7902
                     /locus_tag="ACHBKA_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9445769.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="ACHBKA_002104"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="ACHBKA_002104"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9445770.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="ACHBKA_002105"
     CDS             8997..9710
                     /locus_tag="ACHBKA_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9445771.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="ACHBKA_002106"
     CDS             complement(9917..10066)
                     /locus_tag="ACHBKA_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445772.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="ACHBKA_002107"
     CDS             10462..10662
                     /locus_tag="ACHBKA_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9445773.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1694 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa att
//
LOCUS       DAYYOA010000090        10623 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000090 DAYYOA010000000
VERSION     DAYYOA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.091"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..815)
                     /locus_tag="ACHBKA_002108"
     CDS             complement(<1..815)
                     /locus_tag="ACHBKA_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9445774.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISL"
     gene            961..1764
                     /locus_tag="ACHBKA_002109"
     CDS             961..1764
                     /locus_tag="ACHBKA_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM9445775.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1942..2889
                     /locus_tag="ACHBKA_002110"
     CDS             1942..2889
                     /locus_tag="ACHBKA_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM9445776.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2957..3250)
                     /locus_tag="ACHBKA_002111"
     CDS             complement(2957..3250)
                     /locus_tag="ACHBKA_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM9445777.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3278..3943)
                     /locus_tag="ACHBKA_002112"
     CDS             complement(3278..3943)
                     /locus_tag="ACHBKA_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM9445778.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4083..4715)
                     /locus_tag="ACHBKA_002113"
     CDS             complement(4083..4715)
                     /locus_tag="ACHBKA_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9445779.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4722..5789)
                     /gene="liaS"
                     /locus_tag="ACHBKA_002114"
     CDS             complement(4722..5789)
                     /gene="liaS"
                     /locus_tag="ACHBKA_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM9445780.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5786..6517)
                     /gene="liaF"
                     /locus_tag="ACHBKA_002115"
     CDS             complement(5786..6517)
                     /gene="liaF"
                     /locus_tag="ACHBKA_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM9445781.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6686..7165)
                     /gene="greA"
                     /locus_tag="ACHBKA_002116"
     CDS             complement(6686..7165)
                     /gene="greA"
                     /locus_tag="ACHBKA_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM9445782.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7301..8476)
                     /gene="mltG"
                     /locus_tag="ACHBKA_002117"
     CDS             complement(7301..8476)
                     /gene="mltG"
                     /locus_tag="ACHBKA_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM9445783.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            8680..9849
                     /locus_tag="ACHBKA_002118"
     CDS             8680..9849
                     /locus_tag="ACHBKA_002118"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9445784.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(10116..>10623)
                     /locus_tag="ACHBKA_002119"
     CDS             complement(10116..>10623)
                     /locus_tag="ACHBKA_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445785.1"
                     /translation="LILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYV
                     PIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTV
                     TVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIK
                     LKIRTSHE"
BASE COUNT         3280 a         2106 c         1640 g         3597 t
ORIGIN      
        1 aataaagaaa ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa
       61 tcaatatgtc cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga
      121 acatccttta ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca
      181 ggtgcacttt gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat
      241 atatatatcg aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg
      301 atcaaaattg ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa
      361 taactagatt ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac
      421 aaattcgcga gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct
      481 ggtgaagtga accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg
      541 aatgagttga tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta
      601 tttgcttctt ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca
      661 ctaaggttat ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata
      721 aattctgata aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat
      781 ctctgtggct gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag
      841 acaagaataa catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg
      901 tatttcactt gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt
      961 atgacaacaa aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg
     1021 agaaatgtcc cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct
     1081 ttgccaatct cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag
     1141 attgctactc gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa
     1201 gaaggattca tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga
     1261 acagaacagg acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa
     1321 aacaaactta tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac
     1381 caattagaac ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt
     1441 tcttttttaa gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct
     1501 atttatccta gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt
     1561 ctctatgcac gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc
     1621 tattcaattt cattgcctac tataattgca acaatcttga tggtctttct tccttctttt
     1681 gacactagcg ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga
     1741 tgggcaaaaa tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga
     1801 cccttttaga ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc
     1861 tttctcaaaa aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta
     1921 aagaaaggcg ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt
     1981 gatgaattta tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta
     2041 caaccagagg atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa
     2101 aaactattag cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag
     2161 ctaccccttg agactttttc aaagaaacat attttacttt caaatggtag tggcatccac
     2221 agtgaatcga ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt
     2281 caagcacaaa aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct
     2341 atccaaggga aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa
     2401 aaatgcaaag cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa
     2461 ggaatggaaa agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc
     2521 gtagtcaata tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt
     2581 gaacactttg atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca
     2641 caagatctgg tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt
     2701 gaggaagagc ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact
     2761 tcccacattg caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc
     2821 aatctgaaaa gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca
     2881 ggatattaat agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt
     2941 gtcaccctct ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat
     3001 cataatcaac aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa
     3061 aaagtgtccc atccatttcc actttcttta caggatgttc tggctgttca actgacattc
     3121 caatcgaaaa accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc
     3181 cgtaagtttc tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg
     3241 ttaatttcat cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta
     3301 gaatatccac atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt
     3361 catctgctgc aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta
     3421 agtcaaaacg ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg
     3481 agactcggat ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct
     3541 ttgacactaa attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat
     3601 cagctccgat cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat
     3661 ttggtacgcc catttccttt accatcagag taactaagat actggcttga atatcttctc
     3721 caatagcgac aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat
     3781 cctgtgcatt tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt
     3841 cattactatc aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc
     3901 cgccaaatcg tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt
     3961 atcttctttt ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta
     4021 aaaaagccaa gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt
     4081 ttttatttca ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact
     4141 tctagcttag ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat
     4201 tcgtccgcta tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc
     4261 cgattcgtta gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt
     4321 tttgttgtca cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca
     4381 gcaatttcat gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca
     4441 ggataaactt tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa
     4501 tcttttaaga ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt
     4561 aaaatgacgt ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct
     4621 tctccaatta cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc
     4681 atttcatggt catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa
     4741 gggaccttga tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca
     4801 ccaataccag ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca
     4861 ttcatattaa accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg
     4921 tgcaaatata cctctaattc attggcttta gcatgtctta atgtatttga taacaattct
     4981 tgaacgattc ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc
     5041 catttcagcg aaatctggat tttattttgc aattccttca ataactgctc gataccttgc
     5101 tttaaacttt tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca
     5161 gactgggaag cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt
     5221 gtctcgcttt tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat
     5281 tgttgcgaaa ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt
     5341 tcttctttgg tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta
     5401 gatacttcaa tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat
     5461 aaatcgtccg atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa
     5521 gaaagtgcac gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat
     5581 aatagaaaga caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga
     5641 acatataata aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa
     5701 aaataagtga aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct
     5761 agcatcgctc tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg
     5821 tattagtaat aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga
     5881 tttgttcatt tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg
     5941 catgctctaa tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt
     6001 ttcttacaat cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag
     6061 tatcaccaga aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac
     6121 gttggttacc gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg
     6181 tttggatcat catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg
     6241 aaatctctac acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag
     6301 caggactgtt gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt
     6361 gaaatttagt acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca
     6421 ctaaaagcaa aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg
     6481 cttcaactac cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca
     6541 ttctcatttc atcataccta tttttttaac tgttggctat aggactttag atgtaagatc
     6601 gtttgttgaa atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat
     6661 gaactctcta gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca
     6721 tatcgccacc tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt
     6781 gcgcaatagg agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga
     6841 caatcgtgta ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa
     6901 ttgatacctc atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat
     6961 tttcaattgt tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt
     7021 cagagttttc ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc
     7081 cgcgtttgac tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag
     7141 tcattggata aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa
     7201 aaggtaatct tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa
     7261 atgtttttct aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt
     7321 tttccactaa ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg
     7381 tatctgctac gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct
     7441 cttcgcttgg attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg
     7501 gtgaatctac agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata
     7561 aaacagagat atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg
     7621 caatgttttt acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta
     7681 aaacttcttg tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg
     7741 tattcgtctt gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat
     7801 catatgtggc tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg
     7861 cactttccaa cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca
     7921 ctaaatctaa aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct
     7981 gatcgatatc gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag
     8041 aagtaccgcc ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact
     8101 gataatagcc agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa
     8161 tcccgctttt aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc
     8221 cactcgggat ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc
     8281 tatctacata acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag
     8341 caatcactaa aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct
     8401 tgtcgattat atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt
     8461 gtttgtttcc tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata
     8521 catgatattt gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag
     8581 taaatccatt actataagat taaaataaat gttatcaaga tacaagctat tttagcttta
     8641 ttctttctgt aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct
     8701 aaaaaaagaa aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc
     8761 tctacaggaa ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc
     8821 catgctcaat cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa
     8881 aaaatcgatc aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat
     8941 aatcgcatca tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat
     9001 gatccttctc tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt
     9061 ttaaaacaag tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca
     9121 caaattgctg gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg
     9181 caccgaactg cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt
     9241 cagcacttaa aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata
     9301 ttgactggaa ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat
     9361 atcatcgaac ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc
     9421 aatcatgcac tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa
     9481 tctgaaacag agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat
     9541 ttaaatacgc tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc
     9601 tcattatggc gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat
     9661 aattatatcc tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa
     9721 gattcacagg atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg
     9781 cctactgctg atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca
     9841 aatagctgat atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa
     9901 aaagtactcg actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta
     9961 cagactttaa ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa
    10021 attattctaa gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat
    10081 agtatcttta gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt
    10141 aattttatgc aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt
    10201 ttagaaaaaa atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt
    10261 actgtaacaa aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc
    10321 gttaatcctt tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac
    10381 gtaaatgtaa tttcatcttt atttggagaa aagctactat aaataacttg accatcttct
    10441 gtaacttgat ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca
    10501 aaattctcaa attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa
    10561 ttcatttctt tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt
    10621 aat
//
LOCUS       DAYYOA010000091        10379 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000091 DAYYOA010000000
VERSION     DAYYOA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.092"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..531
                     /locus_tag="ACHBKA_002120"
     CDS             256..531
                     /locus_tag="ACHBKA_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445786.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ACHBKA_002121"
     CDS             544..2616
                     /locus_tag="ACHBKA_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9445787.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2784..4667
                     /locus_tag="ACHBKA_002122"
     CDS             2784..4667
                     /locus_tag="ACHBKA_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9445788.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4679..5626
                     /locus_tag="ACHBKA_002123"
     CDS             4679..5626
                     /locus_tag="ACHBKA_002123"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM9445789.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ACHBKA_002124"
     CDS             5646..6173
                     /locus_tag="ACHBKA_002124"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9445790.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ACHBKA_002125"
     CDS             complement(6236..6889)
                     /locus_tag="ACHBKA_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM9445791.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ACHBKA_002126"
     CDS             7021..7863
                     /locus_tag="ACHBKA_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9445792.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ACHBKA_002127"
     CDS             8021..8917
                     /locus_tag="ACHBKA_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9445793.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ACHBKA_002128"
     CDS             8920..9633
                     /locus_tag="ACHBKA_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM9445794.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ACHBKA_002129"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ACHBKA_002129"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9445795.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..>10379
                     /locus_tag="ACHBKA_002130"
     CDS             10168..>10379
                     /locus_tag="ACHBKA_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM9445796.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLE"
BASE COUNT         3599 a         1772 c         2151 g         2857 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaa
//
LOCUS       DAYYOA010000092        10283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000092 DAYYOA010000000
VERSION     DAYYOA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.093"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(309..2429)
                     /locus_tag="ACHBKA_002131"
     CDS             complement(309..2429)
                     /locus_tag="ACHBKA_002131"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9445797.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2659..3837
                     /locus_tag="ACHBKA_002132"
     CDS             2659..3837
                     /locus_tag="ACHBKA_002132"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM9445798.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3853..4611
                     /gene="aroD"
                     /locus_tag="ACHBKA_002133"
     CDS             3853..4611
                     /gene="aroD"
                     /locus_tag="ACHBKA_002133"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM9445799.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4634..5119
                     /gene="ybaK"
                     /locus_tag="ACHBKA_002134"
     CDS             4634..5119
                     /gene="ybaK"
                     /locus_tag="ACHBKA_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM9445800.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5190..6443)
                     /locus_tag="ACHBKA_002135"
     CDS             complement(5190..6443)
                     /locus_tag="ACHBKA_002135"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9445801.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6561..7910
                     /gene="gdhA"
                     /locus_tag="ACHBKA_002136"
     CDS             6561..7910
                     /gene="gdhA"
                     /locus_tag="ACHBKA_002136"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM9445802.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            8022..9368
                     /locus_tag="ACHBKA_002137"
     CDS             8022..9368
                     /locus_tag="ACHBKA_002137"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM9445803.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9676..10062)
                     /locus_tag="ACHBKA_002138"
     CDS             complement(9676..10062)
                     /locus_tag="ACHBKA_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9445804.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10111..>10283)
                     /locus_tag="ACHBKA_002139"
     CDS             complement(10111..>10283)
                     /locus_tag="ACHBKA_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM9445805.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         3302 a         1856 c         2090 g         3035 t
ORIGIN      
        1 gcagaaaagc ttgaaaataa acaatagagt attgtctgct ttttgtaata aattaatcga
       61 gcagtagcat agcggattat tttcatactt ttcacctcta taaaaagtat agaaaatgaa
      121 tatgaataaa gtatgaaatt cctttagtga aatcacgaat taaaaccaga aaatattttg
      181 taaaaagcaa gaggttgtga agaagctgcc ctattctatt accgtagatg caagcttttg
      241 aacactgttt attcgaaaag aggagcagcg acaataattt tgtcgctgct ccttttaaat
      301 ttattttgtc attcggaaga acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt
      361 ttataaagat caacagttga ttgtccgttg tgttcactaa agacactagt tgacttgtca
      421 ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc ataatcactg
      481 gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc attattgatt
      541 tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat ttcattctgt
      601 acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat cgtaccaccg
      661 tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag ctgttcatga
      721 tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac acctagtaag
      781 tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat tccgccctgt
      841 gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt gtcgtagttt
      901 gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct tgaattcgag
      961 acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc gctttctttc
     1021 aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc aaagtaaccg
     1081 ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt ggtgaatttt
     1141 gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg ttgcagacgt
     1201 tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa
     1261 ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc taaattcttg
     1321 tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc actagtgtaa
     1381 ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc gcctaattgt
     1441 gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt ttccgcatcg
     1501 cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg
     1561 tggtaaagac tattgatgaa aggtgtaact tcatgctgga gaccattctc atctgtcaat
     1621 ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac attttttcct
     1681 ttagcgagcc cgaacaaatc gtcatttggt gctgcataat gttcttttac ataattttct
     1741 acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg gtaagtttga
     1801 acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata atcacgagag
     1861 aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt tcctgagaaa
     1921 atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc
     1981 aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat ccaatagatc
     2041 acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc tactttccca
     2101 gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata agtgacattc
     2161 gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata agcaactcta
     2221 gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat agggttgata
     2281 aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac taaataggca
     2341 aaaatcgttt tcaaccaaag taaaacgact agtaaagaaa aaaatccgag cctcttattc
     2401 aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact tcttaatcac
     2461 agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt actagtatga
     2521 caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt tctttaagct
     2581 tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg ggaagagaaa
     2641 gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa
     2701 aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa
     2761 tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag
     2821 aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca atcttttttt
     2881 gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga tgattctact
     2941 accgcttttc gtatattcaa tggagaagga gacggaatcg gcggtctgat tgttgattat
     3001 tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta
     3061 ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga gaaaattcga
     3121 tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc accggaaccc
     3181 ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg attaatgact
     3241 gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt cgcttctggg
     3301 aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc cgcaatggga
     3361 ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa
     3421 cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc
     3481 gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt attggatcca
     3541 ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta tggggaactg
     3601 gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc tacaaatgct
     3661 gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca tgccaaaaat
     3721 gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac gacggatacc
     3781 tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag
     3841 gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag accaaagatt
     3901 tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt tcttgcacgt
     3961 gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt tttttctttt
     4021 gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact gccgttatta
     4081 tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc
     4141 gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat cgagttgttc
     4201 aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa cattccttta
     4261 attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct ttaccgattg
     4321 aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc caataatgaa
     4381 cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt ctccatgcct
     4441 attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg cggaacgacg
     4501 ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc aggacaatta
     4561 ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta gaacaagttt
     4621 gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga
     4681 acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc acctaagtgc
     4741 gtcatcggtc gctgaacagc ttccagaaag ccaatctcgt atatttaaaa cactggttgc
     4801 tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg aattgaatct
     4861 aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt taaaagattt
     4921 ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga aaaaactatt
     4981 tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct cggctggcag
     5041 aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga gcggacaatt
     5101 tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg gatgttgttt
     5161 gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt cattcctgct
     5221 acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc attgacatcc
     5281 agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt tttaggataa
     5341 atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg aaaagttttg
     5401 ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg tttttgagag
     5461 gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac taacaatcgc
     5521 tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt acttttttct
     5581 gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc
     5641 actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg cagtgcagcc
     5701 ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt caatacacga
     5761 ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg aagtgttttc
     5821 tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag aggcgtcacc
     5881 gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt agatggataa
     5941 gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa ttccgtgcgt
     6001 ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga gaatagaagc
     6061 gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt tgatttattt
     6121 gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg tgattcaaag
     6181 aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa ctttccattt
     6241 ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt gcaccgacca
     6301 ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt
     6361 gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc gccgactacg
     6421 atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat atctgaagaa
     6481 agaaaacaga aaaaaaatgt tttgagccaa aagagaaaat gagctaaaat gagggaagat
     6541 aagcaaagga gcgtggaacg atgacaaacg caacagaata tgttcaagca attcaagaaa
     6601 agctacatca aaaggatcaa gggcaagaag aatttttaca agcaatcgat gaattcatgc
     6661 ccacggttat gtcttttttg gacactcatc ccgaatatat cgaaaagaat atcttagaac
     6721 ttttaacgga accagaaaga gttattcaat ttcgagtgcc ttggcaagat gatgaaggaa
     6781 actggcgagt aaatagaggg taccgtatcc aatacaactc agctatcggc ccttataaag
     6841 gtggattgcg cttccatcct agtgtcaatc taagtatctt gaaatttttg gcttttgaac
     6901 agattttcaa gaatagttta accggtctgc caatcggtgg aggaaaaggc ggaagtgact
     6961 tcgatcctaa aggaaaatct gataatgaga tcatgcgttt ttgtcaaagt ttcatgctgg
     7021 aattgtcaaa acatatcggc ccatcgattg atgttcctgc gggagatatc ggtgtaggag
     7081 ctagagaaat cggctatttg ttcggcgaat ataaacgatt gaaaaaatat gatactggtg
     7141 tgttgacagg taaacctttg gatttttggg gaagtaagat acgtacggaa gctactggtt
     7201 atggattagt gtattatgtc aaacatctat tgaacgaaga aaaagattca tttaatcaaa
     7261 aaacagtttt tgtttccgga agcgggaatg tagccattta tgcaatcgaa aaagtgcaag
     7321 agctaggcgg gaaagttgtt acatgttcag attctagcgg gtacatctat gatccagaag
     7381 gaatagatgt gaaactattg aaagaagtca aagaaatcaa tagacaacgt ttgacggaat
     7441 acgcaaacca acgtccttct gctatttatc atgctggaga atctgtttgg acattgaaag
     7501 aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga aatagacgaa agcttggccg
     7561 aaatcttagt agaaaatggg atcaaaatcg tagcagaagg cgctaatatg ccatgtacag
     7621 taaaagctgt ttctgttctt catgaagcag gcgtgtggta ttgtccagga aaagctgcta
     7681 atgctggagg agtagcagta tctgctttag aaatgagtca gaacgtccag cgattgactt
     7741 gggaaagaga gcaagtggat gagcagctgg atcaaatcat ggaaacgatt taccatacgt
     7801 gtcgccaaac agcagccgag tacggcgacg aaaaaaatct gcttcttgga gcaaatgttg
     7861 ctggatttga aaaagttgcc aaagcaatgg cagcagaagg tttagtttga aaaatgaaac
     7921 tttcttggaa atattgtcag cattttgttt atttgcttga aaaataataa aaaaaaacgt
     7981 aaagtattac ctgtagagta aacctataag gaggagaaca aatggcacac attcattttg
     8041 actattcaaa agtagcaccg tttgtcaacg aacaagagtt agggtacatg caaagccaag
     8101 taacagcagc ccacaacgaa ttacgggaag gtactggcgc aggaagcgac ttccgcggct
     8161 ggatcgattt accaacgaac tatgacaaag aagaatttgc acgtatcaaa gaagcagcta
     8221 aaaaaatcca atctgattct gaagtattag ttgttatcgg tatcggtggt tcttatttag
     8281 gcgctcgtgc agcgatcgac ttcttgaatc acacattctt caatttatta gatagcaaca
     8341 caagaaaagc accacaaatc ttctttgccg gaaattcaat cagctcaaca tatattgctg
     8401 acttgatcga agtaatcggt gatcgcgact tttcagtaaa cgttatttca aaatctggaa
     8461 caactactga accagcgatt gcgttccgtg tattcaaaga gcttcttgtt aagaaatatg
     8521 gacaagaaga agcaaacaaa cgtatctacg ctacaacaga taaagcaaaa ggtgcagtta
     8581 aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat tcctgatgac atcggcggac
     8641 gtttctctgt attaactgct gttggtttgt taccaatcgc tgttagtggt gcagatatcg
     8701 atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata ctcaagcgac aaactagaag
     8761 aaaacgaagc ttaccaatac gcagcaatgc gcaacattct ttaccgtaaa ggcaaagtaa
     8821 ctgagttgtt gatcaactat gaaccaggaa tgcaatactt ctcagaatgg tggaaacaat
     8881 tgtatggtga atctgaagga aaagaccaaa aaggcatcta tccttcaagc gcaaacttct
     8941 caactgattt gcactcttta ggacaattca tccaagaagg acgtcgcaat atttttgaaa
     9001 cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat tcctgaacaa gcagaagatt
     9061 tagacggtct aggctacttg caaggaaaag aagtggactt cgtaaataca aaagcatttg
     9121 aaggaacatt gcttgctcat acagacggtg acgtgcctaa cttgctagtc aaaattccag
     9181 aaatggatgc ttatacattg ggttatacaa tgtacttctt tgaaattgct gtaggtattt
     9241 caggttactt gaatggaatc aacccattcg accaaccagg tgtagaagct tacaagaaaa
     9301 acatgttcgc acttctaggc aaaccaggct ttgaagatct tgctaaagaa ttgaacgaac
     9361 gcctgtaaga taagggtttt tcgctagatt taaataatca gtgatagaaa catgttcgct
     9421 ttaaaatgaa aactggtacc catttaggta ctcaaacata ctgcatcgtt tattcagaat
     9481 aaggggctgt gacaagacgt ttgtcacagt cccttgtttg tatagaaatc gattgattgc
     9541 cttgctcttt agtttgtttt aggactttaa gaacagtgag gctgacttcg aaggttaatt
     9601 ctgttttttt gttttctaga atcagtaagc aaaaaagcac tcacttcgag tgcttttttg
     9661 cgatatattc ttagcttaag ataggcctaa tttttctttt acagatgagg gtaccttatc
     9721 gctggtaatt acttttttct ctatgatccg cttttcgctt gctttcaact gcaagatcgt
     9781 tccttcgggg taagcagaac cattggcaga gtcaccagaa gtaatgacat cgaaatctaa
     9841 ctgtcttttt tcaccgtttt cagcataagc atctacgttg tatttaaaac ctcttccaat
     9901 tacttcccca ttatctgctt tgatatcagt ttctgcaggc attggagctt tgactaccgc
     9961 ataataatct ttacctacat aagtagactg atagtagtcc caacctttcc atcctataaa
    10021 agcaatcaat atgataacga tcaaccctaa aatttttttc atgaaagatc tcctttttta
    10081 tttttattat acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
    10141 ttcaaaacgt cgttgtgctc gacttaatag aacactgtaa gccagtaata tgctttcaaa
    10201 tctactctct agccctttaa cagaacgtga gttgaaattt tgacaaccta atttttctaa
    10261 ggaagaaaaa acagtctcaa ctg
//
LOCUS       DAYYOA010000093        10242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000093 DAYYOA010000000
VERSION     DAYYOA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.094"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..1544)
                     /locus_tag="ACHBKA_002140"
     CDS             complement(36..1544)
                     /locus_tag="ACHBKA_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM9445806.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1703..1894
                     /locus_tag="ACHBKA_002141"
     CDS             1703..1894
                     /locus_tag="ACHBKA_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445807.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2097..3014
                     /locus_tag="ACHBKA_002142"
     CDS             2097..3014
                     /locus_tag="ACHBKA_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM9445808.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3007..4080
                     /locus_tag="ACHBKA_002143"
     CDS             3007..4080
                     /locus_tag="ACHBKA_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445809.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4228..4746
                     /locus_tag="ACHBKA_002144"
     CDS             4228..4746
                     /locus_tag="ACHBKA_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445810.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4727..5119
                     /locus_tag="ACHBKA_002145"
     CDS             4727..5119
                     /locus_tag="ACHBKA_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM9445811.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5195..6130
                     /locus_tag="ACHBKA_002146"
     CDS             5195..6130
                     /locus_tag="ACHBKA_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9445812.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6263..7042
                     /locus_tag="ACHBKA_002147"
     CDS             6263..7042
                     /locus_tag="ACHBKA_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9445813.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7035..7352
                     /locus_tag="ACHBKA_002148"
     CDS             7035..7352
                     /locus_tag="ACHBKA_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9445814.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7596..9206
                     /locus_tag="ACHBKA_002149"
     CDS             7596..9206
                     /locus_tag="ACHBKA_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9445815.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9363..10211
                     /locus_tag="ACHBKA_002150"
     CDS             9363..10211
                     /locus_tag="ACHBKA_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9445816.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3218 a         1796 c         2068 g         3160 t
ORIGIN      
        1 tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa
       61 gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt
      121 ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa
      181 gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg
      241 atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca
      301 agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca
      361 tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact
      421 tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga
      481 atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg
      541 acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg
      601 aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca
      661 acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga
      721 taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca
      781 acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc
      841 atattgacat cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc
      901 aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt
      961 tggctttcat gatacagttg gttcaagcaa gtagcgagct ttccacttcc tgggccagga
     1021 gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct
     1081 tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc
     1141 ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg tccactatag
     1201 cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc
     1261 aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct
     1321 ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt
     1381 ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactaacttc
     1441 ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac
     1501 tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa
     1561 gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca
     1621 aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag
     1681 tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca
     1741 ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca
     1801 attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat
     1861 tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc
     1921 gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg
     1981 gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat
     2041 ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga
     2101 aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg aacgcggatc
     2161 aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc
     2221 tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc
     2281 ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg
     2341 aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat
     2401 ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca
     2461 cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt
     2521 tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca
     2581 aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag
     2641 atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct
     2701 tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat
     2761 ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa
     2821 aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag
     2881 aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg
     2941 aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt
     3001 ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat
     3061 catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa
     3121 gaaatcccta tgctagagga aacaaaaata catgaaaaag aaaaagtaac tccgacgaat
     3181 acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa
     3241 ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta
     3301 gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca
     3361 tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc
     3421 gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat
     3481 tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa
     3541 aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg
     3601 atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg
     3661 ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata
     3721 ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat
     3781 cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg
     3841 acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg
     3901 gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata
     3961 acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct
     4021 gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga
     4081 ttggataagt tggattacca gatgtcattc agataattct gttccaaagg acttgcggca
     4141 gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt
     4201 gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta
     4261 ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca
     4321 atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc
     4381 atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc
     4441 caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca
     4501 gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga
     4561 ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac
     4621 cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt
     4681 tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat
     4741 ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta
     4801 tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac
     4861 gttacaagaa aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa aaggcgatga
     4921 tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc
     4981 gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg
     5041 gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt
     5101 caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt
     5161 cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat
     5221 gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg
     5281 ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg
     5341 cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt
     5401 gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct
     5461 tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc
     5521 ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga
     5581 aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc
     5641 cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga
     5701 cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac
     5761 cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc
     5821 cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag
     5881 cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat
     5941 aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg
     6001 taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc
     6061 tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc
     6121 tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt
     6181 ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt
     6241 tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt
     6301 gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt
     6361 gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc
     6421 cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac
     6481 attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg
     6541 ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga
     6601 aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc
     6661 tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt
     6721 tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt
     6781 cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc
     6841 aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc
     6901 aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc
     6961 taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca
     7021 ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg
     7081 tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct
     7141 ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag
     7201 atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga
     7261 ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac
     7321 ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat
     7381 tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga
     7441 tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt
     7501 tattttagga attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat
     7561 gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa
     7621 cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc
     7681 cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg
     7741 ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga
     7801 ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt
     7861 ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt
     7921 tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag
     7981 ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat
     8041 atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg
     8101 tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca
     8161 tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta
     8221 tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt
     8281 ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac
     8341 taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga
     8401 tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat
     8461 tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa
     8521 cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa
     8581 tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga
     8641 ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa
     8701 gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc
     8761 tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag
     8821 caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga
     8881 tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt
     8941 gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc
     9001 gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat
     9061 tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg
     9121 gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta
     9181 aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa
     9241 cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta
     9301 atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac
     9361 acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc
     9421 atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag
     9481 gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta
     9541 ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag
     9601 ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat
     9661 tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca
     9721 ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt
     9781 cccttgatga aatacttgca catatcgatc gaatccgaga taatacctat gcttatctga
     9841 ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga
     9901 ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat
     9961 tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa
    10021 gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag
    10081 tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg
    10141 gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt
    10201 cagcgcaata attcctttta ttcaaaaaac ataccaaaaa ta
//
LOCUS       DAYYOA010000094        10193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000094 DAYYOA010000000
VERSION     DAYYOA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.095"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACHBKA_002151"
     CDS             120..626
                     /locus_tag="ACHBKA_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9445817.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACHBKA_002152"
     CDS             643..1542
                     /locus_tag="ACHBKA_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM9445818.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACHBKA_002153"
     CDS             1629..2513
                     /locus_tag="ACHBKA_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9445819.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="ACHBKA_002154"
     tRNA            complement(2705..2776)
                     /locus_tag="ACHBKA_002154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="ACHBKA_002155"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="ACHBKA_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM9445820.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="ACHBKA_002156"
     CDS             4319..5410
                     /locus_tag="ACHBKA_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM9445821.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="ACHBKA_002157"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="ACHBKA_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9445822.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /gene="pgk"
                     /locus_tag="ACHBKA_002158"
     CDS             6562..7752
                     /gene="pgk"
                     /locus_tag="ACHBKA_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM9445823.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /gene="tpiA"
                     /locus_tag="ACHBKA_002159"
     CDS             7856..8611
                     /gene="tpiA"
                     /locus_tag="ACHBKA_002159"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM9445824.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="ACHBKA_002160"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="ACHBKA_002160"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM9445825.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DAYYOA010000095        10157 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000095 DAYYOA010000000
VERSION     DAYYOA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.096"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACHBKA_002161"
     CDS             complement(17..538)
                     /locus_tag="ACHBKA_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM9445826.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="ACHBKA_002162"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="ACHBKA_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM9445827.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /gene="cdaA"
                     /locus_tag="ACHBKA_002163"
     CDS             4618..5505
                     /gene="cdaA"
                     /locus_tag="ACHBKA_002163"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM9445828.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="ACHBKA_002164"
     CDS             5502..6671
                     /locus_tag="ACHBKA_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM9445829.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /gene="glmM"
                     /locus_tag="ACHBKA_002165"
     CDS             6695..8050
                     /gene="glmM"
                     /locus_tag="ACHBKA_002165"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM9445830.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACHBKA_002166"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACHBKA_002166"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM9445831.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         3160 a         1935 c         2230 g         2832 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatt
//
LOCUS       DAYYOA010000096        10086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000096 DAYYOA010000000
VERSION     DAYYOA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.097"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACHBKA_002167"
     CDS             complement(247..759)
                     /locus_tag="ACHBKA_002167"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM9445832.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHBKA_002168"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHBKA_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM9445833.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACHBKA_002169"
     CDS             complement(3212..3655)
                     /locus_tag="ACHBKA_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM9445834.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKASEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACHBKA_002170"
     CDS             complement(3656..4444)
                     /locus_tag="ACHBKA_002170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9445835.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHBKA_002171"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHBKA_002171"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM9445836.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACHBKA_002172"
     CDS             complement(5559..6146)
                     /locus_tag="ACHBKA_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM9445837.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHBKA_002173"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHBKA_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM9445838.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACHBKA_002174"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACHBKA_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9445839.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACHBKA_002175"
     CDS             complement(9063..9998)
                     /locus_tag="ACHBKA_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9445840.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2779 a         2298 c         1745 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccgacgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAYYOA010000097        10084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000097 DAYYOA010000000
VERSION     DAYYOA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.098"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACHBKA_002176"
     CDS             162..392
                     /locus_tag="ACHBKA_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM9445841.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACHBKA_002177"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACHBKA_002177"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9445842.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACHBKA_002178"
     CDS             1803..1979
                     /locus_tag="ACHBKA_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9445843.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACHBKA_002179"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACHBKA_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM9445844.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="ACHBKA_002180"
     CDS             2474..2941
                     /locus_tag="ACHBKA_002180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445845.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="ACHBKA_002181"
     CDS             3026..3910
                     /locus_tag="ACHBKA_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9445846.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="ACHBKA_002182"
     CDS             4024..4260
                     /locus_tag="ACHBKA_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM9445847.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="ACHBKA_002183"
     tRNA            4348..4428
                     /locus_tag="ACHBKA_002183"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="ACHBKA_002184"
     tRNA            4435..4506
                     /locus_tag="ACHBKA_002184"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="ACHBKA_002185"
     CDS             4631..4801
                     /locus_tag="ACHBKA_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445848.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="ACHBKA_002186"
     CDS             5021..5728
                     /locus_tag="ACHBKA_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9445849.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="ACHBKA_002187"
     CDS             complement(5788..6432)
                     /locus_tag="ACHBKA_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM9445850.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="ACHBKA_002188"
     CDS             6559..6846
                     /locus_tag="ACHBKA_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445851.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="ACHBKA_002189"
     CDS             6954..7274
                     /locus_tag="ACHBKA_002189"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9445852.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="ACHBKA_002190"
     CDS             7355..8668
                     /locus_tag="ACHBKA_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9445853.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="ACHBKA_002191"
     CDS             8665..9384
                     /locus_tag="ACHBKA_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM9445854.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="ACHBKA_002192"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="ACHBKA_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM9445855.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DAYYOA010000098         9960 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000098 DAYYOA010000000
VERSION     DAYYOA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.099"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..3370
                     /gene="fss3"
                     /locus_tag="ACHBKA_002193"
     CDS             143..3370
                     /gene="fss3"
                     /locus_tag="ACHBKA_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM9445856.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3483..3797
                     /locus_tag="ACHBKA_002194"
     CDS             3483..3797
                     /locus_tag="ACHBKA_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9445857.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3810..4184
                     /locus_tag="ACHBKA_002195"
     CDS             3810..4184
                     /locus_tag="ACHBKA_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9445858.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4194..4529
                     /locus_tag="ACHBKA_002196"
     CDS             4194..4529
                     /locus_tag="ACHBKA_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM9445859.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4612..5952
                     /locus_tag="ACHBKA_002197"
     CDS             4612..5952
                     /locus_tag="ACHBKA_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9445860.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6030..6704
                     /locus_tag="ACHBKA_002198"
     CDS             6030..6704
                     /locus_tag="ACHBKA_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9445861.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6697..6861
                     /locus_tag="ACHBKA_002199"
     CDS             6697..6861
                     /locus_tag="ACHBKA_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445862.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6937..8079
                     /gene="dcm"
                     /locus_tag="ACHBKA_002200"
                     /pseudo
     CDS             6937..8079
                     /gene="dcm"
                     /locus_tag="ACHBKA_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8069..8359
                     /locus_tag="ACHBKA_002201"
     CDS             8069..8359
                     /locus_tag="ACHBKA_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445863.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8618..9802
                     /gene="mobT"
                     /locus_tag="ACHBKA_002202"
     CDS             8618..9802
                     /gene="mobT"
                     /locus_tag="ACHBKA_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9445864.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9799..9936
                     /locus_tag="ACHBKA_002203"
     CDS             9799..9936
                     /locus_tag="ACHBKA_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9445865.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3297 a         1679 c         2249 g         2735 t
ORIGIN      
        1 gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt cagtcaataa
       61 aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt tttttgtgct
      121 taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat gtgtattaac
      181 tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga ttacttttgc
      241 tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac atcaatatgc
      301 tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc cgcattatgc
      361 agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc cgatttacaa
      421 tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata aagccaaatt
      481 agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg tggcacagaa
      541 gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat cagatggtag
      601 tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg attatcagaa
      661 gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga ctactgtgac
      721 ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg ccgcaaacat
      781 tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt caaaagaaag
      841 tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct ataaaaaggc
      901 tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca caatcaaaat
      961 tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat cgggtgatgt
     1021 tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa aagacgtgac
     1081 tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa aagtaaccat
     1141 taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga ctaccaccat
     1201 taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag ggcaaatcat
     1261 tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt attccttagc
     1321 tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc aagaaatgac
     1381 cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt tggaattggg
     1441 aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt tcaaacctgt
     1501 aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta acgtaaaagg
     1561 gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag atactgggga
     1621 taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta ctgctaagga
     1681 tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg gaacgaaagg
     1741 ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg ttaaacattt
     1801 agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata gccttgatga
     1861 aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg ccgtgattac
     1921 ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct ttaaatttgc
     1981 tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta aagtgtcgcc
     2041 attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag cttataacga
     2101 gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg attatttact
     2161 tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa tccgttctac
     2221 ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca ttaccgaaca
     2281 agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga cgaacaaagc
     2341 attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag aagatagttt
     2401 aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg attctaccga
     2461 gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt atgtggtagc
     2521 gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa aagccaaacc
     2581 agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag gaacttggaa
     2641 aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct tattcaacta
     2701 tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga aggatgttag
     2761 cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt ctatccaaac
     2821 aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg tggatatgtt
     2881 tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt tcgaaacaat
     2941 tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg gcaagattga
     3001 ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag tagataccgg
     3061 aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata aagatgggaa
     3121 tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa caccaaaaga
     3181 agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc caagtggctc
     3241 tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa attccaatgt
     3301 tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt tctggaatcg
     3361 ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg cgcgaccgcc
     3421 tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa gaggaaaaga
     3481 agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag ttaagttatg
     3541 ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg attggtcgca
     3601 gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta gtagctgcgg
     3661 aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat ccccatttgg
     3721 ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg tatgttgatg
     3781 atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt gtcgtagatt
     3841 caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta cggaagcaaa
     3901 atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta aaatcctctg
     3961 cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc gagtttgctt
     4021 atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat tatgtttttc
     4081 gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg aagcggcagc
     4141 cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag attatggaaa
     4201 ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag ctgactgagg
     4261 aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata catcaaggtt
     4321 tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg ttaggagaag
     4381 aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag gcagaatcca
     4441 ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta aatcaattgg
     4501 ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact ttttgaaatt
     4561 gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa aatgtacaaa
     4621 ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct tggctggcta
     4681 ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag acaatttgat
     4741 atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga tttagtatgt
     4801 ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca cttttttctt
     4861 gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct agagaaccat
     4921 tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa tagtagccga
     4981 accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga tggattactg
     5041 tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa attggaaaag
     5101 aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga ctcctatgtg
     5161 gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga agtagtggca
     5221 gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc tttaccccat
     5281 atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac cattatcgaa
     5341 gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc agatttagct
     5401 gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc tgcttgtgtg
     5461 gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat gtctaactac
     5521 aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat ctttgatgaa
     5581 tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt gaataagctc
     5641 aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc ttgccaaagg
     5701 ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg cgtggctttg
     5761 ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa aaacttcttt
     5821 atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat tagtgagttt
     5881 tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa attagaaaag
     5941 atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta taatactttt
     6001 atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat ttagagtatg
     6061 caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc cccgaagttg
     6121 gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag gttgccattg
     6181 gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt cgcacccagt
     6241 ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct tggttggtag
     6301 agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat gcaacttttg
     6361 caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt cgagtgacga
     6421 ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa cgtgaaattg
     6481 cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa tcaagagaga
     6541 acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata ataaaagatt
     6601 atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta tttttaccac
     6661 tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga acaagtaacg
     6721 aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc tactgcaaaa
     6781 gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca acggaaatta
     6841 gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag agcaaaatgt
     6901 tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga tttattcgcc
     6961 ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat cggtttttgt
     7021 gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag taaggaggtg
     7081 gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg accagtggac
     7141 atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg gcaaagattt
     7201 tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct tctccgacct
     7261 aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg ggctacgttc
     7321 gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta ggtgcttaac
     7381 tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca ttctcgagac
     7441 gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga tcaaaacatc
     7501 agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt ctatggttca
     7561 gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact gccagcaagg
     7621 gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa tcgagtttat
     7681 gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa aatcatgatt
     7741 cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa tctggctttt
     7801 cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac cttactaaca
     7861 ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac acctagagag
     7921 tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca agtcaattca
     7981 gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat ttttgatatt
     8041 gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg gagaaattta
     8101 ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc gacggttaaa
     8161 atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa tgaatgagga
     8221 gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga ttgcggacca
     8281 agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt tggaagtaaa
     8341 tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct gaccgcggag
     8401 gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc gttagccgac
     8461 catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa taagtaagca
     8521 accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt gttaactcat
     8581 ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga atttagatta
     8641 tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat tggctacggc
     8701 ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa ccgcatctat
     8761 aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt tgcctttgac
     8821 gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa aaattattca
     8881 agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct tttactccta
     8941 tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag aagataaagg
     9001 tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt tactcgctca
     9061 aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg tgatgaaacg
     9121 cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc taacgaaaaa
     9181 atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc gttccgggga
     9241 acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg gcagtctcaa
     9301 gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca agttagggat
     9361 tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa atgaccgtgc
     9421 ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca cgttttccat
     9481 tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa ccaactggcc
     9541 cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc aattaacttc
     9601 cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac aagtcgcccc
     9661 tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata tccaggatat
     9721 ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac aaagtatggc
     9781 aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa agatattttt
     9841 ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa tgcaagtgca
     9901 gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg gacaaaattt
//
LOCUS       DAYYOA010000099         9916 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000099 DAYYOA010000000
VERSION     DAYYOA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.100"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..684
                     /locus_tag="ACHBKA_002204"
     CDS             205..684
                     /locus_tag="ACHBKA_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM9445866.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="ACHBKA_002205"
     CDS             717..2945
                     /locus_tag="ACHBKA_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9445867.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="ACHBKA_002206"
     CDS             3074..4717
                     /locus_tag="ACHBKA_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9445868.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="ACHBKA_002207"
     CDS             complement(4790..5335)
                     /locus_tag="ACHBKA_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM9445869.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="ACHBKA_002208"
     CDS             complement(5358..5843)
                     /locus_tag="ACHBKA_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9445870.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="ACHBKA_002209"
     CDS             complement(5833..6645)
                     /locus_tag="ACHBKA_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM9445871.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="ACHBKA_002210"
     CDS             complement(6784..8316)
                     /locus_tag="ACHBKA_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9445872.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="ACHBKA_002211"
     CDS             complement(8425..9456)
                     /locus_tag="ACHBKA_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9445873.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3300 a         1836 c         1905 g         2875 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt ctatggtagg agcaattata ttgagaatta
     9901 atcctttttt atataa
//
LOCUS       DAYYOA010000100         9862 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000100 DAYYOA010000000
VERSION     DAYYOA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.101"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(328..552)
                     /locus_tag="ACHBKA_002212"
     CDS             complement(328..552)
                     /locus_tag="ACHBKA_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445874.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            743..3520
                     /locus_tag="ACHBKA_002213"
     CDS             743..3520
                     /locus_tag="ACHBKA_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9445875.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3601..4020
                     /locus_tag="ACHBKA_002214"
     CDS             3601..4020
                     /locus_tag="ACHBKA_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9445876.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4032..4502
                     /locus_tag="ACHBKA_002215"
     CDS             4032..4502
                     /locus_tag="ACHBKA_002215"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9445877.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4519..5298
                     /locus_tag="ACHBKA_002216"
     CDS             4519..5298
                     /locus_tag="ACHBKA_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9445878.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5285..6106
                     /locus_tag="ACHBKA_002217"
     CDS             5285..6106
                     /locus_tag="ACHBKA_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9445879.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6123..7172
                     /locus_tag="ACHBKA_002218"
     CDS             6123..7172
                     /locus_tag="ACHBKA_002218"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9445880.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7185..8189
                     /locus_tag="ACHBKA_002219"
     CDS             7185..8189
                     /locus_tag="ACHBKA_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9445881.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8822..9841
                     /locus_tag="ACHBKA_002220"
     CDS             8822..9841
                     /locus_tag="ACHBKA_002220"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9445882.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3304 a         1641 c         2061 g         2856 t
ORIGIN      
        1 ctatggtttg gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa
       61 gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc
      121 aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca
      181 ataaaaagga tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt
      241 cctcattatg ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac
      301 agacaggaaa gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa
      361 aatcaagata aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat
      421 aaaacgagtt tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag
      481 ataagatccg atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt
      541 atccgatttc attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat
      601 aaaaactgga aggaactgta cgttttttag aataaaaatt ataagggata agtaggctgc
      661 tgtctataat aagatatatt tttcaattct ttattaaaga ttttattgac aaaatgaatc
      721 cgttttctat gatggaggag gggtgaataa gttgttgaag aaggaaattt tatgttactt
      781 agagaaccag acagcctttt tcgatttaga acatatgaac gaggtcttta cagcacaaag
      841 gttagctgat cattttgggg taaaaagaaa cacagtcagt cattatttga accagttaaa
      901 tgatgagcaa ctactagtca aaatcaattc tcgaccagtg atttatttcc acaaagaagc
      961 ttttgaaaaa caatttttta agttacgcac gaatttttat ttttctatcg atgatctcaa
     1021 agaggaacag ccttttttcg cgcaaccaaa agacttattc tcttttatga tcggatacga
     1081 cgccagtttg aaagaaagta tcgaacaaat caaaacagct ctttactatc cagatggcgg
     1141 attaccactt ttgattacag gcgaaagtgg cacaggtaaa agttatttag ttaatttggt
     1201 ctatcagtat tgtctgcttc atgatctgct agaagattct gcaccattca tcacttttaa
     1261 ttgtgcacaa tatgcagata atcctgaact tttgactagc aatttgtttg gacatgttaa
     1321 gggagcgtac actggtgcag aagaaaataa aaaaggggct tttgaagcag cagatggtgg
     1381 gatcttgttt ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa aactgttcac
     1441 ctatcttgat caaggtgtga tttatcgtat gggagaacct aatttgtcac gccggatcaa
     1501 aacacgtctc ttttttgcta cgactgaaga gattactagt catttcttaa caactttcat
     1561 tcgaagaata cctatccgaa tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg
     1621 gctagaactg atttactctt tttttctcga agaacaaaga aaaatgggga gccctttgtc
     1681 agtcagtgga ccagctttgt cacttttaac aagtcagaaa ttatccggaa acataggaga
     1741 gctgaaaaat atcgtcaaag tttcagcagc aaaagcatat gctgaacagc gtgaacagcc
     1801 tgaaatccat gtcacgatcc atcatttgca aaaagaacta cttgcgcagc ctattactaa
     1861 aaatacagca cagcaaggaa tctatattac gcaagaccag accttagagc aattaatgga
     1921 gaagcaacag cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc taattgattt
     1981 caagaaaaat aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag aagtgattca
     2041 attatttgat tttttgctgt ttgagaccag tagacaagaa aaacacgagc tgctggttta
     2101 ttggacacaa tctatccgag agacctttcg acaaatggaa actgcctatc aaatcaaatt
     2161 cgatggaaac agtgtctatg ccttgagtta ctatctctat cagcgaagca ctatcaaatg
     2221 gttaccggaa gaacatgata ttatccaact aatcaaagaa ttagaacaac aagtttctca
     2281 gtcttatcca tctagttatc actatgtcca acgaattctg gaattaagta agatcaaatt
     2341 agatattgaa gacacaagta tggatcggat tttactgacg atctatctaa aaaaagcgaa
     2401 atggtcaaaa gaaaaacgac tgccaaaagc gatgattgct gctcacggtt atgcaactgc
     2461 tagcagtatg gcaaatgtag taaatcgttt attacaagag gatctatttg aatcatttga
     2521 tatgccacta gatgtcacac cacagcagat tgcttcagaa atccttgatt attgcgaaaa
     2581 cagcgatgtt tcaaatggat tgatcatatt agtagacatg ggatctctga aagaaatcca
     2641 tcagtttttt aaaaaacagt tatccgtacc gcttttgatt ttgaataatg tgacgacacc
     2701 tttagcaatc acagtagggg aatgcctgca aaaaaatgta tcattagaag agatagcaga
     2761 agagactgtt cgacagatcc agccagaatg gcgactgctc tatccagagg aaaacaaacc
     2821 caaagcactc attactactt gcttcactgg aatcggtacg gcggtccact taagtgaact
     2881 gctagaaaaa agcttaccta cggcttgtca gttgaaaatc atcccatatg aatatcagca
     2941 attaaaagat aagaaaaaca gtgacccatt attttccata tacgaagtcg taggaatgat
     3001 tggcaccaca gatcctgata tcaccaatat cgactatctt tcattagaag acctcatctc
     3061 gggggaaaag atgtctgtac tagctgaatg gttggacact actatgaatg cttcagagaa
     3121 ggaaatattc aatcagcgca tcattcgtaa tttttcattg gaaaaagtac tggattccgt
     3181 gactattcta gatacagaga aggtgatggg agaaatcgat cattttatga gagaattaga
     3241 ggtgcagttg aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag
     3301 ctgtctcatt gaacgattga tccgacaaat gccaatcgaa acctatcaag gattagagaa
     3361 atggaagcag tgtcaaaaag aagggttatc tgctattaaa tctgcgttta gtgtcattga
     3421 agagcattat agtgtcatga tccccaattc tgagattgcc tatatctatg atattttgag
     3481 tgaagataca gagtttctct cgatagaaga agaattttga acataagcaa gttgttgcca
     3541 ctaattttgg catgcaactt gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa
     3601 gtggatagag aaattatctt agcttcccat ggaaaatttg cttcagggat attagattca
     3661 ttagagctga tctatgggaa aaatcattct attactgtat tcgattgcta tactgatgag
     3721 aattttgatt tagcggaaac agtaaggcag ctttttatga ggtacaaaga taaagaaatc
     3781 attgttctga cggatctttt tggcggaagt gtcaacaacg aattcttaca atatatcaat
     3841 cgggatcatg tctatctcgt tgccggattg aatttgccac tattgattga gctagtatct
     3901 cggttggacg ctgaagtaga gacaactgtt ttgatccagc aggtgctaac tgattcaaaa
     3961 aaaatgatcc agttttgtaa cgagcggctc aatcagaaaa tagaagaaga cgaattttag
     4021 gaggaagaaa aatgattgta ttgacaagag tcgaccatcg gttattacac ggacaagtag
     4081 ctttttcatg gacacaaaca ataggcgcag actgtatttt gattgctaat gatgatgtac
     4141 ctacgaatga gatacgaaag acaacgatca aattagccaa gccacaagga gtcaaactag
     4201 tgatcaaatc aattgatgac tcgattgccg cgttgaaaag cggggtgaca gataaatata
     4261 agttattcat cgtagtggaa tcgatcgagg atgcatataa gctggcagaa gcgtatccag
     4321 aaatcaaaac catcaactta ggagggatca aggcaaaacc tggtactcgc agcatcggaa
     4381 aagcggtaaa tattttacca gaagaggagg aacgcttgaa acgattaatt gacaaacaaa
     4441 tagaagtaga aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt
     4501 aagaaaggaa gaacctcaat gattgtacaa gcagtcttat taggaattgt tgcatttgtc
     4561 gcacaatcag aatatgcact agggacatcg ttgctttctc gtccgattgt gacggggctg
     4621 tttacaggaa tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc aactttagag
     4681 ttagcattta tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt
     4741 gggattttag ggacggcttt tgctattaca gctggagccg gtacagaaac cgcgctttta
     4801 ctaggtttgc cgattgctac gttgacattg atattgaaaa atatttactt agggcttttg
     4861 attccaatta tgaaccataa agcagacaat tatgcagaag aaggaaatta taaaggagtc
     4921 gagcatatgc acttgcttgc cggtatcggt ctttccttga tgcttggtgt tgtggtaatg
     4981 gtttcttata tggtgggaag caatacgatc ggaaatctat taaatatgat tcctgacttc
     5041 gttcaaaagg ggttgtcagt agctacaggg ttgattccag ctcttggttt tgcgatgctt
     5101 gctcgtttgc tgatcaacaa acaagttgca ccatattttt tcttaggttt tgctatagct
     5161 tcttacttag agattcctgt gaccggagta gctatttttg gtgcgatttt agcagttgtg
     5221 gtagtcaata ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa
     5281 gaagatgaag aattctgaga tcaaaaacag caaaataacg aaaaaagagc taaatcacgt
     5341 attttggcgt tcttttcaaa tggagttttc ttggaattat gaacgtcaga tgaatatggc
     5401 ttatgtttat gcaatgattc cgattttgaa aaagttatat cagtcgaaag aagaaatggc
     5461 agcagctttg aaacggcatc tagagttttt taacacgacc ccacatatcg ttacactgat
     5521 tttaggaatc acggctgcaa tggaggaaga aaataaggat gatcctgaat ttgatgtgac
     5581 tgcgatcgat agtatcaaaa catctttgat gggacctctt gcagggttag gagattcttt
     5641 cttttgggga actttgcgat tgattgccac aggtgtgggt acctctttag ccatgcaagg
     5701 aaatatttta ggccctatct tgttcatttt gattttcaat attccgcaca ttctgtttcg
     5761 ctatctagca acaggttggg gatatcgctt aggaacgggt tttttgaaaa agatccaggc
     5821 aaacggtatg atggaaagtt tgacattagg cgcgtctatt attggactga tggtcgttgg
     5881 agcaatgact gccacgatga tcaccatcaa tattccttta aaaatcggtt caggagaaaa
     5941 tgctgtgact gttcaaagta tttttgatga tatcgttcca aatatcttat gtcttggagc
     6001 atttggcata gtgttttatc tattgaagaa agaagtgaaa ccattaacga tcttgatcgg
     6061 actagctttc tttggaattt ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga
     6121 aaatgtttac gatgcaagat tatatctatg aagaaaagga agtactaagt acgatcttga
     6181 aaaagaatga tttttcgaca agagaacata tgaaaaaaac agtgaattta ctgatcttag
     6241 caacgggttc atcttataac gcttgtttag cagcgaagcc agcattggaa tcttatggtg
     6301 atcttactgt cgatattcaa gaaccttttt attttttcaa ttatggaaaa ctttctcctt
     6361 caatcgatac tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag
     6421 taaaaatgat acaaaaacaa gggttacctg ttgttgctat cacaaatgac gtgcaaagtc
     6481 cacttgcttt agaagctgca caaattattg atttaggtgc cggggtagaa tcagtaggct
     6541 tcgttacaaa aggctattct gcgactgttt tacagttact actcttaggg ataggaattg
     6601 gaataagtaa aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca
     6661 aaataatcaa ccatttacca gcgattattc aaaaaacgga agaatttttt gatggatatc
     6721 aaagtttatt tcgtttagca cagcgtttcg tcaccattgg atatggtcca aactggggaa
     6781 cagctaaaga agcagagacg aaattgactg aaacaatccg cgttccgtca caaggatttg
     6841 agttagaagc ttatatgcac ggcccatacc tggaggcaga tgcttcgcat ttgctattct
     6901 ttattgaagg ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc
     6961 ggtacgttgg agagactctc accattacca caaaaaaagc aagaaacgag aaaacacttg
     7021 gattagcaat agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac
     7081 tattcgctta taaaaccgca gtagctaaag gtattgactt gaacaaaaag atttttgaag
     7141 actttgatac agtcttgaaa agtaaattat aagaggtgga aatcatgctg aaattcaatg
     7201 aagaagaaca aatcaaagca ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa
     7261 tcatcgataa actttatgcg gaaaaatttg acgctgtcta ttatctagga atcggtggaa
     7321 cgtatgcttc ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct
     7381 ttgtacagca tgctgcagaa tattacacta ctggaaacaa aagattaaca aaagattcta
     7441 tcgttatttt gtcctctgtt accggtacaa ctcaagaagt agtgaaagca gtagaacaaa
     7501 tcaagaaagt aggagcgaca ctgattggat ttatcgataa agcgaacagt aagttgtctc
     7561 agctatgcga ttttgtggtc acttatccag ctcctggaac agaacaaatc aaatttttta
     7621 tggcagcaga tcgattgatg tatttgaatg gagaattcga agcttattca gaatactatg
     7681 aacagctcga acgttattta ccaacagggt tagtggaagc agagaaaaaa gcagatgcat
     7741 ttggcttatc atttgcagaa aaacatcgac gtgatagtat gcattatttt attggagcag
     7801 ggaatcaatg gggagctgtt tattcatatg ccatgtgtta ctgggaagaa caaagctggc
     7861 ttccatcgaa atctatccat gctgccgagt ttcttcacgg gacgttagag atcgtagaag
     7921 aaacaacccc agtcacatta tttttaggcg aagatgaaca gagagtgtta tctgaacgtg
     7981 tggctaaact tttgccaaaa atttgcagca attatacatt gatagacaca aaagactatc
     8041 cagtagaagg tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg
     8101 tagtgacaca acgcatcgat gctcatgtgg agcagttgaa ctgccatcca ttagagatac
     8161 gccgctacta tcgtcagttt gattattagg atagactgga taaaaagaat ataaaaaata
     8221 aagagagcag cgataactgc tccctttatt tttttaaaat attggcatca attacgttat
     8281 aaaacacatt ttttgcatta tctacagttc aacttataat ctgtttaatg ggacatgtgg
     8341 gtgccaacca ttttttgtta tgcaatagta ctatttagtt gttgcatgga tccgtagtaa
     8401 aagtccaagt acaacgagtt gcgccagttt acatagcgtt ttgtatccag aatgttgact
     8461 aaaacaaaag ttcctatgtc aaccgtcaac agagacaatc tgtccgttgc aggacctgac
     8521 agatttagaa atgttcaatc tgtctcttat gtattttaaa tcaatactca ctaggagaag
     8581 tttcctaacc tcctaatagt aatgctggta gtaacattgt caaaactgct tcctcttagg
     8641 aacaagcaaa gcataacgag tgtaaaaata aaatgtagac tattgtaaca tatcattgat
     8701 tatttcaagt aaaaacgata aaagaagtaa acaaacactt aaaaacaatg gaaattagtc
     8761 ccaactttaa gaaatgtata ctataatgga aattatcaat tatttatttg gaggaatatc
     8821 catgaaacaa aaaatcgcag ttcttggtcc tggatcttgg ggcactgctt tagcacaaac
     8881 attggcagaa aacgggcatg atgtccgtat ttggggaaat gtacctgaac agattgatga
     8941 aatcaataag tatcatacaa atcagcattt tttacctgat ttgacgatcc cagaatcgat
     9001 tatcggatat aaagaattgg cagaagcagt ggataacgca gatgctattt tatttgttgt
     9061 tccaacaaaa gcgattcgtt cagtcgcaca ggaacttatt ggtaaaatga atactaagcc
     9121 aatcattata catgcaagta aaggactaga acaagatacg cataaacgaa tctcagaagt
     9181 tttggctgaa gaaattccag aagaaaaaag acaggcaatt gttgtattat ctggtcctag
     9241 ccatgctgag gaagtggcag tacatgatat cacaacaatt acagctgcaa gtattgatca
     9301 aaaagcagca gcctatgtac aaaaactgtt catgaatgat tatttcagga tttatacaaa
     9361 taatgatgtg attggcgtag aaactggtgc agcactgaaa aatatcattg caattggtgc
     9421 cggcgctatc catggattag gattcggaga tgatgcaaaa gcagcaatca tgactcgagg
     9481 attggcagaa atcagccgtc ttggtgtcgc gatgggtgcc aatcctctga catttatcgg
     9541 tttgagtggt gtgggggact tgatcgttac ttgtacaagt gttcattctc gcaattggcg
     9601 tgccggtaac ttgttaggac agggacataa gctggaagaa gttctagaaa acatgggaat
     9661 ggtcgttgag ggtgtggcaa cgacaaaagc agcagtagag ctagctcaac agctaggtgt
     9721 tgagatgcca attactcaaa caatctataa tgttttgtat aatggagaag atatcaaaaa
     9781 agcagcaaaa gaaatcatgt tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta
     9841 aatagatgat caatagttaa tc
//
LOCUS       DAYYOA010000101         9731 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000101 DAYYOA010000000
VERSION     DAYYOA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.102"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..195)
                     /locus_tag="ACHBKA_002221"
     CDS             complement(<1..195)
                     /locus_tag="ACHBKA_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445883.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="ACHBKA_002222"
     CDS             complement(211..825)
                     /locus_tag="ACHBKA_002222"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM9445884.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="ACHBKA_002223"
     CDS             complement(972..1655)
                     /locus_tag="ACHBKA_002223"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9445885.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="ACHBKA_002224"
     CDS             complement(1764..2222)
                     /locus_tag="ACHBKA_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM9445886.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="ACHBKA_002225"
     CDS             complement(2306..4069)
                     /locus_tag="ACHBKA_002225"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM9445887.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="ACHBKA_002226"
     CDS             complement(4290..5750)
                     /locus_tag="ACHBKA_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445888.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACHBKA_002227"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACHBKA_002227"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM9445889.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="ACHBKA_002228"
     CDS             complement(6907..8334)
                     /locus_tag="ACHBKA_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9445890.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="ACHBKA_002229"
     CDS             complement(8365..9579)
                     /locus_tag="ACHBKA_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM9445891.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2881 a         2032 c         1618 g         3200 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata a
//
LOCUS       DAYYOA010000102         9601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000102 DAYYOA010000000
VERSION     DAYYOA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.103"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..2105
                     /locus_tag="ACHBKA_002230"
     CDS             177..2105
                     /locus_tag="ACHBKA_002230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9445892.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2243..2698
                     /locus_tag="ACHBKA_002231"
     CDS             2243..2698
                     /locus_tag="ACHBKA_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM9445893.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2778..2906
                     /locus_tag="ACHBKA_002232"
     CDS             2778..2906
                     /locus_tag="ACHBKA_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445894.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3168..3437
                     /locus_tag="ACHBKA_002233"
     CDS             3168..3437
                     /locus_tag="ACHBKA_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445895.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3837..4091
                     /gene="rpsT"
                     /locus_tag="ACHBKA_002234"
     CDS             3837..4091
                     /gene="rpsT"
                     /locus_tag="ACHBKA_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM9445896.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4180..5226)
                     /gene="holA"
                     /locus_tag="ACHBKA_002235"
     CDS             complement(4180..5226)
                     /gene="holA"
                     /locus_tag="ACHBKA_002235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM9445897.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5264..7537)
                     /locus_tag="ACHBKA_002236"
     CDS             complement(5264..7537)
                     /locus_tag="ACHBKA_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM9445898.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7530..8069)
                     /locus_tag="ACHBKA_002237"
     CDS             complement(7530..8069)
                     /locus_tag="ACHBKA_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM9445899.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8205..8423)
                     /locus_tag="ACHBKA_002238"
     CDS             complement(8205..8423)
                     /locus_tag="ACHBKA_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM9445900.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8420..8665)
                     /locus_tag="ACHBKA_002239"
     CDS             complement(8420..8665)
                     /locus_tag="ACHBKA_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9445901.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8781..9509)
                     /locus_tag="ACHBKA_002240"
     CDS             complement(8781..9509)
                     /locus_tag="ACHBKA_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM9445902.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2970 a         1863 c         1665 g         3103 t
ORIGIN      
        1 ttatttgaga aatgttctgg ttttttattt tctttaataa taatcattat aaataataaa
       61 aacaacatca agcttaatca ccaaatgata accattatcg ttctcattta gctgttatta
      121 ttatcaatta catttttatt atgctatcct tgttttgaca aggaggtcta ataacgatga
      181 ctaatcagaa aaaagctttt gcagcaacaa tcggctgcct tttatttatg atcttcggtt
      241 ttatcttcga ccgattctct cttaccatca ccccactttt ttatatcagt gccattatca
      301 tcggtggata taaacaaaca tcggaaggct tgaaagaatt gtggaatgat cggacactga
      361 atgtggattt attgatggct ttagctgcta ttggtgcctg tttgatcggt aattactttg
      421 aaggagcaat gttgacattc atcttttgtt taagcggtgc attagaggaa tacacaacta
      481 ataaaagtca aaaagaaatt actgctttga tgaatcttca accacaaaaa gcccaattat
      541 taaaagaaaa tggacaaatg caagaagtag atgtttctca actaaaggtg gataacttgg
      601 tatttgtcgc taaaggagca gctgttccga tagatggctt tttagaaagc acacaggcaa
      661 cgatcgatga gtctgcatta agcggtgaat ctgtgcctgt agaaaaatat ttaggcgatg
      721 agatgtatgc aggaacgctg aatcaaggaa atcccatcac gattcgagta acaaaaacga
      781 gttccgaaac tgtctttgct aagattatcc aattagtaga agaagcgcag aatacgccaa
      841 ctcaaacagc ttcatttatc gaacgaatcg aaaataatta tgtaaaattg attttgcttg
      901 ctgttcctct aatgatcctt ctcccccact ttttcctagg ttggagttgg gatgaaagct
      961 tttatcgagg aatggtatta ttagttgtgg cttctccttg tgcgcttgtc gcatctgcta
     1021 caccagcttc tctagctgct ctttcaaatt ctgcaaaaaa tgggatttta atcaaaggcg
     1081 gtatccattt agaaaagctt tctgaattaa aagcgattgc ctttgataaa acaggaacgc
     1141 tcactaaagg aaaaccagtt gtaactgaca gctttttctt tgatgaaaag gatttggcac
     1201 aacaacttct tgttgctatg gaacaaaaaa cgacgcatcc acttgctcat gcgattttgc
     1261 aatatttcga ttgcacaata cctgaagaaa tccaatactt accaatagaa gaaattactg
     1321 gttttggatt acaaactacc tatttaggtg cgcaatggaa agttggcaag cacgcatatg
     1381 atcctgagac gatgataatc agcaaagaga ttgcggaaat gatcgaacgt ttggaaaatc
     1441 aaggcaaaac cgtgatttat ctttctaaag atcagcagtt aattgctgtt ttaggtctgc
     1501 tagatattcc aaaagcaaat acccaacaag taatttccta ctttaaatcc caaaacatcc
     1561 atacaagtat gataaccggc gaccattctg gcacagcaaa ggccattgct gaacaagtag
     1621 ggattgaaca ttactatgca agctgtactc cggaagaaaa aacacaacta gtcaaaaaag
     1681 agaatgaact ttaccaagta aatgcgatgg ttggtgatgg agtcaatgat gcgcctgctt
     1741 tagcagccgc ttctttaggt gtcgctatgg ggcaagggac agatgccgct atggatatcg
     1801 cagacatcgt cttaatgaaa agcgacttaa ataaatttgt ctacagtcat cggctatctt
     1861 taaaaatgcg gcggatcatc aaacaaaata ttattttttc gattcttgtt attctcttgt
     1921 tgattgcttc taacttcctg caatttttaa atctacctct tggagttgta ggacacgaag
     1981 gaagcacaat attggttatt ctgaatggct tgcgtttact aagaccttta cctgatcttt
     2041 ctgattcttc aaaaaaatgt aagtcttgtc cactatacaa agcgcggctg tcttctgtca
     2101 aataagcaaa gtgttttttt atgattttgt ttattttatg aaaaaaacag cttatgaata
     2161 ggtcttgaag gatgaattcc tacttttgta actttctaaa aacatgttat gattgtttcg
     2221 aagaaagtga ggaatatatt tcatggaaag ttggcttttt ttaggaatta ttttacttgt
     2281 agcaatttta gctaaaaatc aaagtctgat gatagcggca gcttttgttt tgattatgaa
     2341 gctcatacca gcctcccgtt cctttttccc ttggcttcaa ggcaaaggaa tcaatattgg
     2401 ggtgacgatc atcacagcgg ctattctaat ccctatcgca acaggagaaa tcggtctacg
     2461 tgatctatta gaagccttta aatcccctat tggctgggta gctgtttttt gcggtgggct
     2521 agtggctgtc ttatcttcca agggtgtagg attgattgct tctgatccac aaatcactgt
     2581 tgcacttgtt ttcggcacga ttttaggtgt tgtcttctta agaggaatcg ccgccggacc
     2641 ggtcattgct tctggtatca cttactgtat cttacaagta tttgctttag gaaaatagaa
     2701 caaattttgt ctattttagg agattcttaa cacccccaat ttactgatcc gtgatatact
     2761 gacaacgagg tgatacaatg ttcgaaaaat taaagtattt cataggaaca atcggctggt
     2821 ttcttctagc ggtacttttg attctgggct tagcttatct attatttcct gatttgatac
     2881 aaaatgtgtt tgatactgct ttttgattga cctattaaaa cgatataggg gatgctcctg
     2941 cgatgcaggg gcttttttta atcaatgttt tgtaataatt taggccgtgc caatgagact
     3001 tcctatagaa ttcgatgctc ttttttcttt ttggctcttc tgctcttttt tgttatacta
     3061 gattacagtg gacaaaagaa agaatgatcg catccacttc aacgaatatc aaaatggaag
     3121 aattagtgcc tgttttcagg ttactttgaa aagaaaggaa gacgcttgtg gaaacttttg
     3181 aaggtctttt aattgatacg tacgttttac aagaacagcc aatgatgctg actgggatca
     3241 acttgcagct tgaggaccgt gtgatcaatg gcctgcttaa agatcgctat ttggcaaaat
     3301 atatcttacg tttgccccta aataaatatg tgctggaagt aagtggcaaa tggaaccgtc
     3361 ggaaccagtt ggtcataaaa aaaatgttga taaaaaatat cgatgaatac attaaaataa
     3421 tcggtacacc agaatagcct ttactgatca aaggatactg gttataaaat aactgcagtt
     3481 tcttaataga aaaacaaaga acaacaggga atcgtatccc ttttcaaaaa aaactttgca
     3541 aacggttctc tttgttatat aataacttag taccaaacat tcccattcaa actttgttta
     3601 aatagataaa taaaaatcta gtttggtttg acgctattgc ctcccacaat agcgtctttt
     3661 tgattttttt cagcttgtac tatccgtttt tactgaaaaa aaatcctaat aaaagactgt
     3721 taaggaaaaa aacttgtcat gttcatattg caaagcacgc tgcctttagg tataataatg
     3781 aatgttgaat tatgcggtaa cacgcgtaag aacttaagtt atcggaggtg aaactaatgc
     3841 caaacattga atctgcaatc aaacgtgttc gtacaagcga aaacgctaat gtgaaaaatt
     3901 catctcaaac aagcgctatg cgtactgcaa tcaaaaaatt tgaagatgct gtagcttcag
     3961 gtgctgacaa cgtagatgcg ttatataaag aagctgttaa agctattgat atggctgaat
     4021 caaaaggtct aatccacaaa aacaaagcta accgcgacaa atctcgttta agcaaaaaaa
     4081 tcgctaaata aaagaatcgg cttcggccga tttttttatt tgtcaaaaaa aggacaaaaa
     4141 caaacgacct tcccgcatgc tttgccctcg tctttcttct tatcgatttc ttaacaactg
     4201 ccctgtgcgc aaaacgaata gttgaaaact taattctttt tcgatttgtc ccgttttgat
     4261 ttgataatcc atctcaacta attgatcaaa caactgtacc agcaaatgtt catcgaattt
     4321 ttttacttcc tgcatggcaa gttttacacg atatggatgg attttcaatg tttcagcaat
     4381 atttgcctgc tgataaccaa tctttacaag aaattttgtc tgtaaaaata aacgtaattg
     4441 agaaagcaaa atcgcattaa ttttgattgt ttcttcccct tgcataacta aatcttcgta
     4501 taaacgcaaa gcttgttccg tcttcccttt taaaatatat tgggtcatgt caaaaatatt
     4561 atgttctaac gttttaggaa ctaattcttc tacttctctt accgtgattt gctgttgatt
     4621 ttgtccataa aggaccaatt tctgtaactc tctcatcatt ttggataaat ctaagtcggt
     4681 caacctcaag aataaatcaa ttgcacttcg ttccattttg attgaggaat tctctaaggt
     4741 attcatcaga tattgacgta cttctttttc attcaactgt tggacattga tgataattgc
     4801 tgtttttttc aattgtttcg ttactttttt ccgttcatcc aacttttcat atggcgcaaa
     4861 gaatactaat acagtcgatt ctaatggttc tttcaaataa gccgtcaata aatcagtatt
     4921 ttgttcgatt ccattgttta ctttttcacc tgttaaaaaa taaggattct ctacgaatac
     4981 taaccgttga tctccgaaaa acggcaacgt ctctgcttca gctactacct catctaaact
     5041 tgcattttcc atgtcaaaac ttaaaaaatt taagtcatct tcgccctcta attggatttt
     5101 tttgacaatt tctgtccgaa cttgatcttg gagaaacttt tctgttccta aaatcaagta
     5161 acaagatgca agtttttctt tacgtatttt ttgtaactct gcttgtatac tcaaattact
     5221 caccactttc aaaaattatc ctaccatgag cagatgagaa ttactagtct atcattgttt
     5281 ccactagccc atgaggatga aaaactggat gccagcgata ggtgatcatt ccttgttgat
     5341 ccgttcgcca tgtttttatc tgattttctt ctagtgtctc caatacttcg ctgtgcgggt
     5401 gaccataacg attatttacg ccgcaggata taatcccatg ttttgcttca atatgggaaa
     5461 gaaaatctgt gttacttgac gtacggctac cgtgatgacc taatttgact acatcggctt
     5521 tcagattagg ataaatcaaa agcaattgtt tttccattgc ttgattcaaa tctcctaaaa
     5581 ataaaaagcg cctattgtta tattccatcc atagaccaat cgaatcttca tttcttccct
     5641 ctcccttttc aaaaggagtc aatacataaa gatcataata accgctgatc ctgtccccat
     5701 gtttgatttc aatgatctca gtaccatttt tttgagcttc agataaatgc ctttgtaatg
     5761 gctcatttcg tccagcgccc caaccgatat aaattttttt gatagtaaaa tgctgagata
     5821 acgtgggtaa ttctcccaca tgatccgtat cgtcgtgtgt caagatgagt ttatcgatta
     5881 cacttatccc ctgacctttt agatatggaa cgatatttct ttcagcagga ggacgtatca
     5941 taccttgcgc ccacttttct ctttcaaagt gtaactgacc tccagtatcg atcaaaatcg
     6001 tttcttttcg aaaaggactt tgcaaaaaga tactgtctcc ttgaccgaca tcgataaaag
     6061 cgatcgttga agaaaagcgt aaaaatggca aagtcaaaaa aataaaagaa aaaagaccaa
     6121 cataaaacaa gtgtttcaca ggttgttctt tcaacctatc catacaaatc agtaatccga
     6181 aaatatacat cccagatagc acaaggggca aacgtcctat aatgagagac gaccctgaag
     6241 aaaaatcgat tattcgctca aataagatta atccctcttc tatcagcaaa agtaaagttt
     6301 ccggcagcca gcgattgaag agtgtgagaa ataacacacc aggaagaaaa agaaacttga
     6361 ataccggtgc aatcagtaat gtaaaaagac tacctaaaat aggccactca taaaaatgcc
     6421 atacaatcaa cggcatcgtg atcatagtaa acaggacgtg agaggccgtt tttttcaacc
     6481 aatctccatc aaaagaaatc aggataaaca aaaaactcat gaaaaaacta agctgtccac
     6541 ccgcatgaaa aagaaatcga ggatcaaata gtaaaagaag ccaaaagaca actgcaaata
     6601 agtccatggt ggaataatgc ttattcagca atttgactag caatcgcaat aaaaacagca
     6661 aactagcacg caaaacagaa atagaacttc ctgcaataga aacataacag atagttagta
     6721 gactcaacgg aataatactg acgtgtagtg gccatccgat acgtcgaaat aaataataca
     6781 gccaccctaa ataaaattgg atgtgtagac ccgatagact gaacagatgc agcaaaccac
     6841 tctccctaaa aatttcttct ggttctctga atcctgcatc tttatagccc cataataaag
     6901 aattaatata tacacttgtt ttttgcggaa attcagattg gacaaaggag ataaaagatc
     6961 ctctttttct ctgaagccag tttcgccagt gattttgcat ggtgatctgt tcaatctttt
     7021 gaatttggaa ccgtccagaa attctatggt tcctgaagta agcttcttgg tcaaaattat
     7081 aaagattccg cttttttgct ggagagatga actcgccttc agcagtcaat tctattccct
     7141 ttcctcggaa gttaagccat gttttcaatt ctgcttctgt gtttacttga taagaaagct
     7201 ggagcttttg tttattctct gataaccttc cttccattgt caaaaaatta ccatttgcac
     7261 gaatcgtatc tgtaaataca gtaatttgcg cagatgttgt tttttcctct aaaaatgaat
     7321 actcttgtag cctataagaa atggataccg ttatgctaca caaaatacac aagtacgcga
     7381 taaaacgtcc ttcttttagc gaaaaccata aaaaaattcc ggcaagaagc aaacgataac
     7441 cccaattgtc agtgtaatac aacaaacaga taatcatcga cgtaattgcc ggaaaaatca
     7501 accgatttcg aacatgcctt tctaaaacgc tagacatgtt ttcctgttaa ttcattttga
     7561 agttttgaaa aatgctgtga gtctaaagca acttggtgta cagatacacc taattgttcg
     7621 atcaacgctt gggcataagg atcatttcga tagtcatgga gataattgat ccctttcaca
     7681 ccagcttgca acaaagcctt tgtacaggcc aaacatggaa aatgtgtgac atagatttct
     7741 gaaccttccg tcgggatacc aagttttgca cactgtagga gtgcattcat ctctgcatgg
     7801 atcgttcgca agcagtgtcc atccacgacg taacaccctt catctatgca gtggacatct
     7861 ccactgactg ctccattata gccgccagca atgatccttt tttctcgaac gagtgtcgct
     7921 cctacttcta aacgtgtaca cgtactacgt aaagataaca gtactgcctg agccataaag
     7981 tactggtccc aaggaatgcg ttcattatgt gacttatcag taactttttc tttattcgta
     8041 tcgcatgctt ttgaagattt ttctaacatg ttttatattc ctccgttttt tcaagttgat
     8101 taaaaagtac tcattgctta aacaaaatat tctttaaata taacgaatca tcctgtctga
     8161 caaaagatcc tacccttcac tgccgaaatg aagtccgtca tccattagcc cattataaaa
     8221 attcttcgaa tccatttgtt caagaaattc cgtataaaaa tcgatacttc acaatcatgg
     8281 cgttttgcta cttctgtgag aacagagata tattctttga tcaacttgtt attccgatat
     8341 ttttgttttg tctcatcgac tgctggtggc gtgataaaga aagcatgatc agaactatag
     8401 tcttttttca gctgaactat catccactgc agattttttg gtacgtttct ttagaaagtt
     8461 gtttatgctc tgctagatca ttcgtcccaa tgagaataaa tactttatct gctgtatagc
     8521 taagtacatc tttggcaaag cgttctcgta gagcttgact gttattgccg ctgacagccg
     8581 tattcataat atgaatccca gatattttat tttggagata gacattcagc attggttttt
     8641 ttaatgtttc tttgcgaaca atcaagctat ttcctatcca aaaatactac ctcctcgtcc
     8701 tgccaagctc ttagaattgg attattctta gtatccatta gcgtccattt attgctcgtg
     8761 ccagtcgatt ttgaatcatt ctatttaatt gtaacgaagt tttttagttt ttcaactgtc
     8821 ttttcaccga taccagaaat ctcctgcagg tcttccactt tctggaagga tccgttttct
     8881 tctctaaatc gaatgatttc ttgcgctttg actgatccga tcccaggaat ctgctgaagt
     8941 tctgatggag tggctgtatt gatatttatt ttgggaggct cggaatctgc tgtaggctgt
     9001 tttccttccg aggatgccaa ctgatcaaga tgttctggca gctcttcacc tacagcaggg
     9061 atatatagga cttgctgatc ttctatcttc gcagaaaagt tgattcgatc gctatccgct
     9121 tcttctaata atccacctgc acgctttagt acatcataga cccgttcttc tgaagaaaag
     9181 gaatagatac caggctcctt cacacttcct ttgatatcgg tgtacatgac ctttggacga
     9241 gcatcctctt ctacatcgtt tccttcagct gatggatctt ctccactgga actaaacgaa
     9301 gaaagtattt cttttgcagc agtctcttct gtttctctat aaaatagcaa atttgataat
     9361 aaaaaaccac ttgcgataca aaagataaga actatcatag acatccaaat gatcaatttg
     9421 ggtgaagcta gtattttctt gctgattttt tctatcattg ttcttttttc aaagaatgac
     9481 cagtaaaaat cattcattac tcctttcatt taaaattgaa gaaacagtga gctctggcaa
     9541 aatcgtccct tcaacatcta attacgtcaa ttttttcttt tgtttttatt tttatcgtga
     9601 a
//
LOCUS       DAYYOA010000103         9546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000103 DAYYOA010000000
VERSION     DAYYOA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.104"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACHBKA_002241"
     CDS             <1..152
                     /locus_tag="ACHBKA_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445903.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACHBKA_002242"
     CDS             complement(230..763)
                     /locus_tag="ACHBKA_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM9445904.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHBKA_002243"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHBKA_002243"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM9445905.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHBKA_002244"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHBKA_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM9445906.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHBKA_002245"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHBKA_002245"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9445907.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACHBKA_002246"
     CDS             complement(4488..5066)
                     /locus_tag="ACHBKA_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM9445908.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ACHBKA_002247"
     CDS             5388..5858
                     /locus_tag="ACHBKA_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM9445909.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ACHBKA_002248"
     CDS             complement(5966..7192)
                     /locus_tag="ACHBKA_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9445910.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ACHBKA_002249"
     CDS             complement(7946..8146)
                     /locus_tag="ACHBKA_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9445911.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ACHBKA_002250"
     CDS             complement(8280..8888)
                     /locus_tag="ACHBKA_002250"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM9445912.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ACHBKA_002251"
     CDS             complement(8973..9443)
                     /locus_tag="ACHBKA_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM9445913.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         2832 a         1912 c         1567 g         3235 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttg
//
LOCUS       DAYYOA010000104         9456 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000104 DAYYOA010000000
VERSION     DAYYOA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.105"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..283
                     /locus_tag="ACHBKA_002252"
     CDS             89..283
                     /locus_tag="ACHBKA_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445914.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            552..851
                     /locus_tag="ACHBKA_002253"
     CDS             552..851
                     /locus_tag="ACHBKA_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445915.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(948..1382)
                     /locus_tag="ACHBKA_002254"
     CDS             complement(948..1382)
                     /locus_tag="ACHBKA_002254"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445916.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1614..2324)
                     /locus_tag="ACHBKA_002255"
     CDS             complement(1614..2324)
                     /locus_tag="ACHBKA_002255"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9445917.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2314..3174)
                     /locus_tag="ACHBKA_002256"
     CDS             complement(2314..3174)
                     /locus_tag="ACHBKA_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM9445918.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3179..3817)
                     /locus_tag="ACHBKA_002257"
     CDS             complement(3179..3817)
                     /locus_tag="ACHBKA_002257"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM9445919.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3832..4131)
                     /locus_tag="ACHBKA_002258"
     CDS             complement(3832..4131)
                     /locus_tag="ACHBKA_002258"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9445920.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4174..5658)
                     /locus_tag="ACHBKA_002259"
     CDS             complement(4174..5658)
                     /locus_tag="ACHBKA_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9445921.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5678..6139)
                     /locus_tag="ACHBKA_002260"
     CDS             complement(5678..6139)
                     /locus_tag="ACHBKA_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9445922.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="ACHBKA_002261"
     CDS             complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="ACHBKA_002261"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM9445923.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7442..8212
                     /locus_tag="ACHBKA_002262"
     CDS             7442..8212
                     /locus_tag="ACHBKA_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9445924.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8485..9312)
                     /locus_tag="ACHBKA_002263"
     CDS             complement(8485..9312)
                     /locus_tag="ACHBKA_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9445925.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2947 a         1938 c         1482 g         3089 t
ORIGIN      
        1 tatttatcat aaagatatag atacataatc attttcactt acatatattt gaatattaac
       61 taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa caaaaggcgt
      121 caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg gttcatggaa
      181 agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc tagaaaaaga
      241 actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat gtatagaaag
      301 gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta gaagaagaag
      361 acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt atttcaatat
      421 gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata tacttgtcat
      481 tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc agtatagaaa
      541 ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag caatttccac
      601 aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag tatttggagc
      661 ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata tacgagatga
      721 aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa aatcatgtgt
      781 tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag gtgagtaaaa
      841 aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat cctcttgttt
      901 ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta tttgtcatta
      961 ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc catttccttt
     1021 acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc atctgtcaat
     1081 aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt caaaagcact
     1141 ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat aaatgcgatc
     1201 gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc attacgcttt
     1261 atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt tgtgtaattc
     1321 gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga atcaatattc
     1381 atttttttct tcctttctta tacaattctc cctctaataa tacagttctt tgactattta
     1441 atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt cctctttcct
     1501 tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt cacaacctct
     1561 gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca tctctattgc
     1621 ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa atattgcggg
     1681 atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca aatttcatct
     1741 aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa cggtccatgt
     1801 ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt aaacgtctcg
     1861 acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt cgttaattgg
     1921 cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata agctggcaaa
     1981 tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt aatagcagtt
     2041 atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga tggttttaag
     2101 ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc tgcttgcatg
     2161 cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg gtccacttca
     2221 ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag atttgcttca
     2281 aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc cagcctcctt
     2341 caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt cttggctttt
     2401 ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca aatgtcttaa
     2461 taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact ggcctgaata
     2521 ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat cgatcccttt
     2581 ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta ggtctggata
     2641 tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg aaatggagtt
     2701 cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag aagcttcctt
     2761 tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt tctcttcata
     2821 ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg ccaaatggat
     2881 tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag aaccaaacgg
     2941 aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc tgaacatctc
     3001 ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc gctcatccgt
     3061 ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg aaaaacgttc
     3121 tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag ccatttcctt
     3181 acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc ggatcatttg
     3241 ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca aataagtgta
     3301 atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca atcagttttt
     3361 tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca tcacggcttt
     3421 gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt tcaaaagtcc
     3481 aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc tccatagttg
     3541 gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca acattactcg
     3601 ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta tctgggaaca
     3661 tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt gtgcccactt
     3721 caataatatc tactacatca ccaactttca taacatctcc taatgcatgt tctaagtcat
     3781 tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc tttacttgtt
     3841 tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg acatcacatt
     3901 tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat tcaaaaaatt
     3961 gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg aaatggcaca
     4021 atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca cacttctcat
     4081 catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca tcttaatccc
     4141 tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt ctgcatatgt
     4201 gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt gtaactgcgg
     4261 aatagccaat aaaccaatga caactacggc aattccagca taacctagga ctttcatgat
     4321 tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac caccgtatgt
     4381 tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga ttcctgaaag
     4441 aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa ctgctataac
     4501 ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg atttcaataa
     4561 aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc caatcgttac
     4621 cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa cagctcctgg
     4681 taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga atgtccgaac
     4741 acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca taatgtagaa
     4801 taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt tgcctagtac
     4861 aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca tattctcatt
     4921 gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt gcttgattga
     4981 tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa atataaagat
     5041 tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt cctgcgtgat
     5101 tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc ccataaaaat
     5161 caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa aagcggtagc
     5221 tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg tatatttctg
     5281 aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca tcacatctcc
     5341 aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc caaaataagg
     5401 atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga ttggccgaaa
     5461 attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg ttgcctttaa
     5521 gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta ataacacgat
     5581 aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg tccaaatgct
     5641 taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct gccatcacaa
     5701 cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca tctgtcatta
     5761 ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt gctgcgagtg
     5821 taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa cggattggct
     5881 gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt tctgaagaat
     5941 gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct acacaatgga
     6001 tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca agacagcttt
     6061 cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc aattgatggt
     6121 caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg gaaacggtgt
     6181 atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact caagatcatc
     6241 tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt taaatccata
     6301 cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat cagccataaa
     6361 attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg actccgccat
     6421 gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt caccataggc
     6481 ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat tggagtaatg
     6541 tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt ttactgcaat
     6601 ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt cacaagtgac
     6661 caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga ttttgactgc
     6721 atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt gccacgtgcg
     6781 aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa ctgctgcggc
     6841 tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt ctacattttt
     6901 tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca tattttgaac
     6961 gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat gactacgttt
     7021 tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt tcagccaaat
     7081 tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt ctgtttcgat
     7141 ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact tttcttttgt
     7201 tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt ccaacttaca
     7261 agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg tttgtttttt
     7321 atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag atatgaatgt
     7381 ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg aggcaaaagt
     7441 catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg aactgcttca
     7501 gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat ctattagtac
     7561 gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca attacggtta
     7621 ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg aaaggattaa
     7681 acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc tgtttatcaa
     7741 ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt tgacagtcat
     7801 tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt atattttaac
     7861 tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat taaatacgat
     7921 ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg tagtacatgg
     7981 ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca acaagacaat
     8041 caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa agttcaagat
     8101 tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg aaaaagtatt
     8161 agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt aagaaccagg
     8221 acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg ataaatgaaa
     8281 aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa tcgataccat
     8341 cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat caaacaatat
     8401 cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg gtattcaaat
     8461 cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct ttaccgcgta
     8521 atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta aataatcatc
     8581 ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca atgcataagt
     8641 cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg ctttatcata
     8701 atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga ccatcagatt
     8761 atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt tcgcaaaatc
     8821 taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta agtgatggat
     8881 atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg catctggttt
     8941 tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta aaagatcaga
     9001 attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt ggtattttga
     9061 tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt cttctgtttc
     9121 tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta agatattgat
     9181 gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac aaatgccttc
     9241 tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta atgctccatc
     9301 tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta tatcatacac
     9361 aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt aattttaaaa
     9421 taatattcaa attaaacaac tggcgtatct gctcct
//
LOCUS       DAYYOA010000105         9424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000105 DAYYOA010000000
VERSION     DAYYOA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.106"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..459)
                     /locus_tag="ACHBKA_002264"
     CDS             complement(37..459)
                     /locus_tag="ACHBKA_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9445926.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(449..2623)
                     /locus_tag="ACHBKA_002265"
     CDS             complement(449..2623)
                     /locus_tag="ACHBKA_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFM9445927.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2639..4981)
                     /locus_tag="ACHBKA_002266"
     CDS             complement(2639..4981)
                     /locus_tag="ACHBKA_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9445928.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5003..7546)
                     /locus_tag="ACHBKA_002267"
     CDS             complement(5003..7546)
                     /locus_tag="ACHBKA_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9445929.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7556..7954)
                     /locus_tag="ACHBKA_002268"
     CDS             complement(7556..7954)
                     /locus_tag="ACHBKA_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM9445930.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8035..8319)
                     /locus_tag="ACHBKA_002269"
     CDS             complement(8035..8319)
                     /locus_tag="ACHBKA_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445931.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8343..9341)
                     /locus_tag="ACHBKA_002270"
     CDS             complement(8343..9341)
                     /locus_tag="ACHBKA_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9445932.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2681 a         2013 c         1470 g         3260 t
ORIGIN      
        1 ttttgttttt ttcaacgtat tctctttcaa ttttcactaa aaattaggaa gaaattgatt
       61 caatgtcttt tcaaaaaaga atgtttttct ctcaattttt ttgacgtttc tatcatgaat
      121 cacgagaaaa cttgggacac ttttaacatg gtaacgttgt aaaaaagttt gaagttcttc
      181 ttgtcttggt tcttttcttg tttcaattcg atatacattc ctagagaatt ttttgtcgta
      241 tctttccaag aaagattcaa cttcttggca ttttttacaa tcatttcttg ctagatacac
      301 aaaaaatgat tgattcgttt tttgaagttc tagtacttct tctaaattac tattgattag
      361 ttttccattt gattcttcgt tagtttttat cagtttaaaa caaccaaata agaggaaact
      421 aacaataagt atcctaacaa taactttctt atttcgcata ttgtttctcc aaatttggtt
      481 tccgaatata gacgactcgc ggtggttcct ggacgtcttc agctgttcga tacaccacaa
      541 attcgcctgt tctcaatgct cgaatgcgat tcggattgaa tttaaagcgg tctacgtctc
      601 gtttcgtgcc tctttctcct gtccaatcaa cctttcccat atcagaataa cctgcttctt
      661 gtgtcgtcat ttccgttaat tcaatgtctt cttctgtccc cattaaatct gcccaataat
      721 caatttctgt cgggtcattt gtcttcccaa taaagaacgt attcacattc ccaatcaact
      781 gttttcgaag gacaggatca attctatcaa tgtccgcaag cgtttgaggg gcatacgccg
      841 catgcacccc agcgcctcgt cccttattga ctaagtctat aaggttatca tttagataag
      901 aagctggttc gtcaaatatt actaggactt tcgagcgatt ggctgtttct cctgcttgtt
      961 tatacacttc tgacgcaaag aaattaatat ctcccacaag catttgagcc aatgtccgaa
     1021 taaactcact ataaatcaat ccgtttaacg agatataaac aatctttcct tctttaacaa
     1081 cttcttgaat atctaattct ctttttcctc cagaagtatc aaagagttca ccaagctcac
     1141 tatccaataa catattgata ttggtctgta aattctcaga agtcgcatac aaataaaacg
     1201 ggttcttctt atgttcgtaa cgaacaaaca accgtttaaa caacgtttgc tcgatttctg
     1261 tcatgagaga aatgttttct tttaataata gataatacgt ttctaagtca acggttttac
     1321 gatcatgttc aacgattcgc tcttcactta actgttccac atcggcaaaa tcacgttctg
     1381 ttttgtccat cgcttcattc tttttcgttt ttttcttcgt ggttttcttc ttttctacga
     1441 ccactttttc tttaaataaa tctggatttt tcaactgaat acgatccgca aacaaatgta
     1501 agacgtttcg tggcaataaa taatgttgta accaaactag atttctttca attttatggc
     1561 tcttttggaa ctcgtctaaa agctgaatcg ttacctgtaa taacgctttg gctgctgtcg
     1621 aataatacac gctttctgtt tctgctagat tggttaattt atctcgaatg gcaatatcat
     1681 tgccattttt tacaggatta taacgcaaat cgccaccgtc cacaaagact tcaatcgggc
     1741 gattgtatgc ctctgccact tcttttaacg cttgtaaagt ctctcctgct cctttcccat
     1801 cgactaagat aacggggata cgaaacttac acgcatgttc tgctagtaat tggatcaccg
     1861 tcgtttttcc tgaacctgta gtcgctggaa taatcacatg ttggttcaat tcactaaagt
     1921 tcatcacata agggagccca tactcatctg ttcctaatag aatcactttg gctaatgaag
     1981 ccagttgttt attccgcaaa gcaacgtctt ttatgacaat tgctacctgg tacgcttgct
     2041 gatactctgc tgtatatttt tgggactcct gaaatttatt cttccgacgt tccttgtatt
     2101 ctttactatt tagcaaagct gtttgccgtt ctttctttga aacaactttt cttttttcaa
     2161 taaaagacgc atacgcaatc aaacaacttc ctgcacctgt cccacctaaa aggagggcaa
     2221 aaatcgtttg tccattaaac tgcaacgcat gagaaaacaa gtttaatgga aataataagt
     2281 aacacgacaa ataccctcta ggaagaacaa aaataaaaca aaatagattt aatagcgtaa
     2341 aacctaacgc aaaaataaaa aaaagagagc ttcttcgttt taagaagctc tcatactcaa
     2401 agtcataaat atgtgcacgt gtctcttttt tatctatcat aaagacaatc gctaaaggaa
     2461 tactgcctac aataaaaaat ggatatagac aaacacctaa taaagtgacg ataaaataaa
     2521 gttgttgaaa ccgacttttt ggcgtttctt tttcttgttt tcttttgcga tcgacagaaa
     2581 acatttgctc aaaatgtgtc ggtcgttttg gtgcattcat catactatcg atttcctcct
     2641 atcgttttcg ttgtttcatt tctcttcgat aaatcggttg tttttctcgt tttcgtttca
     2701 tttttgcttg agttgctcga aactcactcc gtgtcttcgg tgcttgtttt ggtgattttg
     2761 tttgtttcat ggttgagtat gctttagata cggttgaagg cgctttggtt tgtgctgtta
     2821 ctcgattgac tgctgacata tgttcccgaa ccccctctgg acgtcgaaca gatttcggtg
     2881 tggtagaacc tgttgctcgt ggtacattcc cttttcttag tggtttttgt accgcatgtt
     2941 tttgtcctgc aactgtccgg gttttcatcg aacttgtttt tgttacggga gtaagtgcct
     3001 ccttccgacg acgacttgtt tgtccctgac gacgttttcg atcgttcaat tctgttctag
     3061 gcgtatgctt ctggccataa gacttgtgtt tttgctttgg attcgctaat gacaccacca
     3121 ttgcagaacc ctgattgcga tacggactac gagacgcacc ttttctactt ctttctctta
     3181 gactaggatt gccttgcaat tctcgttgta ataaatcggc tgtttttctt gcctgtttct
     3241 ccattgctgt tcgttgcata cgttcttgac tttgctcttc tgcttgtttc gcttgatcat
     3301 gcaagtctct tgctttttgt gccatacgtt ccatacgatt tggtgcttta tgacgaggtt
     3361 gttgtgcgga ttcacgccga aaagcacttc gtgtggcttg tttcgccgtt tgtggcatac
     3421 gaacgtctga gccaccccca cgttttcgtt gacgttcttg tctcttccgt gcgttttctt
     3481 tttctttttg tttctgtgct tttctcgctt ctttgcgttc tttcaaccgt tgtttggctt
     3541 cccttctcaa ttttgcttct gccccaaatg gatttcccgc aaaagctaac gctcctcgaa
     3601 cacccattct tccagtaaat agactgccta aaaagcctcg gaacaaataa attcccaacg
     3661 gcgcaaacaa ataaacaatg atcaagagaa ttttttccca tggatcgtct tgtgtactac
     3721 ttaattggaa cccgtaacta agaaaagaag aaaaaaggat acatgcaaaa cctgtcattc
     3781 cttttaaaaa cgtcaaaata ccaaaaactt tggcatagtt accaaacaca ttcattgtga
     3841 caagaaacaa tcctaaaaaa agtaagaacg gcaacatcgg tactaacaat agttgcatag
     3901 ttccaataat caatcgaaac atacttaaga caaaataaac aacggtttga atcagattca
     3961 cgacaaaata aaacaaggca aatgctgtat ttttccaact attgtaatat tgtatgttct
     4021 tgtttttcag tgtatctgct tcatactctg tgactttgtc atgaattttc tcccctattt
     4081 ttgtacctaa atcattgtcc aataaaagat tgattcgatc atattctttt ccagcatatt
     4141 tgactttctt ctcacgaatt gtgtctacat tggttgtgcc ataatttaac aacagatatg
     4201 gttcataaac attactataa aagacccgag ccgccatcaa tttgcctgcc gtttccactt
     4261 ttttatccgc tgttacctct tctgtttctt ttgtttcata atttgtttct tcttcaggtg
     4321 ttaacactgg attgaccttc acgaacgccg tttccactgt agaatctaaa gaaaaaagaa
     4381 catcaaaaag attcttattt gtcccagaat cttttaatac gcttaaccca gtaaaaataa
     4441 ggatcgtcat aagaaagaca cggagtgctt gtttcattcg agcctctcca gcatatttga
     4501 ttgccatcat aattgccgaa aaactgattc caatagttcc tgcaatactt aacatattgt
     4561 ccgcaatttt ttgtgttaag ttttgaatgg gttctcgaat ggcggaaata atatctaatt
     4621 caaagagaaa ttcaaccatt gaagcattga attctcctaa catttttacg ccactccaaa
     4681 caaaattttt catcactcct gcaacattgt acgatagctt ttttttaagg ttcattgact
     4741 catagtcatt ggtcatcaat tcaaacgaat tctttttgta atttttatac aattgatcca
     4801 catcagtcgt tacttctttt acttctactt tatcgttggc attctctcct tcagaacgat
     4861 cggtttcagt gacacccgca ccatcattca tttgatcggc agaaacagta gccccttgta
     4921 gacagaaaaa aagaccccct aataaaagga gcgtccacca tttcagagat ttttttcgca
     4981 aaaaaaccac ctcttttttg ttttatcgtt gtttttcatt ttctaacatt gcttgaattt
     5041 tttcatcttg attttcagta gaagaaacag ccaataacca actatcaaac aacacatcaa
     5101 tggcaatcgg ttgatttctt cccaaatgat cctggaataa acaggttcct gtttgtaatt
     5161 gttccatcat ttctaaattg gctgaattca cttccatatc aaagaaggac aatagtttct
     5221 ctcgtgcttc tttttgtttc ggacgaaacg caaatttgta agaaattaac tcttttttat
     5281 cttcttgatc gtaatcttta aacgcttgca caacaaggta aatatccgta ttattcgcac
     5341 gtccttttcg caagctatca tcaatcaaat agcttccttg tgcggtatct tcataccctt
     5401 ttgcctcgtc ataaataatc gcagcgtctt cttctttgtt tgtagaaaag atatacgtaa
     5461 acttcataat gacttccata attgccattc ctgcaagttg ttcgctattt aatcgacccg
     5521 aatcaatctc tttttgtgtc ggaatgcgta ttccttgtgt ccctaacacg ttgatttgtg
     5581 aatcaaaacg tatcggcgta ctgtgctcat tcccaatgag aatctttcct aaaccaacgc
     5641 tatgccctaa aatcaattta gctagttctt tgtcactttt tcgaatttct tcgataactt
     5701 gacttaacga atgcttacct ggcatggtgt aaactacatc gatggcgtct tggatcaccg
     5761 ttttcttgtg gctcgctgtt tctcgattgg tatctacttc tcctaacatt cctaacacat
     5821 ttttagcggt ttgaattgcc tcttcttttg gtaaaaacaa tagaggatca agcatccctc
     5881 gacagctttc atctgaagat aacgaaataa aattaattcg ttcatacata gccacaaact
     5941 ctggtatgtg tccgaatttt tcaagtgcct ttaaaaagaa cttttgggtt tcatttttcg
     6001 gatcaatata aagaattttt tgacctaaaa acatcagcca taaaaaaata tattttacaa
     6061 ggaccgattt tcctttacct ggtggaccaa taatcaacgt atttccattg gtatgttccg
     6121 cgccttcaat acttttcttt gttaaattgg gataatacca tactagttta ctagatagcg
     6181 ctaacgcttc tttcgcagat ttaaactttt ttgcaataac aatccgtcct aaaggaaatc
     6241 ctttcttatt tcctacttct ttctttaagt ccaagccata atctgccaca aaacctgtcg
     6301 tagcgacctg ctcgtaacta cgaaaacgtt gcgtacttcc aattaagcat tgattaaaca
     6361 acgttagctg atccgctacc gatcgataaa atttgaaatc tgtagactca atcgcaaatt
     6421 caatatcttt gactcgttct tccaattctt cttttgaaga agcagataca accagcgtga
     6481 cggtagaata acacaatcgt ttttctttgc gcttcagctt atcttttaca tccattaacc
     6541 gttctgtacc aaaatttaat acatcgtctg aatctaaaat gccgtcaacg gtctcatatt
     6601 ctttttcttg ttcataaatc cgtccagcca ttttattaat ttttcgttca tcagattttg
     6661 aatgctcaaa tcgaagtcga atatgcgtat caattggaaa agataagcta tcctgaagat
     6721 tttgaataaa gctactccca tacatggcta aaggagcgtc cgtttgactt aaaaacgtcg
     6781 aataatgcgt ttgattcaac tgttcaatcg caatgtatcc tgcttcattc gaaatgacgc
     6841 cctcgcaaat ttccattggc gtaagctgtc tttcaggcaa tttttcttct gatcgatgga
     6901 aaaaataata ataaatttgt ccaatctctt gggcagataa gcgctctatc ggcttataat
     6961 tggacaattc cgtatagagc tccttttcta atgtttcata cgttggtaac acactactta
     7021 taggaactcg ttgtcccgta atacgataga taaacttttt aaaaacatgc ttaagcaatt
     7081 gaaaatattc gatcggatca gacactttca tttctgtagt gaaacgcaca aacagatacg
     7141 ttcgatactc gtttaattgc acttcgtctt ttaaaatctt tcctgcctgt tcaaagtaca
     7201 ctttcccaag atctgcgcat tctcccttta ccaaatctcg aatggtttct tcaatgtgcg
     7261 tatctaggtt aaattgacgt ggaatattga taaaatgata gtcatattca tcatgtccaa
     7321 gtatgccttc gccatcttga agatagttct tgaaaaaatc aatgtcattt aaaggaaata
     7381 cttgggaaga aagttgatag cctacccaca catcatcatt tttggtcaaa acaaaatttc
     7441 ggataatggc gtctatcgga aattctaatc gaggaagctt cgtttttttc tttttgaact
     7501 gccaaatccc gataataaag acgaccaaaa gaaacaaaaa cgccatttac tcgactcact
     7561 ccttccattt gtgtagggaa tacgtcactt tttcatggaa aaacggcaca tacatttcat
     7621 gttcaaagcg ttgtttccgt ttctgataat aggacgttcg cccaattaaa tactcgaaaa
     7681 agtgtttatt gtcccatttc attgaaaaca atgtccaaac gagaacacca atcccaaaaa
     7741 tcataatcaa ccaggacttt cgaaacagtg tctgcaagaa actaatttga ttcacatacg
     7801 caatcaaaaa taggatcaaa agaatgaaga gaaatacccc acctatgact aattgttcca
     7861 tgcgaatgcc attagaaatc cagggaattt tttgtccttt aatcccgtaa attttgtgag
     7921 ggtgcttaaa ctcctttgta tagttgtatt ccatgttcac tctacctcct tttacaaaaa
     7981 aagaagcgac cagaagttct aatcttctga tcgcttttgc agtttgtctt tttcttatga
     8041 taattgtatc cagggcacaa tcatttgcgt tacccccacg ttctcttcac tatccttaat
     8101 aaagataatg ttctttagcc attcaccaat atcaacggca ttcgataaca ataagacgag
     8161 cattatcaaa ataaagacgc caataatggc aaacgctgcc cgcgcaaagc cttgggtcgc
     8221 ataagcacca accattagaa caatcgcaac gattgccaat agccaaatga cttgtccaga
     8281 tacatcatca aataacgatt taagatcaaa aagtttcatt tccattctcc tctaagttca
     8341 ttttattcat ttaacgtagt aacaaaataa ctatcgccac ttgtttttac ttccattgaa
     8401 aagtttgatt ggaactcgtt tcctttctca aaatagaaac gatacttccc ttgcaaaaag
     8461 tagtttcctt ctttattttt tactgcatga tccagttgta cattgatcaa ttcgccgcct
     8521 tctaaccctt tttcgttaga aatcaatgcc aatttttcat catttttgac atataactca
     8581 aaaaaccgtt tcacaaatgc ttccagcttg atttcttcgc tttcagtgac cgcttgccca
     8641 gaagaataaa atcgattttc ctgatagttc acagaattgg tattcttact ctcagaaaga
     8701 ttcaaaaatt gaacgccatc aagtactttt aaaacattgt tttgataact gactggaaaa
     8761 gaaagttgac tgcttttcca atgttctcct tctgtaaaag taacctcata aaataggcga
     8821 tacattgctt gtgtcaaatc gatttcttct agtttaataa agcgaacatc tttcgctact
     8881 cgattggtag ccgtcgttac aaagcctaga tcagacgctt ccaatccttg tgctaaataa
     8941 tgattgacgg aagcctccca ttcgtttcgt ttttctggcg aataagtaaa taacctttca
     9001 ataaaatgtt gtccctcgta cttcaattgt tccgtagaag acaaggtttg gttgacttca
     9061 gttaacaatg tttctggatt gacttgtttt ttcactgcta aacgcgttaa tgtatccact
     9121 cgtccaaagt ttaggaggag cacgacaccc attaacagaa acaaacaaaa aaaagcgcct
     9181 gaaaatacac tcatggcact tttttgcgaa aattggatca ttcgatctga cggttctgtt
     9241 ttcttttttt ggaacaaaaa acgtttcttt tgtttctttt ccttctgttg tttcggtggc
     9301 ttagtttgga acgcttgtgg attaaatttt gcattactca tgagaaatgc cataccgttc
     9361 taacaataat ttcggtattt tgtccacaat cactacttcg tggacaacca cacttgcccc
     9421 ctcc
//
LOCUS       DAYYOA010000106         9361 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000106 DAYYOA010000000
VERSION     DAYYOA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.107"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..760)
                     /locus_tag="ACHBKA_002271"
     CDS             complement(92..760)
                     /locus_tag="ACHBKA_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9445933.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="ACHBKA_002272"
     CDS             894..1181
                     /locus_tag="ACHBKA_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445934.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="ACHBKA_002273"
     CDS             1597..3135
                     /locus_tag="ACHBKA_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM9445935.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="ACHBKA_002274"
     CDS             3132..4097
                     /locus_tag="ACHBKA_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9445936.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="ACHBKA_002275"
     CDS             4084..5406
                     /locus_tag="ACHBKA_002275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9445937.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="ACHBKA_002276"
     CDS             5647..6390
                     /locus_tag="ACHBKA_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9445938.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="ACHBKA_002277"
     CDS             6405..7397
                     /locus_tag="ACHBKA_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9445939.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="ACHBKA_002278"
     CDS             7409..8146
                     /locus_tag="ACHBKA_002278"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9445940.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="ACHBKA_002279"
     CDS             8136..8858
                     /locus_tag="ACHBKA_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9445941.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..>9361)
                     /locus_tag="ACHBKA_002280"
     CDS             complement(8942..>9361)
                     /locus_tag="ACHBKA_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9445942.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 a
//
LOCUS       DAYYOA010000107         9281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000107 DAYYOA010000000
VERSION     DAYYOA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.108"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHBKA_002281"
     CDS             <1..435
                     /locus_tag="ACHBKA_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9445943.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHBKA_002282"
     tRNA            complement(759..842)
                     /locus_tag="ACHBKA_002282"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHBKA_002283"
     CDS             1370..1870
                     /locus_tag="ACHBKA_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445944.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHBKA_002284"
     CDS             complement(2060..2404)
                     /locus_tag="ACHBKA_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM9445945.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACHBKA_002285"
     CDS             complement(2558..3478)
                     /locus_tag="ACHBKA_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9445946.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACHBKA_002286"
     CDS             3665..4156
                     /locus_tag="ACHBKA_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM9445947.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACHBKA_002287"
     CDS             complement(4180..6555)
                     /locus_tag="ACHBKA_002287"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM9445948.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACHBKA_002288"
     CDS             6881..7507
                     /locus_tag="ACHBKA_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9445949.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACHBKA_002289"
     CDS             7530..8234
                     /locus_tag="ACHBKA_002289"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9445950.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACHBKA_002290"
     CDS             8426..9259
                     /locus_tag="ACHBKA_002290"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM9445951.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DAYYOA010000108         9048 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000108 DAYYOA010000000
VERSION     DAYYOA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.109"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHBKA_002291"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHBKA_002291"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9445952.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="ACHBKA_002292"
     CDS             2714..2938
                     /locus_tag="ACHBKA_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445953.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="ACHBKA_002293"
     CDS             2951..3451
                     /locus_tag="ACHBKA_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9445954.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..5395
                     /locus_tag="ACHBKA_002294"
     CDS             3548..5395
                     /locus_tag="ACHBKA_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM9445955.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            5398..6294
                     /locus_tag="ACHBKA_002295"
     CDS             5398..6294
                     /locus_tag="ACHBKA_002295"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9445956.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            6344..6733
                     /locus_tag="ACHBKA_002296"
     CDS             6344..6733
                     /locus_tag="ACHBKA_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9445957.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            6720..>9048
                     /locus_tag="ACHBKA_002297"
     CDS             6720..>9048
                     /locus_tag="ACHBKA_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9445958.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         3196 a         1362 c         1909 g         2581 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     4681 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     4741 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     4801 ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact agtatactag aattactaga
     4861 tggatataac tctgatttga tttcagagta taaaaatgag attgaaacat gtgtaaacga
     4921 aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa
     4981 ggtagagctt gaaattgaag atttaaaaaa acaagtagaa aagctatctc ataagagtta
     5041 taaagatgtt tatgatgagt attcaaaaga gacagatatt tatagggaac tgaaacgtga
     5101 tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat agtgttgaca aaattgactt
     5161 tggcaatggt ataaaagtta cagacgaaat tgatgaacac cgcggagaat tgattaagaa
     5221 tttagtgacc aatcgggcta agattcaaac aataattaat gaaatgtcag atgaaattga
     5281 tagttataga gcagagctga ataaatcaga ttggaaagtt aactataatg aggtttcaaa
     5341 gaggtatcaa agaggtatca aaaacttagt gaagagttat ctcaagatga attaaaaatg
     5401 ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa ctgaagagcg attggaagaa
     5461 attattcaac aagaaataaa gcttaaagat taccaagttg aggttgactc agcaatgtca
     5521 agaatgaata aatcatatca aaatctctat tctgagagaa gtagttcctc gcgcgaaatt
     5581 ttttctgata ttacaggaat agaggctact attcaaccaa atagggattt caataattat
     5641 gttaaagagc tcagagcttg tattaataag gaggacactt ttgacagtga gtttggatct
     5701 cttattgata agctgacaaa aaattccatt actaatcaag atatatataa tgacatactt
     5761 gatataagag ttagtgggga aagtagtatt ttttcagata aaaaattaat caattctata
     5821 agaaaattga atgatgcaca agttcttgaa ttacaaaccc ttaagccaaa tgatttagta
     5881 gttatccgac ttgaactaaa tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa
     5941 aagacatctg caattctttc tatgattttg gcgtatggaa attcaccact catattggat
     6001 cagcctgaag atgatttgga tagtcaatta attaataact taatcgtaag aagtatcatt
     6061 acaaaaaagg aaaacagaca aattttaatt gtaactcata atgcaaatat cccagttaat
     6121 ggtgattcag aatggttgat tagcatggga gattcaaaag aaatatcggc tgatcagtgc
     6181 gggtcggtag atgaacccaa tatcaagcca agaatatgta ctgtgatgga aggtggcgag
     6241 gatgctttta caaatagagc gaaacgctat ggttttaaac aaattgtaga ataacactag
     6301 agggtgagac atattattat gtccgccctt tttaggagga aaaatgaaaa aaataaaaag
     6361 ctataccagt atctgggcag ttgaaaaagt catttacgca atcaatgatt ttcagttgcc
     6421 ttttcctgtg acttttaacc aaatggcttg gttcgtttta tctttactgt ttgtcatcgt
     6481 atttgcccat gtgcctccac tatctatgat agaggaggtc tttttaaaat acttaggcat
     6541 accggtggct gtcacttggt ttatgtcgca aaagacgttt gatggcaaga aaccactggg
     6601 atttttacga tcctttatca gctatcactt gcgatttaag gtgacttttg caggaaagaa
     6661 agtcaaagaa cagaagaaac gatgggatga acccagtact cttgtaagga gtgtgaacta
     6721 tgtatccgat taagtatatt gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg
     6781 cctactatga attagtgcct tacaattact cctttttgtc acccgaacag aaatttcaag
     6841 tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga aggcaagatc catgcgttac
     6901 aaattgccac agaaagtagt attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg
     6961 gtagactcaa agaagtagcg aaacaacgaa ttgatataca gacagatgca ttagtatcca
     7021 tgattggcga tagtcagatt gattatcgct tttttattgg ctttaaacta attgcgacag
     7081 atgaagaagt caatctaaag agtctcaaga aatctttctt ttctgggctt caagaatttg
     7141 tctatggagt gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc
     7201 gctgctattc taagcttgaa aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca
     7261 ggcgagtcac accgagtgac ctgacgtatt tgattgaaca tatttatggc gaaaaaggga
     7321 taccgtttga agaatacgaa tttcagctac caaagaaaaa gctaaaatca gaaacactgg
     7381 tgaaacgtta tgacttatta cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct
     7441 tacggatgga acatgagaat catgagtcgt acgtagcgta tttaacaatt aatacaattg
     7501 tgggggagat ggaatttcct tcctcagaac ttttctatta ccagcaacaa caatttacat
     7561 tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg
     7621 ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca tgcttaccaa tctgacaatg
     7681 aaacgaattc caatgtctta gatgctttgg attccgtgga tgaattggaa accaccttgg
     7741 atcagtcaaa agaatctatg tacaaactga gttatgtggt tcgagtgagt gccgaatcag
     7801 tggatgaact aaagcgacgc tgtgatgaag tgctcgattt ttatgatgat accaatgtaa
     7861 aactagtccg gccctttggc gatatgatgg ggctacatga agaattctta ccctcaagta
     7921 aacgatatat gaacgactat attcagtatg ttacgtctga ttttttggcg gggcttggtt
     7981 ttggggctac tcagatgcta ggagaattag aagggattta ctttggctac aatgtggata
     8041 caggtcgtaa tgtttacctg aaaccggcat tggcttcaca aggagtcaag ggttcagtaa
     8101 ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca
     8161 atctcttagt ctactatgct gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag
     8221 gggagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     8281 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     8341 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     8401 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     8461 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     8521 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     8581 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     8641 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     8701 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     8761 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     8821 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     8881 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     8941 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     9001 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaacg
//
LOCUS       DAYYOA010000109         9001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000109 DAYYOA010000000
VERSION     DAYYOA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.110"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            213..1427
                     /locus_tag="ACHBKA_002298"
                     /pseudo
     CDS             213..1427
                     /locus_tag="ACHBKA_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1606..1941)
                     /locus_tag="ACHBKA_002299"
     CDS             complement(1606..1941)
                     /locus_tag="ACHBKA_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9445959.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1925..2188)
                     /locus_tag="ACHBKA_002300"
     CDS             complement(1925..2188)
                     /locus_tag="ACHBKA_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9445960.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2648..3451)
                     /locus_tag="ACHBKA_002301"
     CDS             complement(2648..3451)
                     /locus_tag="ACHBKA_002301"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9445961.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3429..4553)
                     /locus_tag="ACHBKA_002302"
     CDS             complement(3429..4553)
                     /locus_tag="ACHBKA_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9445962.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4569..5384)
                     /locus_tag="ACHBKA_002303"
     CDS             complement(4569..5384)
                     /locus_tag="ACHBKA_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445963.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5374..6246)
                     /locus_tag="ACHBKA_002304"
     CDS             complement(5374..6246)
                     /locus_tag="ACHBKA_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9445964.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6331..7620)
                     /locus_tag="ACHBKA_002305"
     CDS             complement(6331..7620)
                     /locus_tag="ACHBKA_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9445965.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7764..8276)
                     /locus_tag="ACHBKA_002306"
     CDS             complement(7764..8276)
                     /locus_tag="ACHBKA_002306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445966.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8343..8486)
                     /locus_tag="ACHBKA_002307"
     CDS             complement(8343..8486)
                     /locus_tag="ACHBKA_002307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9445967.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2793 a         1906 c         1439 g         2863 t
ORIGIN      
        1 atgattatcc attgagagac tcctttggta ggatgacgtt ttggtcgact aaattttacc
       61 aaaagtgagt ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg
      121 attttccacc aacacaaaat attatagaag caaaatcaat tctaccttag atgttcttat
      181 atcactctat cagtcctatt tgacaaagag ccaagaaaaa gacgttggtc gtcgatgctg
      241 ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg
      301 ggaaagcaat tatagtgaaa aatgggaaga aggaaacgat tgtccgtttt gaacaataca
      361 atcatatgcc tttggttacg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgta
      421 cccattggac ggctcaaagt tattttgtcc gactcagcca ttcaatcgca gatcatgttc
      481 gatataaaat tgcttcttta ctgactgaaa aagtatcttt gtcttttatt gcgaaaagtt
      541 gtcaggtatc tttgactacc gttactcgta cattgaaaga gtttaaaagc tatttaccaa
      601 agccttctaa gaagattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt
      661 ccatagaaga taaaatgagc tttatttgcg cagatggtga aacaggaaac ttaattgctg
      721 tcttgcctac gcgtaaatta cctcgattaa caagctattt cttaggctgc accaatccag
      781 aagaagtaga attcttagtc acagacatga acgccgctta cttccagctt accaaacgtg
      841 ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tgtcaaacac atgaatcagg
      901 cactcaatga gttgcgtatc cgtgaaatga atgagcttcg taaagcagga cagaaaagtc
      961 aggcagaaaa actgaaaaag aactggcgct ttctgctaaa aaatcgtgca agcatcaacc
     1021 attatgagta caaaacatgg aaaagtttcc gagcaccaaa atacccattt ctcactgaag
     1081 caatgatgat tgatcgattg cttgagtttt ctgcgcctct aaaggaggcg tatccctttt
     1141 ttcatgaatt agtcgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg
     1201 cagaacttcc cgaaacgtta gatgacggct ttcgggagaa gcttcaaaac cttctgacct
     1261 atgaagaagg tatcaccagc gcaatgatct atccttattc caatggaaaa attgaagcga
     1321 agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca ttcgagaaca
     1381 tgagaattag aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgaa
     1441 ccagaatgtg gttcattctg acccggattt gattttgcac aactcatcag tccttattga
     1501 caaagagcca aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac
     1561 aacccatcag tccttattga caaagagccc ttctttttag tattattata attcttccat
     1621 gcgttcttcc agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac
     1681 ctttttgttg tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt
     1741 tagtcctgcc tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt
     1801 agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat
     1861 atagacaggc cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat
     1921 atcattagta ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt
     1981 ggacaggcaa tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca
     2041 attcaataga aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg
     2101 ctgttttggg gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt
     2161 cgtccataga aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata
     2221 tcacggaaag agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg
     2281 tatttatcta aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt
     2341 tcaaaatccg ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag
     2401 actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg
     2461 ctctggtaaa aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct
     2521 ccaccctttt tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca
     2581 agtggaacca gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca
     2641 ataattttta atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg
     2701 gagttaaata atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc
     2761 ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt
     2821 cagccataaa ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta
     2881 caaaaatacc taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag
     2941 ttacaataga tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt
     3001 cagcaggtgt acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt
     3061 cgtgctgggt atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac
     3121 aatggatata ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc
     3181 gacgatcatg attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca
     3241 ttttccgtaa gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata
     3301 gaataaaatc gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg
     3361 gatttttaat tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa
     3421 aatggatatc agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa
     3481 tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct
     3541 atgcggagat tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt
     3601 tctataccat cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc
     3661 acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct
     3721 aaatacaatg agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa
     3781 aattgactgg ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct
     3841 ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc
     3901 gtatcaatca tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc
     3961 gcttcaattt gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt
     4021 accaattctt ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct
     4081 aataaaaaat aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc
     4141 cctcctgaca aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa
     4201 gcttcttcca aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa
     4261 ataatattct cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg
     4321 atctctcgtt ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca
     4381 ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat
     4441 ggtcccagta acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg
     4501 gtatgttctc catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt
     4561 cctccaattt atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt
     4621 gcatacaaga ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa
     4681 tttgtcccac cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct
     4741 actgtccgta aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa
     4801 ataccggtag aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt
     4861 gtttgtttga tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt
     4921 gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa
     4981 gtagtatcaa ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta
     5041 aaaggaatga acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg
     5101 caataagtaa aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt
     5161 gtagcaataa aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag
     5221 caaaaatttt ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct
     5281 ttcaatggct taaaagaatt ggccataata aacaataatg gatagataat aatgattatg
     5341 atgaacataa ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc
     5401 cacaaattta aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat
     5461 tgctgaagat tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac
     5521 gtttgcacta ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata
     5581 ctgcagacct tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg
     5641 caacacaatt gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat
     5701 catctcttga tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac
     5761 tttccaagca gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat
     5821 aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa
     5881 tatccatgta aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa
     5941 tggtttatac aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata
     6001 tgggaacaca cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac
     6061 tttatgaaag actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt
     6121 agtgggggcc accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata
     6181 aaaaacaaac acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg
     6241 cttcatttcc gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt
     6301 cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg
     6361 agtagctttc atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa
     6421 caatttttgt tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt
     6481 atccatctga tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc
     6541 attttgggcg tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt
     6601 agctaaagat tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt
     6661 atccgtttgt gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc
     6721 ttcccaactt ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc
     6781 cctttgagca atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc
     6841 ttgttcccac gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata
     6901 agcagcgata ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc
     6961 attactttct aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc
     7021 atactttcca gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt
     7081 tggaccattt tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg
     7141 attactaatg gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat
     7201 attgtctaaa aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa
     7261 agattgagga ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac
     7321 ttgtactacg tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc
     7381 tttatacata tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta
     7441 tttatctact aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata
     7501 ctctatcgtt acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc
     7561 tgccaaagag atgacactta aaacagacac aacccctata acaagatttt tctttaacat
     7621 aacattaact tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac
     7681 taccaatgta aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg
     7741 tacagggtca ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc
     7801 ccatctccaa atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct
     7861 ttcttccata aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca
     7921 aggtactcaa tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc
     7981 tgccccactt cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg
     8041 ctttgatagg ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt
     8101 tttttccctt cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc
     8161 caagataatt gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc
     8221 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     8281 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     8341 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     8401 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     8461 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     8521 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     8581 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     8641 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     8701 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     8761 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     8821 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     8881 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     8941 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     9001 g
//
LOCUS       DAYYOA010000110         8990 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000110 DAYYOA010000000
VERSION     DAYYOA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.111"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHBKA_002308"
     CDS             278..787
                     /locus_tag="ACHBKA_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9445968.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHBKA_002309"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHBKA_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9445969.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACHBKA_002310"
     CDS             2322..3302
                     /locus_tag="ACHBKA_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9445970.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACHBKA_002311"
     CDS             3424..4842
                     /locus_tag="ACHBKA_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9445971.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACHBKA_002312"
     CDS             4913..6382
                     /locus_tag="ACHBKA_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9445972.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACHBKA_002313"
     CDS             6392..6859
                     /locus_tag="ACHBKA_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9445973.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACHBKA_002314"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACHBKA_002314"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9445974.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>8990
                     /locus_tag="ACHBKA_002315"
     CDS             7848..>8990
                     /locus_tag="ACHBKA_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9445975.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECA"
BASE COUNT         3099 a         1335 c         1806 g         2750 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca
//
LOCUS       DAYYOA010000111         8881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000111 DAYYOA010000000
VERSION     DAYYOA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.112"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..471
                     /locus_tag="ACHBKA_002316"
     CDS             <1..471
                     /locus_tag="ACHBKA_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM9445976.1"
                     /translation="AKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETKSLFL
                     KDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQ
                     IPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            508..1518
                     /locus_tag="ACHBKA_002317"
     CDS             508..1518
                     /locus_tag="ACHBKA_002317"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM9445977.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1633..2346)
                     /locus_tag="ACHBKA_002318"
     CDS             complement(1633..2346)
                     /locus_tag="ACHBKA_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445978.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2519..3126)
                     /locus_tag="ACHBKA_002319"
                     /pseudo
     CDS             complement(2519..3126)
                     /locus_tag="ACHBKA_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            3610..3837
                     /locus_tag="ACHBKA_002320"
     CDS             3610..3837
                     /locus_tag="ACHBKA_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM9445979.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3853..5019
                     /gene="hemW"
                     /locus_tag="ACHBKA_002321"
     CDS             3853..5019
                     /gene="hemW"
                     /locus_tag="ACHBKA_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM9445980.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5253..6296
                     /gene="hrcA"
                     /locus_tag="ACHBKA_002322"
     CDS             5253..6296
                     /gene="hrcA"
                     /locus_tag="ACHBKA_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM9445981.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6390..6953
                     /gene="grpE"
                     /locus_tag="ACHBKA_002323"
     CDS             6390..6953
                     /gene="grpE"
                     /locus_tag="ACHBKA_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM9445982.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7004..8833
                     /gene="dnaK"
                     /locus_tag="ACHBKA_002324"
     CDS             7004..8833
                     /gene="dnaK"
                     /locus_tag="ACHBKA_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM9445983.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
BASE COUNT         3045 a         1513 c         1847 g         2476 t
ORIGIN      
        1 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
       61 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
      121 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
      181 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
      241 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
      301 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
      361 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
      421 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
      481 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
      541 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
      601 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
      661 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
      721 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
      781 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
      841 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
      901 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
      961 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
     1021 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
     1081 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
     1141 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
     1201 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
     1261 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
     1321 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
     1381 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
     1441 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
     1501 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
     1561 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
     1621 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
     1681 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
     1741 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
     1801 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
     1861 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
     1921 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
     1981 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
     2041 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
     2101 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
     2161 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
     2221 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
     2281 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
     2341 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
     2401 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
     2461 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
     2521 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
     2581 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
     2641 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
     2701 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
     2761 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
     2821 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
     2881 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
     2941 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
     3001 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
     3061 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
     3121 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
     3181 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
     3241 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
     3301 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
     3361 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
     3421 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
     3481 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
     3541 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
     3601 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
     3661 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
     3721 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
     3781 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
     3841 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
     3901 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
     3961 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
     4021 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
     4081 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
     4141 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
     4201 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
     4261 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
     4321 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
     4381 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
     4441 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
     4501 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
     4561 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
     4621 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
     4681 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
     4741 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
     4801 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
     4861 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
     4921 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
     4981 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
     5041 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
     5101 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
     5161 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
     5221 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
     5281 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
     5341 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
     5401 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
     5461 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
     5521 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
     5581 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
     5641 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
     5701 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
     5761 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
     5821 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
     5881 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
     5941 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
     6001 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
     6061 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
     6121 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
     6181 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
     6241 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
     6301 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
     6361 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
     6421 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
     6481 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
     6541 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
     6601 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
     6661 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
     6721 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
     6781 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
     6841 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
     6901 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
     6961 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
     7021 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
     7081 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
     7141 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
     7201 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
     7261 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
     7321 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
     7381 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
     7441 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
     7501 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
     7561 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
     7621 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
     7681 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
     7741 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
     7801 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
     7861 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
     7921 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
     7981 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
     8041 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
     8101 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
     8161 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
     8221 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
     8281 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
     8341 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
     8401 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
     8461 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
     8521 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
     8581 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
     8641 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
     8701 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
     8761 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
     8821 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
     8881 t
//
LOCUS       DAYYOA010000112         8494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000112 DAYYOA010000000
VERSION     DAYYOA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.113"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHBKA_002325"
     CDS             complement(<1..149)
                     /locus_tag="ACHBKA_002325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9445984.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHBKA_002326"
     CDS             complement(593..1714)
                     /locus_tag="ACHBKA_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM9445985.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHBKA_002327"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHBKA_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM9445986.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHBKA_002328"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHBKA_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM9445987.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHBKA_002329"
     CDS             complement(4304..5215)
                     /locus_tag="ACHBKA_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9445988.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHBKA_002330"
     CDS             complement(5232..6239)
                     /locus_tag="ACHBKA_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9445989.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHBKA_002331"
     CDS             complement(6236..8365)
                     /locus_tag="ACHBKA_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9445990.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHBKA_002332"
     CDS             complement(8370..8480)
                     /locus_tag="ACHBKA_002332"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9445991.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAYYOA010000113         8242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000113 DAYYOA010000000
VERSION     DAYYOA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.114"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..600
                     /gene="lepB"
                     /locus_tag="ACHBKA_002333"
     CDS             49..600
                     /gene="lepB"
                     /locus_tag="ACHBKA_002333"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9445992.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(683..1225)
                     /locus_tag="ACHBKA_002334"
     CDS             complement(683..1225)
                     /locus_tag="ACHBKA_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9445993.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1462..1860)
                     /locus_tag="ACHBKA_002335"
     CDS             complement(1462..1860)
                     /locus_tag="ACHBKA_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9445994.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2120..2980
                     /gene="ylqF"
                     /locus_tag="ACHBKA_002336"
     CDS             2120..2980
                     /gene="ylqF"
                     /locus_tag="ACHBKA_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM9445995.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            2973..3740
                     /locus_tag="ACHBKA_002337"
     CDS             2973..3740
                     /locus_tag="ACHBKA_002337"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM9445996.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3796..4653
                     /gene="dprA"
                     /locus_tag="ACHBKA_002338"
     CDS             3796..4653
                     /gene="dprA"
                     /locus_tag="ACHBKA_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM9445997.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4775..6853
                     /gene="topA"
                     /locus_tag="ACHBKA_002339"
     CDS             4775..6853
                     /gene="topA"
                     /locus_tag="ACHBKA_002339"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM9445998.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6822..8198
                     /gene="trmFO"
                     /locus_tag="ACHBKA_002340"
     CDS             6822..8198
                     /gene="trmFO"
                     /locus_tag="ACHBKA_002340"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM9445999.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2881 a         1446 c         1733 g         2182 t
ORIGIN      
        1 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
       61 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
      121 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
      181 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
      241 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
      301 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
      361 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
      421 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
      481 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
      541 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
      601 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
      661 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
      721 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
      781 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
      841 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
      901 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
      961 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
     1021 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
     1081 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
     1141 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
     1201 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
     1261 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
     1321 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
     1381 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
     1441 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
     1501 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
     1561 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcgttttc
     1621 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
     1681 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
     1741 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
     1801 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
     1861 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
     1921 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
     1981 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
     2041 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
     2101 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
     2161 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
     2221 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
     2281 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
     2341 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
     2401 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
     2461 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
     2521 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
     2581 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
     2641 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
     2701 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
     2761 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
     2821 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
     2881 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
     2941 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
     3001 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
     3061 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
     3121 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
     3181 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
     3241 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
     3301 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
     3361 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
     3421 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
     3481 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
     3541 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
     3601 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
     3661 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
     3721 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
     3781 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
     3841 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
     3901 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacatcg ggagctgttt
     3961 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
     4021 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
     4081 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
     4141 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
     4201 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
     4261 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
     4321 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
     4381 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
     4441 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
     4501 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
     4561 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
     4621 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
     4681 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
     4741 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
     4801 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
     4861 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
     4921 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
     4981 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
     5041 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
     5101 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
     5161 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
     5221 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
     5281 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
     5341 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
     5401 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
     5461 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
     5521 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
     5581 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
     5641 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
     5701 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
     5761 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
     5821 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
     5881 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
     5941 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
     6001 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
     6061 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
     6121 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
     6181 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
     6241 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
     6301 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
     6361 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
     6421 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
     6481 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
     6541 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
     6601 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
     6661 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
     6721 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
     6781 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
     6841 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
     6901 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
     6961 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
     7021 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
     7081 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
     7141 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
     7201 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
     7261 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
     7321 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
     7381 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
     7441 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
     7501 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
     7561 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
     7621 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
     7681 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
     7741 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
     7801 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
     7861 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
     7921 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
     7981 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
     8041 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
     8101 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
     8161 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
     8221 gacggatagt ctcgctcttt tt
//
LOCUS       DAYYOA010000114         8235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000114 DAYYOA010000000
VERSION     DAYYOA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.115"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..249)
                     /locus_tag="ACHBKA_002341"
     CDS             complement(<1..249)
                     /locus_tag="ACHBKA_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9446000.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRK"
     gene            complement(246..767)
                     /locus_tag="ACHBKA_002342"
     CDS             complement(246..767)
                     /locus_tag="ACHBKA_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9446001.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(769..1803)
                     /gene="sppA"
                     /locus_tag="ACHBKA_002343"
     CDS             complement(769..1803)
                     /gene="sppA"
                     /locus_tag="ACHBKA_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9446002.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1836..3251)
                     /locus_tag="ACHBKA_002344"
     CDS             complement(1836..3251)
                     /locus_tag="ACHBKA_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM9446003.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3220..5373)
                     /locus_tag="ACHBKA_002345"
     CDS             complement(3220..5373)
                     /locus_tag="ACHBKA_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM9446004.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5631..5867
                     /locus_tag="ACHBKA_002346"
     CDS             5631..5867
                     /locus_tag="ACHBKA_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM9446005.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5886..6101
                     /locus_tag="ACHBKA_002347"
     CDS             5886..6101
                     /locus_tag="ACHBKA_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM9446006.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(6804..7556)
                     /locus_tag="ACHBKA_002348"
     CDS             complement(6804..7556)
                     /locus_tag="ACHBKA_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9446007.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(7571..>8235)
                     /locus_tag="ACHBKA_002349"
     CDS             complement(7571..>8235)
                     /locus_tag="ACHBKA_002349"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9446008.1"
                     /translation="EQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIA
                     NQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICC
                     KFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISN
                     SCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVIL
                     LF"
BASE COUNT         2612 a         1473 c         1185 g         2965 t
ORIGIN      
        1 ttttcttact aaaaaaccta ttatgaaaat cattatcgaa aaaactaggg taattcctcc
       61 aaataaaaca acattcccgc ttagattgac taacatatct atatccttac tggttagttc
      121 tgtagtattt aaagtagttg aatcaataga agatagaaac ataccaccca tcaatgctaa
      181 aagactacat aaaatccctg ttattccact aattaaaatc aaaataaact ctgtttttct
      241 cttattcatt taattctctc cagattaagt gttttagaat tagaaatttt ttgaccatag
      301 ttaatagctt ctaatgaacg aataacaacc actgacgtgt tagaaaaata atccccaata
      361 tgttgtttct tgggcgtgaa agcagccacc aaatacccaa tttgaaatac aaaatttttt
      421 aatataaacc tacatataca ctcacgaata aagatagttt gccaacttag ctctttttcc
      481 tcaaagctca ctactcttat cccaaaaatc attttcccaa ttgtttgtcc tgaattcagc
      541 ttagtcaaaa gtacaaaata ggttaaataa atagctaatg acgctatacc ataaaaagta
      601 ataatggatt tgtcagattc cagtcccatg attttgtaaa agagacctat agtagcatgg
      661 gtgatgatgc taatacaaat taaatcaata agaaaagcaa aaaatcgtaa ccaaaatccc
      721 gcaaatacat aattaggcaa agtttcaatg ccactcttct tattcatttt atagccctcc
      781 atagtaatac attggttttg gagtatcaga aaatccaagt ttttcaaaaa ctgaaattac
      841 acgatctgat gtagttgcct ttagcccttg ccattcagca atcttattac ctaaccaaga
      901 agaagcaaag ccagttgtgt catttttata ttctataacc gtcgcatcag ttaatttctt
      961 ctcttttttt agggaatcta atgcatcttc agagtaacca atttgatcca ccaacccatt
     1021 ttctactgct tggttaccat cataaattct tccatccgca agttctttca ctgcattagt
     1081 atccatattt cttccttttg ctacaacatt gacaaaacga tcataagcac tcatcacaaa
     1141 ttcctgtata actttattct cttcctctga aggtttgtgt tgaggtgaca tcatatcttt
     1201 taatgcacca cttttatacg tcgcatcagt tacgccaagt ttttctaata agccagaata
     1261 atttaaacca gaaataatga caccaattga tccagttgtt gtttcttccg tagcaaatat
     1321 tttatctgaa ccagcagaaa tgtagtaccc tccacttgcc gctgtatttt taaatgcagt
     1381 atagatagga atatctagct ttttaatttt tgccatttct ttagcaatct cagcactttc
     1441 atatattcct cccccaggtg aatttacttc taataacact cccttaaccg ctttgtcttc
     1501 ttgaattttc tttagctgtg ttaaaaaatt ctgatggtta tattgttccc tactaaaaag
     1561 attgctttca cctgtatctg caataactcc attgacggat agtttgacaa tctttttatt
     1621 tgatgcgcct tcttctaacg tttcttctgt gatttcgtta gatccgtaaa aaatcttatt
     1681 tattcctgtt agggttgtat ttgatgcttc ctctttatct tttcctggaa ttagcgcgac
     1741 gataatggat agtaacaata atccgcacgc tactcccaca gcaatccatc ttcttttatt
     1801 catataaact cctcctatat tcttagtaaa aataattatt ttttatttag aaacacatcg
     1861 acaatttgtt gccaatatgt tttttctcct acaattaaag acaactctcc agttaatcca
     1921 taacgaaacg gaaacgattg agttgttttt aacgttcctt tcactgcata aaaaacacct
     1981 ttctcagtgg cagtagaatt ttctgaaatt tcatctaaag ttccgtcgat cgtaatcgga
     2041 gccccacctt tttgatccaa tttaaaatga accttcatcc cttctttaat ataggtactt
     2101 tcattcgcag gtaaaagtgc tgtaaatttc atttttgatt tttcgtggat cggataaatt
     2161 tctgcaagca ctgttccttt ggctagttct gcttgtccct ttgtttcttc atttaaatga
     2221 accgttccag atacaggagc ttttagtatt ccttttgaaa gttgatcgtc caaacttttg
     2281 ataactgact ggttcttctc ttgtttttca tttagctctg taactgtttg ttttacagtt
     2341 gttattgctt gttctagctc ttgctttcct ttttccgttt gactaattct gtcattctca
     2401 gaagtagttg gtttaactaa tttagctttt tctgactgat actgttctac ttcttttttt
     2461 agttgctcaa tttgttcatt tatgcttgct gaaattgtca gtttcacttg atttttttga
     2521 tcatcagaaa cattatttag ttgttcttgc caattctcat agtttgccat catttctttt
     2581 gaaaaatctt ttaatgattg attattactc caagctacct gtacttgttg ccattcttgt
     2641 aatttactat ctgaactctc aatttgtttt tctagttgtc tttttgtctt ttcgtatact
     2701 tctaattgct tttgatcatt taattcactt tgcttcaaag cgtagcggag tgattctttt
     2761 tctgacaaca tgctctttaa ttgattactg tagccaaaac tgtcatccgt tgaaaacaaa
     2821 ttttttcctt tacttatact ttcaataaat agctgagcca tctttatttg ttgagtaata
     2881 cgttggtttt cttgttctat ctgagccttt tcattctcta ttaaagaaca atccaatcta
     2941 accaacacat cgtccttatg aacaaattga ttttctttta ggaagttttc cattatttta
     3001 gcttctaatg gcacttgtaa cttctctatt ttttcggggt tcaattgagc cgttgtccga
     3061 ataacaacct cttttttagc aaaggttaaa aacaatccaa taatacataa aaaaagaatt
     3121 acagggtata acacccaacg gtagaagcta tgatgttgtt tagtatagat actagaatgt
     3181 tcgaaccaat tattattctg cataaattgt ttccctctcc tagatttccc ataatttttg
     3241 atagatacca tcggcaaaac gaagttcatc gtgcgttcct ttttctacca aatgcccttc
     3301 atggagaact aaaatttgat cacatgcttt tgctattggt aaatgatgcg caataaaaat
     3361 aatcgttttt tcttttaaat tgaggagata attcataatt gcatgttcta acaaggtatc
     3421 taatccacta gttgcttcat ctaaaattaa tatgtccgga ttttttaata aagcccgtgc
     3481 taaggctaat cgttgtcgtt gccctcctga aagattattt ccaccttctt ccaacaatgt
     3541 ctcgtaacgt aatggctgtt gattaataaa ttcatttagt tggactatct cacaaacttc
     3601 tattatttgt tcgaaacttg gtgggctatc taaaccgaat ataagattct caatgattgt
     3661 tccactaaag aaaaatgact cttgaggcaa atatgtcact cgatcgcgta atatctgaaa
     3721 aggaatgtct aagtgattaa tgtttccata atggatcgat ccttctgatg cttcataaaa
     3781 acgaacaaat aactttgcaa gagttgattt tccagaacca ctaactccaa ctatagccgt
     3841 tttactgtgc gaaggaattg tacatgaaat ttcgttcaac gttggggcct tcatgctata
     3901 cgagaatgaa acgtttttta cagtgatatc ttgttgaaat aattgttcag aaagcatata
     3961 ttcaaaagac tggttcgatt tttctgaatc aatcgcgaaa atttcattta aacgattatt
     4021 tgcaacttga gccgtttgca ttttcacttg taaattaata atgttttgta aaggatctgt
     4081 caaaaatact agtaaagcat tgtatgtgat aagttgacct agactaattc tattatccat
     4141 aacaaaataa gaacctagcc ataaaattaa tgcactgcta attaattgaa tcaaatgttt
     4201 aattccttgt tgaatattat caagcgtaac cgtacgaaat gattttttca ttaatttaac
     4261 aaattcatgg tctatacggt tatatacttt ctcttcaccg ctatatgctt taattgtttc
     4321 tattccttta agactttcta tgatactaga atttacagct gcaccggctg ccatttcttc
     4381 cctgttcgat ttttcatagc ttttcacgaa agcgaatatt gcaactacat aaaaaggcaa
     4441 agagcataga gtgattaaaa acaacgtact attctgaata gctaacgtaa ccccaacaag
     4501 caacaccata ccaatatcta aaaatattga taacgtagca cttgctaaag catcaataat
     4561 cttactggca tcaagaaaac gagaaataat ttcccctgat tttctcgtag caaaaaaatt
     4621 cattggtaaa gttaacacat gtttgaaata ctgaagcata attgccatac tcattcgctg
     4681 tcctaaaata ataagaagat aactacgact atattcaaac aatacccgaa ataaatagac
     4741 agcgatcaag ccaatggaaa ttatatttaa agttgaacgt gcttgattag ggatgaaaaa
     4801 atcaagaata ccttgaaaat aatatgagct tcctatgcca aaaaacgtaa tgaaaatcga
     4861 tgctaaaacg atgtggaata ctaatccttt ttgtttccaa attataggca aaaaagaact
     4921 taatccagct actttttctt taataggaat ataagtttct ctaggcatag gtaataaaag
     4981 aatacctgtc cattcttttt gaaacgcttc aatcgttttt ctattttttc cattagcggg
     5041 atctgcaatc catagtacgt ttcccttcgt tttataaatt acaacgtagt gcatatagct
     5101 gttatcaacc aatatatgtg caattaatgg taagataaga tctctttctt tccagatctc
     5161 actatctgct tgaatagcca tacaatcaaa agacaatttt tcaaatgtct ttttcaatcc
     5221 aaatgctgaa gtaccctcta aatcagtccc agataggtcc cgtaatttat gaatcgggat
     5281 atcagtttta aaatatttta aaatcatcga aaggcaagca acaccacaat ctttttcatc
     5341 ttgttgctgc acaaaaggaa atgattttat cacttatcta acacaactcc ttctaaaaaa
     5401 tttttataga aaaattctca atatagcttt aatacagtga aaatattgtc tcataattcc
     5461 acaaaaactt tgaaaacagt tcttgaacac taactttttc attcctgaat caaatttatt
     5521 ttggaaattt tactattcaa gaataaactt gataagtttc aggaagattt ttactgttaa
     5581 tcaaatacgt atgttacagt agcaccgtaa aaaatattat ttaaagaagc gtgtttaaac
     5641 atgcaaaatg taaaggaact aagtattgtg gaaatgcaca aactgttggt ggaggcaata
     5701 atgcttgggg aaaattgggg caagttgtag gtggacttac cacaggggca gtaggtggtg
     5761 caggtttagg aacagcaatt tgcggaccag cttgcggtgt tgttggaggg ttatatggag
     5821 ctgtcgctgg aggtgctgca gctggctggg atgctcgaaa aaaataaaaa aggagaataa
     5881 gtagtatgca tattaaaaac acaaaaacaa catttatatt atcttcagaa gagttgaaaa
     5941 atattcaagg tgggagtgcc gtgggagttt taggcactac tttttctggg gcaacagcag
     6001 gagtaaaact ttgttcagcc ggaggacctt atgcaatagc tgcttgtggt gtaggtggag
     6061 ctttattagg tgctggattc agcatgtgga ccggtgcgta aacaattaaa aaatatcaaa
     6121 cctcaagatt ggtttgtgtc gattacgaac ataggtatgt tctttatctt ttcaaaagtt
     6181 tggaatggac agaacttttt actaaagctt cttgtgctag tttttataat tatattaaat
     6241 ttttatcttt tattaaagtc agataaaaat tctttggaaa tgtaatggta gagatatttt
     6301 tacaaaagga cttgcagcgc tgtagttggt gctgcaggag gacctatttc ggccgttaca
     6361 ggaggtattg gtaatgtact tgtcggatgt gcattaaagc ctgttcctgc aagttaaatt
     6421 tataaaaaca ggaaagtaat tatctcaaat cctatcaagt acaatcatag gattgcactt
     6481 gacaggacaa attttttaac taaaaaatga aaaacacttt ctaaaaatta gtagaatttg
     6541 ccaaatcttt cgcatcttcc accatacatt taattttaat tcccctttat ggtacttaat
     6601 aaaaacaaga aaagaactat tcatttggcc cttttttggt tccagaccat agaaatattc
     6661 ctccagtaac taatattgct gttatgcacg taactaataa cagctataaa aattggtaca
     6721 actttaatag gattcagaaa aatttgcact agtgaaggaa tctattttat tagaaaaatg
     6781 caatctccct aaagaaaaaa ccactaaaca gtgctttctt tcaacttctc cttaattttt
     6841 tgtgctttcc ctaaagtaaa gtttctggcc aactctgaat caaaatatag tgaacgtttt
     6901 ttaaaatcta tttctttaac gttaaagata tttactaaac acgctcgact aattcttgtt
     6961 aacataggat atttcttttc aagctcacta attcttccat aaaattcgta ctgcccatct
     7021 ttagtacaca atgataatct atgtggtaat tttgaagctt ctacaaaata aatgtcattt
     7081 atatcaaaag taaatgtttg agaacctatg gaaaaaacaa atgcttgatt cgtaacctgt
     7141 tttgttgcat ctatcctttc ttgcgctaat aacaataact caacaatctc gtttcgatac
     7201 tcatctagtg tttgatcttt tgttacaaat cctaacgttt caactcttct ttttattgtt
     7261 acgggtaaca tctcatcatg agtagttgta aaaatgattt ttgcttgaac atcatatttt
     7321 ctgattactt ctgctaattc tataccgtta acttcatgat ttaaatcaat atctaagaaa
     7381 tatattcctt gctttggtcg gaattgtttg aggtattttt taacttctaa cggactttgt
     7441 gttttcaata ctatcttaaa tactttatcg tgaaaaagaa tgaaattatc aataattcgt
     7501 tctaactgat ttaattgtat aatttgatct tcacataata ttattggata actcataaat
     7561 cctccttccc tcaaaacaac aaaataactt gagtagtaaa ttgatacgga tcgttttgat
     7621 agttcacaaa catattggga ttctttttat tgatttcctc tatactagat aaacctaacc
     7681 cttgatgtcc cttttttgtt gttattcctt ttttcattag agtatctata gagagtagta
     7741 tctctctaca actattcgat atcgaaattt ctatctgttt atcatctcgg taaatagcta
     7801 gagacagttc tctatcttga ctctctatag atgcttcaat tgcattatcg agaataattc
     7861 caagcaccct aatacagtca aatattggaa caggaacttc aataatagta cttggacatt
     7921 cgaatttaca acaaatctta ttgttatttg cttggtatag cttcgctatc aacaaacttt
     7981 tcaaatattc attttttata ttttttaaat aacgatattc aaacttattg gttagataag
     8041 aattagaata gatctctaat tcttttattt ctttttccaa tatcgaatca tggttttgat
     8101 tagcgatttc ttttaaactc agcaataaat ttttatagtc atgtctaaat ctggcaaatt
     8161 tttcttgatc tttttccagt gaatcagtat atttttttag ataaaccaat tcttttcttt
     8221 tcaattgctg ttcat
//
LOCUS       DAYYOA010000115         8144 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000115 DAYYOA010000000
VERSION     DAYYOA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.116"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1248
                     /locus_tag="ACHBKA_002350"
     CDS             94..1248
                     /locus_tag="ACHBKA_002350"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM9446009.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="ACHBKA_002351"
     CDS             complement(1270..2022)
                     /locus_tag="ACHBKA_002351"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9446010.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="ACHBKA_002352"
     CDS             complement(2386..5052)
                     /locus_tag="ACHBKA_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9446011.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="ACHBKA_002353"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="ACHBKA_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9446012.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACHBKA_002354"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACHBKA_002354"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM9446013.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2368 a         1693 c         1452 g         2631 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagt
//
LOCUS       DAYYOA010000116         7974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000116 DAYYOA010000000
VERSION     DAYYOA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.117"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..876
                     /locus_tag="ACHBKA_002355"
     CDS             175..876
                     /locus_tag="ACHBKA_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9446014.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1485..2501)
                     /gene="tsaD"
                     /locus_tag="ACHBKA_002356"
     CDS             complement(1485..2501)
                     /gene="tsaD"
                     /locus_tag="ACHBKA_002356"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM9446015.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2498..2962)
                     /gene="rimI"
                     /locus_tag="ACHBKA_002357"
     CDS             complement(2498..2962)
                     /gene="rimI"
                     /locus_tag="ACHBKA_002357"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9446016.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2969..3511)
                     /gene="rimI"
                     /locus_tag="ACHBKA_002358"
     CDS             complement(2969..3511)
                     /gene="rimI"
                     /locus_tag="ACHBKA_002358"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9446017.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3495..4217)
                     /gene="tsaB"
                     /locus_tag="ACHBKA_002359"
     CDS             complement(3495..4217)
                     /gene="tsaB"
                     /locus_tag="ACHBKA_002359"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM9446018.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4408..5388)
                     /locus_tag="ACHBKA_002360"
     CDS             complement(4408..5388)
                     /locus_tag="ACHBKA_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9446019.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5412..6896)
                     /gene="galT"
                     /locus_tag="ACHBKA_002361"
     CDS             complement(5412..6896)
                     /gene="galT"
                     /locus_tag="ACHBKA_002361"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM9446020.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6908..7897)
                     /gene="galE"
                     /locus_tag="ACHBKA_002362"
     CDS             complement(6908..7897)
                     /gene="galE"
                     /locus_tag="ACHBKA_002362"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM9446021.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2244 a         1709 c         1403 g         2618 t
ORIGIN      
        1 caaatcattc gcttcaaaac aaattattca ataccattgt atatttttta cataataaca
       61 aattatattt gtaaagtatt aaaacttgtt tctaatgttg gtatgttaaa actttacaaa
      121 ttctttaaaa aaagtgacaa taaatatagt gaacaataag gagaggactt tttaatgcgt
      181 gttaataaaa tcgattttca cttttcaaaa ctcagatatc aacctgattt tgaacatttc
      241 tcggatgaag agttccaaca gatcaaggaa catactgttc ttcgttctta taaaaaagga
      301 cagatccttt tcgacgatgg tgatcccaga caacggttgt atatcttgac caaaggactc
      361 gttcgtttag aacgttatga tgaaagtgca acttattact actatgacta tgtgaataca
      421 aataaccttt ttcctatgtc aggaatgttt ggagaagaga cctacactta tacagctcaa
      481 gcgatgactg atattgaaac attctacctt cctgtttcct actatgaaaa attagcacga
      541 aaaaatcccg aacagttgat ctatatgata caaaaaattt ctaaagtagt tgaaatacat
      601 gaattgcgta tccaaatcgg attgacctct agcgcctttg accgggtaaa acaaaattta
      661 ttcattttga aagaagaatt gggcgtacca acaaaagatg gtcatatcac cattccttat
      721 ccgatcacct taaaagaatt agcagtgaat agcggcacga cacgtgaaac tgctggacaa
      781 gtaatcaaac aactacgtga ttccgggctg ttagattatg acaaaaagcg ttttactttc
      841 tgtaagacag ctactactcc agctatgaga gaataacgcg aacaaaaata agcagtgacc
      901 ctgataaata tcagggtcac tgcttatttt cctccctaaa acttctgaaa gatccggcag
      961 ttttagatca ctggacaaga ctcaaagatt ttctatgtgt gtttcagtta tgtaatattc
     1021 actgtttgca aaaaatactg ttagattaac tagatgaagc gtcttgtcct catccgatct
     1081 agattattcg atattcctcg acaataaacg attactcagt gagggcaatc agaggaacgg
     1141 ataatgataa ataagctgta aaaaatagca aaataacaat aatgattaaa agtatgtaaa
     1201 aaatagggct ttgacaaaaa cgtgtcacag ccctatttcg attaaacggt gtgtacctgt
     1261 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
     1321 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
     1381 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
     1441 gctgttctta ttacttgatg cagggcagct ttttcacaac ctctttattc agataaaacc
     1501 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
     1561 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
     1621 tctacctcag ggagtttagc tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
     1681 gccgctacac ctccagctat aatcagttgc ttgactggat aattttgaca agctcgaagt
     1741 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
     1801 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
     1861 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgcggaaa atggtaatta
     1921 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
     1981 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
     2041 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
     2101 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
     2161 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
     2221 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
     2281 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
     2341 tgatggcggc tagctacttc aggtactacg ccaccaaaac gtttatgact gtttatttga
     2401 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
     2461 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
     2521 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
     2581 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
     2641 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
     2701 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
     2761 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca gctaaaagca
     2821 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
     2881 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
     2941 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
     3001 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaacccca
     3061 atttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
     3121 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
     3181 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
     3241 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
     3301 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
     3361 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
     3421 aacgacgatc gatttgttcg actgtttttt ctgtataaat cattttatct tgaaaaaagc
     3481 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
     3541 aaccattttt cttctgcttc tactcgtttt aaatagcgag gaagaaaagc ttgtttctct
     3601 gcaactggtt caactaaagc acctaattga gccacagtta caccacttgg aatatcccat
     3661 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcaga gaagaatttg
     3721 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
     3781 tcagaagcag aaatatgtac atcttcaagt acagtttcta atctattttc tttccagcgg
     3841 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aatattatcc
     3901 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
     3961 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
     4021 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
     4081 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
     4141 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
     4201 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
     4261 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca ataatacatg
     4321 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
     4381 tccattttcc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
     4441 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
     4501 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagcg agggcgttac
     4561 atatttcgct acactgacat cattgaagcc catcacagaa atatcttctg gtaccactaa
     4621 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
     4681 cgcatcagga acatgcggat tcgtttttaa atattgcttc actgcttgat atcctgactc
     4741 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
     4801 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
     4861 tccagcaagc atagcgatat gttgcgcttt atgggagaca aagtgttcaa tgactttttg
     4921 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
     4981 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
     5041 tcctagtgcg atcgttccat ctgccggaag atcactaatg gaatcccacg tttctttcaa
     5101 taaagtaacg tttgcttctt ctgccttttt ttcgattcct aggcgaatag ataaataata
     5161 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
     5221 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
     5281 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
     5341 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
     5401 cttttttacg tttataaaat cgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
     5461 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
     5521 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
     5581 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
     5641 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
     5701 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
     5761 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
     5821 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
     5881 actgtttcat ccgaatatgc tttccatttt tccaatactg agttagctgc ttcaataagc
     5941 aagtttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
     6001 atgacttgcg gataatcttc tagtttgata agtttttcta tcggtgcttt tgccatagga
     6061 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaattggt
     6121 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
     6181 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
     6241 tcattgtaat aagcataagg ggaatattgg aatccccagc tttcaccgtc tagattcatt
     6301 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
     6361 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
     6421 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
     6481 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
     6541 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
     6601 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
     6661 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
     6721 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
     6781 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
     6841 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
     6901 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
     6961 taatcgcttt gatatctgtg tattcaggtt tccagcccag aatctctctc gctttttcac
     7021 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
     7081 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
     7141 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
     7201 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
     7261 cataatcgtc tccataaaca gccaaggctt ttctttgacc aagagctact tgtaaaatga
     7321 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
     7381 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
     7441 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
     7501 tatcttctgt gattggagac tctttcggct ctccataagt tgctgcagta gaagagaata
     7561 cgattttatt cacgttgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
     7621 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
     7681 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
     7741 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
     7801 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
     7861 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
     7921 aaattgtgtt tgttgattct atataattta aaaaacgaag tttactttta tggt
//
LOCUS       DAYYOA010000117         7857 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000117 DAYYOA010000000
VERSION     DAYYOA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.118"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACHBKA_002363"
     CDS             complement(21..995)
                     /locus_tag="ACHBKA_002363"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9446022.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACHBKA_002364"
     CDS             complement(1075..1950)
                     /locus_tag="ACHBKA_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM9446023.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACHBKA_002365"
     CDS             2080..3987
                     /locus_tag="ACHBKA_002365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM9446024.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ACHBKA_002366"
     CDS             complement(4528..7806)
                     /locus_tag="ACHBKA_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM9446025.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2215 a         1662 c         1462 g         2518 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttaccta
//
LOCUS       DAYYOA010000118         7841 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000118 DAYYOA010000000
VERSION     DAYYOA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.119"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..1268)
                     /gene="nagA"
                     /locus_tag="ACHBKA_002367"
     CDS             complement(120..1268)
                     /gene="nagA"
                     /locus_tag="ACHBKA_002367"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM9446026.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1344..2180)
                     /locus_tag="ACHBKA_002368"
     CDS             complement(1344..2180)
                     /locus_tag="ACHBKA_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9446027.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2364..3581)
                     /locus_tag="ACHBKA_002369"
     CDS             complement(2364..3581)
                     /locus_tag="ACHBKA_002369"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9446028.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3730..3891)
                     /locus_tag="ACHBKA_002370"
     CDS             complement(3730..3891)
                     /locus_tag="ACHBKA_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446029.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3964..4047
                     /locus_tag="ACHBKA_002371"
     tRNA            3964..4047
                     /locus_tag="ACHBKA_002371"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3998..4000,aa:Leu,seq:gag)
     gene            complement(4287..5657)
                     /locus_tag="ACHBKA_002372"
     CDS             complement(4287..5657)
                     /locus_tag="ACHBKA_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM9446030.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            5750..6099
                     /locus_tag="ACHBKA_002373"
                     /pseudo
     CDS             5750..6099
                     /locus_tag="ACHBKA_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(6371..6661)
                     /locus_tag="ACHBKA_002374"
     CDS             complement(6371..6661)
                     /locus_tag="ACHBKA_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446031.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6869..7561)
                     /locus_tag="ACHBKA_002375"
     CDS             complement(6869..7561)
                     /locus_tag="ACHBKA_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9446032.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         2407 a         1543 c         1197 g         2694 t
ORIGIN      
        1 ttgaacaata ggaaattaaa gaaacttttg aaacttatgt aaagtgattt ttactagaac
       61 acaaaaaaac cttaaaacca aacttaagaa gtttagtttt aaggttaact taaattatat
      121 tatgcgtgga aacgttccac gccatctaga taagttgctt ccaatgtcat atcagggttc
      181 aatacgataa agtctgcatc gcgtccttga gcgatttttc cacatgtatc atcgatttta
      241 cagctgattg ctggaatcag actagccatc atgactgctt gttctggtgt tgctaatccc
      301 cagtctacaa catttttaat tgcttctttc aatttcaata tacttccagc taagttgcca
      361 gattctagac gtgctgttcc ttcttttact actactggaa attcgcctaa attgtaattt
      421 ccatcaggca tcccaccggc catcatacag tctgtaatta atgcgacatg atcatgtcct
      481 gctttttcca ttaggattcc cgcagcattt ggatgtacat gatggccatc acaaatcaat
      541 tcagaaaaga catgttctaa agataataac gcacccacca tacctggttc gcgatgattc
      601 aatccacgca taccattgaa agcatggaca aatacacttg cgcctgcttc aacagctttt
      661 tgcgcttctt ctagtgtagc attactgtgt cctagagaaa caactactcc ttcttcagtc
      721 acttgtttga caaactcttc tacaccttga cgttccggtg ctaaagcaat ttttttgatt
      781 aaaccacctg aagcttcttg ccattcatgg aaagtatcca aatcaggatc cccgaaataa
      841 cttgggtttt gtgccccttt gtgttcttct gtgaagaaag gaccttcaaa atagattcct
      901 ttgattttag ctcctggtgc ttcctgatac attttgccga ttgtctcagc tacatctttt
      961 aaacgttctt tgcttgacgt caatgttgtt ggtaaaaatg aagtcactcc acaagataac
     1021 agaccttcag acatcacttt gatgccttct gcatcattat ccattacatc atgattcata
     1081 tagccgtgaa tatgtgtatc taccaaacca ggagcaatcc atttgccgct ttggtcaatg
     1141 atgttgactt ctccctcagg tagttctttg gtgtattcac caaaaagtcc atcggtaatt
     1201 tcaagatagc ctgcatgttt gacacctgac gctaaaaaga atttgtcagc atagataaaa
     1261 gttctcattc tattctctcc ctttgttttt ctacctctaa agtactctta tttagagaag
     1321 aagtcaagaa tggtctacac catttacaaa ttttttgaaa tttgtttcac agcttgtgct
     1381 aagccttcat agaatgctgc tctttctggt tcttctttac gcttgtatat taagctttta
     1441 agcacaaata aatcattgag gtaatagtag ctattcttct cttgagccca atagattcct
     1501 tgattcacct gttctaacgc ttcgtcgatt tcattttgat ggaataagaa atttccataa
     1561 ttataaaaaa tttgcgaaaa ttcacttttt gatcgttcat cagctgattg atgcagcagt
     1621 tggatgcttc gactcaaata gtagcgtgct tcagccgttt ttcctgccac accatatatt
     1681 tttccgatac aactaatcag ttgtatttct gtgtcagaaa catggattct ttctttttgg
     1741 ctagagtagc ttaatgcttc tttcaaataa taaagggact gagagatatt ttgacgcaac
     1801 gtgaattcgc aaacaccgag ataataataa tagcgttgaa agtcagttgc ttgatacaga
     1861 ttttcagaaa tatcagaaga ttttagtact tgttctaact tccgataatc acgtttttgg
     1921 aaacaactat ccaacagttc aaataattta tcgtttgtcc gagtagtttt tcgagagaga
     1981 ttcatcacat gatcaatcgt tacccctagc cgttgacaaa tctgcatcat caccaataca
     2041 ttcggcagtt cctcattatt ttcgatccga cttaatacac tttgtgaaca aatatcatgc
     2101 gaaagcattt tttgcgtcat tttttgttct tttcgaatct ctttaatgat cgttccaaag
     2161 gactcgatta acgtttccat aagtatcctc cccaaaatat gcgtattcgc attctattcg
     2221 tattttactt taggaagtcg caaacttcaa gaaaagactt gcaactaaat aatatacttt
     2281 agttatttaa gaaaactcca ctagaaagag agcctcagtg tgtttgtctg tggccctcct
     2341 actgaaaaat ttggttttta aaatcatttt tggcgatagg ttgctaggaa ttctctcatt
     2401 ttcttagctg tttgtttcaa atcatccatt ttaggaagat aaacgattcg aaaatgatct
     2461 ggctggttcc aattaaatcc accaccatgt acaagtagaa tatgatgttc atgtaaaaag
     2521 tccaaaacga atttttcatc atccaaaata ttgaatcgct ttgtatcaat ttttgggaat
     2581 atataaaaag cagcttttgg ttttacggct gaaagaccag gaatatcatt gattgcatta
     2641 tagatatatt ctctttgttc atagatcctt ccgcctggca ggagcagctc atcgacactt
     2701 tgataaccgc ctagcgcggt ttggataatt tgctgagaca atacattgga acacaaacgc
     2761 atggaggaca acatattcag cccttctata tagcctttga cgtgtgactt atctccactt
     2821 aggaccatcc agcctactcg aaatcctgct acacgatgag atttggatag accgttcagc
     2881 gtaacaacga acaaatcagg cgctaatgta gcaatcggaa tatgttctag accatccatc
     2941 actaagcgat catagatttc gtcggaataa ataatcaggt tattttgtcg agctacttca
     3001 acgatttctt ctaataattc ttttggataa agtgccccag taggattatt aggattaatg
     3061 ataacaatag cttttgtccg actcgttatt tttgatttga tatcatcaat atcaggatac
     3121 cattctgcct cttcatcgca aatataatga acaggtgtgc ctccagctaa tgcaatagaa
     3181 gccgtccaca acggatagtc cggcattgga accagtactt catctccatt gtccagcaac
     3241 ccctgcatac acatcgtgat caattcgctc actccgttgc cggtgtagat atcattgatc
     3301 gtcacattcg gaaaatgttt cagttggcaa tattgctcaa ttgcttttct agcagaaaag
     3361 atccccttgg aatctgagta gccttctgag tcacgggcat tcatgatcaa atcacgaatg
     3421 acttcgtttg gtgcttcaaa tccaaaaggt gcgggatttc ccgtgttcaa ttttaaaata
     3481 ctaatgccct cttcttgcat ccgctccgct tcttctagaa cgggaccgcg aacatcgtaa
     3541 ctgacacctt ctaatttgtt ggatttttca aattctctca tcacttagcc aatccctcat
     3601 ttcaaaaagt tagaactttc caaatttttt gttatttact atttcaatat catactccat
     3661 cctagaaaat tttgccactt atttaggaaa aacgtaagta ttaaaaaaac acaaacaagc
     3721 gttgagaact taattgtcat gatagtcatt ccatagattc gctgcccaat ccattgcaat
     3781 tccacaaatg attcctagca taaaaccaag caaaaaacgt ccgaatgcaa gtccaatcaa
     3841 tacacctaag atcattccca gtcctgaaaa ttctccgtca tatttctcca tcttaataag
     3901 tcctcccatt tttgagcttg acaactatgt aaaacctcgc tatcattagt gtgttattat
     3961 tacgcagatg tgttggaatt ggcagacaag catgattgag ggtcatgtga gcgttagctc
     4021 gtgtgggttc gagtcccatc atctgcacta caactcgtga agttaatttt gcttcacgag
     4081 tttttttgtt tgaacgtttc tttatgccat tatatacata tcactgtatc ctttacaaaa
     4141 agaacgtaca atcatgtaaa cagcctgagg tgagtatatg aaggtcggaa acaagctact
     4201 cgtagaaact tgcaagtgtt aaaacaatta atcgaaaaac gagtaaaatc cctcacctaa
     4261 tactaaagcg agggattttt tgtatattat ctgtttatca ccgttattat aaaggtacaa
     4321 ataccaagca cggttaatcc aaattgcata aattcatttt tttgtacact catgcttttc
     4381 attagctctt taactgccat tagtactaag atggcgataa caacataaag tatatagtac
     4441 gtccgcattc cggaagccca tactgtggga gaaaatccca taattaacct tgaacaaaat
     4501 cccattatca tcaaaataac taaaaaaata gaagttttct tattgtcaaa acttaaataa
     4561 attccaacaa ttaaacatag taataatgcc aagactacta aagtagctat ccaagtacct
     4621 ggataaaaaa gtgacagttt cgttcctaac tctgtaaaga gattattcag gttactacta
     4681 ctccaaatca ttgccctttt atttccattg acgtaagtaa agctttgacc cattgtatta
     4741 ccaaaataaa taatcaggtt taagaaaaac ggtaatgcag tcaatatacg cacataatag
     4801 ttttgacatt tcctatatgt tagaaggaaa attaagaaaa atagcaataa aaataagata
     4861 ttcatgtcta aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga
     4921 aagttgaaat ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca
     4981 ttccctggag ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat
     5041 aacttataat tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca
     5101 aaaaaacagg cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc
     5161 caatatattt ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt
     5221 agtaaactaa atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc
     5281 cccattactg tcaatggtat taaacagtat atagagcaaa ccaataataa atttttaaca
     5341 tttattttct caaaaacata cttgcaaagt aatactacaa ttcctaacat aatcgtagaa
     5401 tttaataatt tccatagcat aaagtgttta gaaaagaaca ttaagaatag ttctataata
     5461 attcttgaac tccatgtctt ataatgttct atcgaagcac ccaaatagcc aagttcatct
     5521 aatctacttg cataaactac gtcatccgct ctcatagtcg gcatatacat attaaagagt
     5581 aaaaagatac cccaaatccc tattagtaac aagatcagtt ctttgttatt cgttattttt
     5641 ctattatgtt tgttcatttt tatactcctt cttatatttc ctaatagagt ataacatagc
     5701 tttaagcatt acttaaagat aacttaaaag ttatctttta aatacttaat tgttaaatac
     5761 cttatttgat gcaaaagaaa tctacgatag tcaacaaaaa aattttcatt atatccagaa
     5821 aaatttttct tagttaagga cctatggatt gcagtaatgc agaattcgtc gaataaactg
     5881 ccaaagacat acaatcatat tgcttttaaa attgacgaac aagatataga ttcgtttgtc
     5941 tcaaaaatcc aaatgttagg tttaactgtc gaaccaggac gttctcgtgt gaaaggcgag
     6001 gcttcttcaa tttactttta cgactatgat aatcacctct tcgagttaca taccggtact
     6061 ctccaggaac gtttaaaatc ctacaattcc actacctgag actagtaatg actagtctct
     6121 ttttatggcc tctgatcccc ccattgagcg aatactaata gggcttcgaa ttgatctgtt
     6181 caatctttaa gcaatatccc tcaaatgagt ttggaaagtt tagtgattca tcacaaatac
     6241 ttaaatacat gtagttatat atagattgag gttcttgtga gcgtatgctc gtgtgggttc
     6301 aaatcccatc gtctgcatcc atctttccaa aacaagcggc taggacattt ttgtctcagc
     6361 cgcttgtttt ctagtttatt ctttcatctt gtcggacctt ttttcttggg tatagacttt
     6421 tctcatattc taccacaatt ttgggtttcc ctaacacatg ataggatctc acaacaaatt
     6481 gctttctatt attataataa cctgtaacga ctaacttcat cccttcgtct acttcagcta
     6541 ggaagttcaa actatgggaa gcaatcaaac aattgacccc atttaaactg aaacggatca
     6601 acggtctggc actaacttgc aaagtacgga tcttatctac tgttccatat tttttctcca
     6661 taaccttcac ctcaatacca ttatacgaac atacattccc tttgtaaaga agaaactctg
     6721 ttttatcttt ttgacttttt gtctattacc tttcttaaaa tcatattggc acttcctgcg
     6781 aagatgaagg acatgtacta aaaaaaagag gcagcagtat agattactgt tgcttcttta
     6841 ctaatttagg tttacttgtt aagaaagatt atttacgcaa atgttcttca acaatttgct
     6901 gagatttatc agctgtatct tccacttcat cttttgcttt ttctaacttt tgaatatatt
     6961 gtttcaagct tgagttttca gcagttgctt cgtctaattt cttttgtgta tctgttaact
     7021 gagtttctac atttgttttt tcttgtgtca gtgttgcgat ttgttgattc aaaccattta
     7081 cagtattttg taattctgaa agttttgctt gtgcttcttg tagtcttgct gtccaatctg
     7141 cattcagatg attgatttca tcattttttt ggttgatctc atctagtttg acattgattt
     7201 cattttgttt gctttgtagt tgtgcatcct tgctgactaa ttgactttct aagttttgtt
     7261 tttctgctgc cgattgattt ttttggtttt ctaattcatt tacttttgtt tggtattctt
     7321 ggacatctgc ctttgaatct tgcaatgtct gttctttttg tgctagtcga gcggtaagat
     7381 ctgtaagcgc ttgctcttta tctgtcaatg tatgatcaag ctgtgtcaga ttctcattga
     7441 ttgtatcaat atctgtctct ccaccccaca aaataatttc atttgcgtac cgcgtgcctg
     7501 tgtagccaat tagacataga aatgcaacga caatcccacc agtaataaat ttctttttca
     7561 tttgcatagc tccttaatcc aatactttta catgctaaaa gtaagtgatc tcaaacacaa
     7621 tgtcaagcgt ttttaaatta catgtgtaat tttaatataa atactatcaa atactcattt
     7681 aatcctcctt ttttatctat ttaaaaaatc ttttttatag agaaaaatgc aggttaatac
     7741 tttcttaaat aaatatttat ggtgaacaaa aacaaaaaag caagttttat taaacgtaac
     7801 ccttttcatt tcatagatta ctgtatttat tacaaaaaac g
//
LOCUS       DAYYOA010000119         7832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000119 DAYYOA010000000
VERSION     DAYYOA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.120"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..391
                     /locus_tag="ACHBKA_002376"
     CDS             <1..391
                     /locus_tag="ACHBKA_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446033.1"
                     /translation="LLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLW
                     LLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFI
                     GILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(517..1194)
                     /gene="nth"
                     /locus_tag="ACHBKA_002377"
     CDS             complement(517..1194)
                     /gene="nth"
                     /locus_tag="ACHBKA_002377"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM9446034.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1201..1899)
                     /locus_tag="ACHBKA_002378"
     CDS             complement(1201..1899)
                     /locus_tag="ACHBKA_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9446035.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2167..3351
                     /locus_tag="ACHBKA_002379"
     CDS             2167..3351
                     /locus_tag="ACHBKA_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM9446036.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3357..3800)
                     /locus_tag="ACHBKA_002380"
     CDS             complement(3357..3800)
                     /locus_tag="ACHBKA_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9446037.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4026..4313)
                     /locus_tag="ACHBKA_002381"
     CDS             complement(4026..4313)
                     /locus_tag="ACHBKA_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9446038.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4484..5836)
                     /locus_tag="ACHBKA_002382"
     CDS             complement(4484..5836)
                     /locus_tag="ACHBKA_002382"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM9446039.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6109..6555)
                     /locus_tag="ACHBKA_002383"
     CDS             complement(6109..6555)
                     /locus_tag="ACHBKA_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9446040.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6791..7717)
                     /gene="cysK"
                     /locus_tag="ACHBKA_002384"
     CDS             complement(6791..7717)
                     /gene="cysK"
                     /locus_tag="ACHBKA_002384"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM9446041.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2118 a         1590 c         1272 g         2852 t
ORIGIN      
        1 tttattatta aataaattta ctttaattgc tcctatcctt gcatttatta ttttttctgt
       61 acttttaagc actactctta aaggaaaatt tatggaaaga agctctcatg caatgattct
      121 cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc tgaaaccgcc
      181 caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac ttatcatttt
      241 tattacacct ttagatagat acgtcactta tttatataat cctttggaat actggactta
      301 ttttataggt atattggaag gcattatttt ctacatcggg tattttccca ataaaaataa
      361 caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta ctgagtatgt
      421 acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata ataacaagat
      481 aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt tcttttttag
      541 cgccttttct tttagacgca tccgattttt cccatcttga catattgata gcaaaggaca
      601 tacctcgcac ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa tcagtgtatg
      661 atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt ctacttccat
      721 gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca cgtgggtatc
      781 tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg tctttctgcc
      841 tacaccagga agagacatca attcttctct tgacgttggc acttgcccat cgaaacgttc
      901 aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca aaccaatcgt
      961 ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg gtgtcggaaa
     1021 agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct gggcacttaa
     1081 gatcactgca atcaataact caaatggatt attgtgcttg agttctccat gtgcttcggg
     1141 aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta acatattccg
     1201 ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt gagctgactg
     1261 ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt ccacttgttg
     1321 ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt aattaaaact
     1381 ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg gctgataatg
     1441 gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg ggcgtccaaa
     1501 ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt tttcttcgta
     1561 ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt aaagattata
     1621 tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc catttgttac
     1681 gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca tagcaatcat
     1741 ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta gaacaaataa
     1801 aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat tagatatgac
     1861 tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc cgctcaatca
     1921 cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt tacttcactg
     1981 atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt ttgatgcgta
     2041 tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga atgttgacaa
     2101 ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc ataaaggagt
     2161 ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt ctctgtctta
     2221 ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac gctgccttat
     2281 tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt ttatgctcta
     2341 gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg gcgtcatccg
     2401 atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg tttaggaaat
     2461 tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg agaaatctcc
     2521 accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa agtttttgga
     2581 tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg gggtctatta
     2641 gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt cttaaatgcc
     2701 gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc agtagattta
     2761 agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat ttcatccgtt
     2821 attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct tcaatctatc
     2881 ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg tttaagcttc
     2941 tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg attgctcaaa
     3001 aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct cgcctatcta
     3061 ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg gattatctgt
     3121 tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc caattctgtc
     3181 tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc
     3241 gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct tccgacggta
     3301 ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata gtagcttcat
     3361 ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt gtcaaattct
     3421 tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg atggagttgc
     3481 tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta
     3541 ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt tgttagctta
     3601 ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg
     3661 attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc aaattctttt
     3721 tccatttctg gtttattacg atgaataatc tcattagcta aatccagata ttcttctatt
     3781 agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt gtttttttgt
     3841 ttcctattca acaaagataa aaatattttg taggaaaacc caatctgaaa ccaagaaaaa
     3901 aaggggttga caaagcttgt cataacccct ttttctgatt aaaagatagt aaagagacag
     3961 tcatcggtaa ttgtgatgat tttttgtcat cacttaagga tgagcggcct tttcaaaaac
     4021 tgtctttaaa gatcatcatc agtttctagc acatatggct gaccttcata tgtgtactct
     4081 gtattcaatg tgccgtcatt ttcatctgag tagatgcgtc tactgaaagg aaaacgatga
     4141 gaaacattat ctttggcgtc atttgctggc atcgtgtaat attcctcttg cgtttcattc
     4201 aactgagaga gtacttgatc tgccaactca tcaatattga tcgttaattt cttcgaagca
     4261 cttttataat cttctaaaaa tgcatcttgt tggacaaaat attctttatt catcttcttt
     4321 ctcacctttc ttttcaaaat caaataaagc tgcttgttct tttatgtaac agctttatct
     4381 ttgctgctta ttgtgacaaa aaattcgaag aaagacaact aattgtactt ttcacaaaaa
     4441 aagacagagg gtcgtgacaa aattgtgtca caaccctttt tctttattgt gcagtttcga
     4501 tttcgcctag cgctgcttgt gcaagtaggt tcaagtaatt ccatgggcgg tcaaaatgtg
     4561 gttggaagaa gaaatctgaa agtgcgagat cttctacagt cattttattt tgaacagcta
     4621 aagacatggt atttgctgat tgtgtaatgt cgtatttgga catgaactga gcacctacga
     4681 tacgattcgt tcctttttca tagactagct ccatcattac tttttcagta gttggcatga
     4741 actctggacg ataattatct tcaaatacag tcgcttggac ttctagatcg ttcaaagttg
     4801 cgctttcttt ggtcacacct gtcgaaccga tcgtccagcc aaataggtaa agtcctgaag
     4861 ttccttgagt accacgatac gccattttat gatctgtcaa gttatttccc acaagcaaac
     4921 cttgacgaac ggcatttgtt gccaaaggaa tatagttttg cgtgccactt ggattgtagt
     4981 tgactactgc agagtctcca gctgcaaaaa tgtctggatt acttgtctgc atgtattcat
     5041 tcactttgat tgccccgttt ggcagtgtat ctacttttcc ttcgatcagt tttgtatttg
     5101 gacggaagcc cacacataaa atgaccatat ctgtatcaaa tgtatctgtt ggtgtagcta
     5161 cttgtttcac ttttccttct tcatctgtga taaattcagt aacattttcg cctagcgcaa
     5221 gagtaacacc gcggtctgtc aattcttttt ctaagatatc tgtgaatggt ttatctagat
     5281 atttattcaa gatgcgatct aatccatcaa tcaatgtcac ttgttttcca gattcgacaa
     5341 aagcttctac taattcaata ccgatatatc caccaccaac aattactaca cgttttgctt
     5401 ctttcgcttg tgcaataatt tcatttgctt gattgtagtt tttgcatagg agtacatttt
     5461 tcgcatcgat tccactaatc ggtgggataa taggccatga accagttgtc atcactaatt
     5521 tatcataaga aaccgtttct gtttctcctg ttttcagatc ttttgccgtt actgttttat
     5581 tttctgtatc gacgtctgtt acatcgtgtt ccattttaac tgttgctcct aatgaagata
     5641 attcttcagg atttgagtag aacaagcctg ctgggtcctt tactactccg ccgacataaa
     5701 gtgcgatacc gcaagataag aaagaaatat tgtcatttct ttcatatact gtcacttctg
     5761 cttctggatg attttttaaa atacttttta cagctgcagt acctgcatga gtacagccaa
     5821 ttactactac tttcatttat ggtccctccg attaacattt tgtccttcga caaaactact
     5881 ataacagaaa taaaacggat acaacagttt aatgcttgtg aattcacaaa ggtttgtttg
     5941 tagtttcatt ccttttcaag cataagaacc caatatctac aagattctta ttttaataat
     6001 attcactttt atatctaaga ttgtgaataa aagacaaaaa aataaaaaag ctagcgtttt
     6061 ttttacacag ctagcttttt ttaggtataa cacttgtaga aactgttttt acatttgcgc
     6121 cttttttgct gactctttac aatccggaca gagtccatac aattccaacc gatgttcatt
     6181 aatctcaaaa tcagtcagtc tgcttgcagc catttctata tcttcaagcc ctgggtaatg
     6241 aaaatcaaca atcttaccgc agttttcaca aattacatga taatgacgct ttgaactgaa
     6301 atcaaatcgg ctagaagcat cgccgtactt catttcttga acaaatccaa tcgaagtaaa
     6361 taagcgaagg ttattgtaaa cagtcgcaac actcatattt ggaaaccgat gttcaagtgc
     6421 tttataaatc tcatcagccg ttggatggga atgatgttca atcaaatatt ctaaaaccgc
     6481 gtaccgttga ggtgtgattc gaatgttctc tttttttaac ttttcgataa cctcttcgac
     6541 taacttattt tccatgtaaa caccccacgt tgttattctc gtcttatcat actattttta
     6601 cattttaaaa tccacttaac atgaaaagaa aattcaattt tattgtattt taatgaaaaa
     6661 agacgtaaaa cagtcgcgtt cactattcta cgtctctatt caaataattt ttctcatcat
     6721 aaatttttgt gtgttctttt catctttaaa agttttttac ataaacctat ctggagaaaa
     6781 accaagtgcc ttatatctct tggtaaaggg ctgtagataa ataacgttca ccattgtcgg
     6841 gaaccacagc taataccttc ttgccttttc ctaattcttt tgcgactttt agcgcggcag
     6901 caattgcagc gccagatgag atgcctacta aaatcccttc ttttcgccca acttctctag
     6961 ctgtttccat cgcttcgtct ccactaatag aaaggacttt atcatacaca gaagtatcca
     7021 gtgttttagg aacaaaacct gtaccgattc cttggatttt gtgaggtccc gcttctttcc
     7081 cttctaaaat agcagattct gctggttcta ccccgattaa ttcgattttc ggatacacac
     7141 gttttaattc tcggccagcg ccagtgatcg ttcctcctgt accgatacct gcaacaaacg
     7201 cgtccaagcc atttactcca aatgcttgat gtatctcagg accagtcgtc ttctcatgca
     7261 caagaggatt tgcttcattt tcaaattgta atggtaaaaa gtagccgttt tcttttgcca
     7321 aacgttctgc ttctctgatt gatccagaaa taccatccgc accaggagtc aaaatcaact
     7381 ctgcgccgta ccctttcatc aataaacgtc tttcgatact catcgtctca ggcatcacga
     7441 tgattacttt atatcctttt gcaactccta ccatcgacaa tccgattcct gtatttccag
     7501 aagtaggttc aatgatagta tcacctggtt tcagtagacc atcgtgttct gctttttcta
     7561 tcatacttaa agcaatacga tcttttacac ttcctcctgg attaaaaaac tcaagcttta
     7621 caaaaacatc tgctgaatct tctggtacga ttttatttaa tttgacgatt ggcgttttgc
     7681 caatcaattc ggtgactgag ttatagatag ctgtcatctt caattcctcc ttgtctttgc
     7741 tcttcttact tatcataata caatctgttc atttatggcg agtgattcgt ctttgactag
     7801 caaaaaaaag acaaagatat accatatatg gt
//
LOCUS       DAYYOA010000120         7649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000120 DAYYOA010000000
VERSION     DAYYOA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.121"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..787)
                     /locus_tag="ACHBKA_002385"
     CDS             complement(59..787)
                     /locus_tag="ACHBKA_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9446042.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="ACHBKA_002386"
     CDS             complement(800..2080)
                     /locus_tag="ACHBKA_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9446043.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="ACHBKA_002387"
     CDS             complement(2221..2823)
                     /locus_tag="ACHBKA_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9446044.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="ACHBKA_002388"
     CDS             3068..3274
                     /locus_tag="ACHBKA_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446045.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="ACHBKA_002389"
     CDS             3586..4305
                     /locus_tag="ACHBKA_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9446046.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="ACHBKA_002390"
     CDS             complement(4386..5069)
                     /locus_tag="ACHBKA_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM9446047.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="ACHBKA_002391"
     CDS             5287..7473
                     /locus_tag="ACHBKA_002391"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM9446048.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2736 a         1291 c         1439 g         2183 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tcggaaaagg gaggtgtgac
     7621 aagaattttg tcacacctcc cttatttat
//
LOCUS       DAYYOA010000121         7601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000121 DAYYOA010000000
VERSION     DAYYOA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.122"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHBKA_002392"
     CDS             complement(138..428)
                     /locus_tag="ACHBKA_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446049.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHBKA_002393"
     CDS             complement(546..806)
                     /locus_tag="ACHBKA_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446050.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHBKA_002394"
     CDS             complement(943..1356)
                     /locus_tag="ACHBKA_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM9446051.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHBKA_002395"
     CDS             complement(1812..2297)
                     /locus_tag="ACHBKA_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446052.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHBKA_002396"
     CDS             complement(2436..2651)
                     /locus_tag="ACHBKA_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM9446053.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHBKA_002397"
     CDS             2875..3675
                     /locus_tag="ACHBKA_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9446054.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHBKA_002398"
     CDS             3694..5562
                     /locus_tag="ACHBKA_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9446055.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHBKA_002399"
     CDS             5555..6046
                     /locus_tag="ACHBKA_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM9446056.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHBKA_002400"
     CDS             6034..6588
                     /locus_tag="ACHBKA_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM9446057.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="ACHBKA_002401"
     CDS             6606..7601
                     /locus_tag="ACHBKA_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM9446058.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2709 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAYYOA010000122         7534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000122 DAYYOA010000000
VERSION     DAYYOA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.123"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHBKA_002402"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHBKA_002402"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM9446059.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACHBKA_002403"
     CDS             complement(2032..3285)
                     /locus_tag="ACHBKA_002403"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM9446060.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACHBKA_002404"
     CDS             complement(3291..4214)
                     /locus_tag="ACHBKA_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM9446061.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACHBKA_002405"
     CDS             complement(4435..6927)
                     /locus_tag="ACHBKA_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM9446062.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACHBKA_002406"
     CDS             complement(6934..7401)
                     /locus_tag="ACHBKA_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM9446063.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2075 a         1638 c         1208 g         2613 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAYYOA010000123         7460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000123 DAYYOA010000000
VERSION     DAYYOA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.124"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..1130
                     /locus_tag="ACHBKA_002407"
     CDS             102..1130
                     /locus_tag="ACHBKA_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446064.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1199..2539)
                     /locus_tag="ACHBKA_002408"
     CDS             complement(1199..2539)
                     /locus_tag="ACHBKA_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9446065.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(2698..3138)
                     /locus_tag="ACHBKA_002409"
     CDS             complement(2698..3138)
                     /locus_tag="ACHBKA_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9446066.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            3298..3804
                     /locus_tag="ACHBKA_002410"
     CDS             3298..3804
                     /locus_tag="ACHBKA_002410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9446067.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3972..5885
                     /locus_tag="ACHBKA_002411"
     CDS             3972..5885
                     /locus_tag="ACHBKA_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9446068.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5890..6891
                     /locus_tag="ACHBKA_002412"
     CDS             5890..6891
                     /locus_tag="ACHBKA_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9446069.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(7093..7299)
                     /locus_tag="ACHBKA_002413"
     CDS             complement(7093..7299)
                     /locus_tag="ACHBKA_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM9446070.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2600 a         1202 c         1528 g         2130 t
ORIGIN      
        1 aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga
       61 tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg
      121 acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat
      181 ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa
      241 gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga
      301 acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt
      361 tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt
      421 attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata
      481 aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact
      541 tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc
      601 atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag
      661 aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta
      721 gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga
      781 tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat
      841 tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat
      901 ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca
      961 ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact
     1021 ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca
     1081 ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcggc
     1141 tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata atcgagatac tcacacggct
     1201 agacagctat cttgtctagc cgtgttgttg ttctggcttt agtcagttgg gtagtgttct
     1261 tagttttctt gaaatatttt cctcgatata aaaagattcg ttctcggtag ttttgaaagt
     1321 ttctaaagcc ataggcaatt cgttttagta ctttgatatg gttgttcaaa cattctatag
     1381 ggccgttgga atagggaact cgcaacgctc gtttgatttc tgttcgatat ttcttgaagg
     1441 tcgtgaacac tggttgatag tgctctggca actccttgta gtcttctttt aggagcgcga
     1501 cgaattcagg aacatcttgt cttctgatag cggaaagaaa aacctggtag acttcgtagc
     1561 ctcgctttaa ggagtcatca taatataata gccgatcgac tatctccgct tctgtcagat
     1621 gatctcgaaa tgacggacgc cagtagcgat gctgaccatt caattcccac gcattctttt
     1681 gaagtagtct ccagtatttc tttaagtgct tcgaccgacg atcccctttt ttaaaggtct
     1741 tcattacttg aattcggtgg ttggtaaagg cccgaccgat gtgttggcta atatgaaagc
     1801 gatcggcgac gattcgggca ttcggaaaac actctttcac caatgtgcga tagggtgtat
     1861 agaaatctga aaccactaga cgtacttgtt cacggacttt tctagggtag cgcaagaaat
     1921 agtcgcgtaa aggattgagt tgccgatttt ccacaatatc aatcagttga tgggtcttgc
     1981 cgtccatcat gacaaagctc atggctcccg agacgttttt caccgatttg aattcatcga
     2041 aacacaccac ttctggtaac ggctggttca aagaaacggt tttaggccga aggctgcgaa
     2101 gtattcgaat gacagtggtc ggagaaatgt gcttcatacg tgcaatcaaa gacatagaaa
     2161 taggctcact taatagctca agaatggctt gtttgactct gcgggcaatg gagcagcgac
     2221 gatcgctaac tgaatcttct gctaaaaaag tctttccaca gtgtttacat ttgaagcgct
     2281 gtttgttaat ccgcacaatg gtcttgtagt tactgacatc attcaataag atggatactt
     2341 ttttccagcc ccatttaatg atcgtttgct tattttgata gtggcaatgg aagcattcct
     2401 ctggttgata ggtcaatgtg ccatcaaaaa caaaacatgt ttcgtcatta atgcgctctt
     2461 tgcgaaaggc atcttcatga aaggtaagat ttaggtctag aatatctagt aactctttgg
     2521 taaactctga ataggacatc ttgaaacact cctttgaatg gttttcgtcg actttattct
     2581 aaaggatttc aaggtgtctg tttgcgttta ttagaaaaaa gaaaaagtat tggcaacaga
     2641 aggcgtcacc aacactagat attatagatc cagaaaagca gattgtttat gccaaaatta
     2701 agacattgca aaaagattat tttctttaaa atcgacaaga tgaccagaaa gcaagagttc
     2761 gttgagctct tgctttcttt tagttttaga aaggaacatc ctgattttat cttcgctaaa
     2821 tccagtcaaa agttttgcag attggtcgta gataatcggt tttttcaata aagaagggga
     2881 tgtacgaatc tcaatatata gttcactcaa actgagttca tctattctgt gaacaagatt
     2941 tttataacta atagaatgag tagagagcat ttcggtcacg ccgttgtttg tcaattttag
     3001 taagtggata aattcatttt tggttagtgg ttgacgaatt aagttctgtt cgataaacgg
     3061 tatatcatgg acgataagcc aacgttttgc gcgtcttgaa gacgagcagc cagaacctgt
     3121 atacaattta atcatcatac attcctccaa tagctaagta tttatgaaat aataacatga
     3181 agaaatagtc gaaaagtgcc atttagaggt gtaaaataag aaaatgttgt cacgtataat
     3241 ggaactagct agataagaaa tactattaaa tcaagaaaaa agaaagaagg cacaagtatg
     3301 tcaaacacag ccgacgcaat agtagtgatc gatttacaaa aaggggtatg ttttgattct
     3361 tcgccgatac acgaattaga aacagtgaca gcattagtga ataaaagaat caaaacatac
     3421 gaaacgattg ggaaaccagt tattttcgtt caacacgaag atgctcaatt agtgccacaa
     3481 acaaaaccat gggagctatt gtcggaatta tctattcctg agcacagtta ttttgtgaga
     3541 aaaacccatg cgaattcatt ttataagact aatttgagtg ttttattaac tgaattggac
     3601 tgtcattcga tcgaattatg tggggcacaa gcagagtatt gtatagatac gactgtgaag
     3661 tttgctcatg gattaggata ccagcttatg atgtgtagag gagcaacgac aacttatgat
     3721 aatcctttta tgaaagcaaa ggaaacaatc gctttttacg agaaaatttg ggataaacga
     3781 tttctgacat ttttggagca ataagttgtt tcttcgttta aaaaaagcag aatggatact
     3841 ggtttatcca ttctgcttta ttacgtaaaa tgagaaaata atcctgttct atttttatgg
     3901 gataaaccat ttattttttg tataaaaaat gtttgcgttt acatataatt ctcctagaaa
     3961 cataaaaagg agtgaatagc ttgagaacaa aacaagtatt aaagggagcg ttggtcatct
     4021 tatttgctgt aagtacaagc gtcccgcaat ggacagtata tggagagagc atttcttccg
     4081 aatcggaatc tcgggaggta gaaaccacag aaactactga tgctggaaat gaaacgaaat
     4141 cgagtacttc acaaacagaa acaaatactg aaacaacggt tgagacatct gttactagca
     4201 cagatcaaga aacgaccgct accacagata accaacaaac agaagaagca acagctacaa
     4261 aaatgtcaaa cgacgaatcc tcttctgcta aagtaagtga caaagagaat gggacagaaa
     4321 atgatgatat cggtaaaggt gaaaatttca atggtaaaga atatttgaat tacttcggta
     4381 aacagatgac agaaatactt aatggaccaa cagaagacat caagtcaaaa ttaatagagt
     4441 tattagtcac tgagcttgct gttaatgatt atggtgtaga taaagagatt atcaaacatc
     4501 tgattgctac ttatctattg tacgatatcg cttttgcaaa aaacagtgtg ttgatgtcga
     4561 tgcttggagg tacagatgat gggacaacgc ctacagaaaa cacaggcttc tatccaaata
     4621 caaaacaact tcatcaaact gtactaaaca aacaagatgg caaaatgatg gatgtatatg
     4681 catattatgt cgatcaaggt tcagataaaa cagtcatgat ccatggcgga tttcgaggga
     4741 attggaataa cgggatcgtt acagaagaat acaatgattt ttataaggcc gggtataatt
     4801 tattatttgt tgattccaga gcgactggta atagcggagg cgattatgtg acctatggac
     4861 aatatgagtc agatgacgtc ctttactgga taaatcaaga agttcgagag cggccatctc
     4921 aaaagatttt actttatggt ggttcgatgg gagcagctac gatgatgtct gtattagcga
     4981 aagatattcc tgtcaatgtc aaaggaatta ttgaaaattg cggatttgca tcaattgatg
     5041 aacaactacg ttttacgtac agccagacag tagcccccgc actaccggat gccataaaaa
     5101 atcagttaga catcattggg gatcaagagc atgaagattt atttatgggc cttttgaaac
     5161 aatactattt tgatcaggag atgcatttgg atacaaaagc tgctctacct acgattggaa
     5221 tgtcagattc attgcctaaa ttgattatcc atggaacagc agatgatgta gtacctgtgt
     5281 ctaacgcgca aaaattatat gagttatcag gagggtataa agatctttta ttagtagaag
     5341 gagcagggca tggaaaggca caggaagttg accatgctgc ctacacgaaa catgtaacgg
     5401 actttttgaa agtagtgttc catgatcaga taaacgtaaa gtatgtggat gaaaacaata
     5461 aaagtctact caaagaccag gacgaaatcc agctttacgg agcttatgga gaaaattata
     5521 tgacagagca aaagacattc gaaggatacg aattagcaaa tgttgaagga ccgactgaag
     5581 ggatttttaa tgagacgata ccaactatta ttttctatta caaaaaaatc ccagtagctc
     5641 caccaaaaaa gcaagatccg acagagaatg cagacaataa gggaaagaat gaacctcgag
     5701 aggatcaatc aagtgatgat acgcaaccga ttcagatgac agtgaaagta caaaaaaaga
     5761 cagatgcaaa aaaaagaatg ttgccgaaaa ctggagaaaa gaaacattcg cttctcattt
     5821 attcaggtgg atttttatct gttagcacga ttatcacatg gatttggcga agaaagagag
     5881 ggtaaatgaa tgaaatggtt caagcgaata gtgattgcag taccagttat catcgtagtt
     5941 attttagtca ctgtattttt gattaatcaa atcacaccaa agccgattag cttgcttgtt
     6001 agaaaacaat ttgacacaag tgaaaaagaa caagtatatg cccgaccaga tgatttccag
     6061 caaaaaatcg aacaaatcga tataaaaaaa gaaatatcct atccttcaaa gtacaaaaat
     6121 aataatatgg atatctacac accgaaagtg gaaaagaaaa aattaccgat tcttttctgg
     6181 atgcatggag gcgcatatgt aggaggggat aaaaatgatt gtcgtgatta tttggaatat
     6241 ctatgttcag acacgcagca aatcatcgta aatatagatt atgaacgatc accggaagca
     6301 aaacatccta caccagttat ccaattgaac gaagccattc aagcagctaa aaaagagata
     6361 aaagatcaag cagattggtc gaatatttcg attggtggaa attcggcagg tgcacaaatt
     6421 agtggagaat atttgttagc acttcaaaat gaagaaatta aaaaagctga tcatcttgat
     6481 ccgactttgg agaacaagca gattacgaag tttatttctt tgtctggtct gttggatcct
     6541 tctgcactta ctcaagtaag tgataaaatc tctagctttc tttatgaaaa atgtggatgg
     6601 gcttactttg ataataagga ctttgaacaa tcagagaagg tcaaaagttt agccttggtc
     6661 agacatgcgg atcgttggac gcaaaacaca ttcttaacgg atggaaatac aaatacgttt
     6721 acgaaacaaa tggaagaagt aacttctgta tttgagaaag cgggggtgaa agtcacaaaa
     6781 gtagattatg cggagaaaaa tgctgttttg aaccatgaat atcagtttga tttcagcaat
     6841 aaacaagcgc aagaaacata tcaaaagtta gtaatgtttt tcaaggagta aagtcttatg
     6901 atgcaaaaga ggttgtaatc tgtcacttag ctccaagtca caaggagcaa tttcaataaa
     6961 caagcttttc atggtttatg aatattgtaa cttgtgactg aggagttaga gatggcaagg
     7021 ggcacctttt attcgaaatt tgggaatatc acacgattat tgtcacgatc tcttttacgt
     7081 aaattttata agtcagattt gttgttttga tcgtttaaaa aaagcaacaa atccgaagcc
     7141 tgcggaggca agaagggaca aaaagccgag tggtaagcaa aagaatgctg gttccactaa
     7201 tattccgttg ttattgatat aaggctggga acaaagatga atagcaaata aaatcaatgc
     7261 actagcgaaa aaaagcaaag aaatatggat agctttcata agcagcacct ccttttttat
     7321 aattgtatga taaatagatt aagaatctat aaatgtgaca agtttgttag gatgtgaata
     7381 aaatgatcat ctagcgaact tgaaacaaag atggctaatg ataagcaatt aatgattcag
     7441 caatcagata ggtacaaagt
//
LOCUS       DAYYOA010000124         7411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000124 DAYYOA010000000
VERSION     DAYYOA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.125"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..406)
                     /locus_tag="ACHBKA_002414"
     CDS             complement(<1..406)
                     /locus_tag="ACHBKA_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM9446071.1"
                     /translation="MNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLGGKVYYAPRLY
                     PQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRIAHSHNTSIDR
                     DFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLF"
     gene            complement(466..1602)
                     /locus_tag="ACHBKA_002415"
     CDS             complement(466..1602)
                     /locus_tag="ACHBKA_002415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9446072.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1634..3085)
                     /locus_tag="ACHBKA_002416"
     CDS             complement(1634..3085)
                     /locus_tag="ACHBKA_002416"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9446073.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3129..3893)
                     /locus_tag="ACHBKA_002417"
     CDS             complement(3129..3893)
                     /locus_tag="ACHBKA_002417"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM9446074.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(3921..4619)
                     /locus_tag="ACHBKA_002418"
     CDS             complement(3921..4619)
                     /locus_tag="ACHBKA_002418"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM9446075.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4631..5410)
                     /locus_tag="ACHBKA_002419"
     CDS             complement(4631..5410)
                     /locus_tag="ACHBKA_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM9446076.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5426..6370)
                     /locus_tag="ACHBKA_002420"
     CDS             complement(5426..6370)
                     /locus_tag="ACHBKA_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9446077.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6416..7195)
                     /locus_tag="ACHBKA_002421"
     CDS             complement(6416..7195)
                     /locus_tag="ACHBKA_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446078.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2068 a         1578 c         1151 g         2614 t
ORIGIN      
        1 gaaacaaaaa atcacctgct acttttccac atgaaaattc atgtgtcaaa acagacgtta
       61 acttacatcg ataatattgc tttaaaagat atttaaaatc tctatctatc gatgtattat
      121 ggctatgtgc tatccgtatc ggaatcccag ctttttttgc agcttttaaa ggtaaataac
      181 tcatcgcatc aatatgagaa tgaacaatct catattctgg atgatcctta aaaaattgtt
      241 ccatccattt aaaatattta ggataatttt gtggataaag tctcggtgca taatagacct
      301 ttcctccaag acttattatt tcctcatcat aatcactttt atatggacga tgtgtcaaaa
      361 aatcaaactg aattttagtt cgatctatat tcctataata gttcattagc atcgtctcca
      421 accctgcacg atgcatatca ttcacacact gtaatattcg aatcattata tatttcccct
      481 atacaaattt aatgtttctt gtacgatttt atcccaatta tatttactac aaataaactc
      541 agtagcttga ttcttcaaaa ctttaaccat ttccgattga ttgcatgcct cttctaatct
      601 tatctttaaa tcacttacat ccgatttctt aaaaatcata gccttgtctt ctactacttc
      661 cgtacactct gagatatttg aaactataca acagttccca taactcattg cttctaacaa
      721 acttaatggc attccttcta aatcacttgg aagtgtatag atatacgcat tactatataa
      781 ttcttctaat tcctgtcctt gtacaaatcc agtaaatata attcgctcat caccttttgc
      841 taattccttt agttcatttg caaattcatc tgtatcagag cttcctccgg ctattataag
      901 ttttttatct gtctgaacat ctttaaatgc ctcaattaga taccgaattc ctttttcggg
      961 aaccaatcgc cccaaaaaca aaatataaga atcttttgtt aatccatact tttctgtaat
     1021 caatcctgct gattttattt caattttttt aacgccattt ggtataaacc ttgtttttct
     1081 tccatatata tcttcaaaat aattttggac gctttcacta agtacaatta tttcatctgc
     1141 aaattttaca gcacactttt cccctaacat gatgtatgta ctagcaagct tgttccattt
     1201 ttctctttga tgatctagac catgaattgt tgcaacacat cttttcccaa aaagttttgg
     1261 caaccacaac atagcacatg gtccttctgc atgaaaatga acaacatcat attttccgaa
     1321 tgctgctcta attccaccaa atactgaaga actcattgct gcaattcctt ttatatttaa
     1381 tgtaaaaata gtctttaatt ttacaccttt atattcttta tttttatgct ggtcaaattc
     1441 ctttccgcta acatgatgac cacttcgatt atagcaagtc acttcatgcc caagtgcaac
     1501 cattcgcgtt gccaattctt ctaccactat ctcaattcca ccttctcttg atggaattct
     1561 tttatgacct agcatacaga tttttaattt ttttcttgcc atcacgtttt actccttcta
     1621 tagtatcttt atcttacatg gaaccgtttc tatgaagcac agttttgact gtcctaaaca
     1681 aaattctaaa atctaatccc atactccaat tcctgatata ttctgcatca agttttacaa
     1741 cctcttcgaa gtctgtgata tcacttctac cactcacctg ccacattcca gtaatcccag
     1801 gtttcatcgc aattctcgca cgatgatgaa aatcatactt ctcatattct gacttcaagc
     1861 aaggtctaaa ccccaccagg ctcatttttc ccattaggac attataaaat tgtggaaact
     1921 catccaatga tgtagatcga ataaattgtc caattcctgt cttctttgtt ccatcaggaa
     1981 gtattttatt gccaatgatt cttgggtcaa aatccatctt aaacatgaga ttattactca
     2041 tcttattttc tttcatcaac tctgctttgc gttcttctgc atccatatac atactacgga
     2101 acttatacat cttaaatgtc ttaccatttt gaccaatacg ttcttgtgca aagaaaatcg
     2161 gccctggtga tttgatgtaa ataactggtg ctacgaagat aaaaataaca cctgttgcaa
     2221 tacatccaat aattccagca agaatgtcaa tcgttctctt tataaatgct tgcctcatcg
     2281 tcataacatt aatactagat gtaataacag tataatcacc aactcggcca ataaattgtt
     2341 ttccacgtgt actatcagac acttttgtaa gattcatatg tacagcaagt cccatttccg
     2401 aacatctggc gattaattct ttgggatatg gaacactttc gtcaacattg atgaatactt
     2461 catctaccca attctgtaaa agatattccg ctgcattatc agcatttgca actactggga
     2521 cacctccgat ggtagttcca tacatatctc tgtctactac aatcacacca ctaagtttat
     2581 acatgccata attcttctct ttaagattac gaactacatt ttttgcaatc ttttgagatg
     2641 ttacaataac taacgataca tcttcaccgt ttttcatgcg gtgatgcaac aaccctttcc
     2701 aaagtatacg aattatatat gttaataatc catacaccac ccccattata aataaagaaa
     2761 ctctggaata atcatttcca tcctgcattg taataaggta taaactgcac acaagtaaca
     2821 gcataaaaga ttgctttata gaagctacag cctctttata atatcctctt tttaaaacat
     2881 ttttatatgc ctcaaataaa aatataatcg taagattctc aagttctata aaaattgcca
     2941 tgtggcgata aataggaatc acatacggat tccagtcacc ctgtcgcatt acatatgata
     3001 tataaaaagc aagctggaca caaaataagt ctaataaaat aaaatcaata tgtttagacc
     3061 aaccacttga gttttttcta tacatctctc actctctcct catttccttt ttatctacga
     3121 ataaagaact aaaataaacc aaacttcttc ttttttacct cttcatagat tggataattg
     3181 atctcatccc cgttgatcag atcctttgcg acttgtttca tctgtactac tttttcctta
     3241 ccaaactcct tggcaatctg ctcatagcac tcttttaagt aaaaggtgcg cttagagacc
     3301 ccgtgtgcat cagaagcagc catctgcacg agattatgtt tgaccattgt tttggctgtt
     3361 ttctggatat ctttgccaaa tacaccaaca taacttggcg cagtaagttg ggccaaacat
     3421 cccatatcta ggaacggaag caaatgattt gggtcttctc gaaaatgcgc gttgcgttct
     3481 ggatggacga tcactggtat ctttcctagt tctaacaact caaaaaaaag ccgttctgca
     3541 tataacggca cttccagtgt cggaaattcg atcagaagat atgtatcatc taaatctgta
     3601 aataaaattt catctcggtg gatgtcttcg atgagcgttc cggtaatacg tacttcttgt
     3661 ccttccaata acgtcaaagg caactgtctt ttctctaatt cggcttgtaa cgaagcgact
     3721 agagaaatga cttgtgattt ttcattgctg tattttccat tattgtggtg tggtgtgcat
     3781 aaaatatgcg taatgccttg ctgaatcgct ttttcagcca ttgcaatact tgcttctaag
     3841 ttttctgccc cgtcatcgat tcctggtagg atatggcaat gtagatcgat catttctctt
     3901 gtctcctctc ttcttattcc ttaatttccg tagtaataac cagcatcttt gctgtgttct
     3961 gctccgttgt atacgacacc taagactcgt gcttgtacca tatctaacag ttcttttgct
     4021 ttagtcaatg attcttttcg tgacgtattt tcccgtacga ccaataaggt gccatccgtt
     4081 tttgaagaca tgatctgtgc atctgttacg gcaacgactg gcggcatgtc gaagatcact
     4141 acatcaaata gttgtcttgc ttctgctaaa atctgatcca ttcttggcga acttagtagt
     4201 tctgatggat ttggtggttt aggtccactt ggcagaatcg acaaattgtc gactggtgtt
     4261 cgttggatct catctgctac acttcctgat gaactaagaa cagtacttaa tccgcttgcg
     4321 ttgttcaact tgaacgtttt atagatgacg ggcttacgta agtctgcatc gactagcagc
     4381 acccgttgac cagatttggc aaaaacaacg gcaatgtttg ctgcagtggt cgatttccct
     4441 tctccaggtc ctgatgaagt cacaacaatc gttttgattt gctgaccagc tgcggaggca
     4501 aactggatat tcgtccgaat cgtacgatat tgttcagcga ttggtgaaga gggattggtg
     4561 agtgtgacca aactgactgc atgagtttgg gctgcttttt gtttctgtgt tcgtgccatt
     4621 ttctttcctc ctagactctt gagcggcttc tgcgtgatgg cgctgtttta tcgtttgtag
     4681 cttttggttg tgtctgagct ggtccttttt tacggatacc agcattcaat tctttcgctg
     4741 acatttctgg aactgttcct aagattggga atcctaatgt atctgtcaca tatttttcat
     4801 ctttcactgt ccgatcgagt aattccaata agaatgccag acctacacct accatcattc
     4861 ctaacattaa gccagtcatt aaattcaatt tattatttgg cgaaacaggt gtcgtattgg
     4921 caactgcacc tgaaatgatc gagattttat caacattcat cacatctttt gcgttctctt
     4981 ggaaaacttg tgaagttgtg ttagcgatct gttgagcagc cactgcattc gtacttattg
     5041 cctgaatcga gaacatttgt gaattttctg cctgattaac tgagatggca tctttgattt
     5101 gttcagcagt cattttcaca ttgtcttctg tttctaaacg atctttgact tcattgagca
     5161 ctaaatcact gacgatcata tctttatacg tattgatcat ttgcaggttc gtattgacat
     5221 cattgacatt tgcagtgtct gattgtggca gtgtcacgat caattgtgct tgtgaactgt
     5281 attttggtgt caagatgaaa aatgttacga ttcctgagat ggccaagcca gcaaacattg
     5341 agatgatgat tattaccaag tgtttgcgta atgtttcaaa aatttctttc aagctgattg
     5401 tctcttccat tttttctcca ctttcctatt tattctctag attcatttga tcttttaatt
     5461 cttttttcac acgatcaagt tcttgatcac tgactttttg ataggagata ttatcttgca
     5521 tgaagccttc tccttgcagc tgatcttgct taatcgtacc aaatgcctcc cgataatcta
     5581 aagcaatttt tttcatttca tcaaagttca tgtctgtttt catattcgac tctagagcat
     5641 tcaaaacgga ttgatagttg ctgacaccgt ttagactcaa tacttttttc gctacgcctt
     5701 ctacgatctt tctttggcgc ccttgtctac cgtaatcacc atttgggtct tcataacgca
     5761 tacgagaata agataatgcc tgatccccgt tcaatgtaat tttaccgatt ggaaagtcat
     5821 agccatcttg tgagaaagtg aggtcattat tgacttcgat accaccaact gcatcaacga
     5881 gttcctttag ccctttcata ttgatagaga catagtgatc gattgggatg tctacatatt
     5941 tttgtacggt atccatcgcc atggcagctc cgccgaatgc gtaagcgtgg ttgatcttgt
     6001 cttttgtgtc acgacctacg atgtcgacat acgtatcacg aggaatgctg acgatcgttg
     6061 tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt atctgatctt ccttgatcga
     6121 cccgacctaa atctccagta tcaatcccca tcaatagaac agagaaagat tcttgtttgt
     6181 ccagatcaac tggtttcgtt cgttctcctt gggttcgttc cactgattcg taagttttct
     6241 caatagaatg tgagagatcc agatataatt ttgctcctac tcctacaatt gctaatacta
     6301 atacgataag aactgctaaa ataatcagaa atattttttt ccctcttgat tttcttcttc
     6361 gtcttgccat agtttcctcc gtttgctaac cttgcttgaa tcaacgtttt aactgtcaat
     6421 agcttatatt gctatagccc atttgctcag caacagtttg tgcttttgtt gcgtatgctg
     6481 cgcttatttt ttcattccgg atctgctcta tttgactgag caccgtcaaa tattgatcgt
     6541 aagtagcggt ggcagtcact tgaccatcct ttaacatcgt atcgaataac tgatctagtt
     6601 gcgtgacttg cgcgatctga atatcagcga atcctagata ttgcataaca accgttttcc
     6661 aaggactatc tttaaaaaag ctcatatttt cacgtacacg agcgacatct gcacttgtca
     6721 ccttttcttt gatgatgaca tcgtctacag cttgctgcca gtttgataca tttttagtaa
     6781 ataaatcgtt gacagctgtc tggatcttca gttttgattc tgcactatct aactgtgtca
     6841 cgattgcttt cttctcatct tggattgcaa cagcttcttt aggcaattct gattctttga
     6901 tgtcgtaatc atctgctgaa agtttgacag aggatagctt atttgacaaa cttgtgattg
     6961 tttcttctgt tatttcaggg tttagaaaaa ttttttcttt tgtactatac agcttgtcta
     7021 cttcttttga taaagaagtc aaggtgtctt tttgattggt gatggcagat tctgcataat
     7081 tgatccgttg ctgatgttgc cgctgattat tgatatggta tccaaacaaa cagatcgcaa
     7141 tagcacctgc acctaccgcc acagcagtca atgacttgac gaatggcttt tccatagatt
     7201 cactcctaag tattgatgat agataaatta agatataatt cacttacata aaaatgacag
     7261 ctttttattt aaaaaaacat aattaaacta atatattcac agaaatattt gtaaacaagt
     7321 tttccctata taatcatacc acaaactgtt ttcttgacta gggcaattta cgatttaaaa
     7381 gtaaataaaa cattaataag ataaaaatta a
//
LOCUS       DAYYOA010000125         7397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000125 DAYYOA010000000
VERSION     DAYYOA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.126"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..991
                     /locus_tag="ACHBKA_002422"
     CDS             <1..991
                     /locus_tag="ACHBKA_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFM9446079.1"
                     /translation="ISDHSKNTKEIAKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKD
                     CLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACLYRNNKNVQVSILEE
                     GILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVT
                     AGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDNL
                     IVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMSA
                     KPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            988..2409
                     /locus_tag="ACHBKA_002423"
     CDS             988..2409
                     /locus_tag="ACHBKA_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9446080.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            2445..3161
                     /locus_tag="ACHBKA_002424"
     CDS             2445..3161
                     /locus_tag="ACHBKA_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9446081.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            3180..4763
                     /locus_tag="ACHBKA_002425"
     CDS             3180..4763
                     /locus_tag="ACHBKA_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFM9446082.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            4892..5476
                     /locus_tag="ACHBKA_002426"
                     /pseudo
     CDS             4892..5476
                     /locus_tag="ACHBKA_002426"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            5422..5940
                     /locus_tag="ACHBKA_002427"
     CDS             5422..5940
                     /locus_tag="ACHBKA_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446083.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            5963..6988
                     /locus_tag="ACHBKA_002428"
     CDS             5963..6988
                     /locus_tag="ACHBKA_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM9446084.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            7237..>7397
                     /locus_tag="ACHBKA_002429"
     CDS             7237..>7397
                     /locus_tag="ACHBKA_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9446085.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2710 a          846 c         1399 g         2442 t
ORIGIN      
        1 tatttctgat cattctaaaa acactaaaga aattgcgaaa aaaatagaag aaaaaaaagt
       61 gtttgatggc tgtcattatt tagaaagcaa atcttttgat gataatattg ggtattttca
      121 aaagattaag gactgtcttc agatttcttg tggtaaatca aatcgatatg cctattattt
      181 gaaaaactgt aaaagtgaat attttgatga atttatttgt ttttcaatgg gagaggtttg
      241 gttggaagga atatatgctt gcttatatcg aaataataaa aatgtacagg tatcaatttt
      301 agaagaaggt attttaagct atggagttga cattattcca acagcaagaa caaaagctat
      361 cgcagtactc aattacttaa aaaaaaggac tccactatat aacaaaagct ataattttta
      421 ttgttttttt cctaatatat atacaggaag agagaataca atacaaattc cattagttac
      481 agctgggtca agaaccgcat tgcaactttc ggatattttt aaccctatga tagatattta
      541 taaagaaaaa aaatatatat tttttacgtc tgtttatgat tttgagggcg gagaacctgt
      601 cggagaatat gaattagtat gcaaaatagc agacttagta ggaaaagata atctaattgt
      661 taagacgcat cctagagatg taagaacaat ttataaagat aatggattta tcgaggataa
      721 aaattcatca ataccttggg aggcgattca attgtcaggc gattttaacg ataaaatatt
      781 tatgacaatt aattcaggaa gtgttatttc aggtagtaca atgtctgcaa aacctgtacg
      841 cacgtattat atgtataaat tgtgtaacat agaaggaaat ccgtcttgca aaaagaatgc
      901 gtatgatatt gagcgattgc ttaagcaaaa aacgatgaaa aacattttaa agaatgtgaa
      961 aatagcagaa agcttagatg atatactatg agcaaaacta aatatttaat taaaaatatg
     1021 ggaatattaa caataagtaa tttttcttca aaaattttag tttttttgtt agtcccttta
     1081 tatacaagtg tattatccac aacagaatat ggcacatatg atttgacaat ttcaacagcc
     1141 acattattgt ttccaatttt aaccttaaac attgtagatg ctgtgatgcg ctttttaatg
     1201 gataaagata aaaataaaaa aaatatagca tctatagcga ttaaagttat atttttaagc
     1261 gtgatagtct ttggatccgt aatgtatttg ttaaatatta caaaattatg gcaagatgtt
     1321 tatggattag aaaaatatat ttttttgtat tatatatcct atgtatggaa tcaatttcta
     1381 atacaatttg ctaaagggtt agaaaaagtt aaagctatgg gcatagcagg tattatcagc
     1441 acaattgtaa caataattgc caatattttc ttcttgctta ttctgaaatg gggattaatt
     1501 ggttttttca ttgcaaatat tttatcacag attatttcag ctacttatct gtcagtatta
     1561 gtgagaatat gggaatatat cgattttgag gctaaagatc ctcagctaaa gagagaaatg
     1621 ctaaattact cgattccatt aattgcaact gttgttggat ggtggattaa tagtacagct
     1681 gataggtatg tggtttcctt tatagtaggt gtctcggcga atggcttatt gtctgtggca
     1741 tataaaatac ctcaaataat taatactttg cagggaatat ttatacaggc ctggcaaatt
     1801 tctgcaatta aagaatatgg agataatgag actgcaaaat tttatggaaa tacatttgtt
     1861 atgattaatt tgttaatgtg cataacctgt tcaagtctta tcatattaac tagaccgttt
     1921 tcacatattc tttatgcgaa agacttttat gaagcgtgga aatatgttcc atttttatta
     1981 atagctaatg ttttaaattg tgcatcaggt ctgttaggac ctatcttatc agcaaaaaaa
     2041 gattctaaat ccatgatgtg gtctgcaatc ataggggcat tagttaatgt gattatgagt
     2101 gtagtccttg tttataagat aggggttcaa ggtgccacaa tagccactgt tatatgttca
     2161 tatgtaatat atgttgttcg taaacgagct gtgggaaaag atatttttat aaagcattat
     2221 catattgttg tggaatcttg gggattattg tttgttcagg caatcataga aatatatttt
     2281 aaaaattatg taatagaaat tataattatg attattttaa ttattataaa tatcagttat
     2341 attaagcagt tgtgttatag aggaagaaac attatttcaa tgattaagag tcgagagcac
     2401 aaacggtaga cgatgttatt tttataaaaa gatataattt attaatggtc gataattatg
     2461 aaaggaaaaa tattcttaaa aaaaagagta taggatgtcg tatgaaaata gtcgctatag
     2521 taccaatgaa gttaaataac aaaagattgc cacaaaaaaa cacgaaagct tttaccaatg
     2581 gaaaaccatt gtgttattat atactttcaa cacttttaaa agtaaaacag attgataaag
     2641 tgtatgtcta ttgtagtaac ccagatatta aagaatttat accagaaggt gtaacatttt
     2701 taaagagatc tagttcctta gatcaggata caacgagtat tacacaggta ttagccggtt
     2761 tcacaaaaga agtagaggct gatgtatatg taatgacaca cacaacagca ccatttatta
     2821 gcgaaaaatc tattgaaaaa ggactagatg ccgtgctgac aggagaatat gattctgcat
     2881 ttgctgcaaa aaaattgcaa gatttcttat ggaaagacgg tgaaccattt aattatgaat
     2941 tggacaatat tcctagaaca caggatttgc cgcctttata cgaagaaact agtggatttt
     3001 atatttataa tcgcaatgtg atgaccgagt tgcatagaag aattgggaaa aaaccttgca
     3061 tagttgaagt aggtgagatt gagtcagttg atattgatga agccgaggat tttatgattg
     3121 ctgatgcgat ttataatcat cttattttac aaggtagtta agacaggggg aaggttagga
     3181 tgaacaatgt acagatttta gattgcacac ttagagatgg gggctattgt aacaattggg
     3241 aatttggatt tgataataca aaaaaaatta tttctggact tatagaatca ggaattgaga
     3301 ttatagaatg tgggtttctc acaaataaaa cagtatacaa taaaaatgtt acaaaatata
     3361 cgaccttaga agaattagca cagataattc ctgaaaaaag agaaggaaaa ttatttgtag
     3421 caatgatgaa ctatggcgaa tataatatag aggatcttcc agattatgat ggttcatcta
     3481 ttgatggaat taggattgcg tttcataaaa aaaattgcaa tgaagcgttg aaattatgtc
     3541 gtgagataaa gaaaaaaggc tacttagttt ttgttcaagc tatggtatct ctcaattata
     3601 cagatgaaga gtttttagca gtaataaaaa atgtgaatca gtttaaacca tacgcttttt
     3661 atattgttga tagttttgga atgatgaaag gaaaagattt aactcgtctt ttttacatga
     3721 ttgagcataa tctagaaaaa gatatacaga taggttttca ctcacataat aacatgcagc
     3781 ttgcatattc taatgcacaa aaacttataa ccgtacaaac aaatcgaagc ctgattattg
     3841 attcttcggt gtacggaatg ggaagaggag caggtaatct taacacagaa ctatttgtag
     3901 aatatttgaa tgataatgca ggaaaaaaat atcaattaaa accattatta tatataattg
     3961 atgaaatact aaatagtttc tatcagaaga gttattgggg atattcatta ccaaactata
     4021 tttcagcagt tcataatgct catcctaatt atgcaagcta tctgtcatat aagaatactt
     4081 taacaattga agcaatgaat gaaatattcg aaatgatgga tgaagaaaaa aaggtatctt
     4141 atgataaaca atatatagaa gatttatact acaaatatat ggaaactggg aaaatacagg
     4201 aggaatatat ggaagaactg agaaaatttc tctcagacaa aaaaattcta ctgattgcac
     4261 caggaaaaag ttctgtagat gaggtagaaa gaataaaaga gtttgttacc aaagaagaag
     4321 tcgtaataat tagtgtaaat tttgaatatt ctcatataga tatagattac gtatttttaa
     4381 gtaatttaag acgttttcat gaattaccga aagaaagcag aactaaatgt attgttacat
     4441 caaatataca agcatcggat gtatatttac ggacgagtta taaaaagttg ttatctgaaa
     4501 gggaagttgt aagagacaat gcaggattaa tggcaattag atttttagcc gatatgaatg
     4561 tggatgaaat attcttagct ggatttgatg gatattcaca taatgaagat gagaattatg
     4621 gtgagcaatc catggaaatt ataacaaaat ttgcattatt agatgcaatg aacgtgcaaa
     4681 tgagagatga attgtcagaa ttagctaaaa aagtaaaggt gacattttta acggatccac
     4741 gttgggttcg aattgataag tagtagtaat aatgttttta aaattttgat aaagagatag
     4801 tgagagaata aaaatgggta aagtcttaaa taaaaaagaa gaaaaatatt taagggacga
     4861 tggggcgaga tgaaactcta attttgaact tttaagaatc atttctatat tgtgtattgt
     4921 tgcttaccat gctaatttgc gtttaaatta taatgcagac ggaattatag tacatggttc
     4981 attaactgct aaccagattt tcttgaatta tattggtatt tggggtcagc taggagttaa
     5041 ttgttttata tttatttcgg catatttttt aagtgagaaa agaaacattc actttgaaaa
     5101 tgttttggga attgtattag aatgtatttt ttatagtgta ttagtttatg gaattctttg
     5161 tgcatgtgga atagaaacat tttcattgaa cagctttgta aaagaaatat tatcatttgc
     5221 tctaggtaga tattggttta ttacatccta tttagcattg tatattctaa gtccgtattt
     5281 aaatatcatg cttaacaatc tggaaaaact tcagttgcaa aaaataagta tgttattgtt
     5341 tataggtgta tgctgttatc gtatgttatt taaaacatct ttgtttgggg attgtgctgt
     5401 tttaattgca ctatatgtca tatgttatac actgaaaaaa acgataataa tatttttgag
     5461 agacacagaa tcataggaac aattgtatgt ataacagtta taccacttct catatccata
     5521 ggaatacttt tcaaatttat accaaatgca atggttaacc agatggttca tattggttct
     5581 ccattgatgg ttatagcaag tatattttta ttttatgtat ttaaaaattt ttcattttac
     5641 agtaagtata taaataaact tgcgagaacc agtttagggg tatatatttt acatgaaaat
     5701 cattatttca ttgatggtat gaagtctaga ggcttaatat gggatgggat atttggatta
     5761 ggcaaaaggt ttttagatag ggatttttgg atttggttta taattgcagt tgtagttgtg
     5821 tgcataattt gcatggctat cgatgttgta agagaaaaat ttgaaagaca gatttttaaa
     5881 attaagagtg taaagaacct gtgtaaactt atgaatcatg ttataaatat atacccataa
     5941 ttaataaatg aggagaaaag aagtgaaagt tatatcattc ggagaattat tattgagact
     6001 tgcagcaccc ggttattcaa agttatttca aagagatagt ttggagacta cattctgcgg
     6061 aggggaagct aatgtagcgg tatccttagc aatatttgga tatcaatcag aatttataac
     6121 aaagcttccg gattcagatg ttggatatgc ggcagctaat gctttgcgtt attttggagt
     6181 aaatgttgat aatattatat ttggagaggg acgcatggga ctgtattatt tagaaaaagg
     6241 agcagctcag agaccatcta aagtaattta tgacagaaaa ttttctgcaa tttcgttagc
     6301 tgatagtgat gaatttaagt ggaaggagat cctaaacgat gcaaattggt ttcattggac
     6361 agggataaat ccagcattgt ctgagaatct ctttgagacg tccttagatg catgtcaggc
     6421 tgccaaaaaa agaggtataa caatttcttg tgatttaaat tatcgatcca agctttggac
     6481 atcagaggaa gcaaaagaaa agatggaaca gatactacca ttggttgata tttgtatttg
     6541 taatgaagaa gatgcagaaa aagtgctagg aattaaagct gagcatacaa atattaatag
     6601 tggagaatta aataaagatg gctatgtgca ggtggctagg gagattcaca agaagtttgg
     6661 atgtaggtat gtagctacaa ctctgcgtag aagttattcg gcaagtagaa atggttggag
     6721 tgcaatgtta tacgaagcag aaaaagataa agcgtatttt tccacagaat acaacataca
     6781 aattgttgat cgcgttggtg gtggagacag cttttctgct ggtataatat atgcgttatg
     6841 tgaaaatatg tccggacaaa aggctattga atttgcgact gcatctagtt gtttaaaaca
     6901 tactatagaa ggtgatttta atcgaactac aattgaggat gtatataatt taatgaatag
     6961 cggtggaaat ggtcgcgtgt tgcgttagtc aacatataaa gataaatcac agtaattttt
     7021 atggtgatta tctgtcttag tagtgattag aataacgtga aaataaattg ttgagcgttt
     7081 atactgtttt gaaaaataaa tttaacatat tgaaaaatac aaatatatac gtatattata
     7141 aagcgccgat tgtaaaatta agctagacaa ctaaaacagt catttgtgct acactcaaaa
     7201 tagttccgac caaagaatta taaggagtga gtgcaaataa cctataccta tcttacaacg
     7261 gatgaacttg taataataga gtcttatttg aaaattaacc aatctgttgc taaaactgcg
     7321 cattgcttga atcgttcaag tcaaacgatc cataaagtat atctgttctt caagcaaaga
     7381 aaatcagcct tagaata
//
LOCUS       DAYYOA010000126         7392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000126 DAYYOA010000000
VERSION     DAYYOA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.127"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..2788
                     /gene="clpB"
                     /locus_tag="ACHBKA_002430"
     CDS             179..2788
                     /gene="clpB"
                     /locus_tag="ACHBKA_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM9446086.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2939..3625)
                     /locus_tag="ACHBKA_002431"
     CDS             complement(2939..3625)
                     /locus_tag="ACHBKA_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446087.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3603..4097)
                     /locus_tag="ACHBKA_002432"
     CDS             complement(3603..4097)
                     /locus_tag="ACHBKA_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9446088.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4117..5337)
                     /locus_tag="ACHBKA_002433"
     CDS             complement(4117..5337)
                     /locus_tag="ACHBKA_002433"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9446089.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5484..7319
                     /gene="lepA"
                     /locus_tag="ACHBKA_002434"
     CDS             5484..7319
                     /gene="lepA"
                     /locus_tag="ACHBKA_002434"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM9446090.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2436 a         1406 c         1510 g         2040 t
ORIGIN      
        1 tggctctttt tttatataca tacaatcaat tttgtttttt taagatttat ataagtcgag
       61 aaaaaaagtc aaaaaactat gtgtatattt tgaccaaaat tgaccaaagc gatataatag
      121 tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa ttaggagggt atacctgtat
      181 gaatattgag aaaatgacta cgacgcttca agaagcgatt gctgaagctc aacagattgc
      241 ggtcactcga aaacatcaag atatcgacat tgcccatgta tggaaaatct ttttacaatc
      301 taatcatttt ggtcgaaatt tctatacgga tgccggattg gatgtcgaat cttttgaaca
      361 tgaaattgat cgtctactgg atgagtatcc agttgtttct ggtggaaacg tccaatatgg
      421 acaaaacctc agccagaatt tctttcgatt attgaatgaa gcagaccaaa tacgtgaaag
      481 cttcggcgac gagtttcttt caacggaagt cgttatttta gcgttgatga aattaaaaaa
      541 ttatccgtta actgtttatt tgaataaaaa cggattatct gaaaaagaat tgcgtaaaaa
      601 catcgaagag atgagaggag gagaaagagt gacctcacaa aaccaagaag aacaatacaa
      661 agcattagaa aaatacggcg ttgatttagt acagcaagtc aaaagcggaa aaatggatcc
      721 aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt attctttcac gaaaaacaaa
      781 aaataatcct gttctgatag gagaacccgg tgtagggaaa acggcaatcg ttgaagggct
      841 tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta aaagataaaa cgattttttc
      901 attagatatg ggtgcattga tcgctggagc aaaattccgt ggtgaatttg aagaaagact
      961 aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt atcctcttgt tcatcgatga
     1021 aatccacaat atcgtagggg caggaaaaac agaaggaagt atggatgccg gaaatctgtt
     1081 gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt gccacaactt tagatgagta
     1141 ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc tttcaaaagg tattagtcaa
     1201 agaaccaaca gtggaagata cgatcagtat tctgcgtgga ttaaaagaac gttttgagat
     1261 ccaccatggt gttaacatcc atgacaacgc attagtagca gcagctactt tatctgaccg
     1321 ttatattact gatcggttct taccggacaa agcaatcgat ttgatcgatg aagccagtgc
     1381 aacgattcgg gtagaaatga actctatgcc aacggaatta gatcaagtta ctagacgttt
     1441 gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa tcagatgatg caagtaaaaa
     1501 acgcttgaaa aatttgcaag aagaattagc tgaacttcgt gaagaagcaa atgcgatgaa
     1561 gatgcagtgg gaaacagaaa aagaagaagt caattctgtt tcagcaaaac gtgctgaaat
     1621 cgataaagca aaacacgaat tagaagatgc agaaaataac tatgatttag aacgcgctgc
     1681 tgtattgcgt catggtacga tacctcaatt agaaaaagaa ttgaaggaat tagaagctaa
     1741 agcaaaagac agcgagatta agatggttca agagtccgtt accgaaaatg agattgctca
     1801 agtcgttggc agattaacgg gtatccccgt aacgaaactt gtagaaggtg aacgagaaaa
     1861 actaattaaa ttgaatgaaa cacttcataa acgagtgatt ggtcaagatg aagcagttga
     1921 tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg caagacccaa atcgtcctct
     1981 aggttctttc cttttcctag gaccaactgg tgtcggaaaa accgaattag cgaaagcgtt
     2041 agctgagaac ttgtttgact cagaagatca tatggtacgg atcgatatga gtgaatatat
     2101 ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca ggctatgtag gttacgaaga
     2161 aggtggtcaa ttaacggaag ctgtcagacg aaatccttat acaatcgtct tgttggatga
     2221 aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta caagtcctag atgacggacg
     2281 cttgactgat tcaaaagggc gagtagtaga tttcaaaaat acggttctga tcatgaccag
     2341 caatatcggt tcacagttac tgctggaagg cgtgactgct gatggaacga ttccagaagc
     2401 agtggctgaa caagtcaaca cgctattacg tggaaacttc aaaccagaat tcttgaaccg
     2461 aatcgacgac acgattttat ttacaccatt gagcctagac aacgtgaaag gaatcgttga
     2521 caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa gaaatcctgc tgacaatcag
     2581 tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa ccagcatatg gtgcaagacc
     2641 attgaaacga tttatcacta aggaagtaga aacaccacta gctaaagaaa tcgttgcagg
     2701 acatgttatg cctaaatcaa aagtaacgat tactttactt gatggacaac ttcatttcaa
     2761 aacagaagag ctagaagaaa tcgtctaatc ttccattgag actataaaag aaaaaagggg
     2821 tctgacacaa ctagtgtcag acctcctttt ttcaaataaa cagtgttcca gaagtagttt
     2881 cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt ctgtcacaac ctcttttatt
     2941 aggtattttt cactttttct aatttataac tttcaaagta acgctcatct tttttacttt
     3001 gagaccatgc aaacaatccc attaaggcag cacctatcgt atttgtcagc aagtcgccca
     3061 tcgtatccat caatgctgct cgtccgacaa acagtgtgcc atcagaagca gcaaaacgtt
     3121 gaagattcat gcctaagaac tggtcgcata agaattccca gaactcccaa aacacgccgc
     3181 aaagtccagc aaacgcaaag ccgaataaga ggaacaacca aggactggtt tttgatatct
     3241 cagcatcttt catcaagtaa ccaatcaatc catagccaag agctgtcagg accattggac
     3301 tcaccgcatg taagattttg tcccaaaaag agatgatact gatcaaatgc atccctgtgc
     3361 ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa aacgattgct gctggtattt
     3421 taagtttgaa aactttcaaa atgacttgtg gcatgaaaat caatgcaatc cctaataaag
     3481 attccacgat aaatgtgatt ccttgagaag tcgaatattt ttgtgtgata aattcataaa
     3541 aattatagca gacaacgaat acaccaaaag caagtaaagc tatttgcacc tttttgattt
     3601 ctttaatcac tgaaagtccc tccatctaaa aatcttttta cttctgaaag caactcaatt
     3661 tctgaagaaa ccacttttcc gttcaatttg atcaaaccta ttgtatataa gttcaggtag
     3721 tgaaattgat tttcagctgt ttcttgtagt gcagcgattt ttttcgggtg ggttccgcct
     3781 tgctgtcgag tatcagtata taagccgatc actgggatgc ctttcgcata agccacaccg
     3841 atttccgatg ctaccccggc atcgatcgtc agcccatcta gtaaagcaat cattaaatca
     3901 ctagccaaca ctttttctgt gtcagcaaga gcaatcattc gactgtctgc ataagcagtt
     3961 ttgtcattga ttgcttcatt ttcttgagga agatagatcg aaagctcaga agagagctct
     4021 ctgatttctt taactaactg ttggttatat aacaaatcac ttttagcaaa cattggtgcg
     4081 gcaaaataaa ttttcatcta aacatcatcc tttttatcaa aaataatcgc taaagtaagc
     4141 ttccatgttt ggaatgatac gctctagtga cttcagtgtt tcttcatctg tttctaaccg
     4201 ttcgattctt gccaaataag aagcacggcg ataattcatc atcaagcaag tcaatgtttg
     4261 gatatccagt cgaacggatt ttcctatagg ttcatttaag acacggactt gtccctgttc
     4321 atcccatgtt aaagcgaaga caccattatt ccaaggagcg acaggatctt caatgatgaa
     4381 atgaaaagca tcagctgttc caacaaacgg aaaccgttga aggaattctt ttacatcaac
     4441 gatccgagcc ataaaatatg gttcgatctg ctctttgatc tgactatctt caatcaaaaa
     4501 tgctaaaggc tcatttttat aaatatctcc atgtacccag tagatcatgg aaaaatgtgc
     4561 ggaaataaag ttccataagc catttcgagc ttcttgatca agatagaaca tttcttttac
     4621 gtgaaatatt tcttcagcta cccagtagaa cagaacacct cttggttcat ggttgctatc
     4681 gtagtagaca gcggctgtgc gctcatcttc attttcgaaa cgccaatact cttcccaatg
     4741 aaaatcactg cggaataaag caccatggtt ttgttgggca aatttgtcat aaacggaaaa
     4801 tacgtctgga tgatcaactg gtaatcgctc aaccatacca cttactggga tttgtttagg
     4861 tagctgtgta tctctgattt taaaggaaag tttatcagac atgatttccc atcctttacg
     4921 ccgatagtag gggatactgt aaggatacaa ataagagatc cattgcttat tcttgcgcat
     4981 attatccaat gcaacgataa ttagatcttg catcaatcca tggttagcat attctggata
     5041 agttccaact ccagtcacac cacccatttt ataacgtgca ccgtgaatat tgacttcaca
     5101 aggatagata gcgatttgag agatcagttt attttcgtgg aaccatccga acactttcga
     5161 tacttccaaa atcggttttt tggaacggat aaaagctcgt ttattctcaa acccgctttc
     5221 ctcgatgtct gcttcagtca cttggaacac ataggaaagc aattcattga attgatcgat
     5281 atgttcttga gtgaccggtt ttaattcaag attttctcta aaatcttttt gatccatttt
     5341 ttcatctcca tttattagct tgataacagt atagcaaaat tctacaaaaa aaacttctat
     5401 agctgactaa ttaaaaactc tttaaattac tgcttttcgt tggtataatg gaaagtgctg
     5461 attatatgaa aagaggagac gctatggata taaagaaaat gaaagaacgc caagagcaga
     5521 ttcgtaattt ttcgatcatc gctcatatcg accatgggaa atctacattg gccgatcgta
     5581 ttttagaaat gacgcacact gtgacgtctc gcgaaatgca agatcagctg ttagactcga
     5641 tggatttgga acgtgaacgt ggaatcacga ttaaattaaa tgctgtagaa ctcaattaca
     5701 cagcgaaaaa tggagaaaca tatatctttc acttgattga caccccaggg cacgtcgact
     5761 tcacctatga agtctctcga agccttgctg cctgtgaggg tgccgtttta gtggtcgatg
     5821 cagcacaagg aatcgaagca caaacattgg ccaatgttta tttagcgtta gacaatgatt
     5881 tggagatcct gccagtcatc aataaaatcg acctacccgc agcagatccc gaacgtgtcc
     5941 gtcaagaaat cgaagacgta atcggaatcg atgctagtga agctgtatta gccagtgcca
     6001 aatcagggat tgggattgaa gatattctcg agcagatcgt ggaatatgtt cctgcaccaa
     6061 gtggagactt agatgcaccg ctaaaagctt tgatctttga ctctatttat gatagttacc
     6121 gcggtgttgt tctaaatgtc cggatcacgg acggtatggt caaacccgga gacaaaatca
     6181 aactgatgag taatggcaaa acatttgatg ttacagaggt cggggtcttc tcgccaaaag
     6241 cagttgctag agactttttg atggtcggtg atgtcggtta tatcacagca agtatcaaaa
     6301 ctgtccaaga tacacgagta ggggatacag taacgttagc agacaatcct gccgctgaag
     6361 ctttaccagg ttaccgaaaa atgaatccga tggtttattg tggtctttat ccaattgata
     6421 cttctcgtta caacgatttg cgggaagctt tagaaaaact gcaattgaat gatgctgctt
     6481 tacaattcga accagaaacc tctcaagcat taggttttgg gttccgctgt ggattcctcg
     6541 gacttcttca tatggatgtc gtccaagaac gcttggaacg ggaattcaat ctagaattga
     6601 tcacgactgc accatccgtt atttaccatg ttaataaaac ggatggtacg actgctacag
     6661 tagataaccc ggcagatttt cctgaacctg taacgattca ggatgtagaa gaaccattcg
     6721 tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc agtaatggaa ctttctcaac
     6781 gaaaacgggg agaattcatt acgatggatt atttagatga ttaccgagta aatgtcgtct
     6841 ataatattcc gctttctgaa attgtctttg actttttcga caaattgaaa tcaagcacga
     6901 aaggctatgc ctcattggat tatgaaatgt ctggttatca aaaaagcaag ttagtgaaga
     6961 tggacatctt gctgaatgga gaaaaagtcg atgcattaag ctttatcgtt caccgagact
     7021 ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa aaaactaatc ccacgccaac
     7081 aatttgaggt gccaatccaa gcagctattg gacaaaaaat cgttgcccgt tcagacatca
     7141 aagccttgcg taaaaacgtc ttggctaaat gttatggtgg agatgtttct cgtaaacgta
     7201 aactcttaga gaaacaaaaa gaagggaaga agcggatgaa acaaatcggc tccgtcgaag
     7261 ttccgcaaga agccttcatg gccgttctga aaatggacga ggacgaaccg aagaaatagc
     7321 aagggtttgt ttgaaaacag tgtaaaaaat aaaccatgtt tacatggatt tgccaccggt
     7381 ttgccaccgg tg
//
LOCUS       DAYYOA010000127         7066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000127 DAYYOA010000000
VERSION     DAYYOA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.128"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1778..2443)
                     /locus_tag="ACHBKA_002435"
     CDS             complement(1778..2443)
                     /locus_tag="ACHBKA_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9446091.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2569..4605
                     /locus_tag="ACHBKA_002436"
     CDS             2569..4605
                     /locus_tag="ACHBKA_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM9446092.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="ACHBKA_002437"
     CDS             4609..5247
                     /locus_tag="ACHBKA_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9446093.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="ACHBKA_002438"
     CDS             5275..5703
                     /locus_tag="ACHBKA_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446094.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6160..>7066
                     /locus_tag="ACHBKA_002439"
     CDS             6160..>7066
                     /locus_tag="ACHBKA_002439"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9446095.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASS"
BASE COUNT         2421 a         1064 c         1306 g         2275 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
     5761 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
     5821 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
     5881 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
     5941 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
     6001 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
     6061 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
     6121 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
     6181 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
     6241 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
     6301 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
     6361 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
     6421 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
     6481 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
     6541 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
     6601 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
     6661 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
     6721 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     6781 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     6841 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
     6901 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     6961 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     7021 aatgttttga gtacattcac ttcagattct agtacagcaa gtagta
//
LOCUS       DAYYOA010000128         6789 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000128 DAYYOA010000000
VERSION     DAYYOA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.129"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..573)
                     /locus_tag="ACHBKA_002440"
     CDS             complement(115..573)
                     /locus_tag="ACHBKA_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9446096.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(586..1917)
                     /locus_tag="ACHBKA_002441"
     CDS             complement(586..1917)
                     /locus_tag="ACHBKA_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM9446097.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1928..3370)
                     /locus_tag="ACHBKA_002442"
     CDS             complement(1928..3370)
                     /locus_tag="ACHBKA_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9446098.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3357..4121)
                     /locus_tag="ACHBKA_002443"
     CDS             complement(3357..4121)
                     /locus_tag="ACHBKA_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM9446099.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4225..5100)
                     /locus_tag="ACHBKA_002444"
     CDS             complement(4225..5100)
                     /locus_tag="ACHBKA_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9446100.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5355..5439)
                     /locus_tag="ACHBKA_002445"
     tRNA            complement(5355..5439)
                     /locus_tag="ACHBKA_002445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5403..5405),aa:Leu,seq:caa)
     gene            complement(5457..5528)
                     /locus_tag="ACHBKA_002446"
     tRNA            complement(5457..5528)
                     /locus_tag="ACHBKA_002446"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5494..5496),aa:Gln,seq:ttg)
     gene            complement(5538..5610)
                     /locus_tag="ACHBKA_002447"
     tRNA            complement(5538..5610)
                     /locus_tag="ACHBKA_002447"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5575..5577),aa:His,seq:gtg)
     gene            complement(5672..5756)
                     /locus_tag="ACHBKA_002448"
     tRNA            complement(5672..5756)
                     /locus_tag="ACHBKA_002448"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5720..5722),aa:Leu,seq:caa)
     gene            complement(5777..5847)
                     /locus_tag="ACHBKA_002449"
     tRNA            complement(5777..5847)
                     /locus_tag="ACHBKA_002449"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5813..5815),aa:Cys,seq:gca)
     gene            complement(5893..5964)
                     /locus_tag="ACHBKA_002450"
     tRNA            complement(5893..5964)
                     /locus_tag="ACHBKA_002450"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5930..5932),aa:Gln,seq:ttg)
     gene            complement(5976..6048)
                     /locus_tag="ACHBKA_002451"
     tRNA            complement(5976..6048)
                     /locus_tag="ACHBKA_002451"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6013..6015),aa:His,seq:gtg)
     gene            complement(6051..6124)
                     /locus_tag="ACHBKA_002452"
     tRNA            complement(6051..6124)
                     /locus_tag="ACHBKA_002452"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6090..6092),aa:Trp,seq:cca)
     gene            complement(6131..6211)
                     /locus_tag="ACHBKA_002453"
     tRNA            complement(6131..6211)
                     /locus_tag="ACHBKA_002453"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6175..6177),aa:Tyr,seq:gta)
     gene            complement(6213..6288)
                     /locus_tag="ACHBKA_002454"
     tRNA            complement(6213..6288)
                     /locus_tag="ACHBKA_002454"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6253..6255),aa:Phe,seq:gaa)
     gene            complement(6324..6396)
                     /locus_tag="ACHBKA_002455"
     tRNA            complement(6324..6396)
                     /locus_tag="ACHBKA_002455"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6361..6363),aa:Asp,seq:gtc)
     gene            complement(6406..6478)
                     /locus_tag="ACHBKA_002456"
     tRNA            complement(6406..6478)
                     /locus_tag="ACHBKA_002456"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6443..6445),aa:Val,seq:tac)
     gene            complement(6494..6565)
                     /locus_tag="ACHBKA_002457"
     tRNA            complement(6494..6565)
                     /locus_tag="ACHBKA_002457"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6530..6532),aa:Glu,seq:ttc)
     gene            complement(6580..6669)
                     /locus_tag="ACHBKA_002458"
     tRNA            complement(6580..6669)
                     /locus_tag="ACHBKA_002458"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6633..6635),aa:Ser,seq:gga)
     gene            complement(6709..6782)
                     /locus_tag="ACHBKA_002459"
     tRNA            complement(6709..6782)
                     /locus_tag="ACHBKA_002459"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6746..6748),aa:Asn,seq:gtt)
BASE COUNT         2051 a         1499 c         1105 g         2134 t
ORIGIN      
        1 cgataattaa caaaaatcaa aatgattaaa tctagtgatt gaaacatatc atttcaaaaa
       61 aattttgtaa attcacccat aaatatcacc ctatttaact tttttcatcg tacactaaaa
      121 ttcaaaacaa ctatctttaa tctttacttc ttggttttgt tttaataaat ttccttcaaa
      181 aatgcctgga gaaactacaa tgatcaccgt atctttttgt gcttgctcca acttcttcaa
      241 atctataata gctaacagtg tcccttcttt tacctcctgg ttactttcaa cccgtatctg
      301 aaacggactt ccttcgagtt caactgtatc taaacccata tggatcagta tttttctgcc
      361 agattcactt tcgatcgtta tggcatgtcg ggtaggaaat atattatcga ttattccttt
      421 aactggcgca tggatatatc catcatgatt cgttatagcg tatccaactc ctatcatttt
      481 tgaagaaaag acctcgtctt ttactgctgt taatggaata atttgaccac tagctacagc
      541 ttttagaatg tttcttgttt taaagaaatg cactttttat cacctctact ttaatgctgg
      601 ccaatatttt ttgtttactt tgatcatttc atctagtatt ttttttgcga ctctggcatt
      661 tggtacggtt tgattcaatg taaatgcttg taaagctttt tgataagaac cttcaaagta
      721 agcatctact agtaacttct ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc
      781 actgattggt tttcttagtg taattggctc cacaccagtt gcaccaacat atgccggtat
      841 ttcaacaact gcatctgacc gtagattcgg aatcgcccct tgattaggaa cgattagcat
      901 aaagcgacga cgatcatcat tcaacaagga aatagctata tcgacgatat attgtccatg
      961 ctccccaaaa ttaaagttca atacttcttc cacaataccg cggcgtattt tatcagccat
     1021 ttctttggta tttttctctc gtccatccat gaccatatta gcacgtgtat actctttgtc
     1081 agtatgctca acgaccatat caggatataa gtaatactct aagtaattat tcgggatatt
     1141 acttggaaaa tttttcgtga tgactgacat catttgaaag gttttttgcc aagttttatc
     1201 tcctatatta aaatcggcga cttgcatttc ttttgttatt agtttttcaa taatctctgg
     1261 cataatttct cgttgcaatt ctttatcata aatcgatgta taccagccaa agtggtttaa
     1321 accataatac gtaacgatcc aattcttacg atcataaccg taattcactg caatcgtttc
     1381 ttcaattgaa atagtcatat cacaggcatt aatcattttt acattcggat atttacgacg
     1441 aacagcttca gaaacgatcg actctggatt tgtataattc aaaatccatg ctttgggggc
     1501 atagtcttgt atatgatcga caatttccaa taaacctttg attgagcgca tcccataagc
     1561 aaatcctccg agtccacacg tttcttgtcc tactaatcca tgtttaagtg gaatcttttc
     1621 gtccttttct cgcatctcca atcctccaac acgaatttgt gagaaaacaa aatcgcatcc
     1681 tgtaaaagca agctttggat catccgttgc acgaatgcga atatttgaaa aaccttttct
     1741 tttcagcata aatttaacaa tcaagtacat atccatattt ctttcttcat caatatcata
     1801 taaacgtatt tcagataatg gcaaccgttt ttcattgttc aaaatggctt gaacgattcc
     1861 tggtgtatac gtactcccgc ctccagctat agtgataatc tgttttttca tcatcatctt
     1921 ttcctttcta ttcttctaat aatttattga attctgccat aacataagtc acttctaagc
     1981 cgatgataat ttgataattt ttttcagttg gtttaatgat cccgcttatc cctaattgct
     2041 tgattcgctg ttcctcgacc atggtgggat ccactacctc tacccgtagg cgtgtattac
     2101 aactatagac atccaggata ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca
     2161 tatattcata atttttactt gctaacccta actctgtttc ttctaaggtt acttcattgc
     2221 caaactctgc cttagcttca cgtcctaatg tctgaatgtt tctcttattg atcaaataat
     2281 aaaaagtaac gtaatacaag gcaaaaaaga aaattccgat tggaagaatc aaaatagcat
     2341 tggtagcgat tcgaaaattg atgagataat caacaataca tgctccgaat gtaaaaccaa
     2401 ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca
     2461 cgtacaacat aggagaaaga aacatgaaac taaattcaac aggctcagtg atatttgcaa
     2521 taaatgaagt tcctgcaccg cttcccatga ttcctttgac catctcttta ttttctttat
     2581 aagctgcttt atagatagct agacatatag ctggtatacc aaacatcatt actacaaaaa
     2641 aaactgataa aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc atctctcctc
     2701 tgaaaacttc cttgctagga gatgtatagc ttcctaaatc gtaataaata tacgtattca
     2761 acacatggtg cagtccaaag gggatcagca aacgattcaa aaacataaat atccccacac
     2821 ctaatgctcc catatcaacg agcagttttg caaaattatc aatcccaatt tggatgagcg
     2881 gccagataaa gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc atagtcaatg
     2941 ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct ttaaagcgat
     3001 tatacgtcca tgccgctact aaaccggaaa taattccacc aaaaatcccc atattcactt
     3061 ctgggtccat aatttctaag ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg
     3121 caggtattcc tttgtcctta tttttgacaa aaccgattgc aactcctata gcaaataaga
     3181 tatccaagtt tccaaaaacg gcatttccag ctgcaccaat tactgggatg ttcagcatat
     3241 cgtcagctga taaacgcatg atcaatcccg ctaatggcat cgctgcaact ggaagtagca
     3301 tcgctcttcc aagcttctgc acatttgatt taacattcat ttttattcct cctctattaa
     3361 atatcctcat atgttactaa ctcaatcaaa ggatcatttt tcactttttg agcaaatgct
     3421 gaatgaatta aaaattcgac ttggtgggta cgaggataca caaaacttga accatttaac
     3481 aattcctgat ccaaatacgc tgcgtgacac atgacttcga tcgttttatc ttctctcaat
     3541 atcatgagta aacttgccaa atattcttcc acactcgtat tgatttgtcg ttttttttca
     3601 tcagtttctc ctacgacatc gaaacaaggc tcaaaataat cgacatgacg aacttcatct
     3661 tttcgttcaa agttgctccg aaccggcaaa tcatattttt ttgctaatgt aacaactacc
     3721 tcctgatttt catccaaagt atgagtatga tgatgactgt ctaaatgagt tggtatgatc
     3781 cccgcatgat aaattttttg aatctgagca ttccattcct gatacagttg atctttgtca
     3841 atgttaaatg gatgctgata ataagataag gatctgaact ctccgctttc gataagagta
     3901 tcgacagttt tgaggagtgg tttgccacac gtcaatgtta aatggactcc tacacctagc
     3961 ctaggcattt cctttcttag tttcacagca tgctcgaaac ccggcatatt tgccatcaat
     4021 gtagttgatg tcaaaattcc tcgctgataa gcatccataa tcccataatt cacaccatgg
     4081 ctatagccaa aatcatccga attaaaaatc acttttccca tttcattctc cttctttttt
     4141 ctactaaaat aggcgcaacc aaataatcat agtccattta ctatgtgtca tttggttgcg
     4201 cctatagata gttacgccct tattttaata gattcttgct agatgaattg ccaaatagat
     4261 gatttcattt tcttgaatgg caagttgaaa ttgctgttcg atgatatctt taatctttag
     4321 tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa tattttctac
     4381 tggcgtttct tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg
     4441 atgataagtc agtgattctt tatcataaac gtttgaatag gatttttcta gatgggacaa
     4501 aatcgtggtc gtcatttcta ctatttttaa gctctttaaa ctattaggct ctcctagctc
     4561 agcattaatg atatgcatag tgatgaatcc tacttcattt gaccctaact cggtttgata
     4621 tcgatgattg attcgctcga ccacttctac tgctatatca tattcatttg gataaaggaa
     4681 tctcatttct ggcaacataa ttgctgtgac atctctatct tctctttttc gttgaatagc
     4741 aaaataaata tgatctgtca atgtaatata gatatttgaa tctaatggag aggagatcct
     4801 agctgttatc agttcaatca cttcttccgc aaaatccata aattcgattg gaatctgcga
     4861 gactatctca ctgaatcgtt tgatcttttc tttagaatct aatacaaata atttgtcagc
     4921 atcctcaaca tcaaatggct tactcacttt agcagaaaag ccaatccctt tccccatcgc
     4981 aattttttct attccatcgt catcgatcaa tacagcatta tgattcagct ttttcatcac
     5041 atacaccgtt aattcacctc ctctatatga taaaacaaaa caacttgcag actccttcat
     5101 acatattttt gtctccaaac aatttttaaa gacaaaaaac gacctaagaa agaacccttt
     5161 cttaggtcgt gcctaattga atagtagcac cctacataat ttacaaaaat agtttaacac
     5221 ataataaaag cgttatcaat atctttttta ctcgttttat gataatactt gtatcgaatt
     5281 gatttggagg taattacata aaaacaaaaa aaatatagca actctctata agaaagttgc
     5341 tatattgata attataccgg cggccggggt cgaaccggca cgtcctcaac ggacactgga
     5401 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca aatttaaaaa taataactgg
     5461 ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct
     5521 ataccccaag aataaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
     5581 ccggtgcgtt aaccacttcg ccacattcgc catcataaaa gatgttattt aatttctcaa
     5641 taaaaaacta agatgaagac atcttagtca ataccggcgg ccggggtcga accggcacgc
     5701 cctcaacggg cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaagt
     5761 tgatttatta gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc
     5821 tctgccttac cacttggcta taccgccatt atactattat caatctataa aataaagaat
     5881 aaattataga aactggggta gctggattcg aaccaacgca tgagggagtc aaagtccctt
     5941 gccttaccgc ttggctatac cccaagaata aaaagggcga atgatgggaa tcgaacccac
     6001 gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc
     6061 agcaggaatt gaacccacac taacggtttt ggagaccgct gttctacctt taaactatgc
     6121 ccctataaaa tggaggagag tggattcgaa ccactgaacc ctaaggaacg gatttacagt
     6181 ccgtcgcgtt tagccacttc gctactcctc catggtggcg cgggacagaa tcgaactgcc
     6241 gacacacgga gcttcaatcc gttgctctac caactgagct accgcgccat attttaaaat
     6301 tattaattga ataacaagaa cgacggttcc gacgggaatc gaacccgcga tctcctgcgt
     6361 gacaggcagg catgttaacc cctacaccac ggaaccagtt cattatggag gttaacggga
     6421 tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc cagctgagct aaacctccaa
     6481 cattcaatta aaatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt
     6541 gtcttaaccg cttgaccaac gggccaggta aatttgctac ggagagtaag ggattcgaac
     6601 ccttgagaca gtttggcacc atctacatga tttccaatca tgctccttcg gcctctcgga
     6661 caactctcca gagtgaaaga agctcaatac tctttctcaa aatgagaact ccggcagtag
     6721 gactcgaacc tacgacatca tgattaacag tcatgcgcta ctaccaactg agctatgccg
     6781 gaataacat
//
LOCUS       DAYYOA010000129         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000129 DAYYOA010000000
VERSION     DAYYOA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.130"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHBKA_002460"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHBKA_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHBKA_002461"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHBKA_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM9446101.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHBKA_002462"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHBKA_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHBKA_002463"
     CDS             complement(2143..2373)
                     /locus_tag="ACHBKA_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM9446102.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHBKA_002464"
     CDS             complement(2603..3421)
                     /locus_tag="ACHBKA_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9446103.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHBKA_002465"
     CDS             3582..4271
                     /locus_tag="ACHBKA_002465"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9446104.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHBKA_002466"
     CDS             4285..5787
                     /locus_tag="ACHBKA_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9446105.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHBKA_002467"
     CDS             5800..6276
                     /locus_tag="ACHBKA_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9446106.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHBKA_002468"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHBKA_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYOA010000130         6570 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000130 DAYYOA010000000
VERSION     DAYYOA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.131"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHBKA_002469"
     CDS             127..579
                     /locus_tag="ACHBKA_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446107.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHBKA_002470"
     CDS             593..1282
                     /locus_tag="ACHBKA_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446108.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHBKA_002471"
     CDS             1407..3758
                     /locus_tag="ACHBKA_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM9446109.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHBKA_002472"
     CDS             3838..4233
                     /locus_tag="ACHBKA_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446110.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACHBKA_002473"
     CDS             4558..5175
                     /locus_tag="ACHBKA_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9446111.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACHBKA_002474"
     CDS             5216..5515
                     /locus_tag="ACHBKA_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM9446112.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACHBKA_002475"
     CDS             5549..5716
                     /locus_tag="ACHBKA_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9446113.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACHBKA_002476"
     CDS             complement(5761..6366)
                     /locus_tag="ACHBKA_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9446114.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAYYOA010000131         6453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000131 DAYYOA010000000
VERSION     DAYYOA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.133"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            221..655
                     /locus_tag="ACHBKA_002477"
     CDS             221..655
                     /locus_tag="ACHBKA_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9446115.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="ACHBKA_002478"
     CDS             678..998
                     /locus_tag="ACHBKA_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446116.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="ACHBKA_002479"
     CDS             1139..1330
                     /locus_tag="ACHBKA_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446117.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="ACHBKA_002480"
     CDS             complement(1401..1658)
                     /locus_tag="ACHBKA_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM9446118.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="ACHBKA_002481"
     CDS             1816..2079
                     /locus_tag="ACHBKA_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446119.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="ACHBKA_002482"
     CDS             2103..2291
                     /locus_tag="ACHBKA_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446120.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2374..2559)
                     /locus_tag="ACHBKA_002483"
     CDS             complement(2374..2559)
                     /locus_tag="ACHBKA_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446121.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            2774..3145
                     /locus_tag="ACHBKA_002484"
     CDS             2774..3145
                     /locus_tag="ACHBKA_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9446122.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3295..3367)
                     /locus_tag="ACHBKA_002485"
     tRNA            complement(3295..3367)
                     /locus_tag="ACHBKA_002485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Gln,seq:ctg)
     gene            complement(3413..3841)
                     /locus_tag="ACHBKA_002486"
     CDS             complement(3413..3841)
                     /locus_tag="ACHBKA_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446123.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(3901..4659)
                     /locus_tag="ACHBKA_002487"
     CDS             complement(3901..4659)
                     /locus_tag="ACHBKA_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9446124.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4954..5151
                     /locus_tag="ACHBKA_002488"
     CDS             4954..5151
                     /locus_tag="ACHBKA_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446125.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5257..5739
                     /locus_tag="ACHBKA_002489"
     CDS             5257..5739
                     /locus_tag="ACHBKA_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446126.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(5797..6393)
                     /locus_tag="ACHBKA_002490"
     CDS             complement(5797..6393)
                     /locus_tag="ACHBKA_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9446127.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2178 a          995 c         1274 g         2006 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
     2341 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2401 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2461 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2521 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2581 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     2641 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     2701 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     2761 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     2821 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     2881 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     2941 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3001 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3061 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3121 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3181 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3241 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3301 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3361 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3421 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3481 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3541 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3601 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     3661 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     3721 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     3781 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     3841 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     3901 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     3961 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4021 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4081 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4141 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4201 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4261 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4321 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4381 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4441 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4501 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4561 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     4621 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     4681 gtttatcttt cctattataa atgtattttt attggaaatg gaagtattat attttaatat
     4741 gtaggaaata attagttttt aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa
     4801 tacaacagct gttttaattg tgaaaacgaa aacagtacgc tataatgcct gtaaggttga
     4861 tttttaactg taaataaaaa tcaatgagtt aaatctccaa agaaatagca gtggtaatgt
     4921 ggagaaaagt tccagcagtt taggaggatt ttgatgacgg aagaaatgat taatttagga
     4981 gaacaatatg catgcaaacc aattggtttt acaaaaacgg tcattggtga agttgtatca
     5041 aaaatgacaa actgcgccgt ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta
     5101 ttagatgaaa aagcaagcat ggttgttgca aaatatgata catttgaata aagaaaacaa
     5161 ttggacgtgc ggcgtgctgc aagtccaatt tctttgagaa ggtaaatcag tattcttttc
     5221 ctattttctg gagaagaaaa agcgaggcga gttgtcatgg taacattctt tacggttcta
     5281 ttgtttttat tcgttgttgt gatgttttta tttctttggc tggttcgaaa agaaattatt
     5341 tatcggactg tgcgaaatcg atgggtctac ttggtgatac ccttcttagt gtcactagtc
     5401 atttggtata cactcatcag tcatcctaca gcagatgaat tggcaaaagg gatcttgtca
     5461 gcaatgattt ttattagttt tttgttggac agtagaggaa tcacagaaga aggacttgtt
     5521 ttgaatagct ttgacaaaaa aggtgttcct ttttctgaga tcaataaaat tgtattatat
     5581 cagccaaaag gttctaaaat cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg
     5641 atgctgaaat tttctgcatc attagaagaa ttagttccat ttttgtccca acgcttaaac
     5701 gatgaggcag agattgatat tatgattgat ccagaataaa aacagaggtt gcgacaagat
     5761 gtttgtcaca acctctgttt tttatttcaa ataaaattat ccaaatacgc ggaagcgatc
     5821 ggcatcgttc atgtattctt tttccccagc cggtgtttct ggtgaaccga atactaattg
     5881 cgaacgtagt ttccagtttg aagggatatt ccattctttt gcaactgctt cgtcaataac
     5941 tgggttgtaa tgttgcaagt ttgcgcccaa tcctttatca actaatgcta cccaagtatt
     6001 agctgttgcg attccgtttg attgttctga ccaatctggg aagttgtctg catataattc
     6061 aaattgttct tgtagacctt ttaccacgtc agtatctttg aagaataata ctgtaccata
     6121 accatttttg aaaccagcta atttgttttg tgtatttggg aaagcttctg ctggagtcaa
     6181 tgggcgaagt gcttcttcag tgatttccca aagtttttca tgtgcatctc cgaaaaggat
     6241 aactgcgcgt gttgattgag cattgaaagc agttgggctt tctttaattg cttctttgat
     6301 caaagttgtt aattcttcat tagaaagtga aacattacgt cctagatgat agattgaacg
     6361 gcggtttttc aaagtgtcag taaaagttgt cataataaaa tttctccttt gatttgcgtt
     6421 ttgcattttt ttaattgatt acgaaatgaa gta
//
LOCUS       DAYYOA010000132         6441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000132 DAYYOA010000000
VERSION     DAYYOA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.134"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(177..725)
                     /gene="aac(6')"
                     /locus_tag="ACHBKA_002491"
     CDS             complement(177..725)
                     /gene="aac(6')"
                     /locus_tag="ACHBKA_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9446128.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1278..2381
                     /locus_tag="ACHBKA_002492"
     CDS             1278..2381
                     /locus_tag="ACHBKA_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9446129.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2378..2584)
                     /locus_tag="ACHBKA_002493"
     CDS             complement(2378..2584)
                     /locus_tag="ACHBKA_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446130.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(2574..3641)
                     /gene="rlmN"
                     /locus_tag="ACHBKA_002494"
     CDS             complement(2574..3641)
                     /gene="rlmN"
                     /locus_tag="ACHBKA_002494"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9446131.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3871..6318)
                     /locus_tag="ACHBKA_002495"
     CDS             complement(3871..6318)
                     /locus_tag="ACHBKA_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9446132.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         1805 a         1381 c         1101 g         2154 t
ORIGIN      
        1 acaaaatttt tcttactact cgaaactaag cgactttttc acaacctctg gtacacggtg
       61 ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa gaagtaattt
      121 tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc tttttattat
      181 tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt gtcccagcca
      241 tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa ttcatacgga
      301 tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata caggtccgtt
      361 tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt gattcctcct
      421 ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg gttctttcgt
      481 cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta ttgagggatt
      541 gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat tcgttctgga
      601 ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg ccaagtcagt
      661 ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa ttcactgatt
      721 atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa aaacgaaatg
      781 ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt atttatctta
      841 cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag atcaggaaag
      901 ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc ttttgcttta
      961 aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat cttaattttt
     1021 agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta agaggaacaa
     1081 aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga tacttattga
     1141 tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg ttcacctcta
     1201 ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg tttcaacgaa
     1261 gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc attggaacag
     1321 atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta gtgctgattg
     1381 gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg acacaggaaa
     1441 tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat attgaacagt
     1501 tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt ggtggccgtg
     1561 cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc acctttccca
     1621 ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac gaagaaggcg
     1681 taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt gatacagcaa
     1741 ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct ttatccaaag
     1801 aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat agattaggaa
     1861 tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa gcattgaaag
     1921 atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat atcattgtta
     1981 ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg gcacactcat
     2041 tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga gcatctgtca
     2101 gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt gataaaatct
     2161 atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat ctcaatgtca
     2221 cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt aaacatgtgc
     2281 cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa acttatcatg
     2341 ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct aaaaccccca
     2401 tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt tttcttgtca
     2461 aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg aacagttcct
     2521 taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata cgattatttg
     2581 gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc aatatctgta
     2641 ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc ataaaatctc
     2701 aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg attatatgga
     2761 atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa ttgttgcgca
     2821 tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat tcgacggttt
     2881 gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa acttcggttg
     2941 atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat agccagattg
     3001 acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact agtggaaact
     3061 gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg caaaaagtcc
     3121 attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac atgtgaaacg
     3181 cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg tgcaacaatt
     3241 tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa cgtacagccg
     3301 atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg tctcatcagt
     3361 acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt accatccgaa
     3421 gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga taatttttcg
     3481 atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt ttgatacaac
     3541 cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa ccaattgatt
     3601 aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc cctctttttc
     3661 tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact tcacaaataa
     3721 atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac cgtattagaa
     3781 aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt ttattggtta
     3841 ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg gcaacgtttc
     3901 tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg acgggccaac
     3961 aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac cgatccgaaa
     4021 tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg cttttgcttc
     4081 ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt catctgtcgg
     4141 catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa cgacccgttc
     4201 tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct cacccattgg
     4261 caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat caatgatcgt
     4321 ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat ttgctttgat
     4381 gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat gaatccccgc
     4441 tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc tggctaccat
     4501 catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg gatgtttcgt
     4561 tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat ttcttactcc
     4621 ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt gtaaggcttg
     4681 cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc cgttgtacat
     4741 attcaattca accattttcg gatcgatcat catcagtttt acttcatggg gcttagcacg
     4801 cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac cagtcgaacc
     4861 agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc cagaaacatc
     4921 acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt cgatgatttc
     4981 tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca gtgatttacc
     5041 tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga tatcatctgt
     5101 taagttcaca attttactta cttttacccc tacagccggc tggacttcga attttgtgac
     5161 ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt ggaaagtttg
     5221 ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat ccacagttgg
     5281 tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct gttctgcttc
     5341 aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa aaccttctgc
     5401 tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag gatggatatt
     5461 ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat tttctggttc
     5521 ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct tctcatattc
     5581 tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt tcgcttctgc
     5641 gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg caggatcacc
     5701 ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca gctgatagcc
     5761 ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg ctaaataact
     5821 tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa tcatcccacc
     5881 accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat cccatgtcgt
     5941 ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat gtaacaataa
     6001 taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac caattgaaaa
     6061 ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac agaggatttg
     6121 gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta aaaaacctaa
     6181 ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa tcgtcagcat
     6241 ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt ttttagtagg
     6301 ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat acaaaaaaaa
     6361 gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt tcagcttatt
     6421 ttacagtttt tcttaatttc t
//
LOCUS       DAYYOA010000133         6372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000133 DAYYOA010000000
VERSION     DAYYOA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.135"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2563
                     /locus_tag="ACHBKA_002496"
     CDS             <1..2563
                     /locus_tag="ACHBKA_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM9446133.1"
                     /translation="IFSTADSQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVR
                     DNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNG
                     YHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAV
                     KGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPF
                     IQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIR
                     FHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQ
                     EEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLI
                     IKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNS
                     CYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQ
                     GKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQ
                     NNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRY
                     IEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWA
                     VLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSE
                     QTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRR
                     EFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2745..2984
                     /locus_tag="ACHBKA_002497"
     CDS             2745..2984
                     /locus_tag="ACHBKA_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446134.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3000..4373
                     /locus_tag="ACHBKA_002498"
     CDS             3000..4373
                     /locus_tag="ACHBKA_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9446135.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4377..6011
                     /locus_tag="ACHBKA_002499"
     CDS             4377..6011
                     /locus_tag="ACHBKA_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9446136.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6174..>6372
                     /locus_tag="ACHBKA_002500"
     CDS             6174..>6372
                     /locus_tag="ACHBKA_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446137.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2231 a         1041 c         1287 g         1813 t
ORIGIN      
        1 tattttttct actgcagatt ctcaagcaat tattaactat ttagaaaaac agaaattgtc
       61 tgaaggtaca gtaaacataa cttataatcc tattaaacaa gaggtgatta cacaaaaaaa
      121 gcattctgtg agagacaatg atattgaaaa aattcggttt gtatttattg atattgaccc
      181 taaaaggaaa gagggaagtg caacagatag cgaaaagaaa aaggcagaaa atgttatgga
      241 acaagtagaa cgttatttga aagaaaagag aatagaatca tttgtaaaag ttgatagtgg
      301 gaatggctac catatttttc ttcccataaa tgaacaacca aataatcacg agacgattct
      361 tactattcag aattttcttc aattacttca ccgaagattt ggagtagaag atgaagttga
      421 tattgatact tctgtttata atccttcaag attatgcaaa ttagttggta caccagcggt
      481 caaaggaaag tccacaaaag agcgtccgca tcgtttatct agtttccttt ctattcccga
      541 taacatagat agaagtaatt gttttaaaat tcttgaacag atagttgaag aaaattccat
      601 gatgtcaacc tctagagtaa aaagccatcc atcttttaga gaagtgttac cttttattca
      661 agcagactat aaacaatggt tagctcacta tcctgaatta acttatacca taaaagaggg
      721 cgatcataaa ggagttacct tacttatatt tgacgaatgt ccattaagaa aacacacgaa
      781 taattctaat ggttcttgtt tgtctgttac ggctgataat aagattcgat ttcattgtct
      841 gcatgcttcg gataaaaata aagatattca agactttgta aaaaaatatc cgttgccaaa
      901 acctgtaaat ccttctgtag aatcattaga atccaatcaa gaatattatt ttgaccagtt
      961 taaactgaat caagaaggag tatttttggt gaaagaaggt caagaagaaa aagttgcttg
     1021 tccgatgttt attaagcaaa tacgcaaaaa tatcgaacaa aaagtcatta cacttgttct
     1081 tgcctattat gtcgatcatt gttgggatga aatagaaata gaaggtggat gtttacaaac
     1141 taataatttt aaacaattag caaaacatgg cttaatcatt aaagctagaa gagaagctga
     1201 agtcgttgac tttttacaga ttcaacggga gaatgctcca atcttttttg aacatgaatc
     1261 tcttggctgg aaaaaagaaa atgaagcaat gatttttcag ttacaagatt cttttggacg
     1321 tgaaattcct tctgtattaa ctaaaaactc atgctaccaa ttaacaacta atggtacaga
     1381 agcggaattt gacgcattgg ttcataagta tgttttagga acgaacttag aacttgcttg
     1441 gtcgattggt attgcttcta ttgtgttagg ctatttaaac gttacgaagg ctaatacttt
     1501 tttgagttta ctaattaacc tacagggcaa gagcacaacg ggtaaaacca ctacgttaca
     1561 ctttattgcc tctttatttg gaaatccaac tacggttctt agaaatatga atgctaccaa
     1621 taaagccatt ataaagctag catcaaacaa tcagggaggt gttccactaa tactagatga
     1681 gttaggagca gcaactcaaa ataactttag ctcgttgatc tatcaattag cttcaggaga
     1741 agaaaggcta agattagatt ctaattgtca gcttatgcca caagagactt tttctgtttt
     1801 gacccttacc tcttctgaag agcgtttgga aaattatttc aatgagaaat taactggttt
     1861 gaaagttcga tatatcgaat tttctgatat caagtttaca aaggatgcga atcacgcaga
     1921 gaaagttaaa agtctttctt ctaatacttt tggttggggt ataaagttat ttcttttgaa
     1981 actctttgaa caagggatta caaaaattga agcaactttt gtcgaagctc aaaaaaaact
     2041 atgggctgtt ttacctgaac attctttgaa gcaacgcatt ggtaataatc tagctttgat
     2101 ttatagtgga gccctcctaa ttcaagatat actgggctat cctttacgat tagagcatat
     2161 acagacggtg cttattaatt cgtacgaaca gttaattgta gatactagtt taggaagtga
     2221 gcaaacggta tttgaccgag tgaaaacatt gttggtgatg aatagccata aatttttaac
     2281 aaacagtgac aacaaacata cgcccagtac gatttgggga aaagtatcaa ttcaaaaaaa
     2341 tggggatatt aaggtaaata tttttcctat cccgtttgag gacttgttaa ggcgagaatt
     2401 tcaagtaaag gatctaacct atctttttag gcaattgctt gattcgggta atatgtgtgc
     2461 agaaaaaggg agaagaacga aacgtattca catgaatcgt caagttcttc caacatacga
     2521 gattctatta ccttcttcac tcaaatcgta ttttcgattg taatttagaa taaaccaaag
     2581 tatgaaagtg aggcaccatg ttatggaaat atttcttatt gatttagcaa aacaactgat
     2641 tacttatctg tatgatagcg agcaaaccca aacggaagta aatgaagctt gatctatgct
     2701 tcatttactt cattcaatca aacatgaaag aaaggaaaat tctaatgact aaaaataaaa
     2761 tgacactaaa agcagaggtt cttctgtata ttcaagaaca tttttcaaat caagcctttt
     2821 ttacaaaacc aatttacttg gatttcgaaa taagaggggt atctgctgga tctatcgggg
     2881 gtactcttca agcgttgaag aacgaaggat atttagaaaa tcactttgtg caacgttcgt
     2941 tcaatagaag agtcgtgaaa aaatggtatt tagttcattc atagagaggg gaatcaagca
     3001 tgcgtgtggc ttgttatgat cgaacgtctg tttatgaaga agcgaaaatt agtaatgacg
     3061 ctcaatatga tgcagtgaaa gagtatgttt tagagaaata tgaacaaatt ccccaacgat
     3121 tttcagacaa aggagaatca ggggaaaaag cggagcgtcc agcttttcga cagcttagag
     3181 cttgggtttg tgcgtcagaa gaagaggggg tgatctatgt ttggcgttat gatcgattgg
     3241 cacgtcaagc caaaatagcc ttagaattta ttgagttgtg ccaacaacat caggttcaag
     3301 tgtattcgat ttctgaacct cttcctgctt ctttctcaca cgcgtcaacg aataaactat
     3361 ttgtgcaatt attgttcact tttgcagaac aacaacgaaa cgtcattatt gagaatgttc
     3421 gtgccggact ttcctataaa aagcaacaac ggcaatattt gtcttctgcg gtaccatatg
     3481 ggtatcgttt agtgaatggg aaaatccaac aagaagtaca cgaagcaagg attgttcgct
     3541 atatttttca actttatttg accaaaaaat atggctacaa aaagctttgt caacgattga
     3601 cgcaacaaaa gcttttcttc cgcgaacgtc cttttcagcc ctatcatatt tatagtatcc
     3661 taaaaaatcc attgtactat ggcgaaatca aaggaggatc tttggggaag tatctaggaa
     3721 cctttgaacc gattctctcg aaagcaacct ttttacaagc ccaagagatt cgccaatcaa
     3781 ggtgtacagc taaaaaagat acgtatctgt acttattacg tcaaaaaatt cggtgtccct
     3841 tctgtggtcg tcatctgagt tcgaagtatc aatggaatac aaagaagacg aagacgcttc
     3901 attattatca ttgtaccgac cgaagctgcc aaggaatttt tcttcgggca gaaagggttg
     3961 agtcagaagt attggaaaag atacaggctt tttttcaata cgaaggcgtc tatcaagcct
     4021 tgattgccca attaacgcaa caattagccg aaaagcgaaa agatgaaaaa cgcaaagtaa
     4081 tccaacaaaa gaaacaagaa gcacaatgga ttaaggcatt tgaagagggc aattgttcac
     4141 tggaagaact caagcaacaa ctgcaagctc ttcaaaagcc gatgtctact tcttcgtttc
     4201 tttcactcca acagtgtgaa gcaaaagtga aagaatggct tcaactaaaa gacctctcta
     4261 gtcgacaatt attatgtgac tatgtttgtc ggattgaggt aacaaaggat aaaacagttt
     4321 gtgggattta cttccgacaa ctgacacaaa atattttaga aaggaacgaa tgatctatgg
     4381 aaatgataca aaccaaacga gtcgcattgt atattcgcgt gagtacacaa gaacaagctt
     4441 tagaaggaca tagtattcaa gcacaagaac aaaatggacg acagtttgcc gaacggatgg
     4501 gctatgaagt ggtagaagtt tatgccgatg aaggaatcag tgggaaatcc acaaaaaatc
     4561 ggttcgccta tcaacgaatg atgaacgatg ctcggaacca tcgttttgat ctactagtga
     4621 tttggaaatt gacccgtctc ggacgcaata tgttagatgt cttacaaaca gtcgaggaat
     4681 tattgcgata tgacgtagga ttacattcga tctcggaaca atttgacgtg accacttcga
     4741 caggtaaatt gatgttacaa ctccttggga gtttcggtga atttgaacgc aaccaaattg
     4801 cggagaatgt tcaaatgtca atgcaaagct tagtgaaaga taaaaagcgt tatgcaggag
     4861 gccgacgttt aggctatgtc agtggtattg acacgaatgg tcaaaaacaa ttggtcattg
     4921 aaccagaaga ggcgaaaatt gttcgtctga tctatgcaaa atatttaggc ggagcagggt
     4981 atcgagcgat tgccaatttt ttgaatcgtc aaggatatca aacagtcaaa aagaatgctt
     5041 tttctaccac ggcagtcaaa gatatcttac gcaataaaat ttatgcaggg tatttagaat
     5101 atgcgcgcta catcaattgg gaaaccaaac gacggaaggg gaagaatccg agaccgattt
     5161 tagtgaaagg aacccatgaa ccgattattt cggaagaaga ttatcaaaaa gtacaagaac
     5221 gccttgcttt agaaggaaaa caacccaaat ggaaccatac aggtgaaaat gtattgacag
     5281 gcttgttacg ttgtccagaa tgtggcgcac cgatggcagc gagtaatgtc acaaataccc
     5341 taaaggatgg cacaaagaaa cggattcgat attattcttg ttcagtcttt cggaataaag
     5401 gagcaagtgt gtgtcatgcc aattcgattc gtgccgaaca agcggaaaca tttgtcgccg
     5461 accgattaaa ggagatcgtt cagctaccag aagttcttcc ccgattggtg gcagcgttaa
     5521 atgaagaaat agtgcgacaa agccaaccat tggaacaaga actcgtggtg ttactggaac
     5581 gaaaggaaga attgaagact aaaattgaaa aatgggaagc tgctttggaa gatagtcctg
     5641 aattgtttcc gatgttaaaa gaccgcttag acgaattgac tgaaaaacga cgacagttgc
     5701 acattcgaga aaatgaaatc ttagggatct ttcaacaagg agagccgatt caagtcaaag
     5761 atgtccaacg gatattgact aatttggatc gttttcttgc ccagagtgag aagaagcaaa
     5821 tcaaagcctt gtatcgtacc tttatagaga aaataacctt tgatcctaac cataaggaga
     5881 tgttggaaat tacgatgaaa ttcacgcatg ccgtggtaca acaattgaat gagcaatatc
     5941 aaatagccgt atccaaaaca aaggatacgg ctatttttgt gttaaaaaca ccctttacgt
     6001 taacgatcta gtctaggtta tatcaaacaa cgttagaggg gcgctaacgc ttctctagcg
     6061 atttgaaatc gttagagaag tagttaggga ggtccttatc ataaataggg atatttatta
     6121 atgaatattt gaaatataat tgtatataag aattaaaaag agtggggata gatatgaaaa
     6181 agatgggtaa gtgtatttta tgtgaaaaat acactgaact tactaaagaa catgtaccac
     6241 ctaaaaaatc agggaatact ggaggtaaaa agaccagaac tggtaattta gatgatttcc
     6301 ttaagagcga ttttacaaaa ggtgattttc cgaaggggat taaaaggaaa cctcaaggaa
     6361 atgtttatta ta
//
LOCUS       DAYYOA010000134         6303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000134 DAYYOA010000000
VERSION     DAYYOA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.136"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACHBKA_002501"
     CDS             334..714
                     /locus_tag="ACHBKA_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9446138.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACHBKA_002502"
     CDS             696..1610
                     /locus_tag="ACHBKA_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9446139.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACHBKA_002503"
     CDS             1855..2544
                     /locus_tag="ACHBKA_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446140.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACHBKA_002504"
     CDS             complement(2665..2751)
                     /locus_tag="ACHBKA_002504"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9446141.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACHBKA_002505"
     CDS             3427..4377
                     /locus_tag="ACHBKA_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9446142.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACHBKA_002506"
     CDS             4398..4931
                     /locus_tag="ACHBKA_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9446143.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACHBKA_002507"
     CDS             4947..5117
                     /locus_tag="ACHBKA_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446144.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACHBKA_002508"
     CDS             5567..6100
                     /locus_tag="ACHBKA_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446145.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACHBKA_002509"
     CDS             6112..>6303
                     /locus_tag="ACHBKA_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446146.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAYYOA010000135         6208 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000135 DAYYOA010000000
VERSION     DAYYOA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.137"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            809..1720
                     /locus_tag="ACHBKA_002510"
     CDS             809..1720
                     /locus_tag="ACHBKA_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFM9446147.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2207..2473
                     /locus_tag="ACHBKA_002511"
     CDS             2207..2473
                     /locus_tag="ACHBKA_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446148.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2545..2886
                     /locus_tag="ACHBKA_002512"
     CDS             2545..2886
                     /locus_tag="ACHBKA_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446149.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2941..3360
                     /gene="ssb"
                     /locus_tag="ACHBKA_002513"
     CDS             2941..3360
                     /gene="ssb"
                     /locus_tag="ACHBKA_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9446150.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3437..3733
                     /locus_tag="ACHBKA_002514"
     CDS             3437..3733
                     /locus_tag="ACHBKA_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446151.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3748..4179
                     /locus_tag="ACHBKA_002515"
     CDS             3748..4179
                     /locus_tag="ACHBKA_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9446152.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4484..4666
                     /locus_tag="ACHBKA_002516"
     CDS             4484..4666
                     /locus_tag="ACHBKA_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446153.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
     gene            5070..5558
                     /locus_tag="ACHBKA_002517"
     CDS             5070..5558
                     /locus_tag="ACHBKA_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9446154.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            5545..>6208
                     /locus_tag="ACHBKA_002518"
     CDS             5545..>6208
                     /locus_tag="ACHBKA_002518"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM9446155.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQFRMFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFL
                     IFSLIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENIS
                     ILK"
BASE COUNT         2291 a          872 c         1243 g         1802 t
ORIGIN      
        1 aaccaattga aaattgaagc aaatttcact cgaattgaat cgattttgag ctaaaatcaa
       61 tagaaaaaat aaacgtattt tgtttgaaat ccgtttattt tttttacgtt tcagtattag
      121 taaggattat ttgagggtta tttagagatt atttaaaggt tatatgtaat aagtgggggg
      181 agggctgtcg gcgcttggta tgaaaggcat tctccgactt ccaggttttt gaccattttc
      241 tctcaacgtg gaaaaaccgc cacttatgag agaaaatggt caaaaaccta agcgggaagt
      301 ctccgaataa cataccaagc gccgacagcc aacagataaa gaatacacta ctaaaaatag
      361 tagtagcata aaatttaaaa gggaaaaaat caacccctac cccagtcttg gggtctcaaa
      421 actaactccc ccctaacccc ctctttcagc ctcttttgaa agagggggag aatcaacccc
      481 acggccaacg ccccagtgtt cttggggctg tccagaacag aaacttgcga gagagtgggg
      541 gtaaaacaag tgtagaggaa acttgaagac ttgttcgaac agaaacttgc gagagagccg
      601 gagtaaaaca agtgtagagg aaacttgaag acttgttcga acagaaactt gcgagagagt
      661 tggagtaaaa caagtgtaga ggaaacttga agacttgttc gaacacaaac ttacaagaga
      721 ggggagtaaa gcagttgcag aggaatcagg aagatttgtt cgaacagaaa cttgcaagaa
      781 agcattatta aattgaaggt gatgaaatat gaagtattta ttgcatggct attttattga
      841 ttattctact cgcaatagta gtactttagc gattctatgt ttgcttcatg aattgagagt
      901 ggttactaga aaacagctat acaaactatt gaattttgac agatcaatta gtaaacaagg
      961 agtaacgaat gctttagtga gattatatga aaaacagtta atcgatcgaa aaaaatatgg
     1021 aacagaaatg tattattatt tgacgaaaga gggacatcaa agcataggtg ggtattatac
     1081 gttgccgaaa gttcctgaat ataatttgaa tcaccattta caaattaaca attatttgat
     1141 taaaggaatg gcattggcaa aagaccaccc acatttaaaa cggattattt cagaaagacg
     1201 taccgtctat gaaacgaagg atttaaatcc taagacaaaa ggaagaaaat tttttgtgcc
     1261 agattttatt tttcgttttc gagacaaaga aaatcatgag atcaattggt cctttgaaat
     1321 tgagctaacg ttgaaaacac gtcgtcgcta ccgtgaagcg atttttccga aatatattcg
     1381 tgagttgcga acacgtcctg aatcacattt aatttatgtg acacctagtg gcgcaattca
     1441 agaagaactt catgcgttta agcgtatttt tttcaagcgc gaaactgaaa aagatgcgag
     1501 tcaacaagaa ttgtttgagc ggttgcatat tttttcagct ttagagtttg aagagggatt
     1561 aaaagcatta ttagagaatg atccagcagt caattggcgg acagtagaac gagaaaaaat
     1621 ttatccgaat ggcgctgttc aaagtcgttt gaatttattt gaagaatcaa ctgaaaatga
     1681 ggaaaaacca aaagtgaagt ttacgcctga agtattgtaa aagagggtgt tcgcttcgct
     1741 ctttttttgt gccagtttgc tggcaccgtt cagacgcaag cgtctgaacc ggctcgtttt
     1801 taggaaggac taacttgttt gatttatccg ttgtcaaaga agtggacgtt cgagagtccc
     1861 taatcgtata gcagtcattc ggcgagtata acctaaaaca taacctgcca attgccaagg
     1921 gtaaaatgca cgctgcgcgc ccttggcaat cgtcagaaca tgttttcgag ccggttatgc
     1981 cgaacgaccg ctatacgaaa agggcctcta aacagaagtc cactttgatc cttgacaact
     2041 gaaacccaaa atcaaacaag caggccttcg tctaaaaacg agccgaatga agaccttggg
     2101 ggttgcaccc ccaaaccccc gtgcccccga cacactaccg aaaaggtggg ggtgggtggc
     2161 gaaaaaatca aataaaacat tctattcaag aaaggaagaa acaaccatga caactaaaaa
     2221 cgagcaactg aaagaacagg caagaaaaga gttgcaagaa agaggtttga ttattgaggg
     2281 gagctttgaa ggagattttg aaacctatat tggttgttat gcaagaccga taaacaaacc
     2341 tacagcctta gacccaatga acgaacaaga agtaaaagaa caagaaaaac atgcagtgaa
     2401 tggttttccg caagatttca ctgaatggta tgagtgggaa attaacaacg ggaagcttga
     2461 aaacttctta taaaagtcag atgacgtgca aagagaatga ttctctttgt acggtaaaaa
     2521 tataaagaaa gaaggaaaaa gaaaatggca aaaaacacac cttatcaagt aggagacatt
     2581 ttttatagta gttggggcta cgaacaaacg aacgtgactt tttggcaaat tgtgaagtta
     2641 acagaaaaaa cagcatggtt tcagcctatt caaaagaaaa cggtcgagca ttattcgtcc
     2701 atgagtggaa aagttgttcc aatgaaagac aagtttatag agtatccttt agcaaaaact
     2761 aaagacagta ttgtaaaaaa acgaattgat ccagaaacct atcctgatat gttatgtggt
     2821 tatcgtagtt gggacacgtt tcatagatat aaaaatcaag ctgtttatga aagtagttat
     2881 tattaagggg atagtttccc ctttcaaaaa taataaatag aagaatggag agaaaaaatc
     2941 atgatgaatt cagtgaattt agtaggacgg ttaacaaaag aagtggattt aaaatacaca
     3001 ccaaaaggca atgcgacagg cacatttatt ttagctgtta atcgaaatta tacgaatgca
     3061 aacggcgagc gtgaagcaga ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact
     3121 ttagcaaaat atactggaaa aggaagtttg attggcatta atggacgtct tcaaacgagg
     3181 agctatcaga atcagcaagg acagaccatg tatgttacgg aagttctagt gacagatttc
     3241 tatttgttag agagtaaaga aatcaatgag caacggtcaa aatcaatggc gaataatcaa
     3301 aataattctt caattccagg aaatgagatt caaatttcag atagttattt gcccttttaa
     3361 gtgaaaaata attttgttct atacctgatt tcttcaggta tagaacaaaa aataaataga
     3421 gatgaggcga ttttttatga aaatcattga cagttacgat caacgaacca tgcaatattt
     3481 gggctcgttt gattatacag atgaaaatat tattaattat gttgcaagtt tacctcctta
     3541 tcaaagcgtt cgcttggtag attttgcaag tgatgaattg gtattaacaa cgatgggcaa
     3601 ctttttagat tatgtcggag acaaaaaatg gttggaaaaa attttgtcta aattgattgc
     3661 gaaacaaaca ggagaagaac ccataaatgc gattcaattg ttcaatcgat atgaacaaga
     3721 aaacaatgat taaagaggag agaaaaaatg aaaagattaa aacgaagtat ttttattgtg
     3781 ctagcattga ttgattttgt tgggacaggt tgtgaagcaa ttgacgttca aaaagctccc
     3841 ttatcggaaa taaaacaagc aataacaaaa caagatgaag aacaaccgct aaatacacaa
     3901 gtagaaatag tgaatcaaaa taaaccgagt ttttctgaag aagatttttc gttagaaaat
     3961 ggaaccaggc aatttttttc agatttggat cgtttaaatc gggtaggtgt agcaagtgca
     4021 cttttgcata aaagcaggat gccaacagaa gaacgagacc gattatacat taagccaact
     4081 ggctggaaac aaaagaaaat gaagaatggc gattatcttt ataatcgttg tcatttgatt
     4141 ggctatcaac gtgcgacaag tttccccata agtatgtaat acgaaacggg aggattaatc
     4201 ttaagattaa tcacaactaa cctatttgac agttggaatg aaagctatca tgtctgctga
     4261 atcaactggt ctgatactcc tacatgcaat aaagtctact aggaagttat tgtatgaagc
     4321 taggtaaagg cggcagaaca atttctaagt gtggaatcat atgaagttgg atatgccggg
     4381 acgctataaa ttactcaggg tgagactgtt tttaattaaa ctgacgaact tgcgaatgta
     4441 cgggtctata accgacttat attcgaaaga tataagattg cgaatgcaaa gaacattatc
     4501 gaaaagtaag aatgttggtc atgaaattcg agatggaaga ctagcggtgt atctgtcaag
     4561 agttggtaaa acatgtttga ttgattcaga tgagagtggg aatgcgacaa aacgttttac
     4621 agaagaaata gagcaactag cagaactgaa tatacgatat ttttaaatac tttagcggag
     4681 aataaaaaaa tcctctacag aaaactacgg agggcaacat gtgctattgt ttcaacaata
     4741 catttttaat ttttaccata acaaaaaagt acttgaaaaa aaacagaaat atgttaaatt
     4801 aaaaatgaaa aaatggaagc ggttacttat tcttttgaat ctaagataaa aattcaaaag
     4861 aataacataa cccaaatatg attatacttg agattctaaa tatttatcga aaaggaattt
     4921 gttttaagga aaaatgattc tatggacggt tattgattta tatttaccaa atggctaata
     4981 aaatagtatt tcttatattg tatagttttt gtttttatgt gaaaacatga gcaatagaat
     5041 tgtactagta ggataaggat ggatggaaaa tgaaaaaaac tatttttgtt ttagcgattt
     5101 ttatatgttt tgggctactt ttggtaggct acttgttgtt taattctaat gaaatgagaa
     5161 ctatttctac gaatgacttg ttaaaaaaaa gtgaattaaa ggataaaaaa aaagaatctc
     5221 ttgcctttat aatgtttact caagaagagt gtgagtattg tatagcaata aatccaatca
     5281 tagatagtgt aaataaggaa actttgaata agataaagaa atatgatata tctgtagatt
     5341 ttcaatctaa acaaaagtgt aaaattaagt ttgttccgac tattgttgca tatagtaaga
     5401 ataaagaagt ggggagaatt gacaatgaat tagataattt gtatcgaaaa aagcaagatg
     5461 gttcaattgt aatagacaag aaaaaaacaa agacattgct tatagattgg atgaaaaaaa
     5521 tggataaaca aggagaatat ttgtatggag aagattaaac agacatttcc gttaattaat
     5581 aaatattgga ataagaaaaa aatttttata ttttttatta cgtttctgct gtcatcttta
     5641 ttttctctaa ttttcccgta tctatttagt aatgttattg atttatattt agagaatgag
     5701 ttatcacaga atattgtgcg aatagtaacg attcttatat ttcagttatt tttagtctta
     5761 attaaagaag tgtatggata tataaaaact atttatgcat ttgaagttga tttatcaatg
     5821 aaaattgatt ttcttaaggt tttacaaaaa aaaaatcttt ttttttcgca aacgaagcat
     5881 tcaggagaaa ttcaatttag gatgttttcg gatatttcta ctatcgtaaa tactatcttt
     5941 attttttctg ttgaattacc tgtgataggc gttttagttc ttgtagcggt atttattata
     6001 agtaaagtga atcttatttt tctgattttt tcactaataa tgatgggagc tatggtgtta
     6061 cagtataatt tatttcaatt accgcttaag aaaacaatcg agaatacaaa aagtgttaat
     6121 caaagtatta caggagaatt gaatgcacat tttgatcaat tagaaattat aagcgcatat
     6181 aattttgaga atatctctat tttaaaat
//
LOCUS       DAYYOA010000136         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000136 DAYYOA010000000
VERSION     DAYYOA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.138"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHBKA_002519"
     CDS             725..811
                     /locus_tag="ACHBKA_002519"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9446156.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHBKA_002520"
     CDS             complement(932..1621)
                     /locus_tag="ACHBKA_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446157.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHBKA_002521"
     CDS             complement(1628..1906)
                     /locus_tag="ACHBKA_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446158.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHBKA_002522"
     CDS             complement(1866..2780)
                     /locus_tag="ACHBKA_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9446159.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHBKA_002523"
     CDS             complement(2762..3142)
                     /locus_tag="ACHBKA_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9446160.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHBKA_002524"
     CDS             complement(3592..3879)
                     /locus_tag="ACHBKA_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9446161.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHBKA_002525"
     CDS             complement(3899..4123)
                     /locus_tag="ACHBKA_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9446162.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHBKA_002526"
     CDS             complement(4578..4748)
                     /locus_tag="ACHBKA_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446163.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHBKA_002527"
     CDS             complement(4763..5296)
                     /locus_tag="ACHBKA_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9446164.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHBKA_002528"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHBKA_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9446165.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYOA010000137         6025 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000137 DAYYOA010000000
VERSION     DAYYOA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.139"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1093
                     /locus_tag="ACHBKA_002529"
     CDS             86..1093
                     /locus_tag="ACHBKA_002529"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM9446166.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1105..2109
                     /gene="neuB"
                     /locus_tag="ACHBKA_002530"
     CDS             1105..2109
                     /gene="neuB"
                     /locus_tag="ACHBKA_002530"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM9446167.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2106..3254
                     /gene="neuC"
                     /locus_tag="ACHBKA_002531"
     CDS             2106..3254
                     /gene="neuC"
                     /locus_tag="ACHBKA_002531"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM9446168.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3227..3904
                     /locus_tag="ACHBKA_002532"
     CDS             3227..3904
                     /locus_tag="ACHBKA_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM9446169.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3855..5036
                     /locus_tag="ACHBKA_002533"
     CDS             3855..5036
                     /locus_tag="ACHBKA_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9446170.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5049..>6025
                     /locus_tag="ACHBKA_002534"
     CDS             5049..>6025
                     /locus_tag="ACHBKA_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9446171.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         2242 a          762 c         1160 g         1861 t
ORIGIN      
        1 attattgata attagtttag ttatatattt tttaaagatt aagaatcgaa ttatagatga
       61 ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg
      121 aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat
      181 cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca
      241 cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg
      301 tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata
      361 taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc
      421 gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac
      481 taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta
      541 taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag
      601 tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac
      661 agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc
      721 tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat
      781 tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag
      841 aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac
      901 tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta
      961 tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc
     1021 agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt
     1081 cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt
     1141 gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt
     1201 ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct
     1261 ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta
     1321 aaaaaattag aattatctca atctgatttt attagaataa aagattattg tatacaaaat
     1381 aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt
     1441 ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa
     1501 atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc
     1561 agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat
     1621 tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc
     1681 gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt
     1741 gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca
     1801 gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac
     1861 actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa
     1921 gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca
     1981 gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa
     2041 tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat
     2101 gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac
     2161 ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc
     2221 atttatccca taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta
     2281 gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg
     2341 cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat
     2401 tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca
     2461 aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg
     2521 ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa
     2581 taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta
     2641 ttttaaataa tagattacct agttttagag atataaaaga aaaatatcat ctaaaatttg
     2701 aaaagaaaaa atacgttttg caagtatttc atccagttgt aaccgaacta gaaaatatag
     2761 aaaaacaaat tttagctttg atcaattcaa tggaacaaca atctcaacca attatttgca
     2821 ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa
     2881 aaacggatat tatttatatt acgaatttag agccggagga atacctgact atattaaaag
     2941 agtgcaaatt tatgatcgga aattcttctt ctgggataat tgaagctgca tcattcaaaa
     3001 taccagtaat aaatttagga accagacaaa atggacgaga acaatctaaa aatattataa
     3061 atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt
     3121 ttataaaaag cttgaaaaat gttaagaaca tctacggtga cggcaacagt tctagaagaa
     3181 taacaaaaac attgaatcaa atattgaatg atggggaaga tattaagtgg attcaaaaac
     3241 aaatagctta ttaaaatata ctagtgatcc atacattatt attggggctg gcggacacgc
     3301 aaaagtcatt attgaaacct taagattaga aaataagtta atatatggat tttttgatga
     3361 taagcaaaat aaaatgcaag atctgaaata tcttgggaaa ataaacgaat tgaatagctg
     3421 ggcggatagc aaaaaaaaaa tctataaatt tattgtagct attggtgact gtgtagtaag
     3481 aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc
     3541 aagtgctata gtggcaaaag aaatttcaat tggttatgga actgttattt ttgcgaatgc
     3601 tgtaattaac ccagatgctg taattggaga gcatgctata ataaacacag gtagcattgt
     3661 tgagcatgat tgtagaatta ataactatgt tcatttatct ccaggtgtat gcttagcggg
     3721 tggagtacat gtaggtgtag gaactcaaat aggaattggt tctcaatgta tccagatgaa
     3781 gaaaattgga tcttggtgta tgataggcgc agggagcact attgttaagg atattccctc
     3841 acattcctta gcttatggca atccagctaa aattaagaaa gaaggaatta attttgaatt
     3901 ctagaatttt attatcaagt cctcatatgg gaggaacaga agaaaaatat gttgctaagg
     3961 cttttaaaga taattggata gcgccattag gtgaaaatgt ggataaattt gaagaaagta
     4021 ttacaaaata tactggttct aagggcggag cttctgcgtt aagttcaggt acagctgcca
     4081 tacatcttgc cttaagactg cttaatgttg gaaaaaacga tattgtcttt gctccttcat
     4141 ttacttttat agctacagta aatcctattt tatatcaagg ggctgtacca gtgttcattg
     4201 actcagaacc taatacatgg aatatgagcc ctgttgcact agagagcgca ttagaagaag
     4261 ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg
     4321 ctgattatga acgaatttta gctattgcaa accaatttaa tgttccagtg atagaagatg
     4381 cagcggaatc gttaggttct acttataagg gaatttcgtc aggcttattt gggacaattg
     4441 ggatctattc atttaacggt aataagatca tcacaacctc aggtggagga gctctagtgt
     4501 ctagtgataa aaaatatgtt gagcattcaa gatttttagc aacacaagca agagatcaag
     4561 caccgtatta tcaacatagc gaaataggtt ataattaccg tctaagcaat atttctgccg
     4621 gtatcggacg tggacaaatg gaagtattga atgatagagt agctaaacga agagaaattt
     4681 tcaatcgata taaggaagct ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg
     4741 atggcatggt gccaaatcgt tggttgtcta ctttaacgat caatgattat aattctattg
     4801 atcaagtagt taacgtaata aataaactaa atgaaaaaaa cattgaaaca agagctttgt
     4861 ggaaaccact acatttgcaa ccagtatttc aaggggtaaa aatgtattac catgataata
     4921 atcttagtgt gtcagaaaag ttatttaata gcggcttatg cttaccttct ggaagtaata
     4981 tgacagatag tgaacaagaa tttgttattg atgagtttaa aaaaatattc aagtaggtgt
     5041 gatattttat gaacatagga attattggtt taggttccat ggggaaaaga agacttagac
     5101 tcattcaaga acactttaat gatattttac tatgtggtgt tgatactaat gaggcgagac
     5161 gaaaggaagt tgaaaaggaa tttggaatac caacttataa tagtctaaca gaggcaaaaa
     5221 atttgtttga aataaattca gtttttgttt gtacatcccc tataacccat gaatttatta
     5281 taaaagaggc tttaaattta gattgtaata tctttacgga aatcaattta ttaaatgaat
     5341 attatgatga aataatcgaa gtagctaaaa agaaagatag attactttat ctatcttcta
     5401 catttttgaa gagaaaagaa attcaatata ttaagtctaa agtgacgcat aataaaaaaa
     5461 tttcttatca ataccatgta ggacagtatt tacccgactg gcatccttgg gaagattatc
     5521 gcaatttttt tgtttcaaac aaaaaaacta atgggtgtag agaaatattc gctattgaat
     5581 taccttggat tatagataca tttgggaaag ttaaatcgtt tactgtaatg acccagaaat
     5641 tatccaatct tgatattgat tattcggaca gtttcactct tattattgag catgaaactg
     5701 gtatagttgg aactttaagt gtagatgttg tttcaagagt agctaaaaga aatttaacta
     5761 taattggtga aaagatttta atagaatggg aaggtaaccc ggattctcta ttttcatgga
     5821 atgaagaaac ttctagtatg acagatattc aattgtacga tgaggttatg catgataata
     5881 attattcaca tactattatt gaagatgcat atttagagga gattaaagaa tttttcacgc
     5941 ttttggaaaa gaaaatggaa ccccaatata cttttgaaaa agataagtat attattaact
     6001 taattaacga cattgaggta aatta
//
LOCUS       DAYYOA010000138         6002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000138 DAYYOA010000000
VERSION     DAYYOA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.140"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHBKA_002535"
     CDS             <1..395
                     /locus_tag="ACHBKA_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9446172.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACHBKA_002536"
     CDS             398..1228
                     /locus_tag="ACHBKA_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM9446173.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACHBKA_002537"
     CDS             1244..2095
                     /locus_tag="ACHBKA_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9446174.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACHBKA_002538"
     CDS             2092..2451
                     /locus_tag="ACHBKA_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM9446175.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACHBKA_002539"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACHBKA_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACHBKA_002540"
     CDS             2624..2929
                     /locus_tag="ACHBKA_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446176.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACHBKA_002541"
     CDS             2929..3240
                     /locus_tag="ACHBKA_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9446177.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACHBKA_002542"
     CDS             3237..3410
                     /locus_tag="ACHBKA_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446178.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACHBKA_002543"
     CDS             3407..3823
                     /locus_tag="ACHBKA_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM9446179.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACHBKA_002544"
     CDS             3823..4491
                     /locus_tag="ACHBKA_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM9446180.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACHBKA_002545"
     CDS             4488..4865
                     /locus_tag="ACHBKA_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446181.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACHBKA_002546"
     CDS             4862..5161
                     /locus_tag="ACHBKA_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446182.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACHBKA_002547"
     CDS             5151..5309
                     /locus_tag="ACHBKA_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446183.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACHBKA_002548"
     CDS             5403..5819
                     /locus_tag="ACHBKA_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9446184.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAYYOA010000139         6002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000139 DAYYOA010000000
VERSION     DAYYOA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.141"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..883)
                     /locus_tag="ACHBKA_002549"
     CDS             complement(<1..883)
                     /locus_tag="ACHBKA_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9446185.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITL"
     gene            1132..1653
                     /locus_tag="ACHBKA_002550"
     CDS             1132..1653
                     /locus_tag="ACHBKA_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9446186.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(1839..3569)
                     /gene="spxB"
                     /locus_tag="ACHBKA_002551"
     CDS             complement(1839..3569)
                     /gene="spxB"
                     /locus_tag="ACHBKA_002551"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM9446187.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            3734..4552
                     /locus_tag="ACHBKA_002552"
     CDS             3734..4552
                     /locus_tag="ACHBKA_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM9446188.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(4655..4852)
                     /locus_tag="ACHBKA_002553"
     CDS             complement(4655..4852)
                     /locus_tag="ACHBKA_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446189.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            5021..5599
                     /locus_tag="ACHBKA_002554"
     CDS             5021..5599
                     /locus_tag="ACHBKA_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9446190.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            5694..5831
                     /locus_tag="ACHBKA_002555"
     CDS             5694..5831
                     /locus_tag="ACHBKA_002555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446191.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         1769 a         1068 c         1182 g         1983 t
ORIGIN      
        1 ctaaagtaat tttttcaagc atttttttac gtgaagttgc ttgttttgat tttgatgcat
       61 tcgcactaaa gcgggcaata aattcttgga gttctttgat ttgttcttct tttttggcat
      121 ttgcgtttgc ttgaagttta gccgctagtt gactagattc caaccaaaaa tcataatttc
      181 ctacataaag tttgatttta ccaaaatcta gatccgccat atgtgtacat actttgttca
      241 aaaagtgacg gtcatgggat acaacgatca cagtattttc aaaattgatc aaaaattctt
      301 ccagccaatt gatcgattgg atatccagac cgtttgttgg ttcgtctagc aaaagaacat
      361 caggtttacc aaatagtgtt tgtgctaata acacttttac cttttgtcca ccagttaatt
      421 cactcatttg ctgatggtgc aattcttcag ggatgttcag accttgcagt aaaacagctg
      481 cttctggttc agcttcccat ccattaagct cagcaaattc tccttctaat tcagctgctc
      541 gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat cgcatctttt tctttcagca
      601 cttcatacag acgcttgtgt cccatgatga ctgtttcaag tacagtatag tcttcatagt
      661 cgtaatggtt ctgtttcaat acggccatcc gttcatctgg tcccatagta acggaacctg
      721 tggaaggctg cagatcccct gataacactt taaggaacgt tgatttacct gcaccatttg
      781 caccaatcaa gccatagcag tttccggggg taaatttaat attcacatca tcaaatagtt
      841 tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat caattttttt cctcactttt
      901 atcattatta gttttgacaa gtcttataga attatatagg ggaaaggtaa aagattcaag
      961 gcaatcgaac attctttgtg tttcacgtga aacagaatga aaaaaataaa aataaaagcg
     1021 gagcgaaaat agctactata ggatttatta gtttgcaaca gcataatttt tgacgttatt
     1081 tataagaggg agtactataa aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa
     1141 aaaagaacca tcgactgggg ctcattgatt ttgggaattt tatttgtact tgtctcattg
     1201 ttatcatttc aagaccctgt cggaaattta gttgcaattg tagtagtctt tgcgattttt
     1261 gcctttatta aagggatatt tgaactgttt gtacggaaca gattaaaaga gctaacagga
     1321 tataaaggaa agatgccctt aataatcggg atcatcgata ttctagttgg cgtctttttc
     1381 ttattcaata ttggtgcagg agtcgcagca ttgccttttg tttttgcagt atggtttatt
     1441 gctgattcag ttttagcgtt atttactgct gacttggcaa gaggagtaag tgaaggatat
     1501 tattggttta cgatcatcgt caatatttta ggaattttat taggaatttt cttattgttt
     1561 aatccaattt cttctgcgtt gacattaagc tttttagttg gattctattt tatgttattt
     1621 ggtatcacac atatcgtcta tgcatttaga taagtaaaat caaacaaata aaaaagccgg
     1681 cttccgatta cagttttact gttgtaattg gaagccggct tttttatcat ggacaaatat
     1741 gcacgaggtg atttttaggt gtcgagacaa atttatgcaa aaatgtgaga agctatatac
     1801 acaaatttta tcttaatact cgaagcatag cgcagttgtc acaacctctt ttcatataat
     1861 tcattcaata ctggcaaatc atggacttca tatttttctt tgaatgcttg gatttcttct
     1921 tcagaatatt ttcttggatc taaatgcaga tcttctactg gcaatgggcg cttgttattg
     1981 atcttcacat cgattactac aggacgatct gattttgcag ctgcatcaaa ggcaaatcga
     2041 agttgcgagt attctgtgac tgtaaagcta tttgccccga gcgcttctcc aactttaccg
     2101 aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct
     2161 tcgatgaaac caaaagaatc attcgaaaag acaacattaa taattggaag ctgatatttg
     2221 acttgtgtaa tgatatcttg catgaccata gcaaacccgc catcaccact taaggtaaat
     2281 acttgttttt ctgggtagct tagttgtgcg gcaattcctc ctggcacacc attacccata
     2341 gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt tcatattcaa taaacgaata
     2401 gaatggatag tcgtgttacc gacatctgtg acgaaaatcg catcgtcttc agcaatacga
     2461 ttgatttctt tatagacagc ctctggacga atcggttctt cctctcgatc ttcaaaagat
     2521 ttcaaccaat ttacccattt ttctttattt tcttgatttg ctaggtacca gctatcagca
     2581 gggcgagcat cccctaactc gacaagtctt tgcaaagctg tgtttgcatc accgaggatc
     2641 gacaaagatg tttcatggcg tcgtccaaat tttgacgcat cgatatcgat ttggataaat
     2701 tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt cgctaccgac aaataagatc
     2761 agatcggctt ctgccaatgc ttcatttccg ggtttagtag cgacacgtcc agcaaaacca
     2821 agaaagtttt cataatcatc aggaataatt ccttttgcta gaacggaagc gacaacagga
     2881 actgaaaaat gttctgagaa ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca
     2941 acataaagaa ctggtttttt tgctttttca agataaggca aagcagctag caaatctttt
     3001 tcttctggta atatcacact tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca
     3061 atttcttcaa aaccaagatc aacagggata gtcacgatag ccacgccttt atgctcatag
     3121 gcggctttaa tggcttcatc tactacatgc ggaagacttt gtggtgtcat gactgtacga
     3181 ttgtatacgc taacatctgc gaaaatagga ttttcattta gttcctggaa agagttatag
     3241 ttcatggatg tagaagcaac ttgtcctaag aacgctacta gaggcacatg gtccatctgt
     3301 gcatcataca aaccattgat caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg
     3361 ccgactttcc ccgttaattt tgcatctgca gcggctgcga gtgcgccgac ttcttcatga
     3421 cgaacttgaa tgtagttgat ttcggttttt tcttgatata gcgcatccat aattgaattg
     3481 aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc agctttcaag aacttttacc
     3541 attgcgattc ctgcattgat ttttgacatg gtgtttcact cctttagcaa tgatgataca
     3601 taaatttttt gtatctagag tatagaactt tttatcgaaa atcccaacta attcgtcttt
     3661 tcacaaaaat gtagtcatgt ttttgggaat atgctagagt agtacataga tagattcaag
     3721 agaggggaat ggcatggttg aatggctatt gagacttgaa ccttggcagc aagcacttgc
     3781 tgggacagct tttacttatt ttatgacagc tcttggtgcc gggttggttt ttttctttaa
     3841 agaaatcaaa aaagaagtgc taaacttgat gttaggattt gcttctggtg tcatgattgc
     3901 tgcaagtttt tggtctttgt tagatccggc tattacaaaa gcagaagaaa atggagatat
     3961 tgcttggcta gttgtaagta ttgggtttgg tttaggcggt gtgtttttat atatggcaga
     4021 taaaacactg ccccatatgc attttggacc acaacatgaa gcagaaggat tgcctaccca
     4081 tttaaaacgg acgatcttgc ttgttttttc tattacattg cataacattc ctgaaggttt
     4141 agccgtgggg gtagcattcg gtgcagcagc aactgcggat aatccgacag ccgctatatt
     4201 agccgctgtt tcggtggcgc taggtattgg tatccaaaac ttcccagaag gagcagctgt
     4261 atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag gcctttgttt acggacaagc
     4321 ttctggaatc gttgaaccaa ttgctggagt gattggagcg ctactagtaa cgaaagtgga
     4381 gctgctgctt ccttatgcac ttgcttttgc cgcaggagca atgatttatg tcgtggtgga
     4441 agaattgatc cctgaagcac agcagacatt gtccagcaaa cgtcattttg cagtttttgg
     4501 cgtcatgagt ggatttatca ttatgatgat tttggatgtg gcattaggat aaataaaaaa
     4561 aatatatatg atatatcaaa tagggccgcg aacttatatc gcgaccctat ttttttagta
     4621 agttatatca taatgaagtg aatggtatca tacattaata atagtttttt ctatgtgtat
     4681 ttcctttgat ccagcgaaca agccagatga taagtgcgat tgggattaga actttgaccg
     4741 caaaccataa aagcccgctt aggacactga caaaaagact gatgacgaag ctagctgcaa
     4801 ttagcactaa aagaaccatg aagatattta agaacatatt tttttgacgc atacaaaacc
     4861 ctcctcttgt tcattcttta ctatagaaaa agtataacag ggattgttta aaaacacctc
     4921 caactgaaga ctgattttta cttcatctac ttaaatgatc aaacaattgc tttttctctg
     4981 gtttatcatc taaaatagag cggaaaagga ggagaatatc atgcctaaaa atcgtcttga
     5041 agcatttaca gatgctgtaa tcgcgattat catgactatt ttagttttag aacttcatga
     5101 acctaacgga gacacgtggg aggcgctcgc tgcattaggt taccgatttt ttatctatat
     5161 tgtcagtttt atcattttag cgatttattg gaacaatcat catcatttat ttcaagttgt
     5221 acataagata aatggacggg tcttatgggc gaacagtttc tttgtttttg cgctttcgct
     5281 ttttccgttt gcaacttcat ggatcagtga gtacatcaat agcctggctc cagaaatgac
     5341 ttatggtttt gttaccttga ccgctaatat tgcgtattat atcttggcaa aagaacttgt
     5401 caaagcaaat aaaggccacg agaaagcatt gaaggcgatg ttgaataatt acaagaaatc
     5461 gtatctatcg attggtttga atgtgttagg aattgtctta gggtatttag tagcgccagt
     5521 agccgtgctg atcgcaaaca tcgtgattct agctgcttgg atcatcccaa gtcgtaccat
     5581 cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa
     5641 ataaataaac gatcaaatat ccgagtgata ctagtattca aagagggatg cagatggatc
     5701 aaaaaagaat tcatgtagga ctgattacta gttttgtcac gatgcttatt tatttggatc
     5761 aaggtcattt gacttggaca gtagttggtg tcgtaggatt tttgacatta ttcacagcaa
     5821 taaaaaaata aacatcttta tgtaatctga tgttctatct atttaagaaa agcatgttta
     5881 cagcagtatg attctgttgt aaacgtgttt ttatttgtgg aaaatttaat ttttagaaaa
     5941 gtgtatcgaa aaatagatag ttagtttttc cacagtggat aacttcgtaa gagataaaaa
     6001 ta
//
LOCUS       DAYYOA010000140         5975 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000140 DAYYOA010000000
VERSION     DAYYOA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.142"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1171)
                     /locus_tag="ACHBKA_002556"
     CDS             complement(41..1171)
                     /locus_tag="ACHBKA_002556"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM9446192.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1230..1457)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1819..3021)
                     /locus_tag="ACHBKA_002557"
     CDS             complement(1819..3021)
                     /locus_tag="ACHBKA_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9446193.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3081..3284)
                     /locus_tag="ACHBKA_002558"
     CDS             complement(3081..3284)
                     /locus_tag="ACHBKA_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9446194.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3426..4493)
                     /locus_tag="ACHBKA_002559"
     CDS             complement(3426..4493)
                     /locus_tag="ACHBKA_002559"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9446195.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4551..4658)
                     /locus_tag="ACHBKA_002560"
     CDS             complement(4551..4658)
                     /locus_tag="ACHBKA_002560"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9446196.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4821..5633
                     /locus_tag="ACHBKA_002561"
     CDS             4821..5633
                     /locus_tag="ACHBKA_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9446197.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            5788..>5975
                     /locus_tag="ACHBKA_002562"
     CDS             5788..>5975
                     /locus_tag="ACHBKA_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9446198.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTK"
BASE COUNT         1775 a         1214 c          964 g         2022 t
ORIGIN      
        1 cgactttttg ggacgctttt ttttgccaag cctcacattt ttatagccaa acttcttttg
       61 cgacttgctg caataattgg attttcttcc attgttcttc ttctgttaaa tggtttcctt
      121 cttctgttga agcaaaccca cattgaggac tgatacttag attttccaat ggtacaaatt
      181 gttgtgcttc ttttattcgt tcttttattt tctccgtatc ttccaaatct ggaaacttag
      241 aagtaactaa tcctaaaaca actttacttt ctttgttact ccaatgtctt aaaggagtaa
      301 aatctcctgc tcggtcagaa tcgtattcca aaaagaaacc atcataattt gttgaaaaca
      361 actcttctgc aatgggttca tatccaccgg aaaacagcca agaagaagcg tgattcccgc
      421 ggcagatatg tgttgtgatc gtcaaatctt ctggtttgtc ttccagtgcg ttattgataa
      481 tctctgtaca aatatgggcc atttctttcg gatcaattcc tttttgaaga agtgcttctc
      541 ttttagtttg atcagctaga aacgcccaat ttgtatcatc gaattgcagg taacggcaac
      601 ctttttcata aaaagcatga atcgctgctt tgtaagtaga ggacaagtcc tcataaaaat
      661 cagatagatc aggataaatc tccttgattt ttgaagaacc atcattagat aggatctctt
      721 gtcgtaaaat catcgtggga ctagggatcg ttgcttttgc aacaaaagta tcatctgttt
      781 ccactacttc tttcagaaaa gcaaaatctt ttaaaaatgg gtgatttgga ttgaaagata
      841 atttatctac tacccgaata ttataagctg gtgctgcttt tccgtggaat gtttgattat
      901 atccagtttc tggaacatag ccttcgatac catttagttc ttctaaaaaa tcaagatgcc
      961 aaaatccacg ccgaaattca ccatctgtaa ttcctttgat gccgactgct ttttggtctt
     1021 ccactaattt ttttatttcc tggtcttcga ttgcacgaag ttcttctttt gaaagtaagc
     1081 cttcttgaac tttttttctt gcttctttga tcgattgcgg gcgtaagaaa cttccgacat
     1141 gatctactgt aaatggtcta tttttgctca tttcactcac tcttttcttt gttttttgta
     1201 aaaaaaatcc ttttactagc aaatactagt aaaaggacga ttatactcgt ggtaccacct
     1261 tttttcagaa aaaacattct ttctctcagc ctgacaataa aatcagtggt cttggtaacg
     1321 aaagactgtt cgtttttgcc tacctttcga caaaaaatcc tctaaggcca ttttcgacag
     1381 tcaacttcta gctttttaca ccaaccaaag cctctcttga accattttct gtctactttc
     1441 cttctcatcg gatactatta gaataaaatt agaatagcat gtttgaaaga aaagtcaata
     1501 gggaataaga acaaacaaaa actaatttgt tattcttagt aatttgtttg tggaatacaa
     1561 taaagctttt ctaatatgat aattgttcag tcgtcctttc atcttagatc acttccttaa
     1621 aatgatcata cccgtttcaa ccacataaaa aaggacttaa ttttgaaaaa tttcccatca
     1681 aaactaagtc ctttttatta tagttatgca aatttgctta ttttctcgct atttccgata
     1741 catcttgaac aggagcaatt cgactgaaaa cactggtgca aaagcattta tttgtcttat
     1801 ggtagaaccg tggaaaactc agactggtaa taaattgtca ttttttgaaa gaatagcgcc
     1861 ctcttgaaca ttatcaatcg cacttaccac ttgttgatct gactttgcat ataagtgacc
     1921 gtaagttcct aaagtgattt gaatattcgc atggcccaat cgttcttgaa tagatagcgg
     1981 ttgaatattc tgctcaatta gaaatgcaac atgagaatgt cgtagcgcgt gaagcttaat
     2041 cggtttgaca cccgcttttt tagcagcttc ttttaaccag ttcgcaaaag aataaggtgg
     2101 cgtaaatgtt ccgtccaact gagcaacata ttcaaaattt ccaatacgtt tttgcaagtc
     2161 ataccactgt ctgagtttta aggaaagctt cttccccaac ccaatcgttc gataagcaca
     2221 tttagttttg ggagtcgaaa agtaccaatc atcttttgta ttataaatca atgtcttctc
     2281 aatcgttatc gtgtttttct caaaatctac attcgaccaa gacaaagctt caagttctcc
     2341 aatccgaatg ccagtataaa agagcatttc atacacaatc ttccgatggt gggtttgaat
     2401 cttatcatcc ttcaattcat tcatcacttt ttgaaactca ctgtgttccc aaaactcaac
     2461 tttcttattc tcagaaggaa gcgaagaaac atcttcaacg ggatttttag caatcacccc
     2521 ctctttaaca gcccgatcca atacaatttt taggtgccct cgaatccgat tgatataaga
     2581 ttttgataac gtttcgccat taactttttt ccgtgaatta cgtgactctg tctggcttag
     2641 ttcaaataac caatcctcaa tatcgtgact tgtaatatct cgaacacgaa aatgatagaa
     2701 aaactgcata cgcttttcga aaatgaattg cgcattctca tatgtttgac ttttgacctg
     2761 tctctcgtac cattttacaa acctatcctt gtaaaactct tcgaaagtca tttgattggt
     2821 agaacacagt tgattaggaa ttgtcttgtt aaaatcatgt gtttccatgt acttgtcata
     2881 agcaattttg gcatcttttt gtgaagcata acctcgttta gtcttctgaa cctttcttcc
     2941 attttcatct atacctagat acgctcggaa gaaccatttt tttgtctttt tatcttggta
     3001 aatattttta atttttggca tcgttccatt cctccctgta cttgagaaaa gctactttaa
     3061 tagtaccgcg cttgtactgt ttattccgca acttgaatgt gaaaaagatg ttcaattact
     3121 cgggatggca ccacaccaac ttggcgatta ttataaaaat caataccttc aatccttgca
     3181 agatatgcct tgctctcttt aatcattcgt gcagcttgac taggtttaaa gcccatctct
     3241 atcaaaattg cacgatttac catgaaatca cattttgttt ccattctctc acctcctgat
     3301 tctttactcc ttttaaaata atcctcttta taatctattg tttctcattc gatggttgtg
     3361 ttatactcgt cttgtcgagg cggagtacgt tgcacaactt gttgtgtaag cagtactttg
     3421 cttttttagt agcttgtttt ctcatttgat atactttgta attttcgttc ataatactca
     3481 acctcctcct gataatactc cgggtctgcc ttcatatttt ggagaagttt gatctgtctt
     3541 ggattcattt ctccttgttc aataatcaaa ggcaacaagg aagaatgaaa cgtttgatcg
     3601 acctgtgcaa taaaaactaa tgatggtgaa acaccatgaa ttaaccaact catagttttc
     3661 tcaaaacttt gaggaattgt atctaaagaa atggtgattg cgccataatt gctaataaag
     3721 gctgcccatt tggcatcaac ttttgcacct agatcatttg gatcacggtc ataaaaacaa
     3781 attgctccat taagtaactc aaagaacagt ttggttaaat catgggtttt tgaaataatc
     3841 tttgccaagc tttgcgcttt ctcttttcga taccgtagtt caaagcgatt ctttactttt
     3901 gcctcttcta aaggaattcc tcgtcgcttc cgttgttcat ggtccttctg ataaaagcaa
     3961 aaacgacact gactcttctt tgagcctaaa tacaaggtct caccatttga ttcattgttt
     4021 atgatatccg taccgccatg agattcactc acttgaaaag tggtccacac atggcctagt
     4081 attaccttat gcttaagttc tggaatgctg agactcccca caaaatcatc taaagtaaaa
     4141 tcaatccgtg taaagttccc ctgataatct aataccttac gaaaaaactg ttgccaattt
     4201 gtttttcgtg tttttagcca tcttgctaag tgtttacagc cttgacctga aaactccatc
     4261 acagttcctt taaccgcatc gtcaatggaa taccgaatcg tgattacttg attccacacg
     4321 tattggccaa tatagccctt tggcgcataa tccagttcct caaaacgttc aatgggcatt
     4381 tcaataatct cttcaattag ctttaaatgt tgattcgaga aaaagtgaat ggttagataa
     4441 tcaatcaaaa cacggttaac tggaacgacc tcttcttgca aactatccaa cacaagattc
     4501 aatttttctt cacttaattg tctcgttcct ttttctactt tcgataataa ctatctcgaa
     4561 atccccactt tttctgctaa atatcgttgt gtccaccccc tttcttctcg agaaactcga
     4621 atggcttcac cgagatcatg ttgattaact tggttcatcg acaaacctcc ttctgctatt
     4681 tacttgtcaa agaacctatt gaacttattt taagtacatt tttattgtac gcctgtattt
     4741 attttaagtc aatatgtttt gacttttttt aagtacacac gtatactaaa tttaagaact
     4801 tatcctgagg aggagttttc atggagatag acaagcaagc agtcggcaat cgaattcggc
     4861 agattcgcca agaattaaag ttgagtatgg agaaatttgg caaactaatt ggtgatttac
     4921 cgcgaagtac agtcaataac tgggaacgtg ggatcaacct tccaaaaacc gaaacccttc
     4981 accaaatcgc agagataggt catgtcacca atgaatatct tttgtatggt gatcaagaga
     5041 atcaatacat tttagaaatg ctccaaaaaa aggcaggtaa gcttgatcct aagattgaag
     5101 gtctcatagt agacgaaatc aaacacgctg accttgttag tgaaaaagaa atggatcgaa
     5161 tgattgaatt ttttattaca aatctcattc caccaactga acaagaccag tttactttcc
     5221 aatgtatcga tgaaaaaaag cagctttatc ttggttcaac taattttggt aaggaagccc
     5281 agctctattt gcaacatgat aatcaaaatc atattctgca catgatgccc tttaccttct
     5341 ccaactttag tgtggatcgc atactcgtct atttagcaaa tcaaaaatcc tattcctatt
     5401 ttgggaaaca tttacccaag gaactaggtg agaaagccat ccttctatac tcaattaatc
     5461 aattgaccag cgatgtgcga attgccccgt tggtctattc agaagaaacc gcttcttatc
     5521 agtacacaga ggacaatcaa tatctactag aacagcaata tctttatcta ccattcgtga
     5581 tggaagtcga gaaagaacgc ctgttaaatg cagcgtatcc atcatcaaaa tgaggtgaca
     5641 aaattgataa gcacgtgaca aagaaaatac tgctaggaca ctccccaatg ggtagttgtc
     5701 acagtttcct gttctttgtt agccccctgt tagatgatgt gcaccccaaa agttagacta
     5761 gaaatctaat taaaaggggt gctttttttg cggaaatata catttgtttt taagaaaaaa
     5821 gtagtttcag actatttaaa taacgaaggc ggctacaaat atcttgcaca taaatatcaa
     5881 ataaatcgta cactggttag acattgggta aggatttata actatcatgg ttgggaaggc
     5941 ttggttggag gtggcaaaag ctacactaca aaatt
//
LOCUS       DAYYOA010000141         5964 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000141 DAYYOA010000000
VERSION     DAYYOA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.143"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      24..121
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            237..818
                     /locus_tag="ACHBKA_002563"
     CDS             237..818
                     /locus_tag="ACHBKA_002563"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM9446199.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            821..2128
                     /locus_tag="ACHBKA_002564"
     CDS             821..2128
                     /locus_tag="ACHBKA_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM9446200.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2421..2909
                     /gene="purE"
                     /locus_tag="ACHBKA_002565"
     CDS             2421..2909
                     /gene="purE"
                     /locus_tag="ACHBKA_002565"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM9446201.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2902..4026
                     /gene="purK"
                     /locus_tag="ACHBKA_002566"
     CDS             2902..4026
                     /gene="purK"
                     /locus_tag="ACHBKA_002566"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM9446202.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4039..5334
                     /gene="purB"
                     /locus_tag="ACHBKA_002567"
     CDS             4039..5334
                     /gene="purB"
                     /locus_tag="ACHBKA_002567"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM9446203.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5514..5891)
                     /locus_tag="ACHBKA_002568"
     CDS             complement(5514..5891)
                     /locus_tag="ACHBKA_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446204.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT         1853 a         1023 c         1356 g         1732 t
ORIGIN      
        1 gcaagaaaat gaatttacac tattattagt tttacatacc tacttatata tcgtcataat
       61 atggatgaca gtttctagcc agtaccgtaa atgctggact ataagtaaaa gattggctat
      121 ttcacccttc ttttcttatg ataaagtgga gggtgttttt ctttaacaga aacatagtag
      181 gtttttgcac aaaagcggtt atttcggtat taataaatga taaaggagca aataaagtga
      241 aagaattagt tgaacgtatt caaaaagacg gacgtatatt aggcgagggc gtattaaaag
      301 tcgacagctt cgtcactcac caagtagacc ctgtattgat ggaacaaatg ggaaaacggt
      361 ttgcagaagt atttaaagaa caaggaatca cgaaagttgt gaccattgaa gcttctggga
      421 tcgcaccagc tttatttgct gcaagagaat tagatgttcc aatgatcttc gctagaaaag
      481 caaaaagttt gacgatggat gaagaattat tgacagcttc tgtttattct tttacgaaac
      541 aagtgacaag tacgatttct atttctcgta aatttttatc ttcagaagac aaagtattga
      601 ttatcgacga tttcttggca aacggacaag cggcaaaagg attgatcgaa ttatgccagc
      661 aagctggagc gcaagtagaa ggaatcggga tcgtcatcga aaagtctttc caagatggac
      721 gtcagttact tgaagaaatg ggattgagag tcgtttctct tgcgcgtatt gcttccttga
      781 cgaatggaca agttgagttt cttgaggagg acgcataatc gtggaaaaag aaacacaaaa
      841 tggaaaagct gcggttttag ggctgcagca tctgctagcg atgtatgcag gagcagtagc
      901 ggtgcctctt ttgatcggta caggactagg ctttaatcaa gaacagatga cttatttgat
      961 ttcaattgat atctttatgt gtggattagc gacattattg caattgacag ttaaccgatt
     1021 tttcggaatt ggcttgcctg tcgttttagg atgtgcgatc caagcagtag ctccactgat
     1081 cttgatcgga accaatgaag gagtaggagc tatctacgga tctattatcg cttctgggat
     1141 ctttgttgtg ttagtttctg gtgtcttttc taaaattaaa aaactattcc ctcctatagt
     1201 aacaggaacg gttatcaccg ttattggttt gactttgatt cctgtagcaa tcgagaaaat
     1261 gggtggaggt aatgcaacag cagctgattt tggtgacaaa acaaatctgc tacttgcatt
     1321 tgtgacgatt ctcctaattg taggcgtgca aatgctggca aaagggttca ttcgttcaat
     1381 cgcggtattg attggtttag tcggaggaag catcttagct gctttcatgg gaatgattga
     1441 tctgagcgcg attggagatg cgccgttgtt ccatgttcca caaccctttt attttggtaa
     1501 accaactttc gacgtatggt cgattttact aatgatcatc atttccatgg tcagcatggt
     1561 agaatcaaca ggtgtatatt ttgcacttgg tgacattact gggaaaaatg ttggagaaga
     1621 agaattgaaa aagggatatc gtgcagaagg acttgccgtt atcttaggcg gtattttcaa
     1681 tacattccct tatactggct tttctcaaaa tgtcggattg gttcaacttt cgggaatcaa
     1741 aacaagaaaa cccatctatt tctctgcatt cttcttgatt gttttaggat tattccctaa
     1801 aatcggtgcg ttagcacaaa tcatccctga gccggtactt ggtggaggaa tgttagtcat
     1861 gttcgggatg gtggcagtcc aaggaatccg tatgctgttg gaagttgatt tcagcaatga
     1921 taaaaatcta ttgattgttg ctgtttctgt tggatttggg ctgggtttca atgttacgcc
     1981 gacattattc agtcaaatgc cgcaaacatt gcaaatgttc actggaaatg gcatcgtaat
     2041 gagtagcatt acggctatca tattgaattt gatctttaat ggattgaaaa aagatgaaga
     2101 aatattggaa gaagccttca agaagtaata gagcaaaaga aaggttgtga aatcagcgtt
     2161 ttgacctgat tgttagagaa acaaacggaa aaatagttcc tcgtattttg ccgtttgttt
     2221 gagctaatac cgagggtcag ctgattgaac accgtttatc cataaaaagg aatgtgacct
     2281 aacttttgtc acattccttt ttttcatctt taataaacac gaacaataaa atgtttttct
     2341 ttgttaacat tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat atgaatgtag
     2401 aaaaactggg aggaaaaaca atgacagcaa aaatctctgt gattatgggg agtacttcag
     2461 attgggaaac aatgaaagaa gcatgtcaaa tattggatga attcggtgtg tcttatgaaa
     2521 aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt tgaatatgca caaaacgctc
     2581 gaaaaaaagg aatcaaaatc attattgcag gagcaggggg agccgcgcat ctgccgggta
     2641 tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc ggtccaatca cgaacattaa
     2701 atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct gtagctacta
     2761 cagcaatcgg aaaagcggga gcaattaatg ctggactact tgcgatacag atgctttcca
     2821 tgtatgattt agaattggag gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg
     2881 ttatagaaag tagtgatcaa cttggttaaa cctttgttgc ctggttcaac gatcgggatc
     2941 gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg caaaagaaat gggctttaaa
     3001 gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac aagttgcaga ttggcacatt
     3061 gaaagtgtct atgatgacac ctttgcgctg aaagagcttg ccagaagaac agatgtcatt
     3121 acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc catgtcattt
     3181 attcctcaag ggacagactt gcttgcgatt acgcaagacc gcctgttaga aaaatctttt
     3241 ttggaaacga ataacatcgt gattgctcct tatgcaacga tcgtcagtcc gacggatatc
     3301 caagatgcga ttgacgggat cggctatcct tgtgttttga aaaccactcg aggcggctat
     3361 gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc catggacctt
     3421 ttaagagaag gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga gatttcgatc
     3481 atggtggcag gaaatggtca aggagatttc acgacttttc cggtcgtgga aaacatccac
     3541 cacaacaata ttcttcatga aacgattgct ccagccgcga ttgatcagga tgtgatcgag
     3601 gaggctgagc gtattgcccg tgtgattgca gaagcagtga gcctttcagg tgttttaggg
     3661 gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga atgaattagc tcctcgtcct
     3721 cacaattctg gacattattc tattgaagct tgttctatga gtcagttcga tgcccatatt
     3781 cgaggtattt gtggttggcc attaggagaa aaagtacgtt tgataagcaa tgcagtaatg
     3841 gtcaatgttt tgggagaaca actgctggaa agctatcgct tgattgagaa taagccagac
     3901 tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc
     3961 actatcttga cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg
     4021 gattagagga gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg gaatatctgg
     4081 acggaagaga acaaataccg tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg
     4141 gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt
     4201 gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca
     4261 cgagcggttt ctgaaacatt gggagaagaa agaaaatggg tccattatgg tctaacaagt
     4321 acggatgtcg tagatactgc ttatggctat ctcatcaagc aagcaaatga tattttacgg
     4381 gaagacttga aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca caaatataca
     4441 gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa ctacttttgg attgaaactt
     4501 gccacatggt attcagaaat gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga
     4561 gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg caaatatccc gccatttatt
     4621 gaagagtatg tatgtaaaca attggggatc cgtccacaag aaatctctac tcaagttctc
     4681 cctcgggatc tacatgctga atatttctca gcgatggcgc ttattgctac aagtattgaa
     4741 cgttttgcaa cagagatacg cggattgcaa aaatccgaaa cacgtgaagt agaagaattt
     4801 tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc gatcggttca
     4861 gaaaatatgg ctggtctagc cagagtggtg cgcggacaca tggtcactgc ctatgaaaat
     4921 gtcacattat ggcatgaaag agatatttct cattcttcag cagaacgaat catcttgcct
     4981 gataccacga ttttattgaa ttacatgtta aatcgttttg gcaatatcgt taaaaatcta
     5041 acggtctttc ctgaaaacat gaagcggaat atgtctgcaa catttggttt gatttatagc
     5101 cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa gagaagaagc ttatgatctt
     5161 gttcagccaa aaactgcgta cgcttgggac aaccaagtgc agttccgccc gctgttagaa
     5221 gaagatccaa ccatcacatc ggtattatct aaagaagaaa tagacgatgc ctttgattat
     5281 catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga ataaagaaga
     5341 gtgagttgca aaattcaatt aaaatagaat agcaaaaata accaaaaaag aagcagatcc
     5401 gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag gaacctgatt
     5461 ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg
     5521 taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg
     5581 agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata atgatttttg
     5641 aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa
     5701 agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat
     5761 gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta
     5821 tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt
     5881 gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag ttaattctaa
     5941 tataccatca ttaagtataa atgt
//
LOCUS       DAYYOA010000142         5925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000142 DAYYOA010000000
VERSION     DAYYOA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.144"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1170)
                     /locus_tag="ACHBKA_002569"
     CDS             complement(76..1170)
                     /locus_tag="ACHBKA_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM9446205.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="ACHBKA_002570"
     CDS             1359..2120
                     /locus_tag="ACHBKA_002570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9446206.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACHBKA_002571"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACHBKA_002571"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9446207.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="ACHBKA_002572"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="ACHBKA_002572"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM9446208.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4812)
                     /locus_tag="ACHBKA_002573"
     CDS             complement(3760..4812)
                     /locus_tag="ACHBKA_002573"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM9446209.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            4938..5858
                     /locus_tag="ACHBKA_002574"
     CDS             4938..5858
                     /locus_tag="ACHBKA_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM9446210.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DAYYOA010000143         5847 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000143 DAYYOA010000000
VERSION     DAYYOA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.145"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..411)
                     /locus_tag="ACHBKA_002575"
     CDS             complement(37..411)
                     /locus_tag="ACHBKA_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9446211.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(434..1834)
                     /locus_tag="ACHBKA_002576"
     CDS             complement(434..1834)
                     /locus_tag="ACHBKA_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM9446212.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(1838..2248)
                     /locus_tag="ACHBKA_002577"
     CDS             complement(1838..2248)
                     /locus_tag="ACHBKA_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9446213.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2266..2757)
                     /locus_tag="ACHBKA_002578"
     CDS             complement(2266..2757)
                     /locus_tag="ACHBKA_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9446214.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(2767..3558)
                     /locus_tag="ACHBKA_002579"
     CDS             complement(2767..3558)
                     /locus_tag="ACHBKA_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9446215.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3551..4324)
                     /locus_tag="ACHBKA_002580"
     CDS             complement(3551..4324)
                     /locus_tag="ACHBKA_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9446216.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4480..>5847)
                     /locus_tag="ACHBKA_002581"
     CDS             complement(4480..>5847)
                     /locus_tag="ACHBKA_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9446217.1"
                     /translation="ESDEFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDV
                     IEETSRNFLLSKFRKFRMKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVK
                     IPETHCLIVMHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSID
                     TTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQ
                     MDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNAL
                     KKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEF
                     CLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLS
                     GMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIF
                     EQTIF"
BASE COUNT         1851 a         1181 c          746 g         2069 t
ORIGIN      
        1 ttcgttcata ccaacttaga gcacttcgtt atctatttat ttcttcattt ggcttatctc
       61 tataatctca tgatttggtc cgtagaaatt aaagtaacgg attccatttt cccagaacgg
      121 taattccata atatttttat ctaataattc atatcctaaa tcttgaattg aattgaatac
      181 ctcatctatg tcatccgtat ctaatgctag atgatcaatg gctcctctac actcagtagt
      241 tttatcttcc catatttcta ttatcgttcc attcaactcg aaaaagccga ctttacttcc
      301 cacattcatt tcttcattaa tcagactaaa ccctaaggat tcataaaaga tttttgaagc
      361 tccaaaatct tttgttggaa ttccaatatg ttgtaaacca tttaatttca cttacatcat
      421 cccttcaatt gttttaaaat ccacattcat aatttattga atagtctagt ttatctacct
      481 tgattgctcc ctccgtaaca gtcatactta atgctgttcc gactttatca tacattctag
      541 tacttaaagc aacattatca atatataaaa cagctatcgt atcatcaaca attaacttta
      601 gttcatattc tttgcctatt tccaaaaata gtggtcgttg cagcccctta tcaaacattt
      661 gaggccaagg atagttcgga gtttttccga aagtaagctg attattaatc aatgaaatac
      721 gaaattcgta tccttcttga gtatcactat ctacaaataa ttttaaagca aactcctttg
      781 ttaacgatgt aatagtcaaa gttgtttcaa atagaaaata atcttcgatt ttaggaacaa
      841 ttactctttc tttttttcca aattctttct ctaacttaaa acttgaaaat ggagtgattt
      901 ctttaaaatt ctcaattatt ttttcaggca gtttaacacc aagagttttg tctgtctttt
      961 gaataatttg atgtggaaca agtgcaccgc cccaatccca gttattttga tcgttctccc
     1021 cttcttttgt tgccacccag ccagttagaa aacggccagt ttcattagtt ccgactgttc
     1081 ttgctgcata ataggctttt ccgtcgaaag cttcgtctac tggtgatttc caatctgaaa
     1141 ataatcctgc tccgactcga tatttagtct ttttatcttc acaatactct gaaaaaagta
     1201 aataccacgt tccatcaatt tcaaatatat caggcatttc aatcatgtta aattcattat
     1261 tagcccagta gtctcctttg aaattccaat cttttaaatc ttcggattca aaatagacca
     1321 atctccccgt cggctttttc ttacttgctg gttttctagc tcctagtatc aaaacgtatt
     1381 ttccatgatc ttgatcaaaa attgcaaaag gatcgcgcca atcattctta tcatatcctt
     1441 cttgaggaac aagttttgct gcttcgccat cctttttcca atgaattaaa tctttagaac
     1501 ttgcaatcat taacacttct gaagtttttc catccaactt agctttgcga ttatgtccag
     1561 tatacaaagc taaatactca ttgattcctt ggacaatact tccagcgtaa atgtattgat
     1621 caatgtcatc gttgccacca cgatgaatta cttccccgta ttcttcaaat ttcacaaaat
     1681 cagtggttgt aactagtgac cacccaaaag gatcagttaa aggtttagga tttctcgtat
     1741 ctcgttgatg aaatagataa aaagttccat ctttataaaa aggcatgcaa tccccaaacc
     1801 aaaaatcttt aggcttgtaa aaaatttttt gcatttatca ctcatcctct tcgttagtat
     1861 gtctttgcat atctgcaaga cacgcaattg tattcatatt tcttagattt tcgatgatat
     1921 catcgctttt ttttccttgt aatctcaaat tgacagcctc aatcaaaatt cctaaattca
     1981 tgccaccaag aagttgaaac ccattttgaa atacttcact tcttttagca aattcgttga
     2041 aaggtgttcc accaatcaag tcagcaaata ccataacttc ttgatttttt acttgagaaa
     2101 ctattttttc aatgttttca cggaattctt caatgcctgc atctccaagt gttaccacat
     2161 gacaatttaa attatcgcca aaaaacattt ttaatgtatc tacaaatcca atggcaatat
     2221 ttccatgaga tgtaattata aactccatcc actcatcctc tgttcttaat aaataaaatc
     2281 ctttaaaaat tcttttgaat ctctaggagt ttgtatcact gtcacttcaa ctccatcact
     2341 aattagttct ttaaaagcct ctctctcttc ttcagtaata tttatcgtcg gacggatgcg
     2401 ttctgttcca ttccttcttg ataaatttcc tacgtttaca cttgttattg gtaaaccgtt
     2461 atcaatcaaa aatttaacat cgattggaga aactgcaaca ataagaactt tttgtccttc
     2521 atatttacca gaaagaatat ttttaactgc tacttccctt gtaattattg aagttctaac
     2581 attaggtggt gcagcgaggc gaactacagc tttttttacg tcatcattgg ctacttcatt
     2641 attaataacc ataattctgt tagcactcaa ttgatttgtc cattgtgttg ctacttgtcc
     2701 atgaatcatt cgatcatcaa tacgaacatg tataatacct ttcatttttt catccatatt
     2761 caacacctac ttcaaaatat ttaacgctct taacgcaata gaaactacta agattaatag
     2821 aattacataa actggtttaa cctttcgtcc taacaaataa tacacaattg ctactacaca
     2881 tgcaggtaat aaaccaggca taacttgatc aaagatttca tttccggtca ttgttaagtc
     2941 gccttgttta aattcaaagc caacattcac ttttacaact gtggcaatga gtgcaccgac
     3001 caccattaac cctaaaattg atactgcatc agtaatagac tttaattttg tttcaatttg
     3061 agttagtagg ctcgcacccg attctttccc agcaaggtat aaaggataag cagtaatcct
     3121 taataaaatt gatgccagta tccacaaaat aattccaaca aaactacctt ccaatgccat
     3181 attagctgca attgctccaa aaatagtcca tggaataact acaaatacac tatctccaat
     3241 tcccgctaaa ggacccatta gacctgtttt cagagcactt actgcttcca aagacttatt
     3301 tttttcttct ttttcaattc caacattcat accaataatg taaggcgctg tatacggcgt
     3361 agtgttaaaa aattgtaagt gagctaaaac ggattgtttc attccttcag gatcatcttt
     3421 atatgtgtcc ttgataaaag gaagcataga atacgcataa cccaaccctt ccattttttc
     3481 ataatttaat gaagttgcac cattgagaat ccaccggaaa attacttgtt tgtcgatttt
     3541 tgtcagtttc ttattcatct tcgtcaaaac ctcctgttgc agctggtgct acttgagtat
     3601 tatttgatct atttttatat acgattagtg cgattgcagc gccaagtaac gaaatcccta
     3661 aaattggtat ttggaaataa actgccaaaa cgaaaccaac taacaaatag gcaaagtaat
     3721 cttttgtagg taaatattta agtagcattc ctatcccaac agcaggtaat agtccccctg
     3781 ctacctttaa accaccggta acccattcag gaatataatt gattgcactt cccactaacg
     3841 ttgggcctaa tatcacagtt aacaaaacag gaattccaga tacaaagctt gtattaattg
     3901 atgccaaata ttgcatccac tctaattttt gataatcttc tttttgtgcg aatttttctg
     3961 ccttttgttg aaaaaaaatg tttgttgacc actttagaac atctagttgt acaattaaca
     4021 ttgctaccgg tattgctaaa gttattccta tagaagcatc ctggttagtt gagattgcta
     4081 aataagtacc gatcaatgct gctgtttgat aatccggtac tgatgcacca ccaaaactag
     4141 taatacctag tgtcattaat tgcaaagtcc caccgatata aagtccagtt tgaatatctc
     4201 ccataataag tccggtcaag aaacctgcaa cgacaggttg ggaggttccg attttgggcc
     4261 cttccttatc ccagttaatg aaaaatccat acaacacaac taaaaatgtt tgccaaaacg
     4321 ccattttgtt ctctcctttc ttcttatact tttatataag caattactgt gccaactaac
     4381 attatccaat aaagcgggaa aatacagtaa atattaatta aatgagcgtc taaatatact
     4441 ttggacaccc tttcattttt ttagacgcta gtatgctatt tagaaaatag tctgctcaaa
     4501 aattgtatag attaactcat actctccttt gggtatttca atacagtaaa tatctttgat
     4561 ttctgaaaaa aaatcttttg atacatttaa aaaagtatca aatagttgcg ctccctcttt
     4621 caaatgttca tttgttattc catcaccaat cataattcgt tcaatcattc cagaaagatg
     4681 tagaaataga ttaattctca gaaaacttgg gattttgaca tcgtaatatt tttcatacaa
     4741 atatagtact ttttctatct cattgaatac ttttttggaa tctaaaaagc gaagcttagt
     4801 actagcgcct tcaatagtaa agagtttaac taattcattt aagcagaact cacagctatc
     4861 cgagtccata tacttattta atttttctaa ggtttgattc ccattaatta gattttctaa
     4921 atttaaacaa tcgattcctt gaatgtaaaa ttcagtggtt gaaagaacac aaattgtatt
     4981 cttaaaaatc atagtatctt tttcctttgc tattttcttt aatgcgttaa aatcaaaaat
     5041 caaaatctcc acttgattat cgagataccg ttttaaaata tccttaactt tttcagaaat
     5101 cccctcacca gagagacaag atactattac atttttcttt tgtgataatc ctttataata
     5161 ctgaacttta acttgaaacg aatcaaaatc catctggtaa aaatgactaa ttggcttttt
     5221 ttggcaaact tgaatcccac attcaatcgc taaagctgta gaaacattgt ttaaaattac
     5281 caaatcacct attacattgg attcaatttt ttcatacatt tgttccaatg atcccatatc
     5341 aactagcaga atgagtccat tcgtcgtatc tatactttta caaaaattgt tgattaggtt
     5401 aattaagtca ctagtctcac cttcaattgg catatcgaaa gcctcataaa catatgtatt
     5461 cagcaactta tttgtttccc ttgcgatcgc agaagctgtt attttaccgt gcataactat
     5521 cagacaatgg gtttctggta ttttcacata gaacttcttt acaagaaaaa gtatccattt
     5581 caacaaaagt tcttgtttac tttcttcgac ttctaaacta ctccaaatct catttgccaa
     5641 taatttttgt ttatcatttt tcatacgaaa ttttctgaac ttagacagca aaaaatttct
     5701 tgatgtttct tcaattacat caataaacat tataaataga ctgatatccg ccaagaattc
     5761 atctaataaa catagacctg tcgaatcaat aattggtgat attttattaa caacatagtt
     5821 tcttaatgct ataaattcgt cactttc
//
LOCUS       DAYYOA010000144         5733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000144 DAYYOA010000000
VERSION     DAYYOA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.146"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..290
                     /locus_tag="ACHBKA_002582"
     CDS             <1..290
                     /locus_tag="ACHBKA_002582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446218.1"
                     /translation="TKVSSINTCSSYVQLRKSRYRGTFFFAAFFGLMRSTFAGRIRYK
                     TSFFTKDINRVKETSFCCQFFHFSKNENKKFWKWRRILLAILCLVEKRWTK"
     gene            364..1875
                     /gene="gadC"
                     /locus_tag="ACHBKA_002583"
     CDS             364..1875
                     /gene="gadC"
                     /locus_tag="ACHBKA_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM9446219.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1888..3288
                     /locus_tag="ACHBKA_002584"
     CDS             1888..3288
                     /locus_tag="ACHBKA_002584"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM9446220.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3453..4893)
                     /locus_tag="ACHBKA_002585"
                     /pseudo
     CDS             complement(3453..4893)
                     /locus_tag="ACHBKA_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5286..5733
                     /locus_tag="ACHBKA_002586"
                     /pseudo
     CDS             5286..5733
                     /locus_tag="ACHBKA_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT         1837 a          920 c         1089 g         1887 t
ORIGIN      
        1 caacaaaagt atcttcgatt aatacttgct caagttatgt gcagctacga aaaagtaggt
       61 accgaggtac tttctttttc gcagcttttt ttggacttat gagaagtaca ttcgctggaa
      121 gaatacgata taaaacgagc tttttcacta aagatattaa tagagtgaaa gaaacaagct
      181 tttgttgtca gttttttcac ttttctaaaa atgaaaataa aaaattctgg aagtggagaa
      241 gaattttgct tgctatactt tgtcttgtag aaaagaggtg gacaaaataa tgcaagctca
      301 aataagcgat tttccataac cttggtagga atgacaaatt aaataggaaa gggaagtaat
      361 aatatgaata aaaaaaagtt atctctattc ggtttttttg ccctgacagc ttccatggtt
      421 ttgactgttt atgagtatcc gacctttgca acatcacaat tacatttggt tttctttctt
      481 cttttgggag gtattttatg gtttctacca gttgctttat gtgctgcaga aatggcaaca
      541 gttgaaggat ggaaagatgg tgggattttc agttgggtta gtcaaacttt aggagaacgt
      601 tttggttttg cagctatttt tttccaatgg tttcaaatta cagtaggttt tattacaatg
      661 atctacttca tactaggagc gctgtcgtat gtaattaatt ttccagagct taatacagat
      721 ccatttatca agtttattgg tgtattagtc attttctggg tactgacttt ttcacagttg
      781 ggcgggacca atcgtaccgc taaaattgct aaagcaggat ttgtcattgg aattgttatt
      841 ccttcgatca ttctctttgg tttagcagca gcctacttta tcggcggtaa tccgattcaa
      901 atggctatta atgcgaaagc ttttattcct gacttttctc agctttcaac tttagtagta
      961 tttgtatctt ttatattggc ttatatgggc gtagaagctt cagcatcaca tattaatgag
     1021 ctggaaaatc cccaaagaaa ctacccattg gcaatgtttt tactagtgat tttggccatc
     1081 gccttagatg caatcggcgg tttctcagta gcagcggtta ttccacaaca tgacttgtct
     1141 ctcagtgctg gtgtaatcca aacatttgaa acgcttattt tacattttaa cagtcattta
     1201 cattggttgg taaaagtgat tgctctcatg attgcctgtg gcgttatggg tgaagttagt
     1261 tcttgggtag ttggtccatc acgagggatg tttgcagctg ctcagcgagg tctgttacca
     1321 gttttcttcc ggaaaactaa taaacaagac gttccagttc cgttgattat gatacaagga
     1381 attatcgtta ctatttgggc agcagtctta acttttggtg gaggaggcaa caatttatca
     1441 ttcttagtag caatctcctt aactgttgtc atttatctcg taggatattt actatttttc
     1501 ctaggttact ttgtattagt ttttaagaaa aaagacttaa aacgaactta caatgtacca
     1561 ggaaaaactg ttggcaaaac gattatcggt ggaattggtt ttattttatc agtgttcgca
     1621 ttgcttatct cttttgtacc accagcttca attgcagcta gtgagcaccg aacataccaa
     1681 atcatactac ttattagctt tgtagtgact gttcttttac cttttatcat ttatgaagta
     1741 catgataaaa gtgctcacac aactattgag gaacctactc acgtgaaagc tgaagacgtt
     1801 aatcccgctg tttatccagc tgctagagga gagcaccacg ttacaaaaaa tgatgaacat
     1861 attttaaaac attaggagga attcattatg ttatacggaa aagataatca agaagaaaaa
     1921 aactatttgg aaccaatttt tggctctgca agtgaggatg ttgacttgcc taaatataag
     1981 ttaaacaaag aatccattga accacgaatt gcttatcaat tagtacaaga cgagatgttg
     2041 gatgaaggaa atgcgcgatt aaacttagct actttttgtc aaacgtatat ggaacctgaa
     2101 gcagtgaaat tgatgaccca aacgttagaa aaaaatgcaa ttgataaatc agaataccca
     2161 cgaacaacgg aaattgaaaa ccgctgtgta aatatgattg ctgatttatg gcatgctcca
     2221 aataatgaaa aattcatggg aacttcaacg atcggctctt cagaagcctg catgctgggt
     2281 ggtatggcca tgaaatttgc ttggcgtaaa cgtgctgaaa aattaggtct tgatattcaa
     2341 gcaaaaaaac ctaacctggt gatctcttct ggttaccaag tttgttggga aaaattctgt
     2401 gtatattggg atgtggaact gagagaagtc ccaatggatg aaaaacatat gtcaattaat
     2461 ctagatactg tcatggatta tgtggatgag tacacaattg gtattgtagg tattatgggt
     2521 attacttaca ctggtcgtta tgatgatatc aagggtctga atgatttagt tgaagctcac
     2581 aataaacaaa ctgactataa agtatacatt catgttgacg ctgcatcggg tggcttttat
     2641 gcaccattta ctgaacctga tctagtttgg gattttcaat tgaaaaatgt tatctcaatt
     2701 aattcttcag gtcacaaata tggtttggta tatccaggtg tgggttgggt cttatggcgt
     2761 gaccaacaat acttaccaga agaattagta tttaaagtta gttacttagg tggagaaatg
     2821 ccaactatgg ctatcaactt ctctcatagt gcagcacaac taattgggca atactacaac
     2881 tttgtacgct atggctttga tggttatcgt gatattcacc aaagaactca tgatgttgct
     2941 gtttatttag ccaaagagat cgaaaaaact ggtatttttg aaatcattaa tgatggatca
     3001 gaattgccag ttgtgtgcta taagctgaaa gaagatccca atcgcgaatg gacactatat
     3061 gatttatctg atcgtctgtt aatgaaggga tggcaagtcc cagcctaccc attacctaaa
     3121 gacttggatc aattaattat tcaacgctta gttgttcgag cagactttgg aatgaacatg
     3181 gctggtgatt atgtacaaga tatgaaccaa gcaattgaag agttgaataa agctcatatt
     3241 gtttatcata aaaaacagga tgtgaaaacg tacggcttta ctcactaaaa ttcttcagag
     3301 aaatataaaa gaagttaatt aactaataaa aggagttgtg attaccacac agctccttaa
     3361 atctatttga ataaatatat gatattcatt tgttacactc tcaatcttag ctgttgttct
     3421 attcatcatt actcttattg taagtagttc agtcaaattt gcttgttcaa ttgatcgata
     3481 acagtatgaa ttgtttgaaa agtaatcgga tatataattg ttttctcact acctaagcct
     3541 tcttggtata aagaactatc gcaaagtata atccgctgag tacaaaagaa agtgtcgtct
     3601 actatttctc taagttgttt acaaatgata ttactaatag aaaagtgttc tcccaaaatg
     3661 tcttctaact gttgtcgata gtataaatac ctaaagtctg acggagctac tatgaacact
     3721 tcaattggag atttccgttt atgctcattt aaaagactta aactaatggt tagtttacga
     3781 atagaattct cattccaacg cagaaataaa ttgttttgtt ttcgccattg ttcaagaatt
     3841 tcatgaactt ctaagtacaa agcgcgttgc tcttcattta acaagtaagt cttatcaggt
     3901 tgaaatatct gaatatcagc ccaaagtgag cggataaacg ggagaagtgc tttatcaaaa
     3961 agtatctgat tttcttcaga tatattcaga gataagataa gtttttgact gagctcaatc
     4021 accaaagggt tatctgctac atggttttga taaacaacgt tcaagtcttt ttgtaacaat
     4081 tcaaagttat cgcatagata atttctagag ttgaatagaa agtagatata aaggcattca
     4141 tcttcgtcat gacataaaga gaaagaaagt tgctctaatc ctttctgtat tagctgaaac
     4201 aagggagtat gtcgggcaac tcttttttct tcctgactaa aatatattgg gttagtctta
     4261 gatctctgca aaccgatagc gatacctaat aaaataaatt gtttttcaac tgtagggtaa
     4321 cttcttgaaa aaaaatgttt ttctacataa tcaatcaatt gttgaccgct ttgaataatt
     4381 gatttattta gctggatcaa ctctgtccga tttgtctcat agattatcgt tagtaacaaa
     4441 cttcgataat ccttttcagg aatttcaaaa tggccatcag catttaagta ctgcatttgt
     4501 ttaaaaaaat taatcaactc atttcgtaag ttgtaaactt tgcttaacga aatgaaggta
     4561 gtatcagcta tattactcca attgtgttcc cctgataaat aatgtcctag tacattgaaa
     4621 aagtcagagc gctgatagat gagttgagtg agtgccaacc tcgaaaacga agagtcgaat
     4681 gcgactgtaa agctacttct agatttctcc actgaaataa tttgctgatg gagtatgtcg
     4741 gtaatgcatt ccaagtcgtt agtgacagac agtgagtggg caaacgttca attgatctgc
     4801 tatttcgcga atctgaatgg tccctttttc gtgtaattgc tcacaaatgt aaacttgacg
     4861 aaaaatactt ttctccagat aggtttccaa catagtactc ctcctagtca ttttatacat
     4921 aactattata actctaccaa tcggaatagt cctgtaatat attgaatcaa aaatataaat
     4981 tataaaataa ttagatgggg tatttggttt aagtatccct tatatagtaa taaatttata
     5041 ggtgtgattc tcgaattaat acccgaaaat gtgagagaat ttttatggat tttttaggat
     5101 acaaattaat tatttttgct tttatcctga ttaccagtcc agtactgtcg gtatcccatt
     5161 ttgcagcaaa atcatgttga taaagtgaca agaagactga tgagatatat tatccaaatt
     5221 gagaagataa ttaatcggat tcttgagtat accctacttt ttaagaaagt agggtataca
     5281 agcgtatgaa taaagacata actttccctg tgatggacgt tcggcttgtt ccaattacca
     5341 aaatacaggc gaacgattat aatcccaatc gagtagcgtc tccggaaatg aacttattgg
     5401 agctttctat cattgaagat ggctttactc aaccattagt ttgttattac aaggaaaaag
     5461 aagagaagta tatcttggtt gatgtgcttc ataggtatcg tgttgctaag gaaagactca
     5521 acttaaaagt tgtaccggta acagttattg ataaacccat ataagaacgt atggcttcaa
     5581 caattcgaca caatcgtgca agagggaccc atgaaataag aggaatggaa attgtcatcc
     5641 aaagattagt aaaaaatgga tggtcagaca agagaatatc caaagaatta ggcatggaca
     5701 ttgaagaaat ttttcgcttt aagcaagtgt ctg
//
LOCUS       DAYYOA010000145         5668 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000145 DAYYOA010000000
VERSION     DAYYOA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.147"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..575
                     /locus_tag="ACHBKA_002587"
     CDS             <1..575
                     /locus_tag="ACHBKA_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM9446221.1"
                     /translation="GIFQMSLDRAVVLGLLEKNPAKVIGNVKKRKTTVDFWTKEEFTK
                     VISTFYIDDLYQHFGFICMWLLFMTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRA
                     KTYEFVEPETASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRH
                     IMRRHATIVGVHIIRIHGLRHSHASLLISQ"
     gene            complement(859..1944)
                     /gene="xerS"
                     /locus_tag="ACHBKA_002588"
     CDS             complement(859..1944)
                     /gene="xerS"
                     /locus_tag="ACHBKA_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM9446222.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2278..2511
                     /locus_tag="ACHBKA_002589"
     CDS             2278..2511
                     /locus_tag="ACHBKA_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM9446223.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2508..2915
                     /locus_tag="ACHBKA_002590"
     CDS             2508..2915
                     /locus_tag="ACHBKA_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9446224.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4052..4570)
                     /locus_tag="ACHBKA_002591"
     CDS             complement(4052..4570)
                     /locus_tag="ACHBKA_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM9446225.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4937..5185
                     /locus_tag="ACHBKA_002592"
     CDS             4937..5185
                     /locus_tag="ACHBKA_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446226.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1894 a          914 c          905 g         1955 t
ORIGIN      
        1 atgggatttt tcagatgtca ttagatcgag cagttgtttt gggactactt gaaaaaaatc
       61 ctgcaaaagt tattggaaat gtgaagaaaa ggaaaactac agttgatttt tggacaaaag
      121 aagaatttac aaaagttatt tcaacttttt atattgatga tttgtaccaa cattttggtt
      181 ttatttgtat gtggttactt tttatgaccg ttatgcgttt gggagaagcc actgcattaa
      241 cttggaaaga tattgatttt gaaaataaaa acttgcgtgt gaataaaaca ctctattata
      301 agagagccaa aacctatgag tttgtagaac ccgaaacggc ttctagtatt cgtacaatcg
      361 aactagacga caatacaatt acctttttaa aagcttggaa ggagcgtcaa gtttcaatag
      421 gaggcatgtc gtttgtactt tcttataatg gaacacctac tcagaaatat acaatccgtc
      481 atattatgag aagacatgca acgatcgttg gtgttcatat aatccgcata cacggattaa
      541 ggcatagtca tgcttctttg cttatatcac aataaaaaga tgcacttcga attaaagaaa
      601 ggttaaaaca tgctgatgta caaacgactt taggaactta tggtcatttg tatctaaatt
      661 cattgagaga gataacaaat gatcccacag attatatcca aactaatttt tctaaaatca
      721 aaccgacacc tatttacgat aataaaaaat aaatgataaa caaaagtagg atcacaccaa
      781 aagaaacaca ctagaaaaac tataatacag gcttttctag cgtgttgttt cttatttccg
      841 cctatttata cattatactc ataaagcatt taatgcatcg atcgtagccg cattgtctat
      901 atgggtatat aaagttgttg tattggtact tgaatgtccc aattgttggg cagttaatac
      961 caatgaattt gtctgttggt agagtctagt agctaatgta tgacgcagtt tgtgagggct
     1021 gacttgaatt ctaaagcctt ttgaatattt tgctaccatc cgttcgattg caataccgga
     1081 tatacgcttg gcttctcctt ggtaaattgc tagaaataaa gctttttcgt tttctgttac
     1141 tttatatcgt tctttccgaa tagctagata attagctaaa tattccattg cgaatgctgc
     1201 tatattgaca gcatctcttt tatttccttt tctagttact accacattat tcttatctaa
     1261 agataggtca tctaaattaa tatttataac ctcggagagt ctcaatccag atcctaaaat
     1321 cagtgcattg atcgccaggt ctctttctac atctcgttga taatagatca aagctcgttt
     1381 actcaaagta tttttatatt cattggcaat ataatctaga taattttgtg tatccttatc
     1441 tagaaataat tttttctcaa tggcattggc acgtgcagct aacgtagttt tatctgtgat
     1501 ggttgctact tgatccatag gattattgtc taagtatggt tttcctagag gattattgga
     1561 acttttcgaa agatatttaa atagaacctt taatgccgtg atatttctat tgatagtaga
     1621 agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct ctttttttac
     1681 attttctttt gtgcgacgtt tcaaatgaga tttgtacaag tttaggtcat aggcagttaa
     1741 ctgtcttatc gaggtgatag aagtatcagg aacgatagac tcactcagaa gccattcata
     1801 aaagagctta taatttttta gatattctaa agtggttcga ggtgattgat ctttatcctc
     1861 acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg tatctatttt
     1921 ttgatatatt tctgtatttt tcatgtcatt catcctttca ataagtaggg gagaagagta
     1981 tctctataat tggactatac caattataga atgttttgtc aattggtatg ctctaaaatt
     2041 tattaatttg tttcaaaaaa agcatataaa caggtatacg tttaataatt taaaataaat
     2101 ggtatacata aatagggaag gaaatagaag ggagcaatct aaattttttg aattttctta
     2161 attaattata tagaaagaac aatctttcta tagagttaga catgagaggg actatgcttg
     2221 atttaaaatc aacaagcgta tatactgtat atacaaagaa aaaggaggga gaagactatg
     2281 gcaattaaag aaagaaaatt tagggaagtg gggaactcag tagtattaac tatttcaaag
     2341 gagtttctta aagaaaatgg gttaaatccc ggagatacgg tttttttaga tgaagaaaaa
     2401 cttcgagcgg caattacaaa aaaacaagcc ttatctgatg aaattgattt agcaatatct
     2461 caagcattaa ctgaatatga tactactttt agagagcttg tcgataaatg agtattcatt
     2521 acctagaagc caaagatatt ataaaaatga atgtaatcca aataaaaaaa tactctccta
     2581 atgaacccct tggtgtaaag gatgcgaatg ctttagaaat gtgtgtaggg caacttcgag
     2641 cctcagcttt tggtgaagag gtttatcctt cgatttatga aaaagcagct atcctactta
     2701 tacaattgat aaagaaacat ccttttcaca atgcaaataa acgtactgct ttcttagcta
     2761 cttttgtctt tttgaaaatc aatggacagc ttttagctat tgatcaaaaa gatgctgtaa
     2821 atttagcggt atatattgct acgtatgata aggatttcga tcaactaaaa tatgaagtga
     2881 tcaacactat aaagacacat acgcagccca tttaaggagt tactgtatgt gtctttatag
     2941 ggttattagc tgctatagcg aaataattga agtaaattac aaatagctat aattcaaatc
     3001 caactatata gaaaacgtaa attttctata tagttggatt aagataacga ctatttattt
     3061 tatcatattt ttactatacc ctatactttt acttggtata tttatgaata atcgcaaatt
     3121 tacccctata ctactagttt gtaagttaga ttaataaaaa tactaaagtt atttgttctt
     3181 ttattaaata cacctacaac ttataaattg atggtattta gataacacga aagaataaaa
     3241 ataacagctg atagtaaagt ttgttgacga ttgctactca atcgaaagtg agttagtgcg
     3301 tatgaacttg cttctgaaaa cttatgaaat gaaagacctt ttacatcttg cagaaatcgg
     3361 tactgggatt aaactaagca ataaaaacta atgttcccct cattctttga acttctacta
     3421 tttgatttat ttattctgat aaatgacgat ttcaccttca ggatcttcaa atcccgtata
     3481 gaatttattt aagatctctt cagtatcagt aataggatag gcgataggcc acataccctt
     3541 tgatggttag aataaactgc tcaaaatctg tatcgtatgt tggctttagc atattaacaa
     3601 aggctatctc caaattataa accataagcg ttgaatcgtt cttaacaatt tcaattcttc
     3661 ctaacccaaa gtcagaagat ataattaccg gacttgcctt acccttcatg tgggttgttg
     3721 agattccata tttaaaggga attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa
     3781 taaaaaaatc tttcccctgc tagtctccta agtccctttg tagcggatat ggcaccataa
     3841 tctcatttta tcgaagtgag tcatggttta tctgttggcc aaccaaaatt gatacaatct
     3901 aacgatgcac cctctacggc attctagggg agtgcacttg cattcaccca ataaaaaccg
     3961 gcagttagca aagaacttat gtagtcgtat gttcttactg ttaacataca agaggtctcc
     4021 tttccacaat tgcttagcag taaacgggaa tttacaaagc taatctcttc cattttctta
     4081 tctttgttct aaaagtccca aatggagcaa ctgtatttac atgaataaat ttatatactt
     4141 cccatacagc cgttttggtt gcctcgtctg cccattttct tttgtgtgca gtgaatagat
     4201 cttcatcact cattgaatca atcatcatgt taatatcatc aatattacga ttgagaattt
     4261 cctctaattc tttcaatggt agatgtgcat aggtatttgt aaaccactta tataactctc
     4321 ctaactgatt ccatttaaat tgatcagaag gcgttttaac agataatcca tttctttcat
     4381 cattttccca ttttagaatt aaattcgtcc aaccaacttg atatgctaaa ttttcagcag
     4441 gtgttctatc cacttcctca actcttttat ccttcaattc ttcaggaata gtttcaaatt
     4501 ctaaaatata tttagtataa gtcctattta tttctttttt taattcttct ttatttttat
     4561 atgttctcat tatattacct ccataaattc ctaattgtct cctttaattc atctataatt
     4621 tcatccacga tattttaatc cgttctacaa tgcatatctg atatgcattg actgtctaac
     4681 tcatatgtga atgatttata aaatatccca acgtctcatc taaagaaatg ttgatcgtca
     4741 gcaaggagct aaaaaatacg aatatatttc ttttgtagta aaatcggact ggtttgcata
     4801 tcattttctt cgtaaatatc aaaatcatgt taactactat caattactaa taaaaactac
     4861 cactttaagc gttaagaact gttaagttta gttaagtgag gatagttcac aaaatccatt
     4921 gttaaacttc acgaaagtgg taaaactcaa aatgctttat ataggattac agcatcgctc
     4981 tttcttcgat tgcgcgttgg gtaaaaaata ttatgaagta aaaattgacg atgacacgtt
     5041 ccttacggct catcagatga aacagctaca aaaacgaaat gatcaactcg aagaaaattt
     5101 aattctaaaa aagcgattgc catattcacg cctcgctcga cgaactatta aaggtcaaga
     5161 tttacctgaa gatcatcctt tttaggaaat tgaagctttt tatgttgcac aatgcattta
     5221 taatactaca atgatgtttt caccagatat tcttctaggg tgcggtgtaa tgaaacagat
     5281 acacttgcaa aagaaaattc accgcgaatt tactaaaatt atgaataatt atgttaatct
     5341 tccgaaattt ttttccaaaa ttaagggatc aagctggtgt tatagattgt ttaaggttgg
     5401 caaaaaaata acttcttttt aaacaattat agaaaaccga tactctgtta agttggttga
     5461 cacggtgtcg atttttatga ctttcttatt ctgaaacttc actaacggac agtacctaaa
     5521 aagtctatag tatggacgtt tacgagtact attttatcaa aaatgctgct catttcaact
     5581 tatatgtttt agtgaactac ccccacttag cttggatagg ctacaattaa ctagacagaa
     5641 aaattaaggt gtgtagacta gaagaaaa
//
LOCUS       DAYYOA010000146         5653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000146 DAYYOA010000000
VERSION     DAYYOA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.148"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1156
                     /locus_tag="ACHBKA_002593"
     CDS             164..1156
                     /locus_tag="ACHBKA_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9446227.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1216..2637
                     /locus_tag="ACHBKA_002594"
     CDS             1216..2637
                     /locus_tag="ACHBKA_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM9446228.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2630..4129
                     /locus_tag="ACHBKA_002595"
     CDS             2630..4129
                     /locus_tag="ACHBKA_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM9446229.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4168..5562
                     /gene="uxaC"
                     /locus_tag="ACHBKA_002596"
     CDS             4168..5562
                     /gene="uxaC"
                     /locus_tag="ACHBKA_002596"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9446230.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1855 a         1048 c         1163 g         1587 t
ORIGIN      
        1 aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca agtaattttt
       61 aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat cgtaatatga
      121 tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga
      181 tatcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag
      241 tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc
      301 gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt
      361 ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca
      421 ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac
      481 agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca
      541 gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat
      601 caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt
      661 agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt
      721 tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc
      781 tgtcaatcta gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat
      841 ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat
      901 agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc
      961 tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt
     1021 acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga
     1081 aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc
     1141 gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac
     1201 agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct
     1261 gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt
     1321 cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa
     1381 caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga
     1441 ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc
     1501 aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat
     1561 atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat
     1621 actcccccac agagcttccc gggcaaactg acagcctttt tataccgacg tttcaaatta
     1681 aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta
     1741 aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg
     1801 ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct
     1861 cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa
     1921 gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca
     1981 ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt
     2041 aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa
     2101 ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta
     2161 ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct
     2221 gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca
     2281 ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa
     2341 gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat
     2401 cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct
     2461 tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa
     2521 gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat
     2581 caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac
     2641 cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat
     2701 tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc
     2761 gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg
     2821 ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa
     2881 tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag
     2941 tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg
     3001 gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact
     3061 gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat
     3121 tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat
     3181 tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg
     3241 tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt
     3301 gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga
     3361 agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa
     3421 tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact
     3481 tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc
     3541 agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga
     3601 ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt
     3661 ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc
     3721 tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga
     3781 aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc
     3841 ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc
     3901 gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa
     3961 aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt
     4021 agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca
     4081 aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa
     4141 cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac
     4201 gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac
     4261 tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct
     4321 tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa
     4381 aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca
     4441 aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt
     4501 attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt
     4561 ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt
     4621 tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa
     4681 ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa
     4741 ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat caccacctat
     4801 caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt
     4861 gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa
     4921 gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat
     4981 cttacagaaa cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag
     5041 ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat
     5101 accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac
     5161 cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac
     5221 tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag
     5281 ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa
     5341 tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc
     5401 ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg
     5461 gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt
     5521 agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt
     5581 aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga
     5641 agacggacca aat
//
LOCUS       DAYYOA010000147         5560 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000147 DAYYOA010000000
VERSION     DAYYOA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.150"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..765
                     /locus_tag="ACHBKA_002597"
     CDS             64..765
                     /locus_tag="ACHBKA_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM9446231.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            762..1883
                     /locus_tag="ACHBKA_002598"
     CDS             762..1883
                     /locus_tag="ACHBKA_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM9446232.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1898..3130
                     /gene="pepT"
                     /locus_tag="ACHBKA_002599"
     CDS             1898..3130
                     /gene="pepT"
                     /locus_tag="ACHBKA_002599"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM9446233.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3262..4170
                     /locus_tag="ACHBKA_002600"
     CDS             3262..4170
                     /locus_tag="ACHBKA_002600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9446234.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4205..4477
                     /locus_tag="ACHBKA_002601"
     CDS             4205..4477
                     /locus_tag="ACHBKA_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM9446235.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4488..5534
                     /locus_tag="ACHBKA_002602"
     CDS             4488..5534
                     /locus_tag="ACHBKA_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM9446236.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1802 a          968 c         1210 g         1580 t
ORIGIN      
        1 ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag aaggagtgaa
       61 gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt tgtccctgaa
      121 tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc attgatatta
      181 agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta tgaatcagca
      241 cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct tgcaaatggt
      301 ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg tatgggaggt
      361 tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag cggacaagaa
      421 cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct gcaagaaaat
      481 cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata tgaaatcatt
      541 gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat gttcgggcca
      601 tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa
      661 agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa
      721 atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc agcggacgca
      781 ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa ggagacccag
      841 tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg acattagacg
      901 ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg attgctaaac
      961 atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa gaaaaaatgt
     1021 atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac atggatatta
     1081 tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg gagagttatt
     1141 tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct gtggatcatg
     1201 cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt gattataccg
     1261 gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt gcacaaccag
     1321 cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag gttattttgc
     1381 cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct tatgaggaac
     1441 ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta ggacgagtag
     1501 gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa gaagcgttcc
     1561 aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg
     1621 cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa agagcagatg
     1681 tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc gctgggttga
     1741 ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt atcgagaaat
     1801 ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct gaaacaaata
     1861 caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc ttttgccacg
     1921 ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa cgactccatc
     1981 gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag agttaggctt
     2041 agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac caagcaatgt
     2101 tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag attttaatgc
     2161 agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga tcgtactaga
     2221 tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa attatgaagg
     2281 ccacacattg atcacaacag atggcagtac attactggga gcagatgaca aatcagggat
     2341 tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc ctcacggtac
     2401 catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata aattcgatgt
     2461 tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag gtgagttgca
     2521 gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa atgtacatcc
     2581 agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt ttcataatca
     2641 gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct tccatttgat
     2701 ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg atcatgatcg
     2761 tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt tgaatcagcg
     2821 atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca tgaaagaaat
     2881 cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat
     2941 ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct cttacatggg
     3001 tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat tcgaatttgt
     3061 ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag aattgaacac
     3121 aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg
     3181 tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc tcttttttga
     3241 agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa aagttcaggg
     3301 aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga aaagcagcca
     3361 gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga attagtcaag
     3421 aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg cacgattcat
     3481 catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt acttccaggt
     3541 ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga agaagctgct
     3601 gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc
     3661 attatctcta cactgacaat cggaatctta gcagatacag cagtttggat cagtcaagaa
     3721 gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt attgctgaag
     3781 aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg aaaatcccaa
     3841 ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga cgactcagcg
     3901 accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat tttccgattt
     3961 attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg cattacttat
     4021 ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac tcgaacggat
     4081 ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg attaacagtt
     4141 tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag ttggaagaag
     4201 agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg tgaattttta
     4261 cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct ggcgtgttcc
     4321 tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat tgatctctcg
     4381 aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt
     4441 acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg tcgtctaaag
     4501 gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt gccattattt
     4561 attatttaat caatcaatca tttgcagata tctttgccga attgctgtct accagtgttg
     4621 ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa gggcgctcaa
     4681 tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg ttcttgacct
     4741 ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg atctcggaga
     4801 tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg acagcactcc
     4861 atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg gtcatccaat
     4921 tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct ggtatccttt
     4981 tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat gttcaaatat
     5041 tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa tgggtggata
     5101 catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc caagacagga
     5161 ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg tatgtacttc
     5221 cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc atttcgttta
     5281 tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt ggttcctttg
     5341 aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca gtttcttcgc
     5401 ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt gtgtatgtga
     5461 ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa gagaataaag
     5521 aaaatcaagg gtaggtcttt tataagatct acccttttta
//
LOCUS       DAYYOA010000148         5550 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000148 DAYYOA010000000
VERSION     DAYYOA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.151"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      112..278
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            426..983
                     /locus_tag="ACHBKA_002603"
                     /pseudo
     CDS             426..983
                     /locus_tag="ACHBKA_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1041..3080
                     /locus_tag="ACHBKA_002604"
     CDS             1041..3080
                     /locus_tag="ACHBKA_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9446237.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3189..4340)
                     /locus_tag="ACHBKA_002605"
     CDS             complement(3189..4340)
                     /locus_tag="ACHBKA_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9446238.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4494..5024)
                     /locus_tag="ACHBKA_002606"
     CDS             complement(4494..5024)
                     /locus_tag="ACHBKA_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9446239.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5123..5473)
                     /locus_tag="ACHBKA_002607"
     CDS             complement(5123..5473)
                     /locus_tag="ACHBKA_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM9446240.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2004 a         1019 c         1040 g         1487 t
ORIGIN      
        1 gttatttttt tctaaaaaat attaaagatt gttaaatttc actatttcat ttcaaattcc
       61 attgcctaat cattaggaaa agtctatcat gaactcgtaa atagaatata gtaataacct
      121 tgttaggtga ggctcctata caaacatagg ctgttgccca gaaacgtcga aagtcgccaa
      181 tgggtaaaac agaaactgtt ggattaaggc tttttccaat agctaagaat gttttcttta
      241 cgttgtatag tgctaaaact cggacgatga gatgatgcta ggtcagaatt cttgttcgaa
      301 aaggtttctg accttttctt tatatttttg tgactgactt atttgatgag acaaactcgc
      361 tcctcacctg acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa agatcgattg
      421 gtggaggtcg ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa
      481 aactgatctc tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga
      541 taaatgcagc ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt
      601 tcaaatttga agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta
      661 atgaactgtt tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg
      721 tatatataaa caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat
      781 ttcgggaaga caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag
      841 ctatctctca gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga
      901 aattacttgg tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg
      961 atttcctcaa agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca
     1021 agcaagagaa ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg
     1081 cttatattgc cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc
     1141 agcactatca agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc
     1201 aagctttgaa caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa
     1261 ataaaagtgc attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg
     1321 ctgatgtcaa aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt
     1381 acagtttttc atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt
     1441 ataaaggaac attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg
     1501 tttttccaga aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag
     1561 ggaatattat agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag
     1621 gagtcgttcc aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg
     1681 cttcctcctt cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac
     1741 aacccgatta ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg
     1801 gagctaccat tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc
     1861 tgattggtta cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca
     1921 gtaatggaaa aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga
     1981 caacaggtgg gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg
     2041 aacatgaagt tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa
     2101 tagcttttga cagtctagga ggaaaagctg catcaactgt tgcgacaacg ccaaaaaccg
     2161 gtgatcttct tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga
     2221 tctcacaaga agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat
     2281 ttgcgacagg ttatgctcct ggatctacgt ttaaaatgat tacagcagca atcggtctcg
     2341 acaacggcac tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag
     2401 acagttcttg gggatctttt aaaacaacgc gtgtcagtag tgatgtatca caagtagact
     2461 taaaaactgc tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg
     2521 gtgagaaaaa atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc
     2581 caattagtat gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc
     2641 tagctgatac tggatatgga cagggcgaac ttctaattac tcctatccag caagcagcaa
     2701 tgtattctgt ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag
     2761 agacaaaaga taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc
     2821 tgagagaagt tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc
     2881 cgttggcagc gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag
     2941 agaacagttt cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt
     3001 tggaaaataa agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc
     3061 tcaaccaaaa ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta
     3121 tcgtcacagt ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt
     3181 tgatttactc acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata
     3241 agtgtactta ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga
     3301 ctttgctgtc caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca
     3361 ccgaccatca atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca
     3421 agcaatttgg tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta
     3481 aagtcagaaa aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca
     3541 tatcctaaag cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct
     3601 atcccagcaa aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac
     3661 attcctaaac aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct
     3721 gcaaatgaaa gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa
     3781 attagctgtt caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga
     3841 aaagataggc tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc
     3901 aaaattgttg atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa
     3961 atactgacag aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact
     4021 tggaatgctt tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca
     4081 acgaacatac caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct
     4141 aaaaacatta ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg
     4201 acccaattca atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct
     4261 gggataccta atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat
     4321 aaaataccaa taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc
     4381 ataacataca ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa
     4441 gatttttttg gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt
     4501 atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa
     4561 ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg
     4621 aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc
     4681 atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc
     4741 aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa
     4801 agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca
     4861 aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga
     4921 aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt
     4981 aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt
     5041 tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag
     5101 gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg
     5161 ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt
     5221 cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag
     5281 gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg
     5341 tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt
     5401 ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac
     5461 gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata
     5521 taagaaaaag agaagaaaaa gcgattattc
//
LOCUS       DAYYOA010000149         5531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000149 DAYYOA010000000
VERSION     DAYYOA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.152"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..985)
                     /locus_tag="ACHBKA_002608"
     CDS             complement(<1..985)
                     /locus_tag="ACHBKA_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFM9446241.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACHBKA_002609"
     CDS             1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACHBKA_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM9446242.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="ACHBKA_002610"
     CDS             1810..2964
                     /locus_tag="ACHBKA_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM9446243.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="ACHBKA_002611"
     CDS             3179..4147
                     /locus_tag="ACHBKA_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM9446244.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="ACHBKA_002612"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="ACHBKA_002612"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM9446245.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5531
                     /locus_tag="ACHBKA_002613"
     CDS             5177..>5531
                     /locus_tag="ACHBKA_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFM9446246.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1669 a         1078 c         1251 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc g
//
LOCUS       DAYYOA010000150         5220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000150 DAYYOA010000000
VERSION     DAYYOA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.153"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACHBKA_002614"
     CDS             complement(<1..1256)
                     /locus_tag="ACHBKA_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9446247.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="ACHBKA_002615"
     CDS             complement(1270..2487)
                     /locus_tag="ACHBKA_002615"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9446248.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="ACHBKA_002616"
     CDS             complement(2477..4072)
                     /locus_tag="ACHBKA_002616"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM9446249.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="ACHBKA_002617"
     CDS             4197..4982
                     /locus_tag="ACHBKA_002617"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFM9446250.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4975..5217
                     /locus_tag="ACHBKA_002618"
     CDS             4975..5217
                     /locus_tag="ACHBKA_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446251.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAYYOA010000151         5152 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000151 DAYYOA010000000
VERSION     DAYYOA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.154"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACHBKA_002619"
     CDS             <1..649
                     /locus_tag="ACHBKA_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446252.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACHBKA_002620"
     CDS             708..1148
                     /locus_tag="ACHBKA_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446253.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACHBKA_002621"
     CDS             1160..1828
                     /locus_tag="ACHBKA_002621"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9446254.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACHBKA_002622"
     CDS             1892..2515
                     /locus_tag="ACHBKA_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM9446255.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACHBKA_002623"
     CDS             2571..2957
                     /locus_tag="ACHBKA_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM9446256.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACHBKA_002624"
     CDS             3170..4561
                     /locus_tag="ACHBKA_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM9446257.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1707 a          861 c         1084 g         1500 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac ag
//
LOCUS       DAYYOA010000152         5061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000152 DAYYOA010000000
VERSION     DAYYOA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.155"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACHBKA_002625"
     CDS             complement(205..660)
                     /locus_tag="ACHBKA_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFM9446258.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="ACHBKA_002626"
     CDS             complement(1063..1236)
                     /locus_tag="ACHBKA_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446259.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="ACHBKA_002627"
     CDS             complement(1917..2456)
                     /locus_tag="ACHBKA_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9446260.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="ACHBKA_002628"
     CDS             complement(2472..3395)
                     /locus_tag="ACHBKA_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9446261.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="ACHBKA_002629"
     CDS             complement(4523..4678)
                     /locus_tag="ACHBKA_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446262.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="ACHBKA_002630"
     CDS             complement(4697..>5061)
                     /locus_tag="ACHBKA_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFM9446263.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DAYYOA010000153         4991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000153 DAYYOA010000000
VERSION     DAYYOA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.156"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..606)
                     /locus_tag="ACHBKA_002631"
     CDS             complement(121..606)
                     /locus_tag="ACHBKA_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM9446264.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    696..1041
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1086..1727
                     /locus_tag="ACHBKA_002632"
     CDS             1086..1727
                     /locus_tag="ACHBKA_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9446265.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1746..2381
                     /locus_tag="ACHBKA_002633"
     CDS             1746..2381
                     /locus_tag="ACHBKA_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9446266.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2399..3214
                     /locus_tag="ACHBKA_002634"
     CDS             2399..3214
                     /locus_tag="ACHBKA_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9446267.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3533..4969
                     /locus_tag="ACHBKA_002635"
     CDS             3533..4969
                     /locus_tag="ACHBKA_002635"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9446268.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         1645 a          831 c         1091 g         1424 t
ORIGIN      
        1 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
       61 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
      121 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
      181 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
      241 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
      301 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
      361 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
      421 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
      481 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
      541 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
      601 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
      661 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
      721 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
      781 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
      841 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
      901 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
      961 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
     1021 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
     1081 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
     1141 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
     1201 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
     1261 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
     1321 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
     1381 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
     1441 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
     1501 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
     1561 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
     1621 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
     1681 caatcataat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
     1741 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
     1801 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
     1861 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
     1921 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
     1981 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
     2041 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
     2101 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
     2161 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
     2221 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
     2281 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
     2341 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
     2401 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
     2461 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
     2521 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
     2581 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
     2641 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
     2701 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
     2761 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
     2821 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
     2881 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
     2941 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
     3001 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
     3061 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
     3121 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
     3181 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
     3241 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
     3301 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
     3361 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
     3421 aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt
     3481 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
     3541 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
     3601 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
     3661 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
     3721 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
     3781 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
     3841 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
     3901 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
     3961 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
     4021 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
     4081 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
     4141 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
     4201 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
     4261 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
     4321 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
     4381 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
     4441 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
     4501 aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
     4561 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
     4621 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
     4681 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
     4741 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
     4801 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
     4861 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
     4921 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
     4981 aaaacaagaa g
//
LOCUS       DAYYOA010000154         4916 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000154 DAYYOA010000000
VERSION     DAYYOA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.157"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..258)
                     /locus_tag="ACHBKA_002636"
     CDS             complement(<1..258)
                     /locus_tag="ACHBKA_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446269.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ACHBKA_002637"
     CDS             complement(274..924)
                     /locus_tag="ACHBKA_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446270.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ACHBKA_002638"
     CDS             complement(935..2107)
                     /locus_tag="ACHBKA_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9446271.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ACHBKA_002639"
     CDS             complement(2126..2998)
                     /locus_tag="ACHBKA_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9446272.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ACHBKA_002640"
     CDS             complement(3020..3940)
                     /locus_tag="ACHBKA_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446273.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ACHBKA_002641"
     CDS             complement(3940..4209)
                     /locus_tag="ACHBKA_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446274.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ACHBKA_002642"
     CDS             complement(4206..4586)
                     /locus_tag="ACHBKA_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFM9446275.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1310 a         1069 c          802 g         1735 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatac
//
LOCUS       DAYYOA010000155         4889 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000155 DAYYOA010000000
VERSION     DAYYOA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.158"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..1725)
                     /locus_tag="ACHBKA_002643"
     CDS             complement(115..1725)
                     /locus_tag="ACHBKA_002643"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM9446276.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1947..2192)
                     /locus_tag="ACHBKA_002644"
     CDS             complement(1947..2192)
                     /locus_tag="ACHBKA_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446277.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2918..4258)
                     /gene="glnA"
                     /locus_tag="ACHBKA_002645"
     CDS             complement(2918..4258)
                     /gene="glnA"
                     /locus_tag="ACHBKA_002645"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM9446278.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4286..4678)
                     /locus_tag="ACHBKA_002646"
     CDS             complement(4286..4678)
                     /locus_tag="ACHBKA_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9446279.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1439 a         1053 c          798 g         1599 t
ORIGIN      
        1 ttgctttcta tcttttttcc tacaaataat aacaaatcag aaaaagagaa aagtgacaag
       61 aaaattgctt atccccttct cttgctactt ttctctttct atcaagttca aaaattattt
      121 tgtttcgtga tctgctaatg atgctccgac aaatgctttg attaattttt gcgggcgatt
      181 tggacgagaa atcaattctg ggtggaattg gcatcccaca aagaattttt tctctggcag
      241 ctctacgatt tctactaaac ggttatctgg tgagacacct gaaaagacta gcccttgttc
      301 ttcaaacaat tgacgatatt tgttattgaa ctcatagcgg tgacggtgac gttcttgcac
      361 aacatcggct ccatcatatg cttcagccgt cttcgttcct tttttgattt tacatgggta
      421 taatccaagg cgtaaagttc cacccaaatt ctcgatgttt tcctgatcag ccattaaatc
      481 aataatattg tttgctgtat ctggattcgt ttcagctgat gacgcatctt ctaatccaac
      541 gacgttacgt gcaaattcga cacaagccat ctgcatacca agacaaatac ctaaaaacgg
      601 aacgtcattt tcgcgagcat aacgaatcgc ttcgattttc ccttctactc cgcgatcacc
      661 aaatccacca ggaaccaaga ttccatctgc gtctttcaaa cgttcttcta cattttctct
      721 cgttaattct tgtgctttga tccaatcgat ctcgatgtct gaattatagg cgaaaccaga
      781 atgtttcaat gcttcaacta ctgagatata tgcatctggt aactcgacat atttaccgac
      841 aagcgcaatc cgtacttttt tcttcaagtt caagacacgt tcttctaatt ctttccattc
      901 tgtcatgtct gcttctggtg tatctagttt taaatggtcg caaacaattt gatccattcc
      961 ttgtgcttgt aaaactaatg ggatggagta aagtgtatct acatctggcg actcgataac
     1021 tgcttctggt tcgacatcac aaaattgtgc caatttgttt ttcacgcttt gagaaacagg
     1081 ttgctctgta cgtaccacca aaatattcgg ttggatacct aaaccacgta gctcttttac
     1141 actgtgctgc gttggtttag ttttcatttc tccagcagct ttcaagtaag gaatcaatgt
     1201 tgtatggata tacataacat tatctgtacc gacatctgct ttcatttgac gaagtgcttc
     1261 aaggaatggc aaagattcta tatcccctac tgttccccct acttctgtaa tgatcacatc
     1321 tgaatcagtc attttagctg ctcgcatgat tttttctttg atctcatttg taatatgagg
     1381 aataacttga acagttgctc ccaagtattc gcccttacgt tctttacgca aaacttcaga
     1441 atagattttt cctgtcgtca cgttggagta tttgttcaaa ttgatgtcaa taaaacgttc
     1501 gtagtggccc agatccaaat ctgtttcagc accatcatct gttacgaata cttctccgtg
     1561 ctgatatggg ctcatcgtcc ccggatccac gttgatgtat ggatcaaatt tttggatagt
     1621 tacttttaag ccgcggtttt tcaataagcg tcctaaagaa gctgcaacaa tcccttttcc
     1681 gattgatgaa acgacaccgc cggtcacaaa aatatatttt gtcataagtt caaaaactcc
     1741 tttttaattt atgtgcagga aaagtctcag acaataaaca aagctcccta ttcttcaata
     1801 agaataggga gccgaaattt ctgtaaactt ctgaccctct aaaagggtgc ccaaggtgta
     1861 taatacaagc tgactaaggg aaagtcaagt actcaaatga atactatccg cttacaattt
     1921 tttctttttc gatatccttt gagaaattat ttcttaagtc ctttatgagt gcaaaaaaaa
     1981 tttaacccac ataataagct tgatataaac atagctaacg ttaaacctaa gcaaatcaga
     2041 aaatcgttac tgaaaaattt taagtgaaat aatagataaa aaaataaaac aaacattatg
     2101 atagataata tcccacctaa caaagaacta ctttttccta cttgcagcaa cagtttatat
     2161 agcttatcgt agaaacaatc tttttctttc acccttattt acctcctcta cgtaaaaaac
     2221 gtagtccaca gttctcggat ttttggcata tcttttctat taaataatat caaaaacaaa
     2281 acaattccac taattttttc aaagatgacc tttttttcaa caaaagtgcc ttgtagggat
     2341 tcattgaagg taacgaatca cataagtaaa aaataaggag atgcgacaaa agtcgtgcct
     2401 ccttatttct aaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     2461 atcaaacatg agaagctgtt tttgaaatta tttcttgtga ttcggagtta aatggtaggc
     2521 tcacaacctc tggataaacg gtattcaaaa gccagtttct cggtattaag ccaaatttat
     2581 gcaaaaatgt agaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacgtctt
     2641 tttcaaaacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagttcagcc
     2701 aattcaaaaa caagaaaagc tgtttttgct tggctattct tattacttgt tgcagggcag
     2761 ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa
     2821 aaaacggcaa aatatgagga actatttttc cgttagtttc tctaatactc aggtcaaaac
     2881 gctgatttca cagcctctaa ttcacggtat tcctgtttta ataaagttct agatattgtt
     2941 ctctttccca ctcggaaaca gtttgtctga atgccgccca ttcgagacgt tttgcttcaa
     3001 caaaatttga gaagatatgt tgtcctaatg cgtcaatcat tacattatcc ttacgcaatt
     3061 ctttgatagc attatggata gttgaaggta agtcctggat atgtgcagct tctctttctt
     3121 cctcatccat tacataaatg ttacggtcaa cagctgctgg cggtgtgatc tgattacgga
     3181 taccgtctag tcctgcttgc aacaatactg ccattgccaa gtatgggttt gcagatggat
     3241 caactgaccg caattccaaa cgtgtggata aaccgcgaga ttctggtaca cgtactaatg
     3301 gcgaacgatt acggccactc catgcgacat aaacaggcgc ttcataacct ggaacgagac
     3361 gtttataaga attgactgtt ggattgcaaa cggctgtgta ggcacgagca tgtttcagca
     3421 aaccacctaa gaaatgataa gctgtttcac ttagttccaa ttcaccgtct ttatcataga
     3481 aaacatttcc ttcatcattg aacaaagaca tattgcaatg cataccagaa ccattgatcc
     3541 caaataacgg tttcggcatg aaagtcgcgt gaagtccatg cttgcgtgca atcgttttga
     3601 caacgagctt gaaggtttgg atattatcac aagcatctac cacatctgca tatttaaagt
     3661 caatttcatg ctgtcctggc gctacttcat gatgtgaagc ttccacttca aagcctagac
     3721 tttctaattc caacacgatg tcacggcggc aattttcacc taaatctgtt ggtgcaaaat
     3781 caaaataacc acctttatca ttcaaggtcg ttgtgatccc accgtcttcg tctaatttaa
     3841 ataagaagaa ttctggttca ggtcctaaat tgaaagaggt aaaaccaagg tcgcgcatat
     3901 ctttcaatgc tcgtttcaaa ttcccgcgag gatcgccggc aaatggtgtc ccatccggat
     3961 tatagatatc acaaattaaa cgagcaactt ttccatgagc actttcccac gggaagatca
     4021 tccaagtgga gagatctgga tacaaataca tatcgctttc ttcaattcga acaaatcctt
     4081 caatcgaaga accgtcaaac atcattttat tttctaatac tttgtcgatc tggctcactg
     4141 gaacttctac atttttgatc gtacctaaga tgtctgtgaa catcaatcgt aaaaatcgta
     4201 cgttttcttc ctcaatgatt cgtttgattt cttcacctgt tatctgtttc tttaccatgg
     4261 tatccctctt ccttttttaa tttattcagt caaagaattg tctttgcaat ccttgcgact
     4321 gataatgacc tgttttgctt aacccacctt gcgccaaaat ctcatcacga aaaatttgtc
     4381 gaacgtcatg atctgtcaac ggcttcttgt tttttgcttc ttgttttttc gcctgtgcca
     4441 tttcgtatac acgtttgatc ccagccatat tcaatccctc tgaaagatag tctttaatct
     4501 caagcaacat atcaatatca ttcagcgaat acatacgtcg gtttccttcg cttcgttccg
     4561 gatgaactaa atcttgttct tcataatagc gaatttgccg agcagacaaa tcagtcagct
     4621 tcataactgt accaattgga aatacggaca tagatcggcg caattccttt tctcccatca
     4681 gacgtcccct ttcgttttat aagttggcta aagtgtacca attaaaaaaa atattgtcaa
     4741 ctgcatatgt taggttttct aacatgagat ctctattttt tataaaataa caaaaaaata
     4801 agaacacaaa aaaggtatct agttgatttt cgtaccagat accttttatt ttttattata
     4861 ctgtactatc ctttattagt gtttttatt
//
LOCUS       DAYYOA010000156         4885 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000156 DAYYOA010000000
VERSION     DAYYOA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.159"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1758)
                     /locus_tag="ACHBKA_002647"
     CDS             complement(<1..1758)
                     /locus_tag="ACHBKA_002647"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9446280.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQ"
     gene            complement(1718..3471)
                     /locus_tag="ACHBKA_002648"
                     /pseudo
     CDS             complement(1718..3471)
                     /locus_tag="ACHBKA_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3581..4150)
                     /locus_tag="ACHBKA_002649"
     CDS             complement(3581..4150)
                     /locus_tag="ACHBKA_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM9446281.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4168..>4885)
                     /locus_tag="ACHBKA_002650"
     CDS             complement(4168..>4885)
                     /locus_tag="ACHBKA_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9446282.1"
                     /translation="ALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDVK
                     VGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTES
                     VRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLF
                     LFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKD
                     IKLRVEDSTLIEQIFNDITK"
BASE COUNT         1623 a          929 c          663 g         1670 t
ORIGIN      
        1 ttgagcttct ttttcataga cgaatttctc agatagttta gttttcttta tgagattttt
       61 gtatatttct gaccttcgat acaactcatt atgagtacct tgatcaacta gttttccttg
      121 atccatcact acaattttat ttaccttttc aatagacttc aagcgatgcg atataattag
      181 tactgtctta tcttgtatta aacgatttaa actttcctga atacgttttt cattttccgc
      241 atctaaactg gcagtaattt catctaaaat taaaattggc gcatttttca aaaaagcccg
      301 tgctatggat aggcgttgtc gctctcctcc agaaagtgaa cttccgtttt ctccgaccaa
      361 tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg cagttagcta aacgagctgc
      421 ttttttcact tcctcatctg acgcttgact atttccaata cgtatatttt ccaaaatact
      481 tgtattaaac aaagttacct cttgaaaaac aatagcgatt ttgctaaaca aagaagctgg
      541 agatacacgt ttaatatcat acccatcaat taaaatacag ccttcatcat aatcatacag
      601 tctagacact aatttcaaga tactagtttt cccggaacca ctagcaccta ctaacgcagt
      661 cacttctcct tgtttagccg taaaagagat atgatctaaa attggggtat tgttatcata
      721 tgagaaagag acatctcgca actcaatgtc aaaagatttc agaggactat ctgatccctc
      781 ttggatactg gtttccttca tagctctaat tcgctgtatt ttaggagcta aataaaatat
      841 ttcaagcact gcttctttca tagaatcgaa ggaatccttt atttttattg cggctagtaa
      901 gtaaccaacg acgtagagta tagtcacttc acccgttaac agtaagtgaa cacctactag
      961 aatgactact gctaaactaa catatgagaa aatagaggat aaggccatga ctgaaaaagt
     1021 acttaattct acctttaaat gaatcctttc actctcttcc atttttttga aaagtctgtc
     1081 ttgaactttt ttagatagat taaagctatt aatctcttgc tgcaattcta tagtttcttg
     1141 aaattcttcc gagttttctc tcaaagtatc gtaatattta gtattggctt tcgtttggga
     1201 tttctttgat aacaagatta agacaaaact aaataacgtt ggcaaaataa cagctaatcc
     1261 cattttgaca ttaccaacca aaagcatcac tgaaataaaa gggaaaaaca gagccatacc
     1321 gccggcttta ggtattgcat gactcatcgc atgctcaata ccttcaacgt cagacatgat
     1381 agtttgagat aaatctgata aattatgttt tgaaaaatag gataatggta aatttgacaa
     1441 atcgtctgca atttgcactc ttaaatgcgc actttcttta taagtactat tatataagtt
     1501 ttcatactcc ctatctaaca accaatacat gaaaagcaaa gtaacaaacg atacggcaaa
     1561 ataaagccaa cggggctttg tattttctaa aactaattcg tctatcagca tcattataat
     1621 gaatgctggt accatattaa tgcagtatac aaaaaaacta gaaatagaag ctttagttaa
     1681 agcttctgcg ccctgatcac ttaaagcaaa tttgttttta taaaaacttt tcattgtcaa
     1741 gcctccaatc atttgcattc tcataattag tgaccaattg tttatacact tgatgtttag
     1801 ccattaattc attatgattt ccacgttcta ctattttccc ttctgacaag acaagaattt
     1861 catctacaga tttaatgctg gataatcgat gagctatcat aataactgtt ttttctttca
     1921 tcagatgttg aaaagccttt tgtagttcat actcattatc tggatcaata gatgcactgg
     1981 cttcatccat aataacaatc tttgcatctt ttaaaattgc tctggcaata gctatacgtt
     2041 gtttttctcc tcctgacaga taaactcctt tactaccaat taaggtatgc tctctttcgg
     2101 gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag cgcgtcaaat actttacttg
     2161 ctggcgcttg aggatttgct atagaaacgt tatcgtaaat actctgattg aatagtttcg
     2221 tatcttgaaa gacaaaagca attgtgttaa taattgtgtc ttttgaatag tgatttaaag
     2281 gcttaccacc aattttaatc tgtcccttat ccacattgta gaaacctgaa attagtttag
     2341 ctatcgtact ctttccgctt cctgattttc ctactaatgc ataaattctt ttttgttcta
     2401 aaactaagtt gaaattttct aaaacacact cttctttgta tgaaaagctg acattttcaa
     2461 atgcaatgtc acaattttta atctgtgtct cgttcccata ttttagttgg tcttgcttca
     2521 tttctaaata aagggagtct agtgtttcga ctgcatatct tgcttcaaac aagtgcatac
     2581 ttacatacat tattctcatg aaagaaacaa aaataattcc acttaaaaaa aacaacatca
     2641 acaagtcaac agctaatatc ttgggagtgg taagagaagt tataaaaaag gttataggaa
     2701 ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa aagaacaaaa ggtgttttcc
     2761 cactcaaaga atacttatat gcaaacttag cataatcgtt aattgcttta ttcaaggctt
     2821 taaatgatgt tactttagca ccaaatatct ttataacttg aattccgcgg atatattcta
     2881 ccgtttcaga gcttaacaca tctaacgatt cttgatattt ttgaataaaa tttgtattct
     2941 ctgtcatttt ctttaacaag aatacaccaa ttactaacat ggcgattaga accaatccaa
     3001 ttttaaagtt aatgaaaaag cctaaggcaa ctgataaaac aggtgttact atggcttgag
     3061 cactatctgg aatcaagtga gcaattgcca tatgtgtttt agccgcgttg tcatcaattg
     3121 tctttcgaat atatcccgaa gaatttaaat caaaaaaacg aaaacttgct tctgttaaac
     3181 cgctaattcc cttttttctc aaattagtct ctaagcgaaa cccaaggata tgagaaaata
     3241 agccagaaaa aatataaaat aaagctctaa agttaaccaa gcaacagttt gaaccgcata
     3301 actcttagct aaatattcat ctcctacaat aattagagac tgaagaaatt tgtaaatact
     3361 gtaatatcca tagactgtta atacgacaga aaaatcagaa aataaagtgg ctaaccatcc
     3421 gtagtatttc attttaggga cataactaaa taattttttg tatactttca ttttttcctc
     3481 ccccgcattc aatttcttct cttattcgtt tacaactgtt aatgctttcc gtaatagcgt
     3541 aaattagaga tgtacaaaca gataaaaggt aaactacgtt ctattttttg tattttttta
     3601 agacttttct tccaatcaat acaccaatca tagataaact tgctacaact aacactgaaa
     3661 tgattcccca tatacctacc gcatagtgga tcattgcatc cattgttgca gcagattgtc
     3721 ctttatctat ccaatcttga cgaaaacgat ctgtaaagaa ccatatagga atccactgtc
     3781 cggcagcact gcctaattgc attagagccc aagacgcaac ttgaggaatt gctcgatcat
     3841 atccaaatac agatagaata atatcagcta agatcgccat actaatcgaa ctgataaacc
     3901 aaggtaagta cgccccgccc attaacgtaa aaaataacaa taaaacaatt gtgtaaatag
     3961 taaaaattcc ttttctaggg tagttatagc taatataaac aaatatgatt ccagataata
     4021 atccaaaaat cccgggggat atgctgtgac caaacgcccc accaatacta ctaaccatta
     4081 ttgaaaaaaa gtaaattact agggaaataa ctgtagctaa accaataaaa atataatctt
     4141 ttgttttcat atatattctc ctcaattcta tttcgttatg tcattaaaaa tctgttctat
     4201 caaagttgaa tcttctactc taagttttat atcttttttt attccaccat tttttaaata
     4261 aataattcta ttgcatgttt tataaagaaa ttccggatca tgagtagcta taactactgc
     4321 acccttttct tttaagtctt taataaggtc agcaatcctt tccatattaa tataatccaa
     4381 cccacttgta ggttcatcaa atagaaataa attagttttt gacaatgaag ctatagaaac
     4441 caataatctt tgtttttctc caccggataa atcaaacggt aattgatctt tcattttata
     4501 aagttccatt ttttttaaaa ttttttcttg ctgatcttta ctaactattt cattccccag
     4561 ggctaactcg ctcctcactg attctgtgaa aaattggtaa tccatttctt gcattactaa
     4621 aaacggcgat gacaaatttt ttttattttc gattatcttt cctttggtag ggcgaataat
     4681 cttcatgagt attcttagta aggttgtttt ccccacaccg tttttcccta ccaaaccaat
     4741 aacatctcca actttgacat caaaatttat gtcttttaga atattttcat aagtaatttc
     4801 ttcaactttt aatacatctg cacttacctt ttcgatatag ggaacttcaa tcgaactatt
     4861 aaaaatgtca aatttcctta atgcg
//
LOCUS       DAYYOA010000157         4790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000157 DAYYOA010000000
VERSION     DAYYOA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.160"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(182..1144)
                     /locus_tag="ACHBKA_002651"
     CDS             complement(182..1144)
                     /locus_tag="ACHBKA_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9446283.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="ACHBKA_002652"
     CDS             complement(1146..2585)
                     /locus_tag="ACHBKA_002652"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM9446284.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="ACHBKA_002653"
     CDS             2770..4716
                     /locus_tag="ACHBKA_002653"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM9446285.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1530 a          815 c          788 g         1657 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat
//
LOCUS       DAYYOA010000158         4788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000158 DAYYOA010000000
VERSION     DAYYOA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.161"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACHBKA_002654"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACHBKA_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM9446286.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACHBKA_002655"
     CDS             1019..1183
                     /locus_tag="ACHBKA_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446287.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACHBKA_002656"
     CDS             2141..3118
                     /locus_tag="ACHBKA_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9446288.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACHBKA_002657"
     CDS             3128..3625
                     /locus_tag="ACHBKA_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9446289.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACHBKA_002658"
     CDS             4001..4282
                     /locus_tag="ACHBKA_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446290.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACHBKA_002659"
     CDS             4284..4700
                     /locus_tag="ACHBKA_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446291.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAYYOA010000159         4775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000159 DAYYOA010000000
VERSION     DAYYOA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.162"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACHBKA_002660"
     CDS             complement(<1..1128)
                     /locus_tag="ACHBKA_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM9446292.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACHBKA_002661"
     CDS             complement(1164..1874)
                     /locus_tag="ACHBKA_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9446293.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2802)
                     /locus_tag="ACHBKA_002662"
     CDS             complement(1942..2802)
                     /locus_tag="ACHBKA_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM9446294.1"
                     /translation="MFVAIWGKWSTRRIKMKVLGLGDNVVDKYENLGIMYPGGNSLNF
                     AVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGD
                     RIFLGSNSGGVTREYPIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYD
                     FSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIG
                     YDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVD
                     GSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACHBKA_002663"
     CDS             complement(2768..3736)
                     /locus_tag="ACHBKA_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9446295.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>4775
                     /locus_tag="ACHBKA_002664"
     CDS             4499..>4775
                     /locus_tag="ACHBKA_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM9446296.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT         1473 a          983 c          726 g         1593 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagt
//
LOCUS       DAYYOA010000160         4732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000160 DAYYOA010000000
VERSION     DAYYOA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.163"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1118
                     /locus_tag="ACHBKA_002665"
     CDS             72..1118
                     /locus_tag="ACHBKA_002665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9446297.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="ACHBKA_002666"
     CDS             1135..2358
                     /locus_tag="ACHBKA_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9446298.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="ACHBKA_002667"
     CDS             2447..3334
                     /locus_tag="ACHBKA_002667"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9446299.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="ACHBKA_002668"
     CDS             3420..3650
                     /locus_tag="ACHBKA_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM9446300.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="ACHBKA_002669"
     CDS             complement(3707..4672)
                     /locus_tag="ACHBKA_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9446301.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1601 a          788 c          963 g         1380 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca ag
//
LOCUS       DAYYOA010000161         4711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000161 DAYYOA010000000
VERSION     DAYYOA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.164"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..357
                     /locus_tag="ACHBKA_002670"
                     /pseudo
     CDS             106..357
                     /locus_tag="ACHBKA_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ACHBKA_002671"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ACHBKA_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM9446302.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ACHBKA_002672"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ACHBKA_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1786..1869
                     /locus_tag="ACHBKA_002673"
     CDS             1786..1869
                     /locus_tag="ACHBKA_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM9446303.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHBKA_002674"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHBKA_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9446304.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ACHBKA_002675"
     CDS             complement(2994..3857)
                     /locus_tag="ACHBKA_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9446305.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ACHBKA_002676"
     CDS             complement(3859..4131)
                     /locus_tag="ACHBKA_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9446306.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="ACHBKA_002677"
     CDS             complement(4148..4363)
                     /locus_tag="ACHBKA_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9446307.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4711)
                     /locus_tag="ACHBKA_002678"
     CDS             complement(4455..>4711)
                     /locus_tag="ACHBKA_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFM9446308.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc a
//
LOCUS       DAYYOA010000162         4610 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000162 DAYYOA010000000
VERSION     DAYYOA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.165"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..400)
                     /locus_tag="ACHBKA_002679"
     CDS             complement(86..400)
                     /locus_tag="ACHBKA_002679"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9446309.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(451..705)
                     /locus_tag="ACHBKA_002680"
     CDS             complement(451..705)
                     /locus_tag="ACHBKA_002680"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9446310.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHBKA_002681"
     CDS             complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHBKA_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM9446311.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="ACHBKA_002682"
     CDS             complement(1145..2098)
                     /locus_tag="ACHBKA_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9446312.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="ACHBKA_002683"
     CDS             2710..4203
                     /locus_tag="ACHBKA_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM9446313.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4610
                     /locus_tag="ACHBKA_002684"
     CDS             4338..>4610
                     /locus_tag="ACHBKA_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9446314.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1491 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga
//
LOCUS       DAYYOA010000163         4475 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000163 DAYYOA010000000
VERSION     DAYYOA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.166"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            326..1054
                     /locus_tag="ACHBKA_002685"
     CDS             326..1054
                     /locus_tag="ACHBKA_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9446315.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="ACHBKA_002686"
     CDS             1199..3226
                     /locus_tag="ACHBKA_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM9446316.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="ACHBKA_002687"
     CDS             3334..4431
                     /locus_tag="ACHBKA_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9446317.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DAYYOA010000164         4474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000164 DAYYOA010000000
VERSION     DAYYOA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.167"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..654)
                     /locus_tag="ACHBKA_002688"
     CDS             complement(43..654)
                     /locus_tag="ACHBKA_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9446318.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(777..902)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(984..1700)
                     /locus_tag="ACHBKA_002689"
     CDS             complement(984..1700)
                     /locus_tag="ACHBKA_002689"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9446319.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1706..2275)
                     /gene="scpB"
                     /locus_tag="ACHBKA_002690"
     CDS             complement(1706..2275)
                     /gene="scpB"
                     /locus_tag="ACHBKA_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM9446320.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2259..3029)
                     /locus_tag="ACHBKA_002691"
     CDS             complement(2259..3029)
                     /locus_tag="ACHBKA_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM9446321.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3026..3397)
                     /locus_tag="ACHBKA_002692"
     CDS             complement(3026..3397)
                     /locus_tag="ACHBKA_002692"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9446322.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3416..4303)
                     /gene="xerD"
                     /locus_tag="ACHBKA_002693"
     CDS             complement(3416..4303)
                     /gene="xerD"
                     /locus_tag="ACHBKA_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM9446323.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1214 a          980 c          784 g         1496 t
ORIGIN      
        1 tccaaaaaaa aagaccaaga aatttcttga tctttttgat atttatttga ctgctgtatg
       61 acgttgttcg aactgatgtt gttttcgact taaccatgga agaagttgcg catgcagcac
      121 taaaaagaca gcactcacga tgaatccttt gatcaagttg aaaggaacga tcccaatcag
      181 tacatagttt gctaaagaca tgcccagcat ctgtgttgcg ttcagcccta aaaacattaa
      241 gtaaagaggc gtaataacga aataatttgc gatactcata aaaatagtta gagcgatggt
      301 tcctgtaatg acacctaaaa atttgtttct tcctttatgc tgtttcttta cactaaagaa
      361 ataataaatc gggaatgtaa agatcgttgt tgataagaaa ctagcagtgt ctcctaccaa
      421 attatccgga gaaaaacctg tagtcagcag atgtaatact gaacgcaaga acgctgttgc
      481 aactccggcc gctggaccga ataagaacat gctgatcaat accgagatat cactaaaatc
      541 gatcttcaaa aaactaaaag ccggcatgat cggaaaagca aaatactgta aaactaatgc
      601 gatcgcagca aacatcgcta ctgcaaccat tttctgtaca cgggtgttct tcatttgaaa
      661 tcctcctgtt aggactcctc ttcagcgaac tttttgacgt ttctctccaa atgaaaaaac
      721 tctattttcc agggaaaata gagttagagt tatttgatca agcgtcgtca aataagctct
      781 atcttcttta tccagactat actgtcggta tcggaatttc accgattcag ctattttttt
      841 gcaaaatagg tcgtggacta taaccaccgg tcgggaattt caccctgccc ctgaagacga
      901 accttatata atttttgttt acatattcat tatacatatc tattcaaaaa acgcaacatc
      961 taacaattat aaaaagtaag tgttcatttt gagtggttca gcaaactgct gacttctttt
     1021 ttgttcagtt cacggtaatc cccaggacgt aggccttgta aagtcagatc tccgtatttt
     1081 tcacgtttta gtttttgcac aggaagacca actgcttgaa gcatattttt cacttgatga
     1141 ttacgtcctt cgtggatcgt caattccacg atactgtggt ttgtcttagg atccactgat
     1201 aaaatttgga atttagctgg agatgttttt cgtccttcga tctttattcc ttttgtcaaa
     1261 ggtgcaagca tcgattttgt agcgatccct tttacctttg caacgtaaac tttatccact
     1321 tcatgttttg gatgtgtcag gcgctgagca aaatctccat catttgtcag tagcaagatc
     1381 cccgaagtat cgtaatccag tcgacctaca ggatagatac gttcttttac catcggcaaa
     1441 agatccgtaa caacttttct tcctttgtca tctgcaacag cagaaataac tcctttaggt
     1501 ttgtacaata aatagtaacc gtattcttct tgataaattg ggacttcatc cacttccaca
     1561 gtgtcatgtt tcccaacttt tatccctaat tcccttacaa cttgtccgtt tacttttaca
     1621 cgtcctgcaa ggatgaattc ttctgctttt cgacgagaag tgatgccagc gtgggcaatt
     1681 actttctgca atctctccat tacaattatt ctcctttatt cattccatca aagaatagat
     1741 ctggtgacac gtcttcttca agtgaaattt ccatttcttg tgtatcaggc agttcttcta
     1801 atgatttcaa accaaaataa tctaagaagt agccagttgt tccatataat atcggccgac
     1861 ctggtccttc tactctacct ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg
     1921 ctcctgaaac ctgaactcct cgaatctgct cgatttcagc tcttgtgatt ggctgtttat
     1981 aagcgatgat tgctaaggtt tcgacggcgg cttgagataa tgtgtttgat gtcccttgcg
     2041 catatcgctt aagtaaggga gccagctctt ttttagtcgt taatacataa tggctttccg
     2101 tttcaaaaat atgcaaagct gagtgcgagt tttgagtgta agattctttt aattctttca
     2161 ctaaacttgt gacttctgct tcgctgagat ttaacagtcc gcttaattct tcattactga
     2221 tcccttcctc gccaacaata aaaagaaggg cttcaagttc acttaatttc gtcatgttcg
     2281 gtatctccta ctgctggata aagcataatt gattcatagt tttctttttg ttgcgcaatg
     2341 atctgccctt ttttcatcaa ttctagcaaa gccataaaag tagtgacaac ttcactttta
     2401 gaaggtgact caaaaaaaga atcgaaagta cagccatgcg catttctttt tatacgctct
     2461 tcgataaata ccattcgttc ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact
     2521 ttttgagaaa atttcttacg ttctaagatt ttctgcatgg cacggatcaa atcgattttt
     2581 ttgacttgtc ctcgcttcaa cggcttttca gcatccgcta acccatcgat gttcataggt
     2641 tcttttgtgt aataaagact tcgttcctct gctttttctg tcagtctttc agctgcatat
     2701 ttatatttac ggtattctaa aagctgagtg accaattctt ctcgaggatc ttcttcccat
     2761 aattcttcat cgtctgtgcc gaattcttgg acaggaagca atgtcttgct tttgattgcc
     2821 atcaatgtag cagccatcac taagtattct cctgcaacag ctagctcgag tgttttcatt
     2881 gtatggatat aattcatata ttgctccgtg atcgtagcga tcggtatatc atatatatcg
     2941 atttccattt gctgaatcag atgaagcagc agatcgagag gaccctcgaa tacatctaat
     3001 ttaatattga tctccgtcaa ctcattcact ctttcttctt tttctttgct agccattttc
     3061 caatgtaagg ctctgtgatt tcagttgcac ggatggagac atccgggtac ttttcctgta
     3121 attcatctaa catttgaaag cccatattct caccgcgata agacggattc aaagaaatgt
     3181 catggatgat cagctgatcg ttcgttgccc agtaaatacc aataatccct tgaatgttgg
     3241 ttgaaccttc tggcgtaaag aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa
     3301 tctccttcaa caaagaagtt ttacctgtca gtttcttatg gaaactcaat agtcccatag
     3361 caattttacg gttttctttc cggtaatgtg ttaacatctt ctcaccttcc tatgactaag
     3421 cccttggaaa atgctgctta taaacatccg tcattctttt cttcgtgata tgtgtataaa
     3481 tctgagtcgt agagatatcc gcgtgaccta acaattcctg tactgtccgc aaatcagctc
     3541 cattttctag cagatgtgtg gcaaagctat gccgcagcgt atgtggtgtt acttctttgt
     3601 aaatcccagc ctctcgtact aactgcttca gatttttcca gattccttgc cgagaaagac
     3661 ctttcccatg attattgaca aatacatgca tttcagatgc gtcctttaca agtaatggac
     3721 gtgcttcgtc taagtagcgt tccagccatt ggatcgcata atcacctaaa ggaatgatcc
     3781 gctctttatc tccttttcca actgtctgta ccagtccaag agaaagatga agatctccca
     3841 atttcaatcc aatcaattca ctgacgcgca tcccagtagc atacatgact tccaagattg
     3901 cacgatctct tatccctaac gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt
     3961 ctgtcagagt caatgtactt ggtaatttct gtgctttctt cggcgtatcg atatgttgca
     4021 tcggatcatg atcagtgata cgctcttgcc gtaaaaactg atggaatctt ctgagacttg
     4081 agatcatccg agtaacagtt gctgtcgctt ttttttcttc atgcaacttc tccagaaaac
     4141 ggatgactaa aaagcgatcg acatcctgcc atgaagcaat tttctgctcc tgtagaaaat
     4201 gcaagtactg ttccaaatca cgttcatagc tttgtcgtgt attggcggac aatccccgtt
     4261 caatattcaa gtagtggaga taatcgttga tttgctcttt cataataatc cccctcatgc
     4321 ctctctatca tatcagatac cgcttcattc tgatagtctt tttccataat ctgagcaaag
     4381 aaaaagccgt ctagacactc agacggactg gtttcttttt tcggctctat aatttatggg
     4441 actgatgaat cagaattgat tcttcagccc catt
//
LOCUS       DAYYOA010000165         4353 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000165 DAYYOA010000000
VERSION     DAYYOA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.168"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACHBKA_002694"
     CDS             complement(<1..1097)
                     /locus_tag="ACHBKA_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9446324.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ACHBKA_002695"
     CDS             complement(1227..2603)
                     /locus_tag="ACHBKA_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9446325.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="ACHBKA_002696"
     CDS             complement(2603..3259)
                     /locus_tag="ACHBKA_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9446326.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..>4353)
                     /locus_tag="ACHBKA_002697"
     CDS             complement(3269..>4353)
                     /locus_tag="ACHBKA_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9446327.1"
                     /translation="NVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQRINREDL
                     SDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLG
                     DEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEIL
                     NNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGI
                     KYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFI
                     SIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILIS
                     IIVLSVVFASSLMLIKKPKEILSKIS"
BASE COUNT         1362 a          790 c          535 g         1666 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taa
//
LOCUS       DAYYOA010000166         4326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000166 DAYYOA010000000
VERSION     DAYYOA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.169"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACHBKA_002698"
     CDS             complement(<1..283)
                     /locus_tag="ACHBKA_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9446328.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="ACHBKA_002699"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="ACHBKA_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="ACHBKA_002700"
     CDS             complement(2361..2723)
                     /locus_tag="ACHBKA_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446329.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3035)
                     /locus_tag="ACHBKA_002701"
     CDS             complement(2727..3035)
                     /locus_tag="ACHBKA_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446330.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3044..3463)
                     /locus_tag="ACHBKA_002702"
     CDS             complement(3044..3463)
                     /locus_tag="ACHBKA_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446331.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="ACHBKA_002703"
     CDS             complement(3456..3689)
                     /locus_tag="ACHBKA_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446332.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>4326)
                     /locus_tag="ACHBKA_002704"
     CDS             complement(3686..>4326)
                     /locus_tag="ACHBKA_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9446333.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1224 a          962 c          620 g         1520 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     4021 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     4081 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     4141 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     4201 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     4261 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     4321 tcggat
//
LOCUS       DAYYOA010000167         4282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000167 DAYYOA010000000
VERSION     DAYYOA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.170"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACHBKA_002705"
     CDS             complement(37..1257)
                     /locus_tag="ACHBKA_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9446334.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACHBKA_002706"
     CDS             complement(1277..1888)
                     /locus_tag="ACHBKA_002706"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9446335.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACHBKA_002707"
     CDS             complement(1931..2863)
                     /locus_tag="ACHBKA_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM9446336.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACHBKA_002708"
     CDS             complement(3284..4144)
                     /locus_tag="ACHBKA_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9446337.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAYYOA010000168         4276 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000168 DAYYOA010000000
VERSION     DAYYOA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.171"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1435
                     /locus_tag="ACHBKA_002709"
     CDS             134..1435
                     /locus_tag="ACHBKA_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9446338.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1462..1722
                     /locus_tag="ACHBKA_002710"
     CDS             1462..1722
                     /locus_tag="ACHBKA_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9446339.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2721..3956)
                     /locus_tag="ACHBKA_002711"
     CDS             complement(2721..3956)
                     /locus_tag="ACHBKA_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9446340.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1247 a          677 c          880 g         1472 t
ORIGIN      
        1 ttcttaaaaa taaatttgat tatttaagag aatatattga aataaaattt cttaattata
       61 taattagtta tgaacattct ttgcgttgtc atgcaatgaa tggttcatat tttattgaaa
      121 aggagtggtt ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg tcccaccaat
      181 ggcaaaaatt ggtacacagc gtcatttatt agcaattcgt aatggggttg tttcaacatt
      241 gtcattgatt ttgattggga cattttttat ggtctttatc aatttaccgt ttcctggatg
      301 gaatgaattt attgcccctt attcggcaac gattgtttta ccctttagga tgacgatggg
      361 attgatggct atttatgcaa cttttgttat gggttcggat ctagctaaat catatggatt
      421 agatagtgtg acaggtggta tattatcttt aggaacgttc tttatgttac aagttcctgt
      481 aaacgtttta actcctgaag aggcacctct tggctgggtc ttacctatga gttctttggg
      541 agcatctggg atgtttgcgg ggatcttatc tatgattttt gcagtggaag tatatcgttt
      601 ctttaaaaat cgaaatatta ctatcaaaat gccagaacaa gtgccacctg ctgttgcccg
      661 tagttttgag gcattgattc caggtgcagt cattttaaca attacttggc tgattcgaag
      721 cgtcgcgggc tttgatgtaa acgctgcctt attatcgtta ttcgagcctt taaccaatat
      781 tttagggaat aatttgcttg gtgttttact accaatgttt ttgatccatt tgttgtggtc
      841 atttggtatc catggcatga gtattgtagg ggctgttgtt cgtccaatgt ggctgatcat
      901 gttggatgag aatgcaaagg cattggcaga tggtacagct gccacccaat taccctatat
      961 cgctcctgaa cagttttatc aatggacggt aacaattggt ggtgcaggag caacgatcgt
     1021 ggtatctgtt ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg ttggtcgttt
     1081 ttctattatt cctggtatct tcaatatcaa tgagcccata atttttggag ctcctatgat
     1141 gttgaatcct tacatgttca tcccgttcaa cttagttcct ttagttttga ctattatcag
     1201 ttattttgca gtcaaattag aaatggtcaa tagatttact gttttgccaa catggacatt
     1261 accagctcca atcggcggtt atctttcagc aggaaatgac tggcgtgttg ttatcttgat
     1321 catgatcaac acattgatag catttattat ctattatcca tttgtaaaag cttatgataa
     1381 aaagatgctg tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg tctaagtttg
     1441 gtttttagaa ggtgggaaac aatgtattgg ctgattttat tttttgtttt tatcttttta
     1501 ttaacggcta gtcaccttat attgaatatg ctagctacat atcatattca aatcaatcga
     1561 tggatttggg cactggcatc atttttgatc gtgattcttc caaagatcat tgtaccgcat
     1621 atgaatgttt tgttttcatg ggggacatat gtactttgtg gaatttttgc tatcaatttt
     1681 atgatcgagc agcatagatg gtttgtcaca tcgaagcttt aaatgttcta aaagtggttg
     1741 taacagaagt ggttagcttc aagaaataag aagaaattta cgaaaattgc ttcttaaatt
     1801 tttgtgaaat gttggcttat ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt
     1861 tgaccaccgt ttattcggaa tatgggactg tgacaaggct tttgtcacag ccctttttta
     1921 cataaagatt attattgttt taactgtttt ggcagaaaga aggaggaaaa aatggaaatt
     1981 ttatcagcaa tggatttaga attagtagat ctactaccaa agtgatcaag tcaaatatag
     2041 atttgggagc ttacaaccat atagtagggg ctgcagtatg gtgtatgaat ggtaatatgt
     2101 atgatgtgag tgtttattca aatcatgaaa cgtgtataga aataattgtg ctgggaatag
     2161 caatcagtca tggtgaacgt gacttttagt gtatcgttgc tgacgacata gaacattctg
     2221 acatgatcta ctcaatatgt ggggattgct ggcagtttat ataggagtat gcatcggatt
     2281 gcgagatcat tgtctcaaca gaagagggag gttgattatc aaagataaag tggtaaatcc
     2341 tacatttgat ttagtaattg ttgaggggac tagcggcaca aatctctaca aagcgatgct
     2401 tctagatcta tatgacacat tttaagaaaa tgtatttgcc taattattat ggtctttctt
     2461 ttgaagaaac gttgagatga tccaaccaga gaaaggtgtc aaagaatttg actaagaata
     2521 aatggcagac gctacctatg ctcatttgga tagcgtcaga tcttttattt taaggatgat
     2581 tttgtgtttt ttagacaccg taatttaagt aactctaact tttcaatcca ctatttactt
     2641 tttttgtatt ttcactgtct gggtctcgaa tagcgaaatt actgatgagt ctctattaaa
     2701 aaagcttttt ctacttgaaa ctatattggg tggttgctca ggaaggtttc gatttcttga
     2761 attgcttgtt gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc ctgctgacga
     2821 atcttgggtg aagaaacttc gcgcagagca atctgcagtt ttctttcagt cactggtttt
     2881 gtgagggcga ttgcgtttcc gtattttaca caatagtcaa tattacagct ttgctcaaat
     2941 tgcatcccca caccgataaa cggtttgccg gattcacagg cagtttggac ggtcccttcc
     3001 ccgccgtgga taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga cagatattct
     3061 gttattaaaa gatcttctga gtcagattga atgtcatcaa gcagatagtc aataggtgca
     3121 ataaccgcta gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa catacgcgga
     3181 ttgcctgaac tacccattgc gaaatagata atacgttttc tttcttgttg ctttcgctgt
     3241 ataaaagtta gcacttcttc aggcaaggaa gtactgagct tagcaaaaat tggaccaacg
     3301 attcgataat tttccggcag ctgttcttct tccaaaaata gtttaggcgt tgtgatgaga
     3361 tttagatcag catccatcat ttgtaaactt gtttttggta aaggaagcat ttctttttgt
     3421 gcagcgtgtt tgaaggcatt ggggatccat cgggtattta ctatacgctt ttttgtccag
     3481 tgccaaaggg gattgcttaa agggccgaag aaacgtaact cgtggggcat cagcgcatgc
     3541 tctgattgaa aaaaaggttc actcaaagca tagggtttaa catatactaa aggaatcttt
     3601 ttattgcggg ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag tcgaggctca
     3661 aacttagcaa tcagactctg ttcactagtg atacgagagg caaccatttc tttggtaaaa
     3721 gggtttttaa gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg ttttttcgta
     3781 aggatcggcg ctagtaaatg atactcaaag ccactttctg tgatcaaagg agcaaaagtt
     3841 ttagaaaagc caaagaatac acagtaataa cggtctttca tagctttggc aacctcaatc
     3901 attcgtgttg tctcagctaa attgaatact gcaggagcaa aaagaattgt cttcatgaaa
     3961 tcctccttat tggaatgagt ctatcccttc gtttcttact agtatactaa aaaacacagc
     4021 gttcgagtag cctatgtata ttttgaatat taaactgtct ctcgctgttt tcacaacttc
     4081 taaatttaaa ttttgtaatc aagttgaaac tccaattttc aaaaaaacac gcaacaattt
     4141 tttgatcgat ttatatgttg tgccagataa atacgagaag ttgtgtcatt aaaaataaac
     4201 atgagaacat aaaaaaataa aataattata ttaattgata ggtgtgctgt cgtcattata
     4261 cacacgtaag aaagaa
//
LOCUS       DAYYOA010000169         4154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000169 DAYYOA010000000
VERSION     DAYYOA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.173"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            395..916
                     /locus_tag="ACHBKA_002712"
     CDS             395..916
                     /locus_tag="ACHBKA_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446341.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLM
                     ELKKYSNTQTKFS"
     gene            1032..1352
                     /locus_tag="ACHBKA_002713"
                     /pseudo
     CDS             1032..1352
                     /locus_tag="ACHBKA_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1530..1889
                     /locus_tag="ACHBKA_002714"
                     /pseudo
     CDS             1530..1889
                     /locus_tag="ACHBKA_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            1953..>4154
                     /locus_tag="ACHBKA_002715"
     CDS             1953..>4154
                     /locus_tag="ACHBKA_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9446342.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVE"
BASE COUNT         1525 a          567 c          763 g         1299 t
ORIGIN      
        1 cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta actttaactg
       61 ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac aatcggaata
      121 gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt gccattccaa
      181 tacgttatgt agtaaatgta atagtttttt tggtagtgag tatgttgagg aatatattag
      241 atttgcagaa gacaataaaa atttccttta taacaataca agtttaaaga atggtaagtc
      301 tgatttaaca cattcaataa agaaaatgaa ttcacttaga gttgcaaaag caattgtagg
      361 aatgtttttt agtttaaatg gggatgaaga ttcaatggat aagccattct tagattcggt
      421 acgagaatat cttagcaatc ctaaaagtac tttatttcct catgaagact acaaaattat
      481 aatgaattat tatcttccaa taggtgctcc acagaattct aaaatcaaaa ttcctaatga
      541 cattgtaaat gatggtgaca ctcacaatgt aataaaaggt tttctgcccc ttgatctatc
      601 aactgggaga aaaattcgat tttcagaaat tcagtatgaa ggaattggtt ttactcttat
      661 agatttaaaa aattcaactt ttgaaataaa agaaggatta gatttaaaaa atctcctttg
      721 tgcagatgat aaaatcaaaa aaatacaatt gtacaaagtc cctatattaa caccaacagt
      781 acttacaatg ttggagatgg aaaataatat taattatatt gactcaggtt cagatttgct
      841 gattaggtgg caggtaataa ctaattttct tatggaatta aaaaaataca gtaatactca
      901 aacgaagttc tcttgaagtt ataaagaata caataaatat aaatttcctt tagactatat
      961 taaaatcagc aatcgaagga aaataaaaga agaaaatggt acgcaaaatc taactattag
     1021 agcaaaaaca ggaagctgct ttggaagata gtcccgaatt gtttccgatg ttaaaagatc
     1081 gtttaaatga attgactgaa aaacgaagac agttacaaat tcgagagaat gaaatcttag
     1141 ggatctttca acaacaaggg gaaccgattc aagtcaaaga tgtccaacag atattgacta
     1201 gtttggattg ctttcttatc cagagtgaga agaagcaagt taaagctttg tatcacacct
     1261 ttattgagaa aatcaccttt gatcctaatc agaagtagat attgccgatc ccgatgagat
     1321 tcacacctac tgtgatttga taattgaatg agtaatatca aatagccgta tccaaaccaa
     1381 aggatatggc tatttttgtg ttagggatat acgtcacgta gcttgttatc aagaataagt
     1441 ttctttattt ttttgctttt tctaaaactg agtatgctaa aataagaggg tatatagcaa
     1501 ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcag aagttattta
     1561 gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat tatttattgg
     1621 ggctgatttt ctataaatat ttatccgacc gtttgttgga acaagtggtt cttttagcag
     1681 atgaatcctt agaggaatat gacacagtaa gcaaacaaac gatgttgtat cgtgaattac
     1741 tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt ttggggtatg
     1801 cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc
     1861 aattgaatga tctaaacaaa gcctttttag caagtcatat ttatggaaaa atactgtcgg
     1921 caaaattcga atagtgtcga ggaggtgctg tgatggaaga attaaaagaa cgcattgtca
     1981 attatgtcat agagaactct gagattaaaa ataaatgggg gaacaataca gcacagaaaa
     2041 tatctgaaaa attaaatgtt agtcgtaata ttgtctctca atatttgaat gaattcgtaa
     2101 aagatggaga atttggaaaa atcaatactc gtccagtaat cttcttttct ttaccggaga
     2161 aagttccaaa tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg tatgatagct
     2221 cttttagggt tggaatagaa agggttatcg gaagtcaagg aagtctgcga aatgtaattg
     2281 ctcaaattaa agcagcaatc tactatccgc caaatggctt acccatttta cttcatggcg
     2341 agactggaac tggaaaaagc tatttagcaa attatgtgta taaggatttg ttggccaata
     2401 attatataga agagaaggca tctttcattt cagttaattg tagtgaatat gccaataatc
     2461 cagaactttt cttgtccaat ttttttgggc atgtaaaagg tgcatataca ggagccgaag
     2521 atggaagaaa gggtttagca gaattagcta ataatggagt cttattttta gatgaaattc
     2581 atgcattgag caatgaatgt caggagaaac tttttcagtt tatggatacg aggaaatttc
     2641 atcggatggg tgataatgaa aactggtaca catcaagttg ctggattata tttgcaacat
     2701 cagaaccacc tgaggataaa ctattatcaa ctttgttgag aagaatacct gtaaagctaa
     2761 cacttccaac attagacagt cgttctcttt atgaaagaaa acagttaatc aagatatttt
     2821 ttaaatcaga agaagaaaga ctagcaaaga aaatactgat atctcaacaa ttattagatt
     2881 ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc attcaattga
     2941 cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt cagatgtatc
     3001 atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag aatgaattaa
     3061 aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc aacgaactta
     3121 tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt tttgagaaat
     3181 atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct ttaacatatc
     3241 ccacctatca agagattcga tttagacagt atgttaagga aatagagaga gattttcaaa
     3301 tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct tggatttatc
     3361 aaatccaatt aacatcggac gataaattat attccttcat caatgatatt aatagttggt
     3421 gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt tatttaacaa
     3481 aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa gaatttcagc
     3541 aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt atagcaagta
     3601 ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca ctaatgactt
     3661 caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc ggatatgaag
     3721 aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat ttggaactag
     3781 acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct attgatgttg
     3841 caaatcagat attattagat ttaccgctta aacagattct tgaacaagta aaagaaaatc
     3901 aaaatgtgga ttatatttat gaatcatcaa atattaagaa ggatatgatt gtctgttctt
     3961 gtgcaagcgg attaggaact tcgtttaaat taaaaaagat acttgaagat agctttccaa
     4021 gggatactga tcttgtcata gaaacgtgtg actattactc tttagtgaat gaaaactatt
     4081 tatcggagct tcgtaaagag aacaatattc tttttattgt tggaacacta aatccacatc
     4141 tagaagatgt agaa
//
LOCUS       DAYYOA010000170         4084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000170 DAYYOA010000000
VERSION     DAYYOA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.176"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(614..2068)
                     /locus_tag="ACHBKA_002716"
     CDS             complement(614..2068)
                     /locus_tag="ACHBKA_002716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446343.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(2069..2368)
                     /locus_tag="ACHBKA_002717"
     CDS             complement(2069..2368)
                     /locus_tag="ACHBKA_002717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446344.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2671..3042)
                     /locus_tag="ACHBKA_002718"
     CDS             complement(2671..3042)
                     /locus_tag="ACHBKA_002718"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9446345.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(3376..3639)
                     /locus_tag="ACHBKA_002719"
     CDS             complement(3376..3639)
                     /locus_tag="ACHBKA_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446346.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         1239 a          846 c          629 g         1370 t
ORIGIN      
        1 aatatcaaca tttgatcaca taatttgaga ttgaatgctg atttacctat gttttacgta
       61 atggtcttta gttatttttg ctcataaaat gcataaaaca cgcgttttta tgtgtgcttg
      121 aaatttcttg gctctataat ttctaagact gataaaaata ttctatcggt tctttttgca
      181 aataaaaaaa catatagaag ctccattaga ttgaatcacc acaacacaat caaaggagca
      241 ttccgtatgc ttgattttca ttctataaaa ctacttttag gaatcaaaga aaatcacaac
      301 ggttcaaact aaaagcaaga atatccgaag taccattgtt tcatcgatca tgagaaaagc
      361 attcacctct gtctgcagat gttcttatat cgctctgtca gtcctattaa taaagtactg
      421 ttttgttttc aaaaaaacta ttacgagtac tttttaagag aatgccttgc aaatattcat
      481 cacacaccgt tatataagtc aaatagtttc tttcagtcgt caagaaaaga ttctcctgtc
      541 cttggcatta tcagttaagt agcaacttaa ttctcggact tgttctactc tagcaaaatt
      601 tattttcatc aacttacgtc caaatgtttt tttgaataat tacttcatcg cgggtctgga
      661 agacttgcct cattcgataa gtcactagct tttggcgtcc ttcaggattt ctagtataca
      721 ccaactgaca tttgggcagc gacgattgat ttaattcgtt tagaaaaagg ttagcgattt
      781 gcttatttcg gccaaagcgt tctggtaccg tcagaactat ctttttccct tcgattaaat
      841 accgcggatt ctccagcggt gctaaaagct ccctcatagc ttcagtaaaa agctgtcctt
      901 ctcgatagcc agcattctca agatagcatc taaattcctg ctgatcagcc gctacgatta
      961 cttttctatc actaacttca ccaaggcgct gcaacgtcgt ttgaacgcat atggccaagc
     1021 tttttttgcg ggcacattcc ttttcccata gcttttgtcg cctacgaatt tggaaatatt
     1081 ccagcaagtt acctttaaat atagctgacc agaagcttag aggctctata ttttttacta
     1141 ctatcggcaa tgaagtttct tgtttttgct ttacctgatc gactttccta acgacgattg
     1201 gcatagatag gtgccagcca tcagctaaag cagtactcca ttgttctttc aaccgacttc
     1261 gttcacaaga aaaatagaac atcgactgag tataagtttc catcgtggtg gtcgtatatt
     1321 ccagtggtaa agcaaagcga tcgccaccac tctcaatttt tggtgaagat tcaaaggtca
     1381 gacctgaaat attgcccaaa cgccgtagca ttcttttcaa atcaggctgt tcagacagag
     1441 gaactcccgg taatccaatc gctaaatgcc aaatgttcgt tactttattt tgtgcaccta
     1501 ggcgtaagcc ccgccctcgt agttgttgtg tttgcacaaa agtggaagct tgattagcca
     1561 agatcaacag attaattgca ggacaatccc agccttctcc tagcaaacta acggttccta
     1621 ccaaaatatt caacttcttt tcggttaata ggcgagtaac tgcttgcatc aatgcttggc
     1681 ggtttttgtc atccaccgtc aaataaaaat aacccgacat ataaggatgt tcattcatac
     1741 ccgtcgctag ttctgccagc tcaacagtta ctagcatttt cggtaacagt aagaactctc
     1801 cacagatggc accgatttgc tgagtgttac ctaatagacg aacaacttca tcaaacaaag
     1861 gaaccacgcc ataagctaag gtaccatcac tgcctgctaa tacctccttg caaatacgat
     1921 ccatcaaaat gaccgctgtc agttcttgct tcagctgaac ttcttctttt aacaaaattt
     1981 gacaaactgc ttgctttttc tcaggaatcg cctgactacc aacagtgtct ggatactctg
     2041 gaaacaaatg cagcgactca tcaaacaatc atccagcttt tttcaattga taaaaaatag
     2101 gcaattccgg ttgatcttca aacatctgct gataaatagt gatcagctct tttttaccta
     2161 aagatggaac tgaagctttt ttaccttgct gcttagaact gcctaatagc tgccaatgtt
     2221 cgggtatcaa ttcatagtta tgatgattca agtaaattaa tgcactagtg aacagctcaa
     2281 aattttcata aatttccttc gaatgctcaa gcggatgctg aacaaattct aaagaaagca
     2341 aataatccac cgtctcttgg ctttccatta acgcttccac cagctcattt tgtttctcta
     2401 aaaaggtaaa ataatgctga gtagtttcct gattagccgg aatcaaatat agaaaatcct
     2461 gatgaggaca tagaacctcc tcctttacca actcgggaat gctaatttct tcgtcaatca
     2521 ggccatttta actcaatata ttgcttccac tccgtctgag agctatcaat cggaggcgtg
     2581 gctgtcaagg aaatcgtcaa cagattcggt tgttttttct tcaagtcaat caacaattca
     2641 ccccagcttt tttttaaatg atgagcttca tcaaaaaact aacagctcca gctgatcacc
     2701 ataagttggc tcctccttca gtcggttgta aatggtctga tacgtttcca ctgtaatatg
     2761 actaggatgt tcaatcgccc cagaaagata gcaactatct gctggattta ctaagaaata
     2821 ttcctcgatt gttgttaacc actgtttctt caatattagg gaaggaacca aaattaaggt
     2881 tttacaatcc aaacgtcgga tcatctcaat tcctaaaaag gtcttgccag cccctggtgg
     2941 agccactaga tggactgaat tgttatttaa ataagtagca aagttagtca atatacgctt
     3001 ctgatagcta cgccactgaa ttttttcatc agatagccac atcccaggtc cccctctcac
     3061 gtattttctc tatcataatc taaatgaata cagcaaaaaa aagatttctt caagaatatg
     3121 tgtttttata gtaatatttt acttactcta aactcaaatg aaaaggtaaa aaaactggta
     3181 gtacaatctt ttaaattctc attgttttgt acgttcttta ctatcaaaaa aacaaaaaaa
     3241 acgcgtttct acgcgtgacc aaaattttta gctctataat ttctaagact gatagaaaat
     3301 cattctgttt ttgaagtctt ttttcataaa tactacttga tttattgtgt atattgtata
     3361 aaagattgtt cataattatt ttttataatt aaaatttggt attgatgacc agcgattccg
     3421 tatataatgc gcaatcaaat taagctttct gtctctggta aatattcctg ttctgttttc
     3481 ttgtactcgt aagatttctt cacttatcgc aaaattcata aaattccaca ctttttcata
     3541 aacaaaatgt acacatttat ctattacttc ataatagtct acttgatatt cttctgtaaa
     3601 catggttgct actgtatctg caccatattt gataaacatt tgggctactc ccttaaattg
     3661 agaaaagccc attagtttat ctactaatac ttctaaaatt tcttgctttc ttctttaaca
     3721 tcgtcccgta aggaatcatg tagtatgctg gaggaagttc attttctaaa taattgtatc
     3781 gcttcaattg ctcaaaggct attctctaca attaattaca ccaattactt tatcttcact
     3841 tatataagat ttctaaacat ttttcgtcaa atgaagagaa aatatttttt taaatgatct
     3901 tgctcaagat ttgtcaacga atctttgaat tcgttttgaa gttctgagga aggtatcatt
     3961 taaaaactca atctgacttt catcatttcg tttactccca ttttgatcac cttctaccaa
     4021 caatttatta aagtgattat actaggtttt tattttttta attcacaatt gtttattcac
     4081 ttca
//
LOCUS       DAYYOA010000171         4053 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000171 DAYYOA010000000
VERSION     DAYYOA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.177"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACHBKA_002720"
     CDS             174..518
                     /locus_tag="ACHBKA_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM9446347.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACHBKA_002721"
     CDS             519..938
                     /locus_tag="ACHBKA_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM9446348.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACHBKA_002722"
     CDS             complement(1030..1380)
                     /locus_tag="ACHBKA_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446349.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2676)
                     /locus_tag="ACHBKA_002723"
                     /pseudo
     CDS             complement(1346..2676)
                     /locus_tag="ACHBKA_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            2932..3498
                     /locus_tag="ACHBKA_002724"
     CDS             2932..3498
                     /locus_tag="ACHBKA_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9446350.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="ACHBKA_002725"
     tRNA            3643..3730
                     /locus_tag="ACHBKA_002725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
BASE COUNT         1477 a          691 c          780 g         1105 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt agg
//
LOCUS       DAYYOA010000172         4010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000172 DAYYOA010000000
VERSION     DAYYOA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.178"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..1627)
                     /locus_tag="ACHBKA_002726"
     CDS             complement(122..1627)
                     /locus_tag="ACHBKA_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9446351.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1624..2310)
                     /locus_tag="ACHBKA_002727"
     CDS             complement(1624..2310)
                     /locus_tag="ACHBKA_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9446352.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2503..3924)
                     /gene="gndA"
                     /locus_tag="ACHBKA_002728"
     CDS             complement(2503..3924)
                     /gene="gndA"
                     /locus_tag="ACHBKA_002728"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM9446353.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1102 a          851 c          688 g         1369 t
ORIGIN      
        1 atgttattct atttaattgt tcgctagttt ttttataatg acatccctga atttctcact
       61 tgcttgtatt ttgtacctta ttctttgttg gatatataaa aaaacaagta aagcacatgc
      121 tttacttgtc ttctgccttt gcgataggaa taaagattct aaaaattgtc ccttgcccta
      181 ctacactttc tgctaagata cgtcctttat agttttcaac cagttgttta gcgatagaca
      241 accccagacc atttccaccc ttcgttctag ctcttgcttt atccactcga taaaaacgat
      301 tgaagatctt attaaggtct tctttgggta ttccttcgcc aaagtcctga atcgcgatct
      361 caaattcatt cagagtggaa gaaatagaga tatggacttc ttttcgtgta gttgaatact
      421 ttactgcgtt atccaatatg ataatgatta attgttcaaa gtgattacga taaatttgca
      481 gggtcacttc ttttgtcaaa tcatcatcta aagtgatcac atagtctgga tataatatct
      541 tgaaattatt ataaacttga taggtgactt cttttccttt agttgtttca ttcgcataat
      601 gaacatctac ttgttctgct cttgataagt ctagcatctc ttgaactaag cttttcatac
      661 ggctgatttc ttgcatgctg gcgcttaatg attcttctaa tatctcagga tcatctttcc
      721 cccaacgatt tagcagacta agatgccctt caataattgc aacaggtgtc cttaactcat
      781 gagatacgtc ttccacgaat tgctcttgtt gttcaatata caaacgcata cggtcaagca
      841 tctcattgaa tatttcagct aaatctgcca attcatcatt tgtgtcgatt tcaggcatgt
      901 ggatatctga ttgcggatct ttacgtatag tatccattgt atctctgagt accttgagtg
      961 gttttaagaa ataagaagat aaaaagaaac ctaaaatact acttaagatc aaagaaacaa
     1021 cttctaaaac aataagagtc aataatagct tatttcgaat atcatagaaa gaagataatt
     1081 catagaagct ttgcgcatag ccgatctttt ctcttgtacc tttagaatat ataggctgga
     1141 tagataaaaa gccagttttt ccttctagtg taacgatcgt tggaacttta cgtgtagtct
     1201 gaatcaggtt tcgactgttt tcatgggttt taaaaatcaa gttctcgtcc aaatcataaa
     1261 cataaagatc cattgatggc tgccctagct cagctataaa ggaatcgatc ttcattaagt
     1321 ttccttcgac cagtgaagta tgatctacga cctcatctcc gtcatttgtc gctttcgtca
     1381 gattacgata cgtatttact aatgtcagtt cttcatccga gttagccaaa cgagaagcta
     1441 cttctgaaat cgtacgctct acattgtttc tttcttttgc cacaatcaga ttgatagatg
     1501 atttataggt aatgacagcg aaaattgtaa acacaacgaa tatgaagaaa gaactcgcaa
     1561 aggcccactt gattgtcaag gagggccctt tcagttctct tttttccgat tttttcggct
     1621 ttatcacgag cgcatcacat aacctgttcc gcgtacagtt tggatatagc tttcctcccc
     1681 gggaacgtca attttgttgc gaagataacg aatatatacg tccactacgt ttgtttcaac
     1741 ttctgtttcg tatccccaca ctttatttag taaaacgtca cgtgctaata ccacgttcac
     1801 gttttccatt aaagtaagca gcaactcgta ttctcttttt gttagttcaa ttacttcatt
     1861 tccgcgacgt acgacacggt tttctttttc gatagtcaaa tcgcgataag taatagtagt
     1921 ttgtttagcc acatttttat cgccttcaat atcgattcga cgcaacaatg cacgaagacg
     1981 tgctaataat tcttcgatag caaatggttt gacgatgtag tcatctgctc cgtggtcaag
     2041 tccagataca cgatcgatca cagaatctct tgcagtcatc ataatgattg gtgtattttt
     2101 gacttggcgt acacgtctgc agacttctaa accgttcaat tctgggagca tcagatccaa
     2161 caatatggca tcccactcat tatttaatgc cgcatctaaa ccagttcggc cgttgtagtg
     2221 gacttctgcg ttataccctt catgcttcaa ctctagctcg acaaatcgtg ccaagttctt
     2281 ttcatcttca atgattagaa tattgctcat ttattatttt atcctttcta ttctactggc
     2341 tttttaaaaa ctctatcatt ataccgataa tatgcttaaa aatctatagt cattttatca
     2401 tggatagcaa ttttaaaaaa gagctgaagt ctactcataa caatcgactt cagctctttc
     2461 gtcctctttt tattttctca tttcctaaca gccgaaagac tattattctt catggtacca
     2521 tgagtaatgg aagattcctt ctttgtcgac acgctcatag gtatgtgcgc caaagtaatc
     2581 gcgttgggct tggattatat ttgcaggcag acgttctgct ctgtatgaat cgaagtaagc
     2641 gatggctgat gagaaagtcg gtacaggtac gccagcttgg acagccaagg caacgacgtc
     2701 tctgactgat tgctgatatt ttttcgtgat ttcaacgaag taatcatcta gcaataaatt
     2761 ttcgatctca ggatttttgt cataggcatc cgtaattttt tgcaagaatc gagctcggat
     2821 gatacatcca gcgcgccaga tttttgcgat ttcaccaaat ggcaagtccc aatcaaattc
     2881 ttttgaagcg acacgtaatt gagcaaatcc ttgggcataa ctcatgattt tactgaaata
     2941 caacgcttct cggatttttt cgaccaattc ttttttgtct cccgtgaact caaaatcgtt
     3001 cgtacgagac aaaatcttgc ttgcttgtac gcgttcttct ttataagcag aaatatagcg
     3061 ggcaaataca gattcagtga tcaatggaag tggcacacct aagtcaagcg cgctttggct
     3121 tgtccattta cctgttcctt tattcccagc tgcatcaagg atcacatcta ctactggttt
     3181 acctgttcct tcatcgtctt tacgtgtcag aatgtcagca gtgatttcta tcaaatagct
     3241 gtctaattcg ccttgattcc attctttaaa gatatcagcc atttcttcta ctgaaaggtt
     3301 taaaatattc ttcattaagt cataagattc agcaatcagt tgcatatctc catactcaat
     3361 accgttgtgt accattttca cataatgtcc ggctccattt gggccaatat atgtgacaca
     3421 tggtgctccg tcttctgctt ttgcagaaat ttgctctaag attggtgcaa ctagcgcata
     3481 ggcttctttt tgtccaccag gcatgattga aggtcctttt aaagctcctt cttcaccacc
     3541 ggaaacgcct gtaccgataa agttgatgcc agagttggct aactcttcat tacgacgcat
     3601 cgtatcttta aagaatgtat ttcctccgtc aatcaaaata tcgcctttat caaggtgagg
     3661 aagtaactct tggattgttg catctgttgc cggtcctgct ttgaccatta gcaagatccg
     3721 acgtggtttc tcgatagatt ctacaaattc ttcgattgta tatgttgctt tgaaattttt
     3781 atctgggtgt tcttctacta cagcggttgt tttagcccct gtacgattga ataaagccac
     3841 tgaatagccg cggctttcta tgttcaaggc tagattttta cccataacgg ccataccgac
     3901 aacgccgatt tgttgttttg acatatgaac tcctcctaca tacgggattt ctccctaatc
     3961 tcttacatta tagcttaaca cggctacatt gcaaactaaa gaacaacata
//
LOCUS       DAYYOA010000173         3995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000173 DAYYOA010000000
VERSION     DAYYOA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.179"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..391)
                     /locus_tag="ACHBKA_002729"
     CDS             complement(242..391)
                     /locus_tag="ACHBKA_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446354.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ACHBKA_002730"
     CDS             853..1065
                     /locus_tag="ACHBKA_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446355.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ACHBKA_002731"
     CDS             complement(1343..3142)
                     /locus_tag="ACHBKA_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM9446356.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ACHBKA_002732"
     CDS             complement(3266..3862)
                     /locus_tag="ACHBKA_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9446357.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1433 a          705 c          687 g         1170 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaa
//
LOCUS       DAYYOA010000174         3925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000174 DAYYOA010000000
VERSION     DAYYOA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.180"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACHBKA_002733"
     CDS             complement(573..1130)
                     /locus_tag="ACHBKA_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446358.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACHBKA_002734"
     CDS             complement(1309..1920)
                     /locus_tag="ACHBKA_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446359.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACHBKA_002735"
     CDS             complement(2033..2290)
                     /locus_tag="ACHBKA_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446360.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACHBKA_002736"
     CDS             complement(2887..3165)
                     /locus_tag="ACHBKA_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446361.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACHBKA_002737"
     CDS             complement(3199..3450)
                     /locus_tag="ACHBKA_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446362.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAYYOA010000175         3876 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000175 DAYYOA010000000
VERSION     DAYYOA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.181"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..915
                     /locus_tag="ACHBKA_002738"
     CDS             25..915
                     /locus_tag="ACHBKA_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM9446363.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(988..1812)
                     /gene="nadE"
                     /locus_tag="ACHBKA_002739"
     CDS             complement(988..1812)
                     /gene="nadE"
                     /locus_tag="ACHBKA_002739"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM9446364.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1824..3296)
                     /locus_tag="ACHBKA_002740"
     CDS             complement(1824..3296)
                     /locus_tag="ACHBKA_002740"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM9446365.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1134 a          795 c          684 g         1263 t
ORIGIN      
        1 aaaacctttt aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta
       61 tatgaagcat tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag
      121 atgaatgtca aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac
      181 gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt
      241 tcgagccttc gtgtcgataa aatcgatatt agttttccag aaaaaatatt caatgctttt
      301 gatacgaaag aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca
      361 tgcggtttag ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg
      421 gatcctcgaa ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag
      481 gcacctaatt ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt
      541 tcttctgaat ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat
      601 ggtgtggagc taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat
      661 acgcctgatt ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt
      721 accaaacact tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc
      781 cctaaagagc aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg
      841 gggggctgta ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa
      901 gtttactaca gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat
      961 gaagggacgg ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg
     1021 caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc
     1081 gggaacttct tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac
     1141 ttcatctgca atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa
     1201 tttttccggt gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa
     1261 taatggcaaa atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc
     1321 tgcagcatga tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt
     1381 aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc
     1441 ttcgcttaat acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac
     1501 atcgggctgg ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata
     1561 aggaagacga acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc
     1621 catcgttagt tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag
     1681 tacaaaagtt ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt
     1741 acgtatttct tcttttggat caattgttgg tttgacacct aattcattga taatttctgt
     1801 ctgcaggctc atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc
     1861 attcgttcca tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat
     1921 ttttgcggat tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta
     1981 tattctttga tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata
     2041 tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact
     2101 ggatggaaca tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag
     2161 tctccttcag atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga
     2221 gtagttactt tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca
     2281 tcttcgatcg atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc
     2341 aattttgtcc caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc
     2401 gttgcttcat ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct
     2461 aattgttccc gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct
     2521 tggaagttga ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat
     2581 ttcaacgtat cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc
     2641 ataaatgctt cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta
     2701 gctggtatcc caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata
     2761 tatgccgctc tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat
     2821 tccaatagtg gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac
     2881 gtttggaaat tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc
     2941 ccttccactt ggatcaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga
     3001 actgttgctt taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct
     3061 aatccacgca agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact
     3121 aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa
     3181 cattcaaaaa cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg
     3241 atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg
     3301 tattactcct cttggcaagt tttcatttcc tacccattat aacaaaaaaa ttaataggaa
     3361 aagaaatctt ccattttttc tgaaaaagca catgttttac ggatatttct tgctaaaaat
     3421 gaactccatc tgctaaaact atcctttctt attggtattt aacctaaacg agaaagaata
     3481 cttattttat tcaatgtatc cgagacacga agaaattagc gttttctatt tttcttactt
     3541 gataaaataa gtagattttc ctactacaat cgtagccgtt aagataccga tgattgaaca
     3601 aatataacca aatgtctcta caacataatc aatggcaaaa gtttttgaag caataatacg
     3661 tctttccttt tttgcttctt atccccaaag aaacggatat aacttattat gtattgaatt
     3721 tttttggaaa ggcgaattat gacaagatat accttgcgtt gaaaaaactc gcggaaaaaa
     3781 tatcacacgt ttttaaaaag atacaaagta agaaagcagc tgtcaataga atggaaacaa
     3841 aaatggcgag acatcaatca gtctcgcctt gttttt
//
LOCUS       DAYYOA010000176         3875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000176 DAYYOA010000000
VERSION     DAYYOA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.182"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACHBKA_002741"
     CDS             158..2257
                     /locus_tag="ACHBKA_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM9446366.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACHBKA_002742"
     CDS             2453..3316
                     /locus_tag="ACHBKA_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM9446367.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3875
                     /locus_tag="ACHBKA_002743"
     CDS             3421..>3875
                     /locus_tag="ACHBKA_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9446368.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1543 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DAYYOA010000177         3759 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000177 DAYYOA010000000
VERSION     DAYYOA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.183"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..296)
                     /locus_tag="ACHBKA_002744"
     CDS             complement(<1..296)
                     /locus_tag="ACHBKA_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446369.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILL"
     gene            complement(594..1568)
                     /gene="sgrA"
                     /locus_tag="ACHBKA_002745"
     CDS             complement(594..1568)
                     /gene="sgrA"
                     /locus_tag="ACHBKA_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM9446370.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1764..2603)
                     /locus_tag="ACHBKA_002746"
     CDS             complement(1764..2603)
                     /locus_tag="ACHBKA_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9446371.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2788..3675
                     /locus_tag="ACHBKA_002747"
     CDS             2788..3675
                     /locus_tag="ACHBKA_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM9446372.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         1263 a          762 c          555 g         1179 t
ORIGIN      
        1 agtaaaatat aaccaatcgt tccctctaaa agtatagaat aaactttact cagtaaaaac
       61 tgagaagaaa actcgacttt tatcatcttg ttttctgaaa gaaaagataa tttatattct
      121 ggataccttt cgcaaaacga ttctgcttgt tgaatcaccg aattcatctt agccagggtg
      181 tagtttagat ctaaagcgat atccttcacc acatataatt tctcgtctct ttcataacaa
      241 tagttcaaaa attgatatac ttgcctttca ttgatatcaa taaattcttc aaacaaagta
      301 catactcctt tttatctatt atttagactt attccgacag ctacatgaat aacgtacaaa
      361 tagattcgtt actgattttt cagagatact accatgcgca gtgtgcattg tcaattattc
      421 ttgctcttat tttccatttg tgttcctgtt atttcctgtt tctcaaatat gttagtaaac
      481 aaaaatagat ctggcaaagc tacaacttcg ccaggcaata cagcactctc gaatgaaata
      541 ttcactcaaa aatctatcgt tttattcaat tttagatctg ttgcttttat tctttatttc
      601 actcgatgga agagagaacg aaaatagctt gctcctacaa tcaaaatgag taacacaccc
      661 atccatgtaa ttagcttgtt caaggttcta ctaccagttt ctggtaaatc gctttttcta
      721 ttatctgttt ctttcgcttt ttcttgatga tcaattccac gagaaacaaa atcaggctgt
      781 tttagcacac tttttaggtt gctccctgca tttctctttt gttccttagt tggtatgata
      841 ggtaccaaat caagttcatt atttactttt cctgtgctgc tttcgctgct cgtgcttcct
      901 gtgctactct cactactcat gcttcctgtg ctactttcac tactcgtact ctccgtactg
      961 ctttcgctgc tcgtgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg
     1021 ctgctcgtgt tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc
     1081 gtgttttctg tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact
     1141 gtccaactgc ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc
     1201 aaatcaactt tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt
     1261 gtatactcaa taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt
     1321 tctgaacttc cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc
     1381 acgcttttgt ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc
     1441 cgatcagttt tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg
     1501 attccaatca ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt
     1561 ttcttcattt ctatcactct ccatataatt accttctatt tgataaagga attatataaa
     1621 atgaaaatga tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt
     1681 cgcttgaatt tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat
     1741 atcagccttg ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt
     1801 gttttatatg catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat
     1861 atagatgctt cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga
     1921 gtggagaaaa aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt
     1981 gtccaattcc aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata
     2041 agtgcgctga aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta
     2101 ggattcaatc cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga
     2161 ccaaatctaa gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc
     2221 actgcaataa ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct
     2281 aaccgatacg cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca
     2341 acaacgagac ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct
     2401 tctattcttt ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa
     2461 tgtccgaccg taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca
     2521 agtgcattat tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca
     2581 atcaaccgaa taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt
     2641 ttctttcttg tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt
     2701 acgaactaag aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc
     2761 tgttagactg tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt
     2821 atgtgccttt cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg
     2881 attgcaacgt ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt
     2941 aaatcaaata atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta
     3001 tacggaaaag agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa
     3061 ctcggtccaa actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag
     3121 aaactcttta aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca
     3181 gataaagatc tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt
     3241 ctattttcga ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggggaaaat
     3301 ttctccaagc ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa
     3361 atgaattttg attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa
     3421 ctaaaaaacg gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa
     3481 agctattatc tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac
     3541 aaatctgagc tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat
     3601 aaaacaatcc gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa
     3661 aatatattca aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat
     3721 ttttggaaat tccttcttat ttttgaggca aaccacttt
//
LOCUS       DAYYOA010000178         3747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000178 DAYYOA010000000
VERSION     DAYYOA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.184"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="ACHBKA_002748"
     CDS             complement(<1..2303)
                     /locus_tag="ACHBKA_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9446373.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ACHBKA_002749"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ACHBKA_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1079 a          813 c          576 g         1279 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gaatccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacac ttttataaaa cgacgaaaaa taaatcacta ccagtcaact
     3541 tctataatat taccaaataa caaactaact atccatcaat aacgtgtttt attttttaaa
     3601 atttctataa aaaaacaatc attcttaaaa agaatatata aaaataacaa taacctttgt
     3661 gatttttctt gtttgaagaa tgttagaaga aaaatctcta aatacttata attattttat
     3721 aagcaaaaaa atgctatcgg tagtatc
//
LOCUS       DAYYOA010000179         3713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000179 DAYYOA010000000
VERSION     DAYYOA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.185"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHBKA_002750"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHBKA_002750"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM9446374.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACHBKA_002751"
     CDS             complement(2261..2512)
                     /locus_tag="ACHBKA_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM9446375.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACHBKA_002752"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACHBKA_002752"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM9446376.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACHBKA_002753"
     CDS             3462..3680
                     /locus_tag="ACHBKA_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446377.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DAYYOA010000180         3418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000180 DAYYOA010000000
VERSION     DAYYOA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.186"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..191
                     /locus_tag="ACHBKA_002754"
     CDS             <1..191
                     /locus_tag="ACHBKA_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446378.1"
                     /translation="VIGKYLGKKSFSELRDEEIVPTPMVSKIRKNKAEGNYYLGTIAK
                     IIDYLENKEQKKGTKKGR"
     gene            310..1368
                     /locus_tag="ACHBKA_002755"
     CDS             310..1368
                     /locus_tag="ACHBKA_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFM9446379.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            1410..3359
                     /locus_tag="ACHBKA_002756"
     CDS             1410..3359
                     /locus_tag="ACHBKA_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9446380.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
BASE COUNT         1258 a          513 c          697 g          950 t
ORIGIN      
        1 ctgttattgg aaaatatcta ggtaaaaaat ctttcagtga attgagagat gaagagattg
       61 tacctactcc aatggttagc aaaatccgaa aaaacaaagc tgaggggaat tactatcttg
      121 gtacaatagc aaaaatcatt gattatttgg aaaataaaga gcaaaagaaa ggaacgaaga
      181 aaggaagata atttttgtgt taaatattaa tggggaagaa tataccgaaa tgattcaaaa
      241 tcccgaacct agaattaccg aaaaaacgtc tgattgtttg ctcttttatg aaagggggca
      301 gacaaactga tggaaaaaaa cgtcaattca acatctacca aagatcaata cagtattatt
      361 ggagggttgc ttggtgataa ggataagaga acacgtcacg gattacgcat tatgcttgca
      421 acttttctag gagtgctttt tttattaaca ggcttgggta gctacattta tcaattagga
      481 aactataatt attatctcgt gaacaataca acacctatag ggtcttcatt ggagtttgct
      541 cgatcaggag cagaaatcac tatttcagat gtgtggacag atcaaaatag agatgtaaca
      601 gtagtaaaac ttggttactc ccaaaacgct agaaagttgt tatctacgca aggaaaaaac
      661 tataacttat atatggcaac aaaagaaaaa gatcgtccta agaagcttga aatgagctat
      721 gggatgttaa gtactgatgg ggatgctttc ttattcctaa aaggtaaatt agaggaaaga
      781 gcttatcaag tttttattgc aaaccaagta tcactaattt ctggtgatga tacagctgac
      841 gatgatacca aagtcaatgt gaatgaagca gattcagtta caaaagcact ttctcaatat
      901 tctatgaatg atgttaataa tgaaggagta atttcttttg ggaaaaaagc aaaacctgtt
      961 aatactcctg ataatatcaa tttcagaata aatccatatt cagattcaac taatgtctat
     1021 caaggaagct ttcttaccaa agatggcgat attgattatt caaaagttgt agcagttaca
     1081 agtaagaata atgttgtaaa taagctagaa gatcagttaa atgcttcaaa tactaacatc
     1141 aagaaaattg aagccacaaa agctgagtat ctggaacgtt tacaacaaaa tcctgacgat
     1201 aaagattcgc aagaaagtat tcgtcaacta gacagtcgaa tggagaccga aattaaagca
     1261 atcaatgata cacaaaaatt gattgatcaa tacaaaaatg ctgactttaa caaagattct
     1321 tttggagaca tgaagacgac atttaagtac ctaactttga ctgactagaa gtaaattatt
     1381 cttattttag aggaagtgaa cacaaacaga tggaaaatag taccgagttt tttaagaata
     1441 ttcaaaccga atttaatcat gctctcacac caatgaaacc accaaaacat aagttagtgg
     1501 gacgtgatga cgaactccat gagttgtcac tgatcttgaa tagaccggaa acaccgcaag
     1561 ctatcttgtt aggtgaagcc ggaacaggta aaacagcact tgcaagtgaa tgggctgaaa
     1621 gacagcgaca agatggaaag aaagtacaag tatataaatt atcaatcgga caattgcgtg
     1681 ttgggggaga tgatgtcctc caaacacgtt tagaaacatt aattccaaga ttgaaagaat
     1741 atgagcgaat tttaaaagct gaggacaaag ataatgaatt tgtcctcttc attgacgagt
     1801 ttcataaagt tgttagctct tttgggagtg gcaataaaat tgggggtgat tcgctaaaag
     1861 aaccgttagc aaatgaatca cttcaaatga ttaccgctac tacaagaaaa gaatatgaaa
     1921 actatatcaa aagagatgat gccttaggga gacggctaca accgttagaa ttgaatgaac
     1981 taaaaagcga tgttgtcgtc gatattttga gacaatggct aaatacatcc tctgttaaag
     2041 atgatcctca ggcttctttt agattatcgc aaaggattcc tgatgaatta cttcaagaaa
     2101 tcgtacaagc aaacaaactt tatcgtgagg atttgtccga accggctaaa tcaattgctg
     2161 taatggagga tttgattgca aaagatcgag tagacggaaa tgcttataat cgagaaagtc
     2221 tattgcaaat atttggtaag tataatattc agctagattc tgattttgat atggaaaaac
     2281 ttcaagagga attaaagtcc ataaaaggac aaccgttagc agtttatact ataacaatgt
     2341 tgtttaaacg tatgaagctt ggatttgatc gttttaaagg tcgaaagcaa ccaaaagcaa
     2401 ctgctctgtt tacaggatca acaggggtcg gtaaaactgc aatggctaaa gccgtggcaa
     2461 gaggacttta tggcgatgaa aataaagttc tcttagttaa tatgccggac tacaaaacca
     2521 aagatgatgc aatgcgtttt agacgactta taggtactca tgtaaggcat gatccttctg
     2581 cgattattgt gtgtgatgaa tatgagaaag ctggtgtaga aacactcgat gcctttttat
     2641 caattttaga tgaaggtagg ataagtttta ctgaaagagg atgggatgat cgagaagttc
     2701 acgacaccgt ttcattgtca aatacaattt tatttgcgac aacaaatgca ggtgcggaga
     2761 tgttcgattc gttaaaccga tatagctcac tatctcgtgt tacaaacaag gaaaattcta
     2821 atgaatacag cactgaacta aaaagagaat accgaaaagt cgcatcaggg atcaaacaag
     2881 ccttgattgg tgaaggtcta aagcctgaag ttattaaccg atttggacga atagttccat
     2941 tttgggcttt atccgaacaa actcttctgg aaattgctta taatgaaatg ttgaagagtg
     3001 ctgaattagt acgaaaacat ttaaatatag atttacgtct tccacctagg aaagaaagag
     3061 tatatggtca aaatttttat gcagacgatc tctccatgca cttagttttt gacatggctt
     3121 cacaagagga ttctaaatca gggggtgcaa ggaatatctt gcatttagtt gaatctgagt
     3181 ttgaagaagc gattactgaa gcaataatta cttatccaaa cgcaaaaata ttcgatgtta
     3241 agttgagtga agccggagcg tttgaaggaa aaggtgattc taatgctgaa attattgcaa
     3301 agccttccgt tgctgaaagt aaagaaaaca agattgtatt ttcgattgga ggggcgtagc
     3361 tatgaaattc aagaaatatt tactacaaaa ccttagaata aaacgatggg tcgtaaga
//
LOCUS       DAYYOA010000181         3274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000181 DAYYOA010000000
VERSION     DAYYOA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.187"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            590..1006
                     /locus_tag="ACHBKA_002757"
     CDS             590..1006
                     /locus_tag="ACHBKA_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM9446381.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            1020..1586
                     /locus_tag="ACHBKA_002758"
     CDS             1020..1586
                     /locus_tag="ACHBKA_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM9446382.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1609..2595
                     /locus_tag="ACHBKA_002759"
     CDS             1609..2595
                     /locus_tag="ACHBKA_002759"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9446383.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2750..>3274)
                     /gene="scm"
                     /locus_tag="ACHBKA_002760"
     CDS             complement(2750..>3274)
                     /gene="scm"
                     /locus_tag="ACHBKA_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9446384.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT          980 a          612 c          698 g          984 t
ORIGIN      
        1 ttttgttgct ttcgcattga tgaacggttc cggacgtgcg attgggttgg atcgctgggt
       61 aattccatgg ctacaacgta cactaggtaa ttggtggtac ggtagaccaa gttcccagta
      121 cggcaagaaa taaatagata aagaacgtta tttgaatgca agatttcaat agaaaggggc
      181 tgagacaaac cgtcacagcc tcctttttat gtaaaattaa aggaataaga tgcccgagat
      241 aagcctccat gagcaagaca caggtgtcac aacctctgtt acaacctctt gttataaacg
      301 gtgttccaga agtagcttct tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta
      361 attttcgtaa attccttctt atttcttgaa gttgcccact tctgtcacaa cctcttgtta
      421 taaacggtgt tccacctgct gcttctcggt aataagccca aagaattaga aaacatggaa
      481 agctgttttc tattcttcgt tcttattact caaagcaaga cacaggtgtc acaacctctt
      541 gtttttatgc taaaattggg tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa
      601 agaatttgct gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt tgcttttgac
      661 tgttctttct tttttaccag tcatgatttt tggtcttcag caaaacaccg caacagcaga
      721 aaaagaagcg gtactgcgtg tggatggcac agaaatcaaa acattccctt tgatggctgg
      781 agaagagagc aacacatata cttacaaaga tgaacacgga gattataact tgatcgagat
      841 agatggcgat cgagtacgga taaaagaagc agattgcgat gaccagatct gcgtacgtcg
      901 aggctgggca acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat tagtgatcga
      961 agtccgttca acagatggag gtgacgaaga cagtctgatt tattagacct tcacacacca
     1021 tgagtaaatt acaaaaaatg atttatattt cgctacttgt tgcacaagga gtgatcattg
     1081 gtttattgga aaatatgatc ccttatcctt tttcttttgc gcctggtgca aaactaggct
     1141 tagccaatct aatcacgatc gttgccattt ttaccatgaa aaaacaagac agcttcttac
     1201 tactttggct acgactattc ctgactactt tgctaggcgg aacgatttcc acattccttt
     1261 atagtatgag tggtgctttg cttagttatt ttggaatgtt attggtcaag caattaggac
     1321 caaaacgtgt cagtatcatc ggaattagtg cggccggcgg tttcatgcat aatgtcggac
     1381 agcttttgat ggcttctttc attgcacaat catggacagt catgttgtac ttgccagtcc
     1441 tttcagtttt agggatttta tcagggattg caatcgggat cgcagcgaat tatcttttac
     1501 gacatgtgca gacacttcaa cgattccaaa aagattatga gcagggaacc aaacaaaaat
     1561 ggagcccaat gtttaccaaa aaataatcgt cacacaagga gtttttttat gaagctacat
     1621 ccaatgtggg aaaactatcc tgctttaagt aaagaattga cagaaacact aaaacggatg
     1681 gaagaaactg tccgcctgaa aaacaaacct gtcgaacaag ccatcaaaga aacgatccat
     1741 gccggcggaa agctgttgcg tcctgcctat cagctattgt tctcacaatt tggaccagag
     1801 caagatcgag acaaagccat cgctttggca gcttctatcg aaatgctcca tacggccaca
     1861 ctgatccatg atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc taatatccgt
     1921 agtcagtttg gtaatgtcgt ttctgtctat gcaggagact atttatttgt ctgctgtttt
     1981 aaactattgt ctgattactc cacttccttg aaaagtctcc aattgaattc tcgcagtatg
     2041 gaaaaagtat tgaatggcga attagggcag atggatgatc gctataatta tgaactttct
     2101 gtaaaagaat atttggaaaa tatttcaggt aagacagctg aactgttcca attaagttgt
     2161 tctgttggcg cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc aggagatatc
     2221 ggactttcta tcgggatggc ctttcagatt atcgatgata ttttagatta tacaaaagag
     2281 agccaggaaa tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta ttctttaccg
     2341 ttgatctact ctttgcaaaa aaacaaacca aagctactac cttatttaga gaagaaagcg
     2401 gcaatgacag aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg
     2461 gaagaagcta ggaagttagc ggcctcttac acagaaaagg ctttgaagga aataaagaaa
     2521 ttacctgcca cctctcttcg aacaaaagag aatctctttt cccttaccca actgattttg
     2581 gatcgcaaag actgaatagg gaaaggttcg atacgtaaat aaatttctca gaaggaaatc
     2641 tacgtgtttt ttgacaaatt gaattgtagt tcttgtattt atgataaaaa gcaaaaataa
     2701 agagcaacgt ccgttagcga cgttgctctt ttttatcttg aatatttttt taccttttcg
     2761 atttcttata actcataaaa gtgattctta aaccgattac aagtaatata acgcctgcga
     2821 gtgttatcca attattcgat tgacttccag ttttaggaag tgctcttttt gatttgtttc
     2881 cattgtcatt gcttgtttta ttagttaagt taaagttcgt cctgttatcc gaacttgttt
     2941 ttgagaagga gacacttgca tgacgtgtat cgcttgtggt agacgacgtg tctgtactat
     3001 cgcttgaagt tgatgaatct gagctatcac ttgtggtaga ggatgtatct gtactgtcac
     3061 ttgaagctga cgtgtccgtg ctatcacttg tagtggtaga tgaatctgag ctatcacttg
     3121 tggtagaggt agtatctgta ctgtcacttg aagctgacgt gtccgtgcta tcacttgttg
     3181 tggtagatgt atccgtactg tcgcttgaag ctgacgtgtc cgtgctatca cttgtggtag
     3241 aggtagtatc tgtactgtca cttgaagctg atgt
//
LOCUS       DAYYOA010000182         3221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000182 DAYYOA010000000
VERSION     DAYYOA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.188"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHBKA_002761"
     CDS             301..585
                     /locus_tag="ACHBKA_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446385.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHBKA_002762"
     CDS             588..1004
                     /locus_tag="ACHBKA_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446386.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHBKA_002763"
     CDS             complement(1141..1860)
                     /locus_tag="ACHBKA_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9446387.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACHBKA_002764"
     CDS             2544..2630
                     /locus_tag="ACHBKA_002764"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9446388.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACHBKA_002765"
     CDS             complement(2932..>3221)
                     /locus_tag="ACHBKA_002765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446389.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAYYOA010000183         3174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000183 DAYYOA010000000
VERSION     DAYYOA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.189"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHBKA_002766"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHBKA_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHBKA_002767"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHBKA_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="ACHBKA_002768"
     CDS             2092..3132
                     /locus_tag="ACHBKA_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9446390.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT         1172 a          446 c          462 g         1094 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat tgaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa catt
//
LOCUS       DAYYOA010000184         3128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000184 DAYYOA010000000
VERSION     DAYYOA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.190"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACHBKA_002769"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACHBKA_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ACHBKA_002770"
     CDS             1360..1992
                     /locus_tag="ACHBKA_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9446391.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2200..2424
                     /locus_tag="ACHBKA_002771"
     CDS             2200..2424
                     /locus_tag="ACHBKA_002771"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFM9446392.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2526..2759
                     /gene="relB"
                     /locus_tag="ACHBKA_002772"
     CDS             2526..2759
                     /gene="relB"
                     /locus_tag="ACHBKA_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9446393.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ACHBKA_002773"
     CDS             2749..3018
                     /locus_tag="ACHBKA_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9446394.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAYYOA010000185         3052 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000185 DAYYOA010000000
VERSION     DAYYOA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.191"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..352)
                     /locus_tag="ACHBKA_002774"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="ACHBKA_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="ACHBKA_002775"
     CDS             complement(612..1160)
                     /locus_tag="ACHBKA_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446395.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="ACHBKA_002776"
     CDS             complement(1161..2018)
                     /locus_tag="ACHBKA_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9446396.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="ACHBKA_002777"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="ACHBKA_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="ACHBKA_002778"
     CDS             complement(2580..2909)
                     /locus_tag="ACHBKA_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9446397.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2986..>3052)
                     /locus_tag="ACHBKA_002779"
     CDS             complement(2986..>3052)
                     /locus_tag="ACHBKA_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9446398.1"
                     /translation="KVDEDEHVVEIYSARTHYESQ"
BASE COUNT          892 a          620 c          384 g         1156 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct ta
//
LOCUS       DAYYOA010000186         3010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000186 DAYYOA010000000
VERSION     DAYYOA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.192"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHBKA_002780"
     CDS             <1..1412
                     /locus_tag="ACHBKA_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM9446399.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="ACHBKA_002781"
     CDS             1546..2889
                     /locus_tag="ACHBKA_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9446400.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1197 a          406 c          578 g          829 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat
//
LOCUS       DAYYOA010000187         2980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000187 DAYYOA010000000
VERSION     DAYYOA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.193"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(197..811)
                     /locus_tag="ACHBKA_002782"
     CDS             complement(197..811)
                     /locus_tag="ACHBKA_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9446401.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1417..1794)
                     /locus_tag="ACHBKA_002783"
     CDS             complement(1417..1794)
                     /locus_tag="ACHBKA_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446402.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2094..2975)
                     /gene="galU"
                     /locus_tag="ACHBKA_002784"
     CDS             complement(2094..2975)
                     /gene="galU"
                     /locus_tag="ACHBKA_002784"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9446403.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          905 a          624 c          467 g          984 t
ORIGIN      
        1 aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga
       61 tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat
      121 cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt
      181 gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat
      241 acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat
      301 ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat
      361 cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat
      421 caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga
      481 tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag
      541 aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg
      601 gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta
      661 caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg
      721 cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt
      781 ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca
      841 tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa
      901 cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc
      961 ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt
     1021 ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg
     1081 atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt
     1141 cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc
     1201 caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca
     1261 taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat
     1321 ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc
     1381 attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct
     1441 actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat
     1501 ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa
     1561 agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc
     1621 aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa
     1681 catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat
     1741 cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa
     1801 cacacacctt tttttataat taaaaaattc gcctttttat tctactatga atcttctggc
     1861 ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac
     1921 cattagcata atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca
     1981 cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc
     2041 tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt
     2101 tctgttctta gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc
     2161 gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg
     2221 tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt
     2281 gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat
     2341 attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt
     2401 tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga
     2461 tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa
     2521 gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc
     2581 aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc
     2641 acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc
     2701 gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat
     2761 tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag
     2821 atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc
     2881 acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc
     2941 ccagcggcag gaatgactgc ttttttgacc ttcacttgct
//
LOCUS       DAYYOA010000188         2877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000188 DAYYOA010000000
VERSION     DAYYOA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.194"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            799..1053
                     /locus_tag="ACHBKA_002785"
                     /pseudo
     CDS             799..1053
                     /locus_tag="ACHBKA_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1123..1635
                     /locus_tag="ACHBKA_002786"
     CDS             1123..1635
                     /locus_tag="ACHBKA_002786"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9446404.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1632..2669
                     /locus_tag="ACHBKA_002787"
     CDS             1632..2669
                     /locus_tag="ACHBKA_002787"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9446405.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          508 c          573 g          755 t
ORIGIN      
        1 attgatgacc acgaaagtgc atggcgatat ttgatgttga tactttatgc agtaccctat
       61 gtattcttcg ctatcattct gtggctcata gcattgtttt ttagagtatt taatgacctt
      121 taggtagatt tatatataaa aagggctaaa tacgcaaaat acccccttaa aatgcaaaat
      181 aaatgcaaat aagggggtaa ctccttatga aaaaacaact aatagtatta taacacgttt
      241 ttatgccgag aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt
      301 tggttggact tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc
      361 ctgtaggttc cgaccaataa ggaaattgga ataaagcaac gaaaggagtt gagtaaaatg
      421 agtgagaaaa cgaagaaaat cattcgagta tcattagctt tttcacaagt agtagtgcaa
      481 tcattgatag tttattatac ttaccagcaa tataaattat ctaaagagaa ttaagttttg
      541 attatgtaat aagttttggt actaaaaaag acttgatcta attagaccaa gtcttttgtt
      601 agtgttatat taataacaaa ataaaaataa gaagtcgctc actccccgac caaagtttgt
      661 gagcaaaata aacagtcgat gtttgtttct taattgtata tcttgatatt aaacgattta
      721 agattattct tcaagatata tattcgcaaa tgtaattgga aaagcgactt cttaaacaaa
      781 attaaggagt cgctttttat ggcaaaaata acgaaaaatg agcaagaaaa tttggtggaa
      841 aattacacaa aaaaaccggc gtactctaat agccggttag acgcacattt cgtgtgcact
      901 tctaatccga aattgagctt tgacgctatg acgatagtcg gaaaccttaa taaaaacagc
      961 gctaaaaagc tatccgattt tatgagttta gatccacaaa ttagactttg ggatatactt
     1021 caaacaaaat ttaaagctaa agcactacaa gaccccctat gttaggctag ttgtcgtata
     1081 ggataataag ttataataaa gactatacgg aggatgatta ccatgacaaa taaaaacaat
     1141 gaacgttact ctaccgaact gaaagcgaca gccatcaaga aaatgatgcc acccgaaaat
     1201 agggggattc gccaattaag tcaaactttg ggcattccag aagcgactct ttattcttgg
     1261 cgtaaaaaag ctagaattca agggaatact acacccggaa cgaaaaaaaa tgtcgaaaaa
     1321 tggtcaagtg aagataaatt tttaattgtg atggaatcat tctcaatgaa ccaagtagaa
     1381 cttgcggaat attgccgaaa gaagggctta tttaaagaac aaattgaggc ttggaagaaa
     1441 acgtgcttat ctgcgaatga acaggaagaa aatcgaacga gagaacttgc aactgaacta
     1501 aaagaagaga aaaaacaagc gcgtcaatta gagaaagaat tacgtcataa ggaaaaagca
     1561 ttagcggaag ccgctgctct cttattacta agaaaaaaag cacacgcgat ttggggggac
     1621 caagaggaag aatgatttca tcttcagatc gcaaacaagc tgttgaactt attcaagaag
     1681 ccaatcgcaa tggggcccgt ctcacctatg cctgtaatga gctgaatatt agcgttcgaa
     1741 cctacgaacg atggacgcgt gaaggtacga tagcgcatga tcaacgccct ttggcgaaac
     1801 gtcctgtacc caaaaataaa ctgacggatc aagaacgcga aaaaataata gaaaccgttt
     1861 caaaaaaaga atttatgaat ttgccaccta gccaaattgt accaaaactt gcggattgct
     1921 cgatttatat tgcatcagag tctagcttct atcgtgtatt gcgagaaaaa aatatgcaac
     1981 atcaccgtgg ccgaagtcaa gtttcacaaa aacgcatacc gccaagtcac cttgcgacga
     2041 agccaaatga agtttggact tgggatatta cttggctaaa aggtccaata aaaggactct
     2101 tttaccggtt atatctcatc attgatttat ttagccgtaa aatggtaggc tgggagattt
     2161 gggaatcaga agaagctcgt catgccgaaa aacttattaa acagacggtg cttaaggaac
     2221 gtatccaagg acagccgttg gttttacact ccgataatgg cagccctatg aaagccgcga
     2281 cttttcaagt cttactcgaa aaattaggaa ttcaaagttc ttattcaaga ccacgagtga
     2341 gtaatgacaa tccttattcg gaagcggctt tccgaacgtt aaaatatcga ccagaatatc
     2401 ccaataacgg ctttaaaaca atagatgatg ctcgaaaatg gacgaaagag tttgtcgatt
     2461 ggtataacga ttctcatttg catagtggga ttcaatttgt gtctcctgca gcgtgtcatg
     2521 aaggacgaca tctcgctatt ttagagaaac gaaagcaggt ttacgaggac gctaaaaagc
     2581 gccatcctga acgctggtca aaaggtgttc gaaattggac tccacatcaa tcggtggcgt
     2641 tgaatccttt aaaagaaaca gaaagttaac aggagcataa tctttcaaga ttatcaaata
     2701 accctcgtca cccaataggg gaaaaagtta ttcttcccct attggataac gaggccaacc
     2761 gaccataggg aggttctgct ttttgatagg tggaaaataa aaatacgaca actatattga
     2821 caaacaccga ccatcgtcca gtaaagagac gcaataaatt ctatcgaagt ttacgca
//
LOCUS       DAYYOA010000189         2875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000189 DAYYOA010000000
VERSION     DAYYOA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.195"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..2130
                     /locus_tag="ACHBKA_002788"
     CDS             70..2130
                     /locus_tag="ACHBKA_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM9446406.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2213..2782
                     /locus_tag="ACHBKA_002789"
     CDS             2213..2782
                     /locus_tag="ACHBKA_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9446407.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1034 a          518 c          558 g          765 t
ORIGIN      
        1 atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac
       61 taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta
      121 gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg
      181 atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc
      241 aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact
      301 ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt
      361 tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa
      421 accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat
      481 cagttaaaac agatcactga tattttgaat catgctttga cagatataaa ggacggtgga
      541 agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc
      601 gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca
      661 gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg
      721 actaatacag gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca
      781 atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat
      841 cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa
      901 agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca
      961 actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca
     1021 gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat
     1081 tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat
     1141 cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatagattta
     1201 tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca agcaaacgga
     1261 ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga actgatgaat
     1321 aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc agacaatcgt
     1381 tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa agaagagttg
     1441 atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt atcggttaca
     1501 atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga ttttacgatt
     1561 atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc actattacat
     1621 gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacgaga agaaagcttg
     1681 tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt aaaacaaaca
     1741 agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa aacgataagg
     1801 caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt agagcctaaa
     1861 ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca gaaggaatca
     1921 gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat
     1981 ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg aggtattcct
     2041 tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata cgctaatcga
     2101 aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat agtcaaataa
     2161 gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa aaatgttaga
     2221 taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa atgttccaat
     2281 cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg aatctgcttg
     2341 ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac ttgagtattt
     2401 tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt ttgtagagac
     2461 aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa gagagcaagt
     2521 gtatccggtt tttcattggt tagcagtgaa accagattac caaggaaaag ggctcgcaaa
     2581 agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg gaccaatcta
     2641 tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa tgggttttac
     2701 ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag ttatgcaggt
     2761 tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt gggataaaaa
     2821 acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga taact
//
LOCUS       DAYYOA010000190         2791 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000190 DAYYOA010000000
VERSION     DAYYOA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.196"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..222
                     /locus_tag="ACHBKA_002790"
     CDS             37..222
                     /locus_tag="ACHBKA_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9446408.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            234..482
                     /locus_tag="ACHBKA_002791"
     CDS             234..482
                     /locus_tag="ACHBKA_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446409.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            513..692
                     /locus_tag="ACHBKA_002792"
     CDS             513..692
                     /locus_tag="ACHBKA_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446410.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(735..1205)
                     /locus_tag="ACHBKA_002793"
     CDS             complement(735..1205)
                     /locus_tag="ACHBKA_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446411.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1292..1990
                     /locus_tag="ACHBKA_002794"
     CDS             1292..1990
                     /locus_tag="ACHBKA_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM9446412.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2168..2509
                     /locus_tag="ACHBKA_002795"
     CDS             2168..2509
                     /locus_tag="ACHBKA_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446413.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2502..>2791
                     /locus_tag="ACHBKA_002796"
     CDS             2502..>2791
                     /locus_tag="ACHBKA_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9446414.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1026 a          403 c          564 g          798 t
ORIGIN      
        1 gcttacagat tattttttta gaaaggagct atttttatgt ctgagaattt agacttaaaa
       61 attcgagcgg agatgagaaa aagaagaatg actttcaaag aactagctgc gcttatcggt
      121 atttcaggag cttatttatc agatattcta aacggcaatc gtgatggaaa gaaagcacaa
      181 cagcatatcg aaacagtgaa aaaaatattg gacatccgat aggggggtag gcgatgacaa
      241 aactaaaaaa acaagatttt gtaaaaaaat acaattattc tccatctact tatcaacgtc
      301 gaatgtcgga actaaaaaat acagcaattt tctcagcggc gtatgaacgg gtcacaggac
      361 aagaagtttg gatcaataca gaattatacg ataaattttt gtctttcaaa tcctataaca
      421 ggttacgcac aagaaaggta acacctaaag aatttatcga gaagcattta gttgatttat
      481 aaaaaacaga acattttgag aggtgaaggt taatgggtaa attcaacaga gcattggtat
      541 tcagcgcacc gctaatcatc tacgctttag gactttgggg aagcaggcaa gcgttgatag
      601 gaacgattgt ttacatggtt tggattttta tggggcttga tgaagctgaa gctgagtaca
      661 gagcgaaaaa gccaaccgag ggggctgact aaaaatgaat agaaaagaga aactagaatg
      721 tatattacta ttactcagtt taattctttc actaatttct ctattgggaa gtttgtattt
      781 ttgatatcaa caattttgtg gatttgggtt ctatttagag taaatatcat ttttgtttgg
      841 tgattaagga gtttattaaa attaattttc cctacttcaa atgcaaggta acaagatata
      901 gcacttttag gaggaatatt tagcggtaca gcttcagagt atattctatt atcatcgact
      961 gatcctcctt tagcaattaa tttcttaaat ggagtagctg accatctgcg gtttaagtta
     1021 gcggatgaaa gttctaaatt aactaacata gctggttctg ttgaatagtt agaaatgatt
     1081 acttttgtat aaatcatatc tgcagcgaag taagaagcat taagttcaac ttctagttga
     1141 gggcgcattc cttttatttt tatccctgtt aaaaacgtac tcaatataaa acttattatt
     1201 gacatccatt gaaaaattgt taagtgaaaa aaattcaaaa taaacaccac cagtttttaa
     1261 ctaaattata ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa
     1321 agaagctgtg actacgagct tacaagtggc agaaagcttc gagaaaaaac atcaacatgt
     1381 tttaagagat attgatgcac taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga
     1441 aggtaatgaa ccagattcat atggcagaaa tcgacgagtt ttcttcatta gtagagatgg
     1501 atttttcttg ctggctatgg ggtttacagg aaagaaagct atctacttca aacaaaaata
     1561 cattgaagca ttcaacgaaa tggaagatgt tattcgcaag aatactgttc ctcaaacaat
     1621 tgaagatatg atgatctatc aattagaaga aatgaaagat gttaaaaaag atgtttccat
     1681 gcttaaagat actatgcgaa ttagcggaca acaagagttt gaaattaagc aaaaaggaaa
     1741 tatgaaagtt atgggagttc tagggggaaa agaaagccga gcttatgaag aaatcagcaa
     1801 aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc caagatatgg
     1861 cgagttacct cgtaagagat tcgatgatgc tgtttcattt attgaaatgt ggttgccaga
     1921 aactgcgatc cgtatggaaa ttgatcaact gaacagacaa cagagacttt ttggtgatga
     1981 caatgaatag agctgaagcg ctaagaatag ggacggcaat tgctaattgc tggtggaaat
     2041 actataaacc aatcatccta agccaacaac atattgacaa gcaaaaagca tggcaacaaa
     2101 taaaaaagtg actccgccgg caagcaaaga gtcacaaaga aaacacatca taaggagatt
     2161 ttagcatatg gaaaaagaac tttccactct agatcaatat ttgattgatc ctgattgggg
     2221 caagccgaaa attgaggaaa caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga
     2281 agaactagct tgggatcaag atgatttagg caaccatgta actatttggg atcatgttta
     2341 tcttatccat ctatcgaagc attcgaataa acctgaatat atttacgtca tcgaagatgg
     2401 cttgattgat gcgctagaag agtacgacag agataacttg attgatatct cttattacgg
     2461 accaggtaag aaatacattg ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga
     2521 caacgaactt tgaaaaactt tttagtcgta agttaaataa aattctcaag aaaaaaggaa
     2581 attttgatta tttatcttgg gctcacgcgt gggagattat gaaaaagaat gatccacagg
     2641 caacggtaac tattaatgag tacaaacact acagggttgt ttctggaact catcaagact
     2701 ttcttgttga ggaatataaa ccttttctta tggatgaaac tgggacttat gtatctgtct
     2761 cagtaacggt taaaggacac acggaaaccg a
//
LOCUS       DAYYOA010000191         2740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000191 DAYYOA010000000
VERSION     DAYYOA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.197"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..262)
                     /locus_tag="ACHBKA_002797"
     CDS             complement(59..262)
                     /locus_tag="ACHBKA_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446415.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(272..694)
                     /locus_tag="ACHBKA_002798"
     CDS             complement(272..694)
                     /locus_tag="ACHBKA_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446416.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            932..1546
                     /locus_tag="ACHBKA_002799"
     CDS             932..1546
                     /locus_tag="ACHBKA_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9446417.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1711..2535)
                     /locus_tag="ACHBKA_002800"
     CDS             complement(1711..2535)
                     /locus_tag="ACHBKA_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446418.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(2559..>2740)
                     /locus_tag="ACHBKA_002801"
     CDS             complement(2559..>2740)
                     /locus_tag="ACHBKA_002801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446419.1"
                     /translation="FLYIGGEVMEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIE
                     FIDSDINVFFILKKN"
BASE COUNT          902 a          455 c          392 g          991 t
ORIGIN      
        1 atgaacactt tcaattagaa aaaaagaatg tcgactagac actctttttt taaaatcatt
       61 atttttcttt ttgatatgtt ttttggtctc ttttttttct tttttcttca gaatcttcta
      121 gattagaaat tgatttttga attttttgtg tctcctgtaa agttcgctga acttgtaaag
      181 ctgattcttc tttcggtaaa ttaattttac ttagaatatc gctatcttga taaaaatttg
      241 gtgtgttcac aaattctggc ataaaaaatc cctactttct agttaaggtt cctgaataag
      301 tcgcaatcca attgtttcca gctgatttat aactttgtaa atacaatttt ccatagtacc
      361 aataaccatt tcttttttct ttccaattcc gtgattgagg aggtctagaa ggccaaccaa
      421 ctttatcaaa aatcatcgtt cctgtcactc gtatcgtttt agaagctgct agagcaagta
      481 atggagaaac tattaaataa ttttcagtaa cagttaagtc gttagttggg ttaccaatgg
      541 ctattccgtc cctatctaaa ggagttaatt cttggttatt atctactaca gtagttggtt
      601 catttgtact atcagcaaat gtaggaattg tagttccagt tacactaatt ccaatagtta
      661 aaatacctaa agataaaata aacttatttt tcattgtcta tcacctcgaa atcaaaacta
      721 agaaaacgtt ttcttaataa atcttaacag ataaatcaag ttatatacta gaaaattcat
      781 aaaatatatt tttaatgtca taatcgttta aattttcttg attgacattt atttacaaaa
      841 aatattgcca tttaaaaaga aaagtgacat atggcttcct cgtgtatgat tagggtgtat
      901 tttaaatagt tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc
      961 gatcgatcaa aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat
     1021 tttcatcgag aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt
     1081 agattacgtc cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa
     1141 ttatgacgag atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat
     1201 tactagttta ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa
     1261 ggatctgatc atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg
     1321 tcgccaggca caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa
     1381 attatacagt gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga
     1441 agatcttaaa aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca
     1501 aaccgtgtat agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct
     1561 tttattatct tctcatcttt aatttcctac atataattac tatctataaa attaagtctt
     1621 actcagaaca gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga
     1681 gtctgttaat ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt
     1741 atctatattt tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta
     1801 tgtcgacaca ttggttatac taattcctat agctagttct tgagactcta cttttctctc
     1861 tagctccttt gacaaagtga tttgagattt atttggttcc gttggagtca attctataga
     1921 ttcttcataa aaatctctat acataaagct atcattaatt ttataaaaaa aattaaatgt
     1981 ttttagcact gccatataac attcaaaaca ttcttctata gttggtttac ttccttgagg
     2041 agaatgaacc aatttattac gtttttctat gaaattttta atttcctttc ttatttcttt
     2101 cggaaactca atattaaagt accctttaaa catgctccct aataatttgt ctacaggggg
     2161 agattgtaaa ttctcaaata ttagagttaa atcaggtttc tcttgttgat aaaattcctt
     2221 tacccctatt tcacaggctg taattatcga aataaaccta tctctagtat ttgatatatt
     2281 tttacttctt ttaaggagta taagagaaaa ggggatactt tcgtgtccat gcatgtaatt
     2341 aactaattgt tgagcttcag aagttatatc tctaattata tagttaacgg aattaggaag
     2401 tgtctttaac tctaatgaaa tttctatcca attactatct tctaatctgt atatgctcga
     2461 agtataattt gattcgtcag caagcatcgc tacatcccaa acactgatca taaaatcttt
     2521 taaacgttca atcatttcag caatattgct aaaatcagct aatttttctt tagaataaaa
     2581 aaaacattta tatcagaatc aataaattct atttttttta ttactatatc gtcatcatta
     2641 tcttgaccat ttatgtttat tgttccctga gtggggagta cttctttcaa attcaagtta
     2701 aatttatatt ccattacttc tcctccaata tataaaaatt
//
LOCUS       DAYYOA010000192         2670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000192 DAYYOA010000000
VERSION     DAYYOA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.198"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(261..545)
                     /locus_tag="ACHBKA_002802"
     CDS             complement(261..545)
                     /locus_tag="ACHBKA_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFM9446420.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(566..1936)
                     /locus_tag="ACHBKA_002803"
     CDS             complement(566..1936)
                     /locus_tag="ACHBKA_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFM9446421.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            2155..>2670
                     /locus_tag="ACHBKA_002804"
     CDS             2155..>2670
                     /locus_tag="ACHBKA_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9446422.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPI"
BASE COUNT          826 a          543 c          398 g          903 t
ORIGIN      
        1 tatgtgaaaa tcgaatcttg agatagctat tccccttgag atattcactg atacttctac
       61 tcaaatcatt taaattttta taattatttg tacggaacgt atatatatca acatctattt
      121 agacgttgtc ggtgatacaa tgtcattttt cccgtgtact atgtcattta tctctatact
      181 ttgtcatttt tctttttggg caatcaaaaa aggcaacaaa ccaattatga tttgctacct
      241 tctccgttta aaaacttatc ttagccccaa aatggaatcc aatttttaac ttcttttgcc
      301 aatgcatacg ccttagcaag acctgagttt tcaactaaat atttttcccc ttccagagaa
      361 acactccccc agatatgaac agtgttatcc gcccaaacca tggattctag taagccattg
      421 cgcgaaagaa atttagtttg ttctaaaata tcttcttctg gaacttgccc tttaagctca
      481 ataaaaggat tctttttttc atcacgcaat ttaagaattg catatcttag tttgttctta
      541 tccaacctaa caccacctct atgctctatt tcttatttct tagcgaatca ttaatactta
      601 ttccgttatc atatttccag tagtcccaac cattccggct accccctaat agcagagctg
      661 ctgcattcga aacagatgag gcagtgtaat cttgaacaaa ttgtagtccc ccaattgttt
      721 cttcaatgat tccattatcc aacagctctt ggcgagtttc atctagcgaa tcagatgcaa
      781 tatctttaat cttagaataa atttctgaac ctgaatgtat aatcacggtc tcgtcttcgt
      841 tcaaataccc agttgctttt attccttgcg cctctaagaa aatttcccta ttcccatcgt
      901 gttcatagtc gcccgacgat ctcaaatagg gatacatagt aatcagttca ttgattagag
      961 cttcacttct ggcagaaata tccgatgatg tccactcagt caattcgtac aaatctttat
     1021 tcatacgaat atgaagggta tcttcaaaga ttttcttttt ggtctcaaag tctttatttc
     1081 ccgcggccga attatcagga tttgtaacga gtgttaaatt accaatggta tttactaatc
     1141 ctgtgtattc ttcccccgat gtaccagcct tctcttccca atatgaagta ctagtctgtg
     1201 gcatgatgtg ttcaatggac aagttactca tatcaagtgt tgctgaattt tctcgattct
     1261 ctattttttc aagtaaccaa cgggccaatc tcatcgcata agcattggca ttcaatagat
     1321 tagatttcaa tgccttgtca gatggaagtg ccatattatt tgattgattt ctagtaacaa
     1381 gaacataaat tacaatatca acgatatttt catatccatt agctttagcg tacttcctaa
     1441 cacttttcaa atatgaagga aatgcttttg acactctgct tgtatcatcc ccattaaaat
     1501 atctcctaag ctgataatca ttcactacct taatcgcatc aatatattgg tcttcagtga
     1561 taaattggtc atgatagtac cattcagata atcccaaaac aaaaggtgca ggcatcatac
     1621 tttccatact ttggaaatcg ctcaatactt ctgagtattt tccttcaggt ttgttatagt
     1681 aaagtctcgc aaaataacca gcatatcgca ctaatttttg gagcaattcg gcatcacttg
     1741 atacatcgcg ctctttatgc caatagtctt tgaatgcttg ataaagaata tgctttgctg
     1801 aatactcccc agttattgca gcaaggtagt atctgaaaaa gtcttccatt cctttagaat
     1861 tatcaaagac ttcttccaac ctacgccaat atttagtata aagcgtagtc tgctcttcat
     1921 tagatttatc catcatgatg aagtttcgaa tcaaatccga ggcagtcaat ctttcaccgg
     1981 tagaattgat actttcaaaa atttgctgtg cattatcacg gtcatctaat ttaatataaa
     2041 caatacgcgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa
     2101 tcaacaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact acatatgcgt
     2161 tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga aattatgaga
     2221 actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg aagaaatact
     2281 gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg cataattaat
     2341 ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt tcttaatggt
     2401 gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta cactatcaag
     2461 attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg aattatagat
     2521 agttttccta gtcaattatg taaaccagtt agaaatagac aagcaaaact attgaatcaa
     2581 atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct taaaattcat
     2641 atgatcgtta ctaagactgg ctttccgatt
//
LOCUS       DAYYOA010000193         2597 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000193 DAYYOA010000000
VERSION     DAYYOA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.200"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..293
                     /locus_tag="ACHBKA_002805"
     CDS             3..293
                     /locus_tag="ACHBKA_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446423.1"
                     /translation="MLFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLVMMLSY
                     KGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
     gene            457..711
                     /locus_tag="ACHBKA_002806"
     CDS             457..711
                     /locus_tag="ACHBKA_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446424.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            781..1026
                     /locus_tag="ACHBKA_002807"
     CDS             781..1026
                     /locus_tag="ACHBKA_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9446425.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1102..1467
                     /locus_tag="ACHBKA_002808"
     CDS             1102..1467
                     /locus_tag="ACHBKA_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM9446426.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1528..2091)
                     /locus_tag="ACHBKA_002809"
     CDS             complement(1528..2091)
                     /locus_tag="ACHBKA_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446427.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          764 a          468 c          412 g          953 t
ORIGIN      
        1 cgattttatt cgatttagtt gcggcagcag cttattttct ccaattaaat tatcaaacct
       61 caggaattct gtttattgga atgatttttc aaggtgtgat tacccttttg cttcttgtca
      121 tgatgctcag ctataaagga aaaagatatg cccgtattca aacaaaagta tttgttaaat
      181 atgtttccat tcgctatggg atcatcattc tttccttttt agtgaatgcg atcgtcctct
      241 ttttatatgt attgaattac ttagatatca atccattgat tttttctcga tagataagac
      301 acattcagcg gaacagttcc tgcttatcag cagaaaactg ttctgctttt tcatttttca
      361 ttgatctatt cttcttttat tttcctagca tttcatttgt gatgtatgca ataacatgtt
      421 atcttttatt atatgaatcg ttgaggagtc cgttacatga ttacgttggt gagagttttg
      481 ttttgggtgc ctgcggtcgc actggtcgca agtattgttt atcttatgaa ttggaacaaa
      541 gaacggtttt atttagctat tttgactctt ccggctatct actttatgtg gaaagttttt
      601 aattacaact actttgaacc cgatagtgtt tttatagaag aattatcagg actggttttg
      661 tctttattga tcgtcattct ttatttgata cgtttaaata aaaaacattg agtactattg
      721 attgcatgtg tagctttcta tagtcattat aactattcta ctataatgaa aggtgattaa
      781 atgagtatcg aagaagctat tttactggat actttacgtt gtcgtctgaa tttcttaaga
      841 actggccgat ctttatctca agtagctgtt tctagcgcgt taagtattcc tttattcaaa
      901 tatcgtaagt acgaagaagg agaagaaatt ccaagcatca ttgaattaat ctatatcgct
      961 gacttttacg atatttccgt tgattaccta attggtcgct cagaaacagc aagcaaactg
     1021 aagtgatttt ttccagcctt ggtgctggtt ttttcattta acgtctaagg ttaacaatct
     1081 atatataaag gagacatttc tatgcgatta tggcatgaaa aattgatttc aaagcttcct
     1141 cgtcaacagc ttttaggaca acatcgggaa tgctgtgcat taagaggaaa tggctgggga
     1201 aagaaacatg ctactgtgga ttatgttttt cactattctc cttatagatt attccaatat
     1261 catcagcttg tcatggatga aatggaaaaa cgtggttaca gcccagcacc agagtggaaa
     1321 aatcctatgt atcgtggcaa atcatgcgaa gcattttttg atttacctac tgtagagcgc
     1381 aacgtgccga tttatccgga acacgatgaa gtttatctag cagaatgttt agcaaatttg
     1441 gagcaaaaag gaatttatct tacctaatat aactagaaaa gccttctatc tcctcttact
     1501 tcattaaaaa gctttaacca tcttagatca agctaaaatg tcgtaagttt tagggttatc
     1561 ttcatcctca cgtgcaaaac taaaagcgta ctctagtttt ggttcatcgc caacttccct
     1621 agacataaat gaacatctcc tgacgtggtt taaaagtacc gcatcttcga cgtctgtata
     1681 cgatgcacag cacagattgc ttcttttgat ctttcttttg atttttctgt tgcttcttct
     1741 acaccttctt cgattactac atcgatgctt acaattgctt ctgcctaata ttcccatgca
     1801 tcgtcgattt aagcctctgt agtcactgtg atcgtttcca cattttaact cctctgtcgt
     1861 ggcttcagtt gattttgtga tcagttcagt tgctaagcca gagtcagaac aacctgccat
     1921 tgctagcgtt aatattgata gtaaaaatag tttttttatc ataaggttcc ccccctgaaa
     1981 atagactaaa ataataacgc tttctttttt tgttttcaga cgaaaattaa taatttctaa
     2041 atattcttta aaaaagctta taactattat gcctttttta atatataaca cagttatttt
     2101 taaaaataag aatgctaaat cactctacaa taacggcgaa agcacaaaaa ataaaaaaat
     2161 tattacgtta ttaaaaaaac agatgaaaga tcaggtttta ttagatctca ttctttcatc
     2221 acttgaaggg atttaatgaa ctactttttc ctctttgtcg tttttatata ttcgtattcc
     2281 ttcaaacagt aggatagcct ctttcaatgc ttgatatttg aactgtccgt tacttaaaaa
     2341 tttagatgtg ccgccctctt cctagaacat attctttttt actttttcat taattgacag
     2401 accaatcgaa cgaaactgta aatttcgata ggataatgat tggtcaaact cattcatttt
     2461 caagacatct cgattcatat tcaagatagg aatcttttta tcaacatcac tttattcgta
     2521 ttttcctcta ccataaaagc ttcacgctcc acaaaattgt tttatacact actattcagc
     2581 ccattcaacg accaatg
//
LOCUS       DAYYOA010000194         2575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000194 DAYYOA010000000
VERSION     DAYYOA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.201"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1307
                     /locus_tag="ACHBKA_002810"
     CDS             90..1307
                     /locus_tag="ACHBKA_002810"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9446428.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            1320..>2575
                     /locus_tag="ACHBKA_002811"
     CDS             1320..>2575
                     /locus_tag="ACHBKA_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9446429.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT          897 a          431 c          533 g          714 t
ORIGIN      
        1 agaaaaaaaa cttttagaag ctatctcttc cctgcaaaca acacctgaaa atgaagcgtg
       61 gttacaaggt gcgctggagg tgttcaaaca tgagcaatga tacacaacca gaaatccgtt
      121 tcccaggatt tacagatgat tgggaacaga ggaagttggg ggacttcgtt gtagattatg
      181 ttgaaaagac aagtgtacaa aatcaatttc ctatgctaac ttcatctcaa caaaaaggga
      241 tagttttgca agaagactat tttgcaaatc ggcaagtaac tacagaaaat aatattggtt
      301 actttgtact acctagagga tatttcacgt tccgtagtcg aagtgataat gatgtgtttg
      361 tgtttaaccg taatgacatt attgatagag ggattataag ctatttttat cctgtattta
      421 ctttaaaatc tgctgattcc gacttcttct taagacgtat taacaatgga atccaacgac
      481 aattatctat tcaagcagag ggaacaggtc aacatgttct gtctctaaaa aagtttaaaa
      541 atatagtagc aatgtttcct agtgaggaag aacaacaaaa aatcggcacc ttcttcaaac
      601 aacttgacga cacgatcgct cttcatcagc gtaagttaga tttgctgaag gaaacgaaaa
      661 aaggattctt acaaaaaatg ttccctgaaa acggagcaaa agtgccagaa atccggtttc
      721 ctggttttac ggaagattgg gaagagcgta agttgggaga attaggtaaa actcaatcgg
      781 gaattggatt tcctgatgcc gagcaaggtg gttctgaagg tactccattt tttaaagttt
      841 ccgacatgaa taacattggt aatgagtatg aaatgagaaa tgcaaatcat tatgtatcta
      901 atgagcaaat tgagcgtaaa aaatggaagc caataaaaga tgtgccagcg attatatttg
      961 ctaaagttgg cgcagcaata atgcttaata gaaaacgttt ggtcacaagc cctttcttaa
     1021 tagataacaa taccatggct tatttattta ataatacttg ggatatctat tttggtaaaa
     1081 tattgtttga aactataaat ttgccaagat actcccaagt aggggcattg ccttcttata
     1141 acagttctga tattgagaat atcagtgtta aggttcctgt aaaggatgaa caacaaaaaa
     1201 tcggtacttt cttcaaacag ctagatgata cgatcgctct tcatcaacgt aagttagatt
     1261 tactaaaaga aacgaaaaaa agcttcttac aaaagatgtt tgtctaggag ggaagaagaa
     1321 tgaaaattag tcaccgtgag gaagcagaag tcgaagaaca gttgattcgt gtacttggag
     1381 aagggcataa tcagtggacg tatcgtccgg atctaaaatc agaagaagac ctttgggtca
     1441 atttacgcca aaaaattatc tcaaataatc aagcagaact gaatgatttt cctttgaccg
     1501 ataaagaatt tgaaacgatc aaaacggaat tactgctgcg aacgaaaacc ccatttgatg
     1561 ctgcgaaatg gttaaaaggt gaaaatggga tggctcggat cacgattgaa cgggaagatt
     1621 cgcaattggg ttctgtctca cttatccttt attccaatca agacattggt ggaggaatct
     1681 ctacttatga agtggtccat caaattgcaa aacgtggaag taacatagaa gcacgggatc
     1741 gacgttttga cgtcacctta ttaatcaatg gattaccgat tgtccaaatc gagctgaaac
     1801 aagtaactgc aaaagatggt gtttatcagg catttaacca aatcaaaaaa tatgcagaag
     1861 aaggtatgtt tcggaacaat atcttttcga ccttacagtt gtttgtggtt tccaatgaac
     1921 aaaccactcg gtattttgca aatgccttac cgaaagactt gcaccctaag tttttattta
     1981 gttggcgaac gaaagacaat gaaaaagtag aaaacttata tgagttttgt aaacaagtgt
     2041 taaatatccc ggatgctcat cgtttgattg cggattatac aatcgtcagt gaagatcaag
     2101 acaataaaac cctcatggtg ttacatcctt atcaagtaca tgcgattcaa gccttgttta
     2161 ttgccgcaaa taaacatcaa tcagggtatg tgtggcatgc gactggttca ggaaagacgt
     2221 tgactagttt tgtatctact aagcttttag cacgaaaatc ggggatcgat cgaacgatca
     2281 tgttagtgga ccgcaaagat ttagataatc aaaccaccac agagtttaca aaatttgctt
     2341 cggaatttaa tactgggatt tcttccggga acgccaaagc gaatagcttg atcgttggaa
     2401 cgggaagtgc caaagaatta agtgaaaccc tgttggcaga tgccaatgca aatgttgtaa
     2461 tcattacaac ccgtcaaaaa ctagatgcgg cgttgaaata tgcaaagaaa caagaagaga
     2521 aaaaaggaac caatcgcttc caaaaattaa tgggccaaca tatcgtgttt gtagt
//
LOCUS       DAYYOA010000195         2574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000195 DAYYOA010000000
VERSION     DAYYOA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.202"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACHBKA_002812"
     CDS             complement(<1..103)
                     /locus_tag="ACHBKA_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9446430.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRG"
     gene            173..523
                     /locus_tag="ACHBKA_002813"
     CDS             173..523
                     /locus_tag="ACHBKA_002813"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9446431.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            937..1755
                     /locus_tag="ACHBKA_002814"
     CDS             937..1755
                     /locus_tag="ACHBKA_002814"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9446432.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1828..2187)
                     /locus_tag="ACHBKA_002815"
     CDS             complement(1828..2187)
                     /locus_tag="ACHBKA_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9446433.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          846 a          374 c          474 g          880 t
ORIGIN      
        1 gtccacgttt aagctgcttc gcaacgatgg caaccggagt ttcttgatga aaatatgctt
       61 ctatcattac aagctcgttt ggtgtaagat gggtataagt catttatgtt cactctcctt
      121 gtatgcttta gcgggtatta caatttgagt gtaacataaa tggctttttt atttgtctcg
      181 cttaattata caaacggcgt tttataaagc aatcattcct gagaacttag atgaggttaa
      241 actttattta tgggaagata acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga
      301 attagttatg ctatttcttg atccagattt tattggtaag cgatatggta gtcaaatatt
      361 aattgagctg attgaaactg aagatataaa aagaattgat gttaatagtc aaaataaata
      421 tgcaaaacat ttttatttaa gccatggatt tgaaattgaa tctgaagaag acttagatgg
      481 ttttggtaaa ccatacccta taacgcattt agtaaaaaaa taattcatga atggtttatt
      541 acctgattga acatgaataa aagtaatatt tttattttga tggcctattt ctaggttatg
      601 aattatccag atcaaatttg tgtattacat tcagtaataa ggtgcttatc ttaggatcaa
      661 cttatgatac tagctgacca aggatgtacc agttatagaa aatcagttaa agtaaaaatg
      721 ataaaaatct tatgtcaaca ctgatttagg ctgacctgtg ggttaggaga aagacaggtc
      781 ggtagctttt aatacaaaaa atggcttgta tgtatctaga tagttaactg aaattagaaa
      841 tgttcttatt tttaacggag taagaaaaat aggctgcttt aggtctgaat actttttcaa
      901 tataatgaag atatcaaagg cgacaggagt gtttttatga ataagaatcc tcataatcta
      961 aagttggtag cagtagatat ggatggaaca tttgctcgta ctgattacac atacgatatc
     1021 ccaaggttca aggctgtttt atcgcgaatg aaagctgttg attgtcaatt tgttgttgca
     1081 agtggaaatc aatactacca attgagatcg ttatttccaa attattatga tgaattaagt
     1141 tttgttgctg aaaatggtgc ttttgttaaa gataaaacag aggtactatt ttcagccgat
     1201 atgcctagaa aagcgatcga ggtggtcctt gatttatgta aagactatcc tgaagtaaaa
     1261 aatgtgatgt gtggtctgaa tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt
     1321 tttgatctaa ccaatatcta ttaccataga ttagaatggg tagctgattt tgcagaagtt
     1381 caagataaaa ttttaaaatt tgctccgact atgcctgttg aaaaaaccga attttacgtt
     1441 gaattgttcc gtgaaaaatt agccgggcta ttagtaccta ccagtagtgg tcatggttct
     1501 gtagacttga ttctttctga gtgccataaa actttcggct tgaaaatgtt agttgagcgc
     1561 ttggggatta atcctgacca gtgcgttgct tttggagatg ggggcaatga cattgagatg
     1621 ttggagtatt gtggtctgag ttatgaaatg gataatgcaa cggaagctgt taagcaagtt
     1681 gctaagcatc aatgtccttc aaatgacgaa gacggtgttt tagtgacgct cgatcgattg
     1741 ttccctaaca cgtaaacact aggtaattca tcaaaaaacc tcgatataag aaatattcga
     1801 ggttttaaaa ctatatttag aacagattta cgaagcgtct gatgagtcaa aatagacttg
     1861 gacattgcta tctaaaatac cactttcatt ctttaaacga attgtttgat ttgatggagt
     1921 tacctctaat actaaatgcc cattaataaa ggtagtatcg tattcagatg ttttaactgt
     1981 tgcttcttcg caaggtattg ttaaatcata tgctggaata taatgttgtg tggaaactaa
     2041 taaaaaatga tcaccaatca tacagcatcc ctctgctgaa gaaaattgat tagagccatc
     2101 atttaaagtt aacaagaatc catcctttaa acgaggtact tgcttaaata cttctggttg
     2161 tacttctaaa aataaattat cgctcatttt cacgaccacc atcgacttcc acagttgaaa
     2221 ttgacatttt atttaacaca cctattcccc cttaactata actcaattct atataaaaaa
     2281 tattctattc tcaaataatt gaacttttca aatcgccaat ttacttcggc tttatcagag
     2341 ctcattcata tcaagagcga ttaacgctgt aaaaattaaa ccgaagatct gataaatttt
     2401 ctctattttc tacaatattt aaaacgcttc cttagtctat tatatatcta tttctcttag
     2461 gagtatgttt agatgtttga ataatagttg cccaacttta aaggttattc ttataatatt
     2521 ttacttcatt ttccaataga aaattcaaag gattactggt ttttctaaaa tagt
//
LOCUS       DAYYOA010000196         2491 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000196 DAYYOA010000000
VERSION     DAYYOA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.203"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHBKA_002816"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHBKA_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM9446434.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          702 a          501 c          384 g          904 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt t
//
LOCUS       DAYYOA010000197         2434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000197 DAYYOA010000000
VERSION     DAYYOA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.204"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..2284
                     /locus_tag="ACHBKA_002817"
     CDS             131..2284
                     /locus_tag="ACHBKA_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM9446435.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          832 a          427 c          538 g          637 t
ORIGIN      
        1 ctgtgaaaca aaaagttgat tttatcttga aattaggtaa agaatttctt gctccgccca
       61 ttttttcttt tttattaaga agtttttggg tacaatgaag catgaatgta aataggcgga
      121 aggataggaa atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa
      181 aataagaaaa ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt
      241 tcgcttaaat tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt
      301 gggttatttg cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac
      361 gattacggtc caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt
      421 agttacgaat aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa
      481 agatttgctt gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa
      541 tctgacagat agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc
      601 cacaaaaaga ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat
      661 ggtcaataaa gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac
      721 catctttaaa cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg
      781 tgttactgat caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc
      841 gactggcaca gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg
      901 cagtgtgacg actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa
      961 aggttattct cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt
     1021 cctgcaagga accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa
     1081 tcaaaaagaa atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga
     1141 attccaatca aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg
     1201 caaagcacag tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt
     1261 gctagcaatg actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt
     1321 aggcacgatt actagcgcct ttgatccagg gtctgtagtc aaggctggta ctttgacagc
     1381 aggctgggca agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct
     1441 gcaaggtgct tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc
     1501 agtcaaagca tcggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt
     1561 aggactagac tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta
     1621 tcagcaattg agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt
     1681 gccgaatgaa tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga
     1741 taatggcaaa gaatggtatg ctccaggtaa ctttaccgat ttggcatttg gacagtttga
     1801 cacatataca ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg
     1861 agtacagccg cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga
     1921 agtgaagaaa gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat
     1981 ggcgatcctc cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacaacagc
     2041 caaaccatta gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt
     2101 tgcggaaggt catccagata ttgcgacagt caatagtaat atcgtggcat acggaccaac
     2161 agacaatccg gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca
     2221 cataaacttg acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa
     2281 ataaaaaaga ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa
     2341 atagcttctc acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac
     2401 cgtttactcg gcaaaaaagg ttgtgacaaa aatt
//
LOCUS       DAYYOA010000198         2407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000198 DAYYOA010000000
VERSION     DAYYOA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.205"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACHBKA_002818"
     CDS             5..1150
                     /locus_tag="ACHBKA_002818"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM9446436.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACHBKA_002819"
     CDS             1321..1779
                     /locus_tag="ACHBKA_002819"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM9446437.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACHBKA_002820"
     CDS             1938..>2407
                     /locus_tag="ACHBKA_002820"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM9446438.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAYYOA010000199         2390 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000199 DAYYOA010000000
VERSION     DAYYOA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.206"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACHBKA_002821"
     CDS             197..2059
                     /locus_tag="ACHBKA_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9446439.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACHBKA_002822"
     CDS             2059..>2390
                     /locus_tag="ACHBKA_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9446440.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAYYOA010000200         2373 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000200 DAYYOA010000000
VERSION     DAYYOA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.207"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(172..2256)
                     /locus_tag="ACHBKA_002823"
     CDS             complement(172..2256)
                     /locus_tag="ACHBKA_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9446441.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          689 a          527 c          362 g          795 t
ORIGIN      
        1 ttcatatttt ttccatcaaa aaaataagtt attttctttt atttcattga ccttattgaa
       61 tttcaagcta ttttgaattt tctgcaaaat taaaaacatt agttatttta taatcctatc
      121 tattttcttt acatttttca ttaagaaaag gatataaaaa aaagaggacg ttcattcgtc
      181 ctcttttttt acatgcaagc ttcgcatggc attcaaaaca gctaatactg tcaccccaac
      241 atctgcaaat actgcatctc ccattgatgc aaaaccaaaa gcacctaagg caagtaccaa
      301 aatttttatc ccaatagcaa agacaatgtt ttgtttgacg attttcaatg ttttctttgc
      361 taaatggatt gcttctgcaa tcttcgatgg ttgatcattc atgatgacta catctgctgc
      421 ttcaattgca gcatcgcttc ccaatccacc cattgcgatt cctacatctg ctcttgctag
      481 caccggtgca tcgttgattc catctccgac aaatgcggtc tttttattgt ttctttgtaa
      541 gatttcctct aaccgctgca ctttatcttc aggaagcaat tcgctatata tcttgtccac
      601 gcctacttgc ttcccgatat gatcagcaat ttttttcgag tcgcccgtca acataactgt
      661 gttcttcaca ccagcctgtt ttaattgaac caatgcgtct acggcatctt ctttcaaggt
      721 atctgcaata acaagataac cactgtaact ttgatcgatc gctagataaa gcaatgtacc
      781 gatttcttga cttaaaggaa aagagatttc aaatctttcc atcaatttgt gattacctgc
      841 taatacttgt ttgccatcaa cttcagctaa gatcccttca cctgcaaatt cctggatatt
      901 agttgttggt aaaagcggac ctgcgtagcc ttctaaaaca gattgagcaa tcggatgtgt
      961 agaaaactgt tctgcactcg caatatattg catgaatttc tttggatcga tttttgtatc
     1021 gatcgtctgg atcgaaaagt cacctttcgt taatgtgccg gttttatcga ataccactgt
     1081 ctcggtttgc gcaagcaact ccaagtaatt tgaacctttt actaatacac cgattttact
     1141 tgcgcctccg ataccgccaa agaaactaag tggcacggaa atcactaatg cacatggaca
     1201 tgagatcact aaaaatgtca atgctcgata gatccattca gaaaacgccg ctccagaaac
     1261 aacaagtgga ggtaaaacag ctaataaaac ggccaatcct acaacgactg gtgtgtaata
     1321 gcgcgcgaat ttagtaatga aattttcagc tggtgctttt ttactgctgg cattttctac
     1381 taattctaat aatttgctga tagtagaatt ttcataagat ttttctactt gaactaaaaa
     1441 tggtttattt ttattgataa atccgcttaa tactgtttca ccaacatata cagatttagg
     1501 gactgattct cctgtaagtg ctgacgtatc gactaatgat tctccttcga tgatcgttcc
     1561 atctaacggt acacgttcac caggcttcac aagtacaaac tgaccgatct gcacgtcttc
     1621 agggttcacg gtcatcaatc cttccgtcgt ctgcacgtga gctgtttctg gacggatcgc
     1681 catcaattcg cgaatcgatt ttcttgaatg actgactgcc aatccttgga aatactcgcc
     1741 gatttgataa aatagcatca ctgctacggc ttctggatat tcaccgatca acatagcgcc
     1801 aatcgtagca acagacataa gaaaattctc atcaaagatt tttcctttga cgatattcat
     1861 cactgccttc ttcactacat catatcctat cagcagataa atcaataaat accctatcaa
     1921 gcgaattggt tcattaaaat caaataatag caataaagcg atacctgcaa gggaaaataa
     1981 aatccggaac agcgtatttc tctctttttc atttccatgt tcagatactt tttccttatc
     2041 tatttctgtt actttgacat ctggttctaa ttccttcact actttttttg tttcttgttc
     2101 aatcgtagcc aaatgatctt cctctgcgtc aatcgttagt tttccagaag tgaagtttac
     2161 cgttgcttcc ttgactccat tgatcttctg aactcctttt tcgattttca ttgcacagtt
     2221 cgcacaatct aaaccttcta atctgtaact tttcatttta atgacctcct cgcctcaaca
     2281 tatgaacatc tcttcataac taattataaa tgaatggtta ttcatatgtc aaggtatccg
     2341 aaagaaaaaa tgaaaaacat ctgcatcgga aaa
//
LOCUS       DAYYOA010000201         2295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000201 DAYYOA010000000
VERSION     DAYYOA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.208"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..351)
                     /locus_tag="ACHBKA_002824"
     CDS             complement(130..351)
                     /locus_tag="ACHBKA_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446442.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(449..694)
                     /locus_tag="ACHBKA_002825"
     CDS             complement(449..694)
                     /locus_tag="ACHBKA_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446443.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            934..1548
                     /locus_tag="ACHBKA_002826"
                     /pseudo
     CDS             934..1548
                     /locus_tag="ACHBKA_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1918..>2295)
                     /locus_tag="ACHBKA_002827"
     CDS             complement(1918..>2295)
                     /locus_tag="ACHBKA_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM9446444.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          790 a          388 c          319 g          798 t
ORIGIN      
        1 atatttatgt cctatttcaa ttatatttat atatgcttaa ataaatttgt atcctgaaat
       61 ttattgtaat atgaagctgt attttacata tagtaaccaa agagttaata aaataaaaag
      121 agcgaaccat tatagatcag ctcttttttc attgctgttt ttaatcttat ctagaatcac
      181 ttcaaatgtt tcaaaggtat ttacatctaa ctgacataac caaagcaagt acttcttttc
      241 tttgtaagtc caatcaatat tacggtaaga ggacattaga ttatggtacc ttgctctttg
      301 ttcaactgaa gccaattccc agcgtttttc aaatattttt tcttcttcca ttattgatcc
      361 ctcgcaagac gctacctcaa acttaaataa tgggcagcga aagtctaatt ccttagatct
      421 aagtatagca aacaaatatg aaaaaaactt agaaggtagt aaaagttctt ccgtcttttg
      481 ctttgcttct attcttaatg tgtttcgtta tcggtgccct acaaagattt tcattaatta
      541 cacactcatt tattgttgtg tattttctga catttctctc ttgatcaatt attttaattc
      601 catgggcatc ctgatagttc ataggagagg agcggctatc agaaatagtt gcacctatct
      661 cttgaacttc ttttacaact tctggaaaat ccatccatcc tgcttcagct gtcaaaagta
      721 ataatctata atatttatct ttccttttca atctaagaaa tcgtcacccc attctttttc
      781 ttctattata aactatttaa cttttagaat tcaaaaaccg tttgtctatt tagagtttac
      841 cctaaataga caaaaatata acattgaaaa atttagagtt ctaacgtatg attagggtgt
      901 attataaacg ttatagaaaa gtagggaata aatttgaaaa ttgcttatat tagagtttca
      961 tccatcgatc aaaatgaaca acgtcaaatt gaagaaatga aaaagtttgg cgctgaaaaa
     1021 atttttatcg aaaaacaatc tggagcgacg attagtcatc ggccgatttt ccaggaagct
     1081 ttagattttg tcagagagca agatattttt attgtagaag ccattgatcg acttggacgc
     1141 aattatgacg agattatcga ttcagtgaat tatctaaaga agaagaatgt gcgacttatt
     1201 atcaccagtt taccgatcat ggctgaagca attggaaatc ctctcctaga taaattcatc
     1261 aaagacttaa tcatttaaat ctcagccatg attgctgaac aagaacgtac tgaatcgaaa
     1321 cgacgccagg cacaaggaat taaaatcgct aaagcaaacg gcgtctataa aggtcgtcct
     1381 aagttataca gtgcagacac caaagatcct caacgtcgcc ttgtctatag gagtatcgtt
     1441 caggatcttg agaatggggt tgctatctca aagattgcaa cagactacaa tgtgactagg
     1501 caaacaattt accgaataaa aaaagaaatc gaccaactaa ttgtatgatt tcttttttga
     1561 atcacgtaat tattaattag ctttgtcatc aatacagaca atcattttag attcatctag
     1621 ctaagttgat tacatttatg ttaaaaagaa tttagttaac ataaatataa ttgatgcata
     1681 aatcgtcctt atttaaaggc atttaacatt gacaacttag tataatgtat cttatgtaaa
     1741 ctaataagat ttgaatttta ttttatacac actcgataat agatcaattt tatggtcccc
     1801 cgcatttacg attgcacaag agaaacataa agtcgaccta ttagcatttc ttttcaattc
     1861 aaacattaaa aaagtttata cctctgctac taaaaaatga aattatctat ttaaaattta
     1921 aaacggtaaa tctgattcat ccaaattgga aaatggatca gcctgtattg gagctttgta
     1981 tttctccata ataacagata tctttttttc attttttaaa aaataccttt taaatttctt
     2041 atagtcctct gaaatcaaaa tatagcgtga tggatactca tcaaaagaag aatcgtattc
     2101 ttcctttaaa attcccgatt tacttaattt ttctagtaca tcaaatactc tatcactcaa
     2161 gtaactcctt aaattgttat cactttccca ttgaactact tttctataag ttgaattatc
     2221 agttgaaaag atagaatttt catccataaa cgcatatatt agcagcaatc tttcatcatt
     2281 gtcaaattct attgg
//
LOCUS       DAYYOA010000202         2239 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000202 DAYYOA010000000
VERSION     DAYYOA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.209"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..1687
                     /locus_tag="ACHBKA_002828"
     CDS             74..1687
                     /locus_tag="ACHBKA_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9446445.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          698 a          399 c          444 g          698 t
ORIGIN      
        1 aattttccgg cacaattatt tgctgttttt taccataaca tagtacgatc aacgtagaaa
       61 aggagatgtt tttatgaaag ttgttgtcat tggcggtgta gctggcggtc cttcatttgc
      121 cactcgtttc cgtcgattga atgaagcgca cgaaatcatt atctatgaac gcggagaaaa
      181 tatttcttat gcaagttgtg ctttgcctta ttatttagga ggtgtgatca ctgaccgtga
      241 ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa aacaacatcg atgtctttac
      301 taaacacgaa gtaacagcaa tcgatccttc tactaagcga ttaacagtta aagacctatc
      361 cacaaatgaa gaaataaaaa cagattacga taagttgatc atctcttctg gtgctagacc
      421 agattatccg gatattcccg gagtttttga agcagaaaac ggctttgtcc tccgtagtgt
      481 gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa aatccacaac atgtcgtcat
      541 tcttggtgca ggtgttatgg gtctggaatt agctgagaat ttcaagcatc gcggcttaga
      601 cgtgacttta atcgatcaat tgccacaagt cgctttccct tatgatccag aaattgctaa
      661 tttagtttat gacaaattgc tgaaagaagg attagccgtt catttagaaa caagagttac
      721 tgagatccgt gataaaggtc gagaaatagt attatcagat ggctccgtcc tttctgctga
      781 tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa gtggtgaaag caaccggcat
      841 acaattatca gatacaggtc agatcattgt tgatgaccag ttacaaacca atctgccgga
      901 catctatgcg attggcgata ttatcgaaac gactagtgta gtgactggtc agccgatcca
      961 aagtatgctt tccagtgcgg ccaatcgtca aggacatatg ctggcagata ttttaaatgg
     1021 tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc gcaaaaatct ttgatcatac
     1081 agcaagttat gcgggaatga cagaacatga attaaaagca tcaggcataa caaattataa
     1141 aactgttttt atcactccct ttgaccatgc ctatttctat ccagaagcta caagattaaa
     1201 tctaaagctg atttttgatg cagatagcgg tcgtatttta ggtggacaag catttggaga
     1261 aaaaggtgtc gataaacgga tgggagaact ttctgtagca atcaccggaa acttgacagt
     1321 ctttgatttg cctgatttgg agttgcctta ctctccaccg tattctacta cccgtgaccc
     1381 gttgaatata gctggttatg tcgcaatcaa tcaaatgacg aatatcgtag aaacgatcaa
     1441 agcaagtgat atacccgaaa acgatttgaa agaagcgttc tttttagata tacgtgaacc
     1501 taataaagca ccttctggca gtatctcggc aacaaaaaat attccaatga atgagttacg
     1561 agatcgaatc aatgaaatcc caaaagataa aaaagtttac attactttca gaagaggatt
     1621 gaatacttat acttctgccc gaatcttggc aggtttgggt atcaaagcga ttttgattga
     1681 agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg agttattttt acttgaggag
     1741 taaaaatgaa aaagtatata gggttgttgg ttggtatgta tatagaatag aagataaatg
     1801 tgaagaaatt gtgcattatt tcttcacatt tattaggtta tagtttaaga aagtttaaac
     1861 attgcgcaat ttaattattt tagctgtttc ttttcataaa aaaacatgtt gtgactctga
     1921 gacaaatcat agtccatagt gatgtctcta gagtaactac gcctaatatt acgaggcatc
     1981 atattaatct atcagcacta gtacctaaac tagcatccct ttcgaatatt ttttcttata
     2041 taataaagtt tgctttcact cctctaatct caccaaatcg actggcttgc gttgtgtgat
     2101 actaggaact gattggttta gaacgacttt tctattgtaa tcatattttt cgccatcctc
     2161 gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg tattaaataa tttaattttt
     2221 ttgttcctat ttatttaac
//
LOCUS       DAYYOA010000203         2208 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000203 DAYYOA010000000
VERSION     DAYYOA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.210"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..741
                     /locus_tag="ACHBKA_002829"
     CDS             <1..741
                     /locus_tag="ACHBKA_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9446446.1"
                     /translation="DDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKILDDI
                     SFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTTKEFD
                     TDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCMLSKMM
                     LSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIAVSDK
                     GVIDRADTTYDEFLENADIQKQLNELWA"
     gene            complement(870..1733)
                     /locus_tag="ACHBKA_002830"
     CDS             complement(870..1733)
                     /locus_tag="ACHBKA_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9446447.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
BASE COUNT          667 a          437 c          413 g          691 t
ORIGIN      
        1 gacgatatcc aaccttcttc tagacgttat ccattcgttg gattcaaacc tgaacgtgag
       61 attggaaacg acttattgca ggtagaaaat gtaagtgtaa cgattgatgg aaagaaaatt
      121 ttagatgata tatcttttaa tttgagcaaa gaagacaagg tcgctttcgt cgcagaaaat
      181 gatataacaa caacaacgct attcaaagtt atcatgggtg aaattacgcc agatactgga
      241 acagtacgtt ggggtgtgac aacaagtaat tcttatttac caaaggatac aacgaaagaa
      301 ttcgatactg atctaacgat tttagactgg ttgcgtcaat atgcaagtaa agaagaagat
      361 gataacacct tcttaagaag cttcttagga agaatgttgt tttctggtga agaagtattg
      421 aaaccagtga atgttttatc tggtggagag aaagtccgtt gcatgctttc aaaaatgatg
      481 ctttcgaaag ctaacgtact tgtattagat gatccaacga accatttaga cttagaatca
      541 atcacagcat tgaatgacgg cttgatggca tttactggct caatcttatt tgcgtcacat
      601 gaccatcaat ttatccaaac attggcaaac cgcatcattg ccgtttcgga taaaggtgtg
      661 atcgatcgag ctgatacgac atatgatgaa tttttggaaa atgcagatat tcaaaaacaa
      721 ttgaacgagt tatgggcata atggcctatt tagcttaaaa aataaaaaaa cagcgacctc
      781 taagagatag gatgctcatc tatcttttaa gagtcgctgt ttttgttaca atcaatttct
      841 gggctttttt acagaatgag ttactgcttt tatcgactga ttcccatcga aaaaatcgcg
      901 atacagccat agcctgtgtg actggcgatc gttggtccta agggataaag cttcacatct
      961 ttcatcttta tctgctcttc caaaagccgt tttgccttct ccgcgctctc tagatcccca
     1021 gcgtaagcca cataaacgat ttgatcaagt ggtgcaacga ttgtttttac cgtctcattg
     1081 acgattgtct ggatcgactt gtttcttcct cgaactttgt caacattcgt cagccttcct
     1141 tctttatcca ccgtaataat aggctttaca ctcaatagac tgccaagagt agcacttgct
     1201 ttggaaatcc taccgcctcg ttctagatat ttcagatcgt ctactgttac ccatgaatgg
     1261 actttcaaat aattactttc cagccactct agggtttctt cgatgctttt accttctttt
     1321 ttcaatctta cagcttccaa tactaaaagc ccttccccaa tacttgcagc ttttgtatca
     1381 aaaacataga tctcagggtc ttcatattct tccttcagca attcaaccgc ttgaagcgca
     1441 ctactgtaag agccgcttaa tccactggaa aaagccacat ataaaacagg aatctgtttt
     1501 tccgcataag aacggaaaaa ttctaaatat cggccaacat tgatttgtga tgtcgtaggc
     1561 atcgcaccat ttttcagctg attcatgtag cggtcataat tgaatgtctt tccgaggtca
     1621 tctactaact cttgatcatt caattggaga ttcattgata aaaaatcaac tttttctttt
     1681 gctaatacat ggtatggcag atcacaacaa gaatctgtta caatttgata cacatttttc
     1741 aaccccttct cgttacattt tacccttatc gtctattata gcaaatctct tgcgctagac
     1801 acactttaac cggtttattt tcgcaagttc taatcaaaat aagaggttgt gacagaagtg
     1861 gtcagcttca agaaataaga aggaatttac gaaaattgct tctcaaattt ttgtgaaatt
     1921 tcggcttatt tccgtagaag ctacttctgg aacaccgtga atcagaggtt gtgaaatcag
     1981 cgttttgacc tgagtattag agaaaaaaac ggaaaaaata gttcctcata ttttgccgtt
     2041 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
     2101 gccgtttagc tccgagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa
     2161 tattgtgact tgtgaccgag gagttggcag gagaacaccg tttagtgg
//
LOCUS       DAYYOA010000204         2139 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000204 DAYYOA010000000
VERSION     DAYYOA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.211"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..262)
                     /locus_tag="ACHBKA_002831"
     CDS             complement(<1..262)
                     /locus_tag="ACHBKA_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446448.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFL"
     gene            complement(846..1649)
                     /locus_tag="ACHBKA_002832"
     CDS             complement(846..1649)
                     /locus_tag="ACHBKA_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9446449.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT          681 a          404 c          255 g          799 t
ORIGIN      
        1 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
       61 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
      121 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
      181 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
      241 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
      301 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
      361 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
      421 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
      481 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
      541 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
      601 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
      661 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
      721 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
      781 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
      841 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
      901 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
      961 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     1021 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     1081 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     1141 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     1201 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     1261 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     1321 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     1381 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     1441 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     1501 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     1561 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     1621 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     1681 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     1741 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     1801 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     1861 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     1921 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     1981 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     2041 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     2101 aacactatct aggagtttat tttgcttgtg taactttga
//
LOCUS       DAYYOA010000205         2129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000205 DAYYOA010000000
VERSION     DAYYOA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.212"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..>2129
                     /gene="acm"
                     /locus_tag="ACHBKA_002833"
     CDS             14..>2129
                     /gene="acm"
                     /locus_tag="ACHBKA_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM9446450.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          839 a          343 c          414 g          533 t
ORIGIN      
        1 ggagatagaa aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac
       61 taatataaca tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag
      121 taatgtcact tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt
      181 aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa
      241 ttggccaagt gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat
      301 tgaaaataaa aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa
      361 tgaaaatatt aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag
      421 aaacgtaacc gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa
      481 aacagctact gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta
      541 taaaacagga gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa
      601 caataacggc acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca
      661 aagattagat ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta
      721 tagaggagaa gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt
      781 ttcagtaact gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa
      841 aataatggtg agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca
      901 ttcggaagct tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca
      961 tacagttaaa aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa
     1021 gattttcaaa tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag
     1081 acgagcagat gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca
     1141 gggagaagct agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc
     1201 acccaattgg attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga
     1261 aaatgataca gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac
     1321 agcaactaaa atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt
     1381 cagagcaagt acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa
     1441 tggaataact tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata
     1501 tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca
     1561 aaaaattgaa aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg
     1621 acaaaaaaca tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt
     1681 aaatctatta gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg
     1741 gcgctatcgt ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt
     1801 aacggaagac aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag
     1861 ttatacaccg gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa
     1921 ttttccagag aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat
     1981 atttgctcat acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt
     2041 acctaaacag ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc
     2101 tagttttgct gtaatcattg gtttattga
//
LOCUS       DAYYOA010000206         2113 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000206 DAYYOA010000000
VERSION     DAYYOA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.213"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            727..1059
                     /gene="traE"
                     /locus_tag="ACHBKA_002834"
     CDS             727..1059
                     /gene="traE"
                     /locus_tag="ACHBKA_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM9446451.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1678..2019
                     /locus_tag="ACHBKA_002835"
     CDS             1678..2019
                     /locus_tag="ACHBKA_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM9446452.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          743 a          279 c          437 g          654 t
ORIGIN      
        1 ctattttaat agaatttata agttaaaata ttatgataaa aatcacaaac gtagcctttt
       61 acgtcataag tgttaaaaga aataattaat ttcaaatata gaaaagggaa cgtatgtttg
      121 catactcctt ttttttgagt tataataata cgcgaagaga gataatttca ttatttaaac
      181 aagctaaaaa tcttgatatt acttcttttc aggcaatacg aatattattc gcttacattt
      241 tttgtataaa tttaattgtc tgctatgctt gtgatgaatt gattttcgtt ttgcactttt
      301 ggatgataaa tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag
      361 gctggtaacc ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac
      421 ctttcagtgt tacggtatta cccgcaatgg atttagtata acagatattc aaaagtgata
      481 ccactttttt tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta
      541 agcataatgt tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag
      601 agggaacgca tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc
      661 tctatttttg aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt
      721 tattttatgt catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg
      781 tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg
      841 cagtgtaaga aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt
      901 gggaaacaaa agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca
      961 atcaaacatg tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg
     1021 cgttgtaccg caaaagggat ggggcgtttg atcggctgaa ttgatcattc tcataacttg
     1081 tattttatcg tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt
     1141 cactagaaaa ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg
     1201 taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga
     1261 tagtggtgaa atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa
     1321 gttagacaag ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact
     1381 ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa
     1441 attgtgtcgt gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat
     1501 cgagcattta ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat
     1561 tttttatgta tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt
     1621 gaaaaaatta atataaagca tagagaaaat gaaatttacg taatggagga atgacaaatg
     1681 aaaaattatg atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt
     1741 tgggattaca aaggatttgt gacgtttaga aggggcggaa attgtaaagg attagatgtt
     1801 ttagcattag acgaggatga tttatacgat caaacattga ctgataatcc aattggattc
     1861 ggcttgctac ctgaagatga tgaaggtaat gaatggttta aaatgacgct tatgaatgat
     1921 aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc
     1981 tttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta
     2041 cagataaagg cttagaagga gtcacgacga ttaataaaaa ggtagtatta ccttaggcat
     2101 tgagtaagga att
//
LOCUS       DAYYOA010000207         2065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000207 DAYYOA010000000
VERSION     DAYYOA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.214"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACHBKA_002836"
     CDS             <1..1008
                     /locus_tag="ACHBKA_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446453.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACHBKA_002837"
     CDS             1025..1174
                     /locus_tag="ACHBKA_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446454.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACHBKA_002838"
     CDS             1171..1617
                     /locus_tag="ACHBKA_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446455.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACHBKA_002839"
     CDS             1619..1756
                     /locus_tag="ACHBKA_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9446456.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ACHBKA_002840"
     CDS             1794..>2065
                     /locus_tag="ACHBKA_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446457.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAYYOA010000208         2058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000208 DAYYOA010000000
VERSION     DAYYOA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.215"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..337)
                     /locus_tag="ACHBKA_002841"
     CDS             complement(<1..337)
                     /locus_tag="ACHBKA_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9446458.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="ACHBKA_002842"
     CDS             complement(404..559)
                     /locus_tag="ACHBKA_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFM9446459.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="ACHBKA_002843"
     CDS             1176..1397
                     /locus_tag="ACHBKA_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446460.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="ACHBKA_002844"
     CDS             1398..1655
                     /locus_tag="ACHBKA_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446461.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="ACHBKA_002845"
     CDS             complement(1791..>2058)
                     /locus_tag="ACHBKA_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005727 - extrachromosomal circular DNA
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rep family protein"
                     /protein_id="HFM9446462.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DAYYOA010000209         2055 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000209 DAYYOA010000000
VERSION     DAYYOA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.216"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACHBKA_002846"
     CDS             <1..365
                     /locus_tag="ACHBKA_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9446463.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="ACHBKA_002847"
     CDS             complement(721..1293)
                     /locus_tag="ACHBKA_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9446464.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHBKA_002848"
     CDS             complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHBKA_002848"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM9446465.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          638 a          425 c          308 g          684 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctag
//
LOCUS       DAYYOA010000210         2040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000210 DAYYOA010000000
VERSION     DAYYOA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.217"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACHBKA_002849"
     CDS             <1..1872
                     /locus_tag="ACHBKA_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM9446466.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DAYYOA010000211         2027 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000211 DAYYOA010000000
VERSION     DAYYOA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.218"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1052
                     /locus_tag="ACHBKA_002850"
     CDS             30..1052
                     /locus_tag="ACHBKA_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFM9446467.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            1074..>2027
                     /locus_tag="ACHBKA_002851"
     CDS             1074..>2027
                     /locus_tag="ACHBKA_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9446468.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFH"
BASE COUNT          816 a          214 c          385 g          612 t
ORIGIN      
        1 tttgaattaa tacaaagagg ttaaagaata tgacacagaa acattcaagt ataaaaagaa
       61 ttatacatcg catatccgaa gaattgaatg caacaagacg aattgcaaag ataacatctt
      121 ggaaggaagc atttgccacg ttacaagcta aaattgatat tcaagtcatg aatcgtaatg
      181 gatataatga aagtaaaaaa cagaaggaac atttattaaa aaaacatgag gtaatgctga
      241 agtattatga aaagactttt tcagattttt tgaaaacata tgattatagt caaaatcaaa
      301 taattaatca atcaaaatca ccatattcta actgcatttg ggtttgttgg tggcagggat
      361 tggatcaatc gccggaaatt gtaaaagctt gtgtaaaatc aataaaacga aatgcaggga
      421 aacatactgt tataatttta acagaagata attatagaca atatgttgat ataccaaaat
      481 gggtggaaga aaagaaaaac aaaggaatca ttacgagaac aaattattct gatttattaa
      541 gattatcttt gctagcaaaa catggaggtt tgtggttaga cgccacattt tattgtacaa
      601 aaccaatacc agatgagtat tttgatatcg atatttggtc tattaggagg cctgattatg
      661 ctcacgcatc cgtagcatca ggatattttg cgggttattc attagcatgt aatcaagagt
      721 ctagatggat gtttgctata attagagact tttttttaaa ttattggaaa aataatgata
      781 tgatggtgga ttatttgatg atagattata tgattgtttt agcacagaga ataaatcatg
      841 aaatagcaca gacatttgat aaaataccta gtaacaatcc attatgtgac gaattgatta
      901 aaaatttaaa taatatatat gatgaaaaag tatggaactt attaaagaaa gatactttac
      961 tgtttaagtt gacatggaaa caatctttta aaaagcaaaa agatgataaa gttacttttt
     1021 atgggatgtt ggtagaaaac aaattattat aaatattaga ggtataaaaa gttatgaaac
     1081 acaaggtaag tgtgatagta ccaatttaca atgttgaaaa atatttaaga aaatgtgtgg
     1141 atagtttatt gagtcagaca ttaaaagaga tagaaatcgt attagttgat gatggttcgc
     1201 cagatgcttc gggggaaata gcagatgagt atcaaaaaaa atattctaat gtaaaaacaa
     1261 tacatagaga aaatgggggg ttaggaccgg caagaaatac aggaattgag aatgcgacag
     1321 gagaatatgt ggcttttctt gattcagatg attgggtaga aagtgatatg tatgaaaaat
     1381 tatacttagt tgcgtccaaa gaaaatgctg atatagtagt tagtggacat tgtgattttg
     1441 cgaatgacaa agctatggtg aaaaagcaac atcctttgag tgggaatgta tatgatacgc
     1501 ctaatagtat taaaaatgta cgaaaaaatt tatttggaca tagtccaaaa gatcaagaag
     1561 tagaggcctt cccgatgtca gtatgtatgt ctttgtataa taaaaatctg ctagataaat
     1621 atagactaag atttgaaaaa attttatctg aagatacaat ttttaacttg aatgcatatg
     1681 atgtcgcaaa taaaattgct tttacagaat atacagatta ttgttataga aaagaagaac
     1741 aagagtctat tacaaaaagt ttatctccaa ataagcagac ggaatttaaa gaatttttaa
     1801 taactctaaa agctatagca tcgaaagaag atttagaatg tcaaatcaga gcaaaacgta
     1861 tggcaattga ttattgtagg ctttatgtgg gcttagttga tgatgcaaat ttgggaatta
     1921 aagagaaaag aaagtatata aaaaaattta ttgaagatga gggattaatt caatgttgga
     1981 agggatatcc tacaaatacg ctaccatttc aacaaaaatt ttttcat
//
LOCUS       DAYYOA010000212         2003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000212 DAYYOA010000000
VERSION     DAYYOA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.219"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHBKA_002852"
     CDS             <1..378
                     /locus_tag="ACHBKA_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM9446469.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHBKA_002853"
     CDS             426..1826
                     /locus_tag="ACHBKA_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9446470.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAYYOA010000213         1995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000213 DAYYOA010000000
VERSION     DAYYOA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.220"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..503
                     /locus_tag="ACHBKA_002854"
                     /pseudo
     CDS             1..503
                     /locus_tag="ACHBKA_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            658..1233
                     /locus_tag="ACHBKA_002855"
     CDS             658..1233
                     /locus_tag="ACHBKA_002855"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9446471.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1276..1473)
                     /locus_tag="ACHBKA_002856"
     CDS             complement(1276..1473)
                     /locus_tag="ACHBKA_002856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446472.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1439..1642
                     /locus_tag="ACHBKA_002857"
                     /pseudo
     CDS             1439..1642
                     /locus_tag="ACHBKA_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1815..>1995)
                     /locus_tag="ACHBKA_002858"
     CDS             complement(1815..>1995)
                     /locus_tag="ACHBKA_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446473.1"
                     /translation="KNLKTGAIITEKEFKNLVTEEESRFYNLSGNICPGFMDQIIENE
                     SIESIENYVPVDLPK"
BASE COUNT          701 a          309 c          369 g          616 t
ORIGIN      
        1 ctaaagactc tagcgaacaa aggcgatttg aatggattat tccgtgtcca tttggaaaaa
       61 gctgtcaata cgcttctcaa aacggagtta acggctttcc tcgattacga aaagtacgat
      121 cgcattggtt ttaacacggg taattctcgt aacggctcct atgaccgtac ggtcaagacc
      181 gagtatgggg aacttcatct ccagattccg cgcgaccgca atggcgagtt caagcaacag
      241 actgttcctg cttatagacg gacgaatgac acattagagg agaccgtcat tcacctcttc
      301 cgaaaaggta ttaccatgtc ggaaatcgca gacttgattg agaaaatgta tgggcatcac
      361 tacacgcccc aaaccatgtc caacatgact aaagtgctga ctgaagaagt aaatgcattt
      421 aaagttagaa ctctaaatga taagtatgtc gctattttca tgattccttc agtttttcag
      481 atgatatacc actcaaaaaa caatatgaga gatgttcagg aataccatct ctaatggctt
      541 ataaattggt tttagggata gtagatgtta tagacatatc aaggaataaa cgagcagctg
      601 cataaaagca gatggaatta aggaataatt gaattgtaaa agaagggaat aaatttaatg
      661 gcacaattat tttacagata tggaacaatg aatagtggaa agagtattga gctactgaaa
      721 gtggctcata attatgaaga acaagggaag agagttatca taatgacaag cgcaatagat
      781 actcgatcag gatttggaaa agttgagagt cgtataggtg tttcgaaacc agctattgca
      841 attacttctg aaatggatgt gtacggagaa ttagtggaga ttattgatca gtacggaaaa
      901 gttgattgta ttttggtcga tgagtctcaa tttttaagta gagagaatat tattgatcta
      961 tcacttatcg taatgaatga ggaagtacca gtaatttgct atggattgaa gaatgatttt
     1021 caaaataatc tcttcaaggg atccgaagca ttattgacat ttgctgataa aattgaggaa
     1081 attaaaacta tttgccattt ttgcaataaa aaggctgtaa tgaatgctcg tattgttgat
     1141 ggtattccag tttattcagg tgaacagatt gtagtaggag gtaatgaaga ttacctttcg
     1201 gtatgtcgca accactacta caacgaagat tgaaataaaa aacgtgtaat caaataattg
     1261 attacacgtt ttttattatt ctgttaaagg ttgaggatca gaaattttta ggtatctata
     1321 atcctcacct aaaaatccgt taaaacgaag taaaacatca tgaccattta aatcagttgg
     1381 aactaaaatt ggaattaatt tatcaacagt agttttgttg taaagttcaa aagtgttctc
     1441 cattccttct ggttctacaa tcgaatcttt caataaacaa atcaagaaat acagccgcag
     1501 aaaagagcag tttcaaaatg aagaatcact agagcgtttc ctagtatcca tctttgatac
     1561 atacaatcaa aaattcttaa atagaagcca taaaggcttc caacaagtga cggatacatt
     1621 agcttcaatg tttactgagt aacccattat tttgcagaag gacgagttat ttacacaaaa
     1681 ttattgacgc tccctgtttg gagtggtaaa aagattgaga aattgtttca ttagctatta
     1741 cgattattat tgtaatatat ttttattgaa aatcaaaatt aaaataattc actgtaaaaa
     1801 caggattatt ttttctattt tggtaagtca acaggaacat agttttcaat tgattcaatt
     1861 gactcatttt caataatctg atccataaat ccagggcaaa tattaccaga caaattataa
     1921 aacctgcttt cttcttcagt cactaaattt ttaaattctt tttcagttat aatagctcca
     1981 gttttcaaat tttta
//
LOCUS       DAYYOA010000214         1993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000214 DAYYOA010000000
VERSION     DAYYOA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.221"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACHBKA_002859"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACHBKA_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9446474.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACHBKA_002860"
     CDS             852..1040
                     /locus_tag="ACHBKA_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9446475.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACHBKA_002861"
     CDS             1053..1610
                     /locus_tag="ACHBKA_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9446476.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACHBKA_002862"
     CDS             1642..1884
                     /locus_tag="ACHBKA_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9446477.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DAYYOA010000215         1980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000215 DAYYOA010000000
VERSION     DAYYOA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.222"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1736
                     /locus_tag="ACHBKA_002863"
     CDS             <1..1736
                     /locus_tag="ACHBKA_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFM9446478.1"
                     /translation="NSIRLSDYEFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEED
                     DEALLGSRIDMSDIFKHISVVYVDALRDVLREMKNNRNPVKRIMETIESKISSDNVES
                     LKTIIQQLNETITSVPEIRKIGENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFL
                     SMKPEQNIDLDLLGLGHLNMIYLALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKT
                     LFEGLNLQKNYTQILMTTHSVHLAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEK
                     LKKNNLNLINAVERYLDVKRNGLLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGI
                     GLINVGSTSFEYIASLFDDDRIQRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEK
                     LSSLFNENNWVNCFYANTTFEIEFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQ
                     SEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIY
                     KKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIEN
                     YTKILYAEVSK"
     gene            1733..>1980
                     /locus_tag="ACHBKA_002864"
     CDS             1733..>1980
                     /locus_tag="ACHBKA_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM9446479.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDD"
BASE COUNT          760 a          270 c          336 g          614 t
ORIGIN      
        1 gaaattctat tcgtctatcc gactatgaat tttactacac atctaaatca aatctaaatt
       61 tctgtatcaa tgaaaattat gataaattgg taggaagact caatgacaat atagctagta
      121 atcctgaaga agacgatgaa gctctgttgg gctcaagaat tgacatgtca gatattttca
      181 agcatatttc ggttgtttat gttgacgctc ttcgagatgt tcttagagaa atgaaaaaca
      241 atcgaaaccc agtcaaaaga atcatggaaa caattgaatc aaaaatatca tcagataatg
      301 ttgagtcatt aaaaacaatt atacagcaat taaatgagac tattaccagt gttcctgaaa
      361 ttaggaaaat tggcgaaaat attaatcggc aactaaatag cataattggt tcagtgtatt
      421 ctcctaatct tcttctttcc tcaactatgt cagatgatat gggttcttta gcaaaatttc
      481 tttcaatgaa gccagaacaa aatatcgact tagacttact agggttagga catctaaata
      541 tgatttattt agctttgaaa atagtcgaat ttgaagcgtg tcgttcaaga gaactactta
      601 atattatgtt gattgaagaa ccagaagctc atattcatca tcatattcaa aaaactctgt
      661 tcgaaggatt aaatcttcaa aaaaactata ctcagattct catgaccaca cattctgtac
      721 atttagctga agtgtctgaa atatctcgga tgaatgtatt aaaatcgttt aatggcaaat
      781 ctatcgttat gaatccagca aataatctga actcatttgc taaagaaaag ttaaaaaaga
      841 acaatctaaa tctaataaac gcagtagaga gatatttaga tgttaaaaga aatggattgc
      901 ttttttctaa aggtgttatc ttagttgaag gagatgctga agaaatactt attcctcaaa
      961 tagtaaaaaa attatttggt ataaacttag acgaacttgg aataggtttg attaatgttg
     1021 gtagtacttc gtttgaatat atcgctagct tgtttgacga tgatagaata cagagaaagt
     1081 gtgctattgt aactgattta gatgaacagg caatcccatc agaacatcga ttgtataaaa
     1141 gcactgcaca agagaaaggt caagcaagaa aagaaaaatt atcctccctt tttaatgaaa
     1201 acaattgggt taattgtttt tatgctaata caacatttga aattgagttt ttagatgtga
     1261 ataaagaaaa tattctaact tatgtcagtc caattataga tgacgcattc aaagataaaa
     1321 aaacaataac tgcttataaa gaagaaatac aaagtgaaca atatttagaa agaaatttag
     1381 cgatgttaaa actagccaat catgttggta aggggtggtt tgcaattgag ttaggtgaaa
     1441 aaattgactt tttaatcaaa attcctaagt atattcttaa tgctattgct tttgcatgtc
     1501 aggaaatagt agatctctca atttataaaa aaattctact ccatacaatc gacttgtacg
     1561 aggaaactac agaattgtca gctttatcag ctagtttaaa aacaagtgat acagctgatt
     1621 tgaatatagc aataatttct ttattagagt actttggtaa agatgaggat gttgtgtatc
     1681 taatcaatca gatagaaaat tacacaaaaa tactttatgc tgaggtttca aaatgacaaa
     1741 tatcttcgaa aaaatcgtaa atgatttaaa tttagaacaa aaagatgttg tggaaagtga
     1801 agataacctt tatgtaaatg catgtcctgg gagtgggaaa acaagggtac ttacaaggaa
     1861 aattgcttac caagcaatta aacacgatgg atcactaaaa agaataattg ctatcacata
     1921 cactaataga gctgctgaag aaattaagga acgcctaagt ttacttagta tagatgatga
//
LOCUS       DAYYOA010000216         1962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000216 DAYYOA010000000
VERSION     DAYYOA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.223"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACHBKA_002865"
     CDS             complement(<1..272)
                     /locus_tag="ACHBKA_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446480.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ACHBKA_002866"
     CDS             complement(309..446)
                     /locus_tag="ACHBKA_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9446481.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="ACHBKA_002867"
     CDS             complement(448..894)
                     /locus_tag="ACHBKA_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446482.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="ACHBKA_002868"
     CDS             complement(891..1040)
                     /locus_tag="ACHBKA_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446483.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>1962)
                     /locus_tag="ACHBKA_002869"
     CDS             complement(1054..>1962)
                     /locus_tag="ACHBKA_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446484.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DAYYOA010000217         1928 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000217 DAYYOA010000000
VERSION     DAYYOA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.224"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1542
                     /locus_tag="ACHBKA_002870"
     CDS             76..1542
                     /locus_tag="ACHBKA_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9446485.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          591 a          330 c          396 g          611 t
ORIGIN      
        1 ctgtcttgta agtttgttaa aaattgaata tgattacatc tggataacaa atttttatca
       61 taaaggagag aacgaatgaa caacaaacaa ttacgctggt ttactgttgc gttaattgca
      121 tttaacatgg tttggggcat ggggaatgta gttaataact atgctcagca aggagtatca
      181 gttgtcacct cttggctatt gattttggcc atttatttta ttccatacgc attgatcgtt
      241 ggacaattag gatcagcctt caaggatagt aaaggaggcg tcagttcatg ggtcgaaaat
      301 acgacatcta ataaacgact ggcatactat gccgcttgga cttactgggt cgtccatatt
      361 ccttatttgg cacaaaagcc tcaagcaatt ctgattgctg gtggttgggc agtccaagga
      421 aatggaaata tcgtaaatac gatgtcagta caaatggtag cagttatttc acttattata
      481 tttcttgcct ttttatattt atcaacaaaa ggactttcta ctttgaaagt gattggcgga
      541 ctggccggaa cagcgatgtt cgtcatgtcg atcttattta ttcttttagc tgtaacggca
      601 ccgttcatca atccaacgat ggaatttgcc acaccacata tgaatcaagt gaaaacatat
      661 attccaacat ttgatttcag ttatttcaca accgtttcga tgctagtatt tgcagttgga
      721 ggggcggaaa aaatatcccc ttatgtcaac tctacaagaa atcctgcaaa ggaatttcct
      781 aaagggatga tcttcttagc agcaatggta gggatttgtg ctgtattagg ctcagtcgca
      841 atgggtatgc tgtttgctag tgataatatt cctaaagact tgatggcaaa tggggcatac
      901 agtgcttttc aaattttagg taattactat ggtatcggta actcattgat gatcatttac
      961 gcattgacaa acatgattgg acaaatctcc gcattagcct tttcaattga tgcaccacta
     1021 aaaatcttgt tagcagatgc ggatccagaa tttgtaccaa actggctacg taaacgtaat
     1081 cgtaaaggta cattggtgaa tggttatctt ctgacaggta tactagtagg aatcattatt
     1141 ttgttgccga tttttggtat caaagatatg aatgaattag taaaatggtt gacgaacttg
     1201 aactcagttg tcatgccaat gcgttaccta tgggtgttct tggcatatat gatgttgaat
     1261 aaagcttata agaaattcaa ttctgaatat aaatttgtta aaaatccaaa aatcggtttt
     1321 atctttggtg cttggtgctt tgccttcact gcgtttgcat gtatcctagg aatggtgcca
     1381 aaagtagatt atactgctga tcctaaagca tggtggttcc aattatttac gaatatcatc
     1441 acaccaatcg tcttgatctt attaggtatg atcttgccag caattgctcg tcgagacaaa
     1501 aacaaaaaaa tcactgaacc agaaacagtg gttcagcctt aaaatagaaa tacttgttac
     1561 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaatttac gaaaattgct
     1621 tctcaaattt ttgtgaagtt tcggcttgtt tttcgaagaa gctacttctg gaacaccgtg
     1681 aatcagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata
     1741 gcccctcata tttttcgttt ggctgggctt attaccgcag atgcaagctt tggaacaccg
     1801 tttaaggaag aggttgtaaa atcagcgatt tgacctgagt attagagaaa caaacggaaa
     1861 aatagctcct catattttgc cgctttttga gctaatgctg aaggtcagct gattgaacac
     1921 catttatt
//
LOCUS       DAYYOA010000218         1796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000218 DAYYOA010000000
VERSION     DAYYOA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.225"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACHBKA_002871"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACHBKA_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9446486.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAYYOA010000219         1718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000219 DAYYOA010000000
VERSION     DAYYOA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.226"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1079
                     /locus_tag="ACHBKA_002872"
     CDS             51..1079
                     /locus_tag="ACHBKA_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM9446487.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1135..1353
                     /locus_tag="ACHBKA_002873"
     CDS             1135..1353
                     /locus_tag="ACHBKA_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446488.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1424..>1718
                     /locus_tag="ACHBKA_002874"
     CDS             1424..>1718
                     /locus_tag="ACHBKA_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446489.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGD"
BASE COUNT          649 a          195 c          323 g          551 t
ORIGIN      
        1 tttggattca aatgatgaaa tattatattt aaataaggag tatgataata atgaatagca
       61 aaaaacttca gatgggtgaa attgttagag gtcctagtca aatatctatt gatgttacat
      121 ataaatgtaa ttttagatgt aaacattgtt acaatgcgag tggtggagac aattgtttta
      181 tggataatga gttgactgat gatgaactgt tagaacttac aaaagatata tgtaatttaa
      241 agccatataa tatttgtttc tgtggaggag aaactttact gagaaaaaaa atagttttat
      301 cttgtgccac tttattaaaa gaggcaggta ttcctaatat tgctattgtt actaatggat
      361 cattagtaac tcaggatgtt gcaaaagaat tattcaaagc aggtattaat cgatttcaag
      421 taagtttaga tggaaaaaat gctagtagcc atgaagcact tagattatat ccaaacagtt
      481 ttgaaaaggc aattaatgct atcaaattat ttagagaatt gtccgaagaa gtggatgttg
      541 ctttttgtcc tactagtttt aattacaatc aattacgaga agtatatcaa ctagtaaata
      601 ctcttaaagt gaagtcttta agaattcaac catttatgct tagtggtagg ggaatgcaaa
      661 atagggaaat agtgccaact gtttctcaat atagaaaact agtttctgat attaactata
      721 ttaaaagaaa tgaagattat accaagatag attggggaga tccaattgat catatcatcc
      781 gacacagaac tttattgaaa gatttttcga attttgtttc tattaggtca gacggaggat
      841 taatggcatc accttacttg ccaattcaag ttggaaatat taaatgtcac agtctttcta
      901 aatattggcg agcaggactt tataaagttt tttctttaga tttagttcaa gatatagcta
      961 ctcaagtttc ttcaatacaa gatatgagta aagaattaaa aaaaggacat attaaatggc
     1021 ttaatgaaga tatagaaatt gatattctcg atgaatacaa acatgatatt ttaatttagg
     1081 tttatcattg gaaagtttag ataaacaaag tacttttaga aaggagggaa aaaaatgatt
     1141 tctgaacctc aaatgaataa tatgtatgaa agcccaacta taatgggagc tggtgaagct
     1201 gcgccaagag cttgttcagc aattatggtg tgtgtgttat tagcatctgc aggagtaggt
     1261 atccaagcag gagcggtgta tacttatgct gtagttgcta cagtagctgt agctgcagcc
     1321 gcagtttgga aaacagtagg attagttggt taggaattaa taggagacta actgtctcct
     1381 attgattcca atgattaagc taaggaggaa ttttaagaaa ataatgaaat acaataattt
     1441 aaaaccgaaa ttacttaatg agaacataga atatataaga gaagaaaatt atggatattt
     1501 aaccatagtt cccaaaaatg gaactgaatt gaaagataac gttttcaatc caacagtaag
     1561 atatatattg gctaactgta ctggaaaaaa cagtgtaaaa aaaatagcga atggtttaaa
     1621 aaagaagttt cctagtttag aatatgaaaa attctatcaa gatgttgtag gaactattaa
     1681 atttttcaaa cactataata ttattgatat aggagatt
//
LOCUS       DAYYOA010000220         1712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000220 DAYYOA010000000
VERSION     DAYYOA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.227"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHBKA_002875"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHBKA_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM9446490.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHBKA_002876"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHBKA_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM9446491.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          504 a          296 c          273 g          639 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg tt
//
LOCUS       DAYYOA010000221         1701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000221 DAYYOA010000000
VERSION     DAYYOA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.228"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1511)
                     /locus_tag="ACHBKA_002877"
     CDS             complement(45..1511)
                     /locus_tag="ACHBKA_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM9446492.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          576 a          341 c          303 g          481 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc a
//
LOCUS       DAYYOA010000222         1676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000222 DAYYOA010000000
VERSION     DAYYOA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.229"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHBKA_002878"
     CDS             316..570
                     /locus_tag="ACHBKA_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9446493.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHBKA_002879"
     CDS             571..888
                     /locus_tag="ACHBKA_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9446494.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1522..>1676)
                     /locus_tag="ACHBKA_002880"
     CDS             complement(1522..>1676)
                     /locus_tag="ACHBKA_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM9446495.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT          588 a          263 c          317 g          508 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc
     1621 tagcccttta acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaag
//
LOCUS       DAYYOA010000223         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000223 DAYYOA010000000
VERSION     DAYYOA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.230"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHBKA_002881"
     CDS             131..505
                     /locus_tag="ACHBKA_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9446496.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHBKA_002882"
     CDS             complement(619..1248)
                     /locus_tag="ACHBKA_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9446497.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHBKA_002883"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHBKA_002883"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYOA010000224         1647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000224 DAYYOA010000000
VERSION     DAYYOA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.231"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHBKA_002884"
     tRNA            complement(20..91)
                     /locus_tag="ACHBKA_002884"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHBKA_002885"
     tRNA            complement(113..200)
                     /locus_tag="ACHBKA_002885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHBKA_002886"
     tRNA            complement(225..298)
                     /locus_tag="ACHBKA_002886"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHBKA_002887"
     tRNA            complement(312..382)
                     /locus_tag="ACHBKA_002887"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHBKA_002888"
     tRNA            complement(384..459)
                     /locus_tag="ACHBKA_002888"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACHBKA_002889"
     tRNA            complement(478..551)
                     /locus_tag="ACHBKA_002889"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACHBKA_002890"
     tRNA            complement(564..653)
                     /locus_tag="ACHBKA_002890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACHBKA_002891"
     tRNA            complement(677..750)
                     /locus_tag="ACHBKA_002891"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACHBKA_002892"
     tRNA            complement(764..837)
                     /locus_tag="ACHBKA_002892"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACHBKA_002893"
     tRNA            complement(865..937)
                     /locus_tag="ACHBKA_002893"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACHBKA_002894"
     tRNA            complement(957..1030)
                     /locus_tag="ACHBKA_002894"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACHBKA_002895"
     tRNA            complement(1040..1113)
                     /locus_tag="ACHBKA_002895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACHBKA_002896"
     tRNA            complement(1124..1209)
                     /locus_tag="ACHBKA_002896"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACHBKA_002897"
     tRNA            complement(1226..1297)
                     /locus_tag="ACHBKA_002897"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACHBKA_002898"
     tRNA            complement(1309..1381)
                     /locus_tag="ACHBKA_002898"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACHBKA_002899"
     tRNA            complement(1399..1480)
                     /locus_tag="ACHBKA_002899"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACHBKA_002900"
     tRNA            complement(1488..1560)
                     /locus_tag="ACHBKA_002900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACHBKA_002901"
     tRNA            complement(1564..1636)
                     /locus_tag="ACHBKA_002901"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAYYOA010000225         1627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000225 DAYYOA010000000
VERSION     DAYYOA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.232"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACHBKA_002902"
     CDS             144..542
                     /locus_tag="ACHBKA_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9446498.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="ACHBKA_002903"
     CDS             526..1221
                     /locus_tag="ACHBKA_002903"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM9446499.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          617 a          235 c          321 g          454 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 ttatttctca tttaaacgat gtattcccta acctctaata catccttgta actctgaagt
     1381 tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt
     1441 tcgtgtttct ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct
     1501 tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg
     1561 gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga
     1621 tagaaat
//
LOCUS       DAYYOA010000226         1590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000226 DAYYOA010000000
VERSION     DAYYOA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.233"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..550)
                     /locus_tag="ACHBKA_002904"
     CDS             complement(<1..550)
                     /locus_tag="ACHBKA_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9446500.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADL"
     gene            complement(561..1229)
                     /locus_tag="ACHBKA_002905"
     CDS             complement(561..1229)
                     /locus_tag="ACHBKA_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9446501.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1359..>1590)
                     /locus_tag="ACHBKA_002906"
     CDS             complement(1359..>1590)
                     /locus_tag="ACHBKA_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9446502.1"
                     /translation="TSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFY
                     DILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          576 a          267 c          216 g          531 t
ORIGIN      
        1 gtaagtcagc aagtttctta gtcgctttga agtccaaatt tgaagacggt tcatccaata
       61 taattaattt agagtttagc gataaactac atccaataga aattttctgc cgttcaccac
      121 ttgatagcga aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt
      181 cattgattct tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta
      241 actcactcgt tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa
      301 tattttttga tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt
      361 ccccaaccaa ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg
      421 tggatttccc actcccagaa gaaccagtaa tcaatgtaag actgcctttt tttattgaaa
      481 aattaaggtc aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa
      541 attcaaacaa ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac
      601 aggatgatat agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga
      661 gattctatcc cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt
      721 ataagaacgg gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga
      781 atgtttctca tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag
      841 aatctgatca ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa
      901 gaggataata attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc
      961 ggcaacattc gaaatacaat atataaggat gtcgcaaaaa atatagtaaa aaactctatc
     1021 ttgatagcat caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa
     1081 atacatgtct ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc
     1141 acttgaaata tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta
     1201 atcaaggtta acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg
     1261 ttatcttaag ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag
     1321 cataactgag aacaattctc aatgcaagct ttttttaatt aactcagtaa ttcaatccac
     1381 ccaggaacag aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag
     1441 ctatgcttca atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct
     1501 tcttcattaa agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa
     1561 cagactgtct ttttcgtcac aaatgatgta
//
LOCUS       DAYYOA010000227         1588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000227 DAYYOA010000000
VERSION     DAYYOA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.234"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1099
                     /locus_tag="ACHBKA_002907"
     CDS             20..1099
                     /locus_tag="ACHBKA_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9446503.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          463 a          392 c          403 g          330 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaaa ccatgataac acagtttcac gcaagggaaa
       61 acacctaacc tactcagaac gttgccaaat cgccatcctg aaaaaggaaa actattccaa
      121 ccgtcaggtt gcggatgtcc tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg
      181 cgggaccgtt actcagctta accgccagaa tcagaacggg aaggtctacg actattatca
      241 tgacgtctac gacgccgatg ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg
      301 cagacggccc ttatgggccg acacggacgc cttcattgaa tgggcggacg acatgatgct
      361 gttggaaaaa tggtccccgg acgtggtggt gggctttgcc agaaaacacg aattgttcga
      421 cccggccatt atccctggga ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa
      481 aacgaagaat atggacctgc tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg
      541 caaccgcccg aataaacggg tgctagggaa atccattgaa gagcgcccgg aggcggttgg
      601 tacccggaaa accttcggcc attgggaagt cgacacggtc gtaggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgt tggtggaacg gcagacacgc ttcgaggtca ttctgaagct
      721 ggccgggaag gatgccgaat cggtcaacca agcggtccga cagctgagag aacgggctgg
      781 tgcgtacttc ggccagctgt tcaagacggt tacctcggac aacggctcgg agttcgcggg
      841 actgcacgat gccttgcagg atgtggcaaa catctacttc agccacccct atgcttcctg
      901 ggagcgcggg acgagcgaaa accaacacaa gctgattcgc cgtttcatcc ccaagggcag
      961 cccaatcggc aacgtgagcg aaaaccaaat cttgcggatt cagcgttgga tgaacgatta
     1021 cccgcgaaag attttgggct atgcaacgcc gcatgacacg ttcgtccagg catttaggca
     1081 ggaacggttg acggcttgac acgtgggccc ttgacaacga gcctccctcg gcatggcttg
     1141 tgtctggaaa gctggccggg gccagccagt cgggagacta ccatgcctca gctcattgtc
     1201 aagggcccac tcgattagaa aaattagacg gttggtggta acgagcaacc gggtcaccat
     1261 gggtggctaa cttaaacttg aaatttacga aaataaaaaa aggcatacag acaataaact
     1321 gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct gcaaaaaata
     1381 tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact ttgcttggta
     1441 agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat tccaataaat
     1501 aggaaggatt acaaaatttg aaaccaaata agtaaaaaat actgtcactt tgaattaatt
     1561 tgaaatagta aattctcatt aaactatt
//
LOCUS       DAYYOA010000228         1573 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000228 DAYYOA010000000
VERSION     DAYYOA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.236"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAYYOA010000229         1572 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000229 DAYYOA010000000
VERSION     DAYYOA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.237"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1508)
                     /locus_tag="ACHBKA_002908"
     CDS             complement(27..1508)
                     /locus_tag="ACHBKA_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9446504.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          500 a          214 c          276 g          582 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tt
//
LOCUS       DAYYOA010000230         1551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000230 DAYYOA010000000
VERSION     DAYYOA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.238"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACHBKA_002909"
     CDS             132..344
                     /locus_tag="ACHBKA_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446505.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACHBKA_002910"
     CDS             506..1024
                     /locus_tag="ACHBKA_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM9446506.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACHBKA_002911"
     CDS             1031..1543
                     /locus_tag="ACHBKA_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446507.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DAYYOA010000231         1522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000231 DAYYOA010000000
VERSION     DAYYOA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.239"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..221
                     /locus_tag="ACHBKA_002912"
     CDS             <1..221
                     /locus_tag="ACHBKA_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9446508.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="ACHBKA_002913"
     CDS             218..1237
                     /locus_tag="ACHBKA_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9446509.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1426..1499
                     /locus_tag="ACHBKA_002914"
     tRNA            1426..1499
                     /locus_tag="ACHBKA_002914"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Ile,seq:cat)
BASE COUNT          495 a          287 c          317 g          423 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
     1501 taacgtagcc atttgaatcg tt
//
LOCUS       DAYYOA010000232         1486 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000232 DAYYOA010000000
VERSION     DAYYOA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.241"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACHBKA_002915"
     CDS             288..695
                     /locus_tag="ACHBKA_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446510.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACHBKA_002916"
     CDS             709..1110
                     /locus_tag="ACHBKA_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446511.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACHBKA_002917"
     CDS             1112..1483
                     /locus_tag="ACHBKA_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446512.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          474 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatga
//
LOCUS       DAYYOA010000233         1483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000233 DAYYOA010000000
VERSION     DAYYOA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.242"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHBKA_002918"
     CDS             complement(<1..613)
                     /locus_tag="ACHBKA_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM9446513.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..>1483
                     /locus_tag="ACHBKA_002919"
     CDS             901..>1483
                     /locus_tag="ACHBKA_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9446514.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKE"
BASE COUNT          517 a          215 c          282 g          469 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata
     1321 aatggtgaag aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat
     1381 aataatggac aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa
     1441 gaaactcctt atgatcgagt aatagatgta tatataaaag aac
//
LOCUS       DAYYOA010000234         1453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000234 DAYYOA010000000
VERSION     DAYYOA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.243"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACHBKA_002920"
     CDS             complement(<1..625)
                     /locus_tag="ACHBKA_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM9446515.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACHBKA_002921"
     CDS             complement(784..1050)
                     /locus_tag="ACHBKA_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9446516.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACHBKA_002922"
     CDS             complement(1040..1396)
                     /locus_tag="ACHBKA_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9446517.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DAYYOA010000235         1440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000235 DAYYOA010000000
VERSION     DAYYOA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.244"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACHBKA_002923"
     CDS             105..1406
                     /locus_tag="ACHBKA_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9446518.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAYYOA010000236         1420 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000236 DAYYOA010000000
VERSION     DAYYOA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.247"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1014
                     /gene="scrK"
                     /locus_tag="ACHBKA_002924"
     CDS             142..1014
                     /gene="scrK"
                     /locus_tag="ACHBKA_002924"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9446519.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            1310..>1420
                     /locus_tag="ACHBKA_002925"
     CDS             1310..>1420
                     /locus_tag="ACHBKA_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9446520.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQT"
BASE COUNT          450 a          264 c          315 g          391 t
ORIGIN      
        1 gaattttaaa aaatgataaa aattcaaaaa gaaaaaatag tagttattta cttgtcatgt
       61 tactaaataa aaaaataatt taaatatgat atatagtcat ttatttagtg atttcattct
      121 aaaataaagg gagaaatgtc tatgctatac ggtgcaattg aagcaggcgg cacaaaattt
      181 gtgtgtgcca ttggcgatga agaaatgacg atcaaggaaa gagtgagctt tccgacaacg
      241 acacctgagg aaacaatgcc tttagtcatt gacttcttca aacaatacca agcagattta
      301 gcagggattg ggattggctc atttgggcct atcgatatcc atcgcgattc cgccacttat
      361 gggtacatta cctcgacccc gaaattggca tggcaaaatt ttgatttcat cggtaccatg
      421 aaaaaagaat ttccgatccc gatttcttgg acaacagatg tcaatgcggc tgcctatggt
      481 gagtatgtct tcggcagtgg caaaggattg agcagtgtcg tttactatac gatcggtacg
      541 ggtgttggcg gcggtgcctt acaagaaggg cgctttatcg aagggtttag tcatccagaa
      601 atggggcata tgctcgtcgt ccctcatcca aaagatgact ttgctggcag ctgtccattt
      661 catggcaact gtttagaagg gatggccgct ggacccgcca tcgaaaaacg cttagggaaa
      721 aaaggacaag aggttgcaga agatgatcct tattgggaaa tcgaagcctc ctatattgct
      781 caatgtgcct acaatacaac cttgatgttt tctccagatg tgatcatttt tggcggtggt
      841 gtgatgaaac aacgccatat gctgcaaaac gtccacgacg catttgcaaa attggtcaat
      901 gggtatgtcg aaacacctga gttaagcaaa tatatcgtga ccccagcatt agaagacaac
      961 gctggaacgc tcggttgttt ggcattggct cgagaagcgg tcttacattc ataaacgaaa
     1021 aaaggctctt cgacatttag tgttggtaga gaaagataca actgaaaatt tagtcataac
     1081 cagttaggca gagtgcttaa ctggtttttg ttttacttgg aagatcaagc cttgaatgag
     1141 ataaattctt gctcgaaagt tatggaaatt tcgatagccg taggccactt gtattgtgca
     1201 atagtttttt acacgccgtt tgtataatta agcgagacaa ataaaaaagc catttatgtt
     1261 acactcaaat cgtaataccc gctaaagcat acaaggagag tgaacataaa tgacttatac
     1321 ccatcttaca ccaaacgagc ttgtaatgat agaagcatat tttcatcaag aaactccggt
     1381 tgctatcgtt gcgaagcagc ttaaacgtgg acgccaaaca
//
LOCUS       DAYYOA010000237         1417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000237 DAYYOA010000000
VERSION     DAYYOA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.248"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHBKA_002926"
     CDS             complement(150..1337)
                     /locus_tag="ACHBKA_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM9446521.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAYYOA010000238         1362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000238 DAYYOA010000000
VERSION     DAYYOA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.249"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACHBKA_002927"
     CDS             82..>1362
                     /locus_tag="ACHBKA_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM9446522.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAYYOA010000239         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000239 DAYYOA010000000
VERSION     DAYYOA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.250"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHBKA_002928"
     rRNA            <1..1355
                     /locus_tag="ACHBKA_002928"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYOA010000240         1339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000240 DAYYOA010000000
VERSION     DAYYOA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.252"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1309
                     /locus_tag="ACHBKA_002929"
     CDS             <1..1309
                     /locus_tag="ACHBKA_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFM9446523.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          569 a          102 c          226 g          442 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaa
//
LOCUS       DAYYOA010000241         1324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000241 DAYYOA010000000
VERSION     DAYYOA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.253"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHBKA_002930"
     CDS             278..820
                     /locus_tag="ACHBKA_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446524.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACHBKA_002931"
     CDS             976..>1324
                     /locus_tag="ACHBKA_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM9446525.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAYYOA010000242         1320 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000242 DAYYOA010000000
VERSION     DAYYOA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.254"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..656
                     /locus_tag="ACHBKA_002932"
     CDS             117..656
                     /locus_tag="ACHBKA_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446526.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          495 a          174 c          210 g          441 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
//
LOCUS       DAYYOA010000243         1294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000243 DAYYOA010000000
VERSION     DAYYOA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.255"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACHBKA_002933"
     CDS             <1..1244
                     /locus_tag="ACHBKA_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM9446527.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAYYOA010000244         1287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000244 DAYYOA010000000
VERSION     DAYYOA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.256"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACHBKA_002934"
     CDS             <1..848
                     /locus_tag="ACHBKA_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9446528.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DAYYOA010000245         1273 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000245 DAYYOA010000000
VERSION     DAYYOA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.257"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..>1273)
                     /locus_tag="ACHBKA_002935"
     CDS             complement(12..>1273)
                     /locus_tag="ACHBKA_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446529.1"
                     /translation="FSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRD
                     YQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTF
                     FLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLI
                     GAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFI
                     AYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAI
                     AENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVL
                     TTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSI
                     VTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT          484 a          183 c          208 g          398 t
ORIGIN      
        1 taaataaaaa tctagtgaga atgttgtact tctcttctcg gttgtttttg tttagaaaaa
       61 gtaaatcgat aaagtaaaaa taaaatcaaa gtaacgatag ataaatatct tgaagcaatt
      121 gctagttttg tgtactgata agtaataacg actttattta tacctggagg aatagttaat
      181 tcagttgtgc caaacttaga taattttgtt tgtactagtt tgccattaat agaagccact
      241 tgccctttat aacgataaat aggtgtcgtc aagacagtat ttttattatt cgaattatct
      301 aattcaattg taagtttgtt actcataaaa tgagttgttg gatttatttc ctggttacct
      361 agtaaatatt gatcattatt aatcatatca ggatgataga ttgactcttt gtttgcataa
      421 tcagtatgat tgtaattttc agctatagca acagcgtctt caggtgctat aagtgttaat
      481 gaatctttgt tagcatggat agaattgctt actcctgaga gatgcaatat tagtgcaaga
      541 aacgtaaaga ccattatttt ccatgaatta tttttagtag aaaaaatata atagatcact
      601 atactcatcg tataggctat aaataaagaa gaaaaaccat ttaaccgcca gacaaattgt
      661 agactcttaa ctggtgtatg gttaaaaagt tgccaaggaa ataaattggt tgaacaaaaa
      721 agaacaaata caccaagaaa gaaaataaat aaatcatcag cagttaattt ttttataaaa
      781 ataaaagcac caatcaatcc gagaaataag aataatccaa tagtatagtt gttatagtta
      841 ttatttaaaa tatgtgtcag catatcacta ggagccatac cacttaattc ttttcccagt
      901 gggactttca attcttgtgc tctagattgt tgaataaaag gaattaagaa tcccgcactt
      961 aaaaaaaagg tgaggacggt agcttttaaa agacttaaga gtctagcgat tttttggtcc
     1021 caaaaataaa aagaaagaaa caacgttcct ccaataaata cagaaaccat agcgacagaa
     1081 agtaaatgag tatacactac tagcgtcatg ccaatagaca accagtacca tttttggtaa
     1141 tctctaatat aaatttcgta acaccccatt agtatcaaag gcaaaaatgt taaagcgaca
     1201 gcttctccca atgaagcacg atggaatatg tcaatggcac gataggcgga aaatgtataa
     1261 ataatcgaaa ata
//
LOCUS       DAYYOA010000246         1256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000246 DAYYOA010000000
VERSION     DAYYOA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.258"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHBKA_002936"
     CDS             <1..969
                     /locus_tag="ACHBKA_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM9446530.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACHBKA_002937"
     tRNA            1159..1232
                     /locus_tag="ACHBKA_002937"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          415 a          229 c          256 g          356 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttc
//
LOCUS       DAYYOA010000247         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000247 DAYYOA010000000
VERSION     DAYYOA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.259"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..895
                     /locus_tag="ACHBKA_002938"
     CDS             <1..895
                     /locus_tag="ACHBKA_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM9446531.1"
                     /translation="PKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWLAKKNGI
                     RQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIV
                     HNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMN
                     SDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGL
                     PMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQE
                     LKSYDVSYTTEQMESIYSRDH"
     gene            928..>1215
                     /locus_tag="ACHBKA_002939"
     CDS             928..>1215
                     /locus_tag="ACHBKA_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9446532.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGA"
BASE COUNT          475 a          130 c          220 g          390 t
ORIGIN      
        1 tcctaagaaa aatattttta aatatttaat agaagtgaat aagttactaa aaaaagaatc
       61 tttttctata gttcacaatc aggtttattt tggcggggga attaatttat ggttagccaa
      121 aaagaatgga atcagacaga gaattgctca tagccatgca actgaagatg gaaaatctca
      181 aaatattgta atgaatgtat tgcgtaaatt tttaaccaaa ttgttgttac aaaatgcaac
      241 agactacctt gccgttagtc aagaagctgg tgaaagtctg tttcaaaacc atccctttga
      301 gatagttcat aatggtattg atttagagct ttatagtaaa aattcagaag ctaaggttaa
      361 taagagaaaa gaattggata tctcaatgag tacttttgtt gtgggaaaca ttggacgatt
      421 agagaaacaa aaaaatcaat cttatttgtt agagattttt ttagaaatac taaaaatgaa
      481 ttctgattct tacttgattt tagtaggtga agggtcactt agaaataaac tgaaagccaa
      541 agcagtagaa ttggatatag agaaaagagt aaaattttta ggagaaagaa acgatattcc
      601 cgagttatta tctgtgatgg atgtttttgt aatgccttct ctttatgaag gtctgccaat
      661 ggttggatta gaagcacaag cagcaaaatt aaaattagtc ttagcagata ctatttccaa
      721 agatacaaag ttaacaaata atgtggattt tgtatcatta gatgaaacac cacaagaatg
      781 ggcaaaaaaa gtaataaaag agccgtatgc taataaaata acacaagaac taaagagtta
      841 tgatgtttcg tacactactg aacaaatgga gagtatatat agtagagatc attaaaaatg
      901 ttcaagatat tttaggagtg attatttttg aaaatatgtt atttgatatt ggcatacaat
      961 gatattggca acttaaagag attggtcaat cgattgaacg aaaatgcagt ttttattatt
     1021 catatagata aaaaaactga tatagaacca tttattgaag catttaaatc atatgataat
     1081 gttgaatttt tatgtaataa aaaccgtgtt aaaatttatt ggggtggttt ttccattgta
     1141 caagcagaaa tcaatttagt aaagagagca ttacaaaatg agaaatattt aaaatatgta
     1201 ttattatctg gtgct
//
LOCUS       DAYYOA010000248         1199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000248 DAYYOA010000000
VERSION     DAYYOA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.260"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..420
                     /locus_tag="ACHBKA_002940"
     CDS             10..420
                     /locus_tag="ACHBKA_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9446533.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            434..790
                     /locus_tag="ACHBKA_002941"
     CDS             434..790
                     /locus_tag="ACHBKA_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9446534.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          429 a          177 c          227 g          366 t
ORIGIN      
        1 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
       61 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
      121 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
      181 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
      241 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
      301 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
      361 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
      421 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
      481 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
      541 cgatctaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
      601 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
      661 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
      721 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
      781 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
      841 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
      901 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
      961 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
     1021 aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga
     1081 taagataact gagttgttaa aaaagaacca cttgcgaagc ttgattctat cgaaaaaaac
     1141 ttcaaaaaag ttcttgacaa ataacaaacg ataagttatg ataaatgagt tgctgaaag
//
LOCUS       DAYYOA010000249         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000249 DAYYOA010000000
VERSION     DAYYOA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.261"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHBKA_002942"
     CDS             <1..1076
                     /locus_tag="ACHBKA_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9446535.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAYYOA010000250         1183 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000250 DAYYOA010000000
VERSION     DAYYOA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.262"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACHBKA_002943"
     CDS             <1..153
                     /locus_tag="ACHBKA_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM9446536.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            207..575
                     /locus_tag="ACHBKA_002944"
                     /pseudo
     CDS             207..575
                     /locus_tag="ACHBKA_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..944
                     /locus_tag="ACHBKA_002945"
     CDS             681..944
                     /locus_tag="ACHBKA_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9446537.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="ACHBKA_002946"
     CDS             944..>1183
                     /locus_tag="ACHBKA_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9446538.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DAYYOA010000251         1170 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000251 DAYYOA010000000
VERSION     DAYYOA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.263"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1170
                     /locus_tag="ACHBKA_002947"
     CDS             <1..>1170
                     /locus_tag="ACHBKA_002947"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM9446539.1"
                     /translation="LSDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLI
                     ATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQ
                     SKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFY
                     TPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNT
                     TTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTT
                     FLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQK
                     LLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNK"
BASE COUNT          380 a          207 c          254 g          329 t
ORIGIN      
        1 ttatccgatc gattgttgga acaagtggtt cttttagcag atgaatcctt agaggaatat
       61 gacacagtaa gcaaacaaac aatgttgtat cgtgaattac tttcagatga agaatcaaaa
      121 gaagatctga ttgccacaat cgttgatatt ttgggatatg caattgcacc agagtatctt
      181 ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc aattgaacga tctaaacaaa
      241 gcctttgtgc aattggcatc tacctacaat caatttaatg ggttatttga tgatgtggat
      301 ctccaatcaa aaaaactagg aacagatgag caacaacgaa atgtgacgat taccgaagtc
      361 atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat cggggatgcg
      421 tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg agaattttac
      481 acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca aaaggaacga
      541 cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt
      601 aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa tacaacgaca
      661 tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga aatgaatctc
      721 cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac atttgatgca
      781 gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt cttagatgat
      841 tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt tgcgtttctt
      901 cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt accacacgga
      961 gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga agatggcagt
     1021 atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat tccaacgacg
     1081 gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga tgctagtcgt
     1141 gaatttgtta aagggaaaaa tcaaaataaa
//
LOCUS       DAYYOA010000252         1155 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000252 DAYYOA010000000
VERSION     DAYYOA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.264"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1155
                     /locus_tag="ACHBKA_002948"
     CDS             <1..>1155
                     /locus_tag="ACHBKA_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9446540.1"
                     /translation="KGISFITIPIFVNITTQAEYGLINNFTSFVSIFSILVGLSLNSA
                     INNASFDYKDDINEFMSSVLSLATLSLIVFLLIGNIYFLFSNAFFDMNQLVFNLMLLQ
                     SFASFVISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGG
                     TLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINS
                     YLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVF
                     TLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSF
                     IAIGTVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKIT"
BASE COUNT          355 a          147 c          185 g          468 t
ORIGIN      
        1 taagggaatt tcattcataa ctattcctat ttttgtaaat attactactc aagcagaata
       61 tggtttgatt aataatttca cttcatttgt atcaattttt tcgattcttg ttgggctaag
      121 cctaaattcg gcaattaata atgcaagttt tgactataaa gatgacataa atgaatttat
      181 gtcatcggta ctttctctag ctacactttc gttaattgtg ttcttattaa tagggaacat
      241 atattttttg ttttcaaatg ctttttttga tatgaaccaa cttgtattta atttgatgtt
      301 attacaaagt tttgctagtt ttgtaattag ttatatccag tcttatttta cgattaactt
      361 taaccatttt aaatatttga ttttatcttt tatatctact tttttgaata ttgcaatctc
      421 tgtagctttg atgcagacat tatattttga tgataaatat gttggcagag tagtcggcgg
      481 aacactgtca tttgtgttga taggtttagt aatttattta agaataatga taaagggtgc
      541 tcattttttt gataaaaaac agtggagata cgctttaaaa atctctttgc cgctaatccc
      601 acattcctta gcaaatatag ttttagcaca attcgataga attatgatta attcttattt
      661 aggtcctgct gcggggggaa tatattccta cattagtaac ataggtatta ttctaagcgt
      721 aatatggatg tctactaata acgcctgggt tccatggttc tatggtgaga tgagtaaaaa
      781 taatgaaaaa aaagtgaaga aagttagtaa ctattattta gtagtcttta ctttgattac
      841 gatgattgta atggtagttt cgattgattt agcaaaattt atggctccga aagactatta
      901 ttctgggtta tctttagtgg ttccgataac cttaggctat ttctttcagt ttttatatag
      961 tttaccagtt aatgctgaat tttatgagaa aaaaacatcg tttattgcga ttggtacagt
     1021 agcaagtgct atagttaata tactattaaa catgattttc atacctaggt taggaataat
     1081 tgctgctgga tacacaaccg tatttacgta ttttttactt tttatttttc attataatat
     1141 cgctaaaaaa attac
//
LOCUS       DAYYOA010000253         1143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000253 DAYYOA010000000
VERSION     DAYYOA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.265"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHBKA_002949"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHBKA_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM9446541.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAYYOA010000254         1132 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000254 DAYYOA010000000
VERSION     DAYYOA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.266"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1130
                     /locus_tag="ACHBKA_002950"
                     /pseudo
     CDS             1..1130
                     /locus_tag="ACHBKA_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          487 a          149 c          190 g          306 t
ORIGIN      
        1 atggaaacaa atggtctttc tatccaagaa actgctaaaa aatttaatat cggttcaaat
       61 agaactctaa gtaaatggat agagcaatat gaagaaggcg gtgcttcttc acttgagagc
      121 caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa acattcctaa aaaacttaaa
      181 gatgagtctc ttgaagaaga agttattcgt ttaagagcag agaacgcata tttaaaaaag
      241 ttagaaacct tgattcaaga acaagatttg agcaagaaga aatcaaggtt aaaacaatcc
      301 ttcagctcaa aaaagaattt aaattgaatc tattactttc aattgcacaa ttagcgaagt
      361 caacttatta ttactgggta aaaaaattag ataagccaga taaatatagc aagataaagc
      421 aagaaattac agcgattgta aaagaatcaa gaaactctta tggttatcgt agagtcactt
      481 tagcgttaaa gatgaaagga tatacaatca atcataaaac agttagaaaa ttaatgtccc
      541 aaatgggact tacctgtcaa atccgaataa agcgatataa atcttataaa ggaacagtag
      601 gaaaaattgc caagaatgtg ttaaaacgaa atttttcagt agatacaccc aataaaaaat
      661 gggtgacaga cgtcactgaa ttcaagataa aaggaagaaa aatctatcta tctcctatac
      721 ttgatttatt taacggagaa ataattagtt atagtatatc aactagtcca acatataaac
      781 tgattgaaga aatgcttcaa caagcaatta aaaaaaaggg aactgagggt tcactgattc
      841 tacattcaga tcaaggatgg cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga
      901 ataacattat tcaaagcatg tctagaaaag gaaattgctt agataattct gtaatagaaa
      961 atttttttgg tgtgcttaaa tcagagtttt tttatcgaga aaaattccga tcaattgaga
     1021 tatttcaaag taaattaaat gagtatatta ggtggtataa taacaaaagg ataaaactaa
     1081 agttaaatgg actatctcca gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DAYYOA010000255         1106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000255 DAYYOA010000000
VERSION     DAYYOA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.267"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHBKA_002951"
     CDS             <1..1087
                     /locus_tag="ACHBKA_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9446542.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DAYYOA010000256         1090 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000256 DAYYOA010000000
VERSION     DAYYOA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.268"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..252)
                     /locus_tag="ACHBKA_002952"
     CDS             complement(<1..252)
                     /locus_tag="ACHBKA_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446543.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSD"
     gene            complement(254..>1090)
                     /locus_tag="ACHBKA_002953"
     CDS             complement(254..>1090)
                     /locus_tag="ACHBKA_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM9446544.1"
                     /translation="LISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFL
                     LLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEV
                     IESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGN
                     SDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLK
                     TFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKK
                     EN"
BASE COUNT          418 a          182 c          131 g          359 t
ORIGIN      
        1 atccgagaaa aattgattat ggttagttaa caagttaatt ttacgtttaa aggaagaacc
       61 gattttccgg tgattcacaa aaacaacatc attaaatagc cctaattctt ttatacgctc
      121 actatatctt ctagcgtttt caaattcaga ataaataaat aaatctcctt ttgattctcg
      181 gtaaaaattt tgataaacat ttaatatatt aatcaaatgt aaaggagtcc aagcaatgaa
      241 agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt cccaaataat
      301 accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc tccaccattt
      361 catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt
      421 tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac
      481 atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc
      541 catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt
      601 ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta
      661 taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac
      721 tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat tcatttgact
      781 ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat
      841 aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga
      901 tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca aacaacaaaa
      961 gaaataatac taaaaaagaa caaattacta cctgggcacc tctagacgaa gaaagtgtaa
     1021 tataaaatat ttgtacgata aaattcatca tataaaaaac acgaatcgtt tttgttattt
     1081 ttgaaattaa
//
LOCUS       DAYYOA010000257         1055 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000257 DAYYOA010000000
VERSION     DAYYOA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.269"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1055
                     /locus_tag="ACHBKA_002954"
     CDS             <1..>1055
                     /locus_tag="ACHBKA_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM9446545.1"
                     /translation="NHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKI
                     ASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASI
                     EDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKR
                     ILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRAN
                     INHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLF
                     FSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYG
                     FKSFENMRIRIFLINQL"
BASE COUNT          352 a          210 c          194 g          299 t
ORIGIN      
        1 acaatcacat gcctttggtt atgcgcttaa aaaagcagcg ctatacctgt aaaaactgtc
       61 gaacccattg gacggctcaa agttattttg ttcaacccag acattcaatc gcgaatcatg
      121 tcagatataa aattgcttct ttactgactg aaaaagtatc tttatctttt attgcgaaaa
      181 attgtcaggt atctttgacc accgttattc gtacgttgaa agagtttaaa agctacttac
      241 caaagcaatc taagaggatt ctccccagag tattgatggt tgatgaattt cgttcgcacg
      301 cttccataga agataaaatg agctttattt gcgcagatgg cgaaacagga caattaattg
      361 atgtcttgcc tacgcgtaaa ttgcctcgat taacaagcta tttcttaggc tgcgctaatc
      421 ctgaagaagt aaaattttta gtcacagaca tgaacgccgc ctacttccag cttaccaaac
      481 gtattctgcc aaatgcgaaa gtagtgattg atcggtttca tattatcaaa cacatgaatc
      541 aagcctttaa tgagttgcgt atccgtgaaa tgaatgaact tcgtaaagta ggacagaaaa
      601 gtcaggcaga aaaactgaaa aagaactggc gctttttgct aaaaaatcgt gcaaacatca
      661 accattatga atacaaaaca tggaaaagtt ttcgagcacc aaaataccca tttcttacgg
      721 aagcgatgat gattgatcga ttgcttggct tttctacgtc cctgaaggag gcgtatccct
      781 attttcatga attagttgaa gcctttcgag acaaagaccc tgacttattt ttctccttat
      841 tggcagaact tcccgaaacg ttagatgacg gctttcggga aaagctccaa aaccttctga
      901 cctatgaaga aggcatcacc aacgcgatga tctatcctta caccaatgga aaaatagaag
      961 cgaagaatac ccatataaag acaatgaaac gagtatccta cggatttaaa tcatttgaga
     1021 acatgagaat tagaatcttt ttgatcaatc aatta
//
LOCUS       DAYYOA010000258         1051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000258 DAYYOA010000000
VERSION     DAYYOA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.270"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHBKA_002955"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHBKA_002955"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM9446546.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAYYOA010000259         1045 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000259 DAYYOA010000000
VERSION     DAYYOA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.271"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..958
                     /locus_tag="ACHBKA_002956"
     CDS             11..958
                     /locus_tag="ACHBKA_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9446547.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          336 a          162 c          227 g          320 t
ORIGIN      
        1 aggtaaaagg atgagctgtt atcttatcac cggtggggct ggcttcattg gttctacctt
       61 agccaatcat ttaggagaag aacatgtggt aatcgtcgtc gatgacttat caatgggaaa
      121 agtagaaaat cttgatatga cgaaaaatat cacttttatt gagggagatg tcgctgagaa
      181 atccttgatg gaagagatca tgaaagctaa taaatttgat tatatctttc atttagcagc
      241 agttgctagt gtggcagatt cagttgcccg accagtagaa acacatcgtg tgaattttga
      301 aagtgtatta atgattttag aattgatccg caagtatcaa cctgatttaa aacggatcgt
      361 gtttagttct tcagcggctg tttatgggga tgaaccgact ttaccaaaaa aagaagagtc
      421 cgtgattcgc ccattgacgc cttatgcggt tgataaattc gctgcagaac aatatgtgct
      481 agattattgt catttatatg atgtacccgg tagcgcagtt cgttttttca atgtctatgg
      541 tccaaatcaa aatccgaatt ctccatacag tggtgtgatt tctattctcg tagatcgcta
      601 taagaaacaa atcgtaggag aagcaacttc atttacatta tttggtgatg gaagccagtc
      661 tagagacttt gtttatatag atgatgtaat ccaagcatta cttttagttg cgaatgagga
      721 agctgcactg gggaaacaat tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt
      781 gattcatgca atcaatgaaa ttctcggtgt agaattgaca cttgaataca aagaagagcg
      841 ttcgggtgat atccatgact ctctagcaga tattactaag atccagtcaa ttggctatca
      901 accgaaatat ggtattttaa gtggaatgac taattatttg aaaactgaaa tcaattaaaa
      961 catactaaga taagattaga gggtaaattc aggctctttg tcaataagga ctgatgagtt
     1021 ttgtaaaatc aaatctggga tagaa
//
LOCUS       DAYYOA010000260          994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000260 DAYYOA010000000
VERSION     DAYYOA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.272"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..912)
                     /locus_tag="ACHBKA_002957"
     CDS             complement(25..912)
                     /locus_tag="ACHBKA_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9446548.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          320 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaa
//
LOCUS       DAYYOA010000261          994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000261 DAYYOA010000000
VERSION     DAYYOA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.273"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACHBKA_002958"
     rRNA            complement(<1..>994)
                     /locus_tag="ACHBKA_002958"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAYYOA010000262          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000262 DAYYOA010000000
VERSION     DAYYOA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.274"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACHBKA_002959"
     CDS             <1..704
                     /locus_tag="ACHBKA_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9446549.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAYYOA010000263          985 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000263 DAYYOA010000000
VERSION     DAYYOA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.275"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..985
                     /locus_tag="ACHBKA_002960"
                     /pseudo
     CDS             352..985
                     /locus_tag="ACHBKA_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          336 a          181 c          179 g          289 t
ORIGIN      
        1 aaaatgtagt tatatattta aatttttacc agaaagaaat gaacaccttc aattatctaa
       61 atctcgaaaa tggttagatg taatcatcag gaaaaacaat cttaaacgaa ttactattca
      121 tggtcttaga cacacacatg ctagcttgct tttagcaagc tagtgcaaat attaaagatg
      181 tgcaagagcg tttaggccac tcttctattc aaatcattat ggatctatat attcatatta
      241 cagacaaaag aaaagaagaa accgtagcgc aattcgcaaa atataggctc tataatttat
      301 gggactgatg aatcaaaatt gattcttcag tcccattgtt attttaggta tatgattcgt
      361 acattgaaag agttgaaaaa ctatcttccg aagccgtcaa aaaggaccct gccaaaagta
      421 ttgatggtgg atgaatttcg ttcgcacgct tcaattgtgg ataaaatgag ctttatttgt
      481 gcggatggcg agactggaaa actcattgat attttgccta cacgaaaatt gtctcgatta
      541 acacactatt ttagtcgctg tgcaaatcct gaagcggtgg aatatttagt gacagatatg
      601 aacgccgcct acttccaact gacaaaacgt gtcttaccaa atgcgaaagt cgtcattgat
      661 tgttttcata tcatcaaaca tttgaatcag gcgttcaatg attttcgtgt acaagaaatg
      721 aaaagattac accagttagg gcaacgacgg gaagccgaga aagtgaaaaa gaactggcgc
      781 tttcttctaa aaaaccgtca aaaaatcaat cactatgatt acaaaacatg gcgcagtttt
      841 cgtgcaccta aatacccgct actcactgaa gcaatgatga ttgatcgatt gctggcattt
      901 tcaacgcctt taaaggacgc ctatactttt ttccatgagc tgaccgaagc ctttagagac
      961 aaggaatcag acttattttt tacct
//
LOCUS       DAYYOA010000264          955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000264 DAYYOA010000000
VERSION     DAYYOA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.276"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..355
                     /locus_tag="ACHBKA_002961"
     CDS             86..355
                     /locus_tag="ACHBKA_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9446550.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="ACHBKA_002962"
     CDS             348..605
                     /locus_tag="ACHBKA_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9446551.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          363 a          102 c          178 g          312 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt
      841 gtagtgtggg atgttagtta ctaaaggatg acgcttatat atatgactga atagaataag
      901 caataggttt aataatctat tttaaatttt ttgtactagt tttagttaat tagca
//
LOCUS       DAYYOA010000265          931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000265 DAYYOA010000000
VERSION     DAYYOA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.277"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACHBKA_002963"
     CDS             <1..>931
                     /locus_tag="ACHBKA_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9446552.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAYYOA010000266          931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000266 DAYYOA010000000
VERSION     DAYYOA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.278"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACHBKA_002964"
     CDS             <1..>931
                     /locus_tag="ACHBKA_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM9446553.1"
                     /translation="QFCLEFIIYPFKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLN
                     LKTYELNKIDLSLSHDFKINEVQYTQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNS
                     NVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGI
                     AKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFIS
                     YDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFD
                     AINISPIKVNNHSLFFKLAILYFTESGEKVRRRK"
BASE COUNT          348 a          125 c          152 g          306 t
ORIGIN      
        1 cagttttgtc ttgaatttat tatttatcct tttaaaatga atctctcaag agtatcaaaa
       61 ggatttacaa ttattgatga ttatactcaa agagcttacg tcagccaaat taataatatg
      121 ctgaatttaa atctcaaaac gtatgaactg aacaaaattg atttgtcact ttctcatgat
      181 tttaaaatta atgaagttca gtatactcaa gtttctgaaa tgtatcacac tatgttggaa
      241 aaaaataaag aaattgattt tgatatgatt ttactagtag cgtataaaat tttaactgta
      301 aacagtaacg ttgcacgtaa tattgctagt aatataaggg cgatttatgt ggatgaattt
      361 caagatacta gagaattaca atacaatgta atagctaaat tacttcaaaa caaccctcaa
      421 attcagtcaa tgtttgttgg agatattgac caagcaattt atggatcatt agatggtata
      481 gctaaatcag ttgaagaact agaaatgcta actagtcaca cttttcaatc aaagacgcta
      541 catggctgtt acaggtctaa tcagagatta gtagacttct actctaattt tcagagttgt
      601 ccttacgaga ttgaaagcag agggaataat aaaaacgacc gtggttttat aagttatgat
      661 tgcaaagtta ctaaggagga tttacccaac ataataaaaa atataataaa ggaaaagatt
      721 gaaagcggca ttcatgaaaa agatatttgt gtacttgctc ctcaacacta tcctctgttt
      781 tcactgggtg agtatttaaa gaaagaatta ccgtattgca attttgatgc tataaatata
      841 tcaccaatta aggtaaataa tcatagttta tttttcaagt tagcaatttt gtactttact
      901 gaatcaggtg aaaaggttcg aagaagaaag c
//
LOCUS       DAYYOA010000267          909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000267 DAYYOA010000000
VERSION     DAYYOA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.279"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..777
                     /locus_tag="ACHBKA_002965"
     CDS             <1..777
                     /locus_tag="ACHBKA_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9446554.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          314 a          139 c          173 g          283 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      721 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
      781 ttaagataaa gtaaaaatgc gaaattaagt agatgagagt gatgatgttc gaaatatcta
      841 ttatttttaa taagaataca taagctatct agtttgtaaa caatgagaga aaaaacatca
      901 atatttttt
//
LOCUS       DAYYOA010000268          909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000268 DAYYOA010000000
VERSION     DAYYOA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.280"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..>909)
                     /gene="menA"
                     /locus_tag="ACHBKA_002966"
     CDS             complement(132..>909)
                     /gene="menA"
                     /locus_tag="ACHBKA_002966"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM9446555.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          325 a          185 c          159 g          240 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaatta
//
LOCUS       DAYYOA010000269          904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000269 DAYYOA010000000
VERSION     DAYYOA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.281"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACHBKA_002967"
     CDS             265..888
                     /locus_tag="ACHBKA_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9446556.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAYYOA010000270          835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000270 DAYYOA010000000
VERSION     DAYYOA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.283"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..705
                     /locus_tag="ACHBKA_002968"
     CDS             121..705
                     /locus_tag="ACHBKA_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9446557.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          269 a          116 c          174 g          276 t
ORIGIN      
        1 tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg
       61 ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt
      121 ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac
      181 agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt
      241 aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt
      301 tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg
      361 aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt
      421 tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg
      481 atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt
      541 cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt ggatagcggt
      601 acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat
      661 ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat
      721 gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt ttttgtaagt
      781 gtctactgat aacttattct caaatgttgt aaggatttta ttcatttgtt aatat
//
LOCUS       DAYYOA010000271          804 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000271 DAYYOA010000000
VERSION     DAYYOA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.284"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..539)
                     /locus_tag="ACHBKA_002969"
     CDS             complement(<1..539)
                     /locus_tag="ACHBKA_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9446558.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVV"
     gene            complement(615..761)
                     /locus_tag="ACHBKA_002970"
     CDS             complement(615..761)
                     /locus_tag="ACHBKA_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFM9446559.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          323 a          130 c           89 g          262 t
ORIGIN      
        1 acaacaaaca tttgaatgat taaaaatatt aagacaccaa acacaaaatg atcaaatact
       61 tgatattgat gagcaacata agaaacaaat aaaccaattg taagtaaata aatcatgaat
      121 atagcagtaa gtttagaact ggactgttca acaatcctat ttatagctaa ttttctatat
      181 aaccaaataa acatcagcaa aaacaaagaa tcaagcatta tctggaatac tacataacca
      241 taccctttta tcccttcaaa tgaaaacata tacgtcataa ccatggaagt aataatagat
      301 ctgaaatcat caataagcat acataatagt aatttattta aaagcactat attagttttc
      361 ttcttttcct ttaatataaa ataacttccg attataggta tagctgcgga aacatcagta
      421 aataattcaa caaaaaaagt gagtggtatc aataaacata caaaaagata ttttcttttg
      481 ttaagatcag tattagtcat ataaaataag agaaaaaaga aaaagtttcc cacgaacatt
      541 cttaatagat aagcttttgt atcgatagta atcaagagat cccctcctga ttttaggata
      601 tcataaaaaa aattttagtt acacttttta aataatgaaa aaacacattc cactaaattt
      661 gatgccggtt ttccttgagg ttttgttcca gcacctcctt taattttttt aagctcttga
      721 tctgaacatt gtttagcatt tagtctattt ttttcttcca ttacgattcc ccctacaaac
      781 tataaatact tttatgtcac tgat
//
LOCUS       DAYYOA010000272          781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000272 DAYYOA010000000
VERSION     DAYYOA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.286"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..705
                     /locus_tag="ACHBKA_002971"
     CDS             <1..705
                     /locus_tag="ACHBKA_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446560.1"
                     /translation="VFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALSGAI
                     LVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGARLSF
                     YLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIEVGC
                     IVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMIFLL
                     VPMIVSWVDNQQRISE"
BASE COUNT          256 a           78 c          142 g          305 t
ORIGIN      
        1 gtttttttcc tttattacgt attttttgaa aataaaaata caaaaaaaca taagataaaa
       61 attatcgtat ctttaatttt aattttcatg ggattgaaga gaattgaact gttagcttta
      121 agtggtgcaa ttctagtgta ttatgtttta ttaaagtggg gaaaaaagat tcaaatccgt
      181 gcattagtgt gtactatttg tgcaactgtt atttcgttag tgtttattta cattatagat
      241 acaggaattt tggcattgct tatgtcaaat ttgggcatta cagatgacgg tgcgaggctt
      301 tcattttatc tatattcatc taaattttat gaacttggtt taggatatgt aggaacagga
      361 tggacatggt tttcaaaata cttttttaat ctttatttat cgcactttag gttaaatggt
      421 ggaagaattg cagcaagcct acacagtgat atattaacta tttatataga agttggatgt
      481 atagttttta ttatatggat gttttggtta ttttttgtga aaccgataag actaactaaa
      541 aaatttggat tgaaaacagg ggagtgtgta ttattattaa ctatatatat gtttatatta
      601 tatttgacgg ataatacgct cacttatcca gacactcaaa tgatttttct cttagtacct
      661 atgatagttt cgtgggttga taatcaacaa cgaatatcgg aatagatagc taaagtatat
      721 tttaattatg aatttataat tagttgtaaa agttaatatt taacggacag gaagtagggt
      781 g
//
LOCUS       DAYYOA010000273          770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000273 DAYYOA010000000
VERSION     DAYYOA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.287"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>770)
                     /locus_tag="ACHBKA_002972"
     CDS             complement(109..>770)
                     /locus_tag="ACHBKA_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM9446561.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          213 a          177 c          148 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc
//
LOCUS       DAYYOA010000274          734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000274 DAYYOA010000000
VERSION     DAYYOA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.288"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>734)
                     /locus_tag="ACHBKA_002973"
     CDS             complement(37..>734)
                     /locus_tag="ACHBKA_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446562.1"
                     /translation="LHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLI
                     RRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDK
                     FFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAG
                     KPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQ
                     IDDFPEEIIDRIE"
BASE COUNT          229 a          129 c          111 g          265 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agat
//
LOCUS       DAYYOA010000275          733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000275 DAYYOA010000000
VERSION     DAYYOA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.289"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACHBKA_002974"
     rRNA            <1..>733
                     /locus_tag="ACHBKA_002974"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAYYOA010000276          714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000276 DAYYOA010000000
VERSION     DAYYOA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.290"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACHBKA_002975"
     CDS             complement(<1..448)
                     /locus_tag="ACHBKA_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9446563.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          124 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aacc
//
LOCUS       DAYYOA010000277          701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000277 DAYYOA010000000
VERSION     DAYYOA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.291"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..172
                     /locus_tag="ACHBKA_002976"
     CDS             <1..172
                     /locus_tag="ACHBKA_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9446564.1"
                     /translation="NNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLY
                     HANEVLKIAEEK"
     gene            173..>701
                     /locus_tag="ACHBKA_002977"
     CDS             173..>701
                     /locus_tag="ACHBKA_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9446565.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTP"
BASE COUNT          276 a           84 c          127 g          214 t
ORIGIN      
        1 aaataataaa ataagttttg aagaaaacat tgttaaagaa atgcctgaat ctgctaatga
       61 tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca aagaaaactg
      121 gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat aaatggaaat
      181 tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa atttgaaata
      241 tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct atcaacaaaa
      301 acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt tagtagaatt
      361 gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat tagctacaga
      421 tactgtaggg aaaattgaag tgatacgtga ttcattctta caactagaaa gagaagataa
      481 tatatttgat ttaattatag atttagattt gacttcacct ttacgtgaag tttctaatct
      541 tgaagaatta atagatgaac atctaaaaaa tttagataaa gacgtctact tttcagttgt
      601 ttctgcaaga cgtaatccct attttaatat ggtaaaaaga attgaaaatg aagttactat
      661 agttaataaa tcaagataca catctaggca acagacacct g
//
LOCUS       DAYYOA010000278          674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000278 DAYYOA010000000
VERSION     DAYYOA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.292"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>674
                     /locus_tag="ACHBKA_002978"
     CDS             <1..>674
                     /locus_tag="ACHBKA_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM9446566.1"
                     /translation="EILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFN
                     SGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG
                     ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPL
                     KRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVT
                     DGLSGI"
BASE COUNT          232 a           97 c          126 g          219 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tatt
//
LOCUS       DAYYOA010000279          642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000279 DAYYOA010000000
VERSION     DAYYOA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.295"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..281
                     /locus_tag="ACHBKA_002979"
     CDS             78..281
                     /locus_tag="ACHBKA_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFM9446567.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            281..631
                     /locus_tag="ACHBKA_002980"
     CDS             281..631
                     /locus_tag="ACHBKA_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9446568.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          265 a           66 c          113 g          198 t
ORIGIN      
        1 gatatttaat attacaagca atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa
       61 gaaagaaaaa gaaagtattg aaacattgtg ttattctagg aatattagga acttgtctag
      121 ctggcattgg tacaggaata gacgttgacg cagctactta ctatggaaat ggtctttatt
      181 gtaacaaaga aaaatgttgg gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt
      241 ggggagataa tctttttggt agttttagtg gaggacgata atgacttcaa taaacattga
      301 tcacacaaaa aaagtcatac atgaattata caatagttta atgacacgac ctaatcagac
      361 gaaagcatta ttagatatta ctgatgtttt atttcaggta tataaaaaaa ttgatacagc
      421 aaaatatcct gaaattcttg taaataaatt agttaattat atttacatcg tgggttttga
      481 taataaaata cgctttattg ataatgatga aaaattatta atagaattag gggatatttc
      541 taaaaaagct ggaattaata gtaaatacaa agcaaacttt acagataaat cgcaatttta
      601 tagttattca gaaacaatgc ctcaacgata aaatatagta ta
//
LOCUS       DAYYOA010000280          635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000280 DAYYOA010000000
VERSION     DAYYOA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.296"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACHBKA_002981"
     CDS             complement(<1..270)
                     /locus_tag="ACHBKA_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446569.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ACHBKA_002982"
     CDS             complement(276..>635)
                     /locus_tag="ACHBKA_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9446570.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAYYOA010000281          628 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000281 DAYYOA010000000
VERSION     DAYYOA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.298"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..442
                     /locus_tag="ACHBKA_002983"
     CDS             74..442
                     /locus_tag="ACHBKA_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM9446571.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          222 a          107 c           97 g          202 t
ORIGIN      
        1 ttgttcgttt tttatacata actattgttt ttctggtaaa ataaatcaaa ccacttaatg
       61 gaggcgtttt agaatggaaa aatttcagcc gatcatcaat caaatcaaag aaaaagaaca
      121 tcaacaaaag atcaaagacc tttttgctaa gataacagaa acgtatcccc agctagacac
      181 aactgtaaaa tggaaccaac ctatgtttac tgaccatggt acctttatta ttgcatttag
      241 ttttgctaaa aatcattttt ctatcgcacc ggaaaaagca gcgattcgtg cccttgaaaa
      301 aaatatccaa gaagcaggct acgtatatac ggataacgtc atcaaagttc cttggaaaag
      361 tgtaatcaac tgggaattga ttgaacaatt gatttcattt aatattgaag ataaaaaagg
      421 tcatgataaa ttttggcgat aaagctttat acgaataaac cttttactgg cgaggaaact
      481 attcatcacc ttatacaggt tttgttcaat cctatccttt ttatcatccc tggttaaaca
      541 cttaattgac aaagtatctg ctatttttat aaggagtctt tcttgtgctg aaaaaaatca
      601 ctgattgaat ggctttgaat tttctaca
//
LOCUS       DAYYOA010000282          546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000282 DAYYOA010000000
VERSION     DAYYOA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.302"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>546
                     /locus_tag="ACHBKA_002984"
     CDS             <1..>546
                     /locus_tag="ACHBKA_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM9446572.1"
                     /translation="TKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKNLDKHYAMI
                     GIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFIPWILGQCS
                     TIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHLYDNPVGVL
                     TNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          195 a           80 c          102 g          169 t
ORIGIN      
        1 aacgaaagac cattattttg gaaggaattt tgattatgag atgtcttata atgaagtggt
       61 cactattact ccaagaaatt atcgtttcga ttttagaaag gtaaaaaatt tagataaaca
      121 ttatgcaatg ataggaatag cagctggggt atccaactat ccactctatt atgaggctac
      181 aaatgaaaaa gggttgagta tggcaggact gaattttcct ggcaatgctg attataaaga
      241 attacaagaa ggaaaagata acgttgcacc atttgagttt attccatgga tactagggca
      301 atgctcaacc atagacgaag ctaaagaatt attggctact atcaatctag tcaatatcga
      361 ttttagtgaa aaactaccct tgtcaccttt acactggtta ttagctgata aagaaaaatc
      421 gatagtaatt gaaagtatga aagatggact tcatttatat gataatcctg tcggtgtgtt
      481 gacaaataat ccgccatttg attatcaatt atttaatctg aacaattatc gctcgctatc
      541 aaatgg
//
LOCUS       DAYYOA010000283          529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000283 DAYYOA010000000
VERSION     DAYYOA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.303"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(274..465)
                     /locus_tag="ACHBKA_002985"
     CDS             complement(274..465)
                     /locus_tag="ACHBKA_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446573.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          161 a          110 c           74 g          184 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataa
//
LOCUS       DAYYOA010000284          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000284 DAYYOA010000000
VERSION     DAYYOA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.304"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHBKA_002986"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHBKA_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9446574.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAYYOA010000285          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000285 DAYYOA010000000
VERSION     DAYYOA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.306"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>514)
                     /locus_tag="ACHBKA_002987"
     CDS             complement(107..>514)
                     /locus_tag="ACHBKA_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM9446575.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DAYYOA010000286          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000286 DAYYOA010000000
VERSION     DAYYOA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.307"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHBKA_002988"
     CDS             complement(<1..132)
                     /locus_tag="ACHBKA_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9446576.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAYYOA010000287          509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000287 DAYYOA010000000
VERSION     DAYYOA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.308"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..463)
                     /gene="spxA"
                     /locus_tag="ACHBKA_002989"
     CDS             complement(65..463)
                     /gene="spxA"
                     /locus_tag="ACHBKA_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM9446577.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          146 a          105 c           88 g          170 t
ORIGIN      
        1 agatttctct ttaccaacat catttgacaa agagccagtc ttttccaagt ttcgtattat
       61 ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt acatcgcgcg
      121 gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta tcatcaatca
      181 tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat tcttgtaacg
      241 gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc gagatgattt
      301 cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg ttcaatggtt
      361 ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac gcacgagcct
      421 ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac cactcctttt
      481 cgtgataaga atcacttttt tgtaacaga
//
LOCUS       DAYYOA010000288          507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000288 DAYYOA010000000
VERSION     DAYYOA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.310"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          226 a           70 c           81 g          130 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgct ggaagatttc gaaaaacgaa atcgaaaaaa
      421 cttttaaaaa agagttgaca attacttgtc tctctgatat gatataaaag ttgctacggc
      481 agccaaaaac gaatcgggaa acactcc
//
LOCUS       DAYYOA010000289          506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000289 DAYYOA010000000
VERSION     DAYYOA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.311"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..482
                     /locus_tag="ACHBKA_002990"
     CDS             183..482
                     /locus_tag="ACHBKA_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9446578.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          197 a           57 c           96 g          156 t
ORIGIN      
        1 aaatcttaga ataaaaattt ttactgattt aatttagaat gtttttaaaa taaaaatcgt
       61 tgccataaca gtgtattgaa aggttttaac aatccagtaa tgatttaaaa aaatctagat
      121 gtagcgtata ctttgtttat agaatgtagt gggaagaaat atatttttgg gaggaaatcg
      181 tgatgtctaa aagtgaagaa atagaaaaca tggtactaaa attatatgat ctcgttttaa
      241 atccagagat caaagaaaag gaacgtatac ttttgataga cgcaaagaca ggcttagaaa
      301 aaggtcagta ttatcctaaa gtcataaata atttggaacg tagtttacgt ccgttagcaa
      361 tcaggggaga attgtctaaa ccagtatcac cgttttatat ggagatatca acaataggaa
      421 aatttgaaaa agaattaggt cgaggaatgg cttcggcacc gattactttt gggcatttat
      481 aaaaacgagt tattcatctc aatgaa
//
LOCUS       DAYYOA010000290          506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000290 DAYYOA010000000
VERSION     DAYYOA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.312"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..483)
                     /locus_tag="ACHBKA_002991"
     CDS             complement(<1..483)
                     /locus_tag="ACHBKA_002991"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9446579.1"
                     /translation="MKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLL
                     IIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIE
                     AQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKA
                     D"
BASE COUNT          167 a          100 c           53 g          186 t
ORIGIN      
        1 atctgccttt attaaaatcg aacgttctga atctcgatga gagatatcta catttgcaat
       61 ctctctcgcc cattcttttg ccgattgatt taaacttatg aaattggtct tatcactgaa
      121 agcaacttct gtaggaactt tctccgaaaa gaaacacggt aaatctgcaa attgagcttc
      181 aatacccact aacggaaccc cttcaaataa tgatggcaac attaatacgt caaatgcttg
      241 ataaaatttt tctatatcat ttctatttcc taaaaattta atatcatcat ctatgccact
      301 tttctttgca taatttttta tttcttcttc catatcacca gttccaataa ttaataatac
      361 ggaattattc ttaatagcgt gaaattcttt aaaaatttca atcaaaaaac gatgattttt
      421 agaatatgaa attcttccaa tatgtcccat tacaaacgtc tgacttgaaa ttcctaattt
      481 cattcgtaca tcatttctaa tacttt
//
LOCUS       DAYYOA010000291          504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000291 DAYYOA010000000
VERSION     DAYYOA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.313"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..190
                     /locus_tag="ACHBKA_002992"
     CDS             <1..190
                     /locus_tag="ACHBKA_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9446580.1"
                     /translation="NQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKND
                     VEDTANKAQQIVNEHTIQ"
BASE COUNT          210 a           58 c           72 g          164 t
ORIGIN      
        1 taatcaaatg ttgagtgaaa aaaataacat tcaaaatcaa ctgcaagcta ctcagcaaaa
       61 gctagaagaa gaaaagtcaa aaaatagtag tttgaataat tacattgaaa agcttcaaaa
      121 agctaaaaat gatgtagagg ataccgcaaa taaagcacaa caaattgtaa atgaacatac
      181 aatccaatag atatagtttt aatttaacaa atggtatgtt tggagataat gagatagaat
      241 ataaatgtca taatattata caatctaaac accgtttcct tttctatttt tgatgacatc
      301 agtatatagc tactcgaaag agtagtcttt ttattattct cgcttattat tgatatgaaa
      361 atttcgattt gacaaaaggt accgaataat tttgtaatat gaattattat caagaagtaa
      421 ttatgatgat aacatatgga aactagttag taataataac aggtgattaa acttttgaac
      481 gacttgtaat tctatatttt ttct
//
LOCUS       DAYYOA010000292          496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000292 DAYYOA010000000
VERSION     DAYYOA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.314"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>496
                     /locus_tag="ACHBKA_002993"
     CDS             <1..>496
                     /locus_tag="ACHBKA_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9446581.1"
                     /translation="SDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWF
                     LGYSFEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHI
                     KANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISK
                     ADPES"
BASE COUNT          165 a           90 c          106 g          135 t
ORIGIN      
        1 tccgatttag gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag
       61 tatttattcg gtatccgttc catgcgacaa accattaagg aagtcgatac caatgtcgct
      121 taccgctggt tcttaggcta ctccttcgag gagaaaatcc cccatttctc aacttttgga
      181 aagaattatg ttcgccgatt tagagaaacg acggtgtttg aggatatttt tgcctatatt
      241 ttagaacaag cagttaaagc aggttttgta acagaggata atttgtatct ggattctacc
      301 cacatcaaag ctaacgccaa taaacataag tttacgaaag aaatgaccca tgatgaagcg
      361 aaagcctatc aggatgaatt agaagatgag atcaatcgtc aacgaataga ggctggaaaa
      421 cgccctttta ctttggactt agaaaaggaa gtgaagctga aagaaagaaa aataagtaaa
      481 gctgatccgg aaagcg
//
LOCUS       DAYYOA010000293          485 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000293 DAYYOA010000000
VERSION     DAYYOA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.315"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..259)
                     /locus_tag="ACHBKA_002994"
     CDS             complement(62..259)
                     /locus_tag="ACHBKA_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9446582.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAYYOA010000294          465 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000294 DAYYOA010000000
VERSION     DAYYOA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.316"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>465
                     /locus_tag="ACHBKA_002995"
     CDS             <1..>465
                     /locus_tag="ACHBKA_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM9446583.1"
                     /translation="EGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEAT
                     QPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTK
                     YTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          190 a           68 c          109 g           98 t
ORIGIN      
        1 agaaggaaca agtattgaaa gtggaacaac agtctttgca gtgaaagacg gcgtaagctt
       61 accagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg cgaaatggcc
      121 agaggaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa
      181 actggatgat aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag tgactacaga
      241 attgaataag gaaccagacg catttgaggg aattaagaac aaagaagatt taccaaaaga
      301 tactaagtat acttggaaag aaaaagtaga tgttagcgca gctggaaata aaaaagggac
      361 tgttgtagtg acatatccag atggatcatc tgatgaagtt gaagtagatg ttacagtgac
      421 agacaatcgt tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAYYOA010000295          453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000295 DAYYOA010000000
VERSION     DAYYOA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.317"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>453
                     /locus_tag="ACHBKA_002996"
     CDS             <1..>453
                     /locus_tag="ACHBKA_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM9446584.1"
                     /translation="KLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLT
                     LDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHR
                     TIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKE"
BASE COUNT          174 a           80 c           84 g          115 t
ORIGIN      
        1 aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg
       61 gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca
      121 gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct
      181 tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc
      241 ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa
      301 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
      361 cgcaataaat tctatcgaag tttacgcact gcctctacca cgattaaagg catggaagcc
      421 attcgaggat tatataagaa aacccgaaaa gaa
//
LOCUS       DAYYOA010000296          452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000296 DAYYOA010000000
VERSION     DAYYOA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.318"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACHBKA_002997"
     CDS             <1..>452
                     /locus_tag="ACHBKA_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446585.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAYYOA010000297          439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000297 DAYYOA010000000
VERSION     DAYYOA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.320"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..>439
                     /locus_tag="ACHBKA_002998"
     CDS             124..>439
                     /locus_tag="ACHBKA_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446586.1"
                     /translation="MLSYFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSN
                     AEFENFIKLCSTLLYLILAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSY
                     GLN"
BASE COUNT          136 a           42 c           74 g          187 t
ORIGIN      
        1 atgagtgtaa aaaaaatgct atttatggta agtacaataa ttgtattaac atcccgattt
       61 tggggaatta atactagttt atctatgcga tattttatta tggcaatatg ggatttatat
      121 tttatgttaa gctatttttc ttatcgtgag aaaacatata aaaaatacgt tatcaagaat
      181 aacttttggt tgactataat gccgtttgtg atattttcta tctatactct aataatatgg
      241 ggaattggtt caaatgcaga atttgaaaat tttataaagt tatgttccac gttgctttat
      301 ttaattcttg catatggtta cgcatgtaca gcattttact tatttaaggc agaaggaatt
      361 gattttattt tttggtcagg tgttatttca tatatgagtg gttcagtttt ttatttaatt
      421 gtatcttatg gtttaaatg
//
LOCUS       DAYYOA010000298          400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000298 DAYYOA010000000
VERSION     DAYYOA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.322"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          111 a           98 c           76 g          115 t
ORIGIN      
        1 ttataaataa ctcataaaaa atattttgac tttcaaaatc acttttgcta ccagaagttg
       61 aggcgaagtg ttttaagcat agttgtaccc tagtttgacc gttaaaaata aataacgatc
      121 gctagagtgc aactatgttt gtttgcccta ccgtcagcgt cggcttggcc tctcacatcc
      181 attaccgtcg ggattatctc cgttacatac ccttacaaaa tacgccctgt aagcattgca
      241 tgaaaccacc gtgcatttgc accgtatttc cgccctagcc gttcaaatcg gtcgctacca
      301 ccattcagaa acacgtgcgg taatggaagg tttggaggaa gctatccaga ccactaacga
      361 ttgtccctat cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAYYOA010000299          378 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000299 DAYYOA010000000
VERSION     DAYYOA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.323"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..>378
                     /locus_tag="ACHBKA_002999"
     CDS             103..>378
                     /locus_tag="ACHBKA_002999"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9446587.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKID"
BASE COUNT          144 a           39 c           73 g          122 t
ORIGIN      
        1 aatgatgttt gtacttactt aaattaggta taattagtat atgttttgtg agaaatttag
       61 acttacaata agcagtgaat ttataatcaa cgtataggag acatggatga aatgaaacta
      121 attggtatag atttagacgg tactcttttg aattctgacc agagcattag tcaaaaaaat
      181 gcaagaacaa tgaaacattt accggaagac tgttttccat tcatttgttc tggaagagaa
      241 gtagaagaca ttaataatat tctcaaaaaa tctggtttaa ggataccagc agtagggtta
      301 aatggttctg ttggatttga tggagaaaaa aagatatttg agtcttcctt cgataaaaat
      361 gatgtaaaaa aaattgat
//
LOCUS       DAYYOA010000300          343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000300 DAYYOA010000000
VERSION     DAYYOA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.326"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACHBKA_003000"
     CDS             complement(<1..321)
                     /locus_tag="ACHBKA_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9446588.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           96 a           55 c           63 g          129 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tga
//
LOCUS       DAYYOA010000301          338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000301 DAYYOA010000000
VERSION     DAYYOA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.328"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..338
                     /locus_tag="ACHBKA_003001"
                     /pseudo
     CDS             1..338
                     /locus_tag="ACHBKA_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DAYYOA010000302          331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000302 DAYYOA010000000
VERSION     DAYYOA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.329"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>331
                     /locus_tag="ACHBKA_003002"
     CDS             <1..>331
                     /locus_tag="ACHBKA_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFM9446589.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAYYOA010000303          330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000303 DAYYOA010000000
VERSION     DAYYOA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.330"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..>330
                     /locus_tag="ACHBKA_003003"
     CDS             20..>330
                     /locus_tag="ACHBKA_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446590.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     T"
BASE COUNT          112 a           48 c           40 g          130 t
ORIGIN      
        1 ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata tcatttttat
       61 gttatttcta agttttgctt acgtcatccc tctattcatg tctaagggaa tctatcatag
      121 tgtcaatcag gatacttact ttcatttatc tcgaatcata ggactagata atgtatggag
      181 ttctccagta aacttcaata acttcgatca tcatggaaca atgatgaata ttttttatcc
      241 atggcttact ctttatccag cttttttatt ttataaaatg atgggaaatc ttgttttaag
      301 ttataatatc tactattttt ttatcacttt
//
LOCUS       DAYYOA010000304          327 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000304 DAYYOA010000000
VERSION     DAYYOA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.331"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..>327)
                     /locus_tag="ACHBKA_003004"
     CDS             complement(74..>327)
                     /locus_tag="ACHBKA_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9446591.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT           94 a           59 c           51 g          123 t
ORIGIN      
        1 agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactctttt tttcatgctc
       61 taaaatgata tttttacaca accgtaacgg ttgttttacg aaattggacg ggactttgcc
      121 agtttaattt ctgttttatc cgttcattat tgtagtaata gatatagcta tcgatggctg
      181 attttaattc ctcgaaacag ttataaactt tcccgtgaaa aatctcttgt tttaataagc
      241 caaaaaaatt ctccataagt gaattatcta agcagttccc tttccgagac atgctttgaa
      301 agattttgta ctccttcaat ttgtttc
//
LOCUS       DAYYOA010000305          322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000305 DAYYOA010000000
VERSION     DAYYOA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.332"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>322
                     /locus_tag="ACHBKA_003005"
     CDS             <1..>322
                     /locus_tag="ACHBKA_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9446592.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQK"
BASE COUNT          128 a           60 c           49 g           85 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa at
//
LOCUS       DAYYOA010000306          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000306 DAYYOA010000000
VERSION     DAYYOA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.333"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHBKA_003006"
     CDS             complement(<1..>321)
                     /locus_tag="ACHBKA_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9446593.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYOA010000307          308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000307 DAYYOA010000000
VERSION     DAYYOA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.335"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACHBKA_003007"
     CDS             complement(76..>308)
                     /locus_tag="ACHBKA_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9446594.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAYYOA010000308          299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000308 DAYYOA010000000
VERSION     DAYYOA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.337"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..299
                     /locus_tag="ACHBKA_003008"
                     /pseudo
     CDS             1..299
                     /locus_tag="ACHBKA_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           59 c           56 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcag
//
LOCUS       DAYYOA010000309          290 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000309 DAYYOA010000000
VERSION     DAYYOA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.338"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHBKA_003009"
     CDS             <1..156
                     /locus_tag="ACHBKA_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM9446595.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT          115 a           24 c           58 g           93 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc
//
LOCUS       DAYYOA010000310          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000310 DAYYOA010000000
VERSION     DAYYOA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.340"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHBKA_003010"
     CDS             <1..>282
                     /locus_tag="ACHBKA_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM9446596.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYOA010000311          274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000311 DAYYOA010000000
VERSION     DAYYOA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.342"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..>274
                     /locus_tag="ACHBKA_003011"
     CDS             93..>274
                     /locus_tag="ACHBKA_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9446597.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           57 c           44 g           89 t
ORIGIN      
        1 gcttctttta cctatgaaat attttcacgt gttaaagtat tcctcgttga acacaacaaa
       61 taaaactttt taacgaataa aggagatttt tcatgacatc acttaaaact ttactttttg
      121 gaacaacttt ggctgccggc gctgcattct tcatgggaac gactgctcat gcggacgaag
      181 cttataccgt tcaatcaggt gatactctat caacgatttc tcaaaaatac gttggtgata
      241 actccttgat caatgccatt gcagaatcaa attc
//
LOCUS       DAYYOA010000312          270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000312 DAYYOA010000000
VERSION     DAYYOA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.343"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACHBKA_003012"
     CDS             <1..>270
                     /locus_tag="ACHBKA_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9446598.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAYYOA010000313          261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000313 DAYYOA010000000
VERSION     DAYYOA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.345"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>261
                     /locus_tag="ACHBKA_003013"
     CDS             <1..>261
                     /locus_tag="ACHBKA_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9446599.1"
                     /translation="LTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGK
                     TALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT           98 a           45 c           49 g           69 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt c
//
LOCUS       DAYYOA010000314          255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000314 DAYYOA010000000
VERSION     DAYYOA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.denovo.347"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           46 c           39 g           91 t
ORIGIN      
        1 aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa
       61 tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct cctttcacaa
      121 gtatggtgat aaaattcgtt ccgggctacc tttttaggta gcctacttta atctttatac
      181 ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg
      241 tccggcacgt cagtc
//
LOCUS       DAYYOA010000315        11899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.081, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000315 DAYYOA010000000
VERSION     DAYYOA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.081"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..1055)
                     /locus_tag="ACHBKA_003014"
     CDS             complement(114..1055)
                     /locus_tag="ACHBKA_003014"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9446600.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1181..2302
                     /locus_tag="ACHBKA_003015"
     CDS             1181..2302
                     /locus_tag="ACHBKA_003015"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9446601.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2560..4062
                     /gene="eat(A)"
                     /locus_tag="ACHBKA_003016"
     CDS             2560..4062
                     /gene="eat(A)"
                     /locus_tag="ACHBKA_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9446602.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4129..4656
                     /locus_tag="ACHBKA_003017"
     CDS             4129..4656
                     /locus_tag="ACHBKA_003017"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9446603.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            4867..5928
                     /locus_tag="ACHBKA_003018"
     CDS             4867..5928
                     /locus_tag="ACHBKA_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9446604.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5931..6827
                     /gene="murQ"
                     /locus_tag="ACHBKA_003019"
     CDS             5931..6827
                     /gene="murQ"
                     /locus_tag="ACHBKA_003019"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9446605.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            6841..8823
                     /locus_tag="ACHBKA_003020"
     CDS             6841..8823
                     /locus_tag="ACHBKA_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9446606.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(8888..9334)
                     /gene="nrdI"
                     /locus_tag="ACHBKA_003021"
     CDS             complement(8888..9334)
                     /gene="nrdI"
                     /locus_tag="ACHBKA_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9446607.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            9545..10969
                     /locus_tag="ACHBKA_003022"
     CDS             9545..10969
                     /locus_tag="ACHBKA_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9446608.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11150..>11899)
                     /locus_tag="ACHBKA_003023"
     CDS             complement(11150..>11899)
                     /locus_tag="ACHBKA_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9446609.1"
                     /translation="LFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIIDPVEMTAQL
                     FSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLVIYSVYLYL
                     LAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSLVLMIACFI
                     NSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGNEEIMKQLT
                     HLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
BASE COUNT         3799 a         2101 c         2596 g         3403 t
ORIGIN      
        1 aacaagcaca attcttatgg catcatgaga gttgtgtttt ctttttgatc ttttattggg
       61 cttaaaaaaa gagaaccagc tgctgattca gctgattctc atcagagagc cttttattct
      121 gcgccaagga ttccttcttt tgcatggact aattgggtca aaaaggcaca atatggtgta
      181 gcaatatcat atttttgtcc ttttcgccaa atggcaccat tgatgtagtc gatttcagtc
      241 aaacgatggt ttttgatcag gtcttggtac atagatgggt aatgcaaccc gatgttgtca
      301 ggatcataag tagaagcaat atgttggtac acttcttctt gatctagtat gattccttct
      361 tttgctgcga tagaagcaaa ttcagatacg attgttcgaa ccatgctctc tgccgctttt
      421 tgcgcgccta gttcagcaat attacaatcc aaaatcgtgc ataaaccatt caatgttcca
      481 tttacacaag cttttcgcca aatggaataa cgaacgttat ggctatagat cggatttaaa
      541 tttgcttctt gaaagacttc tacaactttt tttgtaaatg cttcgccttc tggttccaag
      601 ttctctagtt cgatttctcc atcacctaac aatgtgactt ttccgggacc agctagacca
      661 gctgtccaca tcgtgatccc aaataagata tttttctttg gcacatattt ttccagtaca
      721 tcttcatgac ctaatccatt tagtaaacac aaaacataag tattgtcagt aataatggat
      781 tggatcgaac aaaacatatc atctaattga ttggcttttg ttaatgctac gatcaagtcc
      841 acgtgttcgt tgctttcgat gatttcttct ggtgaataga ttggcagttt tgcgaccact
      901 tctttaccgt taaagtcagc aatcaaccca tttgtacgaa tggcttcaat gtgagcaggc
      961 caacgatcga tcaagaggac ctcatttccg ctttgatgta gcatcaatcc gattcggctg
     1021 cccattgctc cggccccagc tattgcgatt ttcatgtttt ttctctcctt tattcaatga
     1081 gttccctttt agtatataga aaaatggaag aaaatagcat tgttttgttt gaaaaaaatg
     1141 aaaaaaagtg tcttacttaa gaagaaagtg agggatacag atggatattt tggcggcaat
     1201 tgattcattt aaaggaagtg cttcttcatt tgaactaaat caggcagttc taaatggctg
     1261 ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca gatggcggag aaaatactgc
     1321 agaagcaatc tattacgcgc tcaggggaac atggcgtacc attgatggga tggatctctt
     1381 attccgtcag cgaaaaatca gctatttatt gacaacttgg caaggaaaaa acaccgcagt
     1441 catcgaatct gcacaagcat tggggttaga tctacttgat aaaccaacgg atcaaactat
     1501 ccgacttgct tccagttatg ccttaggaga acttattaaa gatgcactgc ttcaagaagt
     1561 agaacaaatt attgtcactt taggaggaag cgggtgtagc gatggtggtc taggattact
     1621 ccaatccttg ggtgcttctt tagaagggta cacagaagga aatcctttat tatctactaa
     1681 aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa gtaaaattga ttatcgctgc
     1741 agatgttacc aatccatata caggtcaaca aggagctgct tggcaatttg gaagacaaaa
     1801 aggaggaata gatacgacgc tagctttctt agataaacaa gcaggaaaga ttgctcagca
     1861 aataaaagat ttctgggagg tggatttaga tgatattcca ggaagtggag cagcaggcgg
     1921 tattggtgga gccctttttt tgttgggagc gaagatggtt tctggtttcg atttagtcag
     1981 ccaaatcact ggattggaaa aaaagatcga acaagcagat gtcatcatta caggagaagg
     2041 gcggatcgat cagcaaacga tccacggaaa agttccatac ggtgtagcag tgcttgcaaa
     2101 aaaatatcga aaaccaataa tcgctatttg cggaagtaga gaccaagagc tagaaggact
     2161 ttctgatttt ctcccaattg tcttaagtat tcatttgggt ccaataggtt tagaagaagc
     2221 aatggaacac aggcaaacat tagaaaaaac aaaaatctta ggacagacac ttagcagatt
     2281 tttcaccatc ctcccgaagt agttgacaaa atcattttct ttatgctatc gtaatttcaa
     2341 atataggaca aagtgaggga tccgtgcatt gaaaaattaa gtcgattgat gaaagctgca
     2401 ttcagactgt accttgtgga gacaaggaag cagtgtgcag attttcggga ttggcttggt
     2461 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt tagccagttc
     2521 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
     2581 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
     2641 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
     2701 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
     2761 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
     2821 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
     2881 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
     2941 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
     3001 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
     3061 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
     3121 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
     3181 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
     3241 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
     3301 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
     3361 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
     3421 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     3481 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     3541 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     3601 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     3661 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     3721 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     3781 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     3841 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     3901 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     3961 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     4021 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     4081 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     4141 acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg ggcagatgtg
     4201 ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt agtagatttg
     4261 cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga tacagcacaa
     4321 gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt agaaatggat
     4381 tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg
     4441 ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg gatcgttcct
     4501 ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg ccataatgcc
     4561 ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt ctttacattc
     4621 atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt tatttaaata
     4681 aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg acaattcttt
     4741 tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa agtattgaaa
     4801 ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca gaaaaagagg
     4861 gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga gaagaaacag
     4921 tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat gcatatacca
     4981 gaagatgatg tttcggcata tcaaaagcga ttgatcgaac taggaagcga agcgaaaaag
     5041 aataaactag aattgatggt cgatatttca ggagaggcat tggaacgagc gggtttttca
     5101 ttagaagatc taacgccttt gaaagcaatt ggtgtaacag gtttaagaat ggattatcat
     5161 atttccaatc agcagattgc cgactggtca catcaattga aaatcagttt gaatgctagc
     5221 acgatcacgc ctaaagatat cgatgaattg aaagaagcag aagctgattt ctcacagatt
     5281 gaagcttggc ataattatta tccacgtccc gaaacagggc tggacaaaga atggtatcag
     5341 aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc aaggttttgt accaggagat
     5401 acagagttga gaggcccact atatcaagga ttgccaactt tggaagaaca tcgagggatc
     5461 catcctcttg caggcgctct agatctatta gcaagtaaca cggatattgt ttacatcgga
     5521 gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt ctttccaaaa agaacagaca
     5581 gtcttgctcc atacggaacc tgtagatgaa gaattttacg aatatatatt agggaagcat
     5641 acgaatcggc aagacgatgc aagagatgtc atacgaagcg tagatgcacg ttttcgagag
     5701 attccgccta tcccagcaag aaatacagct acgcgtatga aagggtctat tactttagat
     5761 aacgaaaagt atttgcgcta tatgggtgag atccaattaa ccaaatatga tttgccagcg
     5821 gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag agctaccatt gatcaaccaa
     5881 atcaaagctg gtatgaacta tcaatttata agaaaagaag gaagataaaa atgagcaaaa
     5941 tcaatttaga gaacctaaca acggaacgca gaaaccaaga aacttttgga ttagatgaga
     6001 tgagtatttc tcaagccgtt caactaatga acaaagaaga tcagaaagtg gccgctgcgg
     6061 ttgaaaaaga actgacaagt atcgaaccag tgattgctaa cacgattgag tcctttaaaa
     6121 aaggtggacg tctgatttat atgggagccg gaacaagtgg aagactaggt gtgttagatg
     6181 ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat ggtcataggt ctaattgctg
     6241 gtggagaaga agcaatgact gtagctgtcg aaggggccga agattcttat gagctagggc
     6301 aagaagatct aatcaagctg aatttgacaa aaaatgatat agttattggt atcgcagcta
     6361 gcggtcgtac accatatgtg atcggtggat tagattacgc aaaatcgatc ggtgctgtca
     6421 caggaacgat ttcttgcaat aaacaagccg aaatcagtaa acacgcggat ttcccaatcg
     6481 aagtcgatgc aggtccagaa tttttgacag gctctactcg attgaagtca ggaacagcac
     6541 aaaaacttat cttgaatatg atttctacta tcagtatgat tggtataggt aaagtattca
     6601 ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact aatcgaacgc tcaaaacgaa
     6661 tcatcatgca agcgacagaa gctgattacg aaacagctga aaagtacttt gaagaagcag
     6721 atcaaaatgt taagctggct atcgtcatga ttttgacaaa tagtgataaa gaggaagctc
     6781 aagcaaaatt agctgaagca aacggatttg taaaagggac cttatgaggg ggaaaagacc
     6841 atggcaaaaa acgaactgac tttggaagaa ctggcaaaac aaatttataa aggggcaggc
     6901 ggcatgggca atgtagaaag cattgtccac tgcatgactc gtgtgcgtat gaagatcatc
     6961 aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc caggagtgct tggtgtagta
     7021 gaagatgaac agcttcaagt catcatcgga cctgggaaag tgaacaaagt agcacagcaa
     7081 atggttgatt tggcaggcgt gaaattagga gaaaaacttt cttctgtaag tacagctaca
     7141 gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac gggcacaaga aatgaaagcg
     7201 gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg tattaaaaga catttctaat
     7261 atctttgtgc caatgattcc tgcatttgtc ggtactggga tcgtggcagg gattgccgca
     7321 gttttagcaa acttagtcac agccggaaca cttgatgcag caacatggca acaatatatc
     7381 gatgtgatga acatcttgaa aaacgggatg tttagctacc tagttatcta tacaggtatc
     7441 aatgctgcac aagtttttgg tgcaacacca actcttggtg gagttatcgg ggccgttgtg
     7501 atgctgacag ggatgaatcc ggaagcacca ctgaaaaact tatttactgg tgaagctctt
     7561 gcagctggac aaggtgggat cctaggtgtc atcttcgctg tttggctatt gtcaatcgtt
     7621 gagaaaaaac tgcataaagt agtacctgat gcggtagata tcatcgttac accaactctt
     7681 tcattgattg ccatcggtct gattgaaatt ttcttcatca tgcctttagc aggtttcatt
     7741 tctgatggca tggtcggtgg gatcaactgg gtacttggcg ttggtggagc atttgccggc
     7801 ttcgttctag gcacattatt cttgccgatg gttatgttcg gactgcatca aattttgact
     7861 ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc tattgcttcc aattcttgcg
     7921 atggctggtg caggacaagt gggtgcagca ttggcattat ggattcgctg caaaaaagat
     7981 aaagaattaa cagaaatgat caaaggagct ttaccagtcg ggatcctagg aatcggcgaa
     8041 ccattgattt acggagtaac attacctcta ggacgcccat ttattactgc ttgtatcggt
     8101 ggaggtatcg gcggggcagt gatcggaatg atcggaaatg tcggggctat cgctattgga
     8161 ccttcaggag ccgctttgat cccattgatc tctgacggta aatggttagg ctacatcctt
     8221 ggattattgg cagcttatgc tggtggcttc gttgctacat tcttcttcgg tattcctaaa
     8281 gaccgtttag aaaaagaaga attagcgact gaaacagcca cagtaaatga agtgcctgaa
     8341 aaagtagtac ctcaaacttc agctgaaagt gtgactatcc tctcatcagt tgctgacgga
     8401 acagttgttt ctttagataa agcaagtgat ccagtttttg cacaaaagat gatgggtgaa
     8461 ggctactttg tagaaccaga aaacggacat atctattctc cagttactgg ggtagtcagc
     8521 tctgtcttcc caacgaaaca cgctattggg atcacaacgc caaatggttt agaaatcttg
     8581 cttcatatgg gaatcaacac agttgactta ggcggtacgc catttgatgt gaaagtaaca
     8641 gaagggcagc aagtgacacc tgatacgctt gtagcagaag ctgacttaga agcgatcaaa
     8701 gcagcaggca aagaaacatc catgatcgtc ttggtaacca atatggatcg tgtaaaaaac
     8761 tttgtcttgg aaaaaacagg taaagcaaaa gcaaaagcac ctgtgatgga tgtcaaagct
     8821 taatatgata gaaacaatag aaacgaaaga agaaactgaa atctattttt cagcttcttc
     8881 ttttttatta tttattgttt tctgctaaaa tctgataaat tcgtgtcaca tcagatggtg
     8941 ttccccgtaa ttcataatct gccaagaaag gaaagccaaa tgtttgtgca tattgtttgg
     9001 ctgttagaca atactgatta ttgaagtttt tatttccgct accgactaca ccaagacaaa
     9061 gtttatgatt gtcttcatgt tccagatatt cacgcatcgt ttcggtcaag atttctgtat
     9121 caccgttatc taaaccattt ccgccgtcta aataagtagg aacgaaagta aaaaatggtt
     9181 ctgtttcttt cgtaaaatca ctattttcat ggatttcttt taaagtaact tcaggattga
     9241 caggatcttc tgcatgcatt ttttcagcat attctgctag atgtttggca aaggcacgcg
     9301 tatttcctga aatcgatata tataaaatgt tcatggatca tcctccaatt ctttcctttt
     9361 agtatagcat cttttttaag ttttttggtg gtgtaatttg gaaagggact aggcgctttg
     9421 cttgtataat gagaaaaaag ccggtagaat tatatttcta ttaaagtttt tattaattaa
     9481 gtaaaaataa aaaaggtttg ttatactttt tgataaaagc ggttttattt tggaggatgc
     9541 ttacatggat acagagcaac agaaaaaagg aattggttta attccacttg ctgccttagt
     9601 aattggttca gcgattggag gtggcgtatt tggtattatg acggacatct ccggatcggc
     9661 tggcggccca gctattttca gttgggtact cgtaggaaca gcaatgttga tgctctcctt
     9721 gtcgatcaat aatatcaata aaaaacgtcc agatttaaaa ggcgggatct ttagttacgc
     9781 agaagaaggt tttggccgtt tcgctggttt tatcagcggt tggggttact ggctcacatg
     9841 ctggttaggc aacgtagctt ttgcaacatt gttgatgagc gcattaggat atttcttccc
     9901 agtatttgaa ggtgggcaaa atctggcatc gatcgtgatg agtacagtct ttttatgggg
     9961 atatgcatgg ctagtgaatc ggggagtcga aaatgctagt ttcgtcaatg ctatcgtcac
    10021 attctgtaaa ttagtgccat tatttttatt tattgtagta gctatcagca cgttttcgat
    10081 ccaacaattc attgataact tttctagcga cattatattg aatgcgaatg gacagccgat
    10141 cccgttttcc agtcaagtat tgagttgcat catggtgatg ctttgggtat ttgttggtat
    10201 tgaaggagcc tctgtcgtag gttcacgagc aaaaaaacaa tcagatgttg ggaaagcaac
    10261 gattctaggg atattcggct tgattattat ttacgtcttg gtttccatgc ttccatatgg
    10321 gacaatgacg atgaatcagc tgcaaacgat caaggcgcag cctgctatgg gctatgtgtt
    10381 tgaaatgatg gttggtaaat ggggagcagt cgtgatcaac ggcggtttga tcatttcttt
    10441 agttggtgtt tggctctcat ggacgatctt accgattgaa acaatgggaa acatggctga
    10501 agacggactt ttgcctaaag catggggaaa actcaacaaa aatggggcac caacttatgc
    10561 tatcgtattg acgacgttat gtacgaatgt ctttttactt tcattattat tcactgacag
    10621 tgcttataac tttgcttaca cacttggtac agcagctatt ttcttcacgt ggcttttctt
    10681 aggattgtac caaatgaaac tttcttatca gcggaaagaa tgggtccaat ttttagtagg
    10741 gcttttagca agtgcctttc aagtatgggc tatgctagtc gtagccttta atgaagtaat
    10801 gcttgttctt gttcttttct tgccaggcat ttatttttac ctcaaagcac gtaaggagca
    10861 aaacgagcct gtacaacata tccacttaga taagatttta cttataggtg tgacgatggt
    10921 cggagtgatt gcaatggttt tatttgccac tgggatgctt cacgtataga agtataaaag
    10981 aagtataaag agaggttgtg agcctgtcgt ttattcaaaa acatactagg aaaggagctg
    11041 tgacacaaat gttgtcacag ctcctttttc agaataaacg gtgtttaaga gacagccaat
    11101 cggcaataag gctgaacgtc tctttcacag cctctttttt gatgagtcat catacatttt
    11161 ttcgcttaaa tacgagatat cctaatgcta agaacaaaac gatgtaagct aaattaccga
    11221 atagcatttg attagtagaa agatgagtca attgcttcat gatctcttcg tttccaattt
    11281 ggttttgcag attcaacata ctgatcggat tccatttgat ccactcccat ttttggatga
    11341 gtgcaataag gataccagat atgattgacg aagcaaaata aaagacgatt cctgctgaga
    11401 tggatgcgcc ggaactattg ataaagcaag caatcatcaa cactaaactt aaaatcagcc
    11461 ataaaccgat ataactaccc aaagtataag tgaacaagct ttggatcagt gtttgaccag
    11521 tagaaacctt ctccgtgaag gaaatcgaag ggaaaagaat cagtttcatc aacacagtga
    11581 caataatagc taaaaggtat aaataaacag aataaataac taatgtgatc catttgctta
    11641 ctaatatttc tcctcgagaa tactttcgat aaaggagatt tttcaatgta ccatattgtg
    11701 cttccatcga aatgatcgtg cttgcagctg caatcatgat aaagacaatc caagatgtgg
    11761 cactaaaaag ctgagcagtc atttcaactg gatcaatgat cgtcgtgtat tttttggcta
    11821 gaagtgctgt tcctatgagc aaaacaacta atagaacaga taaaacagca gttgactttt
    11881 tcttgatcaa tttaaataa
//
LOCUS       DAYYOA010000316         6459 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.132, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000316 DAYYOA010000000
VERSION     DAYYOA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.132"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..2598
                     /locus_tag="ACHBKA_003024"
     CDS             151..2598
                     /locus_tag="ACHBKA_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9446610.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            2828..3895
                     /gene="rlmN"
                     /locus_tag="ACHBKA_003025"
     CDS             2828..3895
                     /gene="rlmN"
                     /locus_tag="ACHBKA_003025"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9446611.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            3885..4091
                     /locus_tag="ACHBKA_003026"
     CDS             3885..4091
                     /locus_tag="ACHBKA_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446612.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(4088..5191)
                     /locus_tag="ACHBKA_003027"
     CDS             complement(4088..5191)
                     /locus_tag="ACHBKA_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9446613.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5744..6292
                     /gene="aac(6')"
                     /locus_tag="ACHBKA_003028"
     CDS             5744..6292
                     /gene="aac(6')"
                     /locus_tag="ACHBKA_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9446614.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2157 a         1107 c         1383 g         1812 t
ORIGIN      
        1 ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa
       61 aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat
      121 aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa
      181 aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt
      241 ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta
      301 ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt
      361 cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt
      421 aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat
      481 gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat
      541 ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt
      601 ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca
      661 gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg
      721 ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc
      781 aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa
      841 gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag
      901 aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat
      961 acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct
     1021 gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt
     1081 tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag
     1141 gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat
     1201 caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa
     1261 agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc
     1321 gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc
     1381 gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg
     1441 attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa
     1501 gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga
     1561 cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt
     1621 agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc
     1681 catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc
     1741 atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa
     1801 aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat
     1861 atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca
     1921 accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc
     1981 aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat
     2041 atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat
     2101 gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat
     2161 tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag
     2221 aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt
     2281 caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa
     2341 gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca
     2401 cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt
     2461 tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca
     2521 gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg
     2581 gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa
     2641 agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt
     2701 atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag
     2761 tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga
     2821 aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt
     2881 tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa
     2941 acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc
     3001 ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac
     3061 agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca
     3121 agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt
     3181 ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca
     3241 gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt
     3301 cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca
     3361 tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag
     3421 tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat
     3481 ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt
     3541 cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat
     3601 cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt
     3661 agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc
     3721 agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga
     3781 tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga
     3841 tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta
     3901 tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca
     3961 acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa
     4021 ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag
     4081 caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt
     4141 ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa
     4201 tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat
     4261 tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa
     4321 acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc
     4381 cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca
     4441 tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat
     4501 ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt
     4561 gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat
     4621 ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag
     4681 catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa
     4741 tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt
     4801 tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga
     4861 agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac
     4921 cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat
     4981 ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca
     5041 aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta
     5101 cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg
     5161 tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg
     5221 ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc
     5281 taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc
     5341 ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta
     5401 gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat
     5461 tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga
     5521 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
     5581 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
     5641 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
     5701 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
     5761 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
     5821 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
     5881 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
     5941 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
     6001 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
     6061 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
     6121 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
     6181 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
     6241 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
     6301 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt
     6361 gaatttttgt gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca
     6421 gaggttgtga aaaagtcgct tagtttcgag tagtaagaa
//
LOCUS       DAYYOA010000317         5613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.149, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000317 DAYYOA010000000
VERSION     DAYYOA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.149"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1026)
                     /locus_tag="ACHBKA_003029"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="ACHBKA_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACHBKA_003030"
     CDS             1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACHBKA_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM9446615.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="ACHBKA_003031"
     CDS             1851..3005
                     /locus_tag="ACHBKA_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM9446616.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="ACHBKA_003032"
     CDS             3220..4188
                     /locus_tag="ACHBKA_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM9446617.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="ACHBKA_003033"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="ACHBKA_003033"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM9446618.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="ACHBKA_003034"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="ACHBKA_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DAYYOA010000318         4272 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.172, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000318 DAYYOA010000000
VERSION     DAYYOA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.172"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..1297
                     /locus_tag="ACHBKA_003035"
                     /pseudo
     CDS             170..1297
                     /locus_tag="ACHBKA_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            1395..1583
                     /locus_tag="ACHBKA_003036"
     CDS             1395..1583
                     /locus_tag="ACHBKA_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM9446619.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1641..1994)
                     /locus_tag="ACHBKA_003037"
     CDS             complement(1641..1994)
                     /locus_tag="ACHBKA_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9446620.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2525..2947
                     /locus_tag="ACHBKA_003038"
     CDS             2525..2947
                     /locus_tag="ACHBKA_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9446621.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2944..3174
                     /locus_tag="ACHBKA_003039"
     CDS             2944..3174
                     /locus_tag="ACHBKA_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9446622.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3672..3872
                     /locus_tag="ACHBKA_003040"
     CDS             3672..3872
                     /locus_tag="ACHBKA_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM9446623.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3899..>4272
                     /locus_tag="ACHBKA_003041"
     CDS             3899..>4272
                     /locus_tag="ACHBKA_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM9446624.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKK"
BASE COUNT         1456 a          680 c          782 g         1354 t
ORIGIN      
        1 aagttatatt atcataatac cacaaaatca caaattttca ttcaattatc attttcaatt
       61 gcattttata aatcattttg tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc
      121 cattttgaag tacattttgt cattttgtac tccaaaactg aactacttac ttgcatatat
      181 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
      241 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc
      301 ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg
      361 agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac
      421 aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat
      481 ctcagatagt gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact
      541 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
      601 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
      661 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
      721 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
      781 cttaaatcaa tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg
      841 attatatggt tggaatgtga cggattgtaa aatctgtttt aagtacggtt tatactatag
      901 ccctgttagt actccagcag attttcggat gcttactcct attgtactgg agcaagcctt
      961 tagaaaagct ggaacagaat tgttagagcc atatcttagt tttaaagttt atgcaccaca
     1021 ggaatatctt tcacgggcat ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac
     1081 tcaactgaaa aataatgagg tcattattat tggagaaatt cctgctcgat gtattcaaga
     1141 ttatcgcaat gatttaactt tttttacaaa tgggcttagt gtttgtttag cagagctaaa
     1201 aggatatcag gttaccactg gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat
     1261 agataaagta agatatatgt tcaataaaat aacttagtgc gttttatgtt gttatataaa
     1321 tatggtttct tattaaataa gatgaaatat tctttaatat agatttgaat taaagtggaa
     1381 aggaggagat tgttattata aactacaagt ggatattgtg tcctatttgt ggaaataaaa
     1441 caagactacg aatacgagtg gatactatac ttaaaaattt ccctttatac tgccccaaat
     1501 gtaagaacga aactttaatt aatgttcaaa aaatgaatat aataacaatc aaagagccag
     1561 acgccaagac gcagagccga taatttgaga aatgaaactc tcatcttatc ggctcttttt
     1621 gtttatctga attttactga ctagccttca atatttcttg cttcattgat accttttgct
     1681 agtgcttcca ttaaggatag ttctttgtct gtaaagctat ccatgtattt ttctacctgt
     1741 aaccttcgag tgcttttaat caaattatct gtaggtaaga aaaattcatc aatagataca
     1801 tggagtaatg atacaagatc atacagcact tgtgtacttg ggtgttgtcc tttgttttct
     1861 atatttgtta aataacgtgg gtcaatttca atcattgttc ccacttgttc acgagtgaga
     1921 ccccgtttca ttcgagcctc tttaatcgct aaaccaacgg ctctaaaatc gtatttatct
     1981 tcttttttac gcatactaaa tcacctctat acattttact gttcctatat aaccataaac
     2041 aggtatagaa aaacatgtta tatagtttat gagttcctat agaacactgt ggaatttatt
     2101 aatgcgatga atccaatttc aaatagaaag gtcgctgatt ttatttacct atacaacttt
     2161 ataaaattta gtcatactcc tagaaagtag ataaaattgg cgacttaatg gagggaaaag
     2221 tcgccatgag aaagataatc tgtcatctag ttctcttaat taagttctta tcaaaaaaac
     2281 ttatccacct gtactggata gatatgacca tcaatgtggt tttgcagatt acatagatag
     2341 aaaaatcaaa gcatatgaac agagaaaaca atctgtttat gtgctttttt ggtcacttaa
     2401 taaatatctt ttaggatttc tcttcattta tgcaacaaat cccccgttcg tgtcggggtt
     2461 tagtatgaag ggagataaag aagcacatgc cactcccttt cacgacagaa agggggtgaa
     2521 aagcatgaaa ccatcttctt ttcaagaaac catagaaaat cagtttgact atatctgcaa
     2581 acaagttatt gaagatgaac gaaaagatta cttcaaacat ctagacagac tatcagcaaa
     2641 agaagtctcg ttttctgata tagataacta ccttgtcaac tctttttcta ctgttgatac
     2701 gtacgtcact gatttccaac tatttaaatt atttggatta agaatcaatg ttgaaagtga
     2761 tttattaagt gaagccctgc gtcatctttc agagaaaaaa cggaacatta ttttacttca
     2821 ttactttatg gatatgagtg atgtggagat tgcggagtta ctaaatttga atcgttcgac
     2881 tgtttatcgt catcgaataa gtggactagc catgattaaa gaattcatga aggagtgtaa
     2941 agaatgaaaa cagcttatcc aagggttccc ttccccctca ttgtaaaggc gacagatggc
     3001 gacgtggaag caattaatca gattgttaaa cattatcgag ggtatacctc aaaacgttca
     3061 ttacgtcgca tgacagacga atatggaaat tctcatatgg ttattgatga aaccctacgt
     3121 ggcagaatgg aaacgaaact catctctaag attttatcgt tcgaaattaa atagtcccat
     3181 tttttcgtgg aagcgtgtcg aatcgtatcc acgcttcccg atttgggagg tttgttattc
     3241 catcaaagca tattgtgtgt tcagtaggtt ttgataggct aacaagccaa acgttctttg
     3301 acaaccaaat aaaggcaatc gaatacgttt cggtaagaaa agagctgatg aactaacacg
     3361 ccatgaccta caaaattagt gagcgattca tgttagtaat tcgaaagtaa tctttagcag
     3421 gattgctgcc atgactttct tatcgtgata atgatactcc cgtatagcca tagtccgagc
     3481 gttagaagcg tcacaggcaa tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc
     3541 tgtgagcttt gctagagctg ttcgattgct aagatattct tctcttgtca gtgtattatt
     3601 ataaaactat ttgatataat tataggtgaa tattggagtt ttcctagcct ataattggga
     3661 aaggagtaat aatgataacg gtcacaaaaa aggatttagt tgagctagga tatggaactt
     3721 ctttttcagc agacatcata aaaaaagcaa aagaattgat gattacaaaa gggcatatct
     3781 actatcaatc tcgaaagcta gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa
     3841 ttcggttttc aaatgaaaac aggtctgagt agtttttgca cagtaaggag taaaaactat
     3901 ggttaaagac ccaataaaaa aagcaaagaa tggaacctat tattttcgtg caaatctagg
     3961 ttatgattct aaagggaaaa aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga
     4021 agcaagagaa gaatattcga aactgctatt gatgaaacca gaagaattaa gtgagaataa
     4081 ggataaaatg acgtttaaac attatatctt tgaaattttc cttccatggt ataagacaca
     4141 ggtaaagctc agaacatatg aaaatcgctt accaactatt aagaagcatt tctcttattt
     4201 tgataaaatg gctgtttccg atattgagcc tattgatgtg caaaattggc aactaaaatt
     4261 atcaaagaag tg
//
LOCUS       DAYYOA010000319         4134 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.174, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000319 DAYYOA010000000
VERSION     DAYYOA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.174"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1677
                     /locus_tag="ACHBKA_003042"
     CDS             <1..1677
                     /locus_tag="ACHBKA_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM9446625.1"
                     /translation="LKYFYFYHLNNGIWNAKTVWIRKGTVINKMLDFLDLKYPSITSI
                     TEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSYYVTNLKQFMEFYEN
                     FYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNM
                     DSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGR
                     ISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPE
                     YIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVAL
                     LIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHA
                     HAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTN
                     NGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCT
                     SKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            1684..2061
                     /locus_tag="ACHBKA_003043"
     CDS             1684..2061
                     /locus_tag="ACHBKA_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM9446626.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            2215..2997
                     /locus_tag="ACHBKA_003044"
     CDS             2215..2997
                     /locus_tag="ACHBKA_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM9446627.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(3123..3854)
                     /gene="erm(A)"
                     /locus_tag="ACHBKA_003045"
     CDS             complement(3123..3854)
                     /gene="erm(A)"
                     /locus_tag="ACHBKA_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFM9446628.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(3912..3971)
                     /locus_tag="ACHBKA_003046"
     CDS             complement(3912..3971)
                     /locus_tag="ACHBKA_003046"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFM9446629.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
BASE COUNT         1473 a          600 c          762 g         1299 t
ORIGIN      
        1 ttgaaatatt tttattttta ccatttaaac aatggaatat ggaatgcaaa aactgtctgg
       61 attagaaaag gaacagtaat taataaaatg ttagatttct tagatttaaa gtatcctagc
      121 attacttcaa ttactgaagt tcctattgaa aaagcaatga cggagtatag aacttatttg
      181 acaaaacggg gtgttagaat taccactact aattataaga ttactgctaa tcaagaaaaa
      241 acacctgtaa aagctaattc ctactatgtt actaatctaa aacaatttat ggagttttat
      301 gagaactttt attttgatgg agaggagtgg gataaagacg tttgggatag acgtaactta
      361 cctttgccag atgataaggt taacccaaca caatatgaat atacaattaa ctttaaaggg
      421 tttcggaata catattttaa acaacttgta aaaagatatt gtaagttgag attgaacatg
      481 gatagctttt cctatgtaag tgatattgcc caaagactta aagagttctt taattttctg
      541 gacatgaaat ttaaacaagt tcagagagta caccaattaa cgagagtgga aattgaagca
      601 tatttaagtg aactaaacat gatgggaata aaacctagta caataactgg gaggatctct
      661 atattggaag gactatttag tacccttctt aggctagaat gggatgatgt tccttccaaa
      721 atattaattt attctgagga ctatccgaaa ataccaagag caaaaccacg ctttatagat
      781 gaattcgtcc tagagcaatt gaacagtcat cttgataaat tacccgaata tatagctacg
      841 atgactatga ttgttcaaga atgtggaatg aggataagtg aattgtgcac cttgaaaaaa
      901 ggctgtctat tagaggacaa agatggagat ttctttttaa agtattatca atggaaaatg
      961 aaaaaggagc atatagttcc aatatctaaa gaggtagctt tacttattaa agttcgggaa
     1021 gataaagttt cagaggaatt tccagatagt gaatacctct ttccaagaaa agatggatcg
     1081 ccattaaaac aagaaacatt tagaggtgag ttaaataaat tagcttatga gcaaaatata
     1141 gtggataaat caggtgagat ttatagattc catgcccatg cctttcgcca tacagtagga
     1201 acaagaatga ttaacaacgg gatgccccag catattgtgc agaaattttt ggggcatgaa
     1261 agcccagaaa tgacaagcag atacgctcat atctttgatg aaactctaaa aaatgaattt
     1321 actaaatttc aggaaaaact ggttaccaat aatggagatg tgcttgatct agatgaagat
     1381 aatgaagtcg atgatgtaga gcttcaatgg ttcaagaaaa atataaatgc acaagtgctt
     1441 ccaaatggtt attgtagatt gccagtagta gcaggtggtt gtccacatgc gaatgcatgc
     1501 ttagattgca ctcacttctg taccagtaag caattcttac cacagcacga agaacagtta
     1561 gagcgtacag aagagttatt agccatagct aaggataaac aatggcaaag acaagtagag
     1621 actaatagcc gtgttaaaga gcgtttagaa caaatcattg gaagtttgac ggggtaatta
     1681 tcaatggata aacaagttag aaatacaaca gaaattgtac gtttggcgaa gcagaaatca
     1741 aaaaagacaa gggaaaaagt agacaaagcg atttctaaat tttcgattga aggtaaagtt
     1801 attaatttta attcaatagc aaaggaagct aatgtttcta aatcatggct ttataaggaa
     1861 cacgatatta ggcaaagaat cgaatccctt cgtgagcgtc aaataacagc aaatgtagtc
     1921 tcaaaaccca agaaaagttc tcgttcggag gaaatcctta ttaaaacctt aaaaagaaga
     1981 gtaatggaat tagaaaaaga aaataaaaaa ttacagaacc aaattcaaaa attatatgga
     2041 gatctgtata ataaagaata attattaatc tgtagacaaa ttgtgaaagg atgtacttaa
     2101 acgctaacgg tcagctttat tgaacagtca tttaggtata tgtccaatct agggtaagta
     2161 aattgagtat caatataaac tttatatgaa cataatcagt tacgaggtga aatcatgagc
     2221 aatttgatta acggaaaaat accaaatcaa gcgattcaaa cattaaaaat cgtaaaagat
     2281 ttatttggaa gttcaatagt tggagtatat ctatttggtt cagcagtaaa tggtggttta
     2341 cgcattaaca gcgatgtaga tgttctagtc gtcgtgaatc atagtttacc tcaattaact
     2401 cgaaaaaaac taacagaaag actaatgact atatcaggaa agattggaaa tacggattct
     2461 gttagaccac ttgaagttac ggttataaat aggagtgaag ttgtcccttg gcaatatcct
     2521 ccaaaaagag aatttatata cggtgagtgg ctcaggggtg aatttgagaa tggacaaatt
     2581 caggaaccaa gctatgatcc tgatttggct attgttttag cacaagcaag aaagaatagt
     2641 atttctctat ttggtcctga ttcttcaagt atacttgtct ccgtaccttt gacagatatt
     2701 cgaagagcaa ttaaggattc tttgccagaa ctaattgagg ggataaaagg tgatgagcgt
     2761 aatgtaattt taaccctagc tcgaatgtgg caaacagtga ctactggtga aattacctcg
     2821 aaagatgtcg ctgcagaatg ggctatacct cttttaccta aagagcatgt aactttactg
     2881 gatatagcta gaaaaggcta tcggggagag tgtgatgata agtgggaagg actatattca
     2941 aaggtgaaag cactcgttaa gtatatgaaa aattctatag aaacttctct caattaggct
     3001 aattttattg caataacagg tgcttacttt taaaactact gatttattga taaatattga
     3061 acaatttttg ggaagaataa agcgtcctct tgtgaaatta gagaacgctt tattacttta
     3121 atttagtgaa acaatttgta actattgaaa atagaaagaa attgttcctt cgatagttta
     3181 ttaatattag tgacatttgc atgcttcaaa gcctgtcgga attggttttt agtgaaaaga
     3241 acacgatatt cacggtttac ccacttataa acaaaagatc gatacttttt gtagtccttc
     3301 tttgaaatca atggttgatg tcgttcaaga acaatcaata cagagtctac acttggctta
     3361 ggatgaaaat atagtggtgg tacttttttg agcattttta tatccatctc caccattaat
     3421 agtaaaccca aagctcgttg cagattttgc aatcttttcg caaatccctt ctcaacgata
     3481 agatagctat atttagcctg actttcaaag gtaattcttt tgacaatatc cgtactgatg
     3541 ttataaggaa tattaccata tatcttatag tttatatgtt ttgggaagga aaattttaga
     3601 atatccgttt gaatcacttt tatattctca gaggggttta ccgcttcttt agtcacttga
     3661 cataagcctc catcaatttc tatagcagta actgatcgac tcattttgac tagctctttg
     3721 gtaaaatgtc cttttcctga tccgatttct attacgttgt cttgtttact gatattcgtg
     3781 tgattcaata tttcttttac atgcttttta gaagtaataa aattttgcgt gtctttaggg
     3841 tttttctggt tcattataac cttctcctta ctggttataa tgaactgaca ttaagcgttc
     3901 attataacca attaattttg atttggttga taacgaactt tattaataac aaatatagaa
     3961 aaagtaccca tacttttatc tcctttccat ttttttcatt atgaatgaga taaagtaatt
     4021 tctactactg cgatactggt gcacataatt gaaacctcct tatgtaactg aatatgattg
     4081 tatcatcttt tcattttaga ataaaccttt atatgtaatt ttgcacttga cact
//
LOCUS       DAYYOA010000320         4104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.175, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000320 DAYYOA010000000
VERSION     DAYYOA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.175"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1532
                     /gene="msr(C)"
                     /locus_tag="ACHBKA_003047"
     CDS             54..1532
                     /gene="msr(C)"
                     /locus_tag="ACHBKA_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9446630.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1579..2025
                     /locus_tag="ACHBKA_003048"
     CDS             1579..2025
                     /locus_tag="ACHBKA_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9446631.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2224..2739)
                     /locus_tag="ACHBKA_003049"
     CDS             complement(2224..2739)
                     /locus_tag="ACHBKA_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9446632.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2856..4058
                     /locus_tag="ACHBKA_003050"
     CDS             2856..4058
                     /locus_tag="ACHBKA_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9446633.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1222 a          699 c          892 g         1291 t
ORIGIN      
        1 tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa catatggaaa
       61 atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta gaactatcta
      121 ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa aatggtgtcg
      181 gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat gggaaaatcc
      241 aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa gcaaaggacc
      301 taaatttgga attagccagt catttccagt taagactgga agaaacttca gagcggaaat
      361 ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct tatgaacaag
      421 ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat cgactgattg
      481 aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc tattttctag
      541 atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag ttttcgggga
      601 attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta cgagaagcag
      661 agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag aaaaaacaag
      721 cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga ctggatcggt
      781 tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat gcgaaaacat
      841 tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg aaacaaatcc
      901 gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg ggacaaaatg
      961 tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag ttttctttag
     1021 gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta ttagaacata
     1081 tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa gtatatcagc
     1141 aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg agacaaacag
     1201 agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct caggaaactt
     1261 tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg attgctttgc
     1321 tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt attgatatgg
     1381 caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt ttgtttactt
     1441 ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata aaagggcaga
     1501 aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt cgtgacaaat
     1561 ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt tacaacaatg
     1621 atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca tcaagatctt
     1681 catttatcaa gtttgttgat tggtctagtg cctggattcg tggtggcttt cattttggat
     1741 gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta aactaccagt aaatccagaa
     1801 agcaaggtcc aaatgatctt aactatttca tgcttgatgg ttttaggaat ggtgacatgt
     1861 atgtctttat tcggtgtgat catggaacaa ggaatacctg atcaattatt ctctgcttat
     1921 atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat catcgttggc
     1981 ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa cactgttatt
     2041 tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg aatgcagttc tagaaaaata
     2101 gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc tttttctttt ccaactgtat
     2161 tgctctttta ttgaagataa tttgctcttc aatagatatt tctagaaagg ttctgtttat
     2221 cccttactca aaaaactgct gcatttgttc tcttacttgc tgacaccatt ggagttcctg
     2281 ctgacaaagc ataattctgc gctggatcac aacataacgt ccaaattctt gtttcttgtc
     2341 tagcgagagg tcattcaatt ttttttgatt tttttgcatg attttgttca gctgttgttc
     2401 tctagtgaat agcagtccag tagctgttgt tttatcgagc gtcgaaaaag catagatttt
     2461 tgctagaaat tcatcttttt gcgtaggagc gggagtttcc tctttgatcc attcctctac
     2521 aagagcacgt ccttcatcag tcaagtggta gatctttttt tgagagtgct cgtctttgtc
     2581 caaaacggtc acataaccct gctggtctaa cttggcaagc gttgtataga tctgactgtg
     2641 atgggcttcc caaaaaattg tgatcagctg cttcaattca tacccagaac gtggtttgga
     2701 taaaagcacg cataataata tataggctaa agtattcata aaaatcctac ctctcgtctt
     2761 attttcttta ttatatcaca gtaaagtttc cacgttttac atgtgcatat tgacatgtaa
     2821 aataaatcat gtatgatgct aatagagaga aagggatgga aatattggag aatcaaatag
     2881 atatacaaga acaaacaaat gaagaaacaa aggggaaaag ttcagcgcta gttgtagcag
     2941 ctacctgtat gatcgctttt atgggagtgg gtctagtaga cccaatctta aaaacgattg
     3001 ctgcacagtt aaacgctacg ccagctgaaa cgaccctgtt atttactagt tacatgttag
     3061 tcactggtgt ggtgatgcta tttacaggtt tcttatctag tcggatcggt ttgaaaaaaa
     3121 cattgatgac aggattgttt atcatcgttg tattcgctgg tcttggcgga ttatctggga
     3181 atatcggaat gctgattggg ttacgtgccg gctggggtgt cgggaatgct ttatttgtct
     3241 ctactgcttt agcaacgatc gtcagtattt tagttggtaa cacagaaaaa gcaatcatga
     3301 tgtatgaagc agcacttggc tgcggaatgg ctgttggccc tctagtcggt ggggtcttgg
     3361 gcagtggttc atggcgttat ccattttttg gggtagcatt tttgatgttt tgcggtttta
     3421 tagccctagc ggtactttta cctgaaactg agaaaccaaa gagaaaagta ggcctttggg
     3481 aaggtgtcaa agcgttaagc aatcataaac ttcgttcagt tggactaatc gcattgttat
     3541 ataatttcgg attctttact ttacttgcgt atgcgccatt tttattgatt ggctattctg
     3601 aattagaagt cggttttgta ttttttggct ggggtgtgct tctggctatt gcttcgattt
     3661 tcgttgcacc ggctcttgag cggaagttta cgacttatcg gacaatttta gcagcatttg
     3721 tcttattcat tatttgtttg ctgttattag gagctggaac ttctatgcca gtccttgtac
     3781 cgatcagcat tgtgttagca ggatttttcc aaggaattat caatacactg ttgacgacga
     3841 ttgctatgga aataccaggt ctgcaacgaa acgttgcttc gtccagctat agttttgtcc
     3901 gctttttcgg tggagcttta gcaccattta ttgcaggtgt gattggtgaa catattggtg
     3961 aaagctatgc gttttatttt gcaggtgcca ttgttcttat cagtgtatgt tgtatcattt
     4021 atcaccgtaa ttattttgct acaaatgaag aagaataaga aatcagaggt tgtgaaaaag
     4081 ctgtcctgca tcaagtcata agaa
//
LOCUS       DAYYOA010000321         2601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.199, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000321 DAYYOA010000000
VERSION     DAYYOA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.199"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..376)
                     /locus_tag="ACHBKA_003051"
     CDS             complement(<1..376)
                     /locus_tag="ACHBKA_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM9446634.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVM"
     gene            469..818
                     /locus_tag="ACHBKA_003052"
                     /pseudo
     CDS             469..818
                     /locus_tag="ACHBKA_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1090..1380)
                     /locus_tag="ACHBKA_003053"
     CDS             complement(1090..1380)
                     /locus_tag="ACHBKA_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9446635.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1588..2280)
                     /locus_tag="ACHBKA_003054"
     CDS             complement(1588..2280)
                     /locus_tag="ACHBKA_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9446636.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          776 a          477 c          363 g          985 t
ORIGIN      
        1 ccattactgt caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat
       61 ttattttctc aaaaacatac ttgcaaagta atactacaat tcctaacata atcgtagaat
      121 ttaataattt ccatagcata aagtgtttag aaaagaacat taagaatagt tctataataa
      181 ttcttgaact ccatgtctta taatgttcta tcgaagcacc caaatagcca agttcatcta
      241 atctacttgc ataaactacg tcatccgctc tcatagtcgg catatacata ttaaagagta
      301 aaaagatacc ccaaatccct attagtaaca agatcagttc tttgttattc gttatttttc
      361 tattatgttt gttcattttt atactccttc ttatatttcc taatagagta taacatagct
      421 ttaagcatta cttaaagata acttaaaagt tatcttttaa atacttaatt gttaaatacc
      481 ttatttgatg caaaagaaat ctacgatagt caacaaaaaa attttcatta tatccagaaa
      541 aatttttctt agttaaggac ctatggattg cagtaatgca gaattcgtcg aataaactgc
      601 caaagacata caatcatatt gcttttaaaa ttgacgaaca agatatagat tcgtttgtct
      661 caaaaatcca aatgttaggt ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg
      721 cttcttcaat ttacttttac gactatgata atcacctctt cgagttacat accggtactc
      781 tccaggaacg tttaaaatcc tacaattcca ctacctgaga ctagtaatga ctagtctctt
      841 tttatggcct ctgatccccc cattgagcga atactaatag ggcttcgaat tgatctgttc
      901 aatctttaag caatatccct caaatgagtt tggaaagttt agtgattcat cacaaatact
      961 taaatacatg tagttatata tagattgagg ttcttgtgag cgtatgctcg tgtgggttca
     1021 aatcccatcg tctgcatcca tctttccaaa acaagcggct aggacatttt tgtctcagcc
     1081 gcttgttttc tagtttattc tttcatcttg tcggaccttt tttcttgggt atagactttt
     1141 ctcatattct accacaattt tgggtttccc taacacatga taggatctca caacaaattg
     1201 ctttctatta ttataataac ctgtaacgac taacttcatc ccttcgtcta cttcagctag
     1261 gaagttcaaa ctatgggaag caatcaaaca attgacccca tttaaactga aacggatcaa
     1321 cggtctggca ctaacttgca aagtacggat cttatctact gttccatatt ttttctccat
     1381 aaccttcacc tcaataccat tatacgaaca tacattccct ttgtaaagaa gaaactctgt
     1441 tttatctttt tgactttttg tctattacct ttcttaaaat catattggca cttcctgcga
     1501 agatgaagga catgtactaa aaaaaagagg cagcagtata gattactgtt gcttctttac
     1561 taatttaggt ttacttgtta agaaagatta tttacgcaaa tgttcttcaa caatttgctg
     1621 agatttatca gctgtatctt ccacttcatc ttttgctttt tctaactttt gaatatattg
     1681 tttcaagctt gagttttcag cagttgcttc gtctaatttc ttttgtgtat ctgttaactg
     1741 agtttctaca tttgtttttt cttgtgtcag tgttgcgatt tgttgattca aaccatttac
     1801 agtattttgt aattctgaaa gttttgcttg tgcttcttgt agtcttgctg tccaatctgc
     1861 attcagatga ttgatttcat catttttttg gttgatctca tctagtttga cattgatttc
     1921 attttgtttg ctttgtagtt gtgcatcctt gctgactaat tgactttcta agttttgttt
     1981 ttctgctgcc gattgatttt tttggttttc taattcattt acttttgttt ggtattcttg
     2041 gacatctgcc tttgaatctt gcaatgtctg ttctttttgt gctagtcgag cggtaagatc
     2101 tgtaagcgct tgctctttat ctgtcaatgt atgatcaagc tgtgtcagat tctcattgat
     2161 tgtatcaata tctgtctctc caccccacaa aataatttca tttgcgtacc gcgtgcctgt
     2221 gtagccaatt agacatagaa atgcaacgac aatcccacca gtaataaatt tctttttcat
     2281 ttgcatagct ccttaatcca atacttttac atgctaaaag taagtgatct caaacacaat
     2341 gtcaagcgtt tttaaattac atgtgtaatt ttaatataaa tactatcaaa tactcattta
     2401 atcctccttt tttatctatt taaaaaatct tttttataga gaaaaatgca ggttaatact
     2461 ttcttaaata aatatttatg gtgaacaaaa acaaaaaagc aagttttatt aaacgtaacc
     2521 cttttcattt catagattac tgtatttatt acaaaaaacg cggatttgtc ataaattgtc
     2581 atgcttcttt tacctatgaa a
//
LOCUS       DAYYOA010000322         1584 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.235, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000322 DAYYOA010000000
VERSION     DAYYOA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.235"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..623
                     /gene="dfrG"
                     /locus_tag="ACHBKA_003055"
     CDS             126..623
                     /gene="dfrG"
                     /locus_tag="ACHBKA_003055"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9446637.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(695..>1584)
                     /locus_tag="ACHBKA_003056"
     CDS             complement(695..>1584)
                     /locus_tag="ACHBKA_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9446638.1"
                     /translation="NDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRFVDGLNVD
                     APYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHFICQVNKT
                     KSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEEAISKDGE
                     RYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQIR
                     RYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTPA
                     QRLGITDKQFDWKDIIYFK"
BASE COUNT          521 a          234 c          296 g          533 t
ORIGIN      
        1 gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg
       61 agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt
      121 tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga
      181 atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac
      241 atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa
      301 gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt
      361 caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag
      421 aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc
      481 atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat
      541 ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg
      601 aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag
      661 cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt atatctttcc
      721 aatcaaattg tttatctgta atccctaatc tttgagcagg tgtcaaaacc tttttatccg
      781 ctgatttaaa agggcgacaa aagttgtagt aagttcttag aattgtaatc gcaaattgag
      841 catacttcgg attgaagtta gcgtaaatat agctcttctt atcaccacga gctgttgtta
      901 atggtctttc aagagatgat atataacgtc ttatctgttg aataaagcta ttggtcgaat
      961 ggtcattaac atttaatagc atctttgcga tttcgtttgg ttccagagca gataaatccg
     1021 ttctacaatc aactgaataa aatcctttat ccttagtggc taatggatgt ttgatagggt
     1081 tatcggcata tttgcgatat ctttctccat ctttactaat agcctcttcg tggaattgat
     1141 gacttgttag taattcagtt agataatgag atgccaaagt ccgtagaaac tttgttttga
     1201 atcctctgat atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat tcttttaagc
     1261 attgttttct gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca tcgctcaacc
     1321 ttaattcttt cgtgaatact ctataaaagg cgtttctaat agagaagtca tcatcactaa
     1381 tcattcgcca ttcgtcggca ttaaccattt gtttaattaa ccaatagtga gcagttgtag
     1441 tataaggagc atcaacattt aaaccatcga caaatctact cctattagta attttcccta
     1501 attcgtgaag ataagcattc ctattttcgc tatcgttagc ggatggttct tgtggataat
     1561 aagaccaatc taacctatca tttt
//
LOCUS       DAYYOA010000323         1504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.240, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000323 DAYYOA010000000
VERSION     DAYYOA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.240"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACHBKA_003057"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACHBKA_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACHBKA_003058"
     CDS             394..477
                     /locus_tag="ACHBKA_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM9446639.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHBKA_003059"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHBKA_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9446640.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAYYOA010000324         1421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.246, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000324 DAYYOA010000000
VERSION     DAYYOA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.246"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1350
                     /locus_tag="ACHBKA_003060"
     CDS             <1..1350
                     /locus_tag="ACHBKA_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFM9446641.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          114 c          239 g          471 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
     1381 gacggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
LOCUS       DAYYOA010000325         1350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5997
            SAMEA115708092-rid22598903.guided.251, whole genome shotgun
            sequence.
ACCESSION   DAYYOA010000325 DAYYOA010000000
VERSION     DAYYOA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708092
            Sequence Read Archive: ERR13197817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290209.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290209.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:48:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708092-rid22598903.guided.251"
                     /isolate="VRE5997"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACHBKA_003061"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACHBKA_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACHBKA_003062"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACHBKA_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM9446642.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
